New Products 2013 Version 4

8/13/2019 New Products 2013 Version 4

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EMD Millipore—with the expertise of Calbiochem ®, Chemicon®, and Upstate®

EMD Millipore is a division of Merck KGaA, Darmstadt, Germany

Epigenetic Regulators Panel - AcetylationCat. No. 324893

Histone acetylation/deacetylation has been implicated in

many biological processes, such as cell differentiation and

survival, double-strand DNA repair, cell cycle progression,

malignant transformation, tumor cell proliferation, cardiac

function and remodeling, and plant acclimation to cold

stress. EMD Millipore’s new Epigenetic Regulators Panel

contains 18 potent and selective small molecules and

enables a coordinated study of the interplay between

acetylation and deacetylation processes.

Epigenetic Regulators Panel - MethylationCat. No. 324894

DNA methylation is an important epigenetic marker of gene

repression. It plays important roles in heterochromatin

formation, long-term silencing of repetitive elements,

X-chromosome inactivation, differentiation of embryonic

stem cells and the establishment and maintenance of

imprinted genes. EMD Millipore’s new Epigenetic Regulators

Panel contains 12 potent and selective molecules and is

an ideal starting point for probing DNA methylation using

chemical genetics.

Small but powerful: regulating gene expressionwith small molecule epigenetic modulatorsGiven the emerging importance of epigenetic regulation and

dysregulation in disease states, it is not surprising that over

a dozen small molecule epigenetic modulators have been

reported in the literature as effective epigenetic modulators

and are either in preclinical or clinical trials1. A few are

already on the market. These small molecules span three

categories of modulators: “readers”, “writers”, and “erasers”

of epigenetic marks. The affected marks include histone

methylation, histone acetylation, and DNA methylation.

In addition to their therapeutic utility, these molecules are

also valuable for elucidating signaling networks and activity

of various enzymes involved in epigenetic regulation. Unlike

genetic perturbations, these small molecules enable dynamic

assays and precise dosing. EMD Millipore is offering these

valuable reagents, with full characterization of purity and

potency, to advance studies of gene regulation.

NeuroscienceCancerSignaling Cell Structure Stem Cell

ANTIBODIES, ASSAYS, SMALL MOLECULES, INHIBITORS, AND PROTEINSNew VOLUME 4 I 2013

products

Epigenetics &

Gene Regulation

1. Lu Q, Quinn AM, Patel MP, Semus SF, Graves AP, Bandyopadhyay D, Pope AJ, Thrall

SH. Perspectives on the discovery of small-molecule modulators for epigenetic

processes. J Biomol Screen. 2012 Jun;17(5):555-71.

HAT

Silenced Genes Gene Expression

HDAC

Hyperacetylation

Apoptosis

Inhibitors

http://www.millipore.com/?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309



EMD Millipore is a division of Merck KGaA, Darmstadt, Germany

We’re validated.

We’re guaranteed.We’re published.We create the antibodiesmost cited by the research community.

Reput(Ab)leAntibodies

Researchers trust our antibodies because we are a thoughtful

antibody producer, not a reseller. We’re selective about offering

the best antibodies based on the expertise of Chemicon® andUpstate®, internal R&D teams and collaborations with leading

institutions. We guarantee our antibodies because of a stringent

validation process that produces the highest quality antibodies on

the market today.

We provide the most reliable, defensible, and publishable antibody

performance, because, ultimately, it’s not about our reputation.

It’s about yours.

Put the most reputable antibodies to work for you.

www.emdmillipore.com/Ab

http://www.millipore.com/antibodies/flx4/antibodieshome?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309





VOLUME 4 I 2013

Description Host Species Reactivity Key Applications Cat. No.

Antibodies

Anti-BANF1, clone EPR7669 Rabbit Hu WB, IHC MABE537

Anti-Bmi-1, clone EPR3745(2) Rabbit Hu WB, IHC MABE480

Anti-Bonzo Rabbit Hu WB, IHC ABE604

Anti-Centrin-2 Rabbit Hu, Ms, Rt WB, IC, IHC, IP ABE480

Anti-Centrin-3 Rabbit Hu, Ms, Rt WB, IP, IC, IHC ABE481Anti-CHD-4, clone 3F2/4 Mouse Hu, Ms WB, IHC, ChIP, IF MABE455

Anti-dimethyl G9a (Lys185) Rabbit Hu WB, PIA, IP ABE489

Anti-dimethyl Histone H3 (Arg17),

symmetric

Mouse Hu WB, DB, IC MABE246

Anti-dimethyl Histone H3 (Lys36) Rabbit Hu WB, DB, ChIP 07-369-I

Anti-FOXO4, clone EPR5442 Rabbit Hu WB, FC MABE539

Anti-H2A.Z.2.2, clone 1H11-11 Rat Hu WB MABE347

Anti-HIRA, clone EPR7416 Rabbit Hu WB, IF MABE565

Anti-Histone Deacetylase 6,

clone EPR1698(2)

Rabbit Hu WB, IHC MABE546

Anti-Histone H1°, clone 34 Mouse Hu, Ms, Rt, Bov, Xenopus WB, IC, IHC, FC MABE446

Anti-Histone H3.3, K27M mutant Rabbit Ms, Hu WB, ChIP ABE419

Anti-hMCM2, clone 2-40 Mouse Hu, Ms, Rt WB MABE203

Anti-IDH Rabbit Hu WB, IHC(P) ABE454

Anti-JMJD2D Rabbit Hu, Chimp WB, IHC, IP ABE499

Anti-KDM1B Rabbit Hu, Ms WB ABE495

Anti-LSD1, clone EPR6825 Rabbit Hu, Ms, Rt WB, IHC MABE545

Anti-Mad1, clone BB3-8 Mouse Hu WB, IF MABE867

Anti-Mad2, clone AS55-A12 Mouse Hu WB, IF MABE866

Anti-monoubiquitin H2A (Lys119) Rabbit Hu, Ms WB, ChIP, IC ABE569

Anti-Myb Binding Protein 1A Rabbit Hu WB, IC, ChIP ABE577

Anti-PERP Rabbit Hu WB, IC ABE606

Anti-PHF19 Rabbit Hu WB, IP, ChIP ABE992

Anti-phospho-FOXO4 (Ser262),

clone EPR2315(2)

Rabbit Hu WB MABE555

Anti-PTBP2 Rabbit Hu, Ms, Rt WB, IP ABE431

Anti-RAG-1 Rabbit Hu WB, IC, IP ABE440

Anti-Sirt1(Sir2), clone 3-10 Rabbit Ms WB, IHC MABE426

Anti-Ski, clone G8 Mouse Hu WB, IHC MABE442

Anti-SPT5 Rabbit Hu WB, IP ABE443

Anti-SUV39H1, clone EPR4960(2) Rabbit Hu WB MABE552

Anti-TCF-4, clone 5F9.1 Mouse Hu WB, IHC MABE361

Anti-TET2 Rabbit Hu WB ABE595

Anti-TET2, clone hT2H 21F11 Mouse Hu, Ms WB, IP MABE462

Anti-THAP11 Rabbit Hu, Ms, Rt WB, IP, ChIP, IHC ABE567

Anti-THRAP3 Rabbit Hu IP, IC, WB ABE487

Anti-TIF1-α Rabbit Hu, Ms WB, IC ABE434

Anti-TIP60, clone 3F9 Mouse Hu WB, IHC MABE430

Anti-UBE2I, clone 1B10 Mouse Hu, Mky WB, IF, IHC, FC MABE429

Anti-USP9X, clone 3H11.1 Mouse Hu, Ms, Rt WB, IHC MABE352

Anti-UTX Rabbit Hu, Ms WB, IC ABE409

Anti-WBP-7 Rabbit Hu, Ms, Rt WB ABE500

Anti-WDR5, clone 7B11 Mouse Hu WB MABE428

Anti-ZEB1 Rabbit Hu WB, IC, IF ABE596

ChIPAb+™ Acetyl Histone H3 (Lys56) Rabbit Hu WB, DB, ChIP 17-10259

Milli-Mark® Anti-acetyl-Histone H4-PE Rabbit Hu FC FCABS326PE

Epigenetics & Nuclear Function

LEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine,Mky=Monkey, Can=Canine, Prim=Primate, Por= Porcine,Chimp=Chimpanzee, Rhe Mac=Rhesus Macaque,Ham=Hamster, Chk=ChickenApplications: FC=Flow Cytometry, IC=Immunocytochemistry,IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry(Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation,

WB=Western Blotting, ChIP=Chromatin IP,ELISA=Enzyme Immunoassay, DB=Dot Blot, PIA=PeptideInhibition Assay, CS=Cell Sorting, MeDIP=Methylated DNA IP,CC=Cell Culture, BM=Bisulfite Modification

For published studies using this new product,

visit: www.emdmillipore.com

For publications on using these small molecules,

visit: www.emd4biosciences.com

Anti-Histone H3.3,K27M mutant(Cat. No. ABE419)

In a recent Science paper titled:Inhibition of PRC2 activity by a gain-of-

function H3 mutation found in pediatric

glioblastoma* the Allis laboratory and

colleagues (representing Rockefeller,

Princeton, Duke and the University of

Pennsylvania) used EMD Millipore’s newly

released anti-Histone H3.3 K27 mutant,

rabbit polyclonal antibody (Cat. No.

ABE419) to explore epigenetic control in

pediatric glioma and other pathologies.

The authors discovered that certain

human gliomas containing the missense

mutation Lys27Met (K27M) in histone

H3.3 displayed lower overall amounts of

H3K27me3 both in vivo and in vitro . The

Histone H3K27 mutant-specific antibody

from EMD Millipore was used in Western

blotting, immunohistochemistry and ChIP

analysis to reveal the expression of the

gain-of-function mutation and correlate it

to the disease state. The researchers propose

that K-to-M substitutions may represent a

mechanism to alter epigenetic states in a

range of pathologies.

NOTE: To help you extend and complement

these studies we have also just released arecombinant H3K27M recombinant protein.

See the the Proteins, Enzymes and Reagents

Section on the next page.

*Lewis PW et al. Science. 2013 May 17;340(6134):857-61.

PUBLICATION HIGHLIGHT

http://www.millipore.com/catalogue/item/MABE537?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309


http://www.millipore.com/catalogue/item/ABE604?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309






http://www.millipore.com/catalogue/item/07-369-I?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309






































http://www.millipore.com/catalogue/item/17-10259?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309

http://www.millipore.com/catalogue/item/FCABS326PE?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309


http://www.emdmillipore.com/life-science-research/?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309
























http://www.millipore.com/catalogue/item/FCABS326PE?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309






























VOLUME 4 I 2013





LEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim=Primate, Por= Porcine,Chimp=Chimpanzee, Rhe Mac=Rhesus Macaque, Ham=Hamster, Chk=ChickenApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry(Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB=Western Blotting, ChIP=Chromatin IP,

ELISA=Enzyme Immunoassay, DB=Dot Blot, PIA=Peptide Inhibition Assay, CS=Cell Sorting, MeDIP=Methylated DNA IP,CC=Cell Culture, BM=Bisulfite Modification


Antibodies - Trial Size

Anti-acetyl-Histone H3 (Lys14),

clone EP964Y, Trial Size

Rabbit Hu, Rt WB, IHC(P), IC,

IP, DB

04-1044-S

Anti-acetyl-Histone H3 (Lys56), Trial Size Rabbit Hu WB, DB, ChIP 07-677-IS

Anti-Histone H3, (Unmodified Lys4),

clone CMA301, Trial Size

Mouse Hu, VertebRtes WB, IC, ELISA, IP,

FC, DB

05-1341-S

Anti-monomethyl-Histone H3 (Lys9),

Trial Size

Rabbit Hu, Ms, Chk DB, IC, PIA, WB 07-450-S

Anti-monomethyl-Histone H3 (Lys9),

Trial Size

Rabbit Hu WB, IC, PIA, DB ABE101-S

Anti-trimethyl Histone H3 (Lys4),

clone CMA304, Trial Size

Mouse Hu, VertebRtes ELISA, IC, IP, WB,

Multiplexing, DB

05-1339-S

Anti-trimethyl-Histone H4 (Lys20),

Trial Size

Rabbit Hu, Ms, Rt IP, WB, ChIP, DB 07-463-S

Kits & Assays

CpGenome™ Direct Prep Bisulfite

Modification Kit (200 Reactions)

BM 17-10452

CpGenome™ Direct Prep Bisulfite

Modification Kit (50 Reactions)

BM 17-10451

Proteins, Enzymes, & ReagentsHuman Recombinant Histone H3.3 E. Coli ChIP, Activity

Assay

14-1063

Human Recombinant Histone H3.3 K27M E. Coli ChIP, Activity

Assay

14-1064

Epigenetics & Nuclear Function (continued)

Description Details Cat. No.

