Poster Sessions (November 15 & 16) 29. Protein structure and dynamics (II) 2P001 Characterization of Circularly Permuted Chaperonin GroEL

○Tatsuya Uemura2，Takuma Hirayama1，Kunihiro Hongo1,2，Tomohiro Mizobata1,2，Yasushi Kawata1,2 1Dept.of Biotechnology, Fac. of Engineering, Tottori University 2Graduate School of Medical Sciences, Tottori University

2P002 Plasticity with different strength of GroES amyloid fibril ○Tomohiro Mizobata1，Hisashi Yagi1，Tatsunari Yoshimi1，Isao Sakane1，Kunihiro Hongo1，Yasushi Kawata1 1Department of Biotechnology, Faculty of Engineering, and Graduate School of Medical Science, Tottori University

2P003 Intensive Raman Optical Activity of L-type Straight flagellar Filaments of Salmonella ○Tomonori Uchiyama1，Masashi Sonoyama2，Yoshiaki Hamada3，Fumio Hayashi1，Kenji Oosawa1 1Dept. of Nano-Material Systems, Gunma Univ. 2Dept. of Applied Physics, Nagoya Univ. 3Univ. of the Air

2P004 Vibrational Spectroscopic Implication of the Role of R14 Residue in Human Hematopoietic Prostaglandin D2 Synthase

○Yoshiko Uchida1，Yoshihiro Urade2，Takamitsu Kohzuma1 1Inst. Appli. Beam. Sci., Ibaraki Univ. 2Dept. Mol. Behav., Osaka Bioscience Inst.

2P005 Fourier-transform infrared spectroscopic analysis of the calmodulin binding with peptides of protin 4.1R in physiological solution

○Wataru Nunomura1，Daisuke Sasakura2，Yuichi Takakuwa1 1Dept. of Biochem, Tokyo Women's Medical Univ. 2Bruker Optics K.K.

2P006 Secondary-Structure Analysis of Glycoprotein using Vacuum-Ultraviolet Circular Dichroism Spectroscopy ○Koichi Matsuo1，Kunihiko Gekko1,2 1Synchrotron Radiation Center 2Graduate School of Science, Hiroshima University

2P007 Secondary-Structure Analysis of Denatured Proteins by Vacuum-Ultraviolet Circular Dichroism Spectroscopy ○Yoshie Sakurada1，Koichi Matsuo2，Shin-ichi Tate1，Kunihiko Gekko1,2 1Graduate school of Science, Hiroshima University 2Hiroshima Synchrotron Radiation Center, Hiroshima University

2P008 Secondary-Structure Prediction of Proteins byVacuum-Ultraviolet Circular Dichroism Spectroscopy ○Hidenori Watanabe1，Koichi Matsuo2，Shin-ichi Tate1，Kunihiko Gekko1,2 1Graduate School of Science, Hiroshima University 2Hiroshima Synchrotron Radiation Center, Hiroshima University

2P009 Fluorescence spectroscopic studies on the structure-dynamics-function correlation of ribosome-inactivating proteins. ○Yukihiro Fukunaga1，Etsuko Nishimoto1，Takuhiro Otosu1，Katsumi Yamashita2，Shoji Yamashita1 1Institute of Biophysics, Faculty of Agriculture, Graduate School of Kyushu University 2NEC soft

2P010 Fluorescence spectroscopic study on the CaM-target protein interaction - Interaction with melittin ○Takuhiro Otosu1，Etsuko Nishimoto1，Yukihiro Fukunaga1，Yoshifumi Miyazaki1，Shoji Yamashita1 1Institute of Biophysics, Faculty of Agriculture, Graduate School of Kyushu University

2P011 Unraveling excited state properties of BLUF domain of AppA by photothermal methods ○Partha Hazra1，Keiichi Inoue1，Wouter Laan2，Klaas J Hellingwerf2，Masahide Terazima1 1Department of Chemsitry, Kyoto University 2Swammerdam Institute for Life Sciences, Amsterdam

2P012 Dynamics and Conformational Transition of Protein As Monitored by Dielectric Spectroscopy ○Yoshihito Hayashi1，Ikuya Oshige1，Yoichi Katsumoto1，Shinji Omori1，Akio Yasuda1 1Life Science Laboratory, Materials Laboratories, Sony Corporation

2P013 Multiple conformational changes of Ras observed by single molecule FRET ○Yoshiyuki Arai1，Atsuko H Iwane1，Tetsuichi Wazawa2，Hiroaki Yokota3，Yoshiharu Ishii4，Tohru Kataoka5，Toshio Yanagida1 1Graduate school of Frontier Biosciences, Osaka University 2Graduate school of Engineering, Tohoku University

3Department of Molecular Physiology, The Tokyo Metrropolitan Institute of Medical Science 4Formation of Soft _nanomachines, Core Research for Evolution Science and Technology 5Graduate school of Medicine, Kobe University

2P014 Characterization of actin SS-dimers obtained by oxidation ○Kayoko Serizawa1，Shin'ichi Ishiwata1 1Department of Physics, school of Science and Engineering, Waseda University

2P015 Thermodynamic stability of BPTI variants with highly simplified amino acid sequences ○Atsushi Kato1，Munetaka Yamada1，Shigeyoshi Nakamura2，Shunichi Kidokoro2，Yutaka Kuroda1 1Dept. of Biotech. and Life Sci., Tokyo Univ. of Agric. and Tech. 2Dept. of Bioeng.., Nagaoka Univ. of Tech.

2P016 Dynamic Analysis of Protein Thermal Transition by Differential Scanning Calorimetry ○Shun-ichi Kidokoro1，Asuka Matsumoto1 1Dept. of Bioengineering, Nagaoka Univ. Tech.

2P017 Structure stability of Type IV collagen ○Kenichi Kawai 1，Miki Yanagino1，Akio Shimizu1，Koji Kameyama2，Minoru Machida3，Zenya Naito2 1Department of Engineering, Soka University 2Department of Pathology, Nippon Medical School 3Research Center for Cancer Prevention and Screening, National Cancer Center

2P018 Characteristics of bovine type IV collagen unfolding under various pH conditions ○Miki Yanagino1，Kenichi Kawai1，Akio Shimizu1，Koji Kameyama2，Minoru Machida3，Zenya Naito2 1Department of Engineering, Soka University, Tokyo 2Department of Pathology, Nippon Medical School, Tokyo 3Research Center for Cancer Prevention and Screening, National Cancer Center, Tokyo

2P019 The solubility of amino acids, diglycine, triglycine in aqueous L-Arginine hydrochloride solution. ○Noriyuki Obeyama1，Yosikazu Tanaka1，Daisuke Ejima2，Tsutomu Arakawa3，Kouhei Tsumoto1 1Dept. of Medical genome Sci., Univ. of Tokyo 2Amino Science Laboratories, Ajinomoto, Co. Inc. 3Alliance Protein Laboratories

2P020 Adsorption Reaction Constants of Extremely Diluted Lysozyme in PPBS on the Surfaces of Nanosilica and Nanodiamond

○Victor Wei-Keh Wu1 1Department of Chemical Engineering and Materials Engineering, National Kaohsiung University of Applied Sciences, 80782 Kaohsiung, Taiwan 2Group 510, Institute of Atomic and Molecular Sciences, Academia Sinica, P.O.BOX 23-166, 10617 Taipei, Taiwan 3Victor Basic Research Laboratory e. V., Gadderbaumer-Str. 22, D-33602 Bielefeld, Germany, Email:victorbres3tw@yahoo.com.tw, http://www.che.kuas.edu.tw, Tel:+886-(0)919-300-525

2P021 Direct measurement of interactions between SigB and RsbW proteins ○Yoshiki Nakata1，Takahiro Yamaguchi1，Takehiro Suzuki1，Kazuya Morikawa2，Kazue Kurihara1 1 IMRAM, Tohoku Univ. 2Basic Med., Tsukuba Univ.

2P022 Influence of protein structure on the adsorption of human lysozyme onto hydroxyapatite ○Takuya Nodzumi1，Satoshi Shimamoto1，Daisuke Akieda1，Yasuhiro Nonaka1，Tomoyasu Aizawa1，Makoto Demura2，Keiichi Kawano1 1Grad. Sch. of Sci., Hokkaido Univ. 2Grad. Sch. of Life Sci., Hokkaido Univ.

2P023 Expression and characterization of the human STPR domain: a homologue of the novel silkworm DNA-binding domain

○Takuya Yokoyama1，Shin Saito1，Aizawa Tomoyasu1，Shigeharu Takiya2，Makoto Demura1，Keiichi Kawano1 1Grad. Sch. of Sci., Hokkaiddo Univ. 2Center for Advanced Sci. and Technology, Hokkaido Univ.

2P024 Suggestions for improving diffraction data qualities at the undulator beamlines ○Kunio Hirata1，Nobutaka Shimizu2，Kazuya Hasegawa2，Masaki Yamamoto1,2 1RIKEN SPring-8 Center 2JASRI/SPring-8

2P025 Development of Online UV-Visible Microspectrophotometer for Protein Crystallography at BL38B1 of SPring-8 ○Nobutaka Shimizu1，Kouichi Tomita2，Masao Ogaki3，Kazuya Hasegawa1，Masaki Yamamoto1,4 1JASRI/SPring-8 2PHOTON Design Co. 3EPL Inc. 4RIKEN SPring-8 Center

2P026 Current Status of Public Beamlines for Protein Crystallography at SPring-8 ○Masahide KAWAMOTO1，Kazuya HASEGAWA1，Nobutaka SHIMIZU1，Hisanobu SAKAI1，Kunio HIRATA2，Tetsuya SHIMIZU2，Atsushi NISAWA1，Masaki YAMAMOTO1,2 1SPring-8/JASRI 2SPring-8/RIKEN

2P027 The crystal structure analysis of Aquifex aeolicus methionl-tRNA synethetase ○Kotaro Nakanishi1，Yasuhiro Komoto1，Shuya Fukai1，Osamu Nureki1,2,3 1Dept. of Biological Information, Tokyo Institute of Technology 2PRESTO, JST 3RIKEN Genomic Sciences Center

2P028 Crysatal Structural analysis of HindIII restriction endonuclease in complex with cognate DNA at 2.0 Å resolution ○Chika Sato1，Nobuhisa Watanabe1,2，Yozo Takasaki3，Isao Tanaka1,2 1Div. of Biol. Sci., Grad. Sch. of Sci., Hokkaido Univ. 2Fac. of Adv. Life Sci., Hokkaido Univ. 3Dep. of Biomol. Sci., Fac of Med., Saga Univ.

2P029 Aiming at one molecule measurement of cyanobacteria clock proteins ○Seiji Sakaguchi1，Masahiro Ishiura1,2 1Center for Gene Research, Nagoya Univ. 2Div. of Bio. Sci., Grad. Sch. of Sci., Nagoya Univ.

2P030 Crystallization of KaiA-KaiC complexes for structure analysis ○Kyouhei Bitou1，Ryo Iwase1，Seiji Sakaguchi1，Reiko Murakami1，Katsumi Imada2，Keiichi Namba2，Masahiro Isiura1 1Center for Gene Research, NagoyaUniversity 2Graduce School of Frontier Biosciences, Osaka University

2P031 Crystallization of the catalytic subunit of archaeal oligosaccharyltransferase ○Mayumi Igura1，Nobuo Maita1，Jun Kamishikiryou1，Katsumi Maenaka1，Daisuke Kohda1 1Med. Inst. Bioreg., Kyushu Univ.

2P032 Crystal structures of vitamin D receptor in complex with lithocholic acid derivatives Hiroyuki Masuno1，○Daisuke Morizono1，Teikichi Ikura2，Nobutoshi Ito2，Hector, F DeLuca4，Hirokazu Tamamura1，Masato Shimizu3 1Dept. of Molecular Recognition, Inst. of Biomaterials and Bioengineering, Tokyo Medical and Dental Univ. 2Lab. of Structural Biology, School of Biomedical Science, Tokyo Medical and Dental Univ. 3Lab. of Medicinal Chemistry, School of Biomedical Science, Tokyo Medical and Dental Univ. 4Univ. of Wisconsin

2P033 Structural and Thermodynamic Change of a Fab upon Binding of Human Thrombopoietin ○Shigeki Arai1，Taro Tamada1，Yoshitake Maeda2，Eijiro Honjo1，Ryota Kuroki1 1Molecular Structural Biology Group, Neutron Biology Research Center, Quantum Beam Science Directorate, Japan Atomic Energy Agency 2Pharmaceutical Frontier Laboratory, Kirin Brewery Company Ltd.

2P034 Crystal structure of Atlantic cod trypsin ○Daisuke Iyaguchi1，Haruo Sekizaki1，Midori Tateyama2，Min Yao3，Nobuhisa Watanabe3，Isao Tanaka3，Eiko Toyota1 1Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido 2School of Nursing and Social Services, Health Sciences University of Hokkaido 3Faculty of Advanced Life Sciences, Hokkaido University

2P035 Crystal structure of the complex formed between hSRPK1 and the inhibitor. ○Mitsuteru Hoshina1，Hiroshi Onogi2,3，Masatoshi Hagiwara2,3，Teikichi Ikura1，Nobutoshi Ito1 1Lab. of Structural Biology, School of Biomedical Science, Tokyo Medical & Dental Univ. 2Dept. of Functional Genomics, Medical Research Institute, Tokyo Medical & Dental Univ. 3Lab. of Gene Expression, School of Biomedical Science, Tokyo Medical & Dental Univ.

2P036 The substrate recognition mechanism of flavoenzyme ○Makio Furuichi1，Nobuhiro Suzuki3，Balasundaresan Dhakshnamoorhty2，Yoshihito Yoshida1，Hiroki Kaneko4，Hirotaka MInagawa5，Iwao Waga1，Tadashi Murase1，Hiroshi Mizuno1 1VALWAY Technology Center, NEC Soft, Ltd. 2AIST 3Tsukuba Univ. 4Nihon Univ. 5NEC

2P037 X-ray crystallographic analysis of the receptor uncoupling mutant of Gαi1 ○Tomohito Morikawa1,2，Ayumu Muroya3，Takeshi Tanaka1，Shigetoshi Sugio3，Kaori Wakamatsu2，Toshiyuki Kohno1 1Mitsubishi Kagaku Institute of Life Sciences 2Dept. of Biochem. Chem. Engn., Gunma Univ. 3ZOEGENE corp.

2P038 Structural determination of 13C, 15N-uniformly labeled ATP bound to H+-ATP synthase β subunit by solid-state NMR ○Yasuto Todokoro1，Yoshiyuki Miyasaka2，Hiromasa Yagi1，Masatsune Kainosho3，Toshimichi Fujiwara1，Hideo Akutsu1 1Institute for Protein Research, Osaka University 2Faculty of Engineering, Yokohama National University 3Faculty of

Science, Tokyo Metropolitan University 2P039 Mechanism of Unlimited Product Chain Lengths for Hevea brasiliensis rubber prenyltransferase

Hui-Min Wang1，○Po-Huang Liang1 1Insti. of Bio. Chem, Acade. Sinica, Taipei 11529, Taiwan

2P040 Crystal structure of isocitrate dehydrogenase from Sulfolobus tokodaii ○Hisanori Kondo1，Midori Murakami1，Shin-ichi Suzuki1，Kunio Ihara2，Tsutomu Kouyama1 1Dept. Physics, Graduate School of Science,Nagoya University 2Center for Gene Research, Nagoya University

2P041 X-ray crystal structural analysis of vault from rat liver ○Hideaki TANAKA1，Koji KATO1，Eiki Yamashita1，Chizuko FUJITA1，Tomoyuki SUMIZAWA2，Tomitake TSUKIHARA1 1Institute for Protein Research, Osaka University 2University of Occupational and Environmental Health

2P042 Structural integrity of the isolated manganese stabilising protein from higher plant and cyanobacterial photosystem II ○Adele K. Williamson1，John R Liggins1，Tom Wydrzynski1 1RSBS, ANU

2P043 Conformational changes of human serum albumin observed in single particle electron microscopy ○Yutaka Ueno1，chikara sato1，Kazuhiro Mio1 1Neuroscience, AIST

2P044 Visualization of domain structure of bacterial glucosyltranseferase-I by electron cryo-microscopy ○Norihiko Yaji1，Takuo Yasunaga1，Hideyuki Komatsu1，Takao Kodama1,2 1Department of Bioscience and Bioinfomatics, Faculty of Computer Science and Systems Engineering, Kyusyu Institute of Technology 2Japan Science and Technology Agency

2P045 Clamp-loading mechanism onto DNA revealed by electron microscopy and single particle image analysis ○Hirofumi Suzuki1,2，Tomoko Miyata3，Takuji Oyama1，Kouta Mayanagai4，Yoshizumi Ishino5，Kosuke Morikawa1 1IPR, Osaka Univ. 2CREST, JST 3Grad. Sch. of Front. Biosci., Osaka Univ. 4Dept. of Biosci., Nagahama Inst. of Bio-Sci. and Techn. 5Fac. of Agriculture, Kyushu Univ.

