PLC 2050/2250/2500 Purification Systems - Gilson Guide_Gilson PLC 2050, 2250 and 2… · 2 Tae o Contents | PLC 2050/2250/2500 Purification Systems Table of Contents Table of Contents1

Gilson, Inc. | PO Box 620027 | Middleton, WI 53562-0027 | Tel: 608-836-1551 OR 800-445-7661 | Fax: 608-831-4451

Gilson S.A.S. | 19, avenue des Entrepreneurs | BP 145, F-95400 Villiers-le-bel, FRANCE

www.gilson.com | [email protected] | [email protected] | [email protected]

PLC 2050/2250/2500 Purification SystemsUser’s Guide

2 Table of Contents PLC 2050/2250/2500 Purification Systems|

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General Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

Safety Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8

Liquid Chromatography (LC) Practices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8

Electrical Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9

Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Sécurité . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Consignes Générales de Sécurité . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Symboles Suivants. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Déballage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Chromatographie Liquide (LC) - Aspects Pratiques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Risque électrique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2 General DescriptionPLC 2050/2250/2500 Purification Systems Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3 Detailed Description2-Head Pump. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

2- or 4-Way Low Pressure Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Dynamic Mixing Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Through-Loop Injection Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

UV-VIS Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Fraction Collector. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Touchscreen Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

External Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Unpacking List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

PLC 2050/2250/2500 Purification Systems (Standard). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

PLC 2050/2250/2500 Purification Systems (Optional Accessories) . . . . . . . . . . . . . . . . . . . . . . . 26

3PLC 2050/2250/2500 Purification Systems User’s Guide|

Table of Contents

4 InstallationPlace the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30

Placing the Solvent Trays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30

Install Hydraulic Plumbing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31

Inlet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31

Ballasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32

Valve Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32

Waste . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

Purge Valve Outlet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

Injection Valve Waste Outlet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34

Injection Loop Connection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34

Position the Solvent Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34

Column Set Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35

Prepare the Fraction Collector. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36

Position the Stylus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

Connect the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

5 OptionsManual Loop Injection Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

Column Switching Valve (Manual) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

Acquisition Module for Detectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40

Column Holder Kit for Heavier HPLC Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40

Bar Code Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

Fraction Collector Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

Racks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42

Solvent Level Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42

Solvent Level Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43

4 Table of Contents PLC 2050/2250/2500 Purification Systems|

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Prime the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Automatic Loop Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Manual Loop Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

7 MaintenanceIntroduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Clean the Pistons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Replace Mixing Chamber Piston. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Pump Head Disassembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Replace a Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Replace the Check Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Replace the Detector Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

System Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Lamp Removal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Clean the Flow Cell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Maintenance Schedule. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

A WarrantyGeneral . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

Limited Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Fittings, Unions, and Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Pump. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

Detector. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67


Table of Contents

B PartsPLC Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69

Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69

Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70

Fraction Collector. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71

General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71

Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71

Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72

Electronics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72

Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73


General Safety

Safety

General SafetyRead this section before installing and operating PLC 2050/2250/2500 Purification Systems.

This instrument is intended to be used in a laboratory environment by trained technical personnel.

For safe and proper use of this instrument, both operating and service personnel must follow the instructions contained in this guide when installing, cleaning, and maintaining the instrument. The user should immediately report any problems or safety concerns to the distributor.

The safety precautions described in this chapter must be observed during all phases of operation, service, and repair of the instrument. Failure to comply with these precautions or with specific warnings elsewhere in this user’s guide violates safety standards of design, manufacture, and intended use of the instrument. Gilson assumes no liability for the customer’s failure to comply with these requirements.

PLC 2050/2250/2500 Purification Systems have been certified to safety standards required in Canada, Europe, and the United States. Refer to the rear panel label on the instrument and the Declaration of Conformity document for the current standards to which the instrument has been found compliant.

Safety SymbolsThe following safety notices may appear in this document:

Indicates a potentially hazardous situation which, if not avoided, may result in personal injury.

CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in minor or moderate injury. Indicates a potentially hazardous situation which, if not avoided, may result in equipment damage.

WARNING

NOTICE

8 Safety PLC 2050/2250/2500 Purification Systems|

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ng UnpackingPLC 2050/2250/2500 Purification Systems each weigh approximately 143 lbs. (65 kg) and an additional 22 lbs. (10kg) with packaging. It is recommended that the instrument be moved by more than one person; however, two people are preferable.

Verify that all components from the Unpacking List are included. Any damage to the packing material

or missing elements should be reported to the carrier and vendor immediately. Damage in transit is the responsibility of the carrier. Many carriers must receive a damage claim within seven days of delivery, so report the damage immediately.

Keep the box and packing material for future transport of the instrument.

Do not plug in the instrument if any damage is detected. Powering the instrument in a damaged state may result in serious injury and may damage internal components of the instrument.

Liquid Chromatography (LC) PracticesThis system is designed for chromatographic purposes. It must be operated using appropriate solvents and within specified ranges for pressure, flow rates, and temperatures described in the user’s guide. If it is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

When handling potentially hazardous solvents and samples, it is important to follow standard laboratory safety procedures. Common chemicals used in the laboratory can be toxic, corrosive, flammable, or a combination of these properties.

A list of all safety aspects is outside the scope of this manual but the following points should be considered:

● Ensure adequate ventilation

● Wear eye protection

● Wear gloves when handling chemicals

● Ensure all liquid containers are secure

● No smoking or open flames

● Clean up all spills

Never open a solvent line or valve under pressure. Stop the pump first and let the pressure drop to zero. Wear safety glasses. If a line ruptures or a valve opens under pressure, a potentially hazardous spray of liquid might be generated by the pump.

Depending on the toxicity of solvents used, the system may need to be used in a fume hood.


Electrical Hazards

Electrical HazardsRemoval of protective panels may only be performed by a Gilson representative. When it is necessary to use a non-original power cord plug, make sure the replacement cord adheres to following specifications and local building safety codes.

European Union Model

● Connector A Male type E or type F “Schuko,”16 A

● Connector B Female IEC320/C13, 10 A

● 250 V~, H05VV-F 3G1.0mm²

United States and Canadian Model

● Connector A Male type NEMA 15-5, 15 A

● Connector B Female IEC320/C13, 10 A

● 125 V~, SVT 3x18AWG

Replace blown fuses with fuses of the same size and rating described in the manual. Replace faulty or frayed power cords immediately with the same type and rating. Make sure that voltage sources and line voltage match the value for which the instrument is wired.

Important

To ensure the safety of yourself and your coworkers, observe the following:

● Use a 3-wire mains socket with grounded connection. Grounding is necessary to ensure operator safety and proper operation.

● Secure inflammable solvents.

● The temperature of liquids inside the system must be 77°F (25°C) below the lowest flame temperature of any solvents used.

● When not in use, run a clean solvent through the hydraulic circuits. Do not leave buffer in the system, as it may cause blockages and damage the seals.

● Before moving the instrument, the external connections must be disconnected, the hydraulic circuits rinsed and any inlets or outlets plugged.

10 Safety PLC 2050/2250/2500 Purification Systems|

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Federal Communications Commission (FCC)

This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference which will require the user to correct the interference at his or her own expense.

Any changes or modifications to this equipment not expressly approved by Gilson, Inc. may cause harmful interference and void the FCC authorization to operate this equipment.

Electromagnetic Compatibility (EMC) – Conformite Europeenne (CE)

Refer to the Declaration of Conformity for relevant standards.


