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7/26/2019 Phng Php Bo Tn Ngun Gene Thc Vt
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PHNG PHP BO TN NGUN GEN THC VTc vit ngy T! "i# $% T&ng '
()$* )+,$-
Ti ng.y/n 0i t1.y2n t3c v4t c5 6 ng7" 8."n t19ng t1:ng vi;c c.ng
ci;. ?@i
A. c: c&c cng t1Dn c9n t: giFng# Ac i;t > c&c ng.In
gJn c5 ?K nLng cFng cM. . ;n
i# c&c Ai2. ?i;n ng:i cKn i cng n nQng =RS cng nWng ngi;= v
=&t
t1in n2n nWng ngi;= 2n vQng# g5= =n 8."n t19ng v: cWng c.Xc Y5"
A5i giKS ngZ:# "n t:n >ng
t3c v K: v; SWi t1[ng c\" t:n c.
C&c ]c c\ y. c\" K: tIn ng.In gJn t3c v4t
C&c =ng =&= K: tIn ti ng.y/n 0i t1.y2n t3c v4t,
BK: tIn nXi vi ^in_it.`
BK: tIn nXi vi, > 0.y t1D c&c 8.n t t3c v4t t1:ng Ai2.
?i;n t3 ni/n ni Y.:i cy A5 Ng.IngJn t3c v4t Ac K: tIn @ nWng t1i#
v[n gi" ADn :c t1/n AIng 1.Xng C&c >:i cy >S ngi;= v
cy :"ng 0i t[ng Ac t: c&c vang K: tIn t3 ni/n n v[n 8.Fc gi"
:c ?. K: tIn HDn
t!c K: tIn nXi vi gIS,
_ BK: tIn t1/n nWng t1i
_ BK: tIn t1:ng v[n gi" ADn
_ BK: tIn cy >S ngi;= v cy :"ng 0i @ ?. K: v; :c v[n 8.Fc
gi"
BK: tIn nXi vi Ac c:i > SXt Ac ta A 0.y t1D c&c 8.n t in
0M t1:ng SWi t1[ng c"n t&c :c SWi
t1[ng t3 ni/n c\" cbng# c: == 8.& t1Dn tin 5" t3 ni/n YKy
1"
BK: tIn nXi vi c5 nQng ti2S nLng t1:ng vi;c K: tIn 8.& t1Dn
tdc ngi c\" t3c v4t v]i SWi t1[ngFng t3 ni/n c\" cbnge K: tIn A"
0ng in 9c @ S9i S!c AX _ ; in t&i# >:i v 0]i >:ie gfn
?t
cXng AIng v]i ngn ng gJn t1:ng vi;c K: tIn v 0hng ng.In gJne c:
== c&c ng.In gJn v t1i
t!c Kn AM" Ac 0hng# =&t t1in v in Ai ^PS TM jn# ())k`
Vi;t N"S > SXt t1:ng nQng 8.Fc gi" A. ti/n @ c. Ac t"S gi" v:
SXt F 03 &n ngi/n c!. c
@ ?:" 9c v Yy 03ng SW Dn K: tIn nXi vi @ S!c t:n c. tl nQng nLS
+) c\" t ?m ^PS
TM jn# ())k`
http://thongtinkhcn.binhthuan.gov.vn/index.php/tin-t-c-va-s-ki-n/tin-khcn-d-a-phuong/153-phuong-phap-b-o-t-n-ngu-n-gen-th-c-v-thttp://thongtinkhcn.binhthuan.gov.vn/index.php/tin-t-c-va-s-ki-n/tin-khcn-d-a-phuong/153-phuong-phap-b-o-t-n-ngu-n-gen-th-c-v-t
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7/26/2019 Phng Php Bo Tn Ngun Gene Thc Vt
2/7
BK: tIn ng:i vi ^JY_it.`
BK: tIn ng:i vi > A" ng.In gJn 1" ?oi Ai2. ?i;n t3 ni/n in
Fng c\" cbng :c 1" ?oi ; tFng
Kn Y.:i Kn AM"#p
Nc AiS, Ci =d tFn ?S# Ari oi t1Dn AX ?s t.4t cWng ng; c":e BK:
tIn c\ y. 0hng t1:ng
tng >"i
Png t!c v ?s t.4t K: tIn ng:i vi =h t.Xc v: >:i cy t1Ing# i;n
n"y c5 % =ng =&= K:
tIn ?&c n". gIS
_ Ngn ng gJn t ": gIS ngn ng t @ c&c c 8."n K: tIn v ngn ng
t cXng AIng
_ Ngn ng gJn AIng 1.Xng
_ BK: tIn in_vit1: v]i cK n5S cy t1Ing ?t t v n5S cy t1Ing in Kn
in 0ng# ci" tn "i
>:i K: tIn t :SW v K: tIn t =n
_ V[n t3c v4t
Ngn ng gJn >. giQ# 0.y t1D v t&i in t1@ >i c&c Sw.
