NPGS Genebanksand

NCPN Clean Plant Centers

Joseph PostmanPlant Pathologist & Curator

National Clonal Germplasm RepositoryCorvallis, Oregon

May 2010

U.S. National Plant Germplasm System

(NPGS)

• Scope of Collections

• Pathogen Status (germplasm born pathogens)

• Plant Distribution

(dilution of NCPN efforts?)

• How can NCPN benefit from NPGS

U.S. National Plant Germplasm System

• 441,000 Accessions

• 11,000 Species

Germplasm Resources Information Network (GRIN)

http://www.ars-grin.gov/npgs/

National Clonal Germplasm Repositories

Scope of NPGS Collections: Davis

Genus Accessions Species CountriesActinidia 80 17 4Diospyros 166 5 13Ficus 325 4 17Juglans 591 22 35Olea 153 3 27Pistacia 332 14 25Prunus 1644 94 60Vitis 3306 50 58

Scope of NPGS Collections: GenevaGenus Accessions Species CountriesMalus 6604 55 59Prunus 128 11 13Vitis 1461 27 26

Genus Accessions Species CountriesCitrofortunella 15 3 2Citroncirus 54 2 2Citrus 1323 89 38Fortunella 17 7 3Microcitrus 27 8 5Phoenix 125 8 13Poncirus 61 2 5

Scope of NPGS Collections: Riverside

Scope of NPGS Collections: Corvallis

Genus Accessions Species CountriesCorylus 865 21 42Cydonia 133 2 19Fragaria 1890 43 39Humulus 1582 8 27Pyrus 2242 39 59Ribes 1324 116 41Rubus 2329 198 66Sambucus 205 21 27Vaccinium 1758 69 36

NCGR Corvallis – Collection Summary10,500 total plant and seed accessions

(5100 field plants, 7200 screenhouse plants)

Actinidia2%

Corylus9%

Cydonia1%

Fragaria18%

Humulus5%

Mentha6%Mespilus

0%

Pyrus26%

Ribes10%

Rubus11%

Sambucus1%

Sorbus1%

Vaccinium10%

Clonal Accessions

ex situ clonal collections:

Orchard

Screenhouse

Repository Objectives

VirusDetection

VirusElimination

Heat treatmentMicropropagation

Known Infected Accessions

Genus Infected Clones CultivarsCorylus 0 -Fragaria 205 80Humulus 48 41Pyrus 207 171Ribes 63 54Rubus 67 48Vaccinium* 7 6* phytoplasma

Plant Distribution 2009

521 requests received

5,800 items shipped

Full Pathogen Testing exceeds genebank capabilities

VIRUS AND VIRUS-LIKE DISEASES OF PEARLABORATORY ASSAYSAgent / Disease Serological Assay Molecular AssayACLSV / ring pattern mosaic X XASGV/ pear stem grooving X XASPV/ pear vein yellows XASSVd XPBCVd XPear decline phytoplasma X

GREENHOUSE INDEXINGHerbaceous indicatorsNicotiana occidentalis 37B ACLSV, ASPV (vein yellows)

Woody indicatorsP. veitchii quince sooty ring spot,vein yellowsPyrus communis: Nouveau Poiteau or Jules d'Airolles

pear ring pattern mosaic, (ACLSV),vein yellows (ASPV), (ACLSV),vein yellows (ASPV),

Malus micromalus GMAL 273 ASGVMalus pumila: Virginia Crab ASGV, ASPVCydonia oblonga: C 7/1 Pigwa PBCVdPyrus communis: Fieud 37 ACLSV, PBCVdFIELD INDEXINGP. veitchiiP. communis-Beurre Hardy-Beurre Bosc-Williams-Doyenne du Comice-D. du Comice*-A 20-Jules d` AirollesC. oblonga-C 7/1 PigwaM. pumila: Lord Lambourne, Virginia Crab

Priorities for Genebank Testing?

• Viruses that impact genebank management– Pollen transmitted (Raspberry bushy dwarf,

Blueberry shock)

• Accessions that are highly requested– core accessions– cultivars

Should we not distribute virus infected accessions?

Collaboration between NPGS and NCPN

• Introduction of NPGS accessions to Introduction of NPGS accessions to nurseries is more efficient that foreign nurseries is more efficient that foreign introductions.introductions.

• Include high-priority NPGS accessions Include high-priority NPGS accessions in clean plant testing/clean-up.in clean plant testing/clean-up.

• NPGS can safeguard obsolete NCPN NPGS can safeguard obsolete NCPN accessions.accessions.

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