Sécurité

Sécurité

Consignes Générales de SécuritéMerci de lire attentivement cette section avant toute installation ou utilisation des Systèmes de Purification PLC 2050/2250/2500.

Cet instrument est destiné à être utilisé dans un environnement de laboratoire par un personnel qualifié.

Pour une utilisation correcte et en toute sécurité de l’instrument, il est nécessaire que le personnel qui utilise et réalise la maintenance de l’instrument, suive les instructions contenues dans ce guide lors de l’installation, du nettoyage et de la maintenance de l’instrument. L’utilisateur doit immédiatement signaler au fournisseur, tout problème relatif aux questions de sécurité.

Les consignes de sécurité suivantes doivent être respectées durant toutes les phases de fonctionnement, d’entretien ou de réparation de l’instrument. Le non-respect de ces précautions ou des avertissements spécifiques mentionnés dans ce guide compromet les normes de sécurité de conception, de fabrication et d’utilisation prévue de l’instrument. Gilson décline toute responsabilité en cas d’incapacité du client à se conformer à ces exigences.

Des Systèmes de Purification PLC 2050/2250/2500 a été certifié conformément aux normes de sécurité en vigueur au Canada, en Europe et aux Etats-Unis. Merci de vous reporter aux indications mentionnées sur le panneau arrière de l’instrument ainsi qu’au document de Déclaration de Conformité aux normes pour lesquelles l’instrument a été déclaré conforme.

Symboles SuivantsLes consignes de sécurité suivantes peuvent apparaître dans ce document:

AVERTISSEMENT (Warning) signale une situation potentiellement dangereuse qui, si elle n’est pas évitée, peut entraîner des blessures graves

CAUTION ATTENTION (Caution) signale une situation potentiellement dangereuse qui, si elle n’est pas évitée, peut entraîner des blessures mineures ou légèresAVIS (Notice) signale une situation potentiellement dangereuse qui, si elle n’est pas évitée, peut entraîner des dommages matériels

WARNING

NOTICE

12 Sécurité PLC 2050/2250/2500 Purification Systems|

Déb

alla

ge

Déballage Chaque Système de Purification PLC 2050/2250/2500 pèse environ 65 kg (143 £) auxquels s’ajoutent 10 kg (22 £) d’emballage. Aussi, il est recommandé d’être au moins 2 personnes pour le déplacer.

Assurez-vous que tous les éléments de la liste de colisage soient (Unpacking List) présents. Tout dommage sur le conditionnement ou élément manquant doit être signalé immédiatement au transporteur ainsi qu’au fournisseur. Les dommages pendant le transport sont de la responsabilité du transporteur. De nombreux transporteurs doivent recevoir une réclamation dans les sept jours suivants la livraison, signalez donc immédiatement tout dommage constaté.

Conservez carton et matériel d’emballage pour les transports ultérieurs de l’instrument.

Ne pas brancher l’appareil si vous constatez un dommage. La mise sous tension d’un instrument endommagé peut entraîner des blessures graves et endommager les composants internes de l’instrument.

Chromatographie Liquide (LC) - Aspects Pratiques Ce système est conçu pour la chromatographie. Il doit être manipulé à l’aide de solvants appropriés et dans les limites de pressions, débits et températures décrites dans le guide d’utilisation. En cas d’utilisation en dehors des spécifications fournies par le fabricant, la protection assurée par l’équipement peut s’en trouver altérée.

Lors de la manipulation de solvants et d’échantillons potentiellement dangereux, il est important de suivre les procédures standards de sécurité au laboratoire. Les produits chimiques couramment utilisés en laboratoire peuvent être toxiques, corrosifs ou inflammables.

La liste exhaustive de tous les éléments relatifs à la sécurité sortirait du champ d’application de ce manuel, mais cependant, les points suivants doivent être pris en considération:

● Assurer une ventilation adéquate

● Port de lunettes de protection

● Port de gants lors de la manipulation des produits chimiques

● S’assurer que les récipients contenant des liquides sont sécurisés

● Interdiction de fumer. Aucune flamme nue à proximité

● Nettoyage en cas de fuite ou débordement

Ne jamais déconnecter une tubulure ou ouvrir une vanne sous pression. Arrêter d’abord la pompe et laisser revenir la pression à zéro. Porter des lunettes de sécurité. Si une tubulure se rompt ou si une vanne s’ouvre sous pression, un jet de liquide potentiellement dangereux peut être généré par la pompe.

En fonction de la toxicité des solvants utilisés, le système peut nécessiter une utilisation sous hotte de laboratoire.


Risque électrique

Risque électriqueSeul un représentant Gilson est habilité à retirer les panneaux de protection. S’il s’avérait nécessaire d’utiliser un cordon d’alimentation autre que celui d’origine, assurez-vous qu’il soit conforme aux spécifications suivantes ainsi qu’aux normes de sécurités locales en vigueur.

Modèle Union Européenne

● Connecteur A Mâle type E ou F “Schuko,”16 A

● Connecteur B Femelle IEC320/C13, 10 A

● 250 V~, H05VV-F 3G1.0mm²

Modèle Etats-Unis et Canada

● Connecteur A Mâle type NEMA 15-5, 15 A

● Connecteur B Femelle IEC320/C13, 10 A

● 125 V~, SVT 3x18AWG

Remplacer les fusibles grillés par des fusibles de taille et de calibre identiques à ceux décrits dans le manuel. Remplacer immédiatement tout cordon d’alimentation défectueux par un modèle équivalent. Assurez-vous que l’alimentation et la tension secteur correspondent bien à la valeur pour laquelle l’instrument est câblé.

Important

To ensure the safety of yourself and your coworkers, observe the following:

● Utiliser une prise de courant avec raccordement à la terre. La terre est nécessaire pour assurer la sécurité de l’opérateur ainsi que le fonctionnement correct de l’instrument.

● Sécuriser les solvants inflammables.

● La température des solvants à l’intérieur du système doit être inférieure de 25°C (77°F) à la température de flamme la plus basse des solvants utilisés.

● Lorsque l’instrument n’est pas utilisé, rincer les circuits hydrauliques à l’aide d’un solvant propre. Ne pas laisser de tampon dans le système, car il peut provoquer des blocages et endommager les joints.

● Avant de déplacer l’instrument, les connexions externes doivent être débranchées, les circuits hydrauliques rincés et des entrées ou des sorties bouchées.


General D

escription

General Description

PLC 2050/2250/2500 Purification Systems are designed for preparative liquid chromatography (PLC) to aspirate mobile phase solvents, form gradients, inject liquid samples, and provide solutions for a preparative column for high performance liquid chromatography (HPLC), centrifugal partition chromatography (CPC), or counter-current chromatography (CCC) applications. A detector measures the absorbance and sends the chromatogram to the Gilson Glider Prep Software, the onboard control software. Fractions are collected with the integrated fraction collector.