Fng c\" giFng cy t1Ing Kn AM"# giFng AM"
=ng# giFng cKi tin# cy :"ng 0i v 9 ng :"ng 0i Ng.In gJn t1:ng
c&c ngn ng gJn AKS
K: c\ng cF vQng cfc ng.In c.ng cng t3c# t3c =RS v n. c. ?&c
c: c:n ng[i# Kn
Y."i
BK: tIn in_vit1:
BK: tIn ng:i vi Ac &= 0hng 1Xng 1xi n K: tIn ngn ng gJn# ngn
ng gJn AIng 1.Xng# nngcng c5 St n c n:i cy ?Wng cM. v]i 8.&
t1Dn >S ?W# nQng >:i in
Kn in 0ng Ngn ng gJn AIng 1.Xng t.4n ti;n c: vi;c A&n gi
0hng# nng t[ng g=
1\i 1: . ;n# t[i tit i u: A5# K: tIn in_vit1: > =ng =&=
.ng v t"y t A ?fc =hc
nQng n c t1/n# c: == ?iS t1" 3 c ;n t1]c ?i K: tIn v c5 t K: tIn
F >ng >]n
^V VLn it# ())+`
. AiS,
_ KS K: AX "n t:n v c ;n c":# c5 ?K nLng t: 8.n t cy AIng nng
>]n
_ V]i =ng =&= K: 8.Kn i/. >n c5 t K: 8.Kn Ac >. 0i v]i
F >ng >]n v AX n AMn
_ Hn c ?K nLng S= >i
BK: tIn in_vit1: &= 0hng v]i "i =ng t!c
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7/26/2019 Phng Php Bo Tn Ngun Gene Thc Vt
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_ Png t!c >S c4S in t1@ng, Sw. ng.In gJn Ac giQ 0]i 0ng SW
t3c v4t :c cy c:n
t1/n SWi t1[ng 0in 0ng Cy in t1@ng c4S c5 t K: tIn ngfn n# t1.ng
n# 0i n
_ Wng >n, Sw. n.Wi cong# =ng t!c ny !ng 0hng c: K: tIn 0i
n
Pn >:i K: tIn in_vit1:
_ BK: tIn 0i n > K: tIn t1:ng nit >ong ^_$+%:C` { ni;t AX
ny# SW nglng tn Ac "tt" 0hng K: tIn t : c 1Ft
nLS $+-$ n n"y ?s t.4t Ax =&t t1in v]i ( ?s t.4t c\ y. >,
?s t.4t t1.y2n tFng 0hng ( ]c
AWng >n c4S c5 .ng cne ?s t.4t AWng >n S]i v]i Ac AiS AWng
>n n"n
?:Kng $))):C =bt |ng c&c nbng t13c ti= v: nit >ong
_ BK: tIn t1.ng n, c&c Ai2. ?i;n n.Wi ci;. K: tInBK: tIn
t1.ng n c5 t c:i > SXt ngn ng gJn# g9i > ngn ng gJn in_vit1:
:t AXng BK: tIn
t1.ng n n.Wi cS c4S c&c gi"i A:n in >6e
_ B .ng t/S t&c nn c4S in t1@ng v: SWi t1[nge
_ Ni;t AX tng ti/n 8."n An ng.In gJn
ri oi =ng =&= ti= c4n# ?s nLng tFt A t3c i;ne c} 2n vQng v
i;. 8.K ?i vi;c K: tIn in
>i c: nWng 0n Tng AFi 0 t3c i;n |ng ngn &c tl c&c
ng.In ?&c n".