2P046 NMR Structure of Pleckstrin Homology Domain in Phospholipase C-η1 ○Toru Imai1，Mitsuru Tashiro3，Heisaburo Shindo1，Kiyoko Fukami2，Sakurako Tashiro1 1School of pharmacy, Tokyo University of Pharmacy and Life Science 2School of Life Science, Tokyo University of Pharmacy and Life Science 3Faculty of Science, Meisei University

2P047 Prevention of protein aggregation under conditions specific to NMR measurements ○Long Xiang1，Toshihiko Inaoka1，Takashi Ishii1，Kazuo Hosoda1，Kanako Kobori1，Minako Kawaai1，Hidehito Mukai2，Kazuki Sato3，Toshiyuki Kohno2，Kaori Wakamatsu1 1Dept. of Biochem. Chem. Engn., Gunma Univ. 2Mitsubishi Kagaku Institute of Life Sciences 3Dept. of Enviromental Science, Fukuoka Women's Univ.

2P048 Structural Studies on Conserved Hypothetical Protein HP0894 and its Complex from Helicobacter pylori ○Atsushi Matsuura1，Kyung-Doo Han1，Sung-Jean Park1，Sun-Bok Jang1，Woo-Sung Son1，Bong-Jin Lee1 1College of pharmacy, Seoul National Univ.

2P049 NMR study on the interaction of the transactivation domain of ATF-2 with MAP kinase p38 ○Aritaka Nagadoi1，Ishizu Michiko1，Yoshifumi Nishimura1 1International Graduate School of Arts and Sciences, Yokohama city Univ.

2P050 Structural Analysis of G-Protein-Bound Mastoparan-X by Solid-State NMR ○Makiko Hori1，Yasuto Todokoro1，Ikuko Yumen1，Toshiyuki Kohno2，Kaori Wakamatsu3，Toshimichi Fujiwara1，Hideo Akutsu1 1Institute for Protein Research, Osaka University, Suita 2Mitsubishi Kagaku Institute of Life Sciences (MITILS), Machida

3Department of Biochemical and Chemical Engineering, Gunma University, Kiryu 2P051 Differences between solution and crystal structures of a DNA binding protein

○Tsutomu Yamane1，Hideyasu Okamura1，Mitsunori Ikeguchi1，Akinori Kidera1，Yoshifumi Nishimura1 1Int. Grad. sch. of Arts and Sci., Yokohama City Univ.

2P052 Solution Structure of Variable-type Domain of Receptor for Advanced Glycation Endproducts ○Shigeyuki Matsumoto1，Takuya Yoshida1，Hiroko Murata1，Hiroshi Yamamoto2，Yuji Kobayashi1,3，Tadayasu Ohkubo1 1Grad. Sch. of Pharm. Sci., Osaka Univ. 2Grad. Sch. of Med. Sci., Kanazawa Univ. 3Osaka Univ. of Pharm. Sci.

2P053 Structural basis and DNA binding property of α domain from Bacillus subtilis Lon protease ○Iren Wang1,3，Yuan-Chao Lou1，Yu-Ching Lin2,3，Shih-Chi Lo1，Shih-Hsiung Wu2,3，Chinpan Chen1 1Institute of Biomedical Sciences, Academia Sinica 2Institute of Biological Chemistry, Academia Sinica 3Institute of Biochemical Sciences, National Taiwan University

2P054 Solution Structure of Family 21 Carbohydrate-binding Module from Rhizopus Oryzae Glucoseamylase ○Ping-Chiang Lyu1,2，Yu-Nan Liu2，Wei-I Chou2，Dah-Thyr Chang1,3 1Institute of Bioinformatics and Structural Biology, National Tsing Hua University 2Department of Life Science, National Tsing Hua University 3Institute of Molecular and Cellular Biology, National Tsing Hua University

2P055 Structure of a Polymyxin B Resistant Protein (PmrD) and its complex formation when interacting with PmrA from Klebsiella pneumoniae

○Shih-Chi Luo1,2，Yuan-Chou Lou1， Chinpan Chen1,2 1Institute of Biomedical Science, Academia Sinica 2Taiwan International Graduate Program

2P056 The flexible and clustered lysine residues are critical for membrane permeability and antimicrobial activity of human RNase 7.

○Yu-Chie Huang1,2,3，Yuan-Chou Lou1，Yu-Min Lin1，You-Di Liao1，Shih-Hsiung Wu2,3，Chinpan Chen1 1 Inst. of Biomedical Sciences , Academia Sinica 2Inst. of Biological Chemistry, Academia Sinica 3Inst, of Biochemical Sciences, NTU

2P057 Variable pressure NMR reveals role of cavity in protein dynamics ○Akihiro Maeno1，Ryo Kitahara2，Frederick W. Dahlquist3，Shigeyuki Yokoyama4，Frans A. A. Mulder5，Kazuyuki Akasaka6

1Dept. of Biotech, Graduate school of Kinki Univ. 2RIKEN Harima Institute 3Institute of Molecular Biology and Dept. of chem, Oregon Univ. 4Dept. of Biophys and Biochem, Graduate School of Sci, Tokyo Univ. 5Dept. of Biophys Chem, Lund Univ 6Dept. of Biotech, Graduate school of Biology-Oriented Sci and Tech, Kinki Univ.

2P058 Dual Binding Sites for Translocation Catalysis by E. coli Glutathionylspermidine Synthetase ○Chien-Hua Pai1,3，Bing-Yu Chiang1,4，Tzu-Ping Ko1，Chia-Cheng Chou2，Cheong-Meng Chong1，Fang-Jiun Yen1，James K. Coward5，Chun-Hung Lin1,2,4，Andrew H.-J. Wang1,2,4 1Institute of Biological Chemistry, Academia Sinica 2Genomics Research Center, Academia Sinica 3Institute of Biochemistry, National Yang-Ming University, Taiwan 4Institute of Biochemical Sciences, College of Life Science, National Taiwan University,Taiwan 5Dept. of Chem., Univ. of Michigan

2P059 On the Micro-crystal in the Photophore at Fourth Arms of Firefly Squid ○Toshiaki Hamanaka1，Masatsugu Seidou2，Masanao Michinomae3，Kenji Hiraki4，Yuji Kito5 1Dept. of Mechanical Science and Bioengineering., Grad. School of Engineering Science, Osaka Univ. 2Dept. of General Education, Aichi Prefectural Univ. of Fine Arts and Music 3Dept. of Biology., Facul. of Science and Engineering, Konan Univ. 4Suntory Institute for Bioorganic Research 5TYK Lab. for Photobiology

2P060 Solution Structure of the Bromodomain of Brd2 and Its Interaction with Acetylated Peptide from Histone H4 and CtBP

Hongda Huang1,2，Chao Xu1,2，Jiahai Zhang1,2，Haiming Dai1,2，Jihui Wu1,2，○Yunyu Shi1,2 1Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China 2School of Life Science, University of Science and Technology of China

30. Protein function (II) 2P061 ATP-dependent interactions between Escherichia coli MinC/MinD complex and phospholipid membrane

○Takashi Okuno1，Toshihiko Sakurai2，Mayumi Maruyama1，Shun Hirota1,3，Noriaki Funasaki1 1Dept of Phys. Chem., Kyoto Pharm. Univ. 2Dept. of Biotech., Faculty of Eng., Tottori Univ. 3PRESTO, JST

2P062 Expression and functional analysis of lipocalin super-family proteins in the olfactory epithelium of the newt ○Mandula Glaqin1，Tsukasa Takahashi1，Ken Sawada1，Tatsuo Iwasa1 1Dept. of Mat. Sci. and Eng., Muroran IT

2P063 Expression and purification of bacteriophage T5 A1 and A2 proteins are related with viral DNA injection ○Tomoko Nakao1,2，Laure Plancon1，Lucienne Letellier1 1Institut de Biochimie et Biophysique Moleculaire et Cellulaire, Universite de Paris-Sud 2Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology

2P064 Dual Functional Property of non-heme iron containing ferritin Pfr in Helicobacter pylori under acid stress ○I-Ju Yeh1，Shyh-Horng Chiou2 1Dept. of Biochemistry, National Taiwan University 2Dept. of Biochemistry, National Taiwan University

2P065 Synchronization of a circadian clock in vitro ○Hiroshi Ito1，Michinori Mutsuda1，Hakuto Kageyama1，Tokitaka Oyama1，Takao Kondo1 1Div. of Biological Science, Nagoya Univ.

2P066 The biochemical function of the cyanobacterial circadian clock protein KaiB ○Risa Mutoh1，Reiko Murakami1，Ryo Iwase1，Masahiro Ishiura1 1 Center for Gene Research, Nagoya Univ.

2P067 The function of circadian clock Kai proteins in the cyanobacterium Synechocystis sp. strain PCC 6803 ○Reiko Murakami1，Masahiro Ishiura1 1From Center for Gene Research, Nagoya Univ.

2P068 The general relationships between the three dimensional structures andelectronic wave functions of proteins ○kentaro fukushima1，minoru sakurai2 1tokyo institute of technology 2tokyo institute of technology

2P069 Computational Study of Catalytic Reaction of Adenylate Kinase ○Kenshu Kamiya1，Shinya Morishita1，Yoko Sugawara1 1Dept. of Physics, School of Science, Kitasato Univ.

2P070 Selective ion-binding by protein probed with the statistical mechanical integral equation theory ○Norio Yoshida1，Saree Phongphanphanee1，Fumio Hirata1 1Institute for molecular science

2P071 The Ligand-Binding Mechanism of the Glutamine Binding Protein ○Hannes H. Loeffler1，Akio Kitao1 1Laboratory of Molecular Design, Center for Bioinformatics, Institute of Molecular and Cellular Biosciences, The University of Tokyo

2P072 Quantum chemical study of the hydrolytic reaction of polylactic acid by the enzyme degrading biodegradable plastics Yoshitake Sakae1，Toshiaki Matsubara1，○Misako Aida2，Hidemasa Kondou3，Kazuo Masaki4，Haruyuki Iefuji4 1QuLiS, Hiroshima Univ. 2Dept. of Chemistry, Hiroshima Univ. 3Res. Inst. of Genome-based Biofactory, AIST

4Environmental Res. Div., NRIB 2P073 Quantitative analysis of conservation of amino acid residues critical for protein-protein interaction.

○Hiroyuki Oda1，Hiroyuki Toh1，Kazutaka Katoh1 1Mib, Kyushu Univ.

2P074 Systematic Analyses of Catalytic Mechanisms of Hydrolysis and Transfer Reactions ○Nozomi Nagano1 1CBRC-AIST

2P075 Structural Change of Lipocalin-type Prostaglandin D Synthase Studied with Small Angle X-ray Scattering ○Katsuaki Inoue1，Takashi Inui2,3，Tadayasu Okubo4，Keigo Goda5，Naoto Yagi1 1SPring-8/JASRI 2Osaka Prefecture University 3Osaka Bioscience Institute 4Osaka University 5Computer-Aided Molecular Modeling Research Center Kansai

2P076 Structural Analysis of GGA (VHS-GAT) -Arf Complex Using SAXS Measurement

○Kazumi Hirako1，Hironari Kamikubo1，Masato Kawasaki2，Ryuichi Kato2，Kazuhisa Nakayama3，Soichi Wakatsuki2，Mikio Kataoka1 1Graduate School of Materials Science, Nara Institute of Science and Technology 2Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK) 3Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University

2P077 NMR analysis of interaction between Vasoactive Intestinal Peptide and the extracellular domain of VIP receptor. ○Machiko Iwata1，Natsuko Goda1，Takeshi Tenno2，Hidekazu Hiroaki1 1International Graduate School of Arts and Sciences,Yokohama City University 2Graduate School of Molecular engineering, Kyoto University

2P078 Interactions between Dynorpin and Extra Celler Loop II of κ-Opioid Receptor as studied by Solid State NMR ○Masayuki eguchi1，Takenori Miyamori1，Takahiro Sakai1，Akira Naito1，Katsuyuki Nishimura2，Atsushi Kira3 1Sch.Eng.,Yokohama Natl. Univ., 2Institute for Moleculer Science 3ULVAC Ltd.

2P079 Experimental Reflection on Conformational Transition of Poly(proline) Mitsuhiro Yuge1，Sachiro Kakinoki1，Yoshiaki Hirano2，○Masahito Oka1 1Faculty of Liberal Arts & Sciences, Osaka Prefecture University 2Department of Applied Chemistry, Osaka Institute of Technology

2P080 Cancel 2P081 Importance of b-sheet structure for heat stability of Ca+2 saturated calmodulin: infrared spectroscopic analysis in physiological solution

○Daisuke Sasakura1，Nunomura Wataru2，Takakuwa Yuichi2 1Bruker Optics K.K. 2Dept. of Biochem, Tokyo Women's Medical University

2P082 Spectroscopic and Electrochemical Studies of a Blue Copper Protein, Pseudoazurin Thr36X mutants ○Rehab Abdelhamid1，Tomoko Aono1，Takamitsu Kohzuma1 1Ibaraki University

2P083 Kinetic study on binding of Fe(II) to transferrin and formation of Fe(III)-transferrin complex ○Tetsuya Sakajiri1,2，Takaki Yamamura2，Takeshi Kikuchi3，Hirofumi Yajima1 1Dept. of App. Chem, Tokyo Univ. of Sci. 2Dept. of Food and Nutrition, Morioka College 3Dept. of BioSci. Bioinfo. Ritsumei. Univ.

2P084 Structural change of protein described by linear response theory : internal coordinates ○Satoshi Omori1，Sotaro Fuchigami1，Mitsunori Ikeguchi1，Akinori Kidera1 1Itn. Grad. School of Arts and Sciences, Yokohama City Univ.

2P085 Phosphorylation-induced conformational change of p47phox: CW ESR and Four-Pulse DEER study by a site-directed spin labeling (SDSL)

○Wakako Hiraoka1，Tomoko Suzuki1，Hideyuki Hara2，Mikinori Kuwabara3，Osamu Inanami3 1Dept. of Physics, School of Sci. and Tech., Meiji Univ. 2ESR Application, Bruker BioSpin K.K. 3Graduate School of Vet. Med. Sci, Hokkaido Univ.

2P086 Change in Optical Properties of Stratum Corneum Induced by Keratin Carbonylation ○Ichiro Iwai1，Kaori Ikuta1，Koichi Murayama2，Tetsuji Hirao1 1Shiseido Research Center, SHISEIDO Co., Ltd. 2Department of Biochemistry and Biophysics, Gifu University

2P087 Hydration Structure Change of Protein upon Ligand Induced Domain Closure ○Akio Kitao1,2，Steven Hayward3 1Inst. Mol. Cell. Biosci., Univ. Tokyo 2CREST, JST 3Sch. Comput. Sci. and Sch. Biol. Sci., Univ. East Anglia

2P088 Effect of pH and ionic concentration on membrane lysis of dimyristoylphosphatidylcholine vesicles by α-Lactalbumin ○Jun Okuda1，Masashi Sonoyama1，Shigeki Mitaku1 1Dept. of Applied Physics, Nagoya Univ.

2P089 NMR Studies of Virulence-associated Proteins and Small Conserved Hypothetical Proteins in Klebsiella Pneumoniae ○Kuo-Wei Hung1，Chun-Chia Cheng1，Yi-Chao Lin1，Tsan-Hung Yu1，Pei-Ju Fan2，Chi-Fon Chang2，Shih-Feng Tsai3，Tai-Huang Huang1,2 1Inst. Biomed. Sci., Academia Sinica, Taipei, Taiwan, ROC 2Genomic Research Center, Academia Sinica, Taipei, Taiwan, ROC 3Div. Molecular & Genomic Medicine, National Health Research Institute, Zhunan, Miaoli,

2P090 Development of a novel high efficient purification method and observation by TEM of recombinant soluble human immunosuppressive protein

○Noriyuki Ishii1，Hiroaki Takagawa2，Sachi Fujishima1，Hisamune Kato2，Pi-Chao Wang2 1Biol. Info. Res. Center, AIST 2Grad. Sch. of Life and Environ. Sci., Tsukuba Univ.

2P091 Turing metallothionein into magnetic protein ○Chia-Ching Chang1,2,3，Shang-Fan Lee2，Kein-Wen Sun4，Lou-Sing Kan5 1Dept. of Bio. Sci. & Tech., National Chiao Tung Univ. 2Insitute of Physics, Academia Sinica 3National Nano Device Laboratories 4Dept. Appl. Chem. & Inst. of Mol. Sci. National Chiao Tung Univ. 5Inst. of Chem. Academia Sinica

31. Protein folding and misfolding (II) 2P092 Elucidation of the unfolding-state and the folding process of the disulfide-bond introduced mutant of Staphylococcal nuclease

○Shingo Kato1，Hironari Kamikubo1，Masayoshi Onitsuka1，Yoichi Yamazaki1，Yasushi Imamoto1，Mikio Kataoka1 1Graduate School of Materials Science, Nara Institute of Science and Technology

2P093 Volume Profile Analysis of Cytochrome c Folding by High Pressure Spectroscopy ○Akiko Ehara1，Koichi Sakamoto1，Koichiro Ishimori1

1Div. of Chemistry, Grad. Sch. of Science, Hokkaido Univ. 2P094 Localization of Prefoldin Interaction Sites in Group II Chaperonin and Correlations between Binding Rate and Protein Transfer Rate

○Yosuke Murase1,2，Tomatsu Zako1,2，Ryo Iizuka1,4，Takao Yoshida3，Taro Kanzaki1，Masafumi Yohda1 1Tokyo University of Agriculture and Technology 2RIKEN 3JAMSTEC 4The University of Tokyo

2P095 Argininamide: New class of additive that improves the refolding yield of lysozyme. ○Hiroyuki Hamada1，Kentaro Shiraki1 1Grad. Sch. of Pure and Appl. Sci., Univ. of Tsukuba,

2P096 Molecular characterization of prefoldins from a hyperthermophilic archaeon, Thermococcus sp. strain KS-1 ○Yuri Sugano1，Ryo Iizuka1,2，Akashi Ohtaki1，Naoki Ide1，Takao Yoshida3，Shinsuke Fujiwara4，Tadayuki Imanaka5，Masafumi Yohda1 1Grad. School of Technol., Tokyo Univ. of Agric. and Technol. 2Grad. School of Pharmaceutical Sciences., The University of Tokyo. 3Extremobiosphere Research Center, JAMSTEC 4Dept. of Bioscience School of Science and Technol., Kwansei Gakuin Univ. 5Grad. School of Engineering, Kyoto Univ.

