Nikon 50i service manual

R-4758.B

2004 NIKON CORPORATIONC

All rights reserved

Repair Manual

NIKON CORPORATION

Volume-1

MicroscopeECLIPSE 50iECLIPSE 55i

R-4758.B

2

REV. RECORD R-4758 REV. NUMBER:1

PAGE REV.MARK REV. DATE PAGE REV.MARK REV. DATE PAGE REV.MARK REV. DATE

1 B 2005.3.2 46 B 2005.3.2 91 B 2005.3.2 2 47 B 2005.3.2 92 B 2005.3.2 3 B 2005.3.2 48 B 2005.3.2 93 B 2005.3.2 4 B 2005.3.2 49 B 2005.3.2 94 B 2005.3.2 5 B 2005.3.2 50 B 2005.3.2 95 B 2005.3.2 6 B 2005.3.2 51 B 2005.3.2 96 B 2005.3.2 7 B 2005.3.2 52 B 2005.3.2 97 B 2005.3.2 8 B 2005.3.2 53 B 2005.3.2 98 B 2005.3.2 9 B 2005.3.2 54 B 2005.3.2 99 B 2005.3.2

10 B 2005.3.2 55 B 2005.3.2 100 B 2005.3.2 11 B 2005.3.2 56 B 2005.3.2 101 B 2005.3.2 12 B 2005.3.2 57 B 2005.3.2 102 B 2005.3.2 13 B 2005.3.2 58 B 2005.3.2 103 B 2005.3.2 14 B 2005.3.2 59 B 2005.3.2 104 B 2005.3.2 15 B 2005.3.2 60 B 2005.3.2 105 B 2005.3.2 16 B 2005.3.2 61 B 2005.3.2 106 B 2005.3.2 17 B 2005.3.2 62 B 2005.3.2 107 B 2005.3.2 18 B 2005.3.2 63 B 2005.3.2 108 B 2005.3.2 19 B 2005.3.2 64 B 2005.3.2 109 B 2005.3.2 20 B 2005.3.2 65 B 2005.3.2 110 B 2005.3.2 21 B 2005.3.2 66 B 2005.3.2 111 B 2005.3.2 22 B 2005.3.2 67 B 2005.3.2 112 B 2005.3.2 23 B 2005.3.2 68 B 2005.3.2 113 B 2005.3.2 24 B 2005.3.2 69 B 2005.3.2 114 B 2005.3.2 25 B 2005.3.2 70 B 2005.3.2 115 B 2005.3.2 26 B 2005.3.2 71 B 2005.3.2 116 B 2005.3.2 27 B 2005.3.2 72 B 2005.3.2 117 B 2005.3.2 28 B 2005.3.2 73 B 2005.3.2 118 B 2005.3.2 29 B 2005.3.2 74 B 2005.3.2 119 B 2005.3.2 30 B 2005.3.2 75 B 2005.3.2 120 B 2005.3.2 31 B 2005.3.2 76 B 2005.3.2 121 B 2005.3.2 32 B 2005.3.2 77 B 2005.3.2 122 B 2005.3.2 33 B 2005.3.2 78 B 2005.3.2 123 B 2005.3.2 34 B 2005.3.2 79 B 2005.3.2 124 B 2005.3.2 35 B 2005.3.2 80 B 2005.3.2 125 B 2005.3.2 36 B 2005.3.2 81 B 2005.3.2 126 B 2005.3.2 37 B 2005.3.2 82 B 2005.3.2 127 A 38 B 2005.3.2 83 B 2005.3.2 128 A 39 B 2005.3.2 84 B 2005.3.2 129 A 40 B 2005.3.2 85 B 2005.3.2 130 A 41 B 2005.3.2 86 B 2005.3.2 131 A 42 B 2005.3.2 87 B 2005.3.2 132 A 43 B 2005.3.2 88 B 2005.3.2 133 A 44 B 2005.3.2 89 B 2005.3.2 134 A 45 B 2005.3.2 90 B 2005.3.2 135 A

R-4758.B

3

Foreword ..................................................................................................................... P.5 Notes for Repair .......................................................................................................... P.5 Safety Precautions ...................................................................................................... P.5

1. Tools ........................................................................................................................... P.6 1-1. Tools for General Use and Preparations ............................................................... P.6 1-2. Tools for Special Purposes ................................................................................... P.7 1-3. Preparations ......................................................................................................... P.8

2. Specifications................................................................................................................ P.9 2-1. 50i ......................................................................................................................... P.9 2-2. 55i ....................................................................................................................... P.13

3. Optical Systems ........................................................................................................ P.20 3-1 50i ....................................................................................................................... P.20 3-2. 55i ....................................................................................................................... P.21

4. Connection Diagrams ............................................................................................... P.22 4-1. 50i ....................................................................................................................... P.22 4-2. 55i ....................................................................................................................... P.23

5. Disassembling/Assembling/Adjusting ....................................................................... P.24 5-1. Disassembling/Assembling/Adjusting Flow ......................................................... P.24 5-2. Removing Main Unit ........................................................................................... P.26 5-3. Disassembling/Assembling Elevation Block (For 50i/55i) ................................... P.27

5-3-1 Disassembling ................................................................................................ P.27 5-3-2. Assembling .................................................................................................... P.33 5-4. Disassembling/Assembling Condenser Holder Block (For 50i/55i) ..................... P.42 5-4-1. Disassembling ................................................................................................ P.42 5-4-2. Assembling .................................................................................................... P.46 5-5. Disassembling/Assembling Fine/Coarse Focus Block (For 50i/55i) .................... P.52 5-5-1. Disassembling ................................................................................................ P.52 5-5-2. Assembling .................................................................................................... P.59 5-6. Disassembling/Assembling Field Stop Block (For 50i/55i) .................................. P.68 5-6-1. Disassembling ................................................................................................ P.68 5-6-2. Assembling .................................................................................................... P.71 5-7. Disassembling/Assembling ND Filter Block (For 50i) .......................................... P.75 5-7-1. Disassembling ................................................................................................ P.75 5-7-2. Assembling .................................................................................................... P.77

Table of Contents

R-4758.B

4

5-8. Disassembling/Assembling Lighting Block (For 50i) ........................................... P.79 5-8-1. Disassembling .............................................................................................. P.79 5-8-2. Assembling ................................................................................................... P.82 5-9. Disassembling/Assembling Power Supply Assembly (For 50i) ........................... P.85 5-9-1. Disassembling .............................................................................................. P.85 5-9-2. Assembling ................................................................................................... P.87 5-10. Disassembling/Assembling Brightness Control Board (For 50i) .......................... P.89 5-10-1. Disassembling .............................................................................................. P.89 5-10-2. Assembling ................................................................................................... P.91 5-11. Disassembling/Assembling Seesaw Switch (For 50i) ......................................... P.93 5-11-1. Disassembling .............................................................................................. P.93 5-11-2. Assembling ................................................................................................... P.94 5-12. Disassembling/Assembling Color Compensation Filter Unit (For 55i) ................. P.95 5-12-1. Disassembling .............................................................................................. P.95 5-12-2. Assembling ................................................................................................... P.97 5-13. Disassembling/Assembling Diffuser Unit (For 55i) .............................................. P.99 5-13-1. Disassembling .............................................................................................. P.99 5-13-2. Assembling ................................................................................................. P.100 5-14. Disassembling/Assembling Light Source Unit (For 55i) .................................... P.102 5-14-1. Disassembling ............................................................................................ P.102 5-14-2. Assembling ................................................................................................. P.104 5-15. Disassembling/Assembling Switch Board (For 55i) .......................................... P.106 5-15-1. Disassembling ............................................................................................ P.106 5-15-2. Assembling ................................................................................................. P.108 5-16. Disassembling/Assembling Seesaw Switch (For 55i) ........................................P.110 5-16-1. Disassembling .............................................................................................P.110 5-16-2. Assembling ..................................................................................................P.111 5-17. Stage Tilt Adjustment (For 50i/55i) .....................................................................P.112 5-18. Stage Centering Adjustment (For 50i/55i) ..........................................................P.115 5-19. Condenser Holder Tilt Adjustment (For 50i/55i) .................................................P.117 5-20. Condenser Holder Upper Limit Stopper Position Adjustment (For 50i/55i) ....... P.121 5-21. LED Position Adjustment (For 55i) .................................................................... P.122

Table of Contents

Foreword R-4758.B

5

1. This manual is for ECLIPSE 50i and ECLIPSE 55i. Where an item is common for both ECLIPSE 50i and ECLIPSE 55i, (For 50i/55i) is included

in the title. Where an item is exclusive to either ECLIPSE 50i or ECLIPSE 55i, (For 50i) or (For 55i) is

included in the title. For the Stage and Ergonomic Binocular Tube, refer to the ECLIPSE 50i/ECLIPSE 55i

Repair Manual Volume-2. 2. This repair manual has been written to be used as the textbook for the repair training course. This manual is organized so that trainees can understand the mechanical and optical

construction of the microscope by practicing repair procedures from the beginning to the end. 3. The table of contents of this manual includes every item that might require repair and can

efficiently be used as the guide for repair. 4. Unplug the power cable and wear work-gloves before starting work.

