PCB Soil Field Sampling Plan - Yankee Rowe (PCB) Soil Field Sampling Plan (FSP) for the Yankee Nuclear Power Station ... of PCB-containing paint chips. Approximately 3,500 cubic yards

PCB Soil Field Sampling Plan

Yankee Nuclear Power Station 49 Yankee Road Rowe Massachusetts

February 2005

1.0 INTRODUCTION 1

1.1 BACKGROUND 1

1.2 PURPOSE & SCOPE 1

1.3 SUMMARY OF EXISTING DATA 2

2.0 SOIL SAMPLING PROGRAM 3

2.1 STANDARD OPERATING PROCEDURES 3

2.2 SAMPLE LOCATIONS AND DESIGNATIONS 3

2.3 SAMPLING PROCEDURES AND ANALYTICAL PROGRAM 5 2.3.1 Geoprobe Sampling 5 2.3.2 Auger Sampling 6

2.4 ANALYTICAL PROGRAM 6 2.4.1 On-Site PCB Screening 6 2.4.2 PCB Laboratory Analyses 7 2.4.3 Radiological Parameters 8

2.5 SAMPLE SECURITY AND CUSTODY 8

2.6 MANAGEMENT OF INVESTIGATION DERIVED WASTES 8

2.7 SCHEDULE 8

3.0 QUALITY ASSURANCE AND QUALITY CONTROL 9

3.1 QUALITY ASSURANCE PROJECT PLAN 9

3.2 CLEANING AND DECONTAMINATION OF EQUIPMENT 9

3.3 QUALITY ASSURANCE / QUALITY CONTROL SAMPLES 10

4.0 PROJECT DOCUMENTATION 12

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TABLES

Table 1 PCB Soil Results Table 2 Soil PCB Analytical Program

FIGURES

Figure 1 Site Locus Figure 2 PCB Soil Sampling Summary Figure 3 Proposed Sampling Locations

APPENDICES

Appendix A Gradient Memorandum on Sampling Appendix B Rapid Assay Instructions Appendix C Results Comparison between NEL Off Site Lab Analysis and

Rapid Assay Immunoassay PCB Soil Sample Analysis

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PCB Soil Field Sampling Plan

Yankee Nuclear Power Station

49 Yankee Road

Rowe Massachusetts

February 2005

____________________________________

Kenneth Dow

Yankee Atomic Environmental Manager

_____________________________________

John. W. McTigue, P.G., LSP

Environmental Resources Management

ERM YANKEE 2/15/2005

1.0 INTRODUCTION

1.1 BACKGROUND

On behalf of Yankee Atomic Electric Company (YAEC), Environmental Resources Management (ERM) has prepared this Polychlorinated Biphenyl (PCB) Soil Field Sampling Plan (FSP) for the Yankee Nuclear Power Station (YNPS) located at 49 Yankee Road in Rowe, Massachusetts (Figure 1). This FSP has been prepared as a supplement to the Quality Assurance Project Plan (QAPP), Site Closure, Revision 2, Yankee Nuclear Power Station, Rowe, Massachusetts, Gradient, dated 6 August 2004.

1.2 PURPOSE & SCOPE

The purpose of the PCB Soil FSP is to delineate the lateral and vertical extent of PCB impacts to soil greater than 1 milligram per kilogram (mg/kg) in the vicinity of the Industrial Area at the YNPS. New data generated as a result of this sampling program will be used to define the remedial strategy for PCB-impacted soil and to develop a plan to integrate the remedial activities into the on-going plant decommissioning activities. The sampling program is intended to support closure of the site under the Massachusetts Contingency Plan (MCP) and Toxic Substances Control Act (TSCA).

The PCB Soil FSP is intended to:

• Establish the procedures and rationale for soil sampling activities in support of site closure.

• Establish requirements, protocols and documentation for protection of worker health and safety.

• Ensure that soil sampling is consistent with applicable procedures.

• Ensure that the Data Quality Objectives (DQOs) for site closure are met.

Areas not addressed in the PCB Soil FSP include:

• PCB impacts to the SCFA, which is the focus of separate investigation program.

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• Low-level PCB impacts in areas distant from the Industrial Area where levels are consistently below 1 mg/kg and there is no known source of impact.

• Radiological analyses, which are the focus of a separate investigation program.

• Post-remediation closure sampling to satisfy TSCA, which will be performed during a later phase of investigation.

• Sampling of PCB work areas, including crane pads, which will be performed during a separate sampling event.

The data generated under this FSP will be validated and input into the site database and used in conjunction with historic data to determine the need for, and scope of, future soil sampling events and remedial response actions.

1.3 SUMMARY OF EXISTING DATA

Soil samples have been collected at more than 180 locations for PCB analysis. The majority of samples have been in the upper 12 inches of soil, with sampling at select locations up to 15 feet below ground surface (bgs).

Soil excavation activities were conducted in Fall 2004 in the central portion of the site, below the former Vapor Container, which is the primary source of PCB-containing paint chips. Approximately 3,500 cubic yards of soil have been excavated and are currently stored on-site.

A summary of the existing PCB soil data is provided in Table 1 and Figure 2. The sampling data has been collected as part of the MCP and site closure investigation activities. PCBs were less than 1 mg/kg in 82 percent of the samples. The maximum concentration of PCBs detected at the site is 240 mg/kg (YRDTS-22).


2.0 SOIL SAMPLING PROGRAM

2.1 STANDARD OPERATING PROCEDURES

YNPS Standard Operating Procedures (SOPs) applicable to the soil investigation include:

• YNPS Health and Safety Plan.

• DP-8120 Collection of Site Characterization and Site Release Samples.

• DP-8123 Sample Security and Chain of Custody.

• AP-0626 Job Hazard Assessments.

• AP-8122 Subsurface Soil Sampling and Monitoring Well Installation.

All YNPS SOPs applicable to this FSP are maintained in the YNPS Environmental Oversight Trailer.

2.2 SAMPLE LOCATIONS AND DESIGNATIONS

Due to the range of PCB concentrations detected and the size of YNPS, the site was subdivided into five study areasi which are intended to exhibit less variation in the range of PCBs detected. Within each study area a sampling program was developed based on the following criteria:

• Coverage and variation of existing data.

• Presence of concrete slabs that were present throughout life of plant.

• Feasibility of collecting additional samples (based on topography and winter conditions).

• Observations of paint chips in soil (i.e., adjacent to select building foundations).

Gradient performed an analysis of the existing PCB data in an effort to develop the basis for the number of samples (see Appendix A). Based on

i The concept of study areas are being used for the purposes of developing the PCB

Soil FSP and are not intended for risk assessment purposes.


this analysis, 20 samples per study area are expected to be sufficient to provide obtain a 95 percent upper confidence limit of a mean of 1 mg/kg.

Sample locations were selected based on the following basis:

• Grid Sampling - Sample locations were selected to fill existing data gaps. Samples will be collected both on the grid that was created for the site closure sampling program and in targeted areas. The grid sampling will be conducted on a 50-foot grid, where appropriate, to supplement the original 100-foot grid used for the site closure sampling program.

• Targeted Sampling – Sampling will be targeted to supplement the grid based on the existing data and adjacent to structures where paint chips were observed on the ground surface.

• Replicate Sampling – Four sample locations are intended to replicate previous sampling data that appears anomalous: SB-026, SB-092, YRDTS-020, and YRDTS-043.

A summary of the proposed sampling activities is provided below: Study area

Maximum Concentration

(mg/kg)

# of Existing Samples

# of New Sample

Locations

Comments

1 240 34 38 Samples along grid, below excavation area, and in targeted area to east of former Vapor Container

2 12 41 34 Samples along hillside along grid. Three samples below roadway to determine if impacts are present below roadway.

3 4 33 20 Samples along grid primarily in eastern portion of area, with some confirmatory sample in western portion.

4 3 22 12 Sampling north and east of service building to evaluate potential impacts associated with decommissioning activities.

5 2 18 11 Sampling south of Service Building and along grid to east of central portion of site.


Sample locations within the central portion of the site (Study areas 1, 3, and 4, and in portions of 2 and 5) will be collected using a Geoprobe drill rig. Soil samples will be collected from ground surface to 4 feet bgs at most locations. At five of the sampling locations, the boring will extend to 8 feet bgs to verify the vertical extent of impact. Soil samples will be collected from the following intervalsii: 0 to 3 inches bgs, 1 to 2 feet bgs, 2 to 3 feet bgs, 3 to 4 feet bgs, 4 to 6 feet bgs, and 6 to 8 feet bgs, as appropriate. A sample may also be collected between 3 and 12 inches below grade based on field observations.

Samples in the remaining areas will be collected to from 0 to 3 inches bgs, except where noted in Table 2. Those samples will be collected using a hand auger or Geoprobe.

The soil samples will be identified using unique sample identification. The sample designations will use the naming convention as detailed in the YNPS QAPP and in Table 2. Sample locations will be recorded using a Global Positioning System (GPS) unit or will be flagged and surveyed. Where feasible, the sample locations will also be marked with a flag, stake, or paint.

The soil sample locations are shown in Figure 3. The locations detailed on the figures are approximate and may be adjusted based on field observations. Access to some locations may be restricted due to the on-going decommissioning activities.

2.3 SAMPLING PROCEDURES AND ANALYTICAL PROGRAM

2.3.1 Geoprobe Sampling

Geoprobe soil samples will be collected under Yankee oversight in compliance with YNPS Procedure DP-8120 Collection of Site Characterization and Site Release Samples. Direct push techniques will be used to push the two-inch diameter core sampler into the subsurface. The hollow two-inch diameter macro-core is pushed by a secondary set of

ii The sample depths are to be from existing grade, with the exception of areas where

fill has recently been placed or soils have been excavated as part of decommissioning. At locations where fill has been placed, samples will begin at the interface of the new fill and the original grade. At locations where excavations have occurred, the samples will begin at the current grade and a note will be made regarding the estimated depth of the sample location below original grade.


solid one-inch diameter rods. The rods/macro-core receive the driving force from the percussion hammer of the Geoprobe. A disposable polyethylene sleeve within the macro-core retains the sample. Once the desired depth is reached, the rods/macro-core are retrieved. The sleeve is removed from the macro-core and the portion of the retrieved soil materials to be sampled is removed from the polyethylene sleeve and the remaining soil materials will be used to backfill the boring. The portion of the core to be submitted for laboratory analysis will be visually inspected and the soil stratigraphy will be classified. Visual observations will be logged on soil sampling forms.

Dedicated sampling equipment will be used where feasible. Reused sampling equipment will be decontaminated prior to, and following, sample collection in accordance with the YNPS QAPP.

2.3.2 Auger Sampling

Soil samples will be collected manually using a hand auger to a depth of up to 2 feet bgs at select locations where it is not feasible to use the Geoprobe. Samples will be collected in compliance with YNPS Procedure DP-8120 Collection of Site Characterization and Site Release Samples. Samples will be collected from 0 to 3 inches bgs at all locations, and from 1 to 2 feet bgs at select locations (as listed in Table 2). The portion of the core to be submitted for laboratory analysis will be visually inspected and the soil stratigraphy classified. Visual observations, field observations, and soil stratigraphy description results will be recorded on sample collection logs.

2.4 ANALYTICAL PROGRAM

2.4.1 On-Site PCB Screening

All soil samples from Study Area 1 and the two upper soil samples (0 to 3 inches and 1 to 2 feet) from all other study areas will be screened on-site for PCBs using the Rapid Assay Test System, manufactured by Strategic Diagnostics Inc., a magnetic particle immunoassay technique. The need for conducting screening on deeper samples will be evaluated based on the results of the screening of the upper two intervals and the existing data.