2P097 Molecular chaperone prefoldin prevents amyloid beta fibrillation but produces highly toxic soluble oligomer ○Masafumi Sakono1，Tamotsu Zako1，Hiroshi Ueda2，Masafumi Yohda3，Mizuo Maeda1 1RIKEN, Bioengineering lab 2Dept. Chem. Biotech., Grad. Sch. Eng., Univ. Tokyo 3Dept. Biotechnol., Tokyo Univ. Agric. Technol.

2P098 Comparative analysis of low-molecular weight additives for refolding and aggregation ○Koji Matsumoto1，Tsuneyoshi Matsuoka1，Hiroyuki Hamada1，Kentaro Shiraki1 1Grad. Sch. of Pure and Appl. Sci., Univ. of Tsukuba

2P099 Energy landscape of folding proteins revealed by large-scale multicanonical fragment assembly simulations ○Naoto Hori1，George Chikenji2，Shoji Takada3 1Graduate School of Science and Technology, Kobe University 2Graduate School of Engineering, Nagoya University

3Faculty of Science, Kobe University 2P100 Structural Characterization of Sup35 Amyloid Fibrils

○Aiko Kishimoto-Okada1，Fadel A Samatey2，Kazuya Hasegawa3，Hideki Taguchi4，Masasuke Yoshida5，Keiichi namba1,2 1Graguate School of Frontier Biosciences, Osaka Univ. 2Dynamic NanoMachine Project, ICORP, JST 3SPring-8, JASRI

4Graguate School of Frontier Sciences, Tokyo Univ. 5Chemical Resources Laboratory, Tokyo Institute of Technology 2P101 Biochemical modification of pathological protein aggregation

○Takashi Konno1，Akiyoshi Hirata2，Shigetoshi Oiki1，Koji Ikura3，Takashi Morii2 1Dept of Biophysics, Univ. Fukui 2Inst. of Advanced Energy, Kyoto Univ. 3Dept. of Applied Biology, Kyoto Inst. of Tech.

2P102 Is ALS an amyloid disease? - Molecular mechanism of SOD1 fibrillization ○Yoshiaki Furukawa1，Nobuyuki Nukina1 1Structural Neuropathology Group, BSI, RIKEN

2P103 Characterization of flexible insulin fibrils induced by reducing agent ○Tamotsu Zako1，Masafumi Sakono1，Naomi Hashimoto1，Masaki Ihara1，Mizuo Maeda1 1RIKEN, Bioengineering Lab.

2P104 Structural implications of an amyloidogenic sequence attached to a folded protein ○Daizo Hamada1,2，Kouhei Tsumoto3，Naoki Tanaka4，Kentaro Shiraki5，Itaru Yanagihara1 1Dept of Develop Infect Dis, Res Inst, Osaka Med Cent for Matern and Child Health 2Inst for Prot Res, Osaka Univ 3Dept of Med Genome Sci, Grad Schl of Front Sci, Univ of Tokyo 4Dept of Polym Sci & Eng, Kyoto Inst of Tech 5Inst of Appl Phys, Univ of Tsukuba

2P105 Structural polymorphism of β2-microglobulin amyloid fibrils induced by the addition of trifluoroethanol ○Eri Chatani1，Hisashi Yagi1，Hironobu Naiki2，Yuji Goto1 1Institute for Protein Research, Osaka Univ. and CREST 2Faculty of Medical Sciences, Univ. of Fukui and CREST

2P106 Orientation of amyloid fibrils formed by β2-microglobulin and its peptide fragment in a flow ○Rumi Adachi1，Kei-ichi Yamaguchi1，Miyo Sakai1，Hisashi Yagi1，Kazumasa Sakurai1，Hironobu Naiki2,3，Yuji Goto1,3 1Inst. Protein Res, Osaka Univ 2Fac. of Med Sci, Fukui Univ 3CREST/JST

2P107 Conformational transitions and fibrillation mechanism of hCT peptide DFNKF as studied by high-resolution 13C solid-state NMR

○Kengo Daidoji1，Miya Kamihira2，Tsutomu Nakayama2，Akira Naito1 1Grad. Sch. Eng, Yokohama Natl. Univ. 2Dept. Food and Nutritional Sci, Univ. Shizuoka

2P108 Facile formation of amyloid-like fibrils of hen lysozyme disulfide-variants involves the carboxyl-terminal hydrophobic peptide region

○Hideki Tachibana1，Mitsunobu Yusa2，Hiroki Shimoura1，Shinko Nakamura3，Minoru Kato3，Shin-ichi Segawa2 1Dept. Biology, Kobe Univ. 2Dept. Physics, , Kwansei Gakuin Univ. 3Dept. Applied Chemistry, Ritsumeikan Univ.

2P109 Folding and Unfolding Kinetics of β2-Microglobulin as Probed by Tryptophan Fluorescence ○Michiko Sakata1，Eri Chatani1，Atsushi Kameda1，Kazumasa Sakurai1，Hironobu Naiki2，Yuji Goto1 1Inst. Protein Res., Osaka Univ. and CREST 2Fac. Med. Sci., Fukui Univ. and CREST

2P110 Polymerization and folding of neuroserpin: implication for the conformational disease FENIB ○Sayaka Takehara1，Tomoyoshi Mita1，Maki Onda1 1Dept. of Biol. Sci., Grad. Sch. of Sci.,Osaka Prefecture University

2P111 Fibril formation and structure of glucagon as studied by 13C solid-state NMR spectroscopy Hideki Fujita1，Eri Yoshimoto1，Izumi Yamane1，○Akira Naito1 1Graduate School of Engineering, Yokohama National University

2P112 Heat Capacity Change Associated with Structural Conversion into Amyloid Fibril ○Kenji Sasahara1,3，Hisashi Yagi1，Hironobu Naiki2,3，Yuji Goto1,3 1IPR, Osaka Univ. 2Med. Sci, Fukui Univ. 3CREST

2P113 Fluorescence correlation spectroscopy detects polyglutamine small oligomers increased by knock-down of cytosolic chaperonin CCT.

○Akira Kitamura1,6，Hiroshi Kubota1,6，Chan-Gi Pack2，Gen Matsumoto3，Shoshiro Hirayama1,6，Yasuo Takahashi2,4，Hiroshi Kumura5，Masataka Kinjo2，Richard_I. Morimoto3，Kazuhiro Nagata1,6 1Institute for Frontier Medical Scineces, Kyoto University 2Research Institute for Electronic Science, Hokkaido University

3Northwestern University 4Olympus Corporation 5HMRO, School of Medicine, Kyoto University 6CREST/JST 2P114 Amyloid fibril formation of a peptide fragment of transthyretin

○Makoto Takeuchi1，Mineyuki Mizuguchi1，Mizuki Dobashi1，Yoshihiro Mori1，Hiroyuki Shinoda1，Keiichi Kawano2 1Fac. of Phar. Sci., Toyama Univ. 2Div. of Biol. Sci., Grad. Sch. of Sci., Hokkaido Univ.

2P115 Proline-scanning mutagenesis of transthyretin and its effect on the intermediate structure. ○Yukari Nagai1，Mineyuki Mizuguchi1，Daisuke Saitoh1，Makoto Takeuti1，Yuko Nabeshima1，Yoshinori Mori1，Hiroyuki Shinoda1，Keiichi Kawano2 1Fac. of Phar.Sci.,Univ. of Toyama 2Div. of Biol. Sci., Grad. Sch. of Sci., Hokkaido Univ.

2P116 Transthyretin Mutations and Neurotoxicity ○Minori Soejima1，Mineyuki Mizuguchi1，Kohei Igarashi1，Yuko Nabeshima1，Yoshihiro Mori1，Hiroyuki Shinoda1，Keiichi Kawano2 1Fac. of Phar. Sci., Univ. of Toyama 2Div. of Biol. Sci., Grad. Sch. of Sci., Hokkaido Univ.

2P117 Effect of the sequence in a tail region on amyloid formation ○Hisayuki Morii1，Tomoko Okada1 1Natl. Inst. Adv. Ind. Sci. Technol. (AIST)

2P118 A Microbeam X-ray Diffraction Study on Insulin Spherulites ○Naoto Yagi1，Noboru Ohta1，Tohko Iida1，Katsuaki Inoue1 1Japan Synchrotron Radiation Research Institute, SPring-8

2P119 Reduction of disulfide bridge inhibits the amyloid fibril formation of β2-microglobulin ○Kaori Yamamoto1，Hisashi Yagi1，Kazuhiro Hasegawa2，Hironobu Naiki2,3，Yuji Goto1,3 1Inst. Protein Res., Osaka Univ. 2Fac. Med. Sci., Fukui Univ. 3CREST

2P120 The effect of trehalose on the aggregation of β-amyloid ○Asako Watanabe1，Minoru Sakurai1 1Center for Biol. Res. Info., Tokyo Inst. Tech

2P121 Identification of potential amyloid-forming sequence in prion proteins ○Masatoshi Saiki1，Yuji Hidaka1，Hisayuki Morii2 1Department of Life Science, School of Science and Engineering, Kinki University 2National Institute of Advanced Industrial Science and Technology (AIST)

2P122 Amyloid fibril formation from wild-type hen egg-white lysozyme ○Itsuho Hayashi1，Akihiro Maeno1，Kazuyuki Akasaka1 1Dept. of Biotechnological Science, Kinki Univ

2P123 The disulfide-bond dependence of the morphology of amyloid-like fibers from hen lysozyme ○Ryohei Kono1，Hideki Tachibana2，Kazuyuki Akasaka1 1Department of biotechnological sciences, school of biology-oriented science and technology, Kinki university 2Department of Biology, Faculty of Science and Graduate School of Science and Technology, Kobe University

2P124 Probing energy landscape of ubiquitin-like proteins with variable-pressure NMR ○Ryo Kitahara1，Shigeyuki Yokoyama1,2,3，Kazuyuki Akasaka1,4，Koichi Kato2,5,6 1RIKEN SPring-8 Center 2RIKEN Genomic Sciences Center 3The University of Tokyo 4Kinki University 5Nagoya City University 6Molecular Scale Nano-Science Center, Institute for Molecular Science

2P125 Relationship between pH-dependent conformational and stability changes of bovine β-lactoglobulin studied using NMR spectroscopy

○Kazumasa Sakurai1，Yuji Goto1 1Inst. Protein Res., Osaka Univ.

2P126 Characterization of oligomeric human prion protein (23-231) by 1H-NMR ○Ken Sasaki1，Jyoti Gaikwad1，Show Kitamoto2，Shuhei Hashiguchi2，Kazuhisa Sugimura2，Kazuyuki Akasaka1 1Department of Biotechnological Sciences, School of Biology-Oriented Science and Technology, Kinki University

2Department of Bioengineering, Faculty of Engineering, Kagoshima University, Japan 2P127 SUMO-3 (Small Ubiquitin-like Modifier) Recognition by a SUMO Binding Motif

○Naotaka Sekiyama1，Toshihito Saitoh2，Takahisa Ikegami3，Hidehito Tochio1，Masahiro Shirakawa1 1Department of Molecular Engineering, Kyoto University 2Department of Regeneration Medicine, Institute of Molecular Embryology and Genetics, Kumamoto University 3Institute for Protein Research, Osaka University

2P128 On the origin of the slow and long-lasting oscillation exhibited by a single green fluorescent protein ○Hidetoshi Morita1，Mitsunori Takano1 1Facul. of Sci. & Eng., Waseda Univ.

2P129 Binding of Local Anesthetics to Bovine Serum Albumin ○Yuya Masai1，Kie Kubouchi2，Kazuhito Nagayama2，Takashi Hata2，Hiromu Satake3，Hitoshi Matsuki4，Shoji Kaneshina4 1Advanced Course in Materials Science and Engineering, Kochi National College of Technology 2Department of Material Science and Technology, Kochi National College of Technology 3Center for Cooperative Research, The University of Tokushima 4Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima

2P130 Structural analysis of fibril-forming Alzheimer's amyloid-β ○Kensei Yoshida1,3，Masayuki Nara2，Takeo Konakahara1，Tomoko Okada3，Hisayuki Morii3 1Faculty of Science and Technology, Tokyo Univ. Sci. (RIKADAI) 2Dept. of Chemistry, Tokyo Medical and Dental Univ. (TMDU) 3Natl. Inst. Adv. Ind. Sci. Technol. (AIST)

2P131 Theoretical model of transmembrane prion misfolding: Cooperativity enhances the contact-induced structure transformation

○Chi-Ming Chen1，DM Ou1，CC Chen1 1National Taiwan Normal University

32. Evolutionary molecular engineering 2P132 In vitro selection of functional proteins from random-sequence libraries with reduced alphabets

○Junko Tanaka1，Nobuhide Doi1，Hideaki Takashima1，Hiroshi Yanagawa1 1Grad. Sch. Sci. & Tech., Keio Univ.

2P133 Creation of novel proteins by block shuffling ○Michiko Onimaru1，Toru Tsuji1，Hiroshi Yanagawa1 1Sch. Sci & Tech., Keio Univ.

2P134 Nascent chain, RNA, and ribosome complexes generated by reconstituted in vitro translation system ○Hayato Yanagida1，Tomoaki Matsuura2，Junya Ushioda2，Itaru Urabe2，Tetsuya Yomo1,3,4 1Grad. Sch. Fron. Biosci., Osaka Univ. 2Dept. Biotech., Grad. Sch. Eng., Osaka Univ. 3Dept. Bioinfo. Eng., Grad. Sch. IST, Osaka Univ. 4JST ERATO

2P135 Evolution of Promoter in Natural Selection-type Evolution Reactor ○Yasuo Ageno1，Hiroki Nagayasu1，Naofumi Yamada1，Yuzuru Husimi1 1Dept. of Functional Materials Sci., Saitama Univ.

2P136 An in vitro display method for evolution of the protein having free C-teminus ○Shingo Ueno1，Hidenao Arai1，Miho Suzuki1，Yuzuru Husimi1 1Dept. of Functional Materials Sci, Saitama Univ.

2P137 Efficient genome construction method for a new species of in vitro virus ○Hidenao Arai1，Shingo Ueno1，Miho Suzuki1，Yuzuru Husimi1 1Dept. of Functional Materials Sci, Saitama Univ.

2P138 A novel biased mutation assembling method for evolutionary protein engineering ○Takeru Suzuki1，Takuyo Aita2，Rui Futatsugi2，Yuzuru Husimi1 1Dept. of Functional Materials Sci, Saitama Univ. Japan 2REDS Group

2P139 On-chip Evolution: Overcoming of fluctuation in analysis of a single molecule activity in a pico-litter well ○Naoto Nemoto1,2，Manish Biyani1，Yousuke Hosoi3，Takanori Ichiki3 1REDS Group (CREATE, JST) 2Janusys Corp 3Inst. of Eng. Innovation, Univ. of Tokyo

2P140 Directed evolution of IRES for promoting cap-independent cell-free Translation system ○Manish Biyani1，Madhu Biyani1，Naoto Nemoto1,2 1REDS/JST, Saitama Small Enterprise Promotion Corporation, Saitama 2Janusys Corp., Saitama

33. Redox enzymes 2P141 Conserved residue Arg-193 at the catalytic site of plant sulfite reductase plays an important role for the specific substrate reduction.

