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PLANNING AND CONDUCT OF FIELD WORK BY PLANNING AND CONDUCT OF FIELD WORK BY DRC TEAM IN COLLABORATION WITH CONGO DRC TEAM IN COLLABORATION WITH CONGO
PROJECTPROJECT
By José Justin MBIMBI MAYI MUNENEBy José Justin MBIMBI MAYI MUNENE
COMPOSITION OF TEAM IN CONGO PROJETCOMPOSITION OF TEAM IN CONGO PROJET
American team, DRC team and Congo teamAmerican team, DRC team and Congo team
In DRC teamIn DRC team
Tshibwabwa, Tshibwabwa,
Musibono, Musibono,
Monsebula and Mbimbi .Monsebula and Mbimbi .
One of us have a particular work in Congo Project ( WWW//research. One of us have a particular work in Congo Project ( WWW//research. amnh. org/ichthyology/congo/ ). amnh. org/ichthyology/congo/ ).
For myself, I’m a collaborator for field work and actually I make For myself, I’m a collaborator for field work and actually I make researches in Lulua River in collaboration with other members of researches in Lulua River in collaboration with other members of Congo Project.Congo Project.
NEED OF INFORMATION BY AMERICAN TEAM NEED OF INFORMATION BY AMERICAN TEAM FOR SOME CONGO TRIBUTRIES /SUB-FOR SOME CONGO TRIBUTRIES /SUB-
TRIBUTRIESTRIBUTRIES
• they send funds and equipments they send funds and equipments
-cast -net, -cast -net,
-deep- net -deep- net
-rotenone, -rotenone,
-small tube containing pure -small tube containing pure alcohol for alcohol for
DNA samplingDNA sampling
PLANNING AND CONDUCT OF FIELD WORKPLANNING AND CONDUCT OF FIELD WORK
• Before each field trip Before each field trip
- the permission to the University authorities - the permission to the University authorities
- establish a calendar for field work- establish a calendar for field work
Monday wendsday Thursday Saturday Sunday
25 26 27 28 29
19 20 21 22
30 1 2 3 4 5 6
7 8 9 10 11 12 13 7 8 9 10 11 12 13
14 15 16 17 18 19
Légende
Done Kinshasa, June 14th 2008 By José-Justin MBIMBI MAYI MUNENE
Travel to Kananga KanangaKananga
16 Lulua (Katende II)
Sampling Lu Nsan
Travel to Tblu
Sampling to Tblu
Lulua (Katende I)
Return to Kananga
Lulua to bridge pont
Rivière Fwa
Lulua (Beya)
Rivière au choix
Préparation matérielle
Retour à Kinshasa
19
20
25
27
4
TRAVEL TO THE SITE CHOSEN FOR SAMPLINGTRAVEL TO THE SITE CHOSEN FOR SAMPLING
• PROTOCOLPROTOCOL
-the order of mission is presented to the -the order of mission is presented to the
locality chief locality chief
- explain the importance of my research - explain the importance of my research
-the different fishing methods are -the different fishing methods are
presented and explained too. presented and explained too.
FISHERMENFISHERMEN
• choose two or three fishermen who would help us to find the choose two or three fishermen who would help us to find the suitable sampling sites and assist us in catching fishes. suitable sampling sites and assist us in catching fishes.
• Before sampling, I use to begin to present and explain unto Before sampling, I use to begin to present and explain unto them the different methods that they will use for sampling and them the different methods that they will use for sampling and how to do it.how to do it.
• I ask them to explain me :I ask them to explain me :
- which kind of technical they practically use to catch fish, - which kind of technical they practically use to catch fish,
-in which habitat (place) of the river they catch fish -in which habitat (place) of the river they catch fish
- the best period for capturing fish in they site.- the best period for capturing fish in they site.
SAMPLING TECHNICALSAMPLING TECHNICAL
• For sampling, we usually use For sampling, we usually use cast -net (in rain season or dry season)cast -net (in rain season or dry season)
Deep- net ((in rain season or dry season) Deep- net ((in rain season or dry season)
rotenonerotenone ( dry season) ( dry season)
FISHES COLLECTEDFISHES COLLECTED
• For each sampling, the specimens are divided (separated) in two groupsFor each sampling, the specimens are divided (separated) in two groups
-DNA sampling -DNA sampling
- for taxonomic studies)- for taxonomic studies)
• DNA SAMPLINGDNA SAMPLING
Two or four specimens are selected for DNA sampling for Two or four specimens are selected for DNA sampling for
each species.each species.
Each selected specimen is tagged with a unique Each selected specimen is tagged with a unique
plastic label (where we have a number code. plastic label (where we have a number code.
The pelvic fin is taken and preserved in a small tube The pelvic fin is taken and preserved in a small tube
containing pure containing pure alcohol alcohol .Each tube is labelled with the .Each tube is labelled with the
same number as the label which is tagged to the specimen.same number as the label which is tagged to the specimen.
PRESEVATION SPECIMENPRESEVATION SPECIMEN
• The specimens selected and labelled for DNA samples The specimens selected and labelled for DNA samples
plus the specimens for the taxonomic studies are then plus the specimens for the taxonomic studies are then
fixed and preserved in formalin (10%)fixed and preserved in formalin (10%)
SITE INFORMATIONSITE INFORMATION• All informations about geographic coordinates (latitude & longitude) All informations about geographic coordinates (latitude & longitude)
are taken with a GPS, date, name of site are consigned in copy are taken with a GPS, date, name of site are consigned in copy book for work field (See modal)book for work field (See modal)
• Pictures are taken for each site of sampling to give an idea on the Pictures are taken for each site of sampling to give an idea on the aquatic biotope and surrounding habitat (See a modal)aquatic biotope and surrounding habitat (See a modal)
N° Site Geog. Coord. Date Sample name Photo Code DNA 6°14’82’’S
22°28’80 E 30/6/2008 S5E1 Telogramma monogramma Ok 132
Lulua (Kasende)
Telogramma monogramma 133
Telogramma monogramma 134
DESTINATION OF FISFES COLLECTEDDESTINATION OF FISFES COLLECTED
• All fish collected and DNA sample are expedited to All fish collected and DNA sample are expedited to the AMNH for identification.the AMNH for identification.