Small Molecules & Inhibitors

5-Azacytidine A cell-permeable ribonucleoside analog of cytidine that is reported to readily

incorporate into RNA and DNA, and inhibit protein synthesis. Causes DNA

methyltransferases depletion, DNA hypomethylation and DNA damage.

5.04317.0001

CRM1 Inhibitor IV,

KPT-251

A cell-permeable, irreversible, triazole-containing heterotricyclic CRM1 inhibitor

that effectively blocks CRM1-mediated nuclear export (4 h 1µM in U2OS cells)

via covalent interaction with CRM1 NES- (nuclear export signal) binding groove

cysteine (Cys528 in human).

5.00505.0001

Ezh2 Inhibitor II, EI1 A cell-permeable indolocarboxamide compound that acts as a potent and selective

inhibitor against Ezh2/PRC2 (Polycomb repressive complex 2; Ezh2/SUZ12/EED/

AEBP2/RbAP48) HMTase activity (IC50

/substrate = 15 nM/H3K27me021-44 and

13 nM/H3K27me221-44, respectively, against human wt or Y641F Ezh2-containing

PRC2; [SAM] = 1 µM) in a cofactor SAM-competitive manner.

5.00561.0001

Histone Lysine

Demethylase

Inhibitor VII, GSK-J1

A pyridinyl-pyrimidinylaminopropanoic acid that effectively inhibits KDM6 family

H3K27m3 demethylases JMJD3 and UTX (IC50

= 18 and 56 µM, respectively, by

MALDI Mass detection) in an α-ketoglutarate-competitive, peptide

substrate-non-competitive manner. Recommended for cell-free assays only.

420204-10MG

JQ1 Enantiomers Set A cell-permeable I-BET (Cat. No. 401010) class of thienodiazepine derivative whose

(S)-(+), but not (R)-(-), enantiomer is shown to target both bromodomains

(BD1 & BD2) of BET family members BRD2, BRD3, BRD4, and BRD6/BRDT in a

Kac- (ε-N-acetylated lysine) competitive manner.

500586-1SET

L3MBTL3 MBT

Domain Blocker,UNC1215

A cell-permeable pyrrolidinyl-piperidinyl-benzamide that effectively competes

against H4K20Me2 peptide for L3MBTL3 binding by targeting the first two L3MBTL3MBT domains (K

d = 120 nM in binding studies using recombinant 3MBT fragment).

5.00557.0001

Ezh2 Inhibitor II, EI1(Cat. No. 5.00561.0001)

EMD Millipore has recently introduced

a new inhibitor of Ezh2/PRC2 (Enhancer

of zeste homolog 2/Polycomb repressivecomplex 2) methyltransferase activity.

In a 2012 PNAS paper titled:

Selective inhibition of Ezh2 by a small

molecule inhibitor blocks tumor cell

proliferation*

Qi and colleagues showed that this inhibitor

exhibited about 142-fold greater selectivity

over Ezh1 and >10,000-fold selectivity over

several other histone methyltransferases

(G9a, Suv39H2, Set7/9, CARM1, SmyD2,

SETD8, NSD3, SETD2, MLL, and Dot1L).

They also report that this Ezh2 inhibitor

blocked the growth of diffuse large B cell

lymphoma cells carrying Ezh2 mutations

and diminished H3K27me3 and H3K27me2

levels without affecting H3K27me1.

* Qi, W. et al. 2012. Proc. Natl. Acad. Sci, USA 109,

21360-21365.

Perform intricate in vitro manipulations of

histones, nucleosomes, and chromatin with

our portfolio of purified recombinant histones,

native nucleosomes, highly active histone-

modifying enzymes, and broad range of

modified peptide substrates. To see more, visit:www.emdmillipore.com/epiproteins

PUBLICATION HIGHLIGHTON SMALL MOLECULES:



http://www.millipore.com/catalogue/item/04-1044-S?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309

http://www.millipore.com/catalogue/item/07-677-IS?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309



http://www.millipore.com/catalogue/item/ABE101-S?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309







http://www.millipore.com/catalogue/item/5.04317.0001?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309



http://www.millipore.com/catalogue/item/420204-10MG?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309

http://www.millipore.com/catalogue/item/500586-1SET?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309



http://www.millipore.com/catalogue/module/c130633?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309


http://www.millipore.com/catalogue/item/500586-1SET?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309












http://www.millipore.com/catalogue/item/ABE101-S?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309



http://www.millipore.com/catalogue/item/07-677-IS?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309


http://www.millipore.com/catalogue/module/c130633?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309






VOLUME 4 I 2013





Cancer

LEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim=Primate, Por= Porcine,Chimp=Chimpanzee, Rhe Mac=Rhesus Macaque, Ham=Hamster, Chk=ChickenApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry(Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB=Western Blotting, ChIP=Chromatin IP,

ELISA=Enzyme Immunoassay, DB=Dot Blot, PIA=Peptide Inhibition Assay, CS=Cell Sorting, MeDIP=Methylated DNA IP,CC=Cell Culture, BM=Bisulfite Modification


Antibodies

Anti-ACVR1 Rabbit Hu IHC ABC330

Anti-Ambra1 Rabbit Hu, Ms WB ABC131

Anti-Atg13 Rabbit Hu WB ABC130

Anti-CLEC2A Rabbit Hu WB ABC328

Anti-CRK Mouse Hu, Ms, Rt WB MABC172Anti-CRKL Rabbit Hu, Ms, Rt WB, IP ABC242

Anti-CRTC1, clone 1B5 Mouse Hu WB, IC, FC MABC691

Anti-DHODH Rabbit Hu WB, IHC(P) ABC138

Anti-EIF4B, clone 1F5 Mouse Hu, Ms WB, IC, FC, IHC MABC666

Anti-ENC1, clone EPR5406(B) Rabbit Hu WB, IHC MABC685

Anti-EPB41L3 (DAL1), clone 12D12.1 Mouse Hu WB, IHC MABC156

Anti-EPHB4 Rabbit Hu WB ABC257

Anti-ETV4, clone 1A2G3 Mouse Hu WB MABC681

Anti-FLI1 Rabbit Hu, Ms WB ABC219

Anti-GLI1 Rabbit Hu, Rt WB, IHC(P) ABC217

Anti-Gli-1, clone EPR4523 Rabbit Hu WB, IHC MABC678

Anti-GPC3, clone 9C2 Mouse Hu, Ms WB, IF, IHC, FC MABC667

Anti-IRF-5, clone EPR6094 Rabbit Hu WB, FC MABC686

Anti-KLF4, clone 1E6 Mouse Hu IHC, WB MABC631

Anti-KPNA2 Rabbit Hu, Rt WB, IHC ABC342

Anti-LPL, clone 2C5 Mouse Hu WB MABC674

Anti-MATK, clone 9D7 Mouse Hu WB, FC MABC682

Anti-MUM1, clone 4G10 Mouse Hu WB, IHC MABC640

Anti-Napsin A, clone EPR6252 Rabbit Hu WB, IHC MABC643

Anti-NLRP10, clone 8H2 Rat Hu WB, FC, IC MABC293

Anti-NPC1 Rabbit Rt, Ms WB ABC133

Anti-nSMase1, clone EPR6718 Rabbit Hu WB, IF MABC687

Anti-Nucleostemin, clone 5C3.1 Mouse Hu WB, IC MABD97

Anti-NUP96, clone EPR6678 Rabbit Hu WB, IHC MABC689

Anti-OTUD7B, clone 90-46-126 Mouse Hu WB MABC281

Anti-PALB2 Rabbit Hu WB, IHC ABC331

Anti-PDCD1LG2 Rabbit Hu WB, FC ABC221

Anti-phospho AMBRA1 (Ser52) Rabbit Hu WB, PIA ABC80

Anti-phospho MLKL (Thr357) Rabbit Hu WB, PIA ABC234

Anti-phospho ULK1 (Thr574) Rabbit Hu WB, PIA ABC212

Anti-PIDD, clone 1511.1 Mouse Hu, Ms, Rt WB, IC MABC189

Anti-PLAP, clone EPR6141 Rabbit Hu WB, IHC MABC644

Anti-PLK-4, clone 6H5 Mouse Hu WB, IC, IP MABC544

Anti-PSP94, clone EPR7345 Rabbit Hu WB, IHC MABC645

Anti-PTGFR, clone EPR5844 Rabbit Hu WB MABC642

Anti-RIP, clone EPR4689 Rabbit Hu WB MABC688

Anti-RRM2, clone 4D1.2 Mouse Hu WB, IC MABC154Anti-RUNX1, clone 2B5 Mouse Hu WB MABC679

Anti-SCARB1 Rabbit Hu WB, IHC, IF ABC326

Anti-SCCA, clone EPR5157 Rabbit Hu WB, IHC MABC646

Anti-SDF-1 Rabbit Hu, Ms, Rt, Mky WB ABC96

Anti-SFRP1, clone EPR7003 Rabbit Hu WB, IHC MABC647

Anti-SFTPC Rabbit Hu, Rt, Ms WB, IHC(P) ABC99

Anti-phospho AMBRA1(Ser52)(Cat. No. ABC80)

EMD Millipore’s newly released anti-

phospho AMBRA1 (Ser52) rabbit polyclonal

antibody (Cat. No. ABC80) was recently

published in an important study identifying

a new target for mTOR and a key new

positive regulation loop in autophagy in a

Nature Cell Biology paper titled:

mTOR inhibits autophagy by controlling

ULK1 ubiquitylation, self-association and

function through AMBRA1 and TRAF6*

The authors demonstrated that mTORinhibits AMBRA1 by phosphorylation

under non-autophagic conditions. Upon

induction of autophagy, AMBRA1 enhances

ULK1 kinase activity and stability through

its Lys-63-linked ubiquitination and

interaction with TRAF6, thus detaching

from the anchoring dynein complex. The

authors used the anti-phospho AMBRA1

(Ser52) antibody in Western blotting

and immunocytochemistry to identify

AMBRA1 phosphorylation in the complex

during autophagic induction. Elucidating

the interactions in this autophagosome-

related protein complex may reveal apositive feedback mechanism for sustaining

autophagy in a continuous loop and may

have significant therapeutic implications.