PLC 2050/2250/2500 Purification Systems Specifications

Flow Rate/Max Pressure

PLC 2050

● 50 mL/min, 300 bar (4 351 psi)

PLC 2250

● 250 mL/min, 230 bar (3 336 psi)

PLC 2500

● 500 mL/min, 110 bar (1 595 psi)

Flow Rate Performance

● 2% accuracy (with H²O degassed at 20°C)

● Repeatability better than 0.5%

Binary Gradient (Standard) or Quaternary (Optional) Injection

● Automatic or manual injection through sample loop with injection valve (optional)

● Additional injection pump (optional)

Gradient Former

● Linear response from 2 to 98 %

System Protection

● Software with minimum and maximum pressures adjustable

16 General Description PLC 2050/2250/2500 Purification Systems|

Gen

eral

Des

crip

tion

Control

● Via computer software “Gilson Glider Prep VX.X”

Storage

● Up to 32 methods in software memory and 80 GB of hard disk memory

AC Mains Supply Voltage

● 110V and 240 V~ ; 50/60 Hz (wattage: 350 W max.)

Electrical Protection

● Delayed action fuses T 6.3A H 250 V~

Power Distribution

● TT or TN power system only, transient over-voltages according to installation category II, Class 1 equipment, requires mains socket plug with protective earth terminal

Column Size with Optional Column Holder Kit

● Maximum height: 300 mm

● Maximum diameter: 115 mm

Detector Options

● Ultraviolet (UV) single wavelength detector, 254 nm filter

● UV dual wavelength detector, 254 and 280 nm filters (optional)

● UV detector 4-wavelength diode array detector (DAD) 200-400 nm (optional)

● UV/VIS detector 4-wavelength DAD 200-600 nm (or 200-840 nm) (optional)

Fraction Collector

● Several rack options available. See the Fraction Collector Parts section on page 71

● Backflush valve (automatic)

● Column switching valve (automatic or manual)

● Fraction collector cover


Contents

Detailed Description

Contents

Detailed Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

2- or 4-Way Low Pressure Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Dynamic Mixing Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Through-Loop Injection Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

UV-VIS Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Fraction Collector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Touchscreen Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

External Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Unpacking List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

18 Detailed Description PLC 2050/2250/2500 Purification Systems|

2-H

ead

Pum

p 2-Head PumpThe pump can deliver mobile phases at flow rates up to 500 mL/min (depending on the head size installed) with low residual pulsation. The PLC utilizes a high performance, dual piston, reciprocal pump. The pump generates suction through a system of pulleys, belts, and a stepper motor, which drives the cams and pistons. A manual valve at the outlet (black knurled knob) allows users to easily purge or prime the pump; the valve is associated with a pressure transducer, which monitors the pressure in the system.

Belt

Pulley

Pistons Motor

Cam CleaningDiscs

Figure 1: Pump Heads and Purge Valve

Figure 2: Interior View of Pump Head

Purge Valve

Pump Heads


2- or 4-Way Low

Pressure Valve

2- or 4-Way Low Pressure ValveA 2- or 4-way low pressure valve is located on the left side of the instrument, which permits binary or quaternary elution gradients.

Dynamic Mixing ChamberLocated at the outlet of the pump, the dynamic mixing chamber utilizes a motor-driven, magnetic agitator to thoroughly mix solvents and ensure smooth gradients at high flow rates.

Mixing volume depends on pump model installed:

● 4 mL for a 50 mL/min pump (PLC 2050)



The volume can be modified by replacing the chamber piston. See the Replace Mixing Chamber Piston section on page 53

Figure 3: 4-Way Inlet Valve

Figure 4: Dynamic Mixing Chamber


Thro

ugh-

Loop

Inje

ctio

n Va

lve Through-Loop Injection Valve

The 6-way through-loop injection valve allows users to load a sample in the loop while the system is running. The sample can be manually or automatically injected into the column by switching the valve.

UV-VIS DetectorThe ultraviolet-visible light (UV-VIS) detector measures the amount of light absorbed by a liquid passing through a flow cell at a given wavelength, called absorbance. It is equipped with a deuterium lamp, which uses interference filters to identify desired wavelengths or, with diode array detector (DAD) models, a range of wavelengths from 200 to 840 nm.

With UV-VIS and DAD models, the control software can acquire signals issued from four different wavelengths simultaneously. In addition, a continuous scan allows the acquisition of a full spectrum, which is displayed on the main screen.

Figure 5: Through-Loop Injection Valve

Figure 6: Through-Loop Injection Valve


Fraction Collector

Fraction CollectorAfter detection of the signal (chromatogram), the results obtained at the outlet of the column (peaks) can be directed to tubes on racks. A 3-way valve allows users to direct fractions to collection tubes or to waste. The integrated collector has three stainless steel racks designed for standard, 18 mm diameter tubes, accommodating up to 192 tubes and a maximum volume of 6.1 liters.

Figure 7: Collection Racks

Dispense Head Dispense Arm

Needle

Racks

Tubes


Touc

hscr

een

Com

pute

r Touchscreen ComputerThe system is controlled by a touchscreen computer, which allows the user to create methods and run the instrument. Four USB connectors are also available. Two are located in front of the system and below the screen. Two more are located on the sides of the instrument. USB cables and all other external connectors should not exceed 6.5 ft (2 m).

External ConnectionsAdditional external connectors, such as RS232, analog signal, and LAN inputs, can be used to connect devices, such as a second detector.

Stylus

Power Light

Touchscreen

USBPorts

Figure 8: Touchsccreen Computer

Figure 9: RS232 and LAN Inputs

Figure 10: USB and Analog Ports

USB Port

External Controls(5V = Max)

OptionalLevel Sensors(5V = Max)

Analog SignalInputs

(-1V = ; +1V =)

Serial Communications Ports LAN (Local Area Network) Plug


Unpacking List

Unpacking List

PLC 2050/2250/2500 Purification Systems (Standard)

1. Tubing with Fittings, Ethylene tetrafluoroethylene (ETFE), 1/8"(OD)

● Inlet, 1.5 m, 2.4 mm (ID) (x 2)

○ with a 20 cm spring at one end and with a stainless steel ballast at the other

● Waste Outlet, 1.5 m, 1.5 mm (ID)

○ with a stainless steel ballast at one end

● Prime/Purge Outlet, 1.5 m, 1.6 mm (ID)


● Waste Outlet for Loop Injection Valve, 1.5 m, 1.6 mm (ID)


2. Gradient Valve and Pump Head Tubing Assembly

● Tubes (x3)

● Springs

● Fittings

● Tee Union

3. Cleaning Disc Fittings

● Polyether ether ketone (PEEK) Nuts, 1/8" (x2)

● ETFE Ferrules, 1/8" (x2)

4. Loop Connection Fittings (Optional)

● Stainless Steel (SS) Nuts, 1/8" (x2)

● SS Ferrules, 1/8" (x2)


Unp

acki

ng L

ist 5. Preparative Column Tubing

● SS or PEEK Tubing, 1 m, 1/8" or 1/16" (OD), 2.1 or 1 mm (ID), 0.75 mm (ID) for Column Inlet

● ETFE Tubing, 1 m, 1/8" or 1/16" (OD), 1.6 or 1 mm (ID), 1 mm for Column Outlet

6. Preparative Column Fittings

● SS Nuts, 1/8" or 1/16" (x4)

● SS Ferrules, 1/8" or 1/16" (x4)

7. Column Holders

● 0–20 mm diameter

● 20–40 mm diameter

8. Detector Test Cell (for Service)

9. Rack Set

● SS Racks, 18.64 L for 18 mm diameter tubes

10. ETFE Luer Male-to-1/4"-28(TPI) Male (Possibly Used as a Collector Probe)

11. Positioning Guide for Placing Short Racks

12. Stainless Steel Trays for Bottles with Adhesive Feet

● Square Tray (x1)

