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New Bioprocess Solutions From Molecule to Market Overcome Your Cell Culture, Purification, and Process Analytics Bottlenecks
Biologics manufacturing processes pose unique challenges.
From improving cell line titers, optimizing media formulations,
and scaling cell culture production to performing same-day critical
impurity analyses and reducing purification run times, we understand
the needs you face every day. The bioprocess manufacturing
solutions offered by Invitrogen and Applied Biosystems are driven by
one single goal—to move you more quickly from molecule to market.
Powerful Upstream, Downstream, and Process Analytics Combinations
Bioreactors HarvestMedia
ColumnChromatography
Bulk DrugSubstance
Harvest/Cell
Removal
FinishedBatch ofActive
Substance
Mycoplasma Detection Residual DNA Quantitation Bacterial and Fungal Identification
SEQ Identification and Detection Systems
VirusClearance
Concentration
Cell Culture Purification Formulation
TransfectionCloneSelection
Expression VectorDevelopment
Cell Banks
Cell Line Development
PD-Direct® Services
GIBCO® Cell Culture Media POROS® Chromatography Solutions From Molecule to Market
Powerful Upstream, Downstream, and Process Analytics Combinations
A complete solution for stable cell line development
OptiCHO™ Protein Express Kit
WHAT IS IT? • Complete set of products and serum-free workflow protocols to go from DNA to a stable protein-expressing CHO cell line
WHY IS IT IMPORTANT? • Save up to 6 months with optimized system and cGMP-documented DG44 parental cell line
• Reduced licensing burden compared to alternatives, no royalties
Contact your Invitrogen Sales Representative
www.invitrogen.com/opticho
Add water.Reduce steps.Boost productivity.
GIBCO® Cell Culture Custom Media and AGT™ Media
WHAT IS IT? • We can produce custom media in liquid batches from 10 L to 10,000 L, dry powder media (DPM) batches
from 1 kg to 8,000 kg, and proprietary Advanced Granulation Technology™ (AGT™) batches from 50 kg to 6,000 kg
WHY IS IT IMPORTANT? • Dual redundant manufacturing sites and robust supply chain strategy help customers
reduce risk as they scale up
• Customers who use AGT™ format love the convenience of a complete product
when compared to dry powder media (DPM)
Contact your Invitrogen Sales Representative
www.invitrogen.com/custommedia
What happens when you pair the finest base medium with the perfect feed supplement?
GIBCO® Catalog Media and Feeding Supplements
WHAT IS IT? • CD OptiCHO™ Medium and the CHO CD EfficientFeed™ Kit provide a base medium and feeding supplements that can
shorten the path to optimizing process development conditions at bench scale and assist with seamless scale-up
WHY IS IT IMPORTANT? • Chemically defined consistency without sacrificing performance
• 2- to 5-fold higher fed-batch productivity
Contact your Invitrogen Sales Representative
www.invitrogen.com/opticho
Experience you trust—at high speed
GIBCO® MediaExpress™ Custom Media
WHAT IS IT? • GIBCO® MediaExpress™ (GME) Custom Media offers GIBCO® quality custom media at high speed
• GME offers liquid batches from 1 L to 200 L, DPM batches from 1 kg to 5 kg, and AGT™ batches from 2 kg to 10 kg
WHY IS IT IMPORTANT? • Small volumes and quick turnaround give flexibility for process development customers to test multiple
formulations before scaling up to cGMP
• Fixed pricing offers ease in ordering
Contact your Invitrogen Sales Representative
www.invitrogen.com/mediaexpress
less
More confidence and clarity for every phase of your drug development
PD-Direct® Bioprocess Services
WHAT IS IT? • PD-Direct® Bioprocess Services offers outsourcing solutions for cell line development, cell culture media
optimization, and process optimization
WHY IS IT IMPORTANT? • The service offers customers the opportunity to access leading process development technologies
(i.e., SimCell™, ClonePix™, and Revolution® technologies) to improve process performance and accelerate time to market
• Customers receive expert technical consultation on process development problems, applications support
of Invitrogen products, and comprehensive service-based solutions
For more information, contact [email protected].