○Masato Nakayama1，Genji Kurisu2，Masami Kusunoki1，Toshiharu Hase1 1Inst. for Protein Res., Osaka Univ. 2Grad. School of Arts and Science, Univ. of Tokyo

2P142 Dynamic structure of xanthine oxidoreductase by normal mode analysis ○Hiroto Kikuchi1，Noboru Watanabe2，Ken Okamoto3，Silke Leimkuler4，Takeshi Nishino3 1Dept. of Phys., Nippon Med. Sch. 2Center for Information Sciences, Nippon Med. Sch. 3Dept. of Biochem., Nippon Med. Sch. 4Dept. of Plant Biology, Technical Univ. Braunschweig

2P143 First crystal structure of the 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase ○Neratur K. Lokanath1，Mitsuhiro Nakamura1，Noriyasu Ohshima1，Koji Takio1，Naoki Kunishima1 1RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148,

2P144 Four-domain arrangements of Thermophilic Fungal protein disulfide isomerase in redox state revealed by small-angle X-ray scattering

○Toshihiko Oka1，Aya Maeno2，Eiji Kurimoto2，Osamu Asami3，Tsutomu Kajino3，Koichi Kato2，Masayoshi Nakasako1 1Faculty of Science and Technology, Keio University 2Graduate School of Pharmaceutical Sciences, Nagoya City University

3Toyota Central Research & Development Labs. Inc., Nagakute 2P145 Redox-dependent conformational rearrangement of the substrate-binding domains of protein disulfide isomerase

○Aya Maeno1,2，Toshihiko Oka3，Jun Kikuchi4，Michiko Nakano1,2，Hiroaki Sasakawa 1,2，Eiji Kurimoto1，Yoshiki Yamaguchi1，Osamu Asami5，Tsutomu Kajino5，Masayoshi Nakasako3，Koichi Kato1,2 1Graduate School of Pharmaceutical Sciences, Nagoya City University 2Institute for Molecular Science 3Faculty of Science and Technology, Keio University 4Plant Science Center, Japan, RIKEN 5Toyota Central Research & Development Labs

2P146 Structural and Biochemical Characterization of Thioredoxin Reductase from Dictyostelium discoideum ○Sun-Young Jeong1，In-Kwon Kim1，Sa-Ouk Kang1 1Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University

2P147 Glutathione is an Important Factor in the Development of Dictyostelium discoideum ○Chang-Hoon Choi1，Beom-Jun Kim1，Sa-Ouk Kang1 1Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University

2P148 Role of Aldose Reductase in Dictyostelium discoideum ○Seong-Jun Park1，Chang-Hoon Choi1，Sa-Ouk Kang1 1Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul

2P149 A novel supramolecular assembly and intermolecular electrontransfer process of Hyphomicrobium Cu-containing nitrite reductase

○Tsuyoshi Inoue1，Young Xie1，Yasushi Kai1，Masaki Nojiri2，Kazuya Yamaguchi2，Shinnichiro Suzuki2 1Department of Materials Chemistry, Graduate School of Engineering, Osaka University 2Department of Chemistry, Graduate School of Science, Osaka University

34. Membrane proteins 2P150 Crystallization of Bacterial Nitric Oxide Reductase Using Monoclonal Antibody Fragment.

○Tomoya Hino1，Hideyuki Kumita1，Yoshihiro Fukumori2，Yoshitsugu Shiro1 1RIKEN SPring-8 Center 2Dept. of Biol., Fac. of Sci., Kanazawa Univ

2P151 Crystallization and preliminary X-ray analysis of the bacterial membrane transporters ○Motoyuki Hattori1，Yoshiki Tanaka1，Shuya Fukai1，Ryuichiro Ishitani1，Osamu Nureki1,2 1Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology

2Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency 2P152 Crystallization and X-ray crystallographic study of squid rhodopsin

○Midori Murakami1，Tsutomu Kouyama1 1Dept. of Physics, Nagoya Univ.

2P153 The Structure of central rotor in V type ATPase/synthase ○Ken Yokoyama1 1JST ERATO ATP system 2Tokyo Institute of Technology

2P154 Intra-membrane particles of Na+/K+-ATPase observed by quick-freezing replica technique ○Kazunori Kawasaki1，Emiko Kobayashi1，Kazuhiro Aoyama2，Yoshikazu Tahara3，Yutaro Hayashi3 1AIST 2FEI Co. Japan 3Kyorin Univ. School of Med.

2P155 Membrane structure of microsomal glutathione transferase 1 (MGST1) by electron crystallography Peter J Holm2，Priyaranjan Bhakat2，Caroline Jegerschold2，Nobuhiko Gyobu1，○Kaoru Mitsuoka1，Yoshinori Fujiyoshi3，Ralf Morgenstern2，Hans Hebert2 1Japan Biological Information Research Center 2Karolinska Institutet 3Kyoto University

2P156 Solid-State NMR Measurement of H+-ATP Synthase Subunit c-ring Reconstituted into DMPC bilayers ○Kentaro Tanaka1，Masatoshi Kobayashi1，Takayuki Nakano1，Toshiharu Suzuki2,3，Masasuke Yoshida2,3，Toshimichi Fujiwara1，Hideo Akutsu1 1Institute for Protein Research, Osaka University 2Chemical Resources Laboratory, Tokyo Institue of Technology 3ATP System Project Exploratory Research for Advanced Technology (ERATO)

2P157 Molecular dynamics simulation of c-ring of F-type ATP synthase in explicit membrane ○Kyoko Nakajima1，Sotaro Fuchigami1，Mitsunori Ikeguchi1，Akinori Kidera1 1Yokohama City Univ, Internat. Graduate School of Arts and Sciences, Supramolecular Biol.

2P158 Molecular Dynamics Simulations of Mechano-Gating in the E-coli Mechanosensitive Channel MscL ○Yasuyuki Sawada1，Masaki Murase2，Masahiro Sokabe1,2,3 1Dept. Physiol., Nagoya Univ. Grad. Sch. Med 2ICORP/SORST Cell Mechanosensing, JST 3Dept. Mol. Physiol., NIPS, NINS

2P159 Attachment of yellow fluorescent protein to influenza hemagglutinin at cytoplasmic tail inhibits membrane fusion activity of hemagglutinin

○Mari Gotoh1，Masae Takashima1，Tomoko Okada1，Yoshikatsu Ogawa1 1National Institute of Advanced Industrial Science and Technology (AIST)

2P160 The Importance of Single Residue in Retinal Binding Pocket for Color-tuning and Stability in Microbial Rhodopsins ○Keon Ah Lee1，Ah Reum Choi1，Sa Ryong Yoon1，Kwang-Hwan Jung1 1Dept. of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University

2P161 Reconstitution of bacteriorhodopsin into artificial cyclic phospholipid membrane ○Masashi Sonoyama1，Shigeki Mitaku1，Motonari Shibakami2，Shin Miyoshi2 1Dept. Appl. Phys., Nagoya Univ. 2AIST

2P162 Kinetic Study for Irreversible Photobleaching of Bacteriorhodopsin in Purple Membrane at Alkaline pH ○Yasunori Yokoyama1，Masashi Sonoyama1，Shigeki Mitaku1 1Department of Applied Physics, Graduate School of Engineering, Nagoya University

2P163 Effect of Ion Concentration on Regeneration of Bacteriorhodopsin from Bacterio-opsin and All- trans Retinal ○Naoki Koito1，Hiroyuki Sugiyama1，Masashi Sonoyama1，Shigeki Mitaku1 1Dept of Applied Physics, Nagoya Univ.

2P164 Live cell imaging of GLUT4 molecules in adipocytes using fluorescent quantum dot and TIRF microscopy ○Hideaki Fujita1，Tomonobu M. Watanabe1，Taku Nedachi1，Hideo Higuchi1，Makoto Kanzaki1 1TUBERO, Tohoku Univ.

2P165 Biophysical investigations on the interaction of the major bovine seminal plasma protein, PDC-109 with phospholipid membranes and heparin

○Musti J. Swamy1，V. Anbazhagan1，Rajani S. Damai1 1School of Chemistry, University of Hyderabad, Hyderabad - 500 046

2P166 A comparison of structural properties in N-terminal domain of connexin26 associated to KID syndrome ○Takahiro Tanji1，Yoshitaka Umetsu1，Masakatsu Kamiya2，Tomoyasu Aizawa1，Yasuhiro Kumaki1，Ken Arita3，Masashi Akiyama3，Hiroshi Shimizu3，Makoto Demura2，Keiichi Kawano1 1Grad. Sch. of Sci., Hokkaido Univ. 2Grad. Sch. of Life Sci., Hokkaido Univ. 3Grad. Sch. of Med., Hokkaido Univ.

2P167 Probing the bound conformation of Acetylcholinesterase (AChE) inhibitor at the binding site ○Xin Zhao1,2，Chang Gyeom Kim2，Scott Goodall2，Anthony Watts2 1Institute for Protein Research, University of Osaka 2Department of Biochemistry, University of Oxford

35. RNA world 2P168 Structural Basis of RNA-Dependent Recruitment of Glutamine to the Genetic Code

○Hiroyuki Oshikane1，Kelly Sheppard2，Yuko Nakamura1，Shuya Fukai1，Liang Feng2，Tomoyuki Numata1，Ryuichiro Ishitani1，Dieter Soll2，Osamu Nureki1 1Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology 2Department of MB&B, Yale University

2P169 Molecular basis for RNA acetylation to maintain the decoding fidelity ○Sarin Chimnaronk1，Tateki Suzuki1，Tetsuhiro Manita1，Yoshiho Ikeuchi2，Min Yao1，Tsutomu Suzuki2，Isao Tanaka1 1Fac. of Adv. Life Sci., Hokkaido Univ., Sapporo 2Dept. of Chem. and Biotech., Grad. Schl. of Eng., Univ. of Tokyo, Tokyo

2P170 Protein biosynthesis by ribosomes immobilized on solid surfaces ○Toshihiko Matsuura1，Hiroyuki Tanaka2，Tomoji Kawai2 1Lab. of Biotechnology, Hokkaido Univ. of Education 2ISIR-SANKEN, Osaka Univ.

36. DNA to chromatin 2P171 Dependency of thermostability on loop sequence in DNA mini-hairpin molecule revealed by locally enhanced sampling method

○Satoshi Yamasaki1，Shugo Nakamura1，Tohru Terada2，Kentaro Shimizu1 1Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo 2Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, The University of Tokyo

2P172 Predicted Effects of Local Conformational Coupling and External Restraints on the Torsional Properties of Single DNA Molecules

○Atsushi Matsumoto1，Wilma K. Olson2 1JAPAN ATOMIC ENERGY AGENCY 2Dept. of Chemistry & Chemical Biology, Rutgers, the State University of New Jersey

2P173 Sequence-dependent DNA conformation and flexibility characterized by hydration pattern ○Yoshiteru Yonetani1，Hidetoshi Kono2,3，Nobuhiro Go3 1CREST, JST 2PRESTO, JST 3Computational Biology Group, Neutron Biology Research Center, Japan Atomic Energy Agency

2P174 Spectroscopic analysis of DNA duplex including T-HgII-T base pairs ○Shuji Oda1，Hiroshi Yamaguchi1，Akira Ono2，Chojiro Kojima3，Yoshinori Kondo1，Yoshiyuki Tanaka1 1Grad. Sch. Pharm. Sci., Tohoku Univ. 2Fac. Eng., Kanagawa Univ. 3Grad. Sch. Biol. Sci., NAIST

2P175 Interaction of Palmatine with Polymorphic DNA Conformations: Spectroscopic, Viscometric, Competitive Dialysis and Thermodynamic Studies

○Kakali Bhadra1，Motilal Maiti1，Gopinatha S. Kumar1 1Biophysical Chemistry Laboratory, Indian Institute of Chemical Biology,Kolkata

2P176 New filamentous ordered structure appeared by stretching of unfixed human chromosome ○Kensuke Ikeda1，Osamu Hoshi2，Tatsuo Ushiki2，Hisashi Haga1，Kazushige Kawabata1 1Divisions of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan 2Division of Microscopic Anatomy and bio-imaging, Niigata university Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan

2P177 Rational combination of stabilizing strategies to achieve synergistic stabilization of nucleic acid assembly ○Takuma Katayama1，Atsushi Maruyama2,3，Satoshi Obika4，Takeshi Imanishi4，Hidetaka Torigoe1 1Tokyo University of Science 2Kyushu University 3Japan Science and Technology Agency 4Osaka University

2P178 Influence of the size of counterions on DNA hybridization as studied by dielectric spectroscopy ○Daisuke Yamamoto1，Yoichi Katsumoto1，Shinji Omori1，Akio Yasuda1 1Life Science Laboratory, Materials Laboratories, Sony Corporation

2P179 Dielectric Dispersion of Short Single-strand DNA in Aqueous Solutions with and without Added Salt ○Yoichi Katsumoto1，Shinji Omori1，Daisuke Yamamoto1，Akio Yasuda1，Koji Asami2 1Life Science Laboratory, Materials Laboratories, Sony Corporation 2Institute for Chemical Research, Kyoto Univ.

2P180 Analysis of sequence-dependent conformation of DNA backbone torsion angles by molecular dynamics simulations ○Satoshi Fujii1,2，Hidetoshi Kono3,4，Shigeori Takenaka5，Nobuhiro Go3，Akinori Sarai1 1Dept. Biosci. Bioinfo., Kyushu Inst. of Tchnol. 2ATI 3Cent. Prom. Compt. Sci. Eng., JAEA 4PRESTO 5Dept. Materials Sci., Kyushu Inst. of Tchnol.

37. Molecular motor (II) 2P181 The load dependent kinetics of the Chara myosin motor domain

○Hiroaki Kojima1，Kohji Ito2，Dietmar J. Manstein3，Keiichi Yamamoto2，Kazuhiro Oiwa1 1Biological ICT group, NICT 2Dept. of Biology, Chiba Univ. 3Dept. of Biophys. Chem., Hannover Med. Sch.

2P182 Intramolecular Correlation and Structural Change of Myosin Motor Domain ○Fumiko Takagi1,2，Macoto Kikuchi1,2 1JST CREST 2Cybermedia Center, Osaka University

2P183 How Does the Energy Released by ATP Hydrolysis Spread throughout the Myosin Head?: Molecular Dynamics Simulations

○Tatsuyuki Kawakubo1，Okimasa Okada2，Tomoyuki Minami3 1Toin Univ. of Yokohama 2Fuji Xerox Co., Ltd. 3Fuji Photo Film Co., Ltd

2P184 Three dimensional nanometry of moving vesicles in a living cell ○Tomonobu M Watanabe1，Takashi Sato1，Kohsuke Gonda1，Hideo Higuchi1 1Tohoku University Biomedical Engineering Research Organization

2P185 The role of charged residues in C-terminus of PomA in V. alginolyticus ○Madoka Obara1，Toshiharu Yakushi2，Seiji Kojima1，Michio Homma1 1Division of Biological science, Graduate school of science, Nagoya University 2Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University

2P186 A reversibility parameter for Markovian steppers

○Takahiro Harada1，Naoko Nakagawa2 1Department of Human and Artificial Intelligent Systems, University of Fukui 2Department Mathematical Sciences, Ibaraki University

2P187 Localization of the stator of the Na+-driven flagellar motor dependent on Na+ ion ○Tomoyuki Wada1，Hajime Fukuoka1，Seiji Kojima1，Michio Homma1 1Div. Biol. Sci. Nagoya univ.

2P188 Inter-modular Communication among Multiple Nucleotide-binding/hydrolysis AAA+ Modules of Cytoplasmic Dynein ○Toshifumi Mogami1，Takahide Kon1，Kenji Imamula1，Reiko Ohkura1，Kazuo Sutoh1 1Dept. of Life Sciences, Tokyo Univ.

2P189 ATP-driven rotation of VoV1 from Thermus thermophilus ○Masahiro Nakano1，Masatada Tamakoshi2，Hiromi Imamura3，Masasuke Yoshida1，Ken Yokoyama4 1Tokyo Institute of Technology 2Tokyo University of Pharmacy and Life Science 3Osaka University 4Japan Science and Technology Agency

2P190 A novel expression system for dominant negative mutant actins ○Taro Q.P noguchi1,2，Hironori Ueno2，Keiko Hirose2，Taro Q.P Uyeda2 1Life and Environmental Sciences, Univ. of Tsukuba 2Research Institute for Cell Engineering, AIST

2P191 Mechanism of mechanical work and efficiency enhancement in myosin filament assembly ○Motoshi Kaya1，Hideo Higuchi1 1Biomedical Research Organization, Tohoku Univ

2P192 Dissection of Intra-molecular Communications between the Catalytic Head and Microtubule-binding Site in the Dynein Heavy Chain

○Takahide Kon1，Kenji Imamula1，Reiko Ohkura1，Kazuo Sutoh1 1Dept. of Life Sci., Grad. Sch. of Arts & Sci., Univ. of Tokyo

2P193 Characterization of interaction between β- and γ-subunit in F1-ATPase using twisted γ mutant ○Satoko Shibano1，Eiro Muneyuki2，Tomoko Masaike1，Kaoru Okada1，Masasuke Yoshida3，Takayuki Nishizaka1,4 1Gakushuin Univ., Fac. Sci., Dept. Phys. 2Chuo Univ. 3Tokyo Institute of Technology. 4PRESTO, JST

2P194 Angle Dependent Affinity of Rotary Motor F1-ATPase for Nucleotides ○Kengo Adachi1，Takayuki Nishizaka2，Hiroyuki Noji3，Hiroyasu Itoh4，Kazuhiro Oiwa5，Masasuke Yoshida6，Kazuhiko Kinosita, Jr.1 1Waseda Univ 2Gakushuin Univ 3Osaka Univ 4Hamamatsu Photonics & CREST 5Kansai Adv Res Ctr 6Tokyo Inst Tech & ERATO

2P195 Observation of the tug of war movement of microtubules by kinesin and dynein molecules in vitro ○Hideyo Tsurusawa1，Hisashi Tadakuma1，Tomohiro Shima2，Reiko Ohkura2，Takahide Kon2，Kazuo Sutoh2，Michio Tomishige1 1Dept. of Applied Physics, Faculty of Engineering, Univ. of Tokyo 2Dept. of Life Sciences, Graduate School of Arts and Sciences, Univ. of Tokyo

2P196 Coiled-coil domain of myosin V ○Takayuki Ariga1，Tomotaka Komori1，Atsuko_H. Iwane1，Toshio Yanagida1,2 1Graduate School of Frontier Biosciences, Osaka Univ. 2CREST, JST

2P197 The head of myosin class-V motor moves along an actin filament with regular, ~5.5 nm steps. ○Takuya Okada1,2，Hiroto Tanaka1,2，Atsuko_Hikikoshi Iwane2,3，Kazuo Kitamura4，Mitsuo Ikebe5，Toshio Yanagida1,2,3 1Laboratories for Nanobiology, Graduate School of Frontier Biosciences 2Formation of soft nano-machines, CREST JST

3Department of Physiology and Biosignaling, Graduate School of Medicine Osaka University 4Department of Neuroscience, Graduate School of Medicine Osaka University 5UMASS

2P198 High-resolution observations of the movement of single kinesin molecules using FIONA ○Keishi Kikuchi1，Shuichi Onishi1，Yuya Miyazono1，Hiroaki Fujiwara1，Hisashi Tadakuma1，Michio Tomishige1 1Dept. of Applied Physics ,Faculty of Engineering, Univ. of Tokyo

2P199 Conformational Changes of Inner-Arm Dynein f from Chlamydomonas Coupled with Phosphorylation of Its Intermediated Chain IC138. III

○Hitoshi Sakakibara1，Stanley A Burgess2，Yukako Sakai1 1Kobe Advanced Research Center, NICT 2 Institute of Molecular and Cellular Biology, University of Leeds

2P200 Simultaneous observation of myosin V and polyacrylamide bead along actin tracks using two colors TIRFM ○Yuta Saito1，Naoki Nagura1，Shin'ichiro Tanaka1，Toshio Ando1,2 1Dept. of Physics, Kanazawa Univ. 2JST/CREST

2P201 Interpretation of "Maxwell demon" in the induced potential motor molecule model from the viewpoint of the stochastic energetics

○Tetsuya Shimokawa1 1Graduate School of Engineering Science, Osaka University

2P202 Dynein movement on zinc-induced tubulin sheets Atsuko Nishimura1，○Yoko Y. Toyoshima1 1Departement of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo

2P203 Unit step-size in the movement of myosin filament ○Masatoshi Nishikawa1，Hiroaki Takagi1，So Nishikawa2，Toshio Yanagida1,2 1Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka Univ. 2Formation of Soft Nanomachines, CREST

2P204 Identification of minimal motor domain of cytoplasmic dynein ○Naoki Numata1，Takahide Kon1，Reiko Ohkura1，Kazuo Sutoh1 1Dept. of Life Sciences, Tokyo Univ.