● Rectangular Tray (x1)

13. USB Drive

● User’s Guide, Gilson Glider Prep (GGP) Software

● Gilson Glider Prep Installation File (.exe)

● Gilson Glider Prep Freeware Demo (.exe)

14. Stylus with Adhesive Holder

15. Power Cord (Model Based on Destination Country)


Unpacking List

16. Documentation

● PLC 2050/2250/2500 Purification Systems User’s Guide

● Gilson Glider Prep Software User’s Guide

● ECOM UV-VIS Diode Array Detector User’s Guide


Unp

acki

ng L

ist PLC 2050/2250/2500 Purification Systems

(Optional Accessories)

1. Quaternary Gradient System 1/8" (for 50 and 250 mL/min)

● Valve, 4 Inlets, 2 Outlets with Cable, Connector, and Holder

● Section Tubing with ETFE Fittings, 1.5 m, 1/8", 2.4 mm (ID), 20 cm Spring and Ballast (x4)

2. Quaternary Gradient System 3/16" (for 500 mL/min)

● Valve, 4 Inlets, 2 Outlets with Cable, Connector, and Holder

● Section Tubing with PTFE Fittings, 3/16

3. Loop Injection Valve (Automatic)

● 6-way, 2-position Electric Valve for 1/8"Tubing, Special Screws, and Cable

● Control Module, Power Supply, Bracket, and Cables

● Tubing Kit for 50 mL/min System (Option 1)

● Tubing Kit for 250–500 mL/min System (Option 2)

4. Loop Injection Valve (Manual)

● 6-way, 2-position Manual Valve for 1/8" Tubing and Bracket



● Hardware and Installation Procedure

5. Backflush Valve

● 4-way, 2-position Electric Valve for 1/8" Tubing, Special Screws, and Cable





Unpacking List

6. Column Switching Valve (Automatic)

● 6-way, 2-position Electric Valve for 1/8" Tubing, Special Screws, and Cable




7. Column Switching Valve (Manual)

● 6-way, 2-position Manual Valve for 1/8" Tubing and Bracket




8. Solvent Level Sensors, 3-3 for Binary System

● Communication Interface, 3-3 and Cable

● Sensors and Cables (x3)

● Straps, 80 cm (x3)

● Removable Cable Ties (x3)

● Power Adapter


9. Solvent Level Sensors, 5-5 for Quaternary System

● Communication Interface, 5-5 and Cable

● Sensors and Cables (x5)

● Straps, 80 cm (x5)

● Removable Cable Ties (x5)

● Power Adapter



Contents

Installation

Contents

Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Placing the Solvent Trays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Install Hydraulic Plumbing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Inlet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Ballasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Valve Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Waste . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Purge Valve Outlet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Injection Valve Waste Outlet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Injection Loop Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Position the Solvent Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Column Set Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Prepare the Fraction Collector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Position the Stylus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Connect the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

30 Installation PLC 2050/2250/2500 Purification Systems|

Plac

e th

e Sy

stem Place the System

After unpacking the system, place it at suitable location. Its total weight is approximately 143 lbs. (65 kg). Utilize two people whenever transporting the instrument, and grip it by the bottom plate. Free spaces of at least 20 cm must be kept around the system at all times to allow for proper tubing and cable connections and to provide adequate ventilation during operation.

Placing the Solvent TraysPlace the two stainless steel trays atop the instrument to store solvent and waste bottles. The trays cover the whole system and are designed to minimize leakage risks.

Air Vents

Figure 1: Air Vents

Figure 2: Solvent Trays

Rectangular Tray

Square Tray


Install Hydraulic Plum

bing

Install Hydraulic Plumbing The tubes situated outside the housing are delivered separately to avoid damage during transport, except the stainless steel tubings, which are already equipped with the fittings (nuts and ferrules) needed to connect them to the system.

Tubing for Low-Pressure Circuits

● ETFE, 1/8", 2.4 mm (ID) for Inlet of 50 and 250 mL/min Systems (PLC 2050 and PLC 2250)

● PTFE, 3/16", 1/8" (ID) for Inlet of 500 mL/min Systems (PLC 2500)

● ETFE, 1/16", 1 mm (ID) for 50 mL/min Systems (PLC 2050)

● ETFE, 1/8", 1.6 mm (ID) for 250 and 500 mL/min Systems (PLC 2250 and PLC 2500)

Tubing for High-Pressure Circuits

● SS, 1/8", 2.1 mm (ID) for 250 and 500 mL/min Systems (PLC 2250 and PLC 2500)

● SS, 1/16", 1 mm (ID) for 50 mL/min Systems (PLC 2050)

● Other Sizes Supplied According to User Requirements (PEEK)

Inlet Use the two (or four, depending on the model) inlet tubes supplied. Take care not to bend the tubes. For the A tube, take the end with the fitting and a long spring and put the ferrule in the PEEK body of the inlet valve marked A. Screw in the PEEK nut, finger-tight, and position it properly along the spring. Repeat the same operation for B (and also C and D for quaternary systems).

Figure 3: Inlet Tubes

Gradient Valve Body

Plug

Spring

ETFE Ferrule

PEEK Nut


Balla

sts Ballasts

To ensure pumping continuity for solvents and to prevent air from entering hydraulic circuits, tube ends are equipped with ballasts, which weight the tubes to ensure they stay at the bottom of containers.

Valve Connections1. Locate the three tubes with fittings and tee union labeled Gradient Valve to Pump Heads.

2. Screw two PEEK nuts into the check valve housing beneath the pump heads without fully tightening them.

3. Screw the PEEK nut into the Gradient Valve Outlet on the left side.

4. Finger-tighten the three fittings and ensure that the three fittings on the tee union are fully tightened.

Ballast

PEEK Nut

Figure 4: Ballasts

Tee UnionCheck Valve HousingGradient

Valve Outlet

Figure 5: Valve Connections


Waste

Waste1. Connect the Waste Tube directly to the Collection Valve located at the back of the system. It is

equipped with a ballast.

2. Take the flared end of the tube and place it in the free end of the valve.

3. Finger-tighten the white, polypropylene (PP) nut into the valve. If needed, gradually tighten the nut with an 8 mm spanner.

Purge Valve OutletConnect the tube to the Purge Valve Outlet and tighten the SS nut with a 3/8" spanner.

Figure 6: Waste Outlets

To Fraction Collector Needle

Collection Valve

Waste Outlet

Polypro Nut

Inlet

Purge Valve Outlet

Figure 7: Purge Valve Outlets


Inje

ctio

n Va

lve

Was

te O

utle

t Injection Valve Waste Outlet(Used only for Automatic Loop Injection Valve)

Connect the tube and hand-tighten the PEEK nut to the shut-off valve.

Injection Loop ConnectionConnect an Injection Loop to the 6-way Injection Valve:

● Between ports 3 and 6 for an Automatic Loop Injection Valve

● Between ports 1 and 4 for a Manual Loop Injection Valve

The loop must be equipped with SS nuts for 1/8" tubing (5/16-24 UNF model) and SS ferrules for 1/8" tubing (fittings supplied). Use a 3/8" spanner to tighten the nuts and properly crimp the ferrules.

Position the Solvent BottlesFinish the hydraulic installation by positioning the solvent and waste containers on the stainless steel trays atop PLC 2050/2250/2500 Purification Systems.