Take extra care to avoid injury from sharp edges or projections and electric shock by touching live components.

Notes for Repair: - Use an appropriate screwdriver for the screw head slot. (Especially take care of the (+) slot

head screw). - The plastic parts must be cleaned with a neutral solvent. If an organic solvent (ether, thinner, alcohol, etc.) is used, discoloring or deterioration of the

part might occur. - When cleaning the glass parts, take care to remove dust using a blower, etc. and wipe the

parts with a lens tissue moistened with alcohol or alcohol-ether mixture. Safety Precautions WARNING (For ECLIPSE 50i) The lamp and surrounding areas (including the lamp house) become very hot during and immediately after a period of illumination. - To avoid risk of burns, do not touch the lamp or surrounding areas during or

immediately after a period of illumination. - Make sure the lamp and surrounding areas have cooled sufficiently before attempting

to the replace lamp. - To avoid risk of fire, do not place fabric, paper or highly flammable volatile materials

such as gasoline, petroleum benzine, paint thinner or alcohol near the lamp house while the lamp is lit or during a period of around thirty minutes after the lamp has been turned off.

- To avoid risk of burns, wait at least thirty minutes after the lamp is turned off to give it sufficient time to cool.

Foreword

1. Tools R-4758.B

6

Tool No Name Illustration Remarks

J21084 Hex-key set Set (x6)

T91400 (+) Screwdriver set Set (x4)

T91000 (-) Screwdriver set Set (x6)

T91204 (+) Wood handle driver Diameter 6 mm

T91102 (-) Wood handle screwdriver Width 5.5 mm

T91305 (+) Screwdriver bit (For JCIS screw M2, M2.6) 2.5mm

115mm

T91320 (+) Screwdriver bit holder 8mm

90mm

T92041 Tweezers AA

T92162 Compass spanner A

T92163 Compass spanner B

E719 Handlap (Alcohol container)

1-1. Tools for General Use and Preparations

1. Tools R-4758.B

7


J28005 1 reading collimator

Stage tilt adjustmentCondenser holder tilt

adjustment

J28003 Parallel mirror

Stage tilt adjustmentCondenser holder tilt

adjustment

J25298 Centering tool

Stage centering adjustment

J25481 80i sub stage tool A


J25299 Centering rod


1-2. Tools for Special Purposes

1. Tools R-4758.B

8


J25295 Standard straight tube

LED position adjustment

J25172 Centering telescope


J25260 Ph tool ring


Spring scale

Torque adjustment

1-3. Preparations Alcohol (or alcohol-ether mixture), Lens tissue, Blower, Gasoline (for cleaning), Bamboo stick (for applying lubricant, adhesive), Brass (or tin) foil, Dial gauge For Lubricant and Adhesive: Refer to Repair Parts Lists (50i: KR-7483.A, 55i: KR-7485.A,)

1-2. Tools for Special Purposes/Preparations

2. Specifications R-4758.B

9

I. Overviews 1. Model Number

MBA85010: ECLIPSE 50i, Using halogen lamp as light source L MBA85020: ECLIPSE 50i, Using halogen lamp as light source R

II. Main Items

1. Mounting System - For nosepiece: Square dovetail (compatible with E series) Can be attached and removed using tool. - For eyepiece tube: Round dovetail (compatible with E series) Can be attached and removed using clamp screw.

- For intermediate attachments: Eyepiece tube mounting dovetail and main body top surface (Using M4 screw (4 pcs.))

2. Main Body Top Arm and Stand Blocks

- Height from table top to eyepiece mounting surface: 330 mm - Height from table top to stage top: 199 mm - Max. width: 208 mm - Foot: With vibration-proof damper (4 pcs.)

3. Elevation Block - Focusing system/Stage up-down system: Using roller races - Stage up-down stroke: Upward 2 mm/Downward 28 mm - Sub stage and up-down movement guide: DC integrated type - Stage mounting system: Round dovetail (not compatible with E series) - Condenser lens mounting system: Round dovetail (compatible with E series) - Condenser lens up-down stroke: 27 mm - Condenser lens stroke upper limit position: Adjustable (using tool)

- Condenser lens centering: 3 mm diameter

2-1. 50i


10

4. Coarse/Fine Focus Block - Coarse focus handle: 13.8 mm per rotation (reduction gear ratio: 3) - Fine focus handle: 0.1 mm per rotation (reduction gear ratio: approx. 413) - Minimum reading: 1 micron - Coarse motion handle torque: Adjustable - Refocusing mechanism: With one-touch refocusing mechanism (with

operation lever) When the sub stage is lowered to the lower limit position by pushing the lever down, the sub stage is locked to this position. When the sub stage is lowered 2 mm further from this position by pushing the lever down, the lock mechanism is canceled. The sub stage is returned to the former position. The rotary damper controls the return of the sub stage at a constant speed.

5. Lighting Block

- Composed of collector lens, field lens (f = 89.7), field stop, and filter - Filter: ND8 (Detachable) - Lemon-skin filter (2 pcs.): Built-in (Not detachable) - Diameter of field stop: 1 to 30 mm diameter (design value: 0.8 to 30.5 mm

diameter) - Field stop control: Right-hand side of the microscope base - Filter control: Right-hand side of the microscope base - Additional filter (diameter: 45 mm, thickness: 3 mm): Up to two filters can be

attached tilting 5 degrees above the field lens.

6. Light Source Block - Lamp ratings and type: 6V/30W halogen lamp, PHILIPS 5761

- Average lamp life: 100 hours (listed on product brochure) - Lamp house detachable

2-1. 50i


11

III. Configurations and Functions

1. System Diagram (Described for eyepiece tube, intermediate attachments, nosepiece, stage,

Condenser lens, and polarizer)

2-1. 50i

J-FL 50i55i Epi-Fluorescence Attachment (MBE84000)

C-TE Ergonomic Tube(MBB76500)

Binocular Tube (MBB72100/72105)

Trinocular Tube F(MBB73100/73105)

Trinocular Tube T (MBB73110)

C-TEP DSC Port(MBB76600)

Double Port 0/100(MBB74105)


Drawing Tube (MBB75400)

Teaching Unit S by S type B (MBB75110)

Teaching Unit S by S type AMBB75130

C-SR Right Handle Stage (MBC71600)

C-SR Left Handle Stage (MBC71610)

C-HC1 Specimen Holder 1C (MBC75040)

C-HR2 Specimen Holder 2R (MBC75010)

C-HL2 Specimen Holder 2L (MBC75020) Polarizer with First-order

Red Tint Plate (MBB75380)

Simple Polarizer (MBB75370)

J-CY Cytodiagnostic Unit (MBP89000)

50i Halogen Lamp L(MBA85010)

50i Halogen Lamp R(MBA85020)

:Option

Objective Lens (CFI60)

Intermediate Tube w/Simple Analyzer (MBB75370)

Intermediate Tube w/Analyzer (MBB75360)

Main Teaching UnitMBB75120

Teaching Unit F to F (MBB75100)

C-N Sextuple Nosepiece(MBP71300)

Double Port (MBB74100)

Magnification Module (MBB75500)

Eyelevel Riser (MBB75710)

Condenser (dry) (MBL12000)

Condenser (oil) (MBL12001)

A/A Condenser (MBL71400)

Swing-out Condenser(MBL11300)

Abbe Condenser (MBL71100)

LWD Achromat Condenser (MBL75000)

Achromat Swing-out Condenser 1-100X

(MBL71300)

PH Turret Condenser(MBL73100)


12

2. Restrictions (Following products are not installable.) - Condenser lens D-CUD-E Automatic U condensor (dry) (MBL78500) D-CUD U condensor (dry) (MBL78600) - Intermediate attachments D-FL U-EPI Fluorescence (MBE74100) D-DH-E DIH E (MBD76100) D-DH DIH M (MBD76200) - Stage C-SRR Right handle R stage (MBC71500) C-SLR Left handle R stage (MBC71600) D-S-E Automatic XY stage (MBC76100)

2-1. 50i


13

I. Overviews

1. Model Number MBA85040: ECLIPSE 55i, Using LED as light source L MBA85050: ECLIPSE 55i, Using LED as light source R

II. Main Items

1. Mounting system - For nosepiece: Square dovetail (compatible with E series) Can be attached and removed using tool. - For eyepiece tube: Round dovetail (compatible with E series) Can be attached and removed using clamp screw. - For intermediate attachments: Eyepiece tube mounting dovetail and main

body top surface (Using M4 screw (4 pcs.)) 2. Main Body Top Arm and Stand Blocks

- Height from table top to eyepiece mounting surface: 330 mm - Height from table top to stage top: 199 mm - Max. width: 208 mm - Foot: With vibration-proof damper (4 pcs.)