2P205 Behavior of single molecules of cytoplasmic dynein on microtubules in vitro

○Michi Miura1，Aiko O. Matsubara1，Takuya Kobayashi1，Ken'ya Furuta1，Masaki Edamatsu1，Yoko Y. Toyoshima1 1Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo

2P206 The contribution of individual dynein head to the processive movement generated by two single headed dynein ○HoaAnh Nguyen1，Motoshi Kaya1，Takahide Kon2，Kazuo Sutoh2，Hideo Higuchi1 1Biomedical Engineering Research Organization, Tohoku University 2Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo

2P207 F1-ATPase with a Truncated Rotor ○Mohammad D. Hossain1,2,3，Shou Furuike1，Yasushi Maki4，Kengo Adachi1，Yusuf M. Ali2，Mominul Huq3，Kazuhiko Kinosita Jr1 1Dept. of Physics, Fac. of Sci. and Eng., Waseda Univ. 2Dept. of Physics, School of Physical Sci., Shahjalal Univ. of Sci. and Tech. 3Dept. of Physics, Fac. of Eng. , Bangladesh Univ. of Eng. and Tech. 4Dept. of Physics, Osaka Medical College

2P208 Three-dimensional structure of dynein elucidated by electron cryo-microscopy ○Takuo Yasunaga1，Ryo Saito1，Takahide KOn2，Reiko Ohkura2，Masaya NIshiura2，Kazuo Sutoh2 1Department of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology 2Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo

2P209 Analysis of conformational changes of functional loop L11 of kinesin using Fluorescence Probe and ESR ○Masafumi Yamada1，Toshiaki Arata2，Kazunori Kondo1，Hidekatsu Maeda1，Shinsaku Maruta1 1Dept. of Eng., Grad. Sch, of Soka Univ. 2Dept. of Biol., Grad. Sch, of Sci., Osaka Univ.

2P210 Kinetic Analysis of Dynein-MT Interaction ○Kenji Imamula1，Takahide Kon1，Reiko Ohkura1，Kazuo Sutoh1 1Dept. of Life Sci., Univ. of Tokyo

2P211 Mechanical Modulation of ATP binding affinity and ATP hydrolysis / synthesis equilibrium of F1-ATPase ○Daichi Okuno1，Rikiya Watanabe2，Shouichi Sakakihara1，Katsuya Shimabukuro3，Masasuke Yoshida4，Hiroyuki Noji1 1ISIR, Osaka Univ. 2Department of Mechanical Engineering, The University of Tokyo 3Florida State Univ. 4Chemical Resources Laboratory, Tokyo Tech.

2P212 Mapping the N-terminal region of FliI that is involved in the regulation of its ATPase activity ○Mayuko Okabe1，Tohru Minamino2,3，Keiichi Namba2,3，May_K Macnab1 1Dept. of MB&B, Yale University 2Dynamic NanoMachine Project, ICORP, JST 3Graduate school of Frontier Biosciences, Osaka University

2P213 Processive movement of minus end-directed microtubule motor protein Pkl1 ○Ken'ya Furuta1，Masaki Edamatsu1，Yoshinori Takahashi1，Yoko Y. Toyoshima1 1Graduate School of Arts & Sciences, The University of Tokyo

2P214 Effect of adenine nucleotide on dynein motility ○Hideaki Saegusa1，Ken'ya Furuta1，Masaki Edamatsu1，Yoko Y. Toyoshima1 1Department of Life Sciences, Graduate school of Arts And Sciences, The University of Tokyo

2P215 A model of reversible molecular machine for free energy transduction ○Eiro Muneyuki1，Ken Sekimoto2 1Faculty of Science and Engineering, Department of Physics, Chuo University 2MSC CNRS/UMR 7057, Université Paris

2P216 Nucleotide dependent intermolecular cross-linking of rice kinesin by photo reactive ATP analogue. ○Yutaka Koike1，Nobuhisa Umeki2，Toshiaki Mitsui3，Yasunobu Sugimoto4，Katsuzo Wakabayashi4，Shinsaku Maruta1 1Division of Bioengineering, Graduate School of Engineering, SOKA University, Hachioji, Tokyo, 192-8577 2Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0127 3Group of Plant and Microbial Genome Controls, Graduate School of Science and Technology, NIIGATA University, Niigata, Tokyo, 950-2181

4Division of Biophysical Engineering, Graduate School of Engineering Science, OSAKA University, Toyonaka, Osaka 560-8531

2P217 Cross-linking of kinesin using bifunctional photochromic compounds ○Yuki Nakajima1，Eichi Kobayashi2，Masafumi Yamada1，Masato Ito1，Shinsaku Maruta1 1Div.of Bioeng., Grad.Sch.of Eng., Soka Univ. 2Dept.of Bioinfo., Fac.of Eng., Soka Univ.

2P218 Characterization of a Novel Rice Kinesin D04 and Its Interaction with Fluorescent ATP analogue ○Yuko Kubo1，Nobuhisa Umeki2，Kazunori Kondo1，Toshiaki Mitsui3，Shinsaku Maruta1 1Dept. of bioinfo., Fac. of eng., Soka Univ. 2Dept. of Phys., Univ. of Mass. Med. Sch. 3Grad. Sch. of Sci. and Tech., Niigata Univ.

2P219 Interaction of a novel rice kinesin O12 with fluorescent ATP analogue ○Nozomi Umezu1，Yuko Kubo3，Nobuhisa Umeki4，Kondo Kazunori1,3，Toshiaki Mitsui2，Shinsaku Maruta1,3 1Div. of bioeng., Grad. Sch. of Eng., Soka Univ. 2Grad. Sch. of Scie. and Tech., Niigata Univ. 3Dept. of Bioinfo., Fac. of Eng., Soka Univ. 4Dep.of Phys., UMMS

2P220 Insigh into The molecular rotation of c-subunit ring in Thermophilic Bacillus PS3 ATP synthase ○Prihardi Kahar1，Toshiharu Suzuki1，Masasuke Yoshida1 1ATP System Project, ERATO of JST, Tokyo Institute of Technology

2P221 Energy landscape of actomyosin elucidating multi-step directional sliding ○Mitsunori Takano1 1Dept. of Physics, Waseda Univ.

2P222 Dynamic Behavior of Dynein C Captured by High-speed AFM ○Atsushi Miyagi1，Hitoshi Sakakibara2，Kazuhiro Oiwa2，Toshio Ando1,3 1Dept. of Physics, Kanazawa Univ. 2Kansai Advanced Research Center 3CREST, Japan Science and Technology Agency

2P223 Unidirectional Orientation of Actin Filaments ○Makito Miyazaki1，Katsuyuki Shiroguchi1，Kazuhiko Kinosita, Jr.1 1Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo

2P224 Analysis of the movement of the fluorescent dot composed of GFP-FliG with a flagellar basal body structure in the cell membrane

○Hajime Fukuoka1，Yoshiyuki Sowa2，Seiji Kojima1，Akihiko Ishijima3，Michio Homma1 1Div. of Biol. Sci., Grad. Sch. of Sci., Nagoya Univ. 2Clarendon Lab., Univ. of Oxford 3Inst. of Multidisciplinary Research for Advanced Materials, Tohoku Univ.

2P225 Torque and Potential measurement of F1-ATPase ○Tetsuaki Okamoto1，Akilan Palanisami1，Kazuhiko Kinosita Jr.1 1Department of Physics, Waseda University

2P226 Myosin V mutations uncouple ATPase activity from motility ○Takashi Ohki1，Naoki Mochizuki1 1Dept. of Structural Analysis, National Cardiovascular center research Institute

2P227 Analysis of single molecule motility of mitotic kinesins ○Satoshi Iwasaki1，Ken'ya Furuta1，Toshihiko Sakuma1，Masaki Edamatsu1，Yoko Y. Toyoshima1 1Dept. of Life Sciences, Tokyo Univ.

2P228 Photochemical Control of Loading/Unloading of Q-Dots onto Microtubules: Application of Host-Guest Chemistry to Micro-transporting System.

Kazuaki Kato1，○Kaoru Katoh1，Rie Goto1，Motonari Shibakami1 1Aist

2P229 Effect of arginine and its analogs for the Na+-driven flagellar motors of alkalophilic Bacillus. ○Tatsuo Atsumi1，Shigeru Sugiyama2 1Gifu University of Medical Science 2NFRI, National Agriculture and Food Research Organaization

2P230 Gene regulation for a specific inhibition of the typeIII secretion of Salmonella virulence factors by monoterpenes of plant essential oils

○Hideaki Mizusaki1,3，Yumiko Yamasaki2，Kazuya Akimitsu2，Aizawa Shin-Ichi1,3 1Department of Life Sciences, Prefectural University of Hiroshima 2Department of Life Sciences, Kagawa University

3CREST, Japan Science & Technology Agency (JST)

38. Chemo-mechanical coupling 2P231 Loose coupling between chemical reaction and mechanical work in kinesin

○Yuichi Taniguchi1，Masayoshi Nishiyama2，Yoshiharu Ishii3，Toshio Yanagida1,3 1Graduate School of Frontier Biosciences, Osaka University 2Department of Chemistry, Kyoto University 3Japan Science and Technology Agency

2P232 Coupling of Mechano- and ATP Signaling in Mammary Glands Revealed by Imaging of ATP Released from the Tissue ○Kishio Furuya1，Kumi Akita1，Masahiro Sokabe1,2 1Cell-mechanosensing, ICORP and SORST, JST 2Dept Physiol, Nagoya Univ Grad School Med

39. Cell motility 2P233 Studying a theoretical model explaining diverse morphologies of amoebae

○Shin I. Nishimura1，Masaki Sasai1 1Dept. of Comp. Sci. and Eng., Nagoya Univ.

2P234 Spontaneous cell motion in the developmental process of Dictyostelium discoideum ○Hiroaki Takagi1,2，Masayuki J. Sato1，Toshio Yanagida1，Masahiro Ueda1,2 1Graduate School of Frontier Biosciences, Osaka Univ. 2MEXT Leading Project (Bio Nano Process)

2P235 Roles of PI3 kinase in electrotacitc response of Dictyostelium cells ○Airi Takayama1，Masayuki Sato1，Thumkeo Dean1，Toshio Yanagida1，Masahiro Ueda1 1Laboratories for Nanobiology , Graduate School of Frontier Bioscience , Osaka Univ.

2P236 Directional reversal of electrotactic movements in cyclic GMP metabolic mutants ○Masayuki J. Sato1，Hidekazu Kuwayama2，Hiroaki Takagi1，Toshio Yanagida1，Masahiro Ueda1 1Graduate School of Frontier Biosciences, Osaka Univ. 2Graduate School of Life and Environmental Sciences, Tsukuba Univ.

2P237 Morphology of isolated MvspI, a putative antigenic variation protein of Mycoplasma mobile, the fastest gliding mycoplasma

Jun Adan-Kubo1，Hidetoshi Kono2,3，○Makoto Miyata1,3 1Osaka City University 2Japan Atomic Energy Agency 3PRESTO, JST

2P238 Isolation and characterization of P1 adhesin involved in gliding motility of Mycoplasma pneumoniae. ○Daisuke Nakane1，Jun Adan-Kubo1，Tsuyoshi Kenri2，Makoto Miyata1,3 1Department of Biology, Graduate School of Science, Osaka City University 2Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Musashimurayama 3PRESTO, JST, Sumiyoshi-ku, Osaka

2P239 Experimental analysis of the motion of mono-flagellated bacteria in aqueous solutions containing polymers Megumi Nishitoba1,2，Yukio Magariyama3，Masaaki Iino2，○Seishi Kudo1 1Toin Univ. of Yokohama 2Chiba Inst. of Tech. 3Nat. Food Res. Inst.

2P240 How does high pressure affect on the bacterial motility? ○Masayoshi Nishiyama1，Yoshiyuki Sowa2，Shigeichi Kumazaki1，Yoshifumi Kimura3，Michio Homma4，Akihiko Ishijima5，Masahide Terazima1 1Dept. of Chemistry, Kyoto Univ. 2Dept. of Physics, Oxford Univ. 3International Innoavtion Center, Kyoto Univ. 4Division of Biological Science, Nagoya Univ. 5Division of Biomolecular Science,Tohoku Univ.

2P241 Live imaging of growth cone regeneration after axotomy of dorsal root ganglion neurons ○Mami Nomura1，Tomomi Tani3，Yoshie Harada1,2 1Dept. of Fronteir Science, Tokyo Univ. 2The Tokyo Metropol. Inst. of Med. Sci. 3Research Inst. of Electronic Science, Hokkaido Univ.

2P242 Dynamics of the intranuclear structure observed with pupil projection apodized phase contrast microscopy

○Hiromi Miyoshi1,3，Tatsuro Otaki2，Kaoru Katoh1 1Neuroscience Research Institute, AIST 2Core Technology Center, Nikon Co. 3NEDO

2P243 Imaging of membrane protrusion of cellular migration in tumor using quantum dots ○Kohsuke Gonda1，Tomonobu M Watanabe1，Hideo Higuchi1 1Biomedical and Engineering Research Organization, Tohoku University

2P244 Membrane hyperpolarization induced membrane fluidization in Paramecium: Correlation with the regulation of ciliary beat frequency

○yasuo nakaoka1，junya uemura1 1osaka university

2P245 GGT (gamma-Glutamyltranspeptidase) enhances migration of preosteoclasts and thereby stimulates multinuclear osteoclast formation

○Keiko Suzuki1，Sawako Moriwaki2，Shumpei Niida2，Shoji Yamada1 1Dept of Pharmacol, Sch of Dent, Showa Univ 2Dept of Bone and Joint Disease, Natl Center for Geriatrics and Gerontol

2P246 Roles of Lasp-1 at focal adhesions of melanoma B16 cells Yutaka Morita1，Ming Yue Jin1，Asako Terasaki2，Hiroyuki Nakagawa3，○Shigeaki Miyamoto1 1Dept. Biochem. and Bioinformatics, Kyushu Inst. Technology 2Dept. Biology, Faculty of Science, Chiba Univ. 3Dept. Biology, Faculty of Science, Fukuoka Univ.

2P247 Molecular analysis of Driving forces of Actin retrograde flow ○Shouichi Ikeda1，Yosuke Senju1，Hidetake Miyata1 1Dept. of Physics, Tohoku Univ.

2P248 Pointed end structure of Actin Filament ○Akihiro Narita1,2，Toshiro Oda1,2，Yuichiro Maéda1,2,3 1JST ERATO MAEDA Actin-filament Dynamics Project 2Harima Riken 3Nagoya Univ.

2P249 Inhibition of Mycoplasma mobile gliding proteins by monoclonal antibodies isolated from phage display library of human scFv

○Shuhei Yoshii1，Makoto Miyata1,2 1Dept. of Biology, Graduate School of sci. Osaka City Univ. 2PRESTO, JST

2P250 Elevation of intracellular cAMP concentration at tips of filopodia alters actin organization in neuronal growth cones. ○Makoto Goda1，Ayumu Inutsuka2，Yoshinori Fujiyoshi2 1RIKEN Harima Institute 2Kyoto University., Science, Biophysics

2P251 The effects of cytokine concentration on the flow around a suspended chemotactic neutrophile in liquid ○Masaaki Tamagawa1，Yuta Kubomoto1，Kusuhiro Mukai2，Yoshinori Furukawa3 1Grad. Sch. of Life Sci. and Sys. Eng., Kyushu Inst. of Tech. 2Kurosaki Harima 3Institute of Low Temperature Science, Hokkaido University

2P252 Simultaneous Recording of Traction Force and Molecular Dynamics in a Migrating Dictyostelium Cell ○Yoshiaki Iwadate1，Shigehiko Yumura1 1Faculty of Sci., Yamaguchi Univ.