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7/26/2019 Phng Php Bo Tn Ngun Gene Thc Vt
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Ng[i K: tIn _ nWng 0n _ t13c ti= @ng >i i n.4n Ac ci" tWng
8." c c ci" >i
n.4n v ti= c4n ng.In gJn T1:ng t3c t c" c5 c c t3c ti n: Ac Yy
03ng v &= 0hng
Mt s vn phng php lun v bo tn ti nguyn di truyn th vt
!hi"n l# bo tn ti nguyn di truyn th vt l $"t h#p hi ho h%i phng
php&'(situ v in(situ) ! n* $inh t" pht tri+n , h-nh thnh .y /
s0 vt ht/% bo tn &'(situ nn %ng 1u%n t23 nhiu "n bo tn in(situ)
4g# l5i6 n* %ng pht tri+n h7 u tin t5o lp # ng2n hng g&n th8h
h#p + gi9 ho$h:ng 3t ngun g&n /% 3-nh nn phi u tin bo tn
&'(situ)))
; 33 , # thng nht + ?nhh*ng hi"n l#ng v phng php bo tn ti nguyn
di truyn th vt)
@) ! ngun g&n AgingB thu d%nh 3C bo tn l + phC vC rng r,i ho
3C tiu v i t#ng sD dCng trong n*) M thng bo tn tinguyn 2y trng 1u
gi% ho5t ng th&o nguyn tJ 3ng l*i do 4g2n hng g&n2y trng 1u
gi% iu phi)
K) Lo tn 1uE g&n .n 3 bo nghi3 ngp = thnh ph.n l ging trng
trOt /% loi v n v? ph2n lo5i At%'%B 2y ho%ng d5i APild
r&l%tiv&sB6 h/ y"u l hi v loi6 =1u%n h> g.n gQi v*i loi
2y =)))
R) Sh t u tin bo tn 1uE g&n loi 2y trng # ' ?nh nh s%uT
( ! loi 2y bn ?%6
( ! loi 2y nhp ni l2u Ui , tr0 thnh 2y trng /% 1u gi%6
?%phng)
( ! ngun g&n 1uV AdonorsB /% ging nhp ni v i ti"n)
W) Sh t u tin bo tn /% tXng loi C th+T
( ! ging ?% phng
( ! ging thng phY3 phF bi"n rng trong sn 'ut
( ! ngun g&n 1uV AdonorsB /% ging nhp ni v dZng hOnging Ui
%o)
[)\hng php bo tnT
\hng php bo tn d% vo phng th sinh sn loi 2yT
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7/26/2019 Phng Php Bo Tn Ngun Gene Thc Vt
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( ! loi 2y sinh sn b]ng h5t ging6 # hi% l3 G nh=3T
^ ! loi 2y = h5t ging d_ t8nh Ah5t ging orthodo'BT Lo tn
&'(situng2n hng g&n h5t ging trong $ho l5nh)
^ ! loi 2y = h5t ging $h= t8nh Ah5t ging r&%ltr%ntB6 thu
nh=3
ny l 2y l2u n`3T Lo tn in(situ hon t nhin)
Lo tn in(situT Lo 1un ng Adyn%3i pr&s&rv%tionB) !2y trng
ti"n hob-nh thUng6 lo5i trX t bi"n $h:ng = l#i) fh:ng bo tn # s
l#ng ging l*n6hi ph8 Jt6 $h= ti"n hnh) != h%i bi"n thT
( Lo tn in(situ p dCng ho loi 2y trng l2u n`3 = h5t ging $h=
t8nhv loi 2y ho%ng d5i hO hng g.n gQi v*i 2y trng)
( Lo tn trn ng rung /% n:ng d2nT p dCng ho loi 2y trng hngn`3
nhng h7 i v*i ging = th+ % vo u sn 'ut v sinh l#i hon:ng d2n)
!hi"n l# bo tn ti nguyn di truyn th vt l $"t h#p hi ho h%i
phngphp &'(situ v in(situ) ! n* $inh t" pht tri+n , h-nh thnh
.y / s0 vtht /% bo tn &'(situ nn %ng 1u%n t23 nhiu "n bo tn
in(situ) 4g# l5i6 n* %ng pht tri+n h7 u tin t5o lp # ng2n hng
g&n th8h h#p + gi9ho $h:ng 3t ngun g&n /% 3-nh nn phi u tin
bo tn &'(situ) !Qng nhnhiu n* %ng pht tri+n $h6 n* t% .n u tin
.u t ho bo tn &'(situ +lu gi9 %n ton v ng`n h
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7/26/2019 Phng Php Bo Tn Ngun Gene Thc Vt
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' ti"n bo tn in(situ + hH tr# ho bo tn &'(situ trong vi>
duy tr- 1u tr-nh ti"nho t nhin /% 2y n:ng nghi>p)
-nh th lu gi9T
u gi9 2y on6 1u%n6 3:6 ph:i6 t" bo6 4 ))) /% ngun g&n
trongiu $i>n duy tr- sinh tr0ng ti thi+u ho3 1u%n6 3: ho
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7/26/2019 Phng Php Bo Tn Ngun Gene Thc Vt
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( != $h n`ng t5o r% bi"n d? o3% v*i t.n s bi"n d? $h nh%u v 8t
l