3. Elevation Block

- Focusing system/Stage up-down system: Using roller races - Stage up-down stroke: Upward 2 mm/Downward 28 mm - Sub stage and up-down movement guide: DC integrated type - Stage mounting system: Round dovetail (not compatible with E series) - Condenser lens mounting system: Round dovetail (compatible with E series) - Condenser lens up-down stroke: 27 mm - Condenser lens stroke upper limit position: Adjustable (using tool)

- Condenser lens centering: 3 mm diameter

2-2. 55i


14

4. Coarse/Fine Focusing Block - Coarse focus handle: 13.8 mm per rotation (reduction gear ratio: 3) - Fine focus handle: 0.1 mm per rotation (reduction gear ratio: approx. 413) - Minimum reading: 1 micron - Coarse motion handle torque: Adjustable - Refocusing function: Refocusing with a single touch of the lever (with

operation lever) When the sub stage is lowered to the lower limit position by pushing the lever

down, the sub stage is locked to this position. When the sub stage is lowered 2 mm further from this position by pushing the lever down, the lock mechanism is canceled. The sub stage is returned to the former position.

The rotary damper controls the return of the sub stage at a constant speed

5. Lighting Block - Composed of collector lens, field lens (f = 89.7), field stop, and filter - Filter: LA60 (Detachable) - Lemon-skin filter (2 pcs.): Built-in (Not detachable) - Diameter of field stop: 1 to 30 mm diameter (design value: 0.8 to 30.5 mm

diameter) - Field stop control: Right-hand side of the microscope base - Filter control: Right-hand side of microscope base - Additional filter (diameter: 45 mm, thickness: 3 mm): Up to two filters can be

attached tilting 5 degrees above the field lens.

6. Light Source Block - White LED array (7 LEDs are used.)

- Lamp house detachable

7. Electric System - Brightness control dial: Rotary system, located on the right-hand side of the

microscope base. - Preset switch: Push-button, located on the right-hand side of the microscope

base. - Lithium-ion battery mounting port: Located on the back of the LED array board. - Input connector: Located on the back. - Power connector for Ergo-View cytodiagnostic unit: Located on the back. - Signal connector for Ergo-View cytodiagnostic unit: Located on the back. - Hand-switch connector for Ergo-View cytodiagnostic unit: Located on the back.

2-2. 55i


15

7-1 Power Input The microscope uses power from an AC adapter or from a battery, as follows. When the power is supplied from an AC adapter, this has priority over any battery.

1. AC adapter input: Option Input voltage: 12 V/1.5 ADC Specified AC adapter: Manufacturer: ILAN ELECTRONICS LTD Model: F1650K - Rated input voltage: 100 to 240 VAC/1.2 A max. (when inputting 115

VAC), 47 to 63 Hz 2. Specified Lithium-ion rechargeable battery: Option Manufacturer: Nikon: Code: EN-EL1 Rating: 7.4 V/720 mAh Specified battery charger: MH-53 or similar

Note: UR-421 (SANYO Electric Co., Ltd) can be used as the equivalent battery charger.

7-2 Power Output (For Ergo-View cytodiagnostic unit) Supply power to Ergo-View cytodiagnostic unit.

- DC 12 V/1 A max.: Current limitation (approx. 1A within 1 sec) - DC 12V/0.5 A max.: Continuous supply

7-3 Battery Run Time 1. When Ergo-View cytodiagnostic unit is not used 4.5 hours minimum: When observed continuously at an LED light source at

maximum light intensity using a new rated-capacity battery 10 hours minimum: When observed continuously at 40% of the maximum current

The following are assumed for this case: - Maximum illuminance using the Ergo-View cytodiagnostic unit with

PlanApo40x and a CB filter attached - 4 lx (eyepiece illuminator reading)

2. When Ergo-View cytodiagnostic unit is used (Reference value) Approx. 3.5 hours: Run time at maximum light intensity assuming that the

magnification of the Ergo-View cytodiagnostic unit is changed 5 times a minute

7-4. Battery Charge Time (Reference value)

Approx. 100 minutes: When a specified battery charger is used

7-5. LOW-Battery Detection LOW-Battery is displayed when the battery voltage lowers to 7.13 +/-0.2 V during operation. The Power LED blinks at a cycle of approx. 5 Hz.

2-2. 55i


16

7-6. Battery Shutdown Detection

When the battery voltage reaches 5.75 +/-0.16 V during operation, the microscope shuts down to prevent the lithium battery from over discharging. It takes 3 to 30 minutes (reference value) from LOW-Battery detection for the microscope to shutdown. The time depends mainly on the number of charge/discharge repetition times, ambient temperature, and LED luminance setting. To reboot the microscope, replace the battery with a charged new battery or switch the power source to the AC adapter.

7-6-1. Replacing Battery Remove the lamp house cover and replace the battery in the battery

pocket with a new one.

7-7. White LEDs Seven white LEDs NSPW510BS (Nichia Corporation) are used

(luminous intensity rank = S or R, color tone rank = b1 or b2, maximum drive current = 22 mA/LED).

7-8. LED Current Variation Range

Approx. 0.05 to 22 mA per LED Current variation dynamic range: Approx. 440 times Total current of seven LEDs is controlled at a constant value. Current variation at maximum light intensity: 5% (reference value)

7-9. Brightness Control

The LEDs light intensity is adjusted with the master brightness control knob on the operation panel. The current variation characteristics (B characteristics) vary with the master brightness control knob characteristics (A characteristics). When the alternate preset switch is pressed, the light intensity adjustment is switched to the preset brightness control which can be adjusted using a (-) screwdriver through the adjustment hole of the operation panel. The preset brightness control knob is visually set to the neutral position (half of maximum light intensity) at the factory prior to shipping.

2-2. 55i


17

7-10. LED Current Control by Varying Magnification of Ergo-View Cytodiagnostic Unit

When the magnification of the Ergo-View cytodiagnostic unit is set to a high value, the LED current increases to approx. 3.2 times, which makes the LED brighter. However, the magnification is limited to the maximum LED current 22 mA. The LED current does not depend on the magnification of the Ergo-View cytodiagnostic unit while the preset switch is ON.

7-11. Life of White LED (Reference data)

According to the reference data provided by the manufacturer, the relation between LED operating time and light intensity is as shown below. The life of white LEDs is calculated assuming that the light intensity at the beginning of test = 100% and operating time = 8 hours/day, 250 days/year

Ambient

temperature Operating

current Operating

time Light

intensity Life of LED

Ta = 25 C IF = 20 mA 3500 hours 70% 1.75 yearsTa = 25 C IF = 20 mA 8000 hours 55% 4 yearsTa = 25 C IF = 10 mA 8000 hours 90% 4 years

7-12. Input/Output Connectors - Input connector for cytodiagnostic unit switch: 3.5 diameter mini-jack Connect the cable of the cytodiagnostic unit hand switch. - Input/output connector for cytodiagnostic unit signal: 3.5 diameter mini-jack Connect the signal cable of the cytodiagnostic unit.

Input/output the hand switch signal and magnification signal. - Power output connector for cytodiagnostic unit: DC jack Connect the cytodiagnostic unit power supply cable.

Note: The shape of this connector differs from the power input connector.

- Power input connector: DC jack Connect the power output plug of AC adapter.