Acholeplasma granularum • Acholeplasma laidlawii • Acholeplasma pleciae • Mycoplasma alkalescens • Mycop
Mycoplasma alvi • Mycoplasma arginini • Mycoplasma auris • Mycoplasma buccale • Mycoplasma californicum •
Mycoplasma canadense • Mycoplasma capricolum • Mycoplasma caviae • Mycoplasma collis • Mycoplasma fer-
mentans • Mycoplasma equirhinis • Mycoplasma fermentans • Mycoplasma gallicum • Mycoplasma gallisepticum •
Mycoplasma gateae • Mycoplasma genitalium • Mycoplasma gypis • Mycoplasma hominis • Mycoplasma
Mycoplasma hominis • Mycoplasma indiense • Mycoplasma lagogenitalium • Mycoplasma hyorhinis • Spiroplasm
Spiroplasma citri • Mycoplasma molare • Mycoplasma mycoides • Mycoplasma neurolyticum • Mycoplasma orale •
Mycoplasma pirum • Mycoplasma pneumoniae • Mycoplasma salivarium • Mycoplasma spumans • Mycoplasma pi-
rum • Acholeplasma granularum • Acholeplasma laidlawii • Acholeplasma pleciae • Mycoplasma alkalescens • Mycop
Mycoplasma alvi • Mycoplasma arginini • Mycoplasma auris • Mycoplasma buccale • Mycoplasma californicum •
Mycoplasma canadense • Mycoplasma capricolum • Mycoplasma caviae • Mycoplasma collis • Mycoplasma fer-
mentans • Mycoplasma equirhinis • Mycoplasma fermentans • Mycoplasma gallicum • Mycoplasma gallisepticum •
Mycoplasma gateae • Mycoplasma genitalium • Mycoplasma gypis • Mycoplasma hominis • Mycoplasma
Mycoplasma hominis • Mycoplasma indiense • Mycoplasma lagogenitalium • Mycoplasma hyorhinis • Spiroplasm
Spiroplasma citri • Mycoplasma molare • Mycoplasma mycoides • Mycoplasma neurolyticum • Mycoplasma orale •
Mycoplasma pirum • Mycoplasma pneumoniae • Mycoplasma salivarium • Mycoplasma spumans • Mycoplasma pi-Mycoplasma detection in hours, not days. It’s about time.
Acholeplasma laidlawii
Mycoplasma buccale
Mycoplasma fermentans
Mycoplasma gateae
Mycoplasma hyorhin
Mycoplasma molare
Mycoplasma salivarium
Mycoplasma auris
Mycoplasma canadense
Mycoplasma gallicum
Mycoplasma indiense
MycoSEQ™ Mycoplasma Detection System
WHAT IS IT?
• An integrated sample preparation and real-time, quantitative PCR assay for the detection of mycoplasmas
in cell culture samples
• Complete system includes sample preparation, assay, and instrument
WHY IS IT IMPORTANT?
• Mammalian cell culture manufacturers are required to test for potential mycoplasma contamination;
the current mycoplasma test is a 28-day culture test
• The MycoSEQ™ Mycoplasma Detection System delivers actionable results in 5 hours, allowing for
valuable in-process testing during cell culture development
Contact your Applied Biosystems Sales Representative
www.microseq.com/mycoplasma
Go molecular. The answer is in the DNA.
MicroSEQ® Microbial Identification System
WHAT IS IT?
• Fastest and most accurate commercially available identification system for bacteria and fungi, based
on DNA sequencing of the 16S rRNA gene for bacteria or the D2 region of the large subunit for fungi
• Complete system includes sample preparation, assays, instrument, and software
WHY IS IT IMPORTANT?
• Harmful bacteria and fungi can contaminate raw materials, production facilities, and branded products
• Regulatory expectations include an active environmental monitoring program as part of cGMP
to identify bacteria and fungi at the species level
Contact your Applied Biosystems Sales Representative
www.microseq.com
Residual host cell DNA quantitation. Precision counts.
resDNASEQ™ CHO Residual DNA Quantitation System
WHAT IS IT?
• First integrated qPCR system for quantitation of CHO residual DNA with optional automated
sample preparation
• Complete system includes sample preparation, TaqMan® assay, genomic CHO standard, and instrument
WHY IS IT IMPORTANT?
• Regulatory expectation is that DNA content in the final product should be as low as possible, as
determined by a highly sensitive method
• The resDNASEQ™ CHO Residual DNA System delivers high DNA recovery and same-day results easily,
in <5 hours
Contact your Applied Biosystems Sales Representative
www.microseq.com
Transform your downstream performance.
POROS® Perfusion Chromatography Media
WHAT IS IT? • Best-performing chromatographic media for process-scale bioseparations
• Patented “throughpores” enable high-resolution separations with 2–3x the throughput of conventional
fast-flow gels
WHY IS IT IMPORTANT? • Helps overcome purification challenges for monoclonal antibodies and other biotherapeutic molecules
• Rigid and robust; easy to handle and pack
• Used in multiple FDA-approved therapeutics
Contact your Applied Biosystems Sales Representative
www.info.appliedbiosystems.com/poros
Contact Information
For research use only. Not for human or animal therapeutic or diagnostic use.
© 2009 Life Technologies Corporation. All rights reserved. Trademarks of Life Technologies Corporation and its affiliated companies: AB (Design)®, Advanced Granulation Technology™, AGT™, Applied Biosystems®, EfficientFeed™, GIBCO®, Invitrogen™, MediaExpress™, MicroSEQ®, OPtiCHO™, PD-Direct®, POROS®, resDNASEQ™, Revolution®. TaqMan is a registered trademark of Roche Molecular Systems, Inc. All other trademarks are the sole property of their respective owners.
Printed in the USA, 07/2009 Publication 128BR09-01 O-085463