*Nazio F et al. Nat Cell Biol. 2013 Apr;15(4):406-16.

PUBLICATION HIGHLIGHT



http://www.millipore.com/catalogue/item/ABC330?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309




http://www.millipore.com/catalogue/item/MABC172?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309























http://www.millipore.com/catalogue/item/MABD97?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309














































































Akt Inhibitor XVI,

AT7867

A cell-permeable pyrazolo compound that acts as a potent inhibitor against Akt

(IC50

= 32, 17, and 47 nM against Akt1, Akt2, and Akt3, respectively), PKA (IC50

= 20

nM), and p70S6K (IC50

= 85 nM) in an ATP-competitive manner (Ki = 18 nM using

AKT2), with much reduced potency toward RSK1 (IC50

>100 nM) and a panel of

13 other cellular kinases (IC50 >1 µM).

124033-10MG

Bcr-abl Inhibitor IV,

Imatinib

A cell-permeable, orally bioavailable 2-phenylaminopyrimidine derived compound

that competitively binds to ATP-binding site of Abl and stabilizes the inactive

conformation, and acts as a highly potent and reversible inhibitor of Abl (IC50

= 38,

25 and 25 nM for v-Abl, Bcr-Abl, and c-Abl, respectively). Also shown to block the

activity of PDGFR and c-kit (IC50

= 50 and 100 nM).

5.04595.0001

CXCR4 Antagonist

IV, TF14016

A 14-aa internally disulfide-bonded peptide that potently competes against SDF-

1α/CXCL12 for CXCR4 binding (IC50

= 0.91 nM; [SDF-1] = 100 nM) and protects

MT-4 cells against X4-HIV strain HIV-1IIIB infection (EC50

= 4 nM in 5 d;

MOI = 0.01) with no significant cytotoxicity (CC50

= 56 µM; 5 d).

5.00507.0001

ERK Inhibitor VIII A cell-permeable pyrimidylpyrrole compound that acts as an active site-targeting,

highly potent and selective ERK1/2 inhibitor (KI <2 nM against Erk2; [ATP] = 65 µM).

Orally available in both mice and rats in vivo .

328011-10MG

Gemcitabine, HCl A bioavailable, moderately toxic, deoxyctytidine analog with anti-metabolite and

anti-tumor properties. Shown to be effective against a variety of tumors and blocks

the growth of cancer cells in culture (IC 50 = 8.7, 57, 3.7, and 36 nM for L1210,

CEM, Caco2, and HeLa cells, respectively).

5.04594.0001

HIF Inhibitor V A cell-permeable phenoxyacrylamido compound that inhibits hypoxia-induced

cellular HIF-1α accumulation in a manner similar to LW6 (Cat. No. 400083) by

upregulating Hippel-Lindau tumor suppressor gene product pVHL, but with much

higher potency (IC50

= 120 nM vs 2.44 µM, respectively, against hypoxia-induced

HRE reporter activity in HCT116 cells

5.00498.0001

IDH1 Inhibitor, R132

Somatic Mutant-

Specific

A cell-permeable phenyl-glycine analog that acts as an IDH1 (Isocitrate

Dehydrogenase 1) R132 somatic mutant-specific inhibitor (IC50

= 0.07, 0.16, and

>100 µM, respectively, against R132H, R132C, and wt IDH1), potently inhibiting

oncometabolite 2-HG (D-2-hydroxyglutarate) production.

410972-25MG

Mcl-1 Inhibitor,

MIM1

A cell-permeable thiazolyliminopyrogallol that selectively neutralizes Mcl-1, but

not Bcl-XL, antiapoptotic activity, by directly targeting Mcl-1 BH3-binding site,

effectively competing against Bid BH3 sequence interation with Mcl-1

(IC50

= 4.8 µM) without affecting Bcl-XL interaction with Bad or Bid BH3

sequence (IC50

>50 µM).

444130-25MG

mTOR Inhibitor XI,

Torin1

A cell-permeable pyridinonequinoline derivative that acts as a highly potent,

ATP-competitive inhibibitor against mTOR and DNA-PK (IC50

= 4.32 and 6.34 nM,

respectively), while inhibiting PI 3-K only at much higher concentrations

(IC50

=171 to 533 nM).

475991-10MG

mTOR Inhibitor XII,

Torin2

A Torin1 (Cat. No. 475991) structural analog that acts as a cell-permeable, potent,

ATP-competitive inhibitor against DNA-PK and mTOR (IC50

= 0.5 and 2.81 nM,

respectively).

475992-10MG





LEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim=Primate, Por= Porcine,Chimp=Chimpanzee, Rhe Mac=Rhesus Macaque, Ham=Hamster, Chk=ChickenApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry(Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB=Western Blotting, ChIP=Chromatin IP,ELISA=Enzyme Immunoassay, DB=Dot Blot, PIA=Peptide Inhibition Assay, CS=Cell Sorting, MeDIP=Methylated DNA IP,

CC=Cell Culture, BM=Bisulfite Modification

VOLUME 4 I 2013 Cancer (continued)


Antibodies (continued)

Anti-SOD1, clone 6F5 Mouse Hu, Ms WB, IC, FC MABC684

Anti-SOX9, clone 1B11 Mouse Hu WB, IF MABC672

Anti-STIP1, clone EPR6606 Rabbit Hu WB, IHC MABC648

Anti-Syntaxin 8 Rabbit Hu WB ABC144

Anti-TSG101, clone EPR7130(B) Rabbit Hu WB, FC MABC649Anti-TSPO Rabbit Hu WB, IHC ABC139

Anti-TXNDC5, clone 2E7/7 Mouse Hu WB, IC MABC546

Anti-VKORC1 Rabbit Hu WB, IHC ABC325

Anti-ZBED1 Rabbit Hu WB, IHC(P) ABC258



















































TLR1/TLR2

Antagonist, CU-

CPT22

A cell-permeable benzotropolone compound that competes against Pam3CSK4-

(Cat. No. 506350) for TLR1/TLR2 heterodimer binding

(Ki = 410 nM) and selectively antagonizes against Pam3CSK4-induced RAW264.7

cellular NO production (IC50

= 580 nM) without apparent cytotoxicity (up to 100

µM and 24 h).

614305-10MG



p21-Activated

Kinase Inhibitor II,

PF-3758309

A cell-permeable pyrrolopyrazole that acts as a potent inhibitor against

p21-activated kinases (Ki in nM/[ATP] in µM = 13.7/200, 18.7/40, 18.1/80. 17.1/72,

respectively, in PAK1, PAK4, PAK5, PAK6 kinase assays; IC50

= 190 and 99 nM against

PAK1 and PAK3, respectively) by directly targeting the kinase ATP-binding site in a

reversible manner (Kd = 4.5 nM using rhPAK4300-591 kinase domain).

5.00613.0001

Protein DisulfideIsomerase Inhibitor

III, PACMA 31

A cell-permeable propynoic acid carbamoyl methyl amide (PACMA) that is morepotent than PAO (Cat. No. 521000) in inhibiting PDI (protein disulfide isomerase)

thioloxidoreductase activity (IC50

= 10 and 85 µM, respectively; 1h preincubation)

by altering PDI secondary conformation via covalent modification of active site

C(397)GHC(400) motif cysteines.

539225-25MG

Rad6 Inhibitor A cell-permeable triazine compound that directly targets human E2 Ubiquitin-

conjugating enzyme Rad6B/HHR6B catalytic site via noncovalent interactions

and effectively inhibits Rad6B-Ub thioester bond formation as well as subsequent

substrate ubiquitination without affecting UbcH5B/UBE2D2-catalyzed BCA2

ubiquitination.

5.00655.0001

RubR1 Activator,

GF-15

A cell-permeable 2'-benzyloxy griseofulvin derivative that displays enhanced

mammalian cancer-selective toxicity (IC50

≤2, ≤4, ≤12, and ≥30 µM against panels

of leukemia, myeloma, solid tumors, and non-malignant cell lines, respectively),

but little antifungal activity, by activating spindle-assembly-checkpoint without

affecting microtubule kinetochore attachment.

5.00495.0001

SKP2 E3 Ligase

Inhibitor I, C1

A cell-permeable rhodanine compound that upregulates cellular p21Cip1/Waf1 and

p27Kip1 protein levels (10 µM for 16 h in 501 Mel cultures) by preventing Skp2-

Cks1-mediated substrate recruitment without affecting the F-box E3 ubiquitin

ligase Skp2 interaction with Cks1 or the SCF (Skp1-cullin1-F-box) complex.

5.00519.0001

Survivin Inhibitor

III, LLP-3

A cell-permeable, dihydropyridinone compound that selectively disrupts survivin-

Ran interaction in cell-free binding assays (IC50

<0.3 µM) and in U87 glioma cells

(by 55% in 24 h; 20 µM) via direct binding at survivin protein-protein interaction

interface.

5.00614.0001

Temozolomide A cell-permeable, orally bioavailable, blood-brain barrier-permeant MTIC

[5-(3-methyltriazen-1-yl-)-imidazole-4-carboxamide] prodrug whose in vivo DNA

alkylating activity is employed in anticancer therapy.

5.00609.0001

Cancer (continued)





LEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine,Mky=Monkey, Can=Canine, Prim=Primate, Por= Porcine,Chimp=Chimpanzee, Rhe Mac=Rhesus Macaque,Ham=Hamster, Chk=ChickenApplications: FC=Flow Cytometry, IC=Immunocytochemistry,IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry(Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation,WB=Western Blotting, ChIP=Chromatin IP,ELISA=Enzyme Immunoassay, DB=Dot Blot, PIA=PeptideInhibition Assay, CS=Cell Sorting, MeDIP=Methylated DNA IP,CC=Cell Culture, BM=Bisulfite Modification

VOLUME 4 I 2013

Inflammation & ImmunologyDescription Host Species Reactivity Key Applications Cat. No.

Antibodies

Anti-Bst2 Rabbit Hu WB ABF200

Anti-CD20, clone 2H7 Mouse Hu FC, IP, IHC MABF250

Anti-CD32, clone EP6243 Rabbit Hu WB, IHC MABF193

Anti-CD35, clone EPR6602 Rabbit Hu WB, IHC MABF194

Anti-CD38, clone 2H3-2 Mouse Hu WB, FC MABF238

Anti-CIITA Rabbit Ms, Hu WB, IHC ABF212

Anti-CSF1, clone 2D10 Mouse Hu WB, IHC MABF191

Anti-IL-33, clone 12B3C4 Mouse Hu WB, IHC MABF204

Anti-KARS, clone 8G12C1 Mouse Hu WB, IHC MABF187

Anti-Kininogen-1, clone EPR6097 Rabbit Hu WB, IHC MABF202

Anti-MDA-5, clone EPR6743 Rabbit Hu WB MABF198

Anti-NALP3 Rabbit Hu WB, IF ABF198

Anti-S1P1 Rabbit Ms WB, IHC ABF203

Anti-Tapasin, clone 7F6 Rat Hu, Rt WB, FC MABF249

Anti-TLR4, clone 13E1.1 Mouse Hu FC MABF85

Anti-TRIF Rabbit Hu WB, IHC, IF ABF201











http://www.millipore.com/catalogue/item/ABF200?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309

http://www.millipore.com/catalogue/item/MABF250?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309













































LEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim=Primate, Por= Porcine,Chimp=Chimpanzee, Rhe Mac=Rhesus Macaque, Ham=Hamster, Chk=ChickenApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry(Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB=Western Blotting, ChIP=Chromatin IP,ELISA=Enzyme Immunoassay, DB=Dot Blot, PIA=Peptide Inhibition Assay, CS=Cell Sorting, MeDIP=Methylated DNA IP,CC=Cell Culture, BM=Bisulfite Modification







InSolution™ Cytochalasin D,

Zygosporium mansonii

A 10 mM (1 mg/197 µL) sterile-filtered solution of Cytochalasin D,

Zygosporium mansonii (Cat. No. 250255) in DMSO. Cell-permeable fungal

toxin. About 10-fold more potent than cytochalasin B in inhibiting actin

filament function.

