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Darlington_Supplemental Figure 1. Atm -/- Wip1 +/+. Atm -/- Wip1 -/-. Atm +/+ Wip1 +/+. Atm +/+ Wip1 -/-. p-p53 (p18S). p53. γ H2AX (p140S). H2AX. tubulin. 5 Gy IR. -. -. -. -. -. -. -. +. +. +. -. +. +. +. +. +. Darlington_Supplemental Figure 2. Atm -/- Wip1 -/-. - PowerPoint PPT Presentation
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p-p53 (p18S)
p53
γH2AX (p140S)
H2AX
tubulin
5 Gy IR - - - - - - - -+ + + + + + + +
Atm+/+Wip1+/+ Atm+/+Wip1-/- Atm-/-Wip1+/+ Atm-/-Wip1-/-
Darlington_Supplemental Figure 1
5 Gy IR
Atm+/+
Wip1+/+Atm+/+
Wip1-/-Atm-/-
Wip1+/+Atm-/-
Wip1-/-
- - + + - - - - - -+ + + + + +p-Brca1
(p1423S)
Brca1
tubulin
Darlington_Supplemental Figure 2
0
20
40
60
80
100
no IR +IR no IR +IR no IR +IR no IR +IR
WT Wip-/- Atm-/- DKO
5 Gy IR
Atm+/+
Wip1+/+Atm+/+
Wip1-/-Atm-/-
Wip1+/+Atm-/-
Wip1-/-
- - ++ + + + + + +- - - - - -cleaved PARP1
cleaved caspase-3
tubulin
Cleaved PARP1 protein levels (normalized to tubulin)
Arb
itra
ry u
nit
Cleaved Caspase-3 protein levels (normalized to tubulin)
0
20
40
60
80
100
no IR +IR no IR +IR no IR +IR no IR +IR
WT Wip-/- Atm-/- DKO
Arb
itra
ry u
nit
A
B C
Atm+/+
Wip1+/+Atm+/+
Wip1+/+Atm+/+
Wip1-/-Atm+/+
Wip1-/-Atm-/-
Wip1+/+Atm-/-
Wip1+/+Atm-/-
Wip1-/-Atm-/-
Wip1-/-
5 Gy IR 5 Gy IR- - - - - - - -+ + + + + + + +
100 100
80 80
60 60
40 40
20 20
0 0
Darlington_Supplemental Figure 3