A copy of the user manual for the Rapid Assay Test is provided in Appendix B. YAEC has conducted a site-specific comparison between the screening technique and laboratory data and found them to be suitable for


screening purposes (see Appendix C). The screening method should not be used if paint chips are visible in the soil sample.

2.4.2 PCB Laboratory Analyses

Laboratory analysis will be used to confirm that PCB levels are below 1 mg/kg in areas at the site that are not being disturbed by the on-going decommissioning activities. The following soil samples will be submitted for laboratory analysis:

• All samples with visible paint chips.

• Soil samples from Study areas 2, 4, and 5 that have reported PCB concentrations less than 1 mg/kg based on the screening method.

• Other select samples, as deemed appropriate based on the screening results.

Select samples not submitted for laboratory analysis will be preserved for potential future analysis by freezing the samples at the site (consistent with Method 8082MCP-Rev 4). Samples will be frozen within 24 hours of collection at less than -10˚ Celsius. Sampling container should only be filled to 2/3 of capacity to avoid breakage caused by expansion during freezing. Preparation or extraction should be commenced within 24 hours of thawing.

Each soil samples will be placed into a 4-ounce sample jar and chilled to 4˚ Celsius, or less. Soil samples will be submitted for PCB analysis by GC, SW-846 Method 8082, with extraction by Method 3540-C (soxhlet extraction). Northeast Laboratory Services, located in Waterville, Maine, will conduct the analyses. Contact information for the laboratory is as follows: Northeast Laboratories Project Manager: Kelly Perkins PO Box 788 Waterville, Maine 04903 Phone: 800-244-8378 ext. 307 YAEC is in the process of evaluating the use of additional laboratories. If another laboratory is used, the QAPP will be updated, as appropriate.


2.4.3 Radiological Parameters

Samples at select locations may also be submitted for radiological analysis. The radiological sample program is in the process of being prepared.

2.5 SAMPLE SECURITY AND CUSTODY

Soil samples will be submitted to the laboratory or YNPS Personnel under proper chain-of-custody procedures. Samples will be preserved on ice or in a refrigerator and sample handling will be documented using chain-of-custody protocols in accordance with DP-8123, Sample Security and Chain of Custody.

2.6 MANAGEMENT OF INVESTIGATION DERIVED WASTES

Soil sampling waste, such as sampling equipment rinse water, will be containerized on-site. The wastes will be screened for radiological constituents. Following screening YNPS personnel will dispose of the wastes in accordance with the applicable YNPS procedures.

2.7 SCHEDULE

The soil sampling program described is scheduled to begin in February 2005. The completion schedule for the soil sampling activities is dependent on field conditions and accessibility to sample locations. Field Sampling Reports should be prepared immediately following sampling to summarize field observations to enable rapid analysis of analytical results upon receipt and expedite decisions regarding further laboratory analyses and the need for additional investigation, if necessary.


3.0 QUALITY ASSURANCE AND QUALITY CONTROL

3.1 QUALITY ASSURANCE PROJECT PLAN

A QAPP has been prepared to provide a standard method of assuring that data collected during site characterization activities is of sufficient quality to support future decisions regarding decommissioning activities and or remedial actions at the site. The primary purpose of the QAPP is to describe the means by which data collected in the field will be validated against predetermined standards, ensuring that data meets minimum quality standards prior to being used for decision-making purposes. The flow of data is important to data quality, as it ensures that appropriate project personnel have adequate opportunities to review data with importance to future site decisions. As such, the QAPP specifies the methods and means for ensuring the data generated during site characterization activities is of a quality necessary to serve its intended purpose.

The following provides a list of the sections of the QAPP that are most relevant to the field sampling activities:

QAPP Section Topic

9.1 Field Investigation and Documentation Procedures

9.2 Preparation of Sample Containers

9.3 Decontamination

9.4 Field Equipment Usage and Maintenance

10.1 Sample Tracking System

10.2 Sample Custody

13.1 Field Quality Control

3.2 CLEANING AND DECONTAMINATION OF EQUIPMENT

To the degree possible, dedicated and/or disposable sampling equipment will be used for sampling. Non-dedicated sampling equipment used to


collect samples will be cleaned and decontaminated prior to its initial use, between each sampling location and after the final use. The following general procedures will be adhered to concerning decontamination efforts:

1. Non-dedicated sampling equipment will be rinsed with DI water and Alconox to remove visible contamination. A methanol rinse and then a DI water rinse will be used to remove contamination. See the QAPP for specific decontamination procedures.

2. If visual signs such as discoloration indicate that decontamination was insufficient, the equipment will again be decontaminated. If the situation persists, the equipment will be taken out of service until the situation can be corrected.

3. Verification of the non-dedicated sampling equipment cleaning procedures will be documented by the collection of field equipment rinsate blanks, at a frequency in accordance with the QAPP.

4. Properly decontaminated equipment will be stored in aluminum foil or plastic bags during storage and transport.

Decontamination protocols will be strictly adhered to in order to minimize the potential for cross-contamination between sampling locations and contamination of off-site areas. Liquids generated during the decontamination process will be collected, containerized and appropriately labeled for disposal. Waste liquids will be stored on site until determination of potential hazard class and final disposition.

Only pre-cleaned laboratory-certified sample containers will be used. The laboratories will also provide sample coolers, ice packs, trip blanks, and temperature blanks.

More specific decontamination procedures are outlined in the QAPP and SOPs.

3.3 QUALITY ASSURANCE / QUALITY CONTROL SAMPLES

The following Quality Assurance / Quality Control samples will be collected during the soil sampling:

ERM 10 YANKEE 2/15/2005

• Temperature blanks – One temperature blank per cooler. The temperature of the temperature blank will be measured upon receipt of the cooler at the laboratory.

• Equipment rinsate blank – Manual sampling will be conducted using a hand auger. A rinsate sample will be collected from the hand auger at a rate of one sample per 20 sampling locations. The rinsate blanks will be analyzed for the same parameters as the samples that were collected using the equipment.

• Field duplicates – Field duplicates will be collected at the rate of one duplicate per 20 samples. Samples locations where duplicates will be collected will be determined in the field. Field duplicates will be submitted for the same analyses as the actual sample. To the extent practicable, field duplicate samples are predetermined and target the highest analytical results from previous soil investigations. A field duplicate will be collected for each analysis detailed in this FSP.

• Matrix spikes – Matrix spikes will be collected at the rate of one matrix spike per 20 samples. Samples locations where matrix spikes will be collected will be determined in the field. Matrix spike/matrix spike duplicates (MS/MSDs) will be collected for organic parameters. Matrix spike/matrix duplicates (MS/MD) will be collected for inorganic and wet chemistry analyses.

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4.0 PROJECT DOCUMENTATION

Data management tasks pertinent to project documentation and records, laboratory deliverables, data reporting formats, data handling and management, and data review assessment are presented in the QAPP.

The following field sample collection records will be completed at the time of sample collection and documented in a Field Sampling Report:

• Sample location identification

• Soil stratigraphy

• Observations of any notable conditions encountered in materials retrieved (e.g., presence of visible paint chips)

• Modifications to the FSP

• GPS coordinates and a map indicating approximate locations

COC forms will be completed post sampling by the field sampling team. Within 24 hours of COC completion, copies of the COCs will be faxed to the following people.

• Kim Reed – Gradient Corporation, (617) 395-5001

• Andy Coenen – ERM, (631) 756-8901

• Jeremy Picard – ERM, (617) 267-6447

The project documentation will be provided to the Yankee Environmental Oversight Supervisor (Deanne Stefanovich) at the completion of each sampling event. In addition, any deviations from the FSP will be documented in a memorandum to the Yankee Environmental Operations Manager (Marynette Herndon).

The Yankee Environmental Oversight Supervisor will forward copies of the project documentation, the Field Sampling Report to Jeremy Picard (ERM).

Table 1PCB Soil ResultsYankee Nuclear Power StationRowe, MA

Station Sample ID Date Depth Aroclor-1254 Aroclor-1260 Total PCBs(mg/Kg) (mg/Kg) (mg/Kg)

YRDTS-001 YRDTS-001 10/18/2000 0-2" 0.2 U 0.2 U NDYRDTS-002 YRDTS-002 10/18/2000 0-2" 0.2 0.2 U 0.2YRDTS-003 YRDTS-003 10/18/2000 0-2" 0.2 U 0.2 U NDYRDTS-004 YRDTS-004 10/18/2000 0-2" 0.2 U 0.2 U ND

YRDTS-005 10/24/2000 0-2" 4.7 0.2 U 4.7YRDTS-005-4-6 11/20/2000 4-6" 42 4 U 42

YRDTS-005-4-6RE 11/20/2000 4-6" 40 4 U 40YRDTS-005A 0-2 in 11/11/2002 0-2" 47 3.7 U 47YRDTS-005A-Dup-1 11/11/2002 0-2" 39 1.8 U 39YRDTS-005B 2-4 in 11/11/2002 2-4" 42 1.8 U 42

YRDTS-005C 6-12 in 11/11/2002 6-12" 1.4 0.36 U 1.4YRDTS-005D 12-18 in 11/11/2002 12-18" 0.79 0.036 U 0.79

YRDTS-006-4-6 11/20/2000 4-6" 1.7 0.2 U 1.7YRDTS-006A 10/24/2000 0-2" 11 0.2 U 11YRDTS-006B 10/24/2000 4-6" 0.4 0.2 U 0.4

YRDTS-006A 0-2 in 11/11/2002 0-2" 1.4 0.053 U 1.4YRDTS-006A-DUP-2 2-4 in 11/11/2002 2-4" 1.1 0.049 U 1.1

YRDTS-006B 2-4 in 11/11/2002 2-4" 0.091 0.043 U 0.091YRDTS-006C 6-12 in 11/11/2002 6-12" 0.04 U 0.04 U ND

YRDTS-006D 12-18 in 11/11/2002 12-18" 0.04 U 0.04 U NDYRDTS-007 YRDTS-007 10/24/2000 0-2" 1.1 0.2 U 1.1YRDTS-008 YRDTS-008 10/24/2000 0-2" 0.2 U 0.2 U NDYRDTS-009 YRDTS-009 10/24/2000 0-2" 0.3 0.2 U 0.3YRDTS-010 YRDTS-010 10/24/2000 0-2" 0.2 U 0.2 U NDYRDTS-011 YRDTS-011 10/24/2000 0-2" 0.2 U 0.2 U NDYRDTS-12 YRDTS-12 10/24/2000 0-2" 0.2 U 0.2 U NDYRDTS-13 YRDTS-13 10/24/2000 0-2" 0.7 0.2 U 0.7

YRDTS-14 10/24/2000 0-2" 2.6 0.2 U 2.6YRDTS-014-4-6 11/20/2000 4-6" 0.22 0.2 U 0.22

YRDTS-15A 10/24/2000 0-2" 0.2 U 0.2 U NDYRDTS-15B 10/24/2000 4-6" 0.2 U 0.2 U NDYRDTS-016 11/15/2000 0-2" 1.4 0.2 U 1.4

YRDTS-016-4-6 11/20/2000 4-6" 12 0.2 U 12YRDTS-016A 11/15/2000 0-2" 2.4 0.2 U 2.4

YRDTS-016A 0-2 in 11/11/2002 0-2" 1.5 0.046 U 1.5YRDTS-016B 2-4 in 11/11/2002 2-4" 2.6 0.21 U 2.6