5.04776.0001

Rho Inhibitor II, Y16 A cell-permeable pyrazolidinedione compound that is shown to target

RhoGEF DH-PH domain junction with high affinity (Kd = 65 nM) and

effetively prevent RhoGEFs LARG, p115, and PDZ from interacting with

RhoA, while displaying little potency against DBL-RhoA, LBC-RhoA,

intersectin-Cdc42, or TrioN-Cdc42 interaction.

5.04043.0001

VOLUME 4 I 2013 Cell Structure

Metabolism


Antibodies

Anti-DSG2 Rabbit Hu WB, IP ABT181

Anti-ALPP, clone 3E5 Mouse Hu IHC, WB MABT457

Anti-CDCP1 Rabbit Hu WB, IHC(P) ABT180

Anti-DYNLT1, clone 7A9F4H10 Mouse Hu WB, IHC MAB1076-I

Anti-EPHB4 Rabbit Hu WB ABC257Anti-IL-1RAcP Rabbit Hu WB, IC ABT333

Anti-IQGAP1 Rabbit Hu WB, IHC(P) ABT186

Anti-L1CAM Rabbit Hu, Rt WB, IHC(P) ABT143

Anti-MSN, clone 2C12 Mouse Hu WB, IHC, FC MABT456

Anti-Oncostatin M, clone EPR7150 Rabbit Hu WB MABT459

Anti-PAK4 Rabbit Hu, Ms, Rt WB, IC ABT144

Anti-Protocadherin-10 Rabbit Hu WB, IHC(P) ABT176

Anti-Tks5, PX domain Rabbit Ms WB, IP ABT102

Anti-TSLP Rabbit Hu, Ms WB, IHC(P), IF ABT330

Anti-Versican, clone 8D8.1 Mouse Hu, Ms IHC, WB MABT161

Anti-ZEB2 Rabbit Hu WB, IC, IF ABT332


Antibodies

Anti-Apolipoprotein L1, clone EPR2906 Rabbit Hu WB, IHC MABS387

Anti-LCN13 Rabbit Ms WB ABS311

Anti-TRABID Rabbit Hu WB, IP ABS13





http://www.millipore.com/catalogue/item/ABT181?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309

http://www.millipore.com/catalogue/item/MABT457?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309


http://www.millipore.com/catalogue/item/MAB1076-I?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309













http://www.millipore.com/catalogue/item/MABS387?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309

http://www.millipore.com/catalogue/item/ABS311?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309





















http://www.millipore.com/catalogue/item/MAB1076-I?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309









LEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim=Primate, Por= Porcine,Chimp=Chimpanzee, Rhe Mac=Rhesus Macaque, Ham=Hamster, Chk=ChickenApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry(Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB=Western Blotting, ChIP=Chromatin IP,ELISA=Enzyme Immunoassay, DB=Dot Blot, PIA=Peptide Inhibition Assay, CS=Cell Sorting, MeDIP=Methylated DNA IP,CC=Cell Culture, BM=Bisulfite Modification





VOLUME 4 I 2013Neuroscience


Antibodies

Anti-ACBD3, clone EPR8623,

Rabbit Monoclonal

Rabbit Hu WB, FC MABN1045

Anti-α SNAP, clone EPR7372 Rabbit Hu WB, IHC MABN1023

Anti-Aminoacylase-1, clone EPR8444,

Rabbit Monoclonal


Anti-Annexin A3 Rabbit Hu WB, IHC(P) ABN294

Anti-Annexin A4, clone PA351-29.1.3 Mouse Hu WB, IHC, ELISA MABN480

Anti-APP-C99, clone mC99(1-7) Mouse Hu, Ms WB, IP, IC, IHC MABN381

Anti-APP-C99, clone mC99(70–80) Mouse Hu, Ms WB MABN380

Anti-ARG1, clone EPR6672(B) Rabbit Hu WB, IHC MABN1024

Anti-ARHGDIA, clone 2G3 Mouse Hu, Ms, Mky, Rt WB, IF, FC MABN689

Anti-ARNTL, clone 1C5 Mouse Hu WB, IHC MABN673

Anti-ASS1, clone 2C10 Mouse Hu, Rt, Ms, Mky WB, IHC MABN704

Anti-ATP2A1, clone EPR7321,

Rabbit Monoclonal

Rabbit Hu WB, IHC MABN1025

Anti-BAALC Rabbit Ms, Rt WB ABN254

Anti-CA9, clone 2D3 Mouse Hu WB, IHC, FC MABN713

Anti-CADM4, clone N244/5 Mouse Ms, Rt, Hu WB MABN509Anti-CAM, clone L45/30 Mouse Ms, Hu WB MABN500

Anti-cAMP, clone EP8471 Rabbit All ELISA MABN1026

Anti-Cav3.2 Calcium Channel Subunit,

clone N55/10

Mouse Ms WB MABN487

Anti-CCL2, clone 2D8 Mouse Hu, Ms, Rt, Mky WB, IF, IHC, FC MABN712

Anti-CCT2, clone 5B5C4 Mouse Hu, Ms, Rt, Mky WB, IF, FC MABN686

Anti-CD52, clone EPR3153(2), Rabbit

Monoclonal

Rabbit Hu WB MABN1047

Anti-CD73, clone EPR6115 Rabbit Hu WB, IHC MABN1027

Anti-CHD3, clone 2G4 Mouse Hu, Ms WB, IHC, IF, FC MABN694

Anti-Clcn3, clone N258/5 Mouse Hu, Ms IHC, WB MABN490

Anti-CNGA1/3, clone L36/12 Mouse Rt, Zebrafish, Ms, Fish WB, IHC MABN468

Anti-COASY, clone EPR8246,Rabbit Monoclonal


Anti-Complex I-75kD Rabbit Hu, Ms, Rt WB, IHC(P) ABN302

Anti-Cullin-5 (CUL5) Rabbit Hu WB, IHC ABN289

Anti-DCX, clone 2G5 Mouse Hu, Ms WB, IHC, IC, FC MABN707

Anti-DISC1 Rabbit Hu WB ABN46

Anti-DLK1, clone 3A10 Mouse Hu, Ms IHC, IF MABN671

Anti-ECH1 Rabbit Hu WB, IHC(P) ABN296

Anti-Ermin, clone 160 Mouse Ms, Rt WB, IC, IHC MABN323

Anti-ETV5, clone 3H3 Mouse Hu WB MABN683

Anti-FABP2, clone 9A9B7B3 Mouse Hu, Ms WB, IF, IHC, FC MABN698

Anti-FGF13A, clone N235/22 Mouse Hu, Ms, Rt WB, IHC MABN539

Anti-Fluorescent Gold Rabbit All IHC(P) AB153-I

Anti-Folate Receptor 1, clone EPR4708(2) Rabbit Hu WB MABN1067

Anti-G6PD, clone 2H7 Mouse Hu WB, IHC, FC MABN696

Anti-GADD45 β, clone EPR1235(2) Rabbit Hu WB MABN1030

Anti-GADD45B Rabbit Hu WB, IHC(P) ABN42

Anti-GIT2 Scaffold Protein, clone N83/48 Mouse Hu WB, IHC MABN469

Anti-GPI, clone 1B7D7 Mouse Hu, Rt WB, IHC, IF MABN691

Anti-GRPR Rabbit Hu WB, IHC(P) ABN295





http://www.millipore.com/catalogue/item/MABN1045?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309



http://www.millipore.com/catalogue/item/ABN294?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309




































http://www.millipore.com/catalogue/item/AB153-I?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309



























































0







VOLUME 4 I 2013 Neuroscience (continued)



Anti-Hemoglobinδ chain, clone

EPR8322(B), Rabbit Monoclonal


Anti-HK2, clone 3D3 Mouse Hu WB, IHC, FC MABN702

Anti-Human IgG1, clone EPR4417 Rabbit Hu WB MABN1055

Anti-Iduna, clone N201/35 Mouse Rt, Hu, Ms WB, IHC MABN647

Anti-IRE1p Rabbit Ms, Hu WB, IC, IHC ABN464

Anti-KDM4A, clone5H1 Mouse Hu WB, IHC, IF MABN685

Anti-Kirrel3, short isoform 3, clone

N320/48

Mouse Hu WB, IHC MABN650

Anti-LCN1, clone 10B10 Mouse Hu WB, IHC, IF MABN670

Anti-LRAT, clone M34-P1F10 Mouse Hu, Ms, Rt WB, IHC, IF MABN644

Anti-LRRK2, clone 8G10 Mouse Hu WB, IHC MABN636

Anti-LRRK2, clone N231B/34 Mouse Rt, Hu WB MABN541

Anti-LysRS, clone EPR7920,

Rabbit Monoclonal

Rabbit Hu WB, IHC, IF MABN1032

Anti-Metadherin, clone 2F11C3 Mouse Hu WB, IHC, FC MABN699

Anti-MGEA5, clone EPR7154(B) Rabbit Hu WB, IHC MABN1033

Anti-MTRR, clone EPR6110 Rabbit Hu WB, IHC MABN1086Anti-Nalcn, clone N185/7 Mouse Hu WB MABN653

Anti-NDUFA1 Rabbit Hu, Ms, Rt WB, IHC(P) ABN360

Anti-NEGR1, clone 5A4.1 Mouse Hu, Ms, Rt WB MABN360

Anti-Neurexin-1-β Mouse Hu, Rt IHC MABN607

Anti-Neuron Specific Enolase Rabbit Hu, Rt, Ms WB, IHC(P) AB951-I

Anti-NGL-1, clone N49A/21 Mouse Rt WB, IHC MABN655

Anti-NGL-2, clone N50/36 Mouse Rt, Ms WB, IHC MABN656

Anti-OCRL/INPP5b, clone N166A/26 Mouse Hu, Rt WB, IHC MABN609

Anti-Pan-GRK, clone N145/20 Mouse Ms WB MABN615

Anti-Paris/ZNF746, clone N196/16 Mouse Hu, Ms, Rt WB MABN476

Anti-PEX7, clone N232/9 Mouse Hu WB, IHC MABN659

Anti-PhyH / PAHX, clone N210/5 Mouse Hu, Ms, Rt WB, IHC MABN660

Anti-PITX3, clone EPR8868 Rabbit Hu WB, FC MABN1052

Anti-Potassium Channel Kv10.1, clone 56 Mouse Hu, Rt IHC, Activity

Assay, IF

MABN378

Anti-PPBP, clone EPR7766,

Rabbit Monoclonal


Anti-PPP1CA, clone 5E9 Mouse Hu WB MABN690

Anti-Prohibitin Rabbit Hu WB, IHC(P) ABN293

Anti-PSIP1, clone 6E4 Mouse Hu, Mky, Rt WB, IHC, IF MABN674

Anti-PSMA, clone EPR6253,

Rabbit Monoclonal


Anti-PTH1R, clone 4D2 Mouse Hu WB, IHC, IF MABN688

Anti-PTK7, clone 4F9 Mouse Hu WB, IHC MABN721

Anti-Rab10, clone 4E2 Mouse Hu, Ms WB, IF MABN730

Anti-Rab7A, clone EPR7588(B),

Rabbit Monoclonal

Rabbit Hu, Rt, Ms WB, IHC MABN1036

Anti-ROCK2, clone EPR7141(B),

Rabbit Monoclonal

Rabbit Hu, Rt WB, IHC MABN1037

Anti-S100a5, clone N176A/3 Mouse Hu WB, IHC MABN662

Anti-S100B Rabbit Hu WB, IHC(P) ABN59



























































































1

Neuroscience (continued)