7-13. Indicators - Power ON/OFF indicator LED (Green): Located on the right side

operation panel of the microscope base - Lamp house high temperature caution label - Rated current indication seal

2-2. 55i


18

III. Configurations and Functions

1. System Diagram (Described for eyepiece tube, intermediate attachments, nosepiece, stage,

Condenser lens, and polarizer)

2-2. 55i

J-FL 50i55i Epi-Fluorescence

C-TE Ergonomic Tube(MBB76500)

Binocular Tube (MBB72100/72105)

Trinocular Tube F(MBB73100/73105)

Trinocular Tube T(MBB73110)

C-TEP DSC Port(MBB76600)

Double Port (MBB74100)


Magnification Module


Drawing Tube (MBB75400)

Teaching Unit F to F (MBB75100)

Teaching Unit S by S type B(MBB75110)

Main Teaching UnitMBB75120

Teaching Unit S by S type AMBB75130

C-N Sextuple Nosepiece

C-SR Right Handle Stage (MBC71600)

C-SR Left Handle Stage (MBC71610)

C-HC1 Specimen Holder 1C (MBC75040)

C-HR2 Specimen Holder 2R (MBC75010)

C-HL2 Specimen Holder 2L (MBC75020)

C-ER Eyelevel Riser (MBB75710)


Swing-out Condenser(MBL11300)


LWD Achromat Condenser

Achromat Swing-outCondenser 1-100X

(MBL71300)

J-CY Cytodiagnostic Unit (MBP89000)

55i LED L MBA85040

55iLED R (MBA85050)

:Option

Objective Lens (CFI60)


19

2. Restrictions (Following products are not installable.) - Condenser lens D-CUD-E Automatic U condenser (dry) (MBL78500) D-CUD U condensor (dry) (MBL78600) DF condenser (dry) (MBL12000) DF condenser (oil) (MBL12001) PH condenser (MBL73100) - Intermediate attachments D-FL U-EPI Fluorescence (MBE74100) D-DH-E DIH E (MBD76100) D-DH DIH M (MBD76200) C-ISA simple analyzer (MBB75350) C-IA Analyzer (MBB75360) - Stage C-SRR Right handle R stage (MBC71500) C-SLR Left handle R stage (MBC71600) D-S-E Automatic XY stage (MBC76100) - Others Simple polarizer (MBB75310) Polarizer with first-order red tint plate (MDW10200)

2-2. 55i

3. Optical Systems R-4758.B

20

The following optical system is based on the ECLIPSE 50i. The cemented lens surfaces are omitted from the drawing because the lens direction only is of concern here.

3-1. 50i

6V 30W

Diffuser ND8

Field stop

3. Optical Systems R-4758.B

21

The following optical system is based on the ECLIPSE 55i. The cemented lens surfaces are omitted from the drawing because the lens direction only is of concern here.

3-2. 55i

LED arrays

Lemon skin

Mat surface Evaporated surface

Color compensation filter

Field stop

Evaporatedsurface

Mirror

4. Connection Diagrams R-4758.B

22

4-1. 50i

4. Connection Diagrams R-4758.B

23

4-2. 55i

5. Disassembling/Assembling/Adjusting R-4758.B

24

5-1. Disassembling/Assembling/Adjusting Flow

< ECLIPSE 50i >26

29

85

29

29

30

34

38

38

113

116

118

122

39

40

4073

72

72

69

68 70

74

74

79

80

80

81

81

93

86

85

42

47

75

52

67

89

68

89

Removing Stage/Eyepiece tube/Nosepiece/Condenser lens

Elevation Block

Removing fine/coarse focus block

Removing power supply assembly

Removing elevation block

Removing condenser holder

Disassembling/Assemblingelevation block

Roller race movement adjustment

Installing condenser holder

Installing elevation block

Stage tilt adjustment


Condenser holder tilt adjustment

Condenser holder upperlimit stopper position adjustment

Installing fine/coarse focus block

Elevation block movement checkusing fine/coarse focus handle

Re-focusing mechanismoperation check

Disassembling/Assemblingfine/coarse focus block

Fine/coarse focus block

Fine torque adjustment

Condenser holder block

Disassembling/Assemblingcondenser holder

Knob/rack engaging adjustment

Brightness control board

Removing brightness control dial

Removing field stop block

Disassembling/Assemblingbrightness control board

ND filter block

Disassembling/AssemblingND filter block

Power supply assembly

Removing lamp

Disassembling/Assemblingpower supply assembly

Seesaw switch

Disassembling/Assemblingseesaw switch

Lighting block

Removing lamp

Removing lighting block

Disassembling/Assemblingdiffuser

Disassembling/Assemblingcollector lens

Disassembling/Assemblingcable with lamp socket

Field stop block


Disassembling/Assembling blade

Blade mount position adjustment

Installing field stop block

Diaphragm open-shut dialoperation check

Disassembling mirror/lens

Cleaning adhesive coating surfaceof mirror/lens

Cementing mirror/lens

NOTE: The number at upper right of the frame shows the page of this item.


25

5-1. Disassembling/Assembling/Adjusting Flow

< ECLIPSE 55i >26

29

103

29

29

30

34

38

38

113

116

118

122

39

40

40

52

67

42

47

103

104

105

123

68

69

72

72

73

70

74

74

99

100

101

101

111

107

68

107

95

96

Removing Stage/Eyepiece tube/Nosepiece/Condenser lens

Elevation Block

Removing fine/coarse focus block

Removing control board

Removing elevation block

Removing condenser holder

Disassembling/Assemblingelevation block

Roller race movement adjustment

Installing condenser holder

Installing elevation block

Stage tilt adjustment


Condenser holder tilt adjustment

Condenser holder upperlimit stopper position adjustment

Installing fine/coarse focus block

Elevation block movement checkusing fine/coarse focus handle

Re-focusing mechanismoperation check


Disassembling/Assemblingfine/coarse focus block

Fine torque adjustment


Disassembling/Assemblingcondenser holder

Knob/rack engaging adjustment

Light source Unit

Removing control board

Disassembling/AssemblingLED board

Installing control board


Field stop block


Disassembling/Assembling blade

Blade mount position adjustment

Installing field stop block

Diaphragm open-shut dialoperation check

Disassembling mirror/lens

Cleaning adhesive coating surfaceof mirror/lens

Cementing mirror/lens

Diffuser unit

Removing diffuser unit

Disassembling lens/diffuser

Cleaning adhesive coating surfaceof mirror/diffuser

Installing diffuser unit

Seesaw switch

Disassembling/Assemblingseesaw switch

Color compensation filter unit

Removing color compensationfilter unit

Disassembling/Assemblingcolor filter unit

Switch board

Removing brightness control dial


Disassembling/Assemblingswitch board

NOTE: The number at upper right of the frame shows the page of this item.


26

Before disassembling and assembling the inside mechanisms of the microscope, remove the main unit. This will make it easier to disassemble and assemble the internal parts, and re-adjust the optical system. 1. Eyepiece tube 2. Nosepiece 3. Stage 4. Condenser lens

Loosen the eyepiece clamp screw beneaththe eyepiece tube, and remove theeyepiece tube.

Loosen the clamp screw on the side of thenosepiece, and remove the nosepiece.

Loosen the set screw on the front side ofthe stage using a hex-key, and remove thestage.

Loosen the set screw on the condenser receptacle using a hex-key, and remove the condenser lens.

5-2. Removing Main Unit

Clamp screwEyepiece tube Clamp screw

Nosepiece

StageSet screw Set screw

Condenser lens


27

- Disassembling the elevation block is not usually necessary, but if liquid (water etc.) has dropped into the microscope, it should be disassembled.

- The two roller races (#A3) fixed to the attaching plate must not to be disassembled at the same time. When reassembling one roller race, the other (assembled) roller race is used as a reference for adjustment.

- Some screws are fixed with adhesive. Apply alcohol to melt the adhesive on these screws.

WARNING (For ECLIPSE 50i) - When disassembling the elevation block, remove the lamp cover from the lamp

house. The lamp and surrounding areas (including the lamp house) become very hot

during and immediately after a period of illumination. To avoid risk of burns, do not touch the lamp or surrounding areas during or

immediately after a period of illumination. - To avoid risk of fire, do not place fabric, paper or highly flammable volatile

materials such as gasoline, petroleum benzine, paint thinner or alcohol near the lamp house while the lamp is lit or during a period of around thirty minutes after the lamp has been turned off.

- To avoid risk of burns, wait at least thirty minutes after the lamp is turned off to give it sufficient time to cool.

5-3-1. Disassembling 1. Remove the eyepiece tube. (Refer to Section 5-2.) 2. Remove the objective lens. 3. Remove the condenser lens. (Refer to Section 5-2.) 4. Remove the nosepiece. (Refer to Section 5-2.) 5. Remove the stage. (Refer to Section 5-2.) 6. While pressing the two portions of the lamp

cover (#223) in the arrow directions (a) shown in the photograph, move it in the arrow direction (b) to remove.

5-3-1. Disassembling Elevation Block (For 50i/55i)

Lamp cover(#223)

(a)

(a)

(b)


28

7. In the case of 50i, disconnect the lamp

(#227) to prevent damage. 8. Pull out the hexagonal driver (#248). 9. Remove the four hexagon socket head cap

bolts (M3 x 6) (#254), and remove the plastic cover (#202).

10. Remove the cap (#233). 11. In the case of 50i, remove the power

supply assembly. (Refer to Section 5-9.) In the case of 55i, remove the control board

of the light source unit. (Refer to Section 5-14.)

12. Place the microscope upside down. 13. Remove the five special hexagon bolts

(M3 x 6) (#239), and remove the bottom cover. 14. Remove the two hexagon socket head cap bolts (M3 x 10) (#51), and remove the

plastic cover (#411). 15. Insert a (-) 2 mm screwdriver into the spaces on both sides of the plastic cover

shown in the photograph to release the lock of the claws. [NOTE] When releasing the lock, take care not to break the claws.


