NOVUS BIOLOGICALS ANTIBODIES FOR EPIGENETICSimages.novusbio.com/design/epi_catalog.pdfNB21-1051 Histone H3 [phospho Thr6] Antibody H3pT6 NB21-1051PEP NB21-1052 Histone H3 [monomethyl

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EPIGENETICS NOVUS BIOLOGICALS ANTIBODIES FOR

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Application KeyChIP - Chromatin Immunoprecipitation

DB - Dot Blot

FACS - Fluorescent Activated Cell Sorting

ICC - Immunocytochemistry

IF - Immunofluorescence

IHC-Fr - Immunohistochemistry Frozen

IHC-P - Immunohistochemistry Paraffin

IP - Immunoprecipitation

WB - Western Blot

Reactivity KeyBv - Bovine

Ce - C. elegans

Ch - Chicken

Dr - Drosophila

Fe - Ferret

Hu - Human

Ma - Mammal

Making Your Success Our GoalNovus is committed to providing researchers with the highest quality antibodies so they may focus on their research. That’s why we’re continually working to provide the most comprehensive data available for our products and its all available to you at www.novusbio.com.

• 114,000+ images• 11,500+ publications• 500+ reviews • 186,000+ products • 31 applications covered• 46 species covered• 42,000+ gene IDs covered

In addition to our primary antibody products, we offer an array of related research tools including immunoprecipitation and labeling kits, stem cell lines and a variety of secondary antibodies, lysates, peptides and proteins.

Novus Quality GuaranteeWe stand behind our products 100%. If you cannot get a prod-uct to work in an application or species stated on our data sheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after trouble-shooting, you can receive a free of charge replacement product or a full refund. Novus’ Quality Guarantee covers 100% of the products we carry. No hassles, no nonsense. It is that simple!

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Mu - Mouse

Po - Porcine

Rt - Rat

Sh - Sheep

Xp - Xenopus

Ye - Yeast

Ze - Zebra Fish

Cover Image: Illustration of strands of DNA coiled around histone cores to form a chromatin fiber.

Table of ContentsIntroduction ..............................1

ChromataChipTM

Immunoprecipitation Kits ...........2

Histones and Modified Histones ...3

Epi-PlusTM Antibodies .................4

Histone Modification Enzymes ... 5-7

Stem Cells ................................8

Support Products .......................9

Posters fromNovus Biologicals ..................10

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Novus’ ChromataChIPTM Kits are the complete solution to help you perform chromatin immunoprecipitation efficiently and accurately in your laboratory. We’ve taken all the guesswork out of ChIP by testing multiple component options to make the best kit on the market.

Each ChromataChIPTM Kit is provided with a postive control antibody from ourEpi-PlusTM modified histone antibody line. Epi-PlusTM antibodies are multi-assay and multi-species validated, including testing by dot blots using modifiedpeptide arrays, western blots, immunocytochemistry and chromatin IP. Blockingpeptides for most Epi-PlusTM antibodies are also avaiblable.

ChromataChIPTM Immunoprecipitation Kits

Kit ComponentsNovus’ ChromataChIPTM Kits come with all the key components you need to perform chromatin IP including a colorful step-by-step protocol pamphlet to walk you through the five main phases of ChIP.

• Positive Control Antibody

• Positive Control Human Primers (RPL30)

• 10X Glycine

• IP Dilution Buffer

• IP Wash Buffer 1, 2, 3 & 4

• IP Elution Buffer

• NaCl solution

• Proteinase K

• Protein A/G Magnetic Beads

• DNA Purification Kit

Product Name Catalog # Unit Size

Basic ChIP kit NBP1-71709 25 immunoprecipitations

Histone H3 [acetyl Lys4] ChIP Kit NBP1-71710 25 immunoprecipitations

Histone H3 [monomethyl Lys9] ChIP Kit NBP1-71713 25 immunoprecipitations

Histone H3 [dimethyl Lys9] ChIP Kit NBP1-71712 25 immunoprecipitations

Histone H3 [trimethyl Lys9] ChIP Kit NBP1-71711 25 immunoprecipitations

Histone H3 [acetyl Lys9/Lys14] ChIP Kit NBP1-71714 25 immunoprecipitations

Contact us about a 2 week free trial.* [email protected]

*Applicable in theUSA only

Stop Wasting Time on Immunoprecipitation

Immunoprecipitation and protein purification are time intensive and disrupt the normal workflow of your lab. Save time and improve consistency with the PrecipitorTM, a machine for high throughput immuno-precipitation and protein purification.

Realize the many advantages of the PrecipitorTM

• IP in less than 30 minutes with no centrifugation or pipetting• Rapid, multi-step automation• 96 well plate format• Multiplex up to 16 samples• Reproducible and consistent results• No external device or PC required• Affordable Price

Learn more at www.novusbio.com/ChromataChIP.html

Visit www.novusbio.com for complete product listings.3

Histones and Modified HistonesHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Histone H1 AntibodyNBP1-19399

Species: HuApplications: IF, IHC-P, WB

Immuno-histochemical analysis of humanlung carcinomatissue usingNBP1-19399.