YRDTS-017 11/15/2000 0-2" 2.8 0.2 U 2.8YRDTS-017-4-6 11/20/2000 4-6" 0.85 0.2 U 0.85

YRDTS-018 11/15/2000 0-2" 2.5 0.2 U 2.5YRDTS-018A 0-2 in 11/11/2002 0-2" 1.8 0.18 U 1.8YRDTS-018B 2-4 in 11/11/2002 2-4" 0.6 0.036 U 0.6


YRDTS-019 11/15/2000 0-2" 2.7 0.2 U 2.7YRDTS-019-4-6 11/20/2000 4-6" 0.52 0.2 U 0.52YRDTS-020-0-2 11/20/2000 0-2" 8.6 J 0.2 U 8.6YRDTS-020-4-6 11/20/2000 4-6" 0.33 0.2 U 0.33

YRDTS-021 YRDTS-021-0-2 11/20/2000 0-2" 0.69 J 0.2 U 0.69YRDTS-022-0-2 11/20/2000 0-2" 240 10 U 240

YRDTS-022-0-2RE 11/20/2000 0-2" 240 10 U 240YRDTS-022-4-6 11/20/2000 4-6" 76 4 U 76

YRDTS-022A 0-2 in 11/11/2002 0-2" 120 3.5 U 120YRDTS-022A Dup-3 0-2 11/11/2002 0-2" 27 3.7 U 27

YRDTS-022B 2-4 in 11/11/2002 2-4" 18 1.8 U 18YRDTS-022C 6-12 in 11/11/2002 6-12" 0.32 0.036 U 0.32

YRDTS-022D 12-18 in 11/11/2002 12-18" 2 0.36 U 2

YRDTS-005

YRDTS-006

YRDTS-016

YRDTS-017

YRDTS-15

YRDTS-14

YRDTS-019

YRDTS-020

YRDTS-022

YRDTS-018

Page 1 of 6



YRDTS-023-0-2 11/20/2000 0-2" 1 0.2 U 1YRDTS-023-4-6 11/20/2000 4-6" 3 0.2 U 3

YRDTS-023A 0-2 in 11/11/2002 0-2" 4.3 0.4 U 4.3YRDTS-023B 2-4 in 11/11/2002 2-4" 2.2 0.36 U 2.2


YRDTS-024-0-2 11/20/2000 0-2" 2.9 J 0.2 U 2.9YRDTS-024-4-6 11/20/2000 2-4" 0.85 0.2 U 0.85

YRTDS-025 YRDTS-025-0-2 5/16/2001 0-2" 0.78 U 0.78 U NDYRTDS-026 YRDTS-026-0-2 5/16/2001 0-2" 1.3 0.34 U 1.3YRTDS-027 YRDTS-027-0-2 5/16/2001 0-2" 0.24 0.22 U 0.24YRTDS-028 YRDTS-028-0-2 5/16/2001 0-2" 1.2 0.52 U 1.2YRTDS-029 YRDTS-029-0-2 5/16/2001 0-2" 0.45 0.24 U 0.45YRTDS-030 YRDTS-030-0-2 5/16/2001 0-2" 0.6 U 0.6 U ND

YRDTS-031A 0-2 in 11/11/2002 0-2" 16 0.36 U 16YRDTS-031B 2-4 in 11/11/2002 2-4" 16 0.69 16.69YRDTS-032A 0-2 in 11/11/2002 0-2" 0.17 0.04 U 0.17YRDTS-032B 2-4 in 11/11/2002 2-4" 0.91 0.36 U 0.91YRDTS-033A 0-2 in 11/5/2002 0-2" 0.36 U 0.36 U NDYRDTS-033B 2-4 in 11/5/2002 2-4" 0.4 U 0.4 U NDYRDTS-034A 0-2 in 11/5/2002 0-2" 0.16 0.04 U 0.16YRDTS-034B 2-4 in 11/5/2002 2-4" 0.081 0.04 U 0.081YRDTS-035A 0-2 in 11/5/2002 0-2" 2.2 0.2 U 2.2YRDTS-035B 2-4 in 11/5/2002 2-4" 1.1 0.04 U 1.1YRDTS-036A 0-2 in 11/5/2002 0-2" 0.34 0.043 U 0.34YRDTS-036B 2-4 in 11/5/2002 2-4" 0.16 0.043 U 0.16YRDTS-037A 0-2 in 11/5/2002 0-2" 0.46 0.043 U 0.46YRDTS-037B 2-4 in 11/5/2002 2-4" 0.14 0.043 U 0.14YRDTS-038A 0-2 in 11/4/2002 0-2" 0.04 U 0.04 U NDYRDTS-038B 2-4 in 11/4/2002 2-4" 0.04 U 0.04 U NDYRDTS-039A 0-2 in 11/4/2002 0-2" 0.051 0.04 U 0.051YRDTS-039B 2-4 in 11/4/2002 2-4" 0.036 U 0.036 U NDYRDTS-040A 0-2 in 11/5/2002 0-2" 0.043 U 0.043 U NDYRDTS-040B 2-4 in 11/5/2002 2-4" 0.22 0.043 U 0.22YRDTS-041A 0-2 in 11/5/2002 0-2" 2.1 0.27 U 2.1YRDTS-041B 2-4 in 11/5/2002 2-4" 0.93 0.04 U 0.93YRDTS-042A 0-2 in 11/5/2002 0-2" 0.72 0.043 U 0.72YRDTS-042B 2-4 in 11/5/2002 2-4" 0.51 0.043 U 0.51YRDTS-043A 0-2 in 11/5/2002 0-2" 1.6 0.043 U 1.6YRDTS-043B 2-4 in 11/5/2002 2-4" 0.64 0.049 U 0.64YRDTS-044A 0-2 in 11/11/2002 0-2" 0.75 0.36 U 0.75YRDTS-044B 2-4 in 11/11/2002 2-4" 0.041 0.036 U 0.041YRDTS-045A 0-2 in 11/11/2002 0-2" 0.24 0.04 U 0.24YRDTS-045B 2-4 in 11/11/2002 2-4" 0.15 0.04 U 0.15YRDTS-046A 0-2 in 11/11/2002 0-2" 0.055 0.04 U 0.055YRDTS-046B 2-4 in 11/11/2002 2-4" 0.048 0.04 U 0.048YRDTS-047A 0-2 in 11/11/2002 0-2" 0.033 U 0.25 0.25YRDTS-047B 2-4 in 11/11/2002 2-4" 0.4 0.36 U 0.4YRDTS-048A 0-2 in 11/5/2002 0-2" 0.036 U 0.036 U NDYRDTS-048B 2-4 in 11/5/2002 2-4" 0.036 U 0.036 U NDYRDTS-049A 0-2 in 11/5/2002 0-2" 0.056 0.043 U 0.056YRDTS-049B 2-4 in 11/5/2002 2-4" 0.036 U 0.036 U NDYRDTS-050A 0-2 in 11/5/2002 0-2" 0.1 0.036 U 0.1YRDTS-050B 2-4 in 11/5/2002 2-4" 0.046 0.036 U 0.046YRDTS-051A 0-2 in 11/11/2002 0-2" 0.097 0.036 U 0.097YRDTS-051B 2-4 in 11/11/2002 2-4" 0.036 U 0.58 0.58

YRDTS-023

YRDTS-024

YRDTS-037

YRDTS-038

YRDTS-031

YRDTS-032

YRDTS-033

YRDTS-034

YRDTS-035

YRDTS-036

YRDTS-051

YRDTS-043

YRDTS-044

YRDTS-045

YRDTS-047

YRDTS-048

YRDTS-049

YRDTS-050

YRDTS-046

YRDTS-039

YRDTS-040

YRDTS-041

YRDTS-042

Page 2 of 6



SB0010006I 10/23/2003 0-6" 0.04 U 0.045 0.045SB0010203F 10/22/2003 2-3' 0.04 U 0.097 0.097

SB002 SB0020006I 10/22/2003 0-6" 0.19 0.14 0.33SB003 SB0030006I 10/22/2003 0-6" 0.04 U 0.31 0.31SB004 SB0040006I 10/22/2003 0-6" 0.13 0.043 U 0.13SB005 SB-005 0006I 9/7/2004 0-6" 0.035 U 0.035 U NDSB006 SB-006 0006I 9/7/2004 0-6" 0.035 U 0.035 U NDSB007 SB-007 0006I 9/7/2004 0-6" 0.036 U 0.036 U NDSB008 SB0080006I 10/22/2003 0-6" 0.15 0.086 0.24SB009 SB0090006I 10/20/2003 0-6" 0.053 0.053 U 0.053SB010 SB-010 0006I 9/7/2004 0-6" 0.034 U 0.034 U ND

SB-011 0006I 9/7/2004 0-6" 0.038 U 0.038 U NDFD001-090704 9/7/2004 0-6" 0.037 U 0.037 U ND

SB012 SB0120006I 10/22/2003 0-6" 0.016 J 0.046 U 0.016SB013 SB0130006I 10/20/2003 0-6" 0.14 0.05 U 0.14

SB-014 0006I 9/8/2004 0-6" 0.19 0.038 U 0.19SB-014 0203F 9/8/2004 2-3' 0.038 U 0.038 U NDSB-015 0006I 9/9/2004 0-6" 0.038 U 0.038 U NDSB-015 0203F 9/9/2004 2-3' 0.038 U 0.038 U NDSB016-0006I 10/19/2004 0-6" 0.18 0.033 U 0.18SB016-0203F 10/19/2004 2-3' 0.39 0.033 U 0.39SB-017 0006I 9/9/2004 0-6" 1.1 0.038 U 1.1SB-017 0203F 9/9/2004 2-3' 0.037 U 0.037 U NDSB0180006I 10/22/2003 0-6" 0.092 U 0.092 U NDSB0180203F 10/22/2003 2-3' 0.026 J 0.043 U 0.026

SB019 SB0190006I 10/20/2003 0-6" 0.52 0.056 U 0.52SB020 SB0200006I 10/22/2003 0-6" 0.4 0.18 0.58

SB-021 0006I 9/9/2004 0-6" 0.17 0.037 U 0.17SB-021 0203F 9/9/2004 2-3' 0.040 U 0.040 U NDSB0220006I 10/20/2003 0-6" 0.68 0.046 U 0.68

FD202102003 10/20/2003 0-6" 1.1 0.043 U 1.1SB0220102F 10/20/2003 1-2' 0.04 U 0.04 U NDSB-023 0006I 9/8/2004 0-6" 0.037 U 0.82 0.82SB-023 0203F 9/8/2004 2-3' 0.035 U 0.035 U NDSB024-0006I 10/7/2004 0-6" 0.041 U 0.041 U NDSB024-0203F 10/7/2004 2-3' 0.038 U 0.038 U ND

SB025 SB0250006I 10/22/2003 0-6" 0.043 U 0.043 U NDSB0260006I 10/22/2003 0-6" 1.1 0.58 1.68SB0260203F 10/22/2003 2-3' 0.12 0.059 U 0.12SB0270006I 10/22/2003 0-6" 0.096 0.036 U 0.096SB0270102F 10/22/2003 1-2' 0.12 0.067 0.187

SB028 SB0280006I 12/2/2004 0-6" 0.033 U 0.033 U NDSB029-0006I 10/19/2004 0-6" 0.32 0.033 U 0.32SB029-0203F 10/19/2004 2-3' 0.28 0.033 U 0.28

SB030 SB0300006I 10/22/2003 0-6" 0.48 0.2 0.68SB031 SB0310006I 10/22/2003 0-6" 0.18 0.043 U 0.18SB032 SB0320006I 10/20/2003 0-6" 2.5 0.23 U 2.5