2P253 Effects of stretch and BTS on auto-oscillatory properties of contraction and Ca2+ concentration in cultured cardiac muscle

○Mutsuki Takeuchi1，Shin'ichi Ishiwata2 1Grad Sch Pure/Appl Phys, Waseda Univ 2Dept Phys, Sch Sci/Eng, Waseda Univ

2P254 Strain Field Measurement of Cytoskeletal Actin Network in Lamellipodia of Crawling Fish Keratocytes ○Taiji Adachi1,2，Kennedy O. Okeyo1，Yoshimichi Shitagawa1，Masaki Hojo1 1Dept. of Mech. Eng. & Sci., Kyoto Univ. 2VCAD System Res. Prog., RIKEN

2P255 Influences of the hook length on the rotational vibration of a tethered cell ○Manami Hashimoto1，Takuji Mashimo1，Shin-Ichi Aizawa1,2 1CREST JST 2Pref. Univ.of Hiroshima

2P256 Physicochemical and structural analysis of mutant FlhA, a subunit protein of the flagellar type III protein export apparatus of Salmonella

○Masafumi Shimada1,2，Yumiko Saijyo-Hamano2，Yukio Furukawa2，Thoru Minamino1,2，Katsumi Imada1,2，Kei-ichi Namba1,2 1Graduate School of Frontier Biosciences, Osaka University 2Dynamic NanoMachine Project, ICORP, JST

2P257 Characterization and crystallization of the flagellar rod cap protein FlgJ from Salmonella ○Yuki Kikuchi1，Hideyuki Matsunami2，Midori Yamane1，Katsumi Imada1,2，Keiichi Namba1,2 1Graduate School of Frontier Biosciences, Osaka University 2Dynamic NanoMachine Project, ICORP, JST

2P258 Purification and crystallization of the FliM-FliN complex from Salmonella Roland Degenkolbe1，○Shigehiro Nagashima2，Fadel Samatey1,2，Hideyuki Matsunami1,2，Katsumi Imada1,2，Keiichi Namba1,2 1Grad. Sch'l of Frontier Biosci., Osaka Univ. 2Dynamic Nanomachine Project, ICORP, JST

2P259 X-ray diffraction from dynein motors and microtubules in sea-urchin sperm flagellar axonemes. ○Shinji Kamimura1，Hiroyuki Iwamoto2，Tetsuro Fujisawa3 1Dept. Life Sci., Grad. Sch. Arts & Sci., Univ. Tokyo 2Life & Env. Div. Spring-8, JASRI 3Struct. Biol. Lab., Harima Inst., Instit. Phys. & Chem. Res.

2P260 Structure analysis of the flagellar hook-basal body with C ring by electron cryomicroscopy ○Tomoko Miyata1，Takayuki Kato1，Takashi fuji1，Keiichi Namba1,2 1Graduate school of Frontier Biosciences, Osaka University 22. Dynamic NanoMachine Project, ICORP, JST

2P261 Analysis of the conformation of intracellular flagellin ○Yukio Furukawa1，Hideyuki Matsunami1，Samatey_A Fadel1，Tohru Minamino2，Keiichi Namba1,2 1JST. ICORP. 2Grad. of Frontier Biosciences, Osaka Univ.

2P262 Molecular structure and dynamics of cytoplasmic domain of FlhA, a subunit of the flagellar type III protein export apparatus

○Yumiko Saijo-Hamano1，Katsumi Imada1,2，Tohru Minamino1,2，May Kihara3，Akio Kitao4,5，Keiichi Namba1,2 1Dynamic NanoMachine Project, ICORP, JST 2Graduate School of Frontier Biosciences, Osaka Univ. 3Department of Molecular Biophysics and Biochemistry, Yale Univ. 4Institute of Molecular and Cellular Biosciences, Univ. of Tokyo

5CREST, JST 2P263 Structural analysis of the FliI ring, the ATPase component of the flagellar type III protein export apparatus, by electron cryomicroscopy

○Ken-ichi Kazetani1,2，Tohru Minamino1,2，Mayuko Okabe3，Takayuki Kato1，Keiichi Namba1,2 1Graduate school of Frontier Biosciences, Osaka University 2Dynamic NanoMachine Project, ICORP, JST 3Department of Molecular Biophysics and Biochemistry, Yale University

2P264 Molecular characterization of a flagellar periplasmic protein, FlgA ○Hideyuki Matsunami1，Yukio Furukawa1，Keiichi Namba1,2 1Dynamic NanoMachine, ICORP, JST 2Grad. Sch. Front. Biosci., Osaka Univ.

2P265 Structure of the Bacterial Flagellar Poly-rod by Electron Cryomicroscopy and Image Analysis ○Takashi Fujii1,2，Takayuki Kato1，Tomoko Miyata1，Fabienne Chevance3，Kelly Hughes3，Keiichi Namba1,2 1Graduate School of Frontier Biosciences, Osaka University 2Dynamic NanoMachine Project, ICORP, JST 3Department of Biology, University of Utah

2P266 Analysis of the flagellar rod in flgG mutants ○Noriko Takahashi1，Fabienne Chevance3，Kelly T Hughes3，Shin-Ichi Aizawa1,2 1CREST JST 2Pref. Univ. of Hiroshima 3Univ. of Utah

2P267 Correlation between the flagellar hook length and FliK molecular size ○Satoshi Shibata1,2，Noriko Takahashi1，Favienne F. V. Chevance3，Kelly T. Hughes3，Shin-Ichi Aizawa1,2 1CREST JST 2Pref. Univ. of Hiroshima 3Univ. of Utah

2P268 Temperature sensitive sites in the rotor components of flagellar motor. ○Takuji Mashimo1，Manami Hashimoto1，Shigeru Yamaguchi2，Shin-Ichi aizawa3 1CREST JST 2Univ. of Gunma 3Pref. Univ. of Hiroshima

2P269 Structural study of FlhB, a membrane component of the flagellar type III protein export apparatus ○Fadel A. Samatey1,2，Yumiko Saijo-Hamano1,2，Tohru Minamino1,2，Jonathan McMurry3，Keiichi Namba1,2 1Dynamic NanoMachine, ICORP, JST 2Graduate School of Frontier Biosciences, Osaka University 3Department of Molecular Biophysics and Biochemistry, Yale University

40. Membrane structure 2P270 Database of Lipid Membrane Structures: Computational Analyses of Model Membranes

○Kenichi Mori1，Yosuke Imai1，Tsubasa Takaoka1，Koji Iwamoto1，Hideyoshi Fuji1，Tyuji Hoshino1 1Grad. Sch. of Pharm. Chiba University

2P271 Structural investigation on highly asymmetric sphingomyelin (C24:0 SM) bilayers ○Hiroshi Takahashi1，Tomohiro Hayakawa2，Yuko Kawasahi3，Kazuki Ito4，Tetsuro Fujisawa4，Michiko Kodama3，Toshihide Kobayashi2 1Dept. of Physics, Gunma Univ. 2Lipid Biology Lab., RIKEN 3Det of Biochem., Okayama Univ. of Sci. 4RIKEN Harima Institute/SPring-8

2P272 Effect of Cholesterol on Condensation of Phosphatidylinositol and Sphingomyelin Mixed Monolayer ○Masanao Kinoshita1，Satoru Kato1 1Department of Physics, Advanced Research Center of Science in Kwansei-Gakuin Univ.

2P273 Efficient molecular recognition by lectin to the glyco-functionalized self-assembled monolayers ○Kyoko Yoshioka1，Yukari Sato1，Teiichi Murakami1，Osamu Niwa1 1National Institute of Advanced Industrial Science and Technology (AIST)

2P274 Atomic force microscopic observation of ganglioside GM3-containing membrane microdomain Kazutoshi Iijima1，Teruhiko Matsubara1，○Toshiori Sato1 1Keio University

2P275 Surface Force Measurements of Interactions between Glycolipids Monolayers ○Daisuke Fukushi1，Hiroshi Sakuma1，Kazue Kurihara1 1Institute of Multidisciplinary Research for Advanced Materials

2P276 Specific interactions between the carbohydrate on gold nanoparticles and lectins ○Yukari Sato1，Teiichi Murakami1，Kyoko Yoshioka1，Osamu Niwa1 1National Institute of Advanced Industrial Science and Technology (AIST)

2P277 Promiscuous ligand binding of β-lactoglobulin allowed by hydrophobic interactions and structural variability ○Tsuyoshi Konuma1，Kazumasa Sakurai1，Yuji Goto1,2 1Institute for Protein Research, Osaka University 2CREST/JST

2P278 Giant Liposomes in Micro-Fluidic Device -Towards an efficient preparation, manipulation and immobilization method-

○Kaori Kuribayashi1，Bruno Le Pioufle2， Shoji Takeuchi1 1CIRMM-IIS, The University of Tokyo 2LIMMS/CNRS-IIS, The University of Tokyo

2P279 Formation of Ultra Giant Vesicles from a Planar Lipid Memebane ○Kei Funakoshi1，Hiroaki Suzuki1，Shoji Takeuchi1 1Institute of Industrial Science, The University of TOKYO

2P280 Asymmetric fusion between a giant unilamellar vesicle and large unilamellar vesicles applied to controlling a cell-sized microreactor

○Kanta Tsumoto1，Makoto Inaba1，Tetsuro Yoshimura1 1Dept. of Chem. for Materials, Grad. Sch. of Eng., Mie Univ.

2P281 Investigation of pH-induced membrane-fusion properties of baculovirus by microscopic observation of individual giant unilamellar vesicles

○Koki Kamiya1，Kanta Tsumoto1，Tetsuro Yoshimura1 1Department of Chemistry for Materials Graduate School of Engineering Mie University

2P282 Study on the Packing Structure of the Stratum Corneum Lipid Model by Electron Diffraction ○Isao Takagishi1，Akira Yamagishi1，Hiromitsu Nakazawa1，Shingo Sakai2，Shintaro Inoue2，Satoru Kato1 1Dept. of Science and Technology, Kwansei-Gakuin Univ. 2Kanebo Cosmetic Inc.

2P283 Study on the Lipid Domain of Mammalian Stratum Corneum by Electron Diffraction. ○Akira Yamagishi1，Isao Takagishi1，Tomohiro Imai1，Hiromitsu Nakazawa1，Shingo Sakai2，Shintarou Inoue2，Satoru Kato1 1School of Science&Technology, Kwanseigakuin Univ. 2Kanebo Cosmetics Inc.

2P284 Effect of cations on stratum corneum lipid monolayer ○Takashi Kojima1，Masanao Kinoshita1，Hiromitsu Nakazawa1，Satoru Kato1 1School of Science and Technology, Kwansei Gakuin University

2P285 Mechanism of liposome leakage induced by fatty acid divalent cations ○Ayumu Kato1，Machiko Iwata1，Nobuyuki Wakayama1 1Dept. of Biomed. Engin., Toin Univ. of Yokohama

2P286 Structure and Dynamics of Raft Model Membrane ○Mitsuhiro Hirai1，Teruaki Onai1，Harutaka Hirai1，Gang Chen 1 1Dept. of Physics, Gunma Univ. 2Dept. of Physics, Gunma Univ. 3Dept. of Physics, Gunma Univ. 4Dept. of Physics, Gunma Univ.

2P287 Spontaneous Formation of a Solid-Supported Mixed Bilayer Composed of Phospholipid and Nonionic Surfactant under an Aqueous Medium

○Ryo Ishiguro1，Michiko Nakao1，Tomohiro Sugiura1，Buemho Byn1，Keiichi Kameyama1，Koichi Hiramatsu1 1Fac. of Eng., Gifu Univ.

2P288 Supported phospholipid bilayers on SiO2 and TiO2 surfaces: Effects of surface chemical species and atomic structures ○Ryugo Tero1，Hidekazu Watanabe2，Toru Ujihara3，Tsuneo Urisu1 1Instit. for Molecular Sci. 2RIKEN 3Nagoya Univ.

2P289 Composite Membranes of Fluid and Partially Polymerized Phospholipid Bilayers ○Takashi Okazaki1,2，Kenichi Morigaki2，Koji Asami3，Takahisa Taguchi2 1Osaka Univ. 2AIST 3ICR Kyoto Univ.

2P290 Kinetics of subgel formation in DPPC/DOPC mixed bilayers ○Keisuke Ito1，Masanao Kinoshita1，Satoru Kato1 1Advanced Research Center of Science, School of Science and Technology, Kwansei Gakuin University

2P291 Construction of higher-ordered monolayer membranes derived from cyclic lipids that are inspired by archaeal membrane lipids

○Makoto Nakamura1，Rie Goto1，Toshio Tadokoro1,2，Motonari Shibakami1 1National Institute of Advanced Industrial Science and Technology (AIST) 2Chiba Institute of Technology

2P292 Construction of supported planar membranes derived from cyclic lipids ○Motonari Shibakami1，Rie Goto1，Makoto Nakamura1 1AIST

2P293 Formation of domains in fluorescent lipids doped DMPC-DOPC binary bilayers supported on SiO2/Si substrates under local light irradiation.

○Shoya Suzuki1，Toru Ujihara1，Ryugo Tero2，Yoshikazu Takeda1 1Department of Crystalline Materials Science, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan 2Department of Vacuum UV Photoscience, Institute for Molecular Science, Myodaiji, Okazaki 444-8585, Japan

2P294 The interaction of melittin with PC membranes and PE membranes investigated with respect to lipid packing effect ○Atsuji Kodama1，Tetsuhiko Ohba1，Kazuo Ohki1 1Dept. of Physics, Tohoku Univ.

2P295 Phase diagram of DMPC/melittin systems ○Kouya Tamatsukuri1，Tetsuhiko Ohba1，Kazuo Ohki1 1Dept. of Physics. Tohoku Univ.

2P296 Cancel 2P297 Favorable neutral-, anionic lipids compositions for the binding of hormone peptide to lipid bilayer as revealed by solid state NMR and QCM

○Katsuyuki Nishimura1，Hiroki Myoga2，Aya Harada2，Eisuke Takeda2，Atsushi Kira3，Akira Naito2 1Institute for Molecular Science 2Yokohama National University 3ULVAC, Inc

2P298 Construction of Langmuir-Blodgett film of dimyristoyl-phosphatidylcoline and bacteriorhodopsin layered on fatty acid film.

○Yukinobu Makino1，Manabu Ito1，Yutaka Tsujiuchi1 1Department of Material Science and Engineering, Akita University

2P299 Langmuir- Brodgett film of retinoic acid and synthetic AKV peptides of bacteriorhodopsin on hydrogenated amorphous silicon.

○Yutaka Tsujiuchi1，Yukinobu Makino1，Hiroshi Masumoto2，Takashi Goto2

1Department of Material Science and Engineering, Akita University 2Institute of Materials Research, Tohoku University 2P300 Biochemical analyses combined with single molecule mechanics reveal the nuclear membrane targeting mechanism to chromatin

○Yasuhiro Hirano1，Hirohide Takahashi1，Tsuneyoshi Horigome2，Shige H. Yoshimura1，Kunio Takeyasu1 1Grad. Sch. of Biostudies, Kyoto Univ. 2Grad. Sch. of Sci. and Tech., Niigata Univ.

2P301 Imaging Caveolae-like vesicle complexs in Cultured Visceral Adipocyte with Electron Microscopy ○Junji Sekimoto1，Masafumi Nagayama1，Tsutomu Uchida1，Kyoko Shimizu2，Toshio Taira2，Kazutoshi Gohara1 1Dept. of Engineering, Hokkaido Univ 2Primary Cell Co.,Ltd.,

2P302 Partition Coefficient of a Local Anesthetic into Model Biomembranes with Cholesterol-induced Domain Structure ○Masato Tamura1，Manabu Hasebe1，Kazuhiko Matsuoka2，Kazuhito Nagayama1，Takashi Hata1，Hiromu Satake3，Hitoshi Matsuki4，Shoji Kaneshina4 1Department of Material Science and Technology, Kochi National College of Technology 2Advanced Course in Materials Science and Engineering, Kochi National College of Technology 3Center for Cooperative Research, The University of Tokushima 4Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima

2P303 Study of the interaction between lidocaine and lipid membranes with respect to effect on phase-separated micro domains

○Shintaro Yamashina1，Kazuo Ohki1 1Department of Physics, Tohoku University

2P304 High binding affinity of the amino group in the peptide for the neutral phospholipid membrane ○Yumi Yoshida1，Yuta Nakagawa1，Kohji Maeda1 1Dept. of Chemistry, Kyoto Institute of Technology.

41. Proton and ion pumping 2P305 Probing proton channel in cytochrome bd-type ubiquinol oxidase by site-directed mutagenesis

○Tatsushi Mogi1, 2，Hideto Miyoshi3 1Chem. Resources Lab., Tokyo Inst. Technol., 2ERATO, JST, 3Div. Appl. Life Sci., Grad. Sch. Agriculture, Kyoto Univ.