Histone H4 [Monomethyl Lys20] Antibody NB21-2088

Species: Ce, Hu, Ma, Mu, RtApplications: ChIP, DB, IF, ICC, WB

Western blotanalysisof H4K20me1 in NIH/3T3 histone preps using NB21-2088.

Histone H3 (6F12-H4) [trimethyl Lys9] AntibodyNBP1-30141

Species: Ce, Dr, Hu, Ma, Mu, Rt, YeApplications: ChIP, ELISA, IF, ICC, WB

Immuno-cytochemicalanalysis of HeLa cells using NBP1-30141.

Histone Antibodies

• Histone H1 • Histone H1.1 • Histone H1.2 • Histone H1.3 • Histone H1.4 • Histone H1.5 • Histone H1T

• Histone H2A • Histone H2B • Histone H3 • Histone H3.1 • Histone H3.3 • Histone H3.3B • Histone H4

Recent Citations[Histone H3 Antibody NB500-267] Luo W, Hu H, Chang R, et al. Pyruvate Kinase M2 is a PHD3-Stimulated Coactivator for Hypoxia-Inducible Factor 1.Cell. 2011 May 27;145(5):732-44. [PMID: 21620138][Histone H3 Antibody NB500-267] Karagianni, P., et al. ICBP90, a novel methyl K9 H3 binding protein linking protein ubiquitination with heterochromatin formation. Mol Cell Biol. 2008 Jan;28(2):705-17. Epub 2007 Oct 29.[Histone H3 Antibody NBP1-30141] Tan J, et al. Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells. Genes Dev. 1;21(9):1050-63 (2007).

Histone H3 [Trimethyl Lys4] AntibodyNB21-1023

Species: Ce, Hu, Mu, RtApplications: ChIP, DB, IF, ICC, WB

ChIP: - Two g of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).

Novus offers our Epi-Plus™ antibodies, the most well validated antibodies available for epigenetic research. All Epi-Plus™ antibodies are multi-assay validated, including testing by dot blots using modified peptide arrays, western blots, and chromatin immunoprecipitation (ChIP). Novus Biologicals and 21st Century Biochemicals have collaborated to produce this superior line of Epi-Plus™ antibodies. Learn more at www.novusbio.com/epi-plus.html

Epi-Plus®

Novus has antibodies for hundreds of modified histones. Visit our website for a complete list.


Epi-PlusTM Modified Histone AntibodiesCatalog # Product Name Epigenetic Mark(s) Blocking Peptide Available

NB21-1001 Histone H3 [monomethyl Arg2] Antibody H3R2Me1 NB21-1001PEP

NB21-1002 Histone H3 [asymmetric dimethyl Arg2] Antibody H3R2Me2a NB21-1002PEP

NB21-1021 Histone H3 [monomethyl Lys4] Antibody H3K4Me1 NB21-1021PEP

NB21-1022 Histone H3 [dimethyl Lys4] Antibody H3K4Me2 NB21-1022PEP

NB21-1023 Histone H3 [trimethyl Lys4] Antibody H3K4Me3 NB21-1023PEP

NB21-1024 Histone H3 [acetyl Lys4] Antibody H3K4Ac NB21-1024PEP

NB21-1037 Histone H3 [monomethyl Lys4/phospho Thr6] Antibody H3K4Me1/pT6 NB21-1037PEP

NB21-1038 Histone H3 [dimethyl Lys4/phospho Thr6] Antibody H3K4Me2pT6

NB21-1039 Histone H3 [trimethyl Lys4/ phospho Thr6] Antibody H3K4Me3/pT6 NB21-1039PEP

NB21-1051 Histone H3 [phospho Thr6] Antibody H3pT6 NB21-1051PEP

NB21-1052 Histone H3 [monomethyl Lys9, phospho Thr6] Antibody H3K9Me1pT6

NB21-1053 Histone H3 [dimethyl Lys9/p Thr6] Antibody H3K9Me2pT6

NB21-1054 Histone H3 [trimethyl Lys9/phospho Thr6] Antibody H3K9Me3pT6

NB21-1062 Histone H3 [asymmetric dimethyl Arg8] Antibody H3R8Me2A

NB21-1063 Histone H3 [symmetric dimethyl Arg8] Antibody H3R8Me2s




NB21-1074 Histone H3 [acetyl Lys9] Antibody H3K9Ac

NB21-1081 Histone H3 [acetyl Lys9/Lys14] Antibody H3K9Ac/K14Ac

NB21-1091 Histone H3 [phospho Ser10] Antibody H3pS10

NB21-1092 Histone H3 [phospho Ser10/Thr11] Antibody H3pS10pT11 NB21-1092PEP

NB21-1101 Histone H3 [phospho Thr 11] Antibody H3pT11 NB21-1101PEP

NB21-1132 Histone H3 [asymmetric dimethyl Arg17] Antibody H3R17Me2a





NB21-1181 Histone H3 [monomethyl Lys27] Antibody H3K27Me1

NB21-1182 Histone H3 [dimethyl Lys27] Antibody H3K27Me2

NB21-1183 Histone H3 [trimethyl Lys27] Antibody H3K27Me3


NB21-1202 Histone H3 [symmetric dimethyl Arg2/dimethyl Lys4] Antibody H3R2Me2s/K4Me2 NB21-1202PEP