Hexagonal driver (#248)

Cap (#233)Plastic cover (#202)

Lamp (#227)

Hexagon socket head

cap bolts (M3 x 6)(#254)

Hexagon socket head cap bolts (M3 x 10) (#51) (-) screwdriver Claws of plastic cover

Bottom coverSpecial hexagon bolts (M3 x 6) (#239)

Special hexagon bolt(M3 x 6) (#239)


Plastic cover (#411)


29

16. Hold the fine/coarse focus block firmly by hand, and remove the two hexagon socket head cap bolts (M3 x 12) (#52).

Remove the fine/coarse focus block and the plastic cover from the microscope base.

17. While holding the elevation block by hand,

place the microscope to its original position. [NOTE] Since the fine/coarse focus block is

removed, the movement of the elevation block is not fixed.

Therefore, when the microscope is placed to its original position, be sure to keep hold of the elevation block.

18. While holding the elevation block firmly by

hand so that it does not drop, remove the six hexagon socket head cap bolts (M4 x 16) (#53) to remove the elevation block.

19. Remove the three hexagon socket head

cap bolts (M3 x 8) (#442), and remove the condenser holder block.


Hexagon socket head cap bolts(M3 x 12) (#52)


Elevation blockHexagon socket head cap bolts (M4 x 16) (#53)

Hexagon socket head cap bolts (M4 x 16) (#53)

Hexagon socket head cap bolts (M3 x 8) (#442)Condenser holder


30

20. Remove the two hexagon socket head cap bolts (M3 x 6) (#441) on the front and two hexagon socket head cap bolts (M3 x 8) (#442) on the rear. Remove the plastic cover (#410).

21. Remove a hexagon socket head cap bolt

(M3 x 12) (#443) of the stroke limitation screw.

22. Remove the two hexagon socket head cap bolts (M3 x 6) (#441), and remove the plate spring (#412) (with the rubber plate).

23. Remove the two hexagon socket head cap bolts (M3 x 8) (#442), and remove the cap (#415) and rotary damper (#414).

24. Tighten a set screw (#452) on the side of

the sub body. 25. Remove the two hexagon socket head cap

bolts (M3 x 6) (#441), and remove the shaft supporter (#407).



Plastic cover (#410) Plastic cover (#410)


Hexagon socket head cap bolt (M3 x 12) (#443) (Stroke limitation screw)

Cap (#415), Rotary damper (#414)

Hexagon socket head cap bolts (M3 x 8)(#442)

Plate spring (#412)Hexagon socket head

cap bolts (M3 x 6) (#441)


Shaft supporter (#407)

Set screw (#452)


31

26. While holding the top surface of the rack

(#406) by finger, loosen the set screw tightened in step 24.

[NOTE] When the set screw is loosened, the rack

will pop out by the power of the two coil springs.

Be sure to hold the top surface of the rack to prevent injury or damage to the surroundings when the rack pops out.

27. Remove the rack, coil spring (#405), coil

spring (#404) and spring guide (#403). 28. Remove the two plate washers from the

hole of the sub body. [NOTE] The two plate washers are only installed

on some microscope. If they are not installed, go to step 29.

Set screw (#452) Rack (#406)

Rack (#406)

Coil spring (#404)

Coil spring (#405)

Spring guide (#403)

Sub body

Plate washers (2 pcs)

Sub body



32

29. Place the elevation block right-hand side down, and fully loosen the four set screws (#447).

30. Remove the four hexagon socket head cap bolts (M3 x 16) (#418), and remove the roller race (#A3) on the left side of the elevation block. Remove the sub body from the elevation Block.

[NOTE] When removing the roller race, the

cylindrical rollers (12 pcs each on up and lower sides) might fall. Be careful not to lose them.

The guide rails (#417) and separators (#408) are also removed at the same time.

31. If there is a rusty or worn section on the cylindrical rollers, separator and/or guide rails (#417), replace it with a new one.

32. If there is a rusty section on the mounting surface of the roller races and attaching plate (#402), scrape it using a scraper.

The disassembly of the elevation block is completed. The disassembled parts should be washed and cleaned.


Sub body

Elevation blockSet screws (#447)

Roller race (#A3) Hexagon socket head cap bolts (M3 x 16) (#418)

Roller race (#A3) Mounting surface

Attaching plate(#402)

Roller race (#A3)


33

5-3-2. Assembling 1. Paste the two guide rails to the two roller

races using lubricant L4010 as a substitute for adhesive.

2. Paste the guide rails to the races on both sides of the sub body using lubricant L4010 in the same way as step 1.

3. Apply lubricant L4010 to the roller race on

the attaching plate assembled in step 1, and place the separator and twelve cylindrical rollers on the roller race.

4. Apply lubricant L4010 to the roller race on the sub body, and place the separator and

twelve cylindrical rollers on the race. 5. Assemble the sub body to the roller race on the attaching plate, and lightly tighten the

roller race with the four hexagon socket head cap bolts (M3 x 16) while pressing it to the sub body.

5-3-2. Assembling Elevation Block (For 50i/55i)

Attaching plate(#402) Guide rails (#417)

Sub body

Roller race (#A3) Roller race (#A3)

Roller race (#A3)

Cylindrical rollers(12 pcs) (#409)

Attaching plate (#402)

Separator (#408)

Sub bodyCylindrical rollers(12 pcs) (#409)

Separator (#408)

Sub body



Roller race (#A3)Sub body


34

6. Move the sub body fully right and left, and adjust the position of the separator so that

the edges of the separator and the roller race coincide. Also adjust the position of the separator on the opposite side in the same way. 7. Move the sub body so that the set screw

on the attaching plate is adjacent to the cylindrical roller.

8. Tighten the set screw close to the cylindrical roller with the following tightening torque using torque driver.

Tightening torque: 1 kg x cm 9. Repeat steps 7 and 8, and tighten the

remaining three set screws in the same way.

10. Tighten the four hexagon socket head cap bolts to fix the roller race.

11. Move the sub body, and check the following:

- The cylindrical roller and separator are still in position.

- The sub body moves smoothly and lightly without slack.

Push the sub body and check, using a spring scale, that 50 to 100g pressure is required to effect movement. If not to meet the specification, repeat steps 7 to 10.

[NOTE] When the four hexagon socket head cap bolts securing the roller race are firmly

tightened, the sub body sometimes loses its movement. In this case, perform steps 7 to 10 again.


Sub body

Edges of the separator andthe roller race are coincided.

Roller race (#A3)


Roller race (#A3)

Set screws (#447)

Sub body

Spring scale

Sub body

Edges of the separator andthe roller race are coincided.

Roller race (#A3)


35

12. Apply adhesive #757 to the threads of the four set screws tightened in steps 8 and 9.

13. After inserting the two plate washers into the 13 mm diameter hole of the sub body, apply lubricant L4010 to the inner surface of the hole.

[NOTE] The two plate washers are only installed

on some microscope. If the plate washers were present during disassembling, be sure to insert them in this step.

14. Put the spring guide into the hole of the sub body, and insert its tip into the center

hole on the spring retainer (#432). [NOTE] Be sure to insert the spring guide tip into the spring retainer hole. 15. Apply a light coating of lubricant L3047 to the whole surfaces of the two kinds of coil

springs. 16. Fit the lubricated coil springs into the hole of the sub body. 17. Install the rack to the sub body while

fitting the two coil springs into the hole of the rack.

[NOTE] Be sure to install the rack in the direction

as shown in the photograph.


Plate washers(2 pcs)

Sub body

Apply lubricant

Sub body

Spring retainer (#432)Tip of the spring guide

Sub body

Spring guide (#403)

Coil spring (#405)

Coil spring (#404)

Sub body

Rack (#406)

Two coil springs and spring guide


36

18. After pushing the rack into the hole so that the edges of the rack and the sub body coincide, lightly tighten a set screw.

19. Install the shaft supporter to the sub body while inserting the spring guide tip into the center hole of the shaft supporter, and tighten it using the two hexagon socket head cap bolts (M3 x 6).

[NOTE] After tightening the bolts, check again that the spring guide tip is inserted into the

spring retainer hole on the opposite side.

(5013_1)

20. Loosen the set screw lightly tightened in

step 18. [NOTE] When the set screw is firmly tightened, the

refocusing function cannot operate.


Set screw (#452) Rack (#406)



Tip of the spring guide

Spring retainer (#432)


Sub body

Loosen this set screw.