2P306 H-D unexchangeable N-H group of Trp182 in Bacteriorhodopsin ○Maiko Yoshitsugu1，Mikihiro Shibata1，Hideki Kandori1 1Department of Materials Science and Engineering, Nagoya Institute of Technology

2P307 Theoretical study on the conformational change from theneutral form to the acid blue form of bacteriorhodopsin ○Hiroshi Watanabe1，Masanori Tsuzuki1，Takahisa Yamato1,2 1Graduate School of Science, Nagoya University 2CREST, JST

2P308 Asp to Glu substitution in the cytoplasmic channel dramatically affects the photocycle of bacteriorhodopsin-like protein from a Eucaryote

○Yuji Furutani1,2，Masayo Sumii1，Ying Fan3，Lichi Shi3，Stephen A. Waschuk3，Leonid S. Brown3，Hideki Kandori1,2 1Dept. of Mat. Sci. & Eng., Nagoya Inst. of Tech. 2CREST, JST 3Dept. of Phys., Univ. of Guelph

2P309 Biophysical properties and putative function of the two microbial rhodopsins in Acetabularia acetabulum ○Yuna YUN1，Ah reum CHOI1，Kwang-Hwan JUNG1 1Dept. of Life science and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul

2P310 Spectroscopic and Biochemical Characterization of Proteorhodopsin in Maxwell Bay, Antarctica ○Jae Yong JUNG1，Yuna YUN1，Keon Ah LEE1，Kwang-Hwan JUNG1 1Dept. of Life science and Interdisciplinary Program of integrated Biotechnology, Sogang Univ. Seoul, Korea

2P311 The role of E234 on the extracellular site in the anion pump of pharaonis halorhodopsin ○Yuko Saito1，Takanori Sasaki1，Megumi Kubo1，Takashi Kikukawa3，Akiteru Seki1，Seiji Miyauchi1，Tomoyasu Aizawa2，Keiichi Kawano2，Naoki Kamo1，Makoto Demura1 1Grad. Sch. Life Sci., Hokkaido Univ. 2Grad. Sch. Sci., Hokkaido Univ. 3CRIS, Hokkaido Univ.

2P312 The comprehensive analysis of the color tuning residues of pharaonis halorhodopsin by site-saturation mutagenesis ○Masatoshi Matsunami1，Fumie Tatami1，Megumi Kubo2，Tomoyasu Aizawa1，Keiichi Kawano1，Makoto Demura2 1Grad. Shc. Sci., Hokkaido Univ. 2Grad. Shc., Life Sci, Hokkaido Univ.

2P313 Structural network in the extracellular region corresponding to the visual spectral shift of halorhodopsin ○Megumi Kubo1，Takanori Sasaki1，Yuko Saito1，Takashi Kikukawa2，Naoki Kamo1，Tomoyasu Aizawa3，Keiichi Kawano3，Makoto Demura1 1Div. Life Sci., Grad. School Life Sci., Hokkaido Univ. 2CRIS, Hokkaido Univ. 3Dept. Biol. Sci., Grad. School Sci., Hokkaido Univ.

2P314 Transient movement of helices of halorhodopsin during the photocycle. ○Mina Habara1，Megumi Kubo1，Tomoyasu Aizawa2，Keiichi Kawano2，Makoto Demura1 1Div. of Life Sci., Grad. School of Life Sci., Hokkaido Univ. 2Dept. of Biol. Sci., Grad. School of Sci., Hokkaido Univ.

2P315 Trimer-monomer conversion of pharaonis halorhodopsin by anion binding ○Marika Higuchi1，Takanori Sasaki1，Tomoyasu Aizawa2，Keiichi Kawano2，Makoto Demura1 1Grad. Sch. Life Sci., Hokkaido Univ. 2Grad. Sch. Sci., Hokkaido Univ.

2P316 The change of trimer packing intensity of halorhodopsin caused by binding or release of Cl- ion ○Takanori Sasaki1，Tomoyasu Aizawa2，Masakatsu Kamiya1，Keiichi Kawano2，Makoto Demura1 1Grad. Sch. Life Sci., Hokkaido Univ. 2Grad. Sch. Sci., Hokkaido Univ.

42. Sensory signal transduction 2P317 In situ calcium imaging of ATP-sensitive perigemmal cells in mouse

○Mitsuo Akutsu1，Keita Takeuchi2，Kiyonori Yoshii2，Takashi Kumazawa1 1Dept. of Mater. Sci. and Eng., Saitama Inst. Tech. 2Grad. Sch. Life Sci. and Eng., Kyushu Inst. Tech.

2P318 Origin of vertebrate photoreceptor: ON-type and OFF-type photoreceptors in ascidian larva

○Yoshiaki Toda1，Takeo Horie1，Takehiro Kusakabe1，Hiroko Horiguchi2，Takahiko Hariyama2，Motoyuki Tsuda1 1Department of Life Science, Graduate School of Life Science, University of Hyogo 2Department of Biology, Faculty of Medicine, Hamamatsu University School of Medicine

2P319 CNG channels in the ascidian Ciona intestinalis: Insights into the origin and evolution of vertebrate CNG channels ○Kaori KAMATA1，Takehiro KUSAKABE1，Mariko KOTERA1，Masashi NAKAGAWA1，Yuki MIYAMOTO1，Motoyuki TSUDA1 1Graduate School of Life Science, University of Hyogo

2P320 Cancel 2P321 Distinct features of the bidirectional and unidirectional flagellar motors of marine Vibrio

○Masaru Kojima1，Igor B Zhulin3，Michio Homma1，Ikuro Kawagishi1,2 1Div. of Bio. Sci., Grad. Sch. of Sci., Nagoya Univ. 2Inst. for Adv. Research, Nagoya Univ. 3Joint Inst. for Comp. Sci., The Univ. of Tennessee - Oak Ridge Nat. Lab.

2P322 Effect of culture temperature on chemotaxis of Vibrio cholerae. ○Daisuke Suzuki1，Akihiro Hyakutake1，Michio Homma1，Ikuro Kawagishi1,2 1Div. of Biol. sci., Grad. School of sci. Nagoya Univ. 2Inst. for Advanced Reserch, Nagoya Univ.

2P323 Structural determinants of the ligand specificities of the bacterial amino acid chemoreceptors ○Hirotaka Tajima1，Katsumi Imada2，Mayuko Sakuma1，Fumiyuki Hattori1，Midori Sumii3，Toshifumi Nara3，Naoki kamo3，Michio Homma1，Ikuro Kawagishi1,4 1Div. of Biol. Sci., Grad. Sch. of Sci. Nagoya Univ. 2Grad. Sch. of Front. Biosci., Osaka Univ. 3Grad. Sch. of Pharm. Sci., Hokkaido Univ 4Inst. for Adv. Res., Nagoya Univ.

2P324 Attractant binding to one type of chemoreceptor affects a different type of chemoreceptor within a mixed cluster in Escherichia coli

○Hiroki Irieda1，Michio Homma1，Ikuro Kawagishi1,2 1Div. of Biol. Sci., Grad. Sch. of Sci., Nagoya Univ. 2Inst. for Adv. Research, Nagoya Univ.

2P325 Co-localization of the redox sensor Aer with the chemoreceptors of Escherichia coli ○Noriko Ohta1，Satomi Banno1，Michio Homma1，Ikuro Kawagishi1,2 1Div. of Biol. sci., Grad. School of sci. Nagoya Univ. 2Inst. for Advanced Reserch, Nagoya Univ.

2P326 Direct observation of the movement of the bacterial chemoreceptor through the cytoplasmic membrane ○Satomi Banno1，Michio Homma1，Ikuro Kawagishi1,2 1Div. of Biological Science, Grad. Sch. of Science, Nagoya Univ. 2Inst. for Advanced Research, Nagoya Univ.

2P327 Structural study of pHtrII linker region by NMR and EPR ○Kokoro Hayashi1，Yuki Sudo2，Masaki Mishima1，Hideyuki Hara3，Naoki Kamo2，Chojiro Kojima1 1Graduate School of Biological Science, Nara Institute of Science and Technology 2Graduate School of Pharmaceutical Sciences, Hokkaido University 3Department of Bruker BIO SPIN

2P328 Effect of azide on dynamics of light-induced conformational change of phoborhodopsin/transducer complex ○Yukinori Taniguchi1，Tatsuya Ikehara1，Naoki Kamo2，Hiroshi Yamasaki1，Yoshinori Toyoshima1 1Nano-biotechnology research center,and Department of Bioscience, School of Science & Technology, Kwansei Gakuin Univ. 2Graduate School of Pharmaceutical Science, Hokkaido Univ.

2P329 Molecular Dynamics Study of Sensory Rhodopsin II Complex with Cognate Transducer ○Koro Nishikata1，Masanori Hashido1，Sotaro Fuchigami1，Mitsunori Ikeguchi1，Akinori Kidera1 1Supramolecular Biology, Yokohama City University

2P330 Photochromism of Anabaena sensory rhodopsin ○Akira Kawanabe1，Yuji Furutani1,2，Kwang-Hwan Jung3，Hideki Kandori1,2 1Department of Materials Science and Engineering, Nagoya Institute of Technology 2CREST/JST 3Sogang University

2P331 The activation mechanism of rhodopsin as studied by MD simulation for a constitutively active mutant E113Q/M257Y

○Yuto Morita1，Yuuki Miyagawa1，Minoru Sakurai1 1Center for Biol. Res. Info., Tokyo Tech

2P332 Interaction of Arrestin with Phosphorylated Rhodopsin C-terminus Peptide ○Yohei Ikeda1，Junzo Gohara1，Yasushi Imamoto1，Hironari Kamikubo1，Yoichi Yamazaki1，Toru Kawakami2，Shoko Yoshimura2，Saburo Aimoto2，Mikio Kataoka1 1Graduate School of Materials and Science, Nara Institute of Science and Technology 2Institute for Protein Research, Osaka University

2P333 Elucidation of Interaction Domains for AppA-PpsR Complex ○Seiji Ano1，Yoichi Yamazaki1，Hironari Kamikubo1，Yasushi Imamoto1，Mikio Kataoka1 1Graduate School of Material Science, Nara Institutes of Science and Technology

2P334 Light-induced dissociation of SyPixD (cyanobacterial BLUF type photoreceptor) from SyPixE (response regulator) ○Koji Okajima1，Shusei Sato2，Yoshimasa Fukushima3，Akiko Kita4,5,6，Yuriko Ochiai7，Mitsunori Katayama7，Shigeru Itoh3，Satoshi Tabata2，Masahiko Ikeuchi7 1Dept. of Biol. Science Osaka Prefec. Univ. 2Kazusa DNA Research Institute 3Grad. School of Science Nagoya Univ. 4Research Reactor Institute Kyoto Univ. 5Dept. of Chemistry Kyoto Univ. 6RIKEN Spring-8 7Dept of Life Sciences Tokyo Univ.

2P335 Molecular dynamics study on the structure of the FAD binding site of some BLUF proteins ○Kazuya Obanayama1，Minoru Sakurai1 1Center for Biol. Res. Info., Tokyo Inst. Tech

2P336 Molecular dynamics simulations of the Gln1029Leu mutant of phy3 LOV2 domain in the dark and S390 states ○Hiroaki Kobayashi1，Mika Nabeno1，Minoru Sakurai1 1Center for Biol. Res. Info., Tokyo Inst. Tech.

2P337 Computational study on the photochemical reactivities of Adiantum Phy3-LOV1 and LOV2. Role of Phe1010 located

near FMN in LOV2. ○Mika Nabeno1，Minoru Sakurai2 1Mitsubishi Pharma Corporation 2Center for Biol. Res. Info., Tokyo Inst. Tech.

2P338 Energy transfer pathways in photosensory protein ○Takakazu Ishikura1，Hiroshi Watanabe1，Takahisa Yamato1,2 1 Graduate School of Science, Nagoya Univ. 2CREST, JST

2P339 Neutron diffraction and high resolution X-ray crystal structure analysis of photoactive yellow protein ○Shigeo YAMAGUCHI1，Hironari KAMIKUBO1，Nobutaka SHIMIZU2，Yoichi YAMAZAKI1，Yasushi IMAMOTO1，Mikio KATAOKA1 1Graduate School of Materials Science, Nara Institute of Science and Technology 2SPring-8/JASRI

2P340 Conformational Change of β5-β6 Loop during the Photocycle of Photoactive Yellow Protein ○Yasushi Imamoto1，Hiroaki Sasayama1，Miki Harigai1，Yoichi Yamazaki1，Hironari Kamikubo1，Mikio Kataoka1 1Graduate School of Materials Science, Nara Institute of Science and Thechnology

2P341 Correlation between Photocycle Kinetics of PYP and Conformation of β4-β5 Loop ○Miki Harigai1，Yoichi Yamazaki1，Yasushi Imamoto1，Mikio Kataoka1 1Graduate School of Materials and Science, Nara Institute of Science and Technology

2P342 The Photocycle of Met100Ala Mutant of Photoactive Yellow Protein Studied by Low-Temperature Spectroscopy ○Takashi Morimoto1，Miki Harigai1，Yasushi Imamoto1，Mikio Kataoka1 1Graduate School of Materials Science, Nara Institute of Science and Technology

2P343 Isolation of Photoactive Yellow Protein associated protein from Rhodobacter capsulatus ○Yoichi Yamazaki1，Hironari Kamikubo1，Yasushi Imamoto1，Mikio Kataoka1 1Graduate School of Materials Science, Nara Institute of Science and Technology

43. Photosynthesis 2P344 Roles of calcium ions on the structural stability of the LH1-RC core complex from thermophilic purple bacterium Thermochromatium tepidum

○yukihiro Kimura1，Hiroaki Suzuki1，Dao rina1，Masao Nakamura1，Seiu Otomo1 1Dept. of Molecular Chemistry and Biology

2P345 Solution Structure of the Rhodobacter sphaeroides PufX Membrane Protein ○Seiu Otomo1，Hiroaki Suzuki1 1Faculty of Science, Ibaraki Univ.

2P346 Femtosecond Fluorescence Up-Conversion Study of Light Harvesting and Electron Transfer in Intact Purple Photosynthetic Bacterial Membrane

○Yutaka Shibata1，Yosuke Satoh1，Daiju Fujita1，Tetsuo Tomii1，Shigeru Itoh1 1Dept. of Physics, Nagoya Univ.

2P347 Photosynthetic energy and electron transfer in the reaction center complex of Acidiphilium rubrum containing Zn- bacteriochlorophyll a

○Tetsuo Tomi1，Yutaka Shibata1，Yuki Ikeda1，Seiji Taniguchi2，Chosrowjan Haik2，Noboru Mataga2，Keizo Shimada3，Shigeru Itoh1 1Dept. of Material Science (Physics), Nagoya Univ. 2Institute for Laser Technology 3Dept. of Biology, Tokyo Metropolitan Univ.

2P348 Properties of iron-sulfur centers and quinone in the reaction center complex of Heliobacterium modesticaldum ○Hirozo Oh-oka1，Ryo Miyamoto2，Hiroyuki Mino2，Shigeru Itoh2 1Dept. Biol., Grad. Sch. Sci., Osaka Univ. 2Div. Mat. Sci., Grad. Sch. Sci., Nagoya Univ.

2P349 Fast electron donation from CycA to the homodimeric type-I reaction center of the green sulfur bacterium Chlorobium tepidum.

○Chihiro Azai1，Yusuke Tsukatani2，Shigeru Itoh3，Hirozo Oh-oka1 1Dept. of Biol. Sci., Grad. Schl. of Sci., Osaka Univ. 2Inst. for Biol. Resour. and Func., Natl. Inst. of AIST. 3Div. of Matl. Sci. (Phys.), Grad. Schl. of Sci., Nagoya Univ.

2P350 Identification of electron transfer proteins reducing photochemical reaction center of the filamentous anoxygenic phototrophic bacteria

○Yusuke Tsukatani1，Nahomi Nakayama2，Katsumi Matsuura2，Keizo Shimada2，Satoshi Hanada1，Kenji V.P. Nagashima2 1National Institute of Advanced Industrial Science and Technology 2Dept. of Biology, Tokyo Metropolitan Univ.

2P351 Energy coupling, dephasing and thermalization in two electron-transfer reactions ○Keisuke Saito1，Koichiro Mukai1，Hitoshi Sumi1 1Institute of Materials Science, Univ. of Tsukuba

2P352 Origin of the rapid fluctuation of the electron tunneling matrix element for the long-range electron transfer in protein environment

○Hirotaka Nishioka1，Toshiaki Kakitani1 1Dept. of General Education, Faculty of Science and Technology, Meijo Univ.

2P353 Delayed fluorescence from charge separated states mediated by the excited special pair in the reaction center of bacterial photosynthesis

○Koichiro Mukai1，Keisuke Saito1，Hitoshi Sumi1 1Inst. of Materials Science, Tsukuba Univ.

2P354 Theoretical analysis of the electronic asymmetry of the special pair in the photosynthetic reaction center: Effect of protein environment

○Hideki Yamasaki1，Yu Takano1，Haruki Nakamura1 1IPR, Osaka Univ

2P355 Theoritical Analysis of Proton Transfer in the Photosynthetic Reaction Center from Rohodobacter sphaeroides ○Yu Kaneko1，Shigehiko Hayashi2，Shinji Saito3，Iwao Ohmine1

1Dept. of Chemistry, Nagoya Univ. 2Dept. of Chemistry,Kyoto Univ. 3Insitute for Molecular Science 2P356 The tautomerism in the oxygen-evolving complex of photosystem II as revealed by the G=2 and G=4 EPR signals in the S2-State

○Masami Kusunoki1 1Dep. of Physics, Meiji Univ.