VOLUME 4 I 2013



Anti-SBDS, clone EPR7820,

Rabbit Monoclonal


Anti-SCN4B, clone N168/6 Mouse Hu, Rt WB, IHC MABN654

Anti-SELK Rabbit Ms WB ABN480

Anti-SLC22A3, clone EPR6630 Rabbit Hu WB, IHC MABN1039

Anti-SMAD5, clone 3H9 Mouse Hu, Rt WB, IF, IHC, FC MABN718

Anti-SNAP-23, clone EPR8538,

Rabbit Monoclonal


Anti-SNRNP200, clone 3B6.1 Mouse Ms, Rt, Hu WB, IHC MABN297

Anti-Synaptotagmin-12, clone N277/7 Mouse Ms, Rt, Hu WB, IHC MABN519

Anti-Synaptotagmin-7, clone N275/14 Mouse Hu, Ms, Rt WB, IHC MABN665

Anti-SYPL1 Rabbit Hu WB, IHC ABN482

Anti-Tafazzin, clone N173B/13 Mouse Hu WB MABN518

Anti-Thioredoxin 1, clone EPR6110 Rabbit Hu WB, IHC MABN1090

Anti-Thorase/Atad1, clone N125/10 Mouse Ms, Rt, Hu WB, IHC MABN525

Anti-TRIP8b, clone N212A/34 Mouse Hu, Rt, Ms WB MABN666

Anti-TRPML3, clone N268/18 Mouse Hu, Ms, Rt WB MABN667

Anti-Tug, clone EPR8615 Rabbit Hu WB, IHC MABN1091

Anti-TXA2R, clone EPR7336,

Rabbit Monoclonal


Anti-UCHL3, clone EPR5332,

Rabbit Monoclonal


Anti-WIF1, clone 1G5 Mouse Hu, Ms WB, IHC, IF MABN722

Anti-WNK1, clone EPR2726(3),

Rabbit Monoclonal




(+)-WIN 55,212-2 mesylate A potent agonist for cannabinoid receptors with higher affinity to subtype

2 (CB2, Ki = 3.3 nM) than to subtype 1 (CB1, K

i = 62.3 nM).

5.04344.0001

(RS)-PPG A potent and subtype-selective agonist for group III metabotropic

glutamate receptors. 25-fold higher selectivity for mGlu8 (EC50

=0.21 µM

) than for other group III mGlu receptors (EC50

= 5.2, 4.7, and 185 µM for

mGlu4,6,7, respectively). Widely used as anticonvulsant, antidepressant,

anxiolytic, and neuroprotective agent in research.

5.04395.0001

(S)-MCPG A non-selective antagonist for group I/II mGlu receptors (minimal values

of IC50

reported = 40 and 15 µM for mGlu1 and mGlu2, respectively). Often

used as an agent to differentiate excitatory neurotransmissions mediated

by glutamate from other neurotransmitters (i.e. dopamine, acetylcholine,

and GABA in early brain development).

5.04637.0001

Adenosine A2A

Receptor

Antagonist II, SCH-58261

A highly potent and selective antagonist of adenosine A2A receptor

(Ki = 600 pM). Does not affect the activity of other adenosine receptors in

any significant manner (Ki = 287 nM; 5.0 µM, and > 10 µM for A1, A2B,

and A3 receptors, respectively). Displays neuroprotective effects in cerebral

ischaemia in rats, and shown to counteract Parkinsonian-like muscle

rigidity in rats.

119150-10MG

AIDA A relatively potent and selective antagonist for group I mGlu receptor

subtype 1 (pKB = 3.4 for mGlu1a). Complete absence of interacting with

on group II or III mGluRs, or ionotropic glutamate receptors. Widely used

in studying neural plasticity, learning and memory, and fear conditioning.

Centrally active following systemic administration in vivo .

5.04394.0001

























































2









Small Molecules & Inhibitors (continued)

ASIC Channel Modulator

II, GMQ

A positively charged guanidium-containing quinazoline that modulates

ASIC function by blocking acid-induced maximal peak current as well as

by altering ASICs pH dependence for activation and inactivation, enabling

a partial, but sustained ASIC3 activation around neutral pH (4.4% of pH

5-induced peak current with 1 mM GMQ at pH 7.4).

5.00523.0001

BINA A selective positive allosteric modulator for mGlu receptor subtype 2(EC

50 = 33.2 nM in CHO cells expressing human mGlu2). No effect on other

mGlu receptor subtypes. Exhibits antipsychotic and anxiolytic properties

in mice.

5.04463.0001

BX471 An orally active, selective antagonist for chemokine receptor 1 (CCR-1)

(Ki = 1-5 nM). Has been used in studies related to intracellular calcium

mobilization and migration of leukocytes mediated through CCR-1.

5.04345.0001

CB1 agonist ACEA A potent and highly selective agonist for CB1 receptor. Affinity for CB1

(Ki = 1.4 nM) is more than1400-fold higher than that for CB2 (KMGK =

3100 nM) receptors.

5.04191.0001

CB1 antagonist AM251 A potent and highly selective antagonist for cannabinoid subtype 1 (CB1)

receptor. Affinity for CB1 (IC50

= 8 nM, Ki = 7.49 nM) is more than 306-fold

higher than that for CB2 receptors. Has been used in a study to determine

its interaction with hippocampal neurons to enhance spatial memory in

mice.

5.04275.0001

CB2 agonist, JWH 015 A potent and highly selective agonist for cannabinoid subtye 2 (CB2)receptor. Affinity for CB2 (K

i = 13.8 nM) is more than 28-fold higher than

that for CB1 (Ki = 383 nM) receptors.

5.04274.0001

CB2 Inverse Agonist, SR

144528

A selective inverse agonist for peripheral cannabinoid (CB2) receptors

(Ki = 0.6 nM and 400 nM for CB2 and CB1 receptors, respectively). Has

been used in studies related to control of pain initiation, suppression of

inflammation, and immune activation.

5.04277.0001

CIQ A positive allosteric modulator selective for NR2C/D containing NMDA

receptors (EC50

= 2.7 and 2.8 µM for NR2C and NR2D, respectively).

Increases channel opening frequency of recombinant NR2C/D containing

NMDA receptors by two-fold without altering mean open time or

EC50

values for glutamate or glycine bindings.

5.04519.0001

CL316243 Hydrate A very potent agonist highly se lective for β3 adrenoceptors

(EC50

= 3 nM). Widely used to assess the functional roles of β3

adrenoceptors in glucose homeostasis, thermogenesis, obesity, cardiac

functions, retinal neurotoxicity, and tissue inflammation.

5.04761.0001

CNS-1102 A non-competitive, subunit non-selective, potent antagonist for

NMDA receptors (IC50

= 0.13, 0.068, 0.087, and 0.14 µM for NR2A - D,

respectively). Widely used as neuroprotective agent af ter acute traumatic

brain injury and stroke in animal researches.

5.04522.0001

CP-101,606 A highly select ive antagonist for NR2B subunit containing NMDA receptors

(IC50

= 0.039 µM for NR2B and >100 µM for NR2A, NR2C, and NR2D).

Widely used as neuroprotective, analgesic, antidepressant, and anti-

Parkinsonian agent in research.

5.04523.0001

CP-55940 A potent, non-selective agonist for cannabinoid (CB) receptors

(Ki = 0.6 - 5.0 and 0.7 - 2.6 nM for CB1 and CB2 respectively; EC

50 = 0.2,

0.3 and 5 nM for CB1, CB2 and GRP55 respectively). Often used in

reference or control trials in CB research and the effects are robust in both

behavioral tests and receptor binding assays.

5.04316.0001

Eliprodil A negative allosteric modulator selective for NR2B containing NMDA

receptors (IC50

= 1, >100, and >100 µM for NR2B, NR2A, and NR2C,

respectively). Also a potent ligand for α1 receptor (Ki = 0.013 µM), one ofthe two types of unique non-opioid, non-phencyclidine brain α receptors.

5.04539.0001

Fenobam A potent negative allosteric modulator highly selective for mGluR5

(IC50

= 87 nM; Kd = 54 nM and 31 nM at recombinant mGlu5 receptors

of rat and human, respectively). Biologically active admitted orally or

systematically. A leading therapeutic candidate for treatment of fragile X

syndrome.

5.04684.0001



































1







VOLUME 4 I 2013Neuroscience (continued)



Ghrelin Receptor Antagonist,

JMV 2959

A 1, 2, 4-triazole derived compound that acts as a potent ghrelin receptor

(GHS-R1a) antagonist (IC50

= 32 nM in a binding assay, dissociation

constant Kb = 19 nM). Shown to reduce hexarelin-induced food intake

[hexarelin (80 µg/kg, cumulative food intake (g of food/100 g of body

weight) at 6 h by acute administration (sc) of JMV 2959 (160 µg/kg)] in rats

without affecting the basal food intake.

345888-10MG

Idazoxan Hydrochloride A non-selective antagonist for α2-adrenoceptors (pKi = 8.01, 7.43, and 7.7

for α2A, α2B, and α2C receptors respectively) and imidazoline receptors.

(pKi = 5.90, 7.22 for I1 and I2, receptors respectively). Widely used in

studying functions of the nervous systems, such as learning and memory,

decision making, reward, attention, addiction, depression.

5.04746.0001

LY 367385 Hydrochloride A potent antagonist selective for mGlu1a receptors (IC50

= 8.8 µM and

>100 µM for mGlu1a and mGlu5a, respectively). Negligible effect on

group II and III mGlu receptors. Frequently used in studies assessing the

functional roles of mGlu1a in various brain functions and disorders in

which neural transmissions via mGlu1a receptors are involved.

5.04658.0001

MMPIP A potent negative allosteric modulator highly selective for mGlu7 receptors

(IC50

= 15 nM). No effect on other subtype mGlu receptors. Frequently used

in studies of alcohol and cocaine addition, depression, anxiety and stress

related disorders.

5.04634.0001

MPEP Hydrochloride A non-competitive, highly potent antagonist selective for mGlu5 receptors

(IC50

= 36 nM). Also a positive allosteric modulator of mGlu4and weak

anatagonist of NMDA receptors. Biologically active admitted systematically.

5.04635.0001

Nebivolol Hydrochloride A competitive, highly selective β1-adrenoceptor antagonist (more than

40-fold selective for β1 than β2; Ki1 and β2, respectively). The most

β1-selective chemical of the β-blockers tested so far. Used for treatment of

essential hypertension and highly cardioselective. Induces mild vasodilation

via nitric oxide- and cGMP-depedent pathways. (EC50

= 11.36 µM in renal

arteries).

5.04778.0001

NVP-AAM077 Tetrasodium

Hydrate

A potent antagonist for NMDA receptors. Modestly higher selectivity for

the NR2A than for the NR2B containing NMDA receptors (IC50

= 0.015 and

0.078 µM) but insufficient to discriminate between NR2A and NR2B-

containing NMDA receptors. Widely used for research in synaptic plastic,

learning and memory, and epilepsy.