Waste Outlet

Shut-Off Valve

(Connected to Way n°2 of the Valve)

Figure 8: Injection Valve Waste Outlet

Figure 9: Injection Loop Connections


Column Set U

p

Column Set Up A wide range of columns can be used with PLC 2050/2250/2500 Purification Systems, including normal or reverse phase Columns and those with an overall external diameter of up to 40 mm. The device used to install the column is a tightening claw (black holder) located on the left side of the system. Two models are supplied, one for columns from 20 to 40 mm diameter and the other for columns up to 20 mm.

To install the column, loosen the Knurled Screw n°1, then slide the column inside the claw and strongly tighten the same screw. To replace the tightening claw, loosen the Knurled Screw n°2 and completely remove it. Replace it with the other holder, and then tighten the n°2 Knurled Screw.

Depending on the total weight and size of the column, it may be necessary to use the additional column holder kit, which increases the maximum external diameter to 115 mm and the maximum height to 300 mm.

Connect the inlet and outlet tubing to the column. Connect the Column Inlet to the Loop Injection Valve Outlet (Automatic: way n°5, Manual: way n°3). Connect the Column Outlet to the bulkhead union (Detector Inlet).

If additional valves are installed, such as the Backflush Valve or Column Switching Valve, proceed as indicated on the system. Use a 3/8" spanner to tighten the nuts and properly crimp the ferrules.

n°2

n°1

Figure 10: Column Positioning


Prep

are

the

Frac

tion

Col

lect

or Prepare the Fraction CollectorInstall racks appropriate to the type of collection needed. The standard rack supplied is Rack Set, 18x150 - 180 mm Tubes, PLC A, composed of three racks with 192 tubes, 180 mm high with a total collection volume of 6.1 L.

NOTICE Remove the black cable tie which holds the dispense arm during transfer.

Place the tubes in the rack holes, and then place the racks on the tray. Notches in the racks fit into positioning guides mounted on the system.

Black Cable TieDispense Arm

Figure 11: Black Cable Tie

Figure 12: Short Rack Guide

Back Guides

Positioning NotchFront Guides


Prepare the Fraction Collector

When using short racks, use the additional positioning guide supplied. An optional tray platform is also available. It is designed for elevating racks with small tube heights.

Two different models of probe can be connected to the dispense head of the fraction collector. The installed needle is SS, 40 mm. An accessory probe is available, which is shorter, but with a smaller outlet diameter for a fine and accurate dispense. A 70 mm probe is available to collect fractions from 150 mm high tubes. In this case, the tray platform cannot be used.

Figure 13: Short Rack Guide

Figure 14: Tray Platform

Positioning Guide for Short Racks

Additional Probe (ETFE Luer Male-to-1/4"-28-Male

Figure 15: Probes

Standard Probe


Posi

tion

the

Styl

us Position the StylusUse the stylus to control the integrated software on the touchscreen computer. A fixed holder provides a space for the stylus and cord. The stylus holder can be placed anywhere the user desires. In the picture, the stylus is attached to the right side of the instrument.

Connect the SystemThe complete system is supplied with appropriate fuses and is ready to operate at the voltage of the destination country. If needed, a selector located near the power plug allows users to adjust the voltage of the instrument.

Plug in the AC cord into the main power switch located on the right side of the instrument.

Fuses

● 110V and 240 V, 50/60 Hz, 350 W Max

● General Fuses: T 6.3A H 250 V (x2)

Figure 16: Stylus and Holder

Power CordON/OFF Switch

Fuse Drawer

Figure 17: Power Cord and Switch


Manual Loop Injection Valve

Options

Manual Loop Injection Valve

Column Switching Valve (Manual)

NOTICE Never switch the manual valve during the run or irreversible damage will occur.

COLU

MN LOAD

P

W

W

3 4

5 1

2

6

COLU

MN INJ

P

W

W

3 4

5 1

2

6

Load Inject

Figure 18: Loop Injection Valve with Inject and Load Diagrams

5 4

3

1

6 2

COLU

MN

2

COLU

MN

1

OUT

IN

From Inj.

To UV

5 4

3

1

6 2

COLU

MN

2

COLU

MN

1

OUT

IN

From Inj.

To UV

Figure 19: Column Switching Valve with Flow Diagrams

40 Options PLC 2050/2250/2500 Purification Systems|

Acq

uisi

tion

Mod

ule

for D

etec

tors Acquisition Module for Detectors

Column Holder Kit for Heavier HPLC Columns ● Up to 50 mm (OD)

Figure 20: Acquisition Module

Figure 21: Heavy Column Holder


Bar Code Reader

Bar Code Reader

Fraction Collector Cover

Figure 22: Bar Code Reader

Collector Cover with Magnetic Lock

Adaptor for Pipe Connection and Air Extraction

Transparent Door

Figure 23: Fraction Collector Cover

42 Options PLC 2050/2250/2500 Purification Systems|

Rack

s Racks

Rack Overlays, 18 mm Tubes, PLC A and Rack Overlays, 13 mm Tubes, PLC A with Overlays for Tube Identification

Solvent Level Sensor

Figure 24: Tube Racks

Figure 25: Sensor Placement

Figure 26: Sensor Outputs


Solvent Level Installation

Solvent Level Installation

Figure 27: Level Sensor Installation Steps

2 3

4

1

5 6


Contents

Operation

Contents

Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Prime the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Automatic Loop Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Manual Loop Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

46 Operation PLC 2050/2250/2500 Purification Systems|

Cont

rol t

he S

yste

m Control the SystemWhen all the installations and connections are made, switch on the system. The components of the instrument will initialize and the Gilson Glider Prep (GGP) Software will automatically start.

Once the software loads, several graphics will appear, displaying in real time the status of the instrument and related components. The application was developed to be intuitive and easy to use. Refer to the GGP User’s Guide for more explanation.

Use the stylus to control the touchscreen and operate the system.

Figure 28: Gilson Glider Prep Control Screen


Prime the Pum

p

Prime the PumpWhen tubing is empty and the pump is unprimed, it may be difficult to pump new solvent into the system. To ease this process, launch the Purge mode in GGP, and then lightly unscrew the Knurled Knob Purge Valve located above the pumps. Once solvent has flowed through the ETFE Outlet Tube, stop the pump and close the Purge Valve.

Figure 29: Knurled Knob

Figure 30: Close Purge Valve Message

Knurled Knob

48 Operation PLC 2050/2250/2500 Purification Systems|

Aut

omat

ic L

oop

Inje

ctio

n Automatic Loop InjectionTo automatically inject a sample of 1–50 mL, use the Loop Injection 6-way Valve mounted on the system.

The loop should be loaded when the system is stopped or during an Equilibration Phase. In both cases, the red LED Load light will be activated.

1. Fill a syringe with the desired volume of sample and place it in the Luer Female Fitting in the top position of the Injection Valve (way n°1).

2. Inject the sample into the loop. Any surplus will flow to the Waste Tube (way n°2).

When the starting mode Valve Injection is selected in the method edition, the injection of the sample is automatically done before the starting of the elution phase (green LED Inj. lighted). The loop gets in the hydraulic circuit of the pump, the sample is pushed in the chromatography column by the solvents pumped.

Figure 31: Automatic Load and Injection Diagram

COLU

MN

1 2

3 5

6

4

LOAD

W

P

COLU

MN

1 2

3 5

6

4

INJ

W

P


Manual Loop Injection

Manual Loop InjectionTo manually inject a sample of 1–50 mL, use the Loop Injection 6-Way Valve mounted on the system.

