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CellProfiler is an open-source image processing package,
designed for image analysis. With this free software you can
analyze a series of images and get quantitative results.
For more information, please contact:Kairos Instruments, LLC.520
William Pitt WayPittsburgh, PA 15238
Cell Counting – ( Automated cell counting in CellProfiler )
Telephone: 412-519-9613Website:
http://www.kairosinstruments.comEmail:
[email protected]
You need a single image or a series of images for analysis.
CellProfiler provides you with a number of sample images and
tutorials to get started.
Select path to the images
Select path to the output
Select + to add image processing procedures to your
‘Pipeline’
This area of the start up screen is where you build a
Pipeline,
a procedure the program follows to process a series of
images.
You can also save and reload existing Pipelines
(Startup Screen)
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For more information, please contact:Kairos Instruments, LLC.520
William Pitt WayPittsburgh, PA 15238
Telephone: 412-519-9613Website:
http://www.kairosinstruments.comEmail:
[email protected]
To view any image in your selected series, just double click on
the image name.
Pipeline
Each step in a Pipeline will need some settings. In this case,
LoadImages is the first ‘Module’ in the Pipeline. This Module will
load the image/s for analysis.
Loading image 100
Each Module has a Help file, to explain each setting in the
module.
Set to 10.jpg, the module will load every 10th image for
analysis
Press Analyze Imagesto run the Pipeline
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For more information, please contact:Kairos Instruments, LLC.520
William Pitt WayPittsburgh, PA 15238
Telephone: 412-519-9613Website:
http://www.kairosinstruments.comEmail:
[email protected]
Output:
Each Pipeline will process from one image to a series of images
and produce visual and analytical results
In this result, the Pipeline produced images showing the
selected cells and a Cell Count, 16 cells.
In this series of images, every 10th fluorescent image was
counted, then sent to Excel for further analysis.
The Pipeline processed 42 images in 237 seconds, (4
minutes).
/ColorImageDict > /JPEG2000ColorACSImageDict >
/JPEG2000ColorImageDict > /AntiAliasGrayImages false
/CropGrayImages true /GrayImageMinResolution 300
/GrayImageMinResolutionPolicy /OK /DownsampleGrayImages true
/GrayImageDownsampleType /Bicubic /GrayImageResolution 300
/GrayImageDepth -1 /GrayImageMinDownsampleDepth 2
/GrayImageDownsampleThreshold 1.50000 /EncodeGrayImages true
/GrayImageFilter /DCTEncode /AutoFilterGrayImages true
/GrayImageAutoFilterStrategy /JPEG /GrayACSImageDict >
/GrayImageDict > /JPEG2000GrayACSImageDict >
/JPEG2000GrayImageDict > /AntiAliasMonoImages false
/CropMonoImages true /MonoImageMinResolution 1200
/MonoImageMinResolutionPolicy /OK /DownsampleMonoImages true
/MonoImageDownsampleType /Bicubic /MonoImageResolution 1200
/MonoImageDepth -1 /MonoImageDownsampleThreshold 1.50000
/EncodeMonoImages true /MonoImageFilter /CCITTFaxEncode
/MonoImageDict > /AllowPSXObjects false /CheckCompliance [ /None
] /PDFX1aCheck false /PDFX3Check false /PDFXCompliantPDFOnly false
/PDFXNoTrimBoxError true /PDFXTrimBoxToMediaBoxOffset [ 0.00000
0.00000 0.00000 0.00000 ] /PDFXSetBleedBoxToMediaBox true
/PDFXBleedBoxToTrimBoxOffset [ 0.00000 0.00000 0.00000 0.00000 ]
/PDFXOutputIntentProfile () /PDFXOutputConditionIdentifier ()
/PDFXOutputCondition () /PDFXRegistryName () /PDFXTrapped
/False
/Description > /Namespace [ (Adobe) (Common) (1.0) ]
/OtherNamespaces [ > /FormElements false /GenerateStructure true
/IncludeBookmarks false /IncludeHyperlinks false
/IncludeInteractive false /IncludeLayers false /IncludeProfiles
true /MultimediaHandling /UseObjectSettings /Namespace [ (Adobe)
(CreativeSuite) (2.0) ] /PDFXOutputIntentProfileSelector /NA
/PreserveEditing true /UntaggedCMYKHandling /LeaveUntagged
/UntaggedRGBHandling /LeaveUntagged /UseDocumentBleed false
>> ]>> setdistillerparams> setpagedevice