NB21-2000 Histone H4 [phospho Ser1] Antibody H4pS1

NB21-2011 Histone H4 [monomethyl Arg3] Antibody H4R3Me1

NB21-2024 Histone H4 [acetyl Lys5] Antibody H4L5Ac NB21-2011PEP




More Epi-PlusTM Antibodies coming soon!


DNA Methyltransferase Enzymes (DNMTs)DNA methylation is a major epigenetic modification in the genome of higher eukaryotes. The effects of DNA methylation on cells include transcriptional repression by methylation of promoter regions, formation of compact chromatin structures, X chromosome inactivation and imprinting control. Mutations in genes encoding these DNA methyltransferases cause changes in gene expression and alterations in the patterns of DNA methylation.

DNA Methyltransferase Enzymes(DNMTs) Antibodies

• DMAP1

• DNMT1

• DNMT2

• DNMT3a

• DNMT3b

• DNMT3L

• METTL4

• MGMT

• MTR

Dnmt1 AntibodyNB100-264

Species: HuApplications: ICC, IF, IP, WB

Immuno-cytochemicalanalysis of DNMT1 in nuclei of Hela cellsusing NB100-264.

Dnmt3b Antibody NBP1-85815


Immuno-fluorescent analysis of Dnmt3bshowing positivity in the nucleus using NBP1-85815.

Dnmt3a AntibodyNBP1-85961


Immuno-fluorescent analysis of Dnmt3a showing positivity in nucleus using NBP1-85961.

Customer Reviews

Dnmt1 Antibody (NB100-264)Application: Western BlotSample Tested: HumanSpecies: Human

Dnmt3b Antibody (NB100-266)Application: Western BlotSample Tested: HumanSpecies: Human

Recent Citations[Dnmt1 Antibody NB100-264] Esteve, PO, et al. Direct interaction between DNMT1 and G9a coordinates DNA and histone methylation during replication. Genes Dev. 2006 Nov 15;20(22):3089-103. Epub 2006 Nov 3.[Dnmt1 Antibody NB100-264 and Dnmt3b Antibody NB100-266) Morey Kinney SR, Smiraglia DJ, James SR, et al. Stage-specific alterations of DNA methyltransferase expression, DNA hypermethylation, and DNA hypometh-ylation during prostate cancer progression in the transgenic adenocarcinoma of mouse prostate model. Mol Cancer Res. 2008 Aug;6(8):1365-74. [PMID: 18667590] (Mouse, WB)[PMID 21593185][Dnmt2 Antibody NB200-616] Induction of DNA methylation gene silencing by short interfering RNAs in human cells. Hiroaki Kawasaki, Kazunari Taira. Nature 431: 211-217 (2004).[Dnmt3b Antibody NB100-266] Kim DH, Jung YJ, Lee JE, et al. SIRT1 activation by resveratrol ameliorates cispl-atin-induced renal injury through deacetylation of p53. Am J Physiol Renal Physiol. 2011 Aug;301(2):F427-35.[PMID 21593185][MGMT Antibody NB100-168] Lylo, V. V., Matsevich L. L., Kotsarenko, E. V. et al. V. V. Lyloa, L. L. Matsevicha, E. V. Kotsarenkoa,Activation of Gene Expression of the O6 Methylguanine DNA Transferase Repair Enzyme upon the Influence of EMAP II Cytokine in Human Cells In Vitro. Cytology and Genetics, 2011, Vol. 45, No. 6, pp. 373–378 © Allerton Press, Inc., 2011.

Histone Modification EnzymesIn a variety of DNA-dependent reactions such as transcription, replication, recombination and DNA repair, nucleosome-based chromatin structures are regulated by histone modification enzymes. Histones are subjected to numerous post-translational modifications. There are at least eight distinct types of modifications found on histones. Modification enzymes have been identified for acetylation, methylation, demethylation, phosphorylation, ubiquitination, sumoylation, ADP-ribosylation, deimination, and proline isomerization.

MGMT (MT 23.2) Antibody NB100-168

Species: HuApplications: FACS, IHC-P, WB

Western blot analysis in MCF7 lysate using NB100-168.