The loop should be loaded when the system is stopped or during an Equilibration Phase.

1. Fill a syringe with the desired volume of sample and place it in the Luer Female Fitting in front of the Injection Valve (probe port).

2. Inject the sample into the loop. Any surplus will flow to the Waste Tube (way n°6).

When the starting mode Wait for Injection is selected in the method edition, the starting of the Elution Phase will wait for the manual switching of the valve and the confirmation by the user.

The sample is pumped into the hydraulic circuit and is pushed into the chromatography column via the solvents used.

COLU

MN LOAD

P

W

W

3 4

5 1

2

6 CO

LUM

N INJ

P

W

W

3 4

5 1

2

6

Figure 32: Manual Load and Inject Diagram

Figure 33: Wait for Injection Prompt


Contents

Maintenance

Contents

Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

Clean the Pistons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Replace Mixing Chamber Piston . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Pump Head Disassembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Replace a Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Replace the Check Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Replace the Detector Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Maintenance Schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

52 Maintenance PLC 2050/2250/2500 Purification Systems|

Intr

oduc

tion Introduction

This chapter describes actions that should be performed on a routine basis to ensure long term safe and trouble free operation of the system. The frequency of the maintenance activities is dependent on the nature of the application, such as the solvents used, the volume of the mobile phases delivered by the pump, level of cleanliness of the facility, etc. The pump has been designed for reliability and needs very little routine maintenance when operated correctly.

Reduced accuracy of the pump may be caused by seal wear or to a fault in the check valves. This section provides information and procedures about how to replace consumable parts and how to maintain the instrument.

Clean the PistonsThe high pressure heads incorporate a piston washing device called Cleaning Discs. These are typically used when aqueous buffers are pumped and function to manually wash the buffers from the backs of the seals and to prevent early wear of the seals.

Fit the 1/8" PEEK nuts and ETFE ferrules onto suitable lengths of wide bore 1/8" PTFE tubing. Screw the fittings into position in the Cleaning Discs Inlet and Outlet at the back of the high pressure heads, one for the inlet, and the other for the outlet. The outlet PTFE tubing goes to waste and the process adapted to the inlet is chosen by the user, an association Luer + Syringe or another pumping system.

Cleaning Discs

Figure 34: Piston Cleaning Discs

Inlet and Outlet for Cleaning Discs

Junction Tube for Cleaning Discs


Replace Mixing Cham

ber Piston

Replace Mixing Chamber PistonThe mixing chamber volume depends on the model of the pump :




These volumes are calculated to give the best performances among the specified flow rate range, but they can be easily modified by replacing the Mixing Chamber Piston.

For example, for a 500 mL/min system (supplied with 20 mL chamber) at 50 mL/min, it can be useful to replace the Chamber Piston to have better mixing.

1. Purge the system with a suitable solvent, switch it off, and then disconnect it from the mains.

2. Disconnect the stainless steel tube from the chamber outlet with a 3/8" spanner with SS nut. You have to entirely remove the tube, i.e., disconnect it also from the injection valve inlet.

3. Remove the four screws (Allen wrench, 4 mm) and carefully pull the piston to remove it.

2. Get a new piston (with its PTFE seal already inserted) to ensure you will install it in the right way (with the threaded hole on top) and slide it gently into the chamber.

3. Replace the four fixing screws and tighten them with a church key.

4. Replace the tubing and tighten the SS nuts with a spanner.

Figure 35: Replacing the Mixing Chamber Piston

PTFE Seal for Mixing Chamber Piston

Stainless Steel Tube

Pump Head Screws Stainless Steel Tube


Pum

p H

ead

Dis

asse

mbl

y Pump Head DisassemblyWhen disassembling or reassembling the pump, make sure that each component is clean and take care that the system is assembled in a clean environment.

Remove the pump head if you need to replace the seal:

1. Purge the pump head with a suitable solvent.

2. Switch off the system and disconnect it from the mains.

3. Disconnect the ETFE inlet tube by hand (PEEK nut).

4. Disconnect the stainless steel outlet tube with a 3/8" spanner (SS nut). You have to remove the tube (i.e., disconnect it from the purge valve holder).

5. Remove the four screws with a 3 mm Allen wrench. Slowly unscrew one screw, and then the one diagonally opposite.

6. Carefully remove the pump head. The pistons and cleaning discs (PEEK) will be visible.

Figure 36: Pump Head and Cleaning Disc Removal

Stainless Steel Tubes

Pump Head Screws


Pump H

ead Disassem

bly7. Carefully remove the cleaning discs to fully expose the pistons.

Pistons

Cleaning Discs

Pistons

Figure 37: Exposed Cleaning Discs, 50 mL/min Pump

Figure 38: Exposed Pistons, 250 mL/min Pump


Repl

ace

a Se

al Replace a SealThere are four seals in all: one for every Pump Head and one on every Cleaning Disc.

1. Once the part is removed, carefully take out the old seal to avoid scoring the seat of the seal. If the seat is scored, it is necessary to replace the Pump Head or the Cleaning Disc, which is not under warranty.

2. Thoroughly clean the Seal Seat with alcohol to remove possible worn seal particles and plunge the new Seal in Propanol-2 for lubrication.

3. Correctly position the new Seal on the Pump Head or Cleaning Disc.

4. Use the special tools, A and B, pictured below.

Figure 39: Seal Replacement

Back Head

Back Cleaning Discks

Seals

Piston Seal


Replace a Seal1. Position the part A and slip the part B into A.

2. Gently hit part B with your fist.

3. Remove the tool and note that the seal is properly placed.

4. Put back the Cleaning Disc on the Piston and insert it inside the Heads Holder.

5. See that the alignment between the Cleaning Disc and the Heads Holder is correct.

6. Put back the Pump Head on the Piston and replace the 4 fixing screws slowly with care, one screw at a time and then the one diagonally opposite.

7. Reconnect the tubing.

NOTICE The life of the seals is dependent on the flow rate, pressure, type of liquids used and temperature, but mostly on the cleanliness of the mobile phase and sample. The presence of micro-particles will cause accelerated wear and tear of the seals. Similarly, any dried buffer particles on the piston will damage the seal.

Figure 40: Seal Seating Instructions

A

B


Repl

ace

the

Chec

k Va

lve Replace the Check Valve

Abnormal pressure fluctuation or the observation of pump noise on the data system is usually caused by check valve problems. The noise is regular in nature and is caused by pressure and flow variation. Pump accuracy is also compromised. Details on check valve repairs are given below.

There are four check valves, two per head, one for each inlet and one for each outlet (model 10 mm). If they become dirty, they will not open and close correctly for a long time.

1. Purge the pump head with a suitable solvent.

2. Switch off the system and disconnect it from the mains.

3. Disconnect the ETFE inlet tube (PEEK nut).

4. Disconnect the Stainless Steel Outlet Tube with a 3/8" spanner (SS nut). You have to remove the tube (i.e., disconnect it also from the Purge Valve Holder).

5. Unscrew the Check Valve Housing using a 17 mm spanner.

Figure 41: Check Valve

Figure 42: Check Valve Inlets and Outlets

Inlet Check Valve Housing (Suction)

Outlet Check Valve Housing (Delivery)


Replace the Check Valve6. Clean the Check Valves by placing them in an ultrasonic bath with methanol and acetone for

approximately five minutes, or replace them with new ones.