5.04528.0001

QNZ46 A noncompetitive and voltage-independent antagonist selective for

NR2C/D containing NMDA receptors (IC50

= 6, 3, 229, and >300 µM

for NR2C, NR2D, NR2A, and NR2B, respectively). Inhibition on NR2C/Dcontaining NMDA receptors is glutamate-binding, but not glycine-binding,

dependent.

5.04535.0001

Quisqualic acid A potent agonist highly selective for group I (EC50

≤3 µM) over group II and

III mGlu receptors (EC50

>40 µM) and a potent agonist of AMPA receptors.

Often used for inducing excitotoxic lesions of basal forebrain cholinergic

neurons and spinal neurons.

5.04631.0001

Reboxetine Mesylate A potent and selective inhibitor of noradrenalin uptake (Ki values are 1.1,

129 and >10000 nM for rat NET, SERT and DAT, respectively). Displays

>1000-fold selectivity over α-adrenoceptors, 5-HT, dopamine and

muscarinic ACh receptors. Orally active antidepressant.

5.04120.0001

RS 102895 hydrochloride A selective antagonist for CCR2 receptors (IC50

= 0.36 and 17.8

µM for CCR2b and CCR1 receptors, respectively). Blocks monocyte

chemoattractant protein-1 induced calcium influx and chemotaxis

(IC50

= 32 nM and 1.7 µM respectively). A potential anti-inflammatory

therapeutic agent by inhibiting α1A, α1D and 5-HT1A receptors.

5.04376.0001

Salbutamol Hemisulfate A non-selective β2-adrenergic receptor agonist that displays greater

affinity for β2 receptors (EC50

= 600nM) over B1 receptors. Acts as a

bronchiodilating agent. Clinically used for the treatment of asthma and

chronic obstructive pulmonary disease(COPD).

5.04794.0001































4




Salmeterol Xinafoate A potent, selective, and long-lasting β2-adrenoceptor agonist

(EC50

=5.3 nM) that displays bronchodilator actions. Unlike other β2-

adrenoceptor agonists, Salmeterol binds to exo-site domain of β2-AR

receptors, producing a slow onset of action and prolonged activation.

Clinically used for the treatment of asthma and chronic obstructive

pulmonary disease(COPD).

5.04815.0001

Tamsulosin Hydrochloride A selectiveα1A adrenoceptor antagonist that has preferential selectivity

for the α1A adrenoceptor in the prostate versus the α1B adrenoceptor in

the blood vessels (pKi values are 9.97, 9.64 and 8.86 for α1A, α1B and α1D

subtypes respectively).

5.04793.0001

TCN-201 A negative allosteric modulator selective for NR2A containing NMDA

receptors (pIC50

= 6.8 and <4.3 µM for NR2A and NR2B, respectively). No

effect on NR2B-containing NMDA receptors with concentrations less than

50 mM.

5.04596.0001

γ-Secretase Inhibitor XXV,

FT-9

FT-9, a cell-permeable NSAID-like compound, is a partially selective

γ-Secretase Inhibitor in both microsomes and whole cells. Displays dose-

dependent inhibition of the γ-secretase cleavage of β-Amyloid Precursor

Protein (APP) and APLP2 with IC50

values against APP ICD (intracellular

domain) and APLP2 ICD of ̃ 260 µM, but there is a less potent effect on

γ-secretase processing of APLP1.

500587-10MG







Signaling


Antibodies

Anti-APOL1, clone 1D4 Mouse Hu WB MABS385

Anti-Apolipoprotein L1, clone EPR2906 Rabbit Hu WB, IHC MABS387

Anti-ARG1, clone EPR6671(B) Rabbit Hu, Ms WB MABS388

Anti-Cytochrome P450 17A1,

clone EPR6293

Rabbit Hu WB, IF MABS389

Anti-FABP2, clone EPR7136 Rabbit Hu, Ms, Rt WB MABS390

Anti-FGF23, clone 278-2 Mouse Hu WB MABS460

Anti-G6PD, clone EPR6292 Rabbit Hu WB, IHC MABS391

Anti-GLI1 Rabbit Hu, Rt WB, IHC(P) ABC217

Anti-Gli-1, clone EPR4523 Rabbit Hu WB, IHC MABC678

Anti-LIPE Rabbit Hu WB, IHC, IF ABS432

Anti-Lipin 1 Rabbit Hu WB, IP, IF ABS400

Anti-Monoglyceride Lipase, clone EPR6339 Rabbit Hu WB MABS395

Anti-NFKBIB, clone 7B4 Mouse Hu WB MABS383

Anti-Norrin Rabbit Hu WB, IC ABS440

Anti-Notch 4 Rabbit Hu WB 09-089-I

Anti-phospho LEF (Thr155) Rabbit Hu WB, PIA ABS485

Anti-phospho β Catenin (Ser675) Rabbit Hu WB, PIA ABS481

Anti-phospho-β Catenin (Thr556) Rabbit Hu WB, PIA ABS484

Anti-PIAS2 Rabbit Rt WB, IHC ABS447

Anti-PLAC3 Rabbit Hu WB, IC, IHC ABS444

Anti-PLEKHG7 Rabbit Hu, Ms WB 07-2144

Anti-SHOC2 Rabbit Hu WB, IHC ABS461






























































1

Signaling (continued)



Akt Inhibitor XVI, AT7867 A cell-permeable pyrazolo compound that acts as a potent inhibitor against Akt

(IC50

= 32, 17, and 47 nM against Akt1, Akt2, and Akt3, respectively), PKA (IC50

= 20 nM), and p70S6K (IC50 = 85 nM) in an ATP-competitive manner (K i = 18nM using AKT2), with much reduced potency toward RSK1 (IC

50 >100 nM) and a

panel of 13 other cellular kinases (IC50

>1 µM).

124033-10MG

Bortezomib (MG-341) A cell-permeable dipeptidylboronate compound that selectively inhibits 20S

proteasome β5 ChTL/chymotrypsin- over β1 CL/caspase- and β2 TL/trypsin-like

activity (kinact/Ki = 38,000, 5,700, and <100 M-1s-1, respectively, in human

20S proteasome assays using 10 µM Suc-LLVY-AMC/Cat. No. 539142, 10 µM

Z-LLE-AMC/Cat. No. 539141, or 50 µM Boc-LRR-AMC as substrate).

5.04314.0001

CaCC Blocker IV,

Benzbromarone

A cell-permeable uricosuric agent that, in addition to its known efficacy in gout

treatment, acts as a reversible CaCC blocker and is reported to be more potent

than NFA (Cat. No. 481987) and NPPB (Cat. No. 484100) in suppressing 500

nM Ionomycin- (Cat. Nos. 407950, 407952, and 407953) induced I- influx in

TMEM16A-expressing HEK293 cells (IC50

= 9.97, 140, and 150 µM, respectively).

5.04041.0001

EphA Antagonist A pyrrolyl benzoic acid that effectively competes against ephrin-B3 for EphA4

binding as well as against ephrin-A1/-A2/-A3/-A5 for EphA2 and EphA4 binding

by targeting EphA2/A4 high affinity ephrin-binding pocket in a reversible

manner, exhibiting much reduced or no potency against ephrin-A4 for EphA2/

EphA4 binding, ephrin-A5 for EphA3/A5/A6/A7 binding, ephrin-B2 for B1/B2/

B3/B4/B6 binding, or ephrin-B2 for EphA4 binding.

5.00713.0001

ERK Inhibitor VIII A cell-permeable pyrimidylpyrrole compound that acts as an active site-

targeting, highly potent and selective ERK1/2 inhibitor (KI <2 nM against Erk2;

[ATP] = 65 µM). Orally available in both mice and rats in vivo .

328011-10MG

FAAH Inhibitor III, URB937 A blood brain barrier-impermeable p-hydroxyphenylcarbamate compound that

acts as a potent and irreversible inhibitor of FAAH activity

(IC50

= 26.8 nM rat brain) with excellent selectivity over monoacylglycerol

lipases (IC50

> 100 µM).

341253-5MG

FATP2 Inhibitor, CB-2 A cell-permeable thioxothiazolindine compound that reversibly inhibits

FATP2-mediated long chain fatty acid uptake in Caco-2 and HepG2, but not

non-FATP2-expressing 3T3-L1 adipocytes, cultures (IC50

= 3.99, 7.55, and 231.1

µM, respectively), while exhibiting much reduced potency toward cellular Acsl

(acyl-CoA synthetase) activity (IC50

= 50 µM).

5.00670.0001

Hsp70 Activator, YM-1 A cell-permeable rhodacyanine derivative that stabilizes heat-shock protein

70 in its ADP-bound, substrate-binding conformation via reversible binding to

Hsp70 nucleotide-binding domain (KD = 4.9 µM) in a manner similar to the

physiological Hsp70 co-chaperone Hip.

5.00615.0001








Proteins & Enzymes

ATM, active Kinase Assay 14-933

Di-Ubiquitin (Lys48-linked) Recombinant

Protein

Ubiquitin Assay 17-10408

Di-Ubiquitin (Lys63-linked) Recombinant

Protein

Ubiquitin Assay 17-10388

Furin (Human) Recombinant Enzyme Assay 03-237

Furin (Human) Recombinant Protein Enzyme Assay 03-183

MST4, active Kinase Assay 14-928

Smac/DIABLO Enzyme Assay 23-057

STK25, active Kinase Assay 14-929

XIAP, active Enzyme Assay 23-056











































6



InhibitorSelect™ mTOR

Signaling Regulators Panel

A panel containing 17 potent and selective small molecule regulators that is

useful for the study of mTOR signaling.

475995-1EA

Licofelone, Potassium Salt,

Monohydrate

A cell-permeable, orally active arylpyrrolizine carboxylate that is structurally and

pharmacologically similar to, but less potent than,

MK-886 (Cat. No. 475889). Effectively suppresses cellular Cox pathway PGE2

production by inhibiting COX-1 and mPGES-1, but not COX-2(IC

50 = 0.8, 6, and >30 µM, respectively).

435801-25MG

L-type Calcium Channel

Blocker VIII

A pyrimidinetrione compound that is reported to selectively block CaV1.3 over

CaV1.2 L-type calcium channel activity (85.4% vs. 8.0% inhibition, respectively,

with 5.5 µM inhibitor; IC50

against CaV1.3 = 1.7 µM) using HEK293 transfectants.

208294-25MG

MALT1 Inhibitor II, Mepazine

Acetate

A cell-permeable phenothiazine that that inhibits MALT1, but not caspase-3 or

-8, proteolytic activity (IC50

= 0.83 and 0.42 µM, respectively, against full-length

or paracaspase domain-catalyzed Ac-LRSR-AMC hydrolysis) in a reversible and

noncompetitive manner.

5.00500.0001

mTOR Activator, MHY1485

(Authophagy Inhibitor III)

A cell-permeable triazine derivative that is shown to increase cellular mTOR

Ser2448 and downstream substrate 4E-BP Thr37/46 phosphorylation level in rat

liver Ac2F cells (1 to 2 µM for 1 h), affecting culture viability only at much higher

concentrations (by 20% after 24 h 20 µM treatment).

5.00554.0001

mTOR Inhibitor XI,

ETP-46464

A cell-permeable quinoline-containing heterotricyclic compound that acts as a

potent inhibitor against mTOR, ATR, DNA-PK, PI 3-Kα, and ATM (IC50

= 0.6, 14, 36,

170, and 545 nM, respectively) and preferentially suppresses radiation-inducedcellular ATR activity (>90% at 500 nM) over ATM or DNA-PK activity (IC

50 > 5 µM)

in U2OS cells.