Speciiies: HHHu


Histone Modification Enzymes Histone DeacetylasesThese enzymes are a class of enzymes that remove acetyl groups from an -N-acetyl lysine amino acid on a histone. Lysine acetylation is a reversible post-translational modification of proteins and plays a key role in regulating gene expression. Lysine acetylation and deacetylation preferentially targets large macromolecular complexes involved in diverse cellular processes, such as chromatin remodeling, cell cycle, splicing, nuclear transport, and actin nucleation. [PMID: 19608861]

Histone Deacetylase Antibodies

• HDAC1

• HDAC2

• HDAC3

• HDAC4

• HDAC5

• HDAC6

• HDAC7

• HDAC8

• HDAC9

• HDAC10

• HDAC11

• Sir2

• SIRT1

• SIRT2

• SIRT3

• SIRT4

• SIRT5

• SIRT6

• SIRT7

Characteristics of Histone Deacetylases*

SIRT6 Antibody NB100-2522

Species: MuApplications: IHC-P, WB

Immuno-histochemical analysis of human testes tissue using NB100-2522.

HDAC6 Antibody NBP1-78981

Species: HuApplications: IF, ICC

Immuno-cytochemicalanalysis in HeLa cells with FITC (green) using NBP1-78981.

SIRT1 Antibody NBP1-49540

Species: HuApplications: IHC, WB

Immuno-histochemical analysis in human renal cell carcinoma using NBP1-49540.

Customer Review

Sir2.1 Antibody (NB100-1923)Application: IPSample Tested: C. elegansResults: Works well on blot with many different ECL preps.

Class Members Subcellular Localization Tissue Distribution Substrates Binding Partners

I HDAC1 Nucleus Ubiquitous Androgen Receptor, SHP, p53, MyoD, E2F1, STAT3

HDAC2 Nucleus Ubiquitous Glucocorticoid Receptor, YY1, BCL6, STAT3

HDAC3 Nucleus Ubiquitous SHP, YY1, GATA1, RELA, STAT3, MEF2D

HDAC8 Nucleus Ubiquitous EST1B

IIA HDAC4 Nucleus / Cytoplasm Heart, Skeletal Muscle, Brain

GCMA, GATA1, HP1 RFXANK

HDAC5 Nucleus / Cytoplasm Heart, Skeletal Muscle, Bain

GCMA, SMAD7, HP1 REA, Estrogen Receptor

HDAC7 Nucleus / Cytoplasm Heart, Skeletal Muscle, Pancreas, Placenta

PLAG1, PLAG2 HIF1A, BCL6, Endothelin Receptor, ACTN1, ACTN4, Androgen Receptor, Tip60

HDAC9 Nucleus / Cytoplasm Brain, Skeletal Muscle FOXP3

IIB HDAC6 Mostly Cytoplasm Heart, Liver, Kidney, Placenta

-Tubulin, HSP90, SHP, SMAD7

RUNX2

HDAC10 Mostly Cytoplasm Liver, Spleen, Kidney

IV HDAC11 Nucleus / Cytoplasm Brain, Heart, Skeletal Muscle, Kidney

*PMID:17951399


Additional Histone Modification Enzyme Antibodies

• ASH1

• Aurora B

• CARM1

• CKII

• EHMT1

• EHMT2

• FBXL11

• GCn5

• Jarid1A

• Jarid1B

• JMJD1A

• JMJD1B

• JMJD2A

• JMJD2B

• JMJD2C

• JMJD2D

• JMJD3

• JMJD6

• KAT1

• KAT3B/p300

• KDM2B

• KDM6A

• KLSD1

• KMT2a/MLL

• KMT6/EZH2

• MLL2

• MSK2

• NSD1

• PHF8

• PIM1

• PRMT1

• PRMT5

• PRMT6

• SET-2

• SET7/9

• SETD1A

• SETD8

• SETDB1

• SIRT2

• SMYD2

• SUV39H

• SUV420H

Customer Reviews

JMJD2D Antibody (NBP1-03357)Application: Western BlotSample Tested: MDA-MB-435 cell lysatesSpecies: Human

KAT3B/p300 Antibody (NB500-161)Application: ChIPSample Tested: Hypoxic hela cellSpecies: HumanChIP Type: Cross-linking (X-ChIP)

JMJD1A AntibodyNBP1-49601

Species: Hu, MuApplications: IF, ICC, IHC-P, WB

Immuno-histochemicalanalysis of mouse brainusing NBP1-49601.

JMJD2C Antibody NBP1-49600

Species: Hu, MuApplications: IF, ICC, IHC-P, WB

Immuno-histochemicalanalysis of mouse pancreasusing NBP1-49600.

FBXL11 AntibodyNBP1-78305

Species: Hu, MuApplications: ICC, IF, IHC-P

Immuno-histochemicalanalysis in human kidney cancer xenograftusing NBP1-78305.

JARID1B Antibody NBP1-97310

Species: HuApplications: ICC, IF, IHC-P

Immuno-histochemicalanalysis ofhuman breast cancer tissue usingNBP1-97310.

SUV420h1 Antibody NBP1-97313

Species: Hu, MuApplications: ICC, IF, IHC-P

Immuno-fluorescent analysis in A431 cells with FITC (green) usingNBP1-97313.