7. Ensure that the arrow on the Check Valve is directed downwards or damage to the surface quality, the ball and the seat will occur.

8. Replace the Check Valves. Whether it is for the inlet or the outlet, make sure the arrow is always directed upwards.

9. Finger-tighten the Valve Housing in the Pump Head until you hear a little crunch, which means that the new check valve is properly seated. This noise corresponds to the collapsing of the top and bottom membranes.

10. Reconnect the tubing.

Check Valve (10 mm)

Check Valve (10 mm)

Check Valve Housing

Inlet(Suction)

Outlet(Delivery)

Figure 43: Check Valve Inlet and Outlet Placement


Repl

ace

the

Det

ecto

r Lam

p Replace the Detector LampIf efficiency of detection is unreliable, detector lamp wear could be to blame. Lamp life can be checked beforehand with the GGP Software via the menu: ? > Information.

System Preparation1. Purge the system with a suitable solvent and then with air.

2. Switch off the system and disconnect the power cord. It will be reconnected only when the panel will be installed back in its place.

3. Let the lamp cool down fifteen minutes after switching it off.

4. Take off the plastic panel on the left side by removing the 6 Domed-Head Screws with a 2.5 mm Allen Wrench.

Lamp Removal

1. Loosen the screw on the Lamp Socket behind the cell compartment using a T-20 Torx Adapter.

Figure 44: Deuterium Lamp


Replace the Detector Lam

p2. Turn the Lamp Socket to the diagonal position.

3. Take the Lamp Power Supply Cables and disconnect the 3-Pin White Connector.

4. Loosen and remove the two screws holding the lamp in place with a T-10 Torx Adapter.

5. Remove the lamp from the socket and get a new one.

NOTICE Never touch quartz bulb of the lamp with bare fingers to avoid damages causing faulty working of the detector.

6. Carefully insert the new lamp assuring that it is correctly positioned.

NOTICE Should you touch the glass of the lamp accidentally, clean it thoroughly with a lint free cloth and alcohol.

7. Screw it in securely and connect the plug in the 3-pole socket.

8. Reassemble the unit by performing the same steps in reverse order.

9. After the lamp replacement, recalibration and zeroing of the counter of operating hours must be carried out.


Replace the Detector Lam

p

Clean the Flow CellA contaminated cell in lowered light transmission may increase noise level and make difficulties at zeroing. The simplest cleaning method is to flush the dismantled cell with suitable solvents. The cell must be taken out of the instrument before washing.

1. Follow Steps 1 through 5 in the Lamp Removal section on page 60.

2. Remove the Flow Cell by tilting back the locking lever.

3. Disconnect the outlet and inlet tubing from the cell (polypropylene nuts).

4. Select the solvent type according to character of contamination by a series of mutually miscible solvents. It is possible to use both organic and inorganic solvents and diluted solutions of acids.

5. Flush the cell with the chosen solution and rinse extensively.

6. Reassemble the units.

NOTICE After the operation, check cell cleanliness using the test described in the Test of the Cell and Mobile Phase chapter in the Detector Manual supplied.

Figure 45: Flow Cell

Locking Lever


Mai

nten

ance

Sch

edul

e Maintenance ScheduleOperation Frequency

Verify the cleanliness of all liquid containers. Daily

Clean and rinse the gradient valve and all other switching valves with suitable solution. Perform maintenance any time an aqueous solution with strong buffers is used.

As Needed

Clean all parts in contact with solvents or samples with suitable solutions. Rinse thoroughly using a mix of water and alcohol. Perform maintenance any time an aqueous solution with strong buffers is used.

As Needed

Check the suitable tightening of fittings and the proper state of tubing (not bended). Perform maintenance any time solvent leaks or air bubbles are observed.

Weekly

Replace the tubing and associated fittings (except stainless steel tubing). Replace tubing and fittings any time damage is observed.

Yearly

Replace the seals. Yearly

Clean the check valves. Quarterly

Replace the check valves. Perform maintenance any time abnormal pressure, flow rate fluctuation, or noisy baselines occur.

Yearly

Clean the piston rods. Perform maintenance any time the pump head is disassembled.

Quarterly

Check the efficiency of the deuterium lamp (refer to the manual supplied). Perform maintenance any time higher noise levels or decreased sensitivity is observed.

Quarterly

Replace the deuterium lamp. When Efficiency is Unreliable

Clean the flow cell. Quarterly

Check fan operation Monthly


General

Warranty

GeneralPLC 2050/2250/2500 Purification Systems system is under warranty to be free from defects in material or workmanship under normal use within the instrument specifications indicated in this manual and under conditions given below.

The warranty period is one year from the date of initial shipment of the manufacturer. Note that it does not apply to the instrument if modified by the user or resold without permission from the manufacturer, nor to consumable parts after limited warranty expiration, nor to any failure of lifetime-expired parts.

The warranty is void in the following cases:

● Failure due to incorrect installation

● Failure due to using the incorrect AC power supply

● Failure due to mechanical force applied to the unit

● Failure due to improper handling by the user or an individual not authorized to operate the equipment

● Failure due to the use of improper spare parts or hardware

● Damage to the software, data, or hard disk due to a computer virus infection

● Corrosion of cabinet caused by leakage of solvent or samples

● Corrosion of electronic parts caused by highly corrosive atmospheric gas

● Failure due to the disassembly, modification, relocation, or transport after initial installation

● Failure due to the disconnection of main power without taking the specified normal shutdown procedure

● Failure due to the disregard of safety regulations

● Failure due to not following the upkeep schedule

● Failure due to acts of nature

● The manufacturer assumes no liability for any result caused to malfunction of PLC 2050/2250/2500 Purification Systems.

● Limited Warranty of Consumable Parts

66 Warranty PLC 2050/2250/2500 Purification Systems|

Lim

ited

War

rant

y Limited Warranty

Fittings, Unions, and TubingSee the Fittings Parts section on page 69 for detailed descriptions and part numbers.

Terms of Use:

● Normal tightening of fitting

● Use of clean solvents and containers

● Proper positioning, according to the instructions in this manual

Damages Not Covered:

● Damaged threads caused by improper tightening

● Blocked flow paths caused by improper installation or unsuitable mobile phases

ValvesSee the Valves Parts section on page 73 for detailed descriptions and part numbers.

Terms of Use:

● Normal tightening of fitting


● Following the preventative maintenance schedule


● Damaged threads caused by improper tightening



Limited W

arranty

PumpSee the Pump Parts section on page 72 for detailed descriptions and part numbers.

Terms of Use:




● Damaged parts caused by improper installation, replacement, or the use of unsuitable solvents


DetectorSee the Detector Parts section on page 71 for detailed descriptions and part numbers.