SB0330006I 10/22/2003 0-6" 0.57 0.25 0.82SB0330102F 10/22/2003 1-2' 0.15 0.036 U 0.15SB-034 0006I 9/8/2004 0-6" 0.036 0.035 U 0.036SB-034 0203F 9/8/2004 2-3' 0.038 U 0.038 U NDSB0350006I 10/20/2003 0-6" 0.53 0.35 0.88SB0350102F 10/20/2003 1-2' 0.033 U 0.033 U NDSB-036 0006I 9/8/2004 0-6" 0.63 0.67 1.3SB-036 0203F 9/8/2004 2-3' 0.036 U 0.036 U NDSB037-0006I 10/7/2004 0-6" 0.097 0.034 U 0.097SB037-0203F 10/7/2004 2-3' 0.036 U 0.036 U NDSB037-0910F 10/7/2004 9-10' 0.038 U 0.038 U NDSB0380006I 12/2/2004 0-6" 0.033 U 0.033 U NDSB0380203F 12/2/2004 2-3' 0.033 U 0.033 U NDSB0380506F 12/2/2004 5-6' 0.033 U 0.033 U NDSB039-0006I 10/24/2004 0-6" 0.033 U 0.033 U NDSB039-0203F 10/24/2004 2-3' 0.033 U 0.033 U NDSB040-0006I 12/1/2004 0-6" 0.033 U 0.091 0.091SB040-0203F 12/1/2004 2-3' 0.033 U 0.033 U ND

SB035

SB036

SB037

SB039

SB038

SB027

SB029

SB033

SB034

SB022

SB023

SB024

SB026

SB016

SB017

SB018

SB021

SB001

SB011

SB014

SB015

SB040

Page 3 of 6



SB041-0006I 10/7/2004 0-6" 0.15 0.035 U 0.15SB041-0203F 10/7/2004 2-3' 0.54 0.035 U 0.54

FD-007-100704 10/7/2004 2-3' 0.36 0.037 U 0.36SB041-1415F 10/7/2004 14-15' 0.036 U 0.036 U NDSB042-0006I 10/24/2004 0-6" 2.6 0.066 U 2.6SB042-0203F 10/24/2004 2-3' 0.66 0.033 U 0.66SB042-0510F 10/24/2004 5-10' 0.062 0.033 U 0.062SB-044 0006I 9/8/2004 0-6" 0.60 0.24 0.84SB-044 0203F 9/8/2004 2-3' 0.034 U 0.034 U NDSB-045 0006I 12/2/2004 0-6" 14 0.33 U 14SB-045 0203F 12/2/2004 2-3' 0.033 U 0.033 U NDSB046 0006I 9/30/2004 0-6" 1.4 0.035 U 1.4SB046 0506F 9/30/2004 5-6' 0.92 0.034 U 0.92SB-047-0006I 9/30/2004 0-6" 0.082 0.036 U 0.082SB-047-0203F 9/30/2004 2-3' 0.4 0.038 U 0.4SB0480006I 10/30/2003 0-6" 0.12 0.07 0.19SB0480203F 10/30/2003 2-3' 0.21 0.11 0.32SB0490006I 10/30/2003 0-6" 0.26 0.11 0.37SB0490203F 10/30/2003 2-3' 0.036 U 0.036 U NDSB050-0006I 10/24/2004 0-6" 0.082 0.058 0.14SB050-0203F 10/24/2004 2-3' 0.047 0.033 U 0.047

SB051 SB051-0006I 10/16/2004 0-6" 0.037 U 0.037 U NDSB052 SB052-0006I 12/2/2004 0-6" 0.033 U 0.033 U ND

SB-053 0006I 9/8/2004 0-6" 0.32 0.036 U 0.32SB-053 0203F 9/8/2004 2-3' 0.034 U 0.034 U NDSB0540006I 10/30/2003 0-6" 0.033 U 0.033 U NDSB0540203F 10/30/2003 2-3' 0.033 U 0.033 U NDSB0541415F 10/30/2003 14-15' 0.033 U 0.033 U NDSB-056 0006I 9/7/2004 0-6" 0.057 0.036 U 0.057SB-056 0203F 9/7/2004 2-3' 0.036 U 0.036 U NDSB-056 1415F 9/7/2004 14-15' 0.035 U 0.035 U ND

SB057 SB0570006I 10/30/2003 0-6" 0.036 J 0.043 U 0.036SB0580006I 10/30/2003 0-6" 0.35 0.19 0.54SB0580203F 10/30/2003 2-3' 0.036 U 0.036 U NDSB0580506F 10/30/2003 5-6' 0.049 0.036 U 0.049

SB059 SB0590006I 10/30/2003 0-6" 0.033 U 0.033 U NDSB0600006I 10/30/2003 0-6" 0.19 0.12 0.31SB0600203F 10/30/2003 2-3' 0.2 0.14 0.34

SB063 SB-063 1415F 9/30/2004 14-15' 0.035 U 0.035 U NDSB064 SB064-1415F 9/30/2004 14-15' 0.036 U 0.036 U NDSB065 SB065 1415F 9/30/2004 14-15' 0.036 U 0.036 U NDSB073 SB073-00006I 11/16/2004 0-6" 0.033 U 0.033 U ND

SB-074 0006I 9/8/2004 0-6" 0.034 U 0.13 0.13SB-074 0203F 9/8/2004 2-3' 0.035 U 0.035 U ND

SB075A SB075A-0006I 12/1/2004 0-6" 0.033 U 0.033 U NDSB075A-0203F 12/1/2004 2-3' 0.12 0.033 U 0.12

SB075B SB075B-0006I 12/2/2004 0-6" 0.096 0.033 U 0.096SB075B-0203F 12/2/2004 2-3' 0.084 0.033 U 0.084

SB076 SB0760006I 12/2/2004 0-6" 1.6 0.13 U 1.6SB0780006I 9/27/2004 0-6" 0.52 0.037 U 0.52

SB0780006I A 10/6/2004 0-6" 2.1 0.075 U 2.1SB0780006I B 10/6/2004 0-6" 0.24 0.037 U 0.24SB0780006I C 10/6/2004 0-6" 1.1 0.039 U 1.1SB0780616I A 10/6/2004 6-16" 0.089 0.038 U 0.089SB0780616I C 10/6/2004 6-16" 0.2 0.038 U 0.2SB0780618I B 10/6/2004 6-18" 0.063 0.039 U 0.063

SB079 SB079-0006I 10/24/2004 0-6" 0.033 U 0.033 U NDSB080 SB080-0006I 11/30/2004 0-6" 0.035 0.033 U 0.035SB081 SB081-0006I 12/2/2004 0-6" 0.042 0.033 U 0.042SB082 SB0820006I 12/2/2004 0-6" 0.045 0.033 U 0.045SB084 SB084-0006I 10/16/2004 0-6" 0.064 0.036 U 0.064SB085 SB085-0006I 10/19/2004 0-6" 0.033 U 0.033 U NDSB086 SB086-0006I 10/17/2004 0-6" 0.20 0.034 U 0.20

SB0870006I 10/20/2003 0-6" 0.11 0.074 0.18SB0870102F 10/20/2003 1-2' 0.051 0.036 U 0.051SB0880006I 10/20/2003 0-6" 0.083 0.04 U 0.083SB0880102F 10/20/2003 1-2' 0.036 U 0.036 U ND

SB078

SB087

SB088

SB056

SB058

SB060

SB074

SB049

SB050

SB053

SB054

SB046

SB047

SB048

SB045

SB041

SB042

SB044

Page 4 of 6



SB0890006I 10/20/2003 0-6" 0.3 0.4 0.7SB0890102F 10/20/2003 1-2' 0.033 U 0.033 U NDSB0900006I 10/20/2003 0-6" 0.51 0.033 U 0.51SB0900102F 10/20/2003 1-2' 0.12 0.036 U 0.12

SB091 SB091-0006I 10/24/2004 0-6" 0.033 U 0.033 U NDSB092-0006I 10/17/2004 0-6" 0.034 U 0.034 U NDSB092-0203F 10/17/2004 2-3' 3.1 0.071 U 3.1SB092-1415F 10/17/2004 14-15' 0.037 U 0.037 U NDSB093-0006I 10/17/2004 0-6" 0.040 U 0.040 U ND

FD-008-101704 10/17/2004 0-6" 0.040 U 0.040 U NDSB093-0203F 10/17/2004 2-3' 0.035 U 0.035 U NDSB093-1415F 10/17/2004 14-15' 0.037 U 0.037 U NDSB094-0006I 10/20/2004 0-6" 0.046 0.033 U 0.046SB094-0203F 10/20/2004 2-3' 0.033 U 0.033 U NDSB095-0006I 10/24/2004 0-6" 0.067 0.033 U 0.067SB095-0203F 10/24/2004 2-3' 0.033 U 0.033 U NDSB095-1213F 10/24/2004 12-13' 0.033 U 0.033 U NDSB-096 0006I 9/9/2004 0-6" 0.035 U 0.035 U NDSB-096 0203F 9/9/2004 2-3' 0.038 U 0.038 U ND

SB097 SB097-0006I 11/30/2004 0-6" 0.26 0.033 U 0.26SB100 SB1000006I 10/21/2003 0-6" 0.13 0.1 0.23

SB1010006I 10/21/2003 0-6" 0.036 J 0.04 U 0.036SB1010102F 10/21/2003 1-2' 0.014 J 0.04 U 0.014

SB102 SB1020006I 10/21/2003 0-6" 0.35 0.27 0.62SB103 SB1030006I 10/21/2003 0-6" 0.034 J 0.056 U 0.034

SB1040006I 10/21/2003 0-6" 0.059 0.04 U 0.059FD203102103 10/21/2003 0-6" 0.018 J 0.036 U 0.018SB1050006I 10/21/2003 0-6" 0.036 U 0.036 U NDSB1050102F 10/21/2003 1-2' 0.04 U 0.04 U ND

SB106 SB1060006I 10/21/2003 0-6" 0.15 0.04 U 0.15SB107 SB1070006I 10/21/2003 0-6" 0.084 0.036 U 0.084SB108 SB1080006I 10/21/2003 0-6" 0.036 U 0.036 U ND

SB1090006I 10/20/2003 0-6" 0.036 U 0.036 U NDSB1090102F 10/20/2003 1-2' 0.036 U 0.036 U ND

SB110 SB1100006I 10/20/2003 0-6" 0.094 0.036 U 0.094SB111 SB1110006I 10/20/2003 0-6" 0.05 0.036 U 0.05SB112 SB1120006I 10/20/2003 0-6" 0.036 U 0.036 U ND

SB1130006I 10/20/2003 0-6" 0.044 0.04 U 0.044FD-201102003 10/20/2003 0-6" 0.035 J 0.04 U 0.035

SB1140006I 10/16/2003 0-6" 0.033 U 0.033 U NDSB1140203F 10/16/2003 2-3' 0.036 U 0.036 U NDSB1150006I 10/16/2003 0-6" 0.036 U 0.036 U NDSB1150102F 10/16/2003 1-2' 0.036 U 0.036 U NDSB1160006I 10/15/2003 0-6" 0.036 U 0.036 U NDSB1160203F 10/15/2003 2-3' 0.04 U 0.04 U NDSB1170006I 10/15/2003 0-6" 0.043 U 0.043 U NDSB1170102F 10/15/2003 1-2' 0.043 U 0.043 U NDSB1180006I 10/15/2003 0-6" 0.036 U 0.036 U NDSB1180102F 10/15/2003 1-2' 0.043 U 0.043 U ND