Recent Citations[Aurora B Antibody NB110-55480] Shen Y-C, Hu F-C, Jeng Y-M, et al. Nuclear Overexpression of Mitotic Regulatory Proteins in Biliary Tract Cancer: Correlation with Clinicopathologic Features Patient Survival. Cancer Epidemiol Biomarkers Prev. 2009;18(2):417-423. [PMID: 19190145][EHMT2 Antibody NB100-40825] Bantscheff M, Hopf C, Savitski MM, et al. Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes. Nat Biotechnol. 2011; [PMID: 21258344][JMJD2B Antibody NB100-74605] Beyer S, Kristensen MM, Jensen KS, et al. The Histone Demethylases JMJD1A JMJD2B Are Transcriptional Targets of Hypoxia-inducible Factor HIF. J Biol Chem 2008;283(52):36542-36552.[KAT3B/p300 Antibody NB500-161] Luo W, Hu H, Chang R, et al. Pyruvate Kinase M2 is a PHD3-Stimulated Coactivator for Hypoxia-Inducible Factor 1.Cell. 2011 May 27;145(5):732-44. [PMID: 21620138][LSD1 Antibody H00023028-M04] Chen W, Obara M, Ishida Y, et al. Characterization of histone lysine-spe-cific demethylase in relation to thyroid hormone-regulated anuran metamorphosis. Dev Growth Differ. 2007 May;49(4):325-34. - [PMID: 17501908]

Histone Modification Enzymes

SETD8 Antibody NBP1-91269

Species: HuApplications: WB

Western blot analysis using NBP1-81513. 1. SETD8 vector 2. empty vector

JMJD6 Antibody (NBP1-71693)Application: Western blotSample Tested: MDA-MB-435 cell lysates Species: Human


Stem CellsStem cells rely heavily on epigenetic signals. As a stem cell develops, chemical tags on the DNA or its surrounding histone proteins switch genes on or off, controlling a cell’s fate. Stem cell markers allow for the identification and isolation of stem cells in different types of tissues and cell populations.

Stem Cell Marker Antibodies

• BMi1

• CD31

• Nanog

• Nestin

• OCT4

• SOX2

• SSEA

• STRO-1

• Tra-1-60(R)

• Tra-1-81

Nanog AntibodyNB100-58842

Species: MuApplications: ICC, IHC-P

Immuno-histochemicalanalysis of mouse testis using NB100-58842.

Nestin (10C2) Antibody NB300-266

Species: HuApplications: FACS, ICC, IF, IHC, IHC-Fr, IHC-P, WB

Immuno-cytochemicalanalysis ofHeLa cells (green) stained usingNB300-266.

OCT4 Antibody NB100-2379

Species: Hu, Bv, Fe, Mu, Mk, PoApplications: FACS, ICC, IF, WB

Immuno-cytochemicalanalysis in NTERA-2 cells detected with Dylight 488using NB100-2379.

SOX2 Antibody NB110-37235

Species: Ch, Hu, Mu, Sh, Ze, XpApplications: FACS, IF, ICC, IHC-P, WB

Immuno-cytochemicalanalysis of NTERA-2 cells using NB110-37235.

Customer Reviews

SOX2 Antibody (NB110-37235)Application: Western BlotSample Tested: Oral cancer cell lineSpecies: HumanResults: Good antibody for testing in Western Blot. Clear signal at 40 kDa

Nanog Antibody (NB100-588)Application: Western BlotSample Tested: HUVEC, MCF7, JURKATSpecies: Human

SSEA3 Antibody (NB100-1832)Application: IHC-PSample Tested: HeLa cellsSpecies: HumanResults: Formalin-fixed, paraffin embedded HeLa cells display immuno-reactivity to SSEA3

DICER Knockout Stem Cell Line (NBP1-96751)Dicer is essential for the processing of small non-coding RNAs including microRNAs and endogenous small interfering RNAs. These cells are a valuable research tool for studying the small non-coding RNAs in embryonic stem cells and the differentiated cells derived from them.

DGCR8 Knockout Stem Cell Line (NBA1-19349)The DGCR8 cells have been genetically altered at the locus of the dgcr8 gene such that no functional DGCR8 protein can be produced, resulting in the global, but specific, loss of micro RNAs.