37

21. Tighten the hexagon socket head cap bolt (M3 x 12) of the stroke limitation screw. 22. Tighten the plate spring (with rubber plate) using the two hexagon socket head cap

bolts (M3 x 6). When tightening, adjust the position of the plate spring so that the edge of the plate

spring is parallel to the edge of the boss on the attaching plate. 23. Tighten the cap and rotary damper using the two hexagon socket head cap bolts

(M3 x 8).

24. Tighten the plastic cover using the two hexagon socket head cap bolts (M3 x 6) on the front and two hexagon socket head cap bolts (M3 x 8) on the rear.


Plate spring (#412)

Make parallel the edges of the plate spring with the boss on the attaching plate.




Plastic cover (#410) Plastic cover (#410)


Hexagon socket head cap bolts

(M3 x 6) (#441)

Plate spring (#412)


Hexagon socket head cap bolt (M3 x 12) (#443)

(Stroke limitation screw)

Cap (#415) and Rotary damper (#414)


38

25. Install the condenser holder block, and firmly move it in the direction of the arrow. Tighten it with three hexagon socket head cap bolts (M3 x 8).

[NOTE] Be sure to tighten the condenser block

while moving it in the direction of the arrow.

26. While holding the elevation block so that it does not drop, tighten it using the six

hexagon socket head cap bolts (M4 x 16). [NOTE] Be sure to tighten the bolts while pushing the elevation block in the direction of the arrows.

27. While holding the elevation block, place the microscope upside down. [NOTE]

Since the fine/coarse focus block is removed, the elevation block is not fixed. Therefore, when placing the microscope to the original position, hold the elevation block by hand.

28. Perform the following adjustments: - Stage tilt adjustment (Refer to Section 5-17.) - Stage centering adjustment (Refer to Section 5-18.) - Condenser holder tilt adjustment (Refer to Section 5-19.) - Condenser holder upper limit stopper position adjustment (Refer to Section 5-20.)


Condenser holder block Hexagon socket head


Elevation block Hexagon socket head


Hexagon socket head cap bolts (M4 x 16) (#53) Elevation block


39

29. Set the elevator and the coarse/fine focus drive in the following assembly positions:

[Assembling positions] With the main body turned upside down (photo): Elevator: Lowest position Coarse/fine focus drive: Where the limit

screw of the gear contacts the lower limit, as shown in the photograph on the right. (This applies to both right-handle and left-handle designs.)

After these adjustments are completed, place the plastic cover under the fine/coarse focus block, and hold these parts by hand firmly.

While engaging the gear of the fine/coarse focus block with the rack of the elevation block, insert the two pins on the microscope into the two standard holes on the fine/coarse focus block. Tighten the fine/coarse focus block using the two hexagon socket head cap bolts (M3 x 12).

[NOTE] The fine/coarse focus block can be installed to the microscope in both (right and left) directions. Be sure to install it in the same orientation as disassembling.



Fine/coarse focus blockRackGear

Plastic cover Fine/coarse focus block

Standard holes

Pins

Bottom view

Limit screw: To be assembled

at this position


40

[Checking the assembly position of coarse/fine focus drive] When the elevator is at the highest position, its edge should be approximately 1 mm higher than the edge of #402.

Lateral view 30. Put the two claws on right and left sides of the plastic cover (#411) into the two

longitudinal holes of the plastic cover (#410). 31. Tighten the plastic cover using the two hexagon socket head cap bolts (M3 x 10).

32. Tighten the bottom cover using the five special hexagon bolts (M3 x 6).


Longitudinal holesClaws

Special hexagon bolts(M3 x 6) (#239) Bottom cover

Special hexagon bolt(M3 x 6) (#239)

#402 1mm elevator

Edge of #402 Edge of elevator



41

33. Return the microscope to the horizontal position. 34. When the coarse focus knob and the fine focus knob are turned, check that the

elevation block moves smoothly. 35. When the elevation block is pushed down by hand, check that it moves smoothly. Check that the elevation block is locked when the elevation block is fully pushed down,

and that the elevation block is moved upward when the elevation block is pushed down again.

36. In the case of 50i, assemble the power supply assembly. (Refer to Section 5-9.) In the case of 55i, assemble the control board of the light source unit. (Refer to

Section 5-14.) 37. In the case of 55i, perform the LED position adjustment. (Refer to Section 5-21.) 38. Tighten the plastic cover using the four hexagon socket head cap bolts (M3 x 6). 39. Install the cap. 40. In the case of 50i, connect the lamp to the lamp socket. 41. Move the lamp cover in the opposite direction of the arrow, and install it to the plastic

cover. 42. Install the hexagonal driver to the plastic cover.

43 Install the stage. (Refer to Section 5-2.) 44. Install the nosepiece. (Refer to Section 5-2.) 45. Install the condenser lens. (Refer to Section 5-2.) 46. Install the objective lens. 47. Install the eyepiece tube. (Refer to Section 5-2.)

Lamp cover (#223)


Cap (#233)Plastic cover (#202)

Special hexagon bolts(M3 x 6) (#254)

Lamp (#227)

Hexagonal driver (#248)


42

- Disassembling the condenser holder block is not usually necessary, but if liquid (water etc.) has dropped into the microscope, it should be disassembled.

- The two dovetails (#437 and #438) fixed to the condenser holder block must not be disassembled at the same time. When re-assembling the dovetail, the other (assembled) dovetail is used as a reference for assembling.


WARNING (For ECLIPSE 50i) - When disassembling the condenser holder, remove the elevation block from the

microscope base. Be sure to keep the items described in WARNING of the elevation block. (Refer to Section 5-3.)

5-4-1. Disassembling

1. Remove the elevation block from the microscope base according to the steps 1 to 18 of Disassembling in elevation block. (Refer to Section 5-3.)

2. Remove the three hexagon socket head cap bolts (M3 x 8) (#442), and remove the condenser holder block from the elevation block.

3. Loosen the set screw (#446), and pull out

the lever (#439). 4. Loosen the set screw (#446), and pull out

the knob block of the condenser holder block.

5-4-1. Disassembling Condenser Holder Block (For 50i/55i)



Lever (#439)

Set screws(#446)

Condenser holderKnob


43

5. Remove the hexagon socket head cap bolt (#445) of the stroke limitation screw.

6. Slide the condenser holder block to remove it from the block (#422).

7. Remove the two hexagon socket head cap bolts (M3 x 6) (#441), and remove the dovetail (#437 or #438).

[NOTE] When reassembling the dovetail, the other

(assembled) dovetail is used as the reference for adjustment.

8. If there is a rusty section on the mounting surface of the dovetail and block, scrape it using a scraper.

Disassembling male dovetail (#455) and rack (#456)

9. Remove the two hexagon socket head cap bolts (M3 x 8) (#442), and remove the male dovetail (#455) from the condenser holder block.


Hexagon socket head cap bolt (#445)


Condenser holder

Block (#422)

Dovetail (#438) Dovetail (#437)

Hexagon socket head cap bolts (M3 x 6) (#441)Block (#422)

Male Dovetail (#455) Hexagon socket head cap bolts (M3 x 8) (#442)

Condenser holder


44

10. Remove the two special hexagon bolts (M3 x 4) (#457), and remove the rack (#456) from the removed male dovetail.

Disassembling condenser receptacle (#421)

11. Remove the set screw (#444). 12. Fully loosen the two knobs (#A6).

13. Remove the two hexagon socket head cap bolts (M3 x 6) (#441), and remove the spring retainer (#436).

14. Remove the condenser receptacle (#421) from the condenser holder. [NOTE] The plate spring (#435) is removed at the same time.


Rack (#456) Special hexagon bolts(M3 x 4)(#457)

Male Dovetail (#455)

Condenser holder

Knobs (#A6)Set screw (#444)


Condenser holderHexagon socket head


Condenser holder

Condenser receptacle

(#421)

Plate spring (#435)


45

Disassembling knob block

15. Loosen the set screw (#446) fixing the knob (#428).

16. Turn the knob counterclockwise to remove it from the gear (#426).

[NOTE] When removing the knob, the two special

washers (#429), Belleville spring (#430) and special washer (#440) might fall. Be careful not to lose them.

17. Remove the shaft supporter (#424) from the gear.

The disassembly of the condenser holder block is completed. The disassembled parts should be washed and cleaned.


Set screw (#446)

Knob (#428)

Gear (#426)

Remove two special washers (#429), Belleville spring (#430) and special washer (#440).

Gear (#426) Shaft supporter (#424)


46

5-4-2. Assembling When the male dovetail, rack, condenser receptacle, and knob were removed, assemble these parts first, and then assemble the condenser holder according to the steps 1 to 13. The assembling procedures for the male dovetail, rack, condenser receptacle and knob are as follows: - Assembling for male dovetail and rack: steps 14 to 17 - Assembling for condenser receptacle: steps 18 to 22 - Assembling for knob: steps 23 to 28

1. Lightly tighten the removed dovetail (#437 or #438) to the block using the two hexagon socket head cap bolts (M3 x 6).