5.00508.0001

mTOR Inhibitor XI, Torin1 A cell-permeable pyridinonequinoline derivative that acts as a highly potent,

ATP-competitive inhibibitor against mTOR and DNA-PK (IC50

= 4.32 and 6.34 nM,

respectively), while inhibiting PI 3-K only at much higher concentrations

(IC50

=171 to 533 nM).

475991-10MG

mTOR Inhibitor XII, Torin2 A Torin1 (Cat. No. 475991) structural analog that acts as a cell-permeable, potent,

ATP-competitive inhibitor against DNA-PK and mTOR (IC50

= 0.5 and 2.81 nM,

respectively).

475992-10MG

Nef Hck Activation Inhibitor,

B9

A cell-permeable diphenylpyrazolo compound that disrupts HIV accessary protein

Nef dimerization (IC50

= 3 µM in HEK293T cells) and prevents Nef-mediated

Src family kinase Hck activation (IC50

= 2.8 µM) without directly affecting the

catalytic activity of c-Src, Hck, Lck, or Lyn.

5.00653.0001

Nur77 LBD Antagonist, TMPA A cell-permeable phenylacetate compound that blocks Nur77 (TR3) LKB1 nuclear-

sequestering function by antagonizing against Nur77-LKB1 interaction via directbinding at the LKB1 LBD/ligand binding domain

(Kd = 0.14 against 5 µM LKB1 binding to 5 µM full-length Nur77).

492910-10MG

p38 MAP Kinase Inhibitor

XIX, Skepinone-L

A cell-permeable phenylamino-dibenzosuberone-diol with a rigid molecular

structure specifically designed to target hydrophobic region I in p38α & p38β, but

not in other kinases with bulky entrance-blocking gatekeeper residues. Shown to

selectively inhibit p38α (IC50

= 5 nM; [ATP] = 100 µM) and p38β (97% inhibition

at1 µM), while exhibiting little affinity toward 400 other kinase constructs.

506174-5MG

PI 3-K Inhibitor XVIII An N-myristoylated cell-permeable PI 3-K p110β-derived Gβγ-binding peptide

(aa 514-537 in p110β C2-helical linker region) that prevents Gβγ-, but not RTK-,

mediated p100β activation both in cell-free assays (1 µM) and in cultures (30 µM

in p110β/p85/Akt/Gβγ-transfected HEK 293E cells) .

5.00499.0001

PKC Activator VII, CGK062 A cell-permeable coumarin-containing compound that is shown to activate PKC

activity and induce cellular PKCα membrane translocation (Effective conc. 12.5 to

50 µM) as well as PKCα-mediated signaling events.

5.04276.0001

Rho Inhibitor II, Y16 A cell-permeable pyrazolidinedione compound that is shown to target RhoGEFDH-PH domain junction with high affinity (Kd = 65 nM) and effetively prevent

RhoGEFs LARG, p115, and PDZ from interacting with RhoA, while displaying

little potency against DBL-RhoA, LBC-RhoA, intersectin-Cdc42, or TrioN-Cdc42

interaction.

5.04043.0001

RyR-Calstabin Interaction

Stabilizer, S107

A cell-permeable, orally active benzothiazepine that effectively blocks leaky

intracellular calcium release by preventing the dissociation/depletion of calstabin

from the ryanodine receptor (RyR) complex due to receptor modifications, such as

oxidation, S-nitrosylation, hyperphosphorylation, or mutation (complete blockage

of 1 mM tetracaine-induced leakage in murine RyR2 S2808D+/+ myocytes at 1

µM).

5.00469.0001

Sphingosine Kinase Inhibitor

IV, CB5468139

A cell-permeable naphthoquinone derivative that acts as an ATP-competitive

inhibitior against sphingosine kinase 1 (IC50

= 2 µM; [sphingosine] = 10 µM &

[ATP] = 100 µM) and several protein kinases, including, but not limited to, TNK2,

AURKB, Met, Syk, AURKC, Fyn, Flt2, PIM2, MST2, CLK1, Tie1, and Fak (IC50

<2 µM).

5.00489.0001

Stearoyl-CoA Desaturase 1

Inhibitor, MF-438

A cell-permeable, thiadiazole-pyridazine compound that acts as an inhibitor of

Stearoyl-CoA Desaturase 1 (SCD1) with an IC50 of 2.3 nM.In vivo , it exhibits an ED

50 of 1-3 mg/kg, p.o., in mouse liver PD assays.

569406-10MG

Signaling (continued)





LEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine,

Mky=Monkey, Can=Canine, Prim=Primate, Por= Porcine,Chimp=Chimpanzee, Rhe Mac=Rhesus Macaque,Ham=Hamster, Chk=ChickenApplications: FC=Flow Cytometry, IC=Immunocytochemistry,IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry(Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation,WB=Western Blotting, ChIP=Chromatin IP,ELISA=Enzyme Immunoassay, DB=Dot Blot, PIA=PeptideInhibition Assay, CS=Cell Sorting, MeDIP=Methylated DNA IP,CC=Cell Culture, BM=Bisulfite Modification

http://www.millipore.com/catalogue/item/475995-1EA?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309



































http://www.millipore.com/catalogue/item/475995-1EA?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309








1

Stem Cell Research


Antibodies

Anti-ACVR1B Rabbit Hu WB ABD133

Anti-AFP, clone 6E6 Mouse Hu WB MABD170

Anti-BMR2, clone 3F6 Mouse Hu, Ms, Rt, Mky WB, IF, IHC MABD171

Anti-FGFR4, clone 4H2B10B2 Mouse Hu WB, IHC MABD120

Anti-FOXA2, clone 7H4B7 Mouse Hu WB, IHC MABD164

Anti-GATA1, clone 4F5 Mouse Hu WB, IC, IHC MABD167

Anti-Human Nuclei, clone 235-1, Biotin

Conjugate

Mouse Hu IC MAB1281B

Anti-ISL1, clone 1H9 Mouse Hu WB, IF, IHC MABD131

Anti-Lefty, clone EPR5444 Rabbit Hu WB MABD186

Anti-LeX Glycan, clone Forse-1 Mouse Rt WB, IHC, IP MABD125

Anti-LIN28, clone 6D1F9 Mouse Hu WB, IF MABD163

Anti-MATN1 Rabbit Hu, Rt WB, IHC, IF ABD131

Anti-NANOS3 Rabbit Hu WB, IHC(P), IF ABD135

Anti-Nestin, clone 10C2, Biotin Conjugate Mouse Hu IC, IHC MAB5326B

Anti-Nestin, clone rat-401, Biotin

Conjugate

Mouse Ms, Rt IC, IHC MAB353B

Anti-Noggin, clone EPR1561 Rabbit Hu WB MABD184

Anti-Nuclei, clone 3E1.3, Alexa Fluor® 488

conjugate

Mouse Hu IC MAB4383A4

Anti-Nuclei, clone 3E1.3, Biotin Conjugate Mouse Hu IC MAB4383B

Anti-Nuclei, clone 3E1.3, Cy3 Conjugate Mouse Hu IC MAB4383C3

Anti-Osteocalcin, clone EPR3690 Rabbit Hu WB MABD182

Anti-PHOX2B Rabbit Hu WB ABD132

Anti-PRDM1, clone 5E7 Mouse Hu WB MABD129

Anti-PRDM16 Rabbit Hu, Rt WB, IHC, IF ABD130

Anti-RUNX1, clone 5A1 Mouse Hu WB, IF MABD169

Anti-SHH, clone 5H4 Mouse Hu, Ms, Mky WB, FC, IHC MABD175

Anti-THAP11, clone 3F3 Mouse Hu WB, IF, IHC, FC MABD172

Anti-WNT1, clone 10C8 Mouse Hu, Ms WB, IF, IHC, FC MABD168

Anti-Wnt-5a, clone 6F2 Mouse Hu, Rt WB, IF, IHC MABD136

Cell Lines

EmbryoMax® Primary Mouse Embryo

Fibroblasts, Neo Resistant, Irradiated,

passage 3

CC PMEF-NX

EmbryoMax® Primary Mouse Embryo

Fibroblasts, Strain CF1, Irradiated, passage 3

CC PMEF-CFX

Human Adipose Mesenchymal Stem Cells Cell

Differentiation,

CC

SCC038

Human iPSC Derived Neural Progenitors Cell

Differentiation,

CC, Neurotoxicity

SCC035

Culture Media & ReagentsESGRO®-2i Supplement Kit (1000x) Stem CC ESG1120

ESGRO®-2i Supplement Kit (1000x) Stem CC ESG1121

Human STEMCCA™/TAT-Cre Bundle E. coli CC SCR545-CRE

PluriSTEM™ Dispase-II Solution Cell Harvesting,

Stem CC

SCM133

PluriSTEM™ Human ES/iPS Cell Medium Embryo Culture,

Stem CC

SCM130

TAT-Cre Recombinase E. coli CC SCR508





LEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine,

Mky=Monkey, Can=Canine, Prim=Primate, Por= Porcine,Chimp=Chimpanzee, Rhe Mac=Rhesus Macaque,Ham=Hamster, Chk=ChickenApplications: FC=Flow Cytometry, IC=Immunocytochemistry,IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry(Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation,WB=Western Blotting, ChIP=Chromatin IP,ELISA=Enzyme Immunoassay, DB=Dot Blot, PIA=PeptideInhibition Assay, CS=Cell Sorting, MeDIP=Methylated DNA IP,CC=Cell Culture, BM=Bisulfite Modification

http://www.millipore.com/catalogue/item/ABD133?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309






http://www.millipore.com/catalogue/item/MAB1281B?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309











http://www.millipore.com/catalogue/item/MAB4383A4?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309


http://www.millipore.com/catalogue/item/MAB4383C3?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309










http://www.millipore.com/catalogue/item/PMEF-NX?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309

http://www.millipore.com/catalogue/item/PMEF-CFX?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309

http://www.millipore.com/catalogue/item/SCC038?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309


http://www.millipore.com/catalogue/item/ESG1120?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309


http://www.millipore.com/catalogue/item/SCR545-CRE?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309

http://www.millipore.com/catalogue/item/SCM133?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309


http://www.millipore.com/catalogue/item/SCR508?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309






http://www.millipore.com/catalogue/item/SCR545-CRE?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309





http://www.millipore.com/catalogue/item/PMEF-CFX?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309

http://www.millipore.com/catalogue/item/PMEF-NX?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309










http://www.millipore.com/catalogue/item/MAB4383C3?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309


http://www.millipore.com/catalogue/item/MAB4383A4?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309






















8

Stem Cell Research (continued)


Kits & Assays

Human Dopaminergic Neurogenesis Kit Cell

Differentiation,

CC, Neurotoxicity

SCR135

Human iPSC Derived Neural Progenitor Kit Cell

Differentiation,

CC, Neurotoxicity

SCR131

Proteins & Enzymes

HumanKine® Cystatin C, Human

Recombinant Xeno-Free

CC GF405

HumanKine® IL-2, Human Recombinant

Xeno-Free

CC GF423

HumanKine® IL-28A, Human Recombinant

Xeno-Free

CC GF425


Xeno-Free

CC GF430


Xeno-Free

CC GF431

HumanKine® M-CSF, Human Recombinant

Xeno-Free

CC GF433

HumanKine® PDGFaa, HumanRecombinant Xeno-Free CC GF436

HumanKine® Pro IGF-II, Human


CC GF437

HumanKine® TGFβ 1, Human Recombinant

Xeno-Free

CC GF439

HumanKine® TGF β 2, Human


CC GF440

HumanKine® TGFβ 3, Human Recombinant

Xeno-Free

CC GF441

HumanKine® VEGF121, Human


CC GF444

Description Details Cat. No.Small Molecules & Inhibitors

InSolution™ Wnt Antagonist

I, IWR-1-endo

A 25 mM (5 mg/489 µL) solution of Wnt Antagonist I, IWR-1-endo

(Cat. No. 681669) in DMSO. A cell-permeable p-imidobenzamidoquinoline,

endo-diastereomer that is shown to inhibit the activity of TNKS1/PARP5a

and TNKS2/PARP5b in in vitro auto-PARsylation assays (IC50

= 131 and

56 nM, respectively) and effectively suppress Wnt-stimulated transcription

activity in L-Wnt-STF-based reporter assays (IC50

= 180 nM).