Terms of Use:




● Damaged parts caused by improper installation, replacement, or the use of unsuitable solvents



PLC Systems

Parts

PLC SystemsDesignation Part NumberPLC 2050 Purification System 21140001PLC 2250 Purification System 21140002PLC 2500 Purification System 21140003

FittingsDesignation Part NumberNUT, SSI GLAND, FOR 1/16" TBG 49031401FERRULE, SSI, FOR 1/16" TBG 49031402FERRULE, ETFE, NATURAL, FOR 1/8" TBG 49041015NNUT, FLANGELESS MALE, 1/8", BLK (P-301) 49041017FERRULE, FLANGELESS, 1/8", TEFZEL (P-300) 49041015SCREW NUT, FOR 1/8" TBG, SS 4903141043FERRULE, FOR 1/8" TBG, SS 4903141041NUT, FLANGELESS, PEEK FOR 3/16" TBG (P-132) 49040132FERRULE, ETFE, NAT, FOR 3/16" TBG (P-133N) 490410133NPLUG, ¼"-28, ETFE (P-311) 49041019PLUG, 5/16"-24, DELRIN (P-557) 490410557

70 Parts PLC 2050/2250/2500 Purification Systems|

Tubi

ng TubingDesignation Part NumberSuction Tube (ETFE - 1m50 - 1/8" - ID=2,4 mm) + PEEK nut + ETFE ferrule + spring (20 cm)

21040101

Suction Tube 500 (PTFE - 1m50 - 3/16" - ID=1/8") + PEEK nut + ETFE ferrule 21040102Waste Outlet Tube (ETFE - 1m50 - 1/8" - ID=1,6 mm) + Polypropylene nut (flared end) 21040115Waste Outlet Tube 500 (ETFE - 1m50 - 1/8" - ID=2,4 mm) + Polypropylene nut (flared-end)

21040116

ETFE Tubing OD=1/8" ID=2,4 mm (1 meter) 21040137ETFE Tubing OD=1/8" ID=1,6 mm (1 meter) 21040138PTFE Tubing OD=3/16" ID=1/8" (1 meter) for 500 systems 21040139Spring 20 cm for ETFE 1/8" Tubing 21040142SS 1/8" ID=2,1mm Preformed Tube "Right Head" to "Transducer Support" + Fittings 21040117SS 1/8" ID=2,1mm Preformed Tube "Left Head" to "Transducer Support" + Fittings 21040118SS 1/8" ID=2,1mm Preformed Tube "Transducer Support" to "Mixing Chamber" + Fittings

21040119

SS 1/8" ID=2,1mm Preformed Tube "Mixing Chamber" to "Injection Valve" + Fittings 21040120SS 1/8" ID=1mm Preformed Tube 50 "Right Head" to "Transducer Support" + Fittings 21040121SS 1/8" ID=1mm Preformed Tube 50 "Left Head" to "Transducer Support" + Fittings 21040122SS 1/8" ID=1mm Preformed Tube 50 "Transducer Support" to "Mixing Chamber" + Fittings

21040123

SS 1/8" ID=1mm Preformed Tube 50 "Mixing Chamber" to "Injection Valve" 21040124SS Tubing OD = 1/8" ID=2,1 mm (1 meter) for 250/500 Systems 21040140SS Tubing OD = 1/8" ID=1 mm (1 meter) for 50 Systems 21040141Sample Loop, 1 mL PLC A, 1/8" 21040030Sample Loop, 2 mL PLC A, 1/8" 21040031Sample Loop, 5 mL PLC A, 1/8" 21040032Sample Loop,10 mL PLC A, 1/8" 21040033Sample Loop, 20 mL PLC A, 1/8" 21040034Sample Loop, 30 mL PLC A, 1/8" 21040035Sample Loop, 40 mL PLC A, 1/8" 21040036Sample Loop, 50 mL PLC A, 1/8" 21040037


Fraction Collector

Fraction CollectorDesignation Part NumberRack Set, 18x150 - 180 mm Tubes, PLC A 21040019Rack Set, 13x100 mm Tubes, PLC A 21040020Rack Set, 16x150 mm Tubes, PLC A 21040021Rack Set, 21x150 mm Tubes, PLC A 21040022Rack Set, 25x150 mm Tubes, PLC A 21040023Rack Set, 28x150 mm Tubes, PLC A 21040024Rack Set, 29.5x150 mm Tubes, PLC A 21040025Rack Set, 13x73 mm Hemo Tubes, PLC A 21040026Rack Funnel, 16 Outlets, PLC A 21040027Rack Overlays, 18 mm Tubes, PLC A 21040028Rack Overlays, 13 mm Tubes, PLC A 21040029

GeneralDesignation Part NumberColumn Holder Kit, Prep Columns 21040015Upgrade Kit, PLC A50 TO 250 21040016Upgrade Kit, PLC A250 to 50 21040017Central Processing Unit with touchscreen 21040136Power Supply 24V DC 300W 21040134Power Supply 5V DC 15W 21040135

DetectorDesignation Part NumberDeuterium Lamp 21040113UV Detector, Dual Filter, 254/280 nm, PLCA 21040009Diode Array Detector, 4-Wavelengths, 200-400 NM, PLCA 21040010Diode Array Detector, 4-Wavelengths, 200-600 NM, PLCA 21040011Diode Array Detector, 4-Wavelengths, 200-800 NM, PLCA 21040012Acquisition Module for External Detectors 21040018Prep Flow Cell, 2.4 mm 21040125Flow Cell, PLC A, 1.3 mm 21040126Prep Flow Cell, PLC A, 0.3 mm 21040127

72 Parts PLC 2050/2250/2500 Purification Systems|

Pum

p PumpDesignation Part NumberCheck Valve 10 mm 21040103Quaternary Gradient, 1/8" (PLC A50 OR 250) 21040001Quaternary Gradient, 3/16“ (PLC A500) 21040002Seal 4.5 mm for 50 systems 21040104Seal 10 mm for 250 systems 21040105Seal 14 mm for 500 systems 21040106Cleaning disc 4.5 mm for 50 systems 21040110Cleaning disc 10 mm for 250 systems 21040111Cleaning disc 14 mm for 500 systems 21040112Piston 4.5x65 + kit for 50 systems 21040107Piston 10x65 + kit for 250 systems 21040108

Piston 14x65 mm, PLC A500 21040109

PTFE seal 18.72 for mixing chamber piston 21040114

ElectronicsDesignation Part NumberElectronic board CPU2008-RP (blue = pump) 21040129Electronic board CF2008-R (red = collector) 21040130Electronic board CM2008 (mother board) 21040128Electronic board CIP2008 (pump interface) 21040131Electronic board CIC2008 (external connections interface) 21040132Electronic board + "Flex" for collector connections 21040133Electronic board CPU2008-RP (blue = pump) 21040129Electronic board CF2008-R (red = collector) 21040130Electronic board CM2008 (mother board) 21040128Electronic board CIP2008 (pump interface) 21040131Electronic board CIC2008 (external connections interface) 21040132

Electronic board + "Flex" for collector connections 21040133


Valves

ValvesDesignation Part NumberAutomatic Injection Valve with Tubing Kit PLC A50 21040003Automatic Injection Valve with Tubing Kit PLC A250/500 21040004Automatic Backflush Valve with Tubing Kit PLC A50 21040005Automatic Backflush Valve with Tubing Kit PLC A250/500 21040006Column Selection Valve with Tubing Kit PLC A50 21040007Column Selection Valve with Tubing Kit PLC A250/500 21040008Manual Column Valve with Tubing Kit PLC A50 21040013Manual Column Valve with Tubing Kit PLC A250/500 21040014

Documents

PLC 2050/2250/2500 Purification Systems - Gilson Guide_Gilson PLC 2050, 2250 and 2… · 2 Tae o Contents | PLC 2050/2250/2500 Purification Systems Table of Contents Table of Contents1