2. Insert the male dovetail on the condenser holder to the block. While pushing the two dovetails inside, tighten the two hexagon socket head cap bolts.

[NOTE] Move the condenser holder block in the

direction of the arrows, and tighten the hexagon socket head cap bolts.

3. Move the condenser holder block in the

arrow directions, and check that the condenser holder block moves smoothly and lightly without slack.

If the condenser holder block does not move smoothly and lightly without slack, perform step 2 again.

4. Apply lubricant G7826 to the sliding surfaces of the dovetail.

5-4-2. Assembling Condenser Holder Block (For 50i/55i)

Dovetail (#438) Dovetail (#437)


Block (#422)

Male dovetail (#455)

Block (#422) Dovetail (#438)

Dovetail (#437)

Condenser holder


47

5. Tighten the hexagon socket head cap bolt of the stroke limitation screw.

6. Install the knob of the condenser holder to the block, and engage the gear of the knob and the rack of the condenser holder block.

7. Put the rod into the hole of the shaft supporter through the hole of the block. While holding the knob, move the rod in the direction shown in the photograph. The gear should engage firmly with the rack.

Check that the condenser holder moves smoothly by turning the knob.

If the rotation of the knob is heavy, perform the adjustment again using the rod.


Knob (#428) Rod

Shaft supporter (#424)Block (#422)

(#422)

Knob block

Block (#422)

Block (#422)

Condenser holder




48

8. Fix the knob using the set screw. Apply adhesive #757 to the screw threads. 9. Install the lever to the block, and tighten it

using the set screw. Apply adhesive #757 to the screw threads. 10. Install the condenser holder block, and

firmly move it in the direction of the arrow. Tighten it with three hexagon socket head cap bolts (M3 x 8) to the elevation block.

[NOTE] Be sure to install the condenser block

while moving it in the direction of the arrow. 11. While holding the elevation block by hand so that it does not drop, tighten it using the

six hexagon socket head cap bolts (M4 x 16). [NOTE] Be sure to install the elevation block while pushing it in the direction of the arrows.


Knob Condenser holder

Set screws (#446)

Lever (#439)

Condenser holderHexagon socket head



Hexagon socket head cap bolts (M4 x 16) (#43)Elevation block

Elevation block

Knob Condenser holder

Set screws (#446)

Lever (#439)


49

12. After the installation is completed, perform the following adjustments. - Stage tilt adjustment (Refer to Section 5-17.) - Condenser holder tilt adjustment (Refer to Section 5-19.) - Condenser holder upper limit stopper position adjustment (Refer to Section 5-20.) 13. After adjustments are completed, assemble the elevation block according to the steps

29 and later of Assembling in elevation block. (Refer to Section 5-3.)

Assembling male dovetail (#455) and rack (#456) 14. Tighten the rack to the male dovetail using

the two special hexagon bolts (M3 x 4).

15. Lightly tighten the male dovetail to the condenser holder using the two hexagon socket head cap bolts (M3 x 8).


Condenser holder

Male dovetail (#455) Hexagon socket head cap bolts (M3 x 8) (#442)

(#456)

(#455)

Special hexagon bolts (M3 x 4) (#457)

Rack (#456)



50

16. Put the two parallel rods (2 mm diameter) into the grooves of the male dovetail and the

holes of the condenser holder. This sets the relative positions of the male dovetail and the condenser holder.

17. Firmly tighten the two hexagon socket head cap bolts lightly tightened in step 15. After tightening the bolts, pull out the two parallel rods.

Assembling condenser receptacle (#421) 18. Assemble the condenser receptacle and

plate spring to the condenser holder.

19. Apply lubricant G7826 to the specified portion of the spring retainer as shown in the photograph.

20. Install the spring retainer to the condenser holder as shown in the photograph, and tighten it using the two hexagon socket head cap bolts (M3 x 6).


2 mm dia. rodMale dovetail (#455)

Condenser holder

2 mm dia. rod


Condenser holder

Plate spring (#435)

Condenser Receptacle (#421)

Condenser holder

Apply lubricant




51

21. Apply lubricant G6053 to the tip of the set screw, and then thread it to the condenser holder.

22. Screw the two knobs, and adjust the position of the condenser receptacle so that it is mostly located in the center of the condenser holder.

Assembling knob block

23. Apply lubricant L4010 to the specified portion of the gear as shown in the photograph.

24. Insert the gear into the shaft supporter.

25. Apply lubricant L4010 to the end of the shaft supporter.

26. Apply lubricant L4010 on both surfaces of special washer (#440), special washer (#429), Belleville spring (#430) and special washer (#429), and install them to the shaft of the gear in order.

27. Screw the knob to the gear shaft until it stops.

[NOTE] Take care not to pinch the washers

assembled in step 26. 28. Tighten the set screw to fix the knob with the gear. After tightening the set screw, apply adhesive #757 to the screw threads.


Set screw (#444) Knob (#A6)

Condenser holder

Gear (#426) Shaft supporter (#424)

Apply lubricant

Gear (#426)

Knob (#428)

Set screw (#446)

Insert special washer (#440), special washer (#429), Belleville spring (#430) and special washer (#429) in order.


52

5-5-1. Disassembling - Some screws are fixed with adhesive. Apply alcohol to melt the adhesive on these

screws. - The following steps 1) to 8) describe the procedure with the fine/coarse focus block

installed to the microscope. But steps 1) to 8) can also be performed when the fine/coarse focus block has been removed.

1. Remove the fine focus handle cover

(#325). 2. Remove the circular nut (#302), and then

turn the fine motion handle (#324) counterclockwise to remove.

When removing the fine motion handle, grasp the fine motion handle on the opposite side.

3. Remove the Delrin washer (#314),

Belleville spring (#313) and Delrin washer (#314) from the fine motion axle (#323).

5-5-1. Disassembling Fine/Coarse Focus Block (For 50i/55i)

Fine focus handle cover (#325)

Fine motion handle (#324)

Circular nut (#302)

Fine motion axle (#323)

Remove the Delrin washer (#314), Belleville spring (#313) and Delrin washer (#314).


53

4. Remove the fine motion handle (#303) (with the fine motion handle rubber cap (#301))

on the opposite side from the microscope base. The fine motion axle (#323) is removed at the same time. If replacement of the fine motion handle or the fine motion axle is required, replace

these parts after removing the fine motion handle rubber cap (#301) and circular nut (#302).

5. Insert a hex-key into the specified hole

shown in the photograph, and loosen the two set screws (#353) fixing the thrust retainer (#334).

6. Turn the thrust retainer (#334) counterclockwise to remove.

7. Remove the Delrin washer (#315) and

spacer (#312) from the coarse motion axle (#310).


Fine motion handle (#303) (withthe fine motion handle rubber cap)

Hex-key

Thrust retainer (#334)



Circular nut (#302)

Fine motion handlerubber cap (#301)

Remove the Delrin washer (#315) and spacer (#312).

Coarse motion axle (#310)


54

8. Pull out the coarse motion handle (#304)

from the microscope. The coarse motion handle with the gear

case (#307) and coarse motion axle (#310) are removed.

Remove the friction disk (#318) and two wave washers (#316).

[NOTE] When removing the coarse motion handle, the ball bearing (#337) may be detached at the same time. Remove the ball bearing using tweezers etc. Check the rotation of the removed ball bearing using tweezers etc. If replacement of the gear case or the coarse motion axle is required, hold the replacement parts firmly, and turn the other parts counterclockwise to remove.

Perform the following steps when the fine/coarse focus block is removed from the microscope base. 9. Remove the eyepiece tube. (Refer to

Section 5-2.) 10. Remove the objective lens. 11. Remove the condenser lens.

(Refer to Section 5-2.) 12. Remove the stage. (Refer to Section 5-2.) 13. Place the microscope upside down. 14. Remove the five special hexagon bolts

(M3 x 6) (#239), and remove the bottom cover.


Coarse motionhandle (#304)

Remove the friction disk (#318) and two wave washers (#316).

Gear case (#307)


Bottom coverSpecial hexagon bolts (M3 x 6) (#239)

Special hexagon bolts(M3 x 6) (#239)


Plastic cover (#411)


55

15. Remove the two hexagon socket head cap bolts (M3 x 10) (#51), and remove the

plastic cover (#411). 16. Insert a (-) 2 mm screwdriver into the spaces on both sides of the plastic cover shown

in the photograph to release the lock of the claws. [NOTE] When releasing the lock, take care not to break the claws. 17. Hold the fine/coarse focus block firmly by

hand so that it does not drop, and remove the two hexagon socket head cap bolts (M3 x 12) (#52). Remove the fine/coarse focus block and the plastic cover.