SB122 SB1221415F 10/28/2003 14-15' 0.036 U 0.036 U NDSB123 SB1230304F 10/28/2003 3-4' 0.036 U 0.036 U NDSB124 SB1240910F 10/28/2003 9-10' 0.036 U 0.036 U NDSB125 SB1250910F 10/28/2003 9-10' 0.033 U 0.033 U NDSB126 SB1260203F 10/28/2003 2-3' 0.029 J 0.04 U 0.029SB127 SB1270102F 10/28/2003 1-2' 0.13 0.036 U 0.13SB128 SB12800061 10/14/2003 0-6" 0.036 U 0.036 U NDSB129 SB12900061 10/14/2003 0-6" 0.033 0.033 U 0.033

SB115

SB116

SB117

SB118

SB105

SB109

SB113

SB114

SB096

SB101

SB094

SB104

SB090

SB092

SB093

SB095

SB089

Page 5 of 6



SB130 SB13000061 10/14/2003 0-6" 0.046 0.036 U 0.046SB131 SB13100061 10/14/2003 0-6" 0.033 U 0.033 U ND

SB1390006I 10/23/2003 0-6" 0.04 U 0.04 U NDSB1390203F 10/23/2003 2-3' 0.036 U 0.036 U NDSB1410006I 10/16/2003 0-6" 0.036 U 0.036 U NDSB1410203F 10/16/2003 2-3' 0.028 J 0.036 U 0.028SB1430006I 10/16/2003 0-6" 0.036 U 0.036 U NDSB1430102F 10/16/2003 1-2' 0.12 0.036 U 0.12SB1450006I 10/23/2003 0-6" 0.036 U 0.036 U NDSB1450102F 10/23/2003 1-2' 0.033 U 0.033 U ND

FD206102303 10/23/2003 1-2' 0.033 U 0.033 U NDSB1520006I 10/15/2003 0-6" 0.055 0.036 U 0.055SB1520102F 10/15/2003 1-2' 0.036 U 0.036 U NDSB1530006I 10/15/2003 0-6" 0.046 0.04 U 0.046SB1530203F 10/15/2003 2-3' 0.033 U 0.033 U NDSB1540006I 10/15/2003 0-6" 0.044 0.036 U 0.044SB1540102F 10/15/2003 1-2' 0.036 U 0.036 U ND

SB156 SB1560006I 10/28/2003 0-6" 0.036 U 0.036 U NDSB157 SB1570006I 10/28/2003 0-6" 0.036 U 0.036 U NDSB158 SB1580006I 10/28/2003 0-6" 0.036 U 0.036 U NDSB159 SB1590006I 10/28/2003 0-6" 0.036 U 0.036 U ND

SB904-0001F 11/7/2004 0-1' 0.10 0.033 U 0.10SB904-0102F 11/7/2004 1-2' 0.048 0.033 U 0.048SB908-0001F 11/7/2004 0-1' 0.46 0.033 U 0.46SB908-0102F 11/7/2004 1-2' 0.033 U 0.033 U NDSB908-0203F 11/7/2004 2-3' 0.13 0.033 U 0.13SB912-0001F 11/7/2004 0-1' 0.20 0.033 U 0.20SB912-0102F 11/7/2004 1-2' 3.5 0.130 U 3.5SB912-0203F 11/7/2004 2-3' 0.19 0.033 U 0.19SB913-0001F 11/7/2004 0-1' 0.033 U 0.033 U NDSB913-0102F 11/7/2004 1-2' 0.033 U 0.033 U NDSB913-0203F 11/7/2004 2-3' 2.1 0.170 U 2.1SB914-0001F 11/7/2004 0-1' 0.047 0.033 U 0.047SB914-0102F 11/7/2004 1-2' 0.033 U 0.033 U NDSB914-0203F 11/7/2004 2-3' 0.084 0.034 U 0.084SB915-0001F 11/7/2004 0-1' 0.045 0.033 U 0.045SB915-0102F 11/7/2004 1-2' 0.033 U 0.033 U NDSB915-0203F 11/7/2004 2-3' 0.033 U 0.033 U NDSB916-0001F 11/7/2004 0-1' 0.14 0.033 U 0.14SB916-0102F 11/7/2004 1-2' 0.15 0.033 U 0.15SB916-0203F 11/7/2004 2-3' 0.084 0.033 U 0.084SB917-0001F 11/7/2004 0-1' 0.033 U 0.033 U NDSB917-0102F 11/7/2004 1-2' 0.033 U 0.033 U NDSB917-0203F 11/7/2004 2-3' 0.033 U 0.033 U NDSB918-0304F 11/7/2004 3-4' 0.033 U 0.033 U NDSB918-0405F 11/7/2004 4-5' 0.033 U 0.033 U NDSB918-0506F 11/7/2004 5-6' 0.033 U 0.033 U NDSB918-0607F 11/7/2004 6-7' 0.033 U 0.033 U NDSB918-0708F 11/7/2004 7-8' 0.033 U 0.033 U NDSB918-0809F 11/7/2004 8-9' 0.033 U 0.033 U NDSB918-0910F 11/7/2004 9-10' 0.033 U 0.033 U NDAlley 1-0 in 11/22/2004 0" 2.3 0.072 U 2.3Alley 1-6 in 11/22/2004 6" 2 0.170 U 2Alley 2-0 in 11/22/2004 0" 2.3 0.170 U 2.3Alley 2-6 in 11/22/2004 6" 1.5 0.066 U 1.5Alley 3-0 in 11/22/2004 0" 6.1 0.170 U 6.1Alley 3-6 in 11/22/2004 6" 2.1 0.170 U 2.1Alley 4-0 in 11/22/2004 0" 1.8 0.170 U 1.8Alley 4-6 in 11/22/2004 6" 0.48 0.033 U 0.48Alley 5-0 in 11/22/2004 0" 0.36 0.033 U 0.36Alley 5-6 in 11/22/2004 6" 0.2 0.033 U 0.2Alley 6-0 in 11/22/2004 0" 0.42 0.033 U 0.42Alley 6-6 in 11/22/2004 6" 4 0.170 U 4

PAB-001 PAB-001-0006I 10/16/2004 0-6" 0.29 0.039 U 0.29PAB-002 PAB-002-0006I 10/16/2004 0-6" 0.16 0.046 U 0.16PAB-003 PAB-003-0006I 10/16/2004 0-6" 0.47 0.036 U 0.47

PAB-005-0006I 10/16/2004 0-6" 0.91 0.036 U 0.91PAB-005-0612I 10/16/2004 6-12" 0.036 U 0.036 U NDPAB-005-1218I 10/16/2004 12-18" 0.12 0.036 U 0.12

Notes:U= Below Detection LimitJ= Estimated Value

Alley 6

PAB-005

Alley 1

Alley 2

Alley 3

Alley 4

SB915

SB916

SB917

Alley 5

SB918

SB908

SB912

SB913

SB914

SB152

SB153

SB154

SB904

SB139

SB141

SB143

SB145

Page 6 of 6

Table 2Soil PCB Analytical Program Yankee Nuclear Power StationRowe, MA

Study Area PCB Comment

Study Area 1 SB1001 0003I XSB1001 0102F XSB1001 0203F XSB1001 0304F XSB1002 0003I XSB1002 0102F XSB1002 0203F XSB1002 0304F XSB1003 0003I XSB1003 0102F XSB1003 0203F XSB1003 0304F XSB1004 0003I XSB1004 0102F XSB1004 0203F XSB1004 0304F XSB1005 0003I XSB1005 0102F XSB1005 0203F XSB1005 0304F XSB1006 0003I XSB1006 0102F XSB1006 0203F XSB1006 0304F XSB1006 0406F XSB1006 0608F XSB1007 0003I XSB1007 0102F XSB1007 0203F XSB1007 0304F XSB1008 0003I XSB1008 0102F XSB1008 0203F XSB1008 0304F XSB1009 0003I XSB1009 0102F XSB1009 0203F XSB1009 0304F XSB1010 0003I XSB1010 0102F XSB1010 0203F XSB1010 0304F XSB1011 0003I XSB1011 0102F XSB1011 0203F XSB1011 0304F XSB1012 0003I XSB1012 0102F XSB1012 0203F XSB1012 0304F XSB1012 0406F XSB1012 0608F XSB1013 0003I X Note depth below original gradeSB1013 0102F XSB1013 0203F XSB1013 0304F XSB1014 0003I X Note depth below original gradeSB1014 0102F XSB1014 0203F XSB1014 0304F X

Field Sample ID

Page 1 of 7


Study Area PCB CommentField Sample ID

Study Area 1 SB1015 0003I X Note depth below original gradeSB1015 0102F XSB1015 0203F XSB1015 0304F XSB1016 0003I X Note depth below original gradeSB1016 0102F XSB1016 0203F XSB1016 0304F XSB1017 0003I X Note depth below original gradeSB1017 0102F XSB1017 0203F XSB1017 0304F XSB1018 0003I X Note depth below original gradeSB1018 0102F XSB1018 0203F XSB1018 0304F XSB1019 0003I X Note depth below original gradeSB1019 0102F XSB1019 0203F XSB1019 0304F XSB1020 0003I X Note depth below original gradeSB1020 0102F XSB1020 0203F XSB1020 0304F XSB1021 0003I X Note depth below original gradeSB1021 0102F XSB1021 0203F XSB1021 0304F XSB1022 0003I XSB1022 0102F XSB1022 0203F XSB1022 0304F XSB1022 0406F XSB1022 0608F XSB1023 0003I XSB1023 0102F XSB1023 0203F XSB1023 0304F XSB1024 0003I XSB1024 0102F XSB1024 0203F XSB1024 0304F XSB1025 0003I XSB1025 0102F XSB1025 0203F XSB1025 0304F XSB1025 0406F XSB1025 0608F XSB1026 0003I XSB1026 0102F XSB1026 0203F XSB1026 0304F XSB1027 0003I XSB1027 0102F XSB1027 0203F XSB1027 0304F X

Page 2 of 7



Study Area 1 SB1028 0003I XSB1028 0102F XSB1028 0203F XSB1028 0304F XSB1029 0003I XSB1029 0102F XSB1029 0203F XSB1029 0304F XSB1030 0003I XSB1030 0102F XSB1030 0203F XSB1030 0304F XSB1031 0003I XSB1031 0102F XSB1031 0203F XSB1031 0304F XSB1032 0003I XSB1032 0102F XSB1032 0203F XSB1032 0304F XSB1033 0003I XSB1033 0102F XSB1033 0203F XSB1033 0304F XSB1034 0003I XSB1034 0102F XSB1034 0203F XSB1034 0304F XSB1035 0003I XSB1035 0102F XSB1035 0203F XSB1035 0304F XSB1036 0003I XSB1036 0102F XSB1036 0203F XSB1036 0304F XSB1037 0003I XSB1037 0102F XSB1037 0203F XSB1037 0304F XSB1038 0003I XSB1038 0102F XSB1038 0203F XSB1038 0304F X

Study Area 2 SB1039 0003I XSB1040 0003I XSB1041 0003I XSB1042 0003I XSB1043 0003I XSB1044 0003I XSB1044 0102F XSB1045 0003I XSB1046 0003I XSB1047 0003I XSB1048 0003I XSB1049 0003I XSB1050 0003I XSB1051 0003I X

