Polycystin-1 Antibodies (E1, 2 and 3, 4) Support: 1-410-706-5804 [email protected] Web: http://www.baltimorepkdcenter.org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`@*' K#)/O#&H <)/N?*% <'K $%*&# <% 534_I *& 5a4_IC UO*/K &#@#<%#K L&##b# T %J<c AHA)#$C =7(5&:- 03B-5< GdSW @^eC3 <'K 64f ,)HA#&*) >()%-)75&'()< 044 D) <% 344 D,T>) C35$71< G&*%#/' R @?&/g#K ./, 02 =>>?'*()*% K#%#AB*' *L &#A*>(/'<'% J?><' *& >*?$# GI0 (H \058 *& e\03C IP IB: 7E12 E1 E2 E3 E4 7E12 Short exposure Long exposure IB:CC ./, 32 =>>?'*@&#A/@/%<%</*' *L #'K*,#'*?$ @*)HAH$B'50 L&*> >*?$# )?', B$$?# )H$<%#$ (H \058 *& e\03C =>>?'*@&#/@/%<%#K GI0 /$ K#%#A%#K (H />>?'*()*% (H e\03 *& <'B5II -K/&#A%#K <,</'$% XJ# I5%#&>/'<) %</)1 5 !m AcTubulin E3 AcTubulin/E3 AcTubulin/E3/DAPI Collecting duct cells ./, F2 =>>?'*E?*&#$A#'A# K#%#AB*' *L #'K*,#'*?$ @*)HAH$B'50 /' %J# A/)/< *L >*?$# A*))#AB', K?A% A#))$ (H \FC 225- 150- E1, 2 E3, 4 105- kDa: 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 E1 E2 E3 E4 7E12 1: HEK-pcDNA5 2: HEK-human PKD1 3: HEK-mouse PKD1 LRR C-Lectin PKD REJ GPS NTF CTF 7E12 anti-CC H L T3041 PC1

Polycystin-1 Antibodies (E1, 2 and ... - Baltimore PKD Centerbaltimorepkdcenter.org/downloads/Figure 1 (Datasheet).pdf · Polycystin-1 Antibodies (E1, 2 and 3, 4) Support: 1-410-706-5804

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Page 1: Polycystin-1 Antibodies (E1, 2 and ... - Baltimore PKD Centerbaltimorepkdcenter.org/downloads/Figure 1 (Datasheet).pdf · Polycystin-1 Antibodies (E1, 2 and 3, 4) Support: 1-410-706-5804

Polycystin-1 Antibodies (E1, 2 and 3, 4) Support: 1-410-706-5804

[email protected] Web: http://www.baltimorepkdcenter.org/

!""#$%&'()*+ + + +,-%(..-)/-/+!)'0(/1+2$#3'()*++ +++++++++++++4(#-%3#&5+67 +++!!!!!"#$%#&'!()*+',!!-./,!01 ! !02344502644 !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!7894!:;<!!!!!!!!!!!=>>?'*@&#A/@/%<B*'!-./,!31 ! !4C053!D,!@#&!3!>,!%*%<)!@&*%#/'$!!!!!!=>>?'*E?*&#$A#'A#!-./,!F1! ! !0264!

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

!!;..3)(:-)*<!Z[[!K*></'!*L!J?><'!GI02!\0!<'K!\3]!

!!!!!!!!!!!I5)#AB'!K*></'!*L!J?><'!GI02!\F!<'K!\8!!="-%$-*+>5(**?,-&%'@$71+<+^?><'!<'K!>*?$#!-%#$%#K1!A(5.<+Z/N?/K!=7(5&:-+$)*753%'()*+SJ/@@#K!<%!8_IC!`@*'!K#)/O#&H!<)/N?*%!<'K!$%*&#!<%!534_I!*&!5a4_IC!UO*/K!&#@#<%#K!L&##b#!T!%J<c!AHA)#$C!=7(5&:-+03B-5<+GdSW!@^eC3!<'K!64f!,)HA#&*)!>()%-)75&'()<+044!D)!<%!344!D,T>)!!C35$71<+G&*%#/'!R!@?&/g#K!!!

./,!02!=>>?'*()*%!K#%#AB*'!*L!&#A*>(/'<'%!J?><'!*&!>*?$#!GI0!(H!\058!*&!e\03C!!

IB: 7E12

E1 E2 E3 E4 7E12

Short exposure

Long exposure

IB:CC

./,! 32! =>>?'*@&#A/@/%<%</*'! *L! #'K*,#'*?$! @*)HAH$B'50! L&*>!>*?$#! )?',!B$$?#! )H$<%#$!(H!\058!*&!e\03C! =>>?'*@&#/@/%<%#K!GI0! /$! K#%#A%#K! (H! />>?'*()*%! (H! e\03! *&! <'B5II! -K/&#A%#K!<,</'$%!XJ#!I5%#&>/'<)!%</)1!

5 !m

AcTubulin E3

AcTubulin/E3 AcTubulin/E3/DAPI

Collecting duct cells

./,!F2!!=>>?'*E?*&#$A#'A#!K#%#AB*'!*L!#'K*,#'*?$!@*)HAH$B'50!/'!%J#!A/)/<!*L!>*?$#!A*))#AB',!K?A%!A#))$!(H!\FC!

225-

150-

E1, 2 E3, 4

105-

kDa:

1 2 3 1 2 3 1 2 3 1 2 3 1 2 3

E1 E2 E3 E4 7E12