Recent Citations[DICER knockout stem cell line NBP1-96751 & DGCR8 knockout stem cell line NBA1-19349] Babiarz JE, Ruby JG, Wang Y, Bartel DP, Blelloch R. Mouse ES cells express endogenous shRNAs, siRNAs, and other Microproces-sor-independent, Dicer-dependent small RNAs. Genes Dev. 2008 Oct 15;22(20):2773-85. [PMID: 18923076].[SOX2 Antibody NB110-37235] Domyan ET, Ferretti E, Throckmorton K, et al. Signaling through BMP receptors promotes respiratory identity in the foregut via repression of Sox2. Development. 2011 Mar;138(5):971-981. [PMID: 21303850]

Stem Cell Lines

Bmi1 (LLBmi1-1) Antibody NBP1-96140

Species: Hu, MuApplications: ChIP, ICC, IHC-P, WB

Immuno-histochemicalanalysis of human renal cancer using NBP1-96140.

v6.5 Mouse Embryonic Stem Cell Line (NBP1-4162)The cell line was established from cells derived from the inner cell mass (ICM) of a 3.5 day old mouse embryo from a C57BL/6 X 129/sv cross and has been used successfully in producing germline transmitting chimeras. The v6.5 mouse ES cell lines is the parent strain from which the DGCR8 -/- knockout cell line (NBA1-19349) is derived.

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Support ProductsNovus offers a variety for products for use in conjunction with our Primary Antibodies. All support products are also of the highest quality and are backed by our Novus Guarantee, no hassles, no nonsense.

Secondary Antibodies, Lysates, Peptides, Proteins & RNAIs

Visit www.novusbio.com and select your primary antibody of interest to see all of the related

support products.

AbSelect Antibody Purification Kits

Commercially available antibodies often contain

substances (e.g. BSA, glycine, tris, azide) that interfere in

labeling reactions. The AbSelect Purification Kit quickly removes

these contaminants.

Lightning-Link Antibody Labeling Kits

Lightning-Link is the easiest and quickest method available for making antibody (protein) conjugates, requiring just 30

seconds hands-on time.

Epitope TagsEpitope tagging is a powerful

tool for the detection and purification of expressed

proteins, and Novus Biologicals offers a wide range of epitope tag specific antibodies for a

variety of immunologicalapplications.

Isotype ControlsIdentify any background signal in your FACS experiment with our extensive selection of top

quality isotype control antibodies. Now available for most species, isotypes, and

fluorescent conjugates.

ELISA Kits & Matched Antibody Pairs

Our complete ELISA kits contain all of the components & reagents for a successful ELISA. Plus, it has never been easier to find the right antibody pair for

your sandwich ELISA.

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Novus Explorer This free bioinformatics tool is designed to facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Type in your gene, disease or pathway of interest to begin your exploration. For more information visit www.novusbio.com/explorer.


I N T H E N E W SPosters from Novus Biologicals

Stem Cell Differentiation Pathway

Antibodies for Stem Cell Differentiation Research

EndodermA-fetoprotein; ß-Catenin; CXCR4; N-Cadherin; Sox17; GATA4; GATA6(Progenitor); FoxA2/HNF-3β; HNF-1ß (TCF-2)

HepatocytesA-fetoproteinA-fetoproteinA-fetoprotein; Albumin; GYS1; FAS; Thy-1 (CD90); Integrin ß1 (CD29); CD44H; Integrin a6; HNF-1a; HNF-1ß (TCF-2)Pancreatic cellGlucagon Glut2; Gad65; Gad 67; Insulin, Islet-1; Islet-2; Integrin a6; PDX-1; Pax-6; NeuroD1; Neuropilin-2 Neurogenin-3IntestineHNF-1ß (TCF-2), Cdx2; Msi1

MSC“+”: c-Kit; TAZ; SSEA-1; CD29 (integrin ß1); CD44H; CD90 (Thy-1); Sca-1; Stro-1; Flk-1; CD54; CD56; CD71; TNF receptor I/II; NGFR/p75; Vimentin“-”: CD11a/b; CD19. CD79a; Ly-5; OECAM1; CD34

FibroblastFSP1; P4HBMyoblastActin; Eno-3; Muc18; MyoD; Myf-5; TNNT2; N-Cad; PK-M; Pax3/7; DES; LDH

Myotube (Muscle)

AdipocyteADIPOQ; C/EBP-a; C/EBP-ß; PPARr; Acrp30; GLUT4; DLK1; FABP4

OsteoblastAP; BGLAP; Collagen-1; Fibronectin; RUNX2; IGFBP-3

ChondrocyteCD44; CD151; Collagen II; SOX9; BM-40 (SPARC)

HemangioblastPECA M1; CD34; CD133; T; E-Cad; VE-Cad; VEGF-R2; GATA1; LMO-; Runx1; TIE2

EndotheliumCD14; SSEA1; PECAM1; ICAM-1; NCAM; c-Kit; TNF-R1/II; CXCR4, Sca-1; N-Cad; Nestin; Hif1-a; Stat3; Vimentin; Tie2; TRAIL; Endoglin; E-Selectin; L-Selectin; MUC18; CTLA-4; Integrin ß1/ß2; Ly-5 (CD45)

HSC“+”: BMI-1; PECAM1; HY-1; G-CSF; NOTCH1; NF-YA; WASP; CXCR4; CAMK4; EPCR. V-CAM-1;CD133-CD164; PU.1; FOXO1; Sca-1; N-Cad; RUNX1; CDw338; Flk1“-”: CD2,; CD3; CD4; SSEA1; CD16; CD19; CD20; CD24; CD33; CD36; CD45 (Ly-5); CD48; NCAM (CD56); CD138; GATA3