Page 3 of 7



Study Area 2 SB1052 0003I XSB1052 0102F XSB1053 0003I XSB1054 0003I XSB1055 0003I XSB1056 0003I X Replicate of YRDTS-020SB1057 0003I XSB1058 0003I XSB1059 0003I X Replicate of YRDTS-043SB1060 0003I XSB1060 0102F XSB1061 0003I XSB1061 0102F XSB1062 0003I XSB1062 0102F XSB1063 0003I XSB1063 0102F XSB1064 0003I XSB1064 0102F XSB1065 0003I XSB1065 0102F XSB1066 0003I XSB1066 0102F XSB1067 0003I XSB1068 0003I XSB1069 0003I XSB1069 0102F XSB1069 0203F HSB1069 0304F HSB1070 0003I XSB1070 0102F XSB1070 0203F HSB1070 0304F HSB1071 0003I XSB1071 0102F XSB1071 0203F HSB1071 0304F HSB1072 0003I XSB1072 0102F XSB1072 0203F HSB1072 0304F H

Study Area 3 SB1073 0003I XSB1073 0102F XSB1073 0203F HSB1073 0304F HSB1074 0003I XSB1074 0102F XSB1074 0203F HSB1074 0304F HSB1075 0003I XSB1075 0102F XSB1075 0203F HSB1075 0304F HSB1076 0003I XSB1076 0102F XSB1076 0203F HSB1076 0304F H

Page 4 of 7



Study Area 3 SB1077 0003I XSB1077 0102F XSB1077 0203F HSB1077 0304F HSB1078 0003I XSB1078 0102F XSB1078 0203F HSB1078 0304F HSB1079 0003I XSB1079 0102F XSB1079 0203F HSB1079 0304F HSB1080 0003I XSB1080 0102F XSB1080 0203F HSB1080 0304F HSB1081 0003I XSB1081 0102F XSB1081 0203F HSB1081 0304F HSB1082 0003I XSB1082 0102F XSB1082 0203F HSB1082 0304F HSB1083 0003I XSB1083 0102F XSB1083 0203F HSB1083 0304F HSB1084 0003I XSB1084 0102F XSB1084 0203F HSB1084 0304F HSB1085 0003I XSB1085 0102F XSB1085 0203F HSB1085 0304F HSB1086 0003I XSB1086 0102F XSB1086 0203F HSB1086 0304F HSB1087 0003I XSB1087 0102F XSB1087 0203F HSB1087 0304F HSB1088 0003I XSB1088 0102F XSB1088 0203F HSB1088 0304F HSB1089 0003I XSB1089 0102F XSB1089 0203F HSB1089 0304F HSB1090 0003I XSB1090 0102F XSB1090 0203F HSB1090 0304F H

Page 5 of 7



Study Area 3 SB1091 0003I XSB1091 0102F XSB1091 0203F HSB1091 0304F HSB1092 0003I X Replicate of SB092SB1092 0102F XSB1092 0203F HSB1092 0304F H

Study Area 4 SB1093 0003I XSB1093 0102F XSB1093 0203F HSB1093 0304F HSB1094 0003I X All depths tested to delineate vertical extentSB1094 0102F XSB1094 0203F XSB1094 0304F XSB1094 0406F XSB1094 0608F XSB1095 0003I XSB1095 0102F XSB1095 0203F HSB1095 0304F HSB1096 0003I XSB1096 0102F XSB1096 0203F HSB1096 0304F HSB1097 0003I XSB1097 0102F XSB1097 0203F HSB1097 0304F HSB1098 0003I XSB1098 0102F XSB1098 0203F HSB1098 0304F HSB1099 0003I XSB1099 0102F XSB1099 0203F HSB1099 0304F HSB1100 0003I XSB1100 0102F XSB1100 0203F HSB1100 0304F HSB1101 0003I XSB1101 0102F XSB1101 0203F HSB1101 0304F HSB1102 0003I XSB1102 0102F XSB1102 0203F HSB1102 0304F HSB1103 0003I XSB1103 0102F XSB1103 0203F HSB1103 0304F HSB1104 0003I XSB1104 0102F XSB1104 0203F HSB1104 0304F H

Page 6 of 7



SB1105 0003I XSB1105 0102F XSB1105 0203F HSB1105 0304F HSB1106 0003I XSB1106 0102F XSB1106 0203F HSB1106 0304F HSB1107 0003I XSB1107 0102F XSB1107 0203F HSB1107 0304F HSB1108 0003I XSB1108 0102F XSB1108 0203F HSB1108 0304F HSB1109 0003I XSB1109 0102F XSB1109 0203F HSB1109 0304F HSB1110 0003I XSB1110 0102F XSB1110 0203F HSB1110 0304F HSB1111 0003I XSB1111 0102F XSB1112 0003I XSB1112 0102F XSB1113 0003I XSB1113 0102F XSB1114 0003I XSB1114 0102F XSB1115 0003I X Replicate of SB026SB1115 0102F X

Notes:X - PCB sample to be testedH - PCB sample to be held

Study Area 5

Page 7 of 7

Scale 1:25,000

0 1,000 ft0.5 mi

0 500 m0.5 km

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SITE

Figure 1 - Locus MapYankee Nuclear Power Station - Rowe, MA

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Appendix A Gradient Memorandum on Sampling

Appendix B Rapid Assay Instructions

STRATEGIC DIAGNOSTICS INC.

RaPID Assay PCB Test Kit A00133/A00134

Intended Use NOTE: Color development is inversely proportional to

the PCB concentration. The RaPID Assay PCB Test Kit can be used as a quantitative, semi-quantitative or qualitative enzyme immunoassay (EIA) for the analysis of PCB (polychlorinated biphenyl) in water (groundwater, surface water, well water). For applications in other matrices please contact our Technical Service department or refer to the soil application procedure provided. The RaPID Assay PCB Test Kit allows reliable and rapid screening for PCB (measured and reported as Aroclor 1254), with quantitation between 0.5 and 10 ppb (as Aroclor 1254), in water. The minimum detection level of the kit is 0.2 ppb (as Aroclor 1254.)

Darker color = lower concentration Lighter color = higher concentration The determination of the PCB level in an unknown sample is interpreted relative to the standard curve generated from kit standards after reading with a spectrophotometer. Performance Characteristics The PCB RaPID Assay will detect different PCB Aroclors to different degrees. Refer to the table below for data on several of these. The PCB RaPID Assay kit provides screening results. As with any analytical technique (GC, HPLC, etc. ) positive results requiring some action should be confirmed by an alternative method.

Test Principles

The PCB RaPID Assay kit applies the principles of enzyme linked immunosorbent assay (ELISA) to the determination of PCB and related compounds. The sample to be tested is added, along with an enzyme conjugate, to a disposable test tube, followed by paramagnetic particles with antibodies specific to PCB attached. Both PCB (which may be in the sample) and the enzyme labeled PCB (the enzyme conjugate) compete for antibody binding sites on the magnetic particles. At the end of an incubation period, a magnetic field is applied to hold the paramagnetic particles (with PCB and labeled PCB analog bound to the antibodies on the particles, in proportion to their original concentration) in the tube and allow the unbound reagents to be decanted. After decanting, the particles are washed with Washing Solution.

The PCB RaPID Assay immunoassay test does not differentiate between PCB and other related compounds. The table below shows compounds at the method detection limit (MDL) which is the lowest concentration of the compound, in water, that can be picked up in the assay. The limit of quantitation (LOQ) is an approximate concentration, in water, required to yield a positive result at the lowest standard. This is the lowest concentration of the compound that can be quantified in the assay. The IC50 is the concentration required to, inhibit one half of the color produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

The presence of PCB is detected by adding the enzyme substrate (hydrogen peroxide) and the chromogen (3,3’,5,5’ – tetramethylbenzidine). The enzyme labeled PCB analog bound to the PCB antibody catalyzes the conversion of the substrate/chromogen mixture to a colored product. After an incubation period, the reaction is stopped and stabilized by the addition of acid. Since the labeled PCB (conjugate) was in competition with the unlabeled PCB (sample) for the antibody sites, the color developed is inversely proportional to the concentration of PCB in the sample.

Compound MDL

(ppb) LOQ (ppb)

IC50 (ppb)

Aroclor 1254 0.20 0.50 3.6 Aroclor 1260 0.20 0.32 2.3 Aroclor 1248 0.22 0.59 4.22 Aroclor 1242 0.34 1.22 8.8 Aroclor 1262 0.36 0.66 4.74 Aroclor 1232 0.84 2.61 18.76

2 RaPID Assay PCB Test Kit

Aroclor 1268 0.92 3.03 21.80 Aroclor 1016 0.94 3.56 25.60 Aroclor 1221 13.54 22.58 162.60 *The following compounds demonstrated no reactivity in the PCB RaPID Assay test kit at concentrations up to 10,000 ppb: Biphenyl, 2,5-Dichlorophenol, 2,3,5-Trichlorophenol, Di-n-octyl-phthalate. The presence of the following substances up to 250 ppm were found to have no significant effect on PCB RaPID Assay results: copper, nickel, zinc, mercury, manganese, phosphate, sulfate, sulfite, magnesium, calcium, nitrate and thiosulfate. Humic acid up to 25 ppm and iron to 100 ppm were found to have no significant effect. In addition, sodium chloride concentrations up to 1.0 M showed no effect on results. Precautions • Training is strongly recommended prior to using the

RaPID Assay test system. Contact Strategic Diagnostics for additional information.

• Treat PCB, solutions that contain PCB, and potentially contaminated samples as hazardous materials.

• Use gloves, proper protective clothing, and methods to contain and handle hazardous material where appropriate.

• Reagents must be added in a consistent manner to the

entire rack. A consistent technique is the key to optimal performance. Be sure to treat each tube in an identical manner.

• Water samples should be at a neutral pH prior to

analysis. Samples containing gross particulate should be filtered (e.g. 0.2 um AnotopTM 25 Plus, Whatman, Inc.) to remove particles.

• Store all test kit components at 2°C to 8°C (36°F to 46°F). Storage at ambient temperature (18°C to 27°C or 64°F to 81°F) on the day of use is acceptable. Test tubes require no special storage and may be stored separately to conserve refrigerator space.

• Allow all reagents to reach ambient temperature (18°C

to 27°C or 64°F to 81°F) before beginning the test. This typically requires at least 1 hour to warm from recommended storage conditions.

• Do not freeze test kit components or expose them to temperatures above 100°F (39°C).

• Do not use test kit components after the expiration

date.

• Do not use reagents or test tubes from one test kit with reagents or test tubes from a different test kit.

• Do not mix reagents from kits of different lot numbers.

• Use approved methodologies to confirm any positive results.

• Do not under any circumstances attempt to

disassemble the base of the magnetic rack. Magnets will be violently attracted to each other.

• Adequate sample number and distribution are the

responsibility of the analyst.

• The photometer provided in the accessory kit requires electricity and comes with a 110V adapter. Adapters for 220V are available. Do not attempt to operate with a car adaptor.

• Do not expose color solution to direct sunlight. • Do not dilute or adulterate test reagents or use

samples not called for in the test procedure; this may give inaccurate results.

• Tightly recap the standard vials when not in use to

prevent evaporative loss. Materials Provided

• Antibody Coupled Paramagnetic Particles in buffered saline containing preservative and stabilizers.

30 test kit: one 20 mL vial 100 test kit: one 65 mL vial

• Enzyme Conjugate.


• Standards

Three concentrations (0.25, 1.0 and 5.0 ppb) of PCB standards (as Aroclor 1254) in buffered saline

RaPID Assay PCB Test Kit 3

containing preservative and stabilizers are supplied. Each vial contains 4 mL.

