Onsite Bio Medical Waste Treament

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    Biomedical waste management

    Farid khan

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    Biomedical wastes are treated onsiteutilizing autoclaves

    Autoclaving provides an economical process of killingpathogenic microorganisms through saturation withmoist heat (steam) under pressure

    This process makes the microorganisms nonviable bydestroying (denaturing) essential proteins andstructures

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    Waste Handling Continued

    Bags should be left to cool forseveral minutes beforeremoving from autoclave

    Once cool, securely closebiohazard bag and place intoopaque black bag which haslarge white trash bin assecondary containment

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    Autoclave Use Log

    Record

    date wasteis treated

    Give a

    description

    of waste

    treated

    Record

    weight of

    material

    in pounds

    Always record

    conditions for the

    treatment of Waste

    Record duration

    waste is treated

    and the cycle

    number

    Recordname of

    the person

    running

    the cycle

    Record

    initials of

    person

    running

    cycle

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    Quality Control

    Methods for Quality Control: Autoclave temperature tape (only tells desired

    temperature reached not time or pressure)

    Parameter monitoring (pressure, time and temperature)

    Record every load on autoclave use log Perform Efficacy monitoring

    0 - 100 lbs/month - monthly

    100 - 200 lbs/month - biweekly

    >200 lbs/month - weekly

    Routine parameter monitoring maybe substituted for

    biological monitoring for autoclaves which have a

    continuous readout and record of operating parameters

    (example: autoclave with receipt tape style printout)

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    Parameter Monitoring

    Parameter monitoring is necessaryto ensure efficacy of autoclave

    Parameter can be performed byusing Verify integrator strips

    Autoclaves that use pinwheel plotsrequire biological monitoring withVerify biological monitoring vialscontaining live GeoBacillus

    Stearothermophilus

    Verify Self Contained Biological

    Indications

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    Autoclave Parameter Log

    Staple the Verify

    indicatorstrip from cyclehere.

    Print the name

    and initials of

    person running

    the cycle.

    Record

    the date

    Indicator

    Strip was

    run

    Please

    record the

    temperature,

    pressure,

    duration, and

    the cyclenumber

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    Autoclave Rooms are BSL-2 Rooms

    It is important to rememberthat all autoclave rooms areBSL-2 (biosafety level 2) rooms

    Indicates a biohazard is

    present such as Hepatitis Bvirus, HIV, the salmonellae,and Toxoplasma spp. Alsoincludes human-derived blood,body fluids, tissues, or primaryhuman cell lines where thepresence of an infectious agentmay be unknown

    Indicates that NO FOOD /

    DRINKS or cosmeticsareallowed in the room

    Typical Door Posting

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    Personal Protective Equipment

    When selecting PPE consider the highest level hazard present,the source of the hazard, and the potential forsimultaneous exposures.

    Face protection Goggles or safety glasses with side shields

    Clothing Lab coats, scrub suits, gowns (long pants only, no open toed

    shoes) Clothing should be replaced immediately when contaminated

    Gloves Verify gloves are compatible with your specific application,

    process and materials before using Gloves should be replaced immediately if torn Gloves should not be worn outside the lab area Always wear heat resistant gloves and keep your face away

    from the door when loading and unloading the autoclave

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    Employee Safety Practices

    Avoid compressing bags which may create aerosols

    Biological waste should be stored in rigid leak proofcontainers such as biohazard boxes, bins, or secondarycontainment pans

    Waste should be stored inside the autoclave room andnot in the hallways

    Biological waste containing hazardous chemicals

    (flammables, chemotherapeutic agents) or Sharpsshould not be autoclaved. Call EH&S at 713-500-5837 for collection of these items

    Biological Waste should not be left for someone elseto autoclave

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    Good Employee Work Practices

    Be a conscientious worker Keep work areas clean and free of hazards

    Use Standard Precautions

    Treat all human blood and body fluids as ifknown to be infectious

    No eating, drinking, or applying cosmetics in the workarea

    Frequent hand washing

    Decontaminate work surfaces

    decontaminate surfaces daily

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    Common Biomedical Waste AutoclaveDeficiencies

    EH&S performs semiannual surveys ofautoclaves utilized for biomedical wastetreatment

    Common deficiencies include: Food or drink consumed or stored in

    autoclave area Autoclave log incomplete Sterility monitoring not performed Training records incomplete Minors in the work place

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    In Summary

    Autoclave Use Guidelines to follow:

    3. Place sterilizing indicator tape on the autoclave bag. Make sure bags

    designated for autoclaving are used

    2. Place waste inside autoclave. Use secondary containment (pan) under the bag.

    If waste does not contain any moisture, put cup of water inside bag for

    steam generation

    3. Autoclave according to manufacturers instructions for a minimum 30 minutes,

    at a minimum operating temperature and pressure of 250 deg. F and 15 psig

    4. When cycle is finished, inspect sterilizing tape, and visually check bags to

    ensure autoclave was working correctly5. Place waste in black opaque bags. Seal bag with tape or tie wrap

    6. Place waste in trash cans labeled Autoclaved Waste Only Log activities in the autoclave usage log Perform routine efficacy monitoring and record results in logbook