NanoNano--BioBio--TechnologyTechnology

Speaker : Speaker : ShenShen HsingHsing HsuHsuDate : 7/13 2004Date : 7/13 2004

ATP as the ATP as the bioenergybioenergy moleculemolecule

The History of ATPThe History of ATP

• In 1929, Dr. K. Lohmann found the ATP molecule.

• In 1939-1941, Dr. Fritz Lipmann provie that ATP is the Bioenergy molecule.

• In 1948, Dr. Alexander Todd synthesis ATP in chemistry.

• The formation and consumption of ATP are proceed by the enzyme ATP synthetase and ATPase.

1997 Nobel Prize in Chemistry1997 Nobel Prize in Chemistry

http://vm.nthu.edu.tw/science/shows/1997nobelchem/index.html

Dr. Jens C Dr. Jens C SkonSkon work for Nawork for Na++/K/K++

ATPaseATPase

http://vm.nthu.edu.tw/science/shows/1997nobelchem/index.html

Dr. Paul D. Dr. Paul D. PoyerPoyer work for the work for the mechnismmechnism of of ATPaseATPase

Binding Change Mechanism

http://vm.nthu.edu.tw/science/shows/1997nobelchem/index.html

John E Walker work for XJohn E Walker work for X--Ray crystal Ray crystal

http://vm.nthu.edu.tw/science/shows/1997nobelchem/index.html

Classify the Classify the ATPaseATPaseV-type ATPase vs F-type ATPase

Nature Review VOLUME 3 | FEBRUARY 2002 94-103

Subunits composition of the VSubunits composition of the V--ATPaseATPaseand Fand F--ATPaseATPase

S.Kawasaki-Nis hi et al./FEBS Letters 545 (2003) 76-85

ATPaseATPase as a biomotor(Fas a biomotor(F11--ATPase)ATPase)

BiomotorBiomotor (F(F00FF11--ATPase)ATPase)

SCIENCE VOL 286 26 NOVEMBER 1999

SCIENCE VOL 290 24 NOVEMBER 2000

Direct evidences for rotational ATPase

The rotation revolution in different The rotation revolution in different conditionsconditions

VV--ATPaseATPase is also a is also a biomotorbiomotor

PANS, March 4, 2003 100 2312–2315

The rotation of VThe rotation of V--ATPaseATPase

The Rotation of The Rotation of ATPaseATPase

Rotational modelRotational model

ATPaseATPase Interaction and AssembleInteraction and Assemble

• How are V0 V1 (F0 F1) assembling?

• How are subunits of V0interaction?

• The EM of V0 and V0V1section.

• The X-ray crystal structure.

Membrane TopologyMembrane Topology

JBC Vol. 274, No. 45, Issue of November 5, pp. 31804–31810, 1999

ApplicationsApplications

•Biophysical evaluation nano-propeller.

•Biochemical switch control motor function.

•Fueling system light driven ATP production.

•Nano-Bio-generator Change the chemical potential to electric power.

PP--ATPaseATPase

The Journal of Experimental Biology 198, 1–17 (1995)

AFMAFM--Atomic force microscopyAtomic force microscopy

AFM-contact mode AFM-tapping mode

AFM used in membrane proteinAFM used in membrane protein

• 2-D crystal• TEM confirmed• AFM scanning

2D Crystal2D Crystal

Protein Protein extrationextration

SCIENCE VOL 288 7 APRIL 2000

Calculate Calculate TransmembraneTransmembrane DomainDomain

SCIENCE VOL 288 7 APRIL 2000

Conformational change in membrane Conformational change in membrane channelchannel

Nature structural biology • volume 7 number 9 • september 2000

AFM in Ku protein and DNAAFM in Ku protein and DNA--PKPK

PNAS. Vol. 94, pp. 4267–4272, April 1997

RNA polymerase at work

Nature structural biology • volume 7 number 9 • september 2000

ConclusionConclusion• Analysis of protein structure is a nano-

biotechnology.

• Like ATPase, could V-PPase work as a biomotor?

• AFM is a powerful technology for membrane protein.

• 2D crystal is a central technology for membrane protein in AFM analysis.