ESDR I JSID I SID Abstracts

1354 VARIEGATE PORPHYRIA: IDENTIFICATION OF 10 NOVEL MUTATIONS IN THE MOLECULAR HETEROGENEITY IN ERYTHROPOIETIC PROTOPORPHYRIA:

10 MUTATIONS IN THE FERROCHELATASE GENE. Fmnk K. Jugerl’, Joroe

1352 PHOTOPROTECTIVE EFFECTS OF OLEANOLIC ACID AND URSODEOXYCHOLIC

ACID ON THE CHRONIC ULTRAVIOLET RADIATION li’,DUCED CUTANEOUS

DAMAGES Sane Tae Kim, Kee Zuck S& Youne SW Chae. Hvun Cheol Kim

De,,atment of Dermatolow Kosln Mfxhcal College, Pusan, Korea

We have previously demonstrated that the expression of UVA-mduced collagenax

and elastin mRNA was reduced by all-lrans-retinoic acid (t-RA) and ursalic acid

Although oleanolic acid(OA) and ursodeoxychobc acxd(UDCA) have been suggested as

phatopratectants that can reduce and improve the acute as well as chronic skin

damage because of their chemical or bmlagical similarities with steroids. their

antiox,dati”e effect and immunomodulatory properties, the effect of OA and UDCA on

the collagenase and elasbn synthesis by UVA irradiation IS unknown. The possibility

uf inhibition of UVA-induced collagenase and &&in synthesis by 0.4 as well as

UDCA was invest&wed in this study. Confiuently cultured human d-al fibroblasts

were rrradiated with 15J/cm2 of UVA. OA and UDCA were administered and kept m

culture media for 24 hours before or after UVA irmdiatmn. Total RNA was isolated

fallowing UVA irradiatkx and subjected Lo northern blot analysis using oligolabelled

cDNA for collagenase and elaetin. The result was as fallows. The exprrssion of

UVA-induced collagenase and elashn mRNA was reduced when OA and UDCA were

admuastercd before or after u-radiation. Tb,r result suggesh that OA and ““CA may

lba”v photowotitx”e effect on photoagmg “la inhibition of UVA-induced collagenasc

and &&in svnthesm

1355

1356 THE EFFECT OF SUN EXPOSURE ON THE DELIVERY AND UTlLLZATlON OF OXYGEN IN SKIN. - Weinkauf and Uma Santhanam. Unilever Research, U.S., Edgewater, NJ.

Photoaged skin is a result of frequent and cumulative photodamage superimpwed on intrinsic aging. It is characteri&d by rough texture; laxity, w&l&, irregular pigmentation and capillary changes. Little is known about the metabolic changes in skin caused by sun exposure. The present study was initiated to investigate the effect of photodamage on oxygen delivery and utilization in facial skin. The study population consisted of 41 women with various levels of photodamage on the face. Oxygenated hemoglobin levels were measured using a diffuse reflectance spectrophotomda (DRS) to determine oxygen delivery. Oxygen utilization was assessed indirectly using a transcutaneous monitor to measure oxygen and carbon dioxide levels at the surface of the skin. In addition, the amount of blood flow was mdirectly estimated via the milliwatts feature of the transcutaneous monitor. Measurements were taken on the face and compared to a photoprotected site i.e. inner, uo~er arm. No significant differences were observed in milliwatts or oxxenated h&oglobin with &reasing facial photodamage. However, transcutaneou~ oxygen levels were lower (~~0.08) on the facial skin of subiects with mild photodamage compared to moderate photodamage. These results suggest that facial phbtodamageis accompanied by a decrease in skin’s capability to consume oxygen, without an apparent effect on delivery of oxygen to skin. Dramatic differences were observed between measurements taken on the face and on the inner, upper arm. Transcutaneous oxygen levels on facial skin were much lower than on the inner, upper arm and milliwatts and oxygenated hemoglobin measurements were higher on the face than on the arm. These results suggest that blood flow and oxygen utilization xe much higher

in facial skin relative to arm skin.