Supporting Material

Polyacrylamide-Based Biocompatible Nanoplatform Enhances the Tumor Uptake,

PET/fluorescence Imaging and Anticancer Activity of a Chlorophyll-a Analog

Anurag Gupta1, Shouyan Wang2, Aimee Marko1, Penny Joshi1, Manivannan Ethirajan,

Yihui Chen1, Rutao Rao3, Munawwar Sajjad3, Raoul Kopelman2 and Ravindra K. Pandey1,*

1PDT Center, Cell Stress Biology,, Roswell Park Cancer Institute, Buffalo, NY 14263,

2Department of Chemistry, University of Michigan, Ann Arbor, MI 48109

3Department of Nuclear Medicine, State University of New York, Buffalo, NY 14221

_____________________________

To whom correspondence should be addressed:

Ravindra K. Pandey (ravindra.pandey@roswellpark.org)

Contents:

1. Figure S1: Characterization of the size of the PAA nanoparticle formulation used for

Photodynamic Therapy/fluorescence reflectance imaging and toxicology studies

2. Figure S2: Biodistribution of PS2: 24, 48, and 72 H post-injection in 10% ethanol without NPs.

3. Figure S3: SEM images of lypholyzed PAA-based NPs.

4. Figure S4: Zeta potential of free PAA NPs

5. Figure S5: Zeta potential of PS1 post-loaded in PAA NPS

6. Figure S6: OSEM2D rendered digital PET imaging movie of PS2, NP2, NP4 and NP5 at 48h

post-injection (BALB/c mice bearing Colon 26 tumors).

Figure S1: The DLS for (A) Blank PAA NPs used for the toxicological studies, (B) NP1 in Tween-80 / PBS (concentration of Tween-80 is < 1% for (A) and (B)) and (C) NP3, Cyanine Dye Conjugated to AFPAA NPs. The mean diameter is 30 nm, and 35.1 nm, and 53.8 nm for A, B, and C, respectively.

B A

C

Figure S2: In vivo biodistribution of PS2 in 10% ethanol (without NPs) at 24, 48, and 72 hours post tail vein injection in BALB/c (3 mice/group) mice bearing subcutaneous Colon26 tumors on the right shoulders.

Figure S3. SEM images of lypholyzed PAA-based NPs in powder form, whereas the other SEM images presented in the main text (Fig. 5) are NPs suspended in liquid and then dried prior to being imaged.

Figure S4. Zeta potential of free PAA-NP

Figure S5. Zeta potential of PS1 post-loaded in PAA NPs.

PS2_OSEM2D rotation - 48 h.mpg NP2_1p4mg_OSEM2D rotation - 48 h.mpg

NP4_OSEM 2D rotation - 48 h.mpg NP5_OSEM 2D rotation - 48 h.mpg

Figure S6: OSEM2D rendered digital PET imaging movie of PS2, NP2, NP4 and NP5 at 48h post-

injection (BALB/c mice bearing Colon 26 tumors).