18. Remove the two hexagon socket head cap

bolts (M3 x 10) (#352), and remove the gear axle (#327) from the removed fine/coarse focus block.


Hexagon socket head cap bolts (M3 x 10) (#51) (-) screwdriver Claws of plastic cover



Gear axle (#327)


Pinion housing (#305)


56

19. Remove the Teflon washer (#336), gear (#326) and Teflon washer (#336) from the removed gear axle.

Remove the hexagon socket head cap bolt (#354) tightened to the gear.

20. Remove the pinion (#309) from the pinion

housing (#305). 21. Turn the torque adjustment ring (#306)

counterclockwise to remove it from the pinion housing.


Gear (#326)

Gear axle(#327)

Teflon washer(#336)


Pinion housing (#305) Torque adjustment ring (#306)


Pinion (#309)

Teflon washer(#336)

Gear (#326)

Gear axle(#327)

Teflon washer(#336)



57

22. Remove the two kinds of ball bearings

(#337 and #338) and special washer (#339) from the pinion using tweezers etc.

Check the rotation of the removed ball bearings using tweezers etc.

[NOTE] - Special washer sometimes remains in the

pinion housing. When removing, be very careful.

- When removing the coarse motion axle, the ball bearing (#337) is sometimes removed at the same time.

Disassembling gears inside gear case 23. Remove the three hexagon socket head

cap bolts (M3 x 8), and remove the coarse motion handle (#304).

24. Remove the two hexagon socket head cap

bolts (M3 x 8) (#351), and remove the gear retaining plate (#308).


Pinion (#309) Ball bearing (#337)

Ball bearing (#338) andspecial washer (#339)

Three hexagon socket head cap bolts (M3 x 8) (#351)

Coarse motion handle (#304)


Gear retaining plate (#308)


58

25. Remove the three gears (#319, #320 and

#321) from the gear case. The disassembly of the fine/coarse focus block is completed. The disassembled parts should be washed and cleaned.


Gear (#320) Gear (#321)

Gear (#319)

Gear case (#307)


59

5-5-2. Assembling 1. Fit the ball bearings (#337 and #338) into

the pinion. 2. Assemble the pinion into the pinion

housing. 3. Tighten the hexagon socket head cap

bolt (#354) to the gear. 4. Apply lubricant G9110 to both ends of the

gear. 5. Apply lubricant G9110 to the specified

portions of the gear axle as shown in the photograph, and insert the gear axle into the hole of the gear.

5-5-2. Assembling Fine/Coarse Focus Block (For 50i/55i)

Pinion (#309) Ball bearing (#337)

Ball bearing (#338)

Gear axle (#327)

Apply lubricant


Apply lubricant

Gear (#326)

Pinion (#309)



60

6. Apply lubricant G9110 to the two Teflon

washers, and insert them to both ends of the gear axle.

7. Tighten the gear axle to the pinion housing

using the two hexagon socket head cap bolts (M3 x 10).

Although the following steps can be performed where the fine/coarse focus block is removed from the microscope base, the following describes the assembling steps where the fine/coarse focus block is attached to the microscope base.


Teflon washer (#336)

Teflon washer (#336) Gear (#326)

Gear axle (#327)




61

8. Place the plastic cover under the fine/coarse focus block, and hold these parts by

hand firmly. While engaging the gear of the fine/coarse focus block with the rack of the elevation block, insert the two pins on the microscope into the two standard holes on the fine/coarse focus block. Tighten the fine/coarse focus block using the two hexagon socket head cap bolts (M3 x 12).

[NOTE] The fine/coarse focus block can be installed to the microscope in both (right and left)

directions. Be sure to install it in the same orientation as disassembling. 9. Put the two claws on right and left sides of the plastic cover into the two longitudinal

holes of the plastic cover. 10. Tighten the plastic cover using the two hexagon socket head cap bolts (M3 x 10).


Rack

Pins

Longitudinal holesClaws


Plastic cover (#411)Fine/coarse focus block

Standard holes

Gear Hexagon socket head cap bolt (M3 x 12) (#52)


62

11. Tighten the bottom cover using the five

special hexagon bolts (M3 x 6). Assembling gears to gear case 12. Return the microscope to its original

position. 13. Apply lubricant G9110 to both ends of

each gear (#320, #321 and #319), and insert them to the gear case in order.

14. Put the gear retaining plate to the gear

case so that the slightly curved surface of the gear retaining plate faces to the gear, and tighten it using the two hexagon socket head cap bolts (M3 x 8).

[NOTE] When assembling, be sure to install each shaft of the three gears into each hole on the gear retaining plate.

After assembling, check that all gears rotate smoothly.


Gear case (#307)

Gear (#319)

Gear (#321)Gear (#320)

Gear retaining plate (#308)


Bottom cove




63

15. Tighten the coarse motion handle using

the three hexagon socket head cap bolts (M3 x 8).

16. Apply lubricant L3047 to the threads of

the pinion housing and torque adjustment ring, and screw the torque adjustment ring to the pinion housing until it stops.

17. Apply lubricant L3047 to both surfaces of

the two wave washers, and install them to the pinion housing.


Coarse motion handle (#304)


Torque adjustment ring (#306)


Apply lubricant

Wave washers (#316)


64

18. Install the friction disk to the pinion housing.

When installing the friction disk, face its four holes outward, and install its protrusion into the key way on the pinion housing.

19. Install the coarse motion axle (with coarse

motion handle) to the pinion housing. 20. Install the special washer to the coarse

motion axle.


Key way on the pinion housing

Friction disk (#318)

Pinion housing

Coarse motion axle


Special washer (#339)

Protrusion

Four holes

Key way on the pinion housing

Friction disk (#318)


65

21. Install the spacer to the coarse motion axle

into the pinion housing. When installing the spacer, face the grooved surface of the spacer outward as shown in the photograph.

22. Apply lubricant L3047 to both surfaces of

the Delrin washer, and fit it to the coarse motion axle.

23. Apply lubricant L3047 to the surface with V-groove of the thrust retainer. 24. Screw the thrust retainer to the coarse motion axle until it stops. Insert a hex-key into

the hole, and tighten the two set screws. 25. Apply adhesive #757 to the threads of the two set screws.


Spacer (#312)


Grooved surface Outward this side

Delrin washer (#315)



Hex-key


Apply lubricant to the V-grooved surface

Delrin washer (#315)



66

26. Apply lubricant L3047 to the specified portions of the fine motion axle (with the fine

motion handle) as shown in the photograph, and insert it into the gear case. 27. Apply lubricant L3047 to the two Delrin

washers and Belleville spring, and fit them to the fine motion axle.

28. Screw the fine motion handle to the fine

motion axle, and tentatively fix them with the circular nut.


Fine motion axle (with the fine motion handle)

Apply lubricant

Fine motion handle

(#323)

(#314)(#313)(#314)


Circular nut (#302)


Fit the Delrin washer (#314), Belleville spring (#313) and Delrin washer (#314) in order.


67

29. Tentatively install the fine focus handle cover. 30. Wrap the string around the fine focus handle cover as shown in the photograph, and

hook the spring scale on the end of the string. Pull the spring scale in the direction of the arrow.

Check that the value of the spring scale meets the following specification when the fine focus handle starts turning. If not to meet the specification, change the installing position of the fine motion handle screwed in step28, then check again.

Specification: 11.5 to 21 g

(Fine motion torque: 35 to 65 gf x cm) 31. After adjustment is completed, remove the fine focus handle cover once, and then

firmly tighten the circular nut. After tightening, check that the fine motion handle is not slack even if the fine motion handle on the opposite side is turned.

32. Apply adhesive #757 to the threads of the circular nut.

33. Install the fine focus handle cover to the fine motion handle.

34. Install the stage. (Refer to Section 5-2.) 35. Install the objective lens. 36. Install the condenser lens. (Refer to Section 5-2.) 37. Install the eyepiece tube. (Refer to Section 5-2.)


Fine focus handle cover (#325)

Wrap the string around the fine focus handle cover, and hook the springscale on the end of the string. Move the scale in the direction of the arrow. Fine focus handle

cover (#325)


68


5-6-1. Disassembling - If the mirror or the lens attached on the lens holder of the field stop block is

disassembled, perform steps 1 to 9 and steps 13 to 17. 1. Remove the eyepiece tube. (Refer to Section 5-2.) 2. Remove the objective lens. 3. Remove the condenser lens. (Refer to Section 5-2.) 4. Remove the stage. (Refer to Section 5-2.) 5. Place the microscope upside down. 6. Remove the five special hexagon bolts (M3

x 6) (#239), and remove the bottom cover.

7. Remove s

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Nikon 50i service manual