Skeletal MuscleMuc18; MCAD; Actin; a-Actinin; Desmin; ß-Enolase; MyoD; Myf5;Myogenin, Myosin Heavy/light Chain; Titin; Topponin

Smooth MuscleCaM; Desmin; Vimentin; Smooth muscle myosin

Heart (Cardiogenesis)a-Actinin; GATA4; Myogenin; NF-YA; Cardiac troponin; Cardiacmyosin; CAV2; CAV3; HAND1; HAND2; Nkx2.5; Connexin-43; c-Kit; PECAM1;VCAM1; CD34; TIE2

CommitetedLymphoid ProgenitorCD10; CD34; CD38; Thy-1; c-kit; IL4 Receptor; IL7 Receptor; HLA-DR; TdT

ProerythroblastTransferring receptor; PU.1; Ter119; Endoglin

MyeloblastCD13; SSEA1; G-CSF Receptor; CD33; CD91; GM-CSF Receptor

Reticulocyte Erythrocyte (RBC)

Basophilic myelocyte

Neutrophilicmyelocyte

Eosinophilicmyelocyte

Basophili

Neutrophil

Eosinophil

Pre-NK cell

Thymocytes

Pre B cell

NK lmphoblast

T lymphoblast

B lymphoblast

NK cell

T cell

B cell Plasma cell

MesodermT; BMP2; BMP4; Nodal; PECAM1; CD34; Cryptic; NF-YA; MIXL1; Sca-1; SNA

Embryonic Stem Cell (ESC) or Induced-Pluripotent Stem Cell (iPSC -C-Myc -Sox2 -Oct3/4 -Nanog -Lin28 -Klf-4 -L-Myc

iPSC -AP -E-Cadherin (CD324) -CD49 (integrin a6) -CD133 -C-myv -Cripto -Nanog -Frizzled-5 -Oct-1 -Lin28 -Oct3/4 -Sox02 -SSEA1 (CD15) -SSEA3 -SEEA4 -Smad2/3 -FoxD3 -TRA-1-60 -TRA-1-80

Skin and HairCRABP2; Keratin 15; Fibronectin; Nestin; Integrin ß1; Sca-1; Vimentin

Neural crest stem cellHNK-1; FOXD3; nItegrin-a4; MSX1; Notch1; Neorogenin 3; MASH1; p75NTR

Neural stem cellKi67; ABCG2, Id2; Nestin; Noggin; Sox1; Sox2; Vimentin; Msi1; Msi2; Notch1; CD133; CDw338; Frizzled- 9; SSEA-1; MUC18; CXCR4; Promonin2

Neuronal restricted progenitorß3 tubulin; Nestin; MAP2

Glial restricted progenitorA2B5; FGFR1; FGFR2;FGFR3; FGFR4; Nestin

NeuronN-Cad; CD90; Jagged1; Nestin; Synapsin1; SMN; Synaptophysin; Synaptotagmin; Syntaxin; Tau; TrkB; ChAT; Contactin; GABA-B Receptor; PAX-5; NF-M; Nicastrin; GluR2; GluR4; a-Synuclein; MASH1; TH

Type I AstrocyteRan2; Nestin; FGFR3; GFAP; S100B

Oligodendrocyte progenitorCD44; PGP1; PDGFR-a; RIP; Olig1; Olig2; O1 antigen; O4 antigen

Type II AstrocyteA2B5; GFAP; S100B

OligodendrocyteCD44; PGP1; PDGFR-a; RIP; MBP; Olig1; Olig2; O1 antigen; O4 antigen

NeuronsGliaSchwann cellsSmooth muscle

EctodermNotch1; Nestin; TP63

Sperm or Egg

Primordial germ cellPRDM1, SSEA-1; DAZL; NANOS; PUM2; VASA; PIWIL1/2;Oct3/4; Steel; Stella; VASA

Product Catalog# Host Clonality Species ApplicationsProduct Catalog# Host Clonality Species Applications