• Control

A concentration (approximately 3 ppb) of PCB (as Aroclor 1254) in buffered saline containing preservative and stabilizers. A 4 mL volume is supplied in one vial.

• Diluent/Zero Standard

Buffered saline containing preservative and stabilizers without any detectable PCB.


• Color Solution containing hydrogen peroxide and 3,3’,5,5’-tetramethylbenzidine in an organic base. 30 test kit: one 20 mL vial 100 test kit: one 65 mL vial

• Stop Solution containing a solution of 2M sulfuric

acid. 30 test kit: one 20 mL vial 100 test kit: one 60 mL vial

• Washing Solution containing preserved deionized water.


• Polystyrene test tubes

30 test kit: one 36 tube box 100 test kit: three 36 tube boxes

• User’s Guide

Materials Required and Ordered Separately See “Ordering Information” for the appropriate catalogue numbers.

Rapid Assay Accessory Kit

Accessory equipment may be rented or purchased from Strategic Diagnostics. See “Ordering Information” for the appropriate catalogue numbers. The accessory kit contains the following items:

• Adjustable Volume Pipet

• EppendorfTM Repeater® Pipettor

• Electronic timer

• Portable balance capable of weighing 10 g (for soil samples)

• Vortex mixer

• Magnetic separation rack

• RPA-I RaPID Analyzer (or equivalent spectrophotometer capable of reading 450 nm in a 1 mL sample size).

Other Items • 12.5 mL Combitips® for the Repeater pipettor - for

0.25 mL to 1.25 mL dispensing volumes (5)

• Pipet tips for adjustable volume pipet (100-1000 uL) NOTE: Order replacement Combitips® and pipet tips

separately. See the “Ordering Information" section.

Materials Required but Not Provided • Methanol (HPLC grade or equivalent) – for water analysis

• Protective clothing (e.g., latex gloves)

• Absorbent paper for blotting test tubes

• Liquid and solid waste containers

• Marking pen

• Instructional video (optional)

Suggestions for Pipettor Use • Practice using both pipettes (adjustable volume and

Repeater pipettor) with water and extra tips before you analyze your samples.

• Use a new tip each time you use the Repeater pipettor to pipette a different reagent to avoid reagent cross-contamination. Tips can be rinsed thoroughly, dried completely and reused. By using the same tip to dispense the same reagent each time you can avoid cross contamination.

NOTE: Repeator tips should be changed periodically (after ~10 uses) since precision deteriorates with use.


• Draw the desired reagent volume into the Repeater pipettor and dispense one portion of the reagent back into the container to properly engage the ratchet mechanism. If you do not do this, the first volume delivered may be inaccurate.

• To add reagents using the Repeater pipettor, pipette down the side of the test tube just below the rim.

• When adding samples and standard using the positive displacement pipettor, always pipette into the bottom of the tube without touching the sides or bottom of the tube.

• Use a new adjustable volume pipet tip each time you pipette a new unknown.

Assay Procedure Prior to performing your first Rapid Assay, please take time to read the package inserts in their entirety and review the videotape if available. On site training is strongly recommended for new users of this test system. Please contact your account manager for further information. This procedure is designed for quantitative analysis. For running the kit semi-quantitatively or qualitatively, please contact Technical Support.

Collect/Store the Sample The following steps explain how to properly collect and store your samples.

1. Water samples should be collected in glass vessels with teflon cap liners). Immediately upon collection, water samples should be diluted with an equal volume (1:1) of methanol (HPLC grade) to prevent adsorptive losses to the glass containers. This is a 2x dilution, which must be accounted for when interpreting results. See “Results Interpretation”, Section 3a for further details. Use this diluted sample as “sample” in “Perform the Test”.

NOTE: This 2x dilution is not required for soil samples.

2. Samples should be collected in appropriately sized and labeled containers.

3. If testing soil samples, follow the SDI Sample Extraction Kit User’s Guide or the appropriate technical bulletin to properly collect and store your sample.

4. Samples should be tested as soon as possible after collection. If this is not possible, storage at 4°C (39°F) is recommended to minimize evaporative losses.

Set Up 1. Remove kits from refrigerator. All reagents must be

allowed to come to room temperature prior to analysis. Remove reagents from packaging and place at room temperature at least 1 hour prior to testing.

2. Turn on the RPA-1 or other spectrophotometer. The RPA-1 should be warmed up for at least 30 minutes prior to the run.

3. Label five 12.5 mL Combitips “Conjugate”, “Particles”, “Wash”, “Color” and “Stop”. In addition, add the name of the compound you are testing for to each Combitip.

4. Remove nine clean blank test tubes for standards and

control and one test tube for each sample (if testing in singlicate). Label the test tubes according to contents as follows.

Tube # Contents 1 Negative control (replicate 1) 2 Negative control (replicate 2) 3 Standard 1 (replicate 1) 4 Standard 1 (replicate 2) 5 Standard 2 (replicate 1) 6 Standard 2 (replicate 2) 7 Standard 3 (replicate 1) 8 Standard 3 (replicate 2)

9 Control 10 Sample 1 11 Etc.

*Label at top of tubes to avoid interference with reading of tubes in photometer

Sample Extraction, Filtration and Dilution

Filtration may be necessary to remove gross particulate from the water sample. If testing samples at levels higher than standard kit level is desired, contact SDI for special instructions. Water samples should be diluted 1:1 in methanol as described in “Collect/Store the Sample”. Please follow the instructions from the SDI Sample Extraction Kit to prepare and dilute the soil extract prior to running the assay.


Perform the Test 1. Separate the upper rack from the magnetic base. Place

labeled test tubes into the rack. 2. Add 200 uL of standards, control or samples to the

appropriate tubes using the adjustable volume pipet with the dial set on 0200. The negative control, standards and control must be run with each batch of samples.

NOTE: Sample should be added to the bottom of the tube by inserting the pipet tip into the tube without touching the sides or the bottom of the tube. Take care not to contact sample with pipette tip once dispensed into bottom of the tube. 3. Using the Repeater Pipettor with the “Conjugate” tip

attached and the dial set on “1”, add 250 uL of Enzyme conjugate down the inside wall of each tube. (Aim the pipet tip ¼” to ½” below the tube rim or tube wall; deliver liquid gently to avoid splashback.)

4. Thoroughly mix the magnetic particles by swirling

(avoid vigorous shaking) and attach the “Particles” tip to the Repeater Pipettor. With the dial set on “2” add 500 uL of magnetic particles to each tube, aiming down the side of the tube as described above. Vortex, mixing each tube 1 to 2 seconds at low speed to minimize foaming. Pipetting of magnetic particles should be kept to 2 minutes or less.

5. Incubate 15 minutes at room temperature. 6. After the incubation, combine the upper rack with the

magnetic base and press all tubes into the base; allow 2 minutes for the particles to separate.

7. With the upper rack and magnetic base combined, use

a smooth motion to invert the combined rack assembly over a sink and pour out the tube contents.

NOTE: If the rack assembly inadvertently comes apart when lifting to pour out tube contents, re-combine and wait an additional 2 minutes to allow particles to separate. 8. Keep the rack inverted and gently blot the test tube

rims on several layers of paper towels. It is important to remove as much liquid as possible but do not bang the rack or you may dislodge the magnetic particles and affect the results.

9. Set the Repeater Pipettor dial to “4” and put on the tip

labeled “Wash”. Add 1 mL of Washing Solution down the inside wall of each tube by using the technique described earlier. Vortex tubes for 1-2 seconds. Wait 2 minutes and pour out the tube contents as described previously. Repeat this step one more time.

NOTE: The number of washes and wash volume are important in ensuring accurate results. 10. Remove the upper rack (with its tubes) from the

magnetic base. With the “Color” tip attached to the Repeater Pipet and the dial set to “2” add 500 uL of Color Reagent down the inside wall of each tube as described previously. Vortex 1 to 2 seconds (at low speed).

11. Incubate 20 minutes at room temperature. During this

period, add approximately 1 mL of Washing solution to a clean tube for use as an instrument blank for “Results Interpretation”.

12. After the incubation, position the Repeater pipettor at

Setting “2” and use the “Stop” tip to add 500 uL of Stop solution to all test tubes.

13. Proceed with results interpretation. WARNING: Stop solution contains 2M sulfuric

acid. Handle carefully. Results Interpretation 1. After addition of Stop Solution to the test tubes,

results should be read within 15 minutes. 2. Wipe the outside of all antibody coated tubes prior to

photometric analysis to remove fingerprints and smudges.

Photometric Interpretation Using the RPA-I

1. The RPA-I photometer (provided in the Rapid Assay Accessory kit) can be used to calculate and store calibration curves. It is preprogrammed with various RaPID Assay protocols. For the PCB RaPID Assay test kit, parameter settings are as follows:

Data Reduct : Lin. Regression


Xformation : Ln/LogitB

Read Mode : Absorbance

Wavelength : 450 nm

Units : PPB

# Rgt Blk : 0

Calibrators:

# of Cals : 4

# of Reps : 2

Concentrations:

#1: 0.00 ppb

#2: 0.25 ppb

#3: 1.00 ppb

#4: 5.00 ppb

Range : 0.10 – 5.00

Correlation : 0.990

Rep. %CV : 10%

NOTE: Prior to analysis the RPA-I User’s Manual should be thoroughly reviewed for more detailed operation instructions.

2. Follow the instrument prompts to read the absorbance

of all tubes:

Instrument Display Operator Response SELECT COMMAND Press RUN RUN PROTOCOL Scroll using the YES []

or NO [] keys until the desired protocol appears. Then press ENTER

SPL. REPLICATES (1-5) Press 1 (for analysis of samples in singlicate.) Press ENTER

BLANK TUBE, Insert blank tube INSERT TUBE, containing 1mL wash EVALUATING TUBE, solution. REMOVE TUBE (Beep) Remove tube CAL #1, REP. #1, Insert Tube #1 INSERT TUBE,

EVALUATING TUBE, REMOVE TUBE (Beep) Remove tube

Follow prompts to read tubes. NOTE: Tube order is important. The RPA-I expects to

see the standards in ascending order, in duplicate, starting with the negative control.

Following evaluation of all standards, the instrument will display:

PRINTING DATA, Data will print PRINTING CURVE Curve will print only if

programmed to print (See RPA1 User’s Manual).

CTRL #1 REP #1, Insert Control Tube INSERT TUBE, EVALUATING TUBE, REMOVE TUBE (Beep) Remove Tube EDIT CALIBRATORS Press NO (if editing is YES/NO necessary press YES and refer to the RPA1 User’s Manual). SPL #1 REP#1 Insert first sample tube INSERT TUBE EVALUATING TUBE REMOVE TUBE (Beep) Remove tube

Continue to follow prompts. After all samples have been read, press STOP. Expected Results: • %CV (coefficient of variation) between standard

duplicates of 10% or less.

• Absorbance reading for the 0 ppb standard should be between 0.8 and 2.0 for all assays.

• Correlation (r) of 0.990 or greater for all assays.

• Kit control within range specified on vial.

• Absorbance of negative control and standards should be as follows:

Negative Control>Std. 1>Std. 2>Std. 3.


3. Concentrations will be indicated for all samples on the RPA-I printout.

a) The concentration, as indicated on the

printout, is multiplied by the appropriate dilution factor (if applicable) introduced in the procedure. The quantitation range of the kit is also multiplied by this factor.

