Normal Flora

Microbiotica

Microbiotica

Synder Deep

• CH2O fermentation– Glucose

• Differential– Rate of change– susceptibility

• Selective for lactobacillus– pH = 4.8– Indicator = bromcresol green– Dental caries

BAP

• Divide Plate• Swab throat• Differential

– Sheep Blood– Hemolysis

• Hemolysin exotoxins• Types

– Gamma– Alpha– Beta

Chocolate Agar

• Divide Plate• Swab throat• Differential

– Growth– Oxidase test

• Neisseria

• Lysed RBCs

MSA• Selective• Differential• Divide plate• Swab Skin

– Nose– Face

• Results– Fermentation– acid end products– yellow

• Components– 7.5% NaCl– mannitol– pH indicator = phenol red

MH-T

• Divide plate• Swab skin

– Ears– Palms

• Differential– Diptheroids

• Tellurite– Reduced to tellurium– Black (grey) colonies

Nutrient Agar

• TSA– Not selective– Not differential

• Divide plate

• Swab environment– Moisten swab– Label source

Sabourauds

• Selective– Yeast– Fungus

• +/- Differential• Acidic pH• Divide plate• Swab environment

– Moisten swab– Label source

Urine Culture

• TSA plate• Calibrated loop (0.01ml)• Clean catch sample• Swab plate

– Confluent– Isolation

• Calculate CFU/ml

Normal Flora

Results

Snyder Deep Results• CH2O fermentation

– Glucose

• Differential– Rate of change– Susceptibility for caries

• Selective for lactobacillus– pH = 4.8– Indicator = bromcresol green– Green Yellow– Dental caries

• Slight• Mild• Moderate• Severe

BAP results• Not selective• Differential• Hemolysis

– Hemolysin exotoxins– Types

• Gamma• Alpha• Beta

Chocolate Agar Results

• Differential– Growth– Lysed RBCs– CO2 = capnophiles

• Oxidase Test– Neisseria

Oxidase Test

• Oxidase Reagent– Cytochrome C in ETC– Dark Purple = positive– Neisseria

Oxidase Reaction

MSA Results

• Selective• Differential• Results

– Fermentation– acid end products– yellow

• Components– 7.5% NaCl– Mannitol– pH indicator = phenol red

MH-T Results

• Differential– Diptheroids

• Mueller Hinton– Nutrient agar– Controlled proteins/ions– Diffusion

• Tellurite– Reduced to tellurium– Black (grey) colonies

Sabarouds Results

• Selective– Yeast– Fungus

• +/- Differential• Acidic pH

– 5.5– acidophiles

TSA Environmental Culture

UTI• CFU/ml

• # colonies/0.01ml

• UTI– = > 1x10(5)/ml– Clinical signs– Other urine tests