5.04462.0001

iPSC Induction Enhancer II,

OAC1

A cell-permeable pyrrolopyridinyl-benzamide compound that is reported

to enhance the efficiency of 4F/4TF-induced iPSC generation from MEFs

cultured in either feeder cell-containing ESC media or in feeder-/serum-

free iSF1 media (1 µM OAC1 treatment starts 48 h after viral transduction).

5.00501.0001

ML243 A cell-permeable, biologically stable, 4-ethyl substituted cinnamide analog

that selectively exerts an inhibitory effect on breast cancer stem-like cells

(EC50

= 2 µM for epithelial-to-mesenchymal transition; HMLE_shEcad).

Exhibits ~32-fold greater selectivity over isogenic control cells(EC50

= 64 µM; HMLE cell line expressing shRNA against eGFPcells;

HMLE_sh_eGFP).

5.04599.0001

Porcn Inhibitor II, C59 A cell-permeable pyridinylphenyl-acetamide that is much more potent than

IWP-2 (Cat. No. 681671) in inhibiting MBOAT family member Porcupine-

mediated Wnt palmitoylation (IC50

= 74 pM in STF reporter assays using

Wnt3a-transfected HEK293).

5.00496.0001

Tankyrase1/2 Inhibitor V,

WIKI4

A cell-permeable triazole derivative that acts as a reversible TNKS inhibitor

(IC50

= 15 nM) and selectively inhibits Wnt/β-catenin, but not NF-κB, TGFβ,

or retinoic acid, signaling pathway-dependent transcription activity.

5.00556.0001

iPSC Induction Enhancer II,OAC1(Cat. No. 5.00501.0001)

EMD Millipore recently introduceda compound, OAC1, that enhances

the reprogramming efficiency of 4F

transcription factors (Oct-4, Sox-2, Klf-4,

and c-Myc) by over 20-fold and accelerates

the appearance of induced pluripotent stem

cell-like colonies.

Recently Li and colleagues from the

Beckman Research Institute of City of Hope

showed that OAC1 activated endogenous

Oct-4, Nanog, Sox-2 and also increased

Tet1 mRNA expression*. The iPSC colonies

derived using this compound exhibited

typical embryonic stem cell morphology,

gene expression pattern, and developmental

potential.

* Li, W. et al. 2012. Identification of Oct4-activating

compounds that enhance reprogramming efficiency. Proc.

Natl. Acad. Sci. USA 109, 20853.

PUBLICATION HIGHLIGHTON SMALL MOLECULES:









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1

SmartFlare™ Probes - RNA Detection

Description Cat. No.

SmartFlare™ AFP; Human, Cy3 SF-1120

SmartFlare™ Albumin; Human, Cy3 SF-995

SmartFlare™ AMACR; Human, Cy5 SF-985

SmartFlare™ APOE; Human, Cy5 SF-1133

SmartFlare™ APP; Human, Cy5 SF-1139

SmartFlare™ APRIL; Human, Cy3 SF-411

SmartFlare™ AQP5; Human, Cy5 SF-997

SmartFlare™ ATG9B; Human, Cy3 SF-756

SmartFlare™ BACE1; Human, Cy5 SF-1149

SmartFlare™ BCL11B; Human, Cy5 SF-981

SmartFlare™ BCL2; Human, Cy5 SF-1115

SmartFlare™ BDNF; Rat, Cy5 SF-1159

SmartFlare™ BMP4; Human, Cy5 SF-1067

SmartFlare™ BMP4; Mouse, Cy5 SF-1069

SmartFlare™ BMPR1A; Human, Cy3 SF-1108

SmartFlare™ BMPR2; Human, Cy3 SF-1028

SmartFlare™ BMPR2; Mouse, Cy5 SF-1029

SmartFlare™ BRAF; Human, Cy3 SF-1289

SmartFlare™ CD133; Human, Cy3 SF-958

SmartFlare™ CD31; Mouse, Cy3 SF-1284

SmartFlare™ CD73; Mouse, Cy5 SF-955

SmartFlare™ CDKN2A; Human, Cy5 SF-414

SmartFlare™ Collagen I; Human, Cy3 SF-983

SmartFlare™ Collagen I; Human, Cy5 SF-984

SmartFlare™ Cyclophilin A; Rat, Cy5 SF-1094

SmartFlare™ Dot1L; Mouse, Cy5 SF-998

SmartFlare™ E2F1; Human, Cy3 SF-740

SmartFlare™ EZH2; Mouse, Cy5 SF-1123

SmartFlare™ FABP1; Human, Cy5 SF-986




SmartFlare™ FDXR; Human, Cy3 SF-979SmartFlare™ FDXR; Human, Cy5 SF-1119

SmartFlare™ FGFR1; Human, Cy5 SF-1102

SmartFlare™ Frataxin; Human, Cy3 SF-975

SmartFlare™ Frataxin; Human, Cy5 SF-1116

SmartFlare™ GADD45G; Human, Cy3 SF-1114

SmartFlare™ GATA4; Human, Cy5 SF-1097

SmartFlare™ GATA4; Mouse, Cy5 SF-1092

SmartFlare™ GDF3; Human, Cy5 SF-999

SmartFlare™ Glis1; Human, Cy5 SF-1167

SmartFlare™ GNAS; Human, Cy5 SF-1088

SmartFlare™ HBA1/HBA2; Human, Cy5 SF-992

SmartFlare™ HBB; Human, Cy5 SF-993

SmartFlare™ IAPP; Human, Cy5 SF-1177


SmartFlare™ IAPP; Rat, Cy5 SF-1181

SmartFlare™ IDH2; Human, Cy3 SF-1124

SmartFlare™ IDH2; Human, Cy5 SF-1084

SmartFlare™ IL8; Human, Cy5 SF-763

SmartFlare™ JAK1; Human, Cy3 SF-1288

SmartFlare™ KDR; Human, Cy3 SF-1282

SmartFlare™ KIF5A; Human, Cy5 SF-1183

SmartFlare™ KLF4; Human, Cy3 SF-918

SmartFlare™ KRAS; Human, Cy3 SF-1117

SmartFlare™ KRAS; Human, Cy5 SF-1089

SmartFlare™ KRT10; Human, Cy5 SF-1055

SmartFlare™ LEPR; Human, Cy3 SF-991

SmartFlare™ LRP1; Human, Cy5 SF-1189

SmartFlare™ LRRK2; Human, Cy5 SF-1191

SmartFlare™ MAP2; Human, Cy3 SF-966

SmartFlare™ MAP2; Mouse, Cy3 SF-968

SmartFlare™ MBP; Human, Cy3 SF-1040

SmartFlare™ MBP; Human, Cy5 SF-1039

SmartFlare™ miR-106b-5p; Human, Cy3 SF-1285

SmartFlare™ miR-124-3p; Mouse, Cy3 SF-1095


SmartFlare™ miR-134; Human, Cy3 SF-812


SmartFlare™ miR-142-3p; Human, Cy5 SF-1128





SmartFlare™ miR-193a-5p; Human, Cy5 SF-829



SmartFlare™ miR-203a; Human, Cy5 SF-831

SmartFlare™ miR-24-3p; Human, Cy3 SF-1093SmartFlare™ miR-25-3p; Human, Cy5 SF-454

















SmartFlare™ MPL; Human, Cy5 SF-1090

SmartFlare™ MUC2; Human, Cy5 SF-1112

SmartFlare™ NCAM1; Human, Cy5 SF-1087

SmartFlare™ NF1; Human, Cy3 SF-996

SmartFlare™ NGFR; Human, Cy5 SF-1205

SmartFlare™ NPM1; Human, Cy3 SF-1122

SmartFlare™ Nucleostemin; Human, Cy5 SF-1023

SmartFlare™ P2RY2; Human, Cy5 SF-767

SmartFlare™ PARK2; Mouse, Cy5 SF-1213

SmartFlare™ PARK2; Rat, Cy5 SF-1215

SmartFlare™ PAX6; Human, Cy5 SF-1099

SmartFlare™ PDGFRA; Human, Cy5 SF-1121

SmartFlare™ PDPK1; Human, Cy3 SF-802

SmartFlare™ PDX1; Human, Cy5 SF-1059

SmartFlare™ PICALM; Human, Cy5 SF-1129

SmartFlare™ PICALM; Rat, Cy5 SF-1225

SmartFlare™ PINK1; Human, Cy5 SF-1227

SmartFlare™ PINK1; Rat, Cy5 SF-1231

SmartFlare™ PSEN1; Human, Cy5 SF-1233

SmartFlare™ PSEN2; Human, Cy5 SF-1237

SmartFlare™ PTEN; Human, Cy5 SF-1085

SmartFlare™ RET; Human, Cy5 SF-1286

SmartFlare™ S100A9; Mouse, Cy5 SF-1249

SmartFlare™ S100B; Mouse, Cy3 SF-1104

SmartFlare™ S100B; Mouse, Cy5 SF-1086

SmartFlare™ SIX2; Human, Cy3 SF-973

SmartFlare™ SMAD4; Human, Cy3 SF-1127

SmartFlare™ SMARCB1; Human, Cy5 SF-1118

SmartFlare™ SNCA; Human, Cy5 SF-1253

SmartFlare™ SNCA; Mouse, Cy5 SF-1255SmartFlare™ Somatostatin; Human, Cy5 SF-1063

SmartFlare™ SOX9; Human, Cy5 SF-1011

SmartFlare™ SOX9; Mouse, Cy5 SF-1013

SmartFlare™ STK11; Human, Cy3 SF-1105

SmartFlare™ STK11; Human, Cy5 SF-1083

SmartFlare™ Synaptophysin; Human, Cy5 SF-1047

SmartFlare™ Synaptophysin; Mouse, Cy3 SF-1050

SmartFlare™ TAU; Human, Cy5 SF-1051

SmartFlare™ TDGF1; Mouse, Cy5 SF-1098

SmartFlare™ TLE1; Human, Cy3 SF-934

SmartFlare™ TNFRSF21; Human, Cy5 SF-1261

SmartFlare™ UCHL1; Human, Cy5 SF-1273

SmartFlare™ VPS35; Human, Cy5 SF-1277

Introducing SmartFlare™ RNA Detection Probes!Detect miRNA in LIVE Cells!For more information, including application notes and demo videos, please visit: www.emdmillipore.com/smartflare

VOLUME 4 I 2013

http://www.millipore.com/catalogue/item/SF-1120?cid=BI-XX-BBS-C-EPDF-BIOS-B020-1309










































































































































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Documents

New Products 2013 Version 4