EXAMPLE: Water samples were diluted 2-fold with methanol upon collection (see “Collect/Store the Sample” in this User’s Guide). As a result, the concentrations listed on the printout should be multiplied by 2 to determine the sample concentration. The standard concentrations are also multiplied by 2 to give a quantitation range in water for this test kit of 0.5 to 10 ppb.

b) Samples with an “nd” and no concentration

listed have an absorbance greater than the negative control; therefore, no concentration can be computed for these samples. Results must be reported as < 0.5 ppb (or Standard 1 multiplied by the dilution factor.)

c) Samples with an “nd” next to a listed

concentration have an estimated concentration below the minimum detection level of the test kit. Results must be reported as <0.5 ppb (or Standard 1 multiplied by the dilution factor.)

NOTE: Any samples with concentrations determined to be lower than Standard 1 (the limit of quantitation) must be reported as < 0.5 (or Standard 1 multiplied by the dilution factor.) Quantitation is not possible below this standard as this is outside the linear range of the assay.

d) Similarly, samples with a “hi” next to a listed

concentration have an estimated concentration higher than Standard 3 and must be reported as >10 ppb (or Standard 3 multiplied by the dilution factor.)

NOTE: In order to determine the concentration of samples with concentrations greater than Standard 3, they must be subjected to repeat testing using a diluted sample. A ten-fold or greater dilution of the sample is recommended with an appropriate amount of PCB diluent. This additional dilution must then be

taken into account when calculating the concentration. Please contact technical support for assistance in performing dilutions. Photometric Interpretation Using Other Photometers Other photometers may also be used to interpret results obtained from the RPA-I photometer. It is important that the photometer be able to read absorbance at 450nm and that the instrument can read at a 1 mL fill volume. Absorbances obtained from other spectrophotometers (reading at 450 nm) may be used to manually calculate sample concentrations as outlined below. 1. Calculate the mean absorbance for each of the three

standards and the negative control. 2. Determine the standard deviation and %CV

(coefficient of variation) of each standard and ensure %CV is less than 10% for each.

3. Calculate the %B/Bo for each standard by dividing the mean absorbance value for the standard by the mean absorbance value for the negative control and multiplying the results by 100.

4. Construct a standard curve by plotting the %B/Bo for

each standard on the vertical logit (y) axis versus the corresponding analyte concentration on the horizontal logarithmic (x) axis on the graph paper provided in the test kit. Graph papers are specific for each method. Use only the graph paper supplied with each kit.

5. Draw the best straight line through all points. Using

the %B/Bo of the sample, the concentration can be interpolated from the standard curve.

6. Multiply results by the appropriate dilution factor (if

applicable) introduced in the procedure. For example, if the sample was diluted 10-fold to increase the detection levels of the kit then the results must be multiplied by 10. This dilution also changes the range of the assay (standards) by the same factor.

NOTE: Do not forget to account for the 2x dilution introduced in the “Collect/Store the Sample” procedure for water samples.


Limitations of the Procedure

The Rapid Assay PCB Test Kit is a screening test only. Sampling error may significantly affect testing reliability. Adequate sample number and distribution are the responsibility of the analyst.

RaPID Assay PCB Test Kit 9 Ordering Information

Description Catalogue Number Rapid Assay PCB Kit A00133/A00134 Rapid Assay Accessory Kit** 6050100 Adjustable Volume Pipet Tips (100-1000 uL) A00013 12.5 mL Combitip for Repeating Pipette (1 each) A00009 PCB Diluent A00136 PCB Soil Proficiency Sample A00175 Rapid Assay Accessory Kit Rental 6997010

** To obtain part numbers and pricing for individual items in the Accessory Kit contact SDI at the number below. Ordering/Technical Assistance

Should you have any questions regarding this procedure prior to analysis contact Technical Service to avoid costly mistakes. To Place an Order or Receive Technical Assistance, please call Strategic Diagnostics Inc. at: Call toll-free 800-544-8881` Or 302-456-6789 Phone 302-456-6782 Fax Web site: www.sdix.com E-mail: [email protected] General Limited Warranty

SDI’s products are manufactured under strict quality control guidelines and are warranted to be free from defects in materials and workmanship. New instruments and related non-expendable items are warranted for one year from date of shipment against defective materials or workmanship under normal use and service. Warranty obligation is limited to repair or replacement of the defective product or to refund of the purchase price, at the discretion of SDI. Other warranties, express or implied, are disclaimed. SDI’s liability under any warranty claim shall not exceed the refund of the purchase price paid by the customer. Under no circumstances shall SDI be liable for special, indirect or consequential damages. Safety To receive an MSDS for this product, visit our web site at www.sdix.com. Copyright© 1997, Strategic Diagnostics Inc., Z00245.1, Rev 4/4/00

http://www.sdix.com/

mailto:[email protected]

Operation of the Repeater Pipet To Set or Adjust Volume To determine the pipetting volume, the dial setting (1-5) is multiplied by the minimum pipetting volume of the tip (indicated on the side of the Combitip, e.g. 1~100 uL.) To Assemble Pipet Tip Slide filling lever down until it stops. Then raise the locking clamp and insert the tip until it clicks into position. Be sure the tip plunger is fully inserted into the barrel before lowering the locking clamp to affix the tip in place. To Fill Tip With tip mounted in position on pipet, immerse end of tip into solution. Slide filling lever upward slowly. Combitip will fill with liquid. To Dispense Sample Check the volume selection dial to ensure pipetting volume. Place tip inside test tube so that tip touches the inner wall of tube. Completely depress the pipetting lever to deliver sample. NOTE: Dispense one portion of reagent back into the container to engage the ratchet mechanism and ensure accuracy. To Eject Tip Empty tip of any remaining solution into appropriate container by pushing filling lever down. Raise locking clamp upward, and remove the Combitip.

Operation of the Adjustable Volume Pipet To Set or Adjust Volume Press release button on side of pipette and turn the push-button to adjust volume up or down. Volume setting is displayed on top of pipet. See kit instructions for appropriate setting. Pipet will accurately dispense volumes between 100 and 1000 uL. To Assemble Pipet Tip Gently push nose cone of pipet firmly into a pipet tip contained in the pipet tip rack. To Withdraw Sample Keep pipet almost vertical. With tip mounted in position on pipet, press push-button to 1st stop and hold it. Place tip at bottom of liquid sample and slowly release push-button to withdraw measured sample. Ensure that no air bubbles exist in the pipette tip. If bubbles exist, dispense sample and re-withdraw. Slide tip out along the inside of the vessel. To Dispense Sample Wipe any liquid from outside of tip taking care not to touch orifice. Place tip into tube, almost to the bottom, and slowly press push-button to 2nd stop. Hold push-button at 2nd stop when removing tip from tube. To Eject Tip Press push-button to 3rd stop. Tip is ejected.

Appendix C Results Comparison between NEL Off Site Lab Analysis and Rapid Assay Immunoassay PCB Soil Sample Analysis

\202073\workplans\ Memo_sample size with known CVs

Memorandum

To: From: Subject:

John McTigue, Gregg Demers David Merrill Sample Size Issues

Date:

February 7, 2005

The MADEP (1995) Guidance for Disposal Site Risk Characterization provides general suggestions for the number of samples needed for evaluating background conditions, but does not provide any concrete recommendations relating to the number of samples needed to characterize a site (or exposure unit), or the number necessary for risk-based decision-making. This memorandum provides a statistically-based method to estimate the number of samples needed to estimate a population mean with a defined level of confidence. Note that the mean was chosen because under the MCP it is the mean concentration that is used in risk calculations, and hence the mean concentration is a target for cleanup level decisions. While there are a number of alternative statistical methods that address sample size issues, many of them require an a priori estimate of the variance of the data. As described in Gilbert (1987), the sample size required at a defined level of confidence (α) can be defined in terms of the coefficient of variation (CV) and the relative difference (RD) about the mean:

= −

RDCVZN 2/1 α

where Z1-α/2 = Standard Normal variate associated with α confidence (α is the cumulative area

under the standard normal probability curve) CV = σ/µ , or the coefficient of variation RD = | x -µ| /µ , or the relative difference about the sample mean and true mean. Using the historical data collected, the CV is calculated using the following formula:

=

meanStdCV

where: Std = Standard deviation of the mean In evaulating the Polychlorinated Biphenyl (PCBs) data from historical sampling, two Aroclors (Aroclor 1254 and Aroclor 1260) are detected in soil. Further examination of the data indicated the PCB data for

20 University Road, Cambridge, MA 02138 • (617) 395-5000 • Facsimile (617) 395-5001 • www.gradientcorp.com

both aroclors are log normalized. Therefore, in order to more accurately calculate the CV, the log normalized data is used in the calculation:

=

)()(

meanLnStdLnCV

where: Ln(Std) = Log normalized standard deviation of the log normalized mean; Ln(mean)= Log normalized mean The arithmetic CV of all the PCB data is 3.4. The CV of the log-transformed data is 1.1. When sampling over "exposure units" of 1 to 2 acres, it is reasonable to expect a CV to be on the order of 1.0 to 2.0. Although the acceptable value of the relative difference about the mean is a risk-management decision, U.S. EPA's Soil Screening Guidance (1996) indicates that the sample mean can be compared to twice the Soil Screening Level (e.g., 2×SSL) when making risk-based decisions. This implies a relative difference of 1.0 as being an acceptable level of accuracy. On this basis, in order to have reasonable assurance (e.g., 90% to 95%) that the sample mean will be within ± 1-fold of the true mean requires up to 25 samples for situations where a CV of up to 2.5 is expected (as shown below, the number of samples is less than 25 for CV<2.5). Thus if the true mean at the site is 1 ppm, then collecting 25 samples should be adequate at a 95% probability of being within ± 1 ppm of the true mean. Because this analysis is based on the relative difference about the true mean and sample mean, it can be applied to any range of environmental monitoring data of interest. The approach does not require information about the absolute value of the sample and true mean.

Table 1 Sample Sizes Required for Estimating the Mean (µ)

with Associated Relative Difference (RD) About the Mean Coefficient of Variation (CV)

Confidence (1-α) Relative

Difference (RD) 0.5 1.0 1.5 2.0 2.5

0.95 0.5 4 16 35 62 97Z0.975 = 1.96 1 1 4 9 16 25

0.90 0.5 3 11 25 44 68

Z0.95 = 1.645 1 1 3 7 11 17

Note that the number of samples needed to define the mean with an acceptable tolerance is independent of the area to be sampled. However, as a practical matter, if there is wide variation in the concentration measurements found in environmental media, this can give rise to very large CV values, and in turn lead to an increase in the number of samples needed to estimate the mean within the specified tolerance. One approach that can address this issue is to subdivide large sites into smaller investigative units, or exposure

\202073\workplans\ Memo_sample size with known CVs 2 Gradient CORPORATION

units, within which the concentration may exhibit less variation (e.g., smaller CV within these sub-units). This type of stratified sampling is one way of potentially avoiding the wide variation in concentration (e.g., large CV) that may occur if a large site is considered a single investigative unit.



References Gilbert, R. O. 1987. Statistical Methods for Environmental Pollution Monitoring. John Wiley & Sons, New York. MADEP. 1995. Guidance for Disposal Site Risk Characterization. Interim Final Policy WSC/ORS-95-141. USEPA. 1996. "Soil screening guidance: Technical background document." NTIS PB96-963502; EPA-540/R-95/128; OSWER Publication 9355.4-17A. Office of Solid Waste and Emergency Response. May 1996.

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PCB Soil Field Sampling Plan - Yankee Rowe (PCB) Soil Field Sampling Plan (FSP) for the Yankee Nuclear Power Station ... of PCB-containing paint chips. Approximately 3,500 cubic yards