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H4

H4

H2B

H2B

H3

H3

H2A

H2A

H2A

H2B

H3

H2B

H4

H4

H2B

H2A

RT K Q A

GG

AP

QA T

AP

TG

GV

H RR P G

VA

LR

EI

RR

YQ

ST

EL

L I R L P F QR

LV

RE

IAQ

DF

D L R FQ

SS/A

A

ALQ

EA

C/S

EA

YL V G L F E

D

NL

CA

IH

A

V

I MP

DIQLA

RR

IR G E

RA

A

T

ST

K

RK

K AA

RK

S

KK

YT

K

K

KT

T

KR

TK

P

AcP

P

P

Ac

Me

Me

Ac Me

Ac

MeP

Ac

Me

P

P

Ac

Me

Ac

Me

P

P

Ac

P

Ac

Me

2

34

6

10

11

14

17

18

23

26

27

28

36

37

4145

56

64

79

80

107

115

118122

Histone H3

GG

G GG L

G

GG

A

RH

R

VL R DD N I Q

T

PA

I

RR

LA

RR

GG V

R I G L I

EE

TR

GV

L

VF

L EN V I R D A V T Y T

HE

A

R

TV

TA M D V V

AL

QG

RT

L Y GF

G

SR

K K

K

K

K K

SY

KK

K

Y

KR

P

Ac

Ac

Me

Ac

Ac

P

P

Me

Ac

Ac

Me

P

Ac

Me1

35 8

12

16

20 31

47

51

5977

79

88

91

92

Histone H4

L

P

Ac

Me

G

CitR8

MeCit

KAc

9

Cit

Ac

A/S31

Ac

Me

87

V/I89M/G90

96

Cit

I

K44

G

Legend

Me

P

Ac Acetylation

Methylation

Phosphorylation

A - AlanineC - CysteineD - Aspartic acidE - Glutamic acidF- PhenylalanineG - GlycineH - Histidine

I - IsoleucineK - LysineL - LeucineM - MethionineN - AsparagineP - ProlineQ - Glutamine

R - ArginineS - SerineT - ThreonineV - ValineW - TryptophanY - Tyrosine

Modifications:

Residues:

Cit Citrullination

Me1

Me2

Me3

Monomethylation

Dimethylation

Trimethylation

Me2

Me1

Me2

Me1

Me3

Me2

Me1

Me3

P

Me2

Me1

Me3

Me2

Me1

Me2

Me1

Me2

Me1

Me2

Me1

Me3

Me2

Me1Ac

Me

Isoform Residue Changes:Histone H3.2 C96 S96Histone H3.3 A31 S31 S87 A87 V89 I89

M90 G90 C96 S96

Ac

Me3

Epi-PlusTM

E

NB110-56924•Beta Catenin

NB600-1161 • Jagged 1

NB500-178 • NUMB

NB110-56923 • Sonic Hedgehog

β-Catenin

TCF/LEFSmad1/5/8

Smad4Smad2/3

Smad4Gli 1/2Gli 3CSLCSL

Gli 3

Gli 1/2/3

Gli 1/2/3

Gli 1/2

Gli 1/2/3

β-CateninSmad1/5/8

Smad4 Smad4Smad2/3

Smad2/3Smad1/5/8

Smad6/7

SKIPSMRTSHARP CIR

CTBPCTBP

MAMLHATs

SKIP BCL9β-Catenin

SOX17

CTBP

CBP CBP

GSK3β

CK 1

Akt

GSK3βPKA

CK1

Fu

NEXT

Notch

Notch

NICD

NICD

CDO

N

LRP5/6

FZD

BMPR2

BMPR1

Type II RType I R

JAG

/Ser

rate

DLL

HhN

HhN

Wnt

CER1

Wnt

BMP

BMP

Activin Nodal

Adam17

Furin

PSEN1/2

HDAC

O-FUT

ICAT

PYGO

FBXW11

SMURF1 SMURF2

LeftyFST

NogginCHRD

WIF

FRP

DKK1

AxinAPC

SMO

Cos2SuFu

SuFu

βTrCp

FBXW11

NCSTN

APH1

Deltex

NU

MB

Fringe

PP2A

NLK

Notch Signaling Hedgehog Signaling Wnt Signaling BMP/Activin/NodalSignaling

Signal sending cell

Ligand degradationor recycling

γ-Secretasecomplex

G G

G

G NotchS2 cleavage

S3 cleavage

S1 cleavage

Off-State

G

DVLDVLNKD

FRAT

Golgi

ER

Nucleus

Expression Expression Expression

Hes 1/3/5Hey1/2MAGCNPase

PreTαMycp21MASH-1

Self-Renewal Cell-Fate Determination

WntGli1CycD1Myc

PatchedIgf2Cyclin EBMPs

MycAxinCyclinD1DKK1

TGF-1CD44WISP1MMP-7

Expression Expression

Txn repressor

Txnactivator

Gli 1/2

β-Catenin

Proteosomal degradation

P

P P

P P

P

P

P

PP

P

P

P

UbUbUbUb

Ub

On-StatePP

ER/Golgi Hedgehog

G

Not

ch

PEN2

Dispatchted

BOC

HhNPatched

Transcription Factors Ligands, Growth Factors, Etc. Membrane Receptors Kinases

Phosphatases Cellular Proteins Signal Transducers Txn Regulators Proteases

DLL

GG

NECD

JAG

/Ser

rate

NBP1-19520 • Smad2 / 3

10


OR

DE

RIN

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Epi Catalog B2

Documents

NOVUS BIOLOGICALS ANTIBODIES FOR EPIGENETICSimages.novusbio.com/design/epi_catalog.pdfNB21-1051 Histone H3 [phospho Thr6] Antibody H3pT6 NB21-1051PEP NB21-1052 Histone H3 [monomethyl