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Properties of the analysis kit
Also available with Zivak® Multitasker Full Automated Sample Preparation AND Injection System
20 minutes analysis time
Sensitive separation with Zivak® Organic AcidsHPLC column
Quantitative analysis of 55 major organic acids
Between 1500-2000 analysis with one column
No derivatisation, evaporation or SPE process
Suitable for anyLC-MS/MS System
Main methods and procedures that have been selectedare based on EN ISO 13485 and 98/79/EC.
Minimal samplepreparation with rapid,
sensitive and quantitativedetection of 55 organic acids
in 20 minutes
Organic AcidsLC-MS/MS Analysis Kitin Human Urine Samples
METABOLIC DISEASES
[email protected]+90 212 210 62 15 +90 212 210 62 14
ZIVAK TECHNOLOGIES Eski Besiktas Cad. No 44, 34400 ISTANBUL - TURKEY
Distribuido en España por:INGENIERIA ANALITICA S.L. Tel: (+34) 902.45.6677Fax: (+34) 902.46.6677www.ingenieria-analitica.cominf@ingenieria-analitica.com
2-3-pyridine carboxylic2-me-hippuric2-phe-propionic3,4-dihydroxy-hidrocinnamic3-indoleacetic3-me-hippuric acid3-OH-3-me glutaric4-OH-benzoic4-me hippuric4-OH-phenylacetic5-OH-indole-3-aceticAdipicAKBMAKICAKIVAlpha-OH-butyric acidAlpha-KetoglutaricArabitolBenzoicBeta-OH-butyricBeta-OH-isovalericGlucaricCis-aconiticCitramalicCitricEthylmalonicFormiminoglutamicFumaricHippuricHomovanillicIsocitricKynurenicLacticMalicMandelicMethylmalonicOroticPhenylaceticPhenylglyoxylicPicolinicPyroglutamicPyruvic
AnalyteNo
SubericSuccinicTartaricTricarballylVanilmandelicXanthurenicGlycolic AcidGlyceric AcidSuccinyl AcetonPropyonil GlycineIso-Valeryl GlycineGlutaric AcidSebacic Acid
The test results have been validated with an AB Sciex API 4000 LC-MS/MS instrument in Nov. 2014.
LOD(mg/L)
0.0200.0020.0020.0020.0170.0030.0330.0030.0020.0180.0130.0130.0070.0030.0020.0170.0130.0670.0070.0200.0330.0170.1670.0131.6670.0170.0020.0070.0390.0170.0390.0070.0180.0050.0010.0070.0020.0030.0020.0020.0670.0330.0170.0180.0180.0070.0130.0020.0120.0550.0480.0100.0020.0720.022
0.9980.9950.9970.9960.9950.9980.9960.9850.9940.9970.9960.9940.9840.9970.9960.9940.9970.9960.9940.9980.9840.9930.9940.9980.9930.9940.9970.9960.9910.9950.9970.9930.9940.9960.9910.9950.9970.9930.9940.9960.9940.9840.9910.9930.9850.9970.9920.9960.9940.9960.9970.9950.9840.9970.996
Linearity(R2)
Accuracy(%)
95.794.396.592.393.594.692.893.696.591.696.498.297.696.298.494.195.496.594.795.598.493.498.3100.296.795.297.593.294.495.593.794.597.592.597.499.293.892.494.690.591.692.790.991.794.689.894.596.294.295.896.794.894.697.595.1
4.343.214.782.183.174.185.254.543.464.374.383.263.526.743.494.385.273.465.614.233.774.393.865.843.433.253.614.283.274.513.587.533.275.744.504.273.766.254.234.654.273.244.355.463.294.574.174.383.944.216.545.674.354.544.43
PrecisionIntra-Assay
(%CV)
5.325.245.366.135.195.267.165.205.365.096.325.465.427.344.475.235.305.366.515.305.475.195.467.815.375.295.415.185.255.316.569.376.446.356.505.615.787.165.775.885.095.155.056.565.256.515.255.354.205.157.166.505.475.306.15
PrecisionInter-Assay
(%CV)
Validation ResultsLOQ
(mg/L)
0.0660.0060.0050.0060.0550.0110.1100.0110.0060.0580.0440.0440.0220.0110.0060.0550.0410.2200.0220.0660.1100.0550.5500.0445.5000.0550.0060.0220.1290.0550.1290.0220.0580.0170.0040.0220.0060.0110.0060.0060.2200.1100.0550.0580.0580.0220.0440.0060.0360.1550.1520.0300.0060.2120.066
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[email protected]+90 212 210 62 15 +90 212 210 62 14
ZIVAK TECHNOLOGIES Eski Besiktas Cad. No 44, 34400 ISTANBUL - TURKEY