2P357 Proton Release Pattern during Photosynthetic Water Oxidation as Studied by Flash-Induced FTIR Difference Spectroscopy

○Hiroyuki Suzuki1，Miwa Sugiura2，Takumi Noguchi1 1Inst. of Mater. Sci., Univ. of Tsukuba 2Dept. of Plant Biosci., Osaka Pref. Univ.

2P358 Effects of P680 ligand substitution in the oxygen-evolving Photosystem II of Thermosynechococcus elongatus ○Miwa Sugiura1，Alain Boussac2，Takumi Noguchi3，Fabrice Rappaport4 1Dep. of Plant Biosciences, Osaka Prefecuture University 2Service de Bioénergétique, CEA Saclay 3Inst. of Mater. Sci., Univ. of Tsukuba 4Institut de Biologie Physico-Chimique

2P359 Hydrogen bonding structure of the redox-active tyrosine YD in photosystem II as studied by FTIR spectroscopy and DFT calculations

○Ryouta Takahashi1，Hiroyuki Suzuki1，Miwa Sugiura2，Alain Boussac3，Takumi Noguchi1 1Inst. of Mater. Sci., Univ. of Tsukuba 2Dept. of Plant Biosci., Osaka Pref. Univ 3CNRS, CEA Saclay

2P360 Requirement of Phosphatidylglycerol for the assembly and function of photosystem I and II reaction centers ○Tatsuya Uzumaki1，Josef Komenda2，Masayuki Komura1，Bettina Ughy3，Ildikó Domonkos3，Anna Sallai3，Yoshimasa Fukushima1，Balázs Bogos3，Hajime Wada4，Zoltán Gombos3，Shigeru Itoh1 1Grad. Sch. of Sci., Nagoya univ. 2Inst. of Microbiol., Opatovicky mlyn 3Inst. of Biochem., Biol. Res. Cent. of Hungarian Acad. of Sci. 4Dept. of Life Sci., Univ. of Tokyo

2P361 The chlorophylls replacement in the photosynthesis ○Min Chen1，Zhengli Cai1 1University of Sydney

2P362 Photosystems of Acaryochloris marina and Gloeobacter violaceus ○Shunsuke Ohashi1，Sojiro Fukuyo1，Yoshihiro Shiraiwa 2，Tohru Tsutiya 3,4，Hideaki Miyashita 3,4，Mamoru Mimuro 3,4，Masami Kobayashi 1 1Institute of Materials Science, University of Tsukuba 2Institute of Biological Sciences University of Tsukuba 3Graduate School of Human and Environmental Studies, Kyoto University 4Department of Technology and Ecology, Hall of Global Environmental Research, Kyoto University

2P363 Function of photosynthetic membrane protein complex inside silica mesoporous materials ○Souji Ishizaka1，Ippei Oda1，Tsutomu Kajino2，Yoshiaki Fukushima2，Takeshi Sekitoh3，Shigeru Itoh1 1Division of Material Science, Graduate School of Science, Nagoya University 2 Toyota Central R&D Labs. Inc. 3Material Engineering Div.3 TOYOTA MOTOR CORPORATION

2P364 Photoprotection Mechanism in Dried Lichens Studied by Pico-Second Time-Resolved Fluorescence Spectrophotometry at Cryogenic Temperature

○Masayuki Komura1，Ikuko Iwasaki2，Shigeru Itoh1 1Division of Mateiral Science (Physics), Graduate School of Science, Nagoya Univ. 2Dept. of Bio-Resource Science, Akita Prefectural Univ.

44. Neuro-biophysics 2P365 Gene Expression of Serotonin Transporter in Learning Animals

○Hisayo Sadamoto1，Etsuro Ito1 1Facl. Pharm. Sci., Kagawa Campus, Tokushima Bunri Univ.

2P366 Characteristics of synaptic connection during development in Lymnaea stagnalis. ○Yuko Abe1，Manabu Sakakibara1，Tetsuro Horikoshi1 1Tokai University graduate school

2P367 Photoresponse in statocyst hair cells of Lymnaea stagnalis ○Takeshi Karasawa1，Tetsuro Horikoshi2，Manabu Sakakibara2 1Laboratory of Neurobiological Engineering, Graduate School of High-Technology for Human Welfare, Tokai University

2Laboratory of Neurobiological Engineering, School of High-Technology for Human Welfare, Tokai University 2P368 Acetylcholine-induced calcium-NO signaling in the cricket terminal abdominal ganglion

○Hiroto Ogawa1，Yoshiichiro Kitamura2，Hitoshi Aonuma3，Kotaro Oka4 1Dept. of Biol., Fac. of Med., Saitama Med. Univ. 2Fac. of Pharmaceut. Sci. at Kagawa Campus, Tokushima Bunri Univ. 3Neuro-Cybern. Lab., Res. Inst. for Electronic Science, Hokkaido Univ. 4Dept. of Biosciences and Informatics, Fac. of Sci. and Tech., Keio Univ.

2P369 Microtomographic analysis of Drosophila brain Ryuta Mizutani1，○Tomohiro Hara1，Akihisa Takeuchi2，Kentaro Uesugi2，Yoshio Suzuki2 1Department of Applied Biochemistry, Tokai University 2Life & Environmental Div., JASRI / SPring-8

2P370 Analysis of vocal communication between mail and female zebra finches. ○Kotaro Oka1，Akira Fujimura1，Kohji Hotta1，Hiroto Ogawa2 1Dept. of Biosci. & Bioinfor., Keio Univ. 2Dept. Biol., Saitama Med. Univ.

2P371 A novel modeling on the cerebrovascular microcirculation to elucidate cerebral functional signal. ○Toru Yamada1，Shinji Umeyama1 1Neuroscience Research Institute, National Institute of advanced Industrial Science and Technology(AIST)

2P372 Ca2+ clearance mechanisms in presynaptic terminals : Ca2+ uptake to mitochondria occurred when [Ca2+]i increased to a critical level.

○Naoya Suzuki1，Toshihide Iiboshi1 1Dept. of Physics, Sch. of Science, Nagoya Univ.

2P373 Stimulation-induced Accumulation of internal Cd2+ reduced Transmitter Release and Short-term synaptic Plasticity at the Frog NMJ.

○Toshihide Iiboshi1，Naoya Suzuki1 1Dept. Phys. Sch. Sci. Nagoya Univ.

2P374 Ciliary motility change induced by neurotransmitters on rat brain ependymal cells. ○Kazuhiro Nakajima1，Makoto Goda2，Yoshinori Fujiyoshi1 1Department of Biophysics, Kyoto University Graduate School of Science 2RIKEN Harima Institute

2P375 Bursting input induced facilitation of signal transduction at theta band oscillatory signal transduction in hippocampus area CA1

○Takashi Tominaga1,2，Yoko Tominaga1，Michinori Ichikawa2 1Dept. Neurophys., Fac. Pharm. Sci. at Kagawa, Tokushima Bunri Univ. 2RIKEN BSI

2P376 Common cortical network associated with spontaneous changes of visual consciousness ○Tsutomu Murata1，Takashi Takayama1,2，Qiang Wei1，Tetsuto Minami1，Toshio Yanagida1,2，Ryouji Suzuki1,3 1National Institute of Information and Communications Technology 2Osaka University 3Kanazawa Institute of Technology

2P377 Electrophysiological and morphological classification of layer 1 neocortical somatosensory neurons ○Yumiko Ueno1，Brandi Mattson2，Henry Markram2，Tania Rinaldi2 1Dept. of Quantum Engineering and Systems Science, Tokyo Univ. 2Brain Mind Institute, Ecole Polytechnique Federale de Lausanne

2P378 RNG105 in neuronal RNA granules: Involvement in local translation and synapse formation ○Nobuyuki Shiina1,2，Kazumi Shinkura1，Makio Tokunaga1,2,3 1Structural Biology Center, National Institute of Genetics 2The Graduated University for Advanced Studies 3RCAI, RIKEN

2P379 Plasticity in single-cell-based reconstructed neuronal network pattern ○Ikurou Suzuki1，Akihiro Hattori2，Yasuhiko Jimbo3，Kenji Yasuda2 1Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo 2Department of Biomedical Information, Division of Biosystems, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University

3Department of Human and Engineered Environmental Studies, Graduate School of Frontier Sciences, University of Tokyo 2P380 On-chip regulation of neural stem cell differentiation

○Katsuya Shibata1，Ikurou Suzuki1，Hideyuki Terazono2，Yoshihiro Sugio1，Yasuda Kenji1,2 1Dept Life Sci, Grad Art&Sci, Univ Tokyo. 2Dept Biomed, Tokyo Medical&Dental Univ.

2P381 Controlling neurite differenciation by single cell neurite's elongation pattern and length using agarose micro chamber method.

○Tetsuo Kitamura1，Ikurou Suzuki1，Tomoyuki Kaneko1，Kenji Yasuda1 1Department of Life Sciences. Graduate Scool of Arts And Sciences, The University of Tokyo

2P382 Analysis of acute corticosterone effects on NMDA receptor-mediated Ca2+ signals in mouse hippocampal slices ○Hiromi Osanai1，Yoshimasa Komatsuzaki1，Suguru Kawato2，Minoru Saito1 1Grad. Sch. of Int. Sci., Nihon Univ. 2Grad. Sch. of Arts and Sci., Tokyo Univ.

2P383 Neurosteroids rapidly modulate the activity of hippocampal neurons at CA1 region. ○Yuuki Ooishi1，Suguru Kawato2,3 1Dept. of Physics. Tokyo Univ. 2Dept. of Arts and Sciences. Tokyo Univ. 3CREST. JST.

2P384 Non-genomic rapid effects of estradiol, a xenoestogen and androgen on hippocampal synapses ○Noritaka Inoue1，Mari Ogiue-Ikeda1，Norio Takada1，Kohei Nakajima1，Suguru Kawato1 1Dept. of Life Sciences, Graduate School of Arts and Sciences, Univ. of Tokyo, MEXT project

2P385 The distribution of estrogen receptor in the hippocampus ○Gen Murakami1，Shirou Kominami3，Hideo Mukai1,2，Tetuya Kimoto1，Suguru Kawato1,2 1The University of Tokyo 2CREST 3Hiroshima University

2P386 Synaptic Localization of Estrogen Receptor α in the Rat Hippocampus ○Hideo Mukai1,2，Shiro Kominami3，Gen Murakami1,2，William_GM Janssen4，John_H Morrison4，Suguru Kawato1,2 1Dept. of Biophysics & Life Sciences, Grad. Sch. Arts & Sciences, The University of Tokyo 2CREST, Bioinformatics Project, JST 3Grad. Sch. Integrated Arts & Sciences, Hiroshima University 4Dept. of Neuroscience, Mount Sinai School of Medicine

2P387 Synthesis of brain steroids and localization of P450s in the hippocampal synapses. ○Yasushi Hojo1,2,6，Kouhei Nakajima1,2，Hironori Nakanishi1,2，Shimpei Higo1,2，Hirotaka Ishii1,2，Hideo Mukai1,2，John H. Morrison3，William G. M. Janssen3，Shiro Kominami4，Nobuhiro Harada5，Tetsuya Kimoto1,2,6，Suguru Kawato1,2,6 1Dept. of Biophys and Life Sci., Grad. Sch. of Arts and Sci., The Univ. of Tokyo at Komaba 2CREST, Bioinformatics Project, JST 3Kastor Neurobiol. of Aging Lab., Fishberg Res. Cent. for Neurobiol., Mt. Sinai Sch. of Med. 4Fac. of Integrated Arts and Sci., Hiroshima Univ. 5Dept. of Biochem., Sch of Med., Fujita Health Univ. 6MEXT

2P388 Acute morphological effects of sex steroids and endocrine disruptors in hippocampal dendritic spines ○Yusuke Hatanaka1，Tomokazu Tsurugizawa1，Nobuaki Tanabe1，Suguru Kawato1 1Department of Biophysics and Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba

2P389 Immunohistochemical localization of steroidogenic enzymes in the rat hippocampus ○Miwa Fujiwara1，Gen Murakami1，hideo Mukai1，Suguru Kawato1 1Department of Life Sciences, Graduate School of Arts And Sciences, The University of Tokyo

2P390 Rapid morphological changes of dendritic spines mediated by glucocorticoid receptor (GR) in rat hippocampus ○Yoshimasa Komatsuzaki1,2,3，Gen Murakami2,3，Hironori Nakanishi2,3，Yasushi Hojo2,3，Suguru Kawato2,3 1Coll. of Hum. & Sci., Nihon Univ. 2Grad. Sch. of Arts & Sci., Univ. of Tokyo 3CREST, Bioinfomatics Project, JST

2P391 Androgen and estrogen synthesis in the rat hippocampus ○Shimpei Higo1，Yasushi Hojo1,2，Hirotaka Ishii1，Suguru Kawato1,3 1Dept. Biophys. and Life Sci, Grad. Sch. Art and Sci, Tokyo Univ. 2CREST, JST 3Bioinformatics, JST

2P392 Two modes of respiratory rhythm generation in the newborn rat brainstem-spinal cord preparation in vitro ○Hiroshi Onimaru1 1Dept. of Physiol., Showa Univ. School of Med.

45. Electronic structure 2P393 Theoretical study on color-tuning mechanism of visual pigments

○Kazuhiro Fujimoto1，Shigehiko Hayashi2，Jun-ya Hasegawa1，Hiroshi Nakatuji1 1Dept. of Synthetic Chemistry and Biological Chemistry, Kyoto Univ. 2Dept. of Chemistry, Kyoto Univ.

2P394 On the Yellow-Green Bioluminescence in North American Firefly : SAC-CI Study ○Naoki Nakatani1，Jun-ya Hasegawa1，Hiroshi Nakatsuji1 1Dept. of Syn. Chem. & Biol. Chem., Kyoto Univ.

2P395 The important role of AMP-Mg in promoting the decomposition of dioxetanone interacted with some amino acid residues in firefly luciferase

○Hironori Sakai1，Naohisa Wada1，Masami Kusunoki2 1Dept. of Life Sciences, Toyo Univ. 2Dept. of Physics, Meiji Univ.

2P396 Quantum mechanical study of the proton transfer via a peptide bond in the novel pathway of proton translocation of cytochrome c oxidase

○Yu Takano1，Haruki Nakamura1 1Institute for Protein Research, Osaka University

2P397 Magnetic Characterization of Daphnia resting eggs ○Masanobu Sakata1，Tamami Kawasaki2，Toshimichi Shibue3，Susumu Tsuruta4，Hideyuki Yoshimura4，Hideo Namiki1 1Dept. of Integrative Bioscience and Biomedical Engineering, Waseda Univ. 2ARISE, Waseda Univ. 3MCCL, Waseda Univ. 4Dept. of Physics, Meiji Univ.

2P398 A multicanonical ab initio molecular dynamics method: application to conformation sampling of alanine tripeptide ○Ryota Jono1，Tohru Terada1，Kentaro Shimizu1 1Graduate School of Agricultural and Life Sciences, The University of Tokyo

2P399 Solvation and interaction energy decomposition analysis of proteins from thefirst principles Dmitri G Fedorov1，Kazuo Kitaura1，○Yuto Komeiji1 1RICS/AIST

46. Water and bio-molecules 2P400 Electronic Polarization Fluctuation of Hydration Water in Biomolecular Solution

○Sachinori Ohta1，Hiroki Matsumoto1，Yasutaka Seki1，Kunitsugu Soda1 1Dept. of Bioeng., Nagaoka Univ. Technol.

2P401 Information of water structure by fluorescent probes ○George Mogami1，Takashi Miyazaki1，Makoto Suzuki1 1Dept. of Materials Processing, Tohoku Univ.

2P402 Structural and Thermodynamic Analysis of Hydrophobic Hydration by Molecular Dynamics Simulation ○Yudai Shimbo1，Yasutaka Seki1，Hiroki Matsumoto1，Kunitsugu Soda1 1Dept. of Bioeng., Nagaoka Univ. Technol.

2P403 Analysis method of classifying hydration shells of solutes by microwave dielectric spectroscopy ○Takashi MIYAZAKI1，Makoto SUZUKI1 1Graduate school of engneering, Tohoku university

2P404 Steric Conformity in Xe-Adsorption on a Protein and the Role of Solvent Molecules Hokuto Obama1，○Yasuhito Karino1，Ayako Yoshifuku1，Ryo Akiyama1 1Dept. of Chemistry, Graduate School of Science, Kyushu Univ.

2P405 Molecular dynamics simulation study on the interaction between trehalose and unsaturated bonds in aqueous medium

○Atsutoshi Okabe1，Minoru Sakurai1 1Center for Biol. Res. Info., Tokyo Inst. Tech

2P406 Molecular Dynamics Simulation on the Glassy States of Trehalose and Neotrehalose ○Naoko Kawasaki1，Takao Furuki1，Minoru Sakurai1 1Center for Biol. Res. Info., Tokyo Tech.

2P407 Molecular Dynamics Study on the interaction between trehalose and a lipid bilayer system Yuka Takano1，Naoko Kawasaki1，Takao Furuki1，○Minoru Sakurai1 1Center for Biol. Res. Info, Tokyo Inst. Tech.

2P408 Formation mechanism of Hyper-mobile water in alkali-halide solutions ○Makoto Nishida1，Takashi Miyazaki1，Makoto Suzuki1 1Dept. of Material processing,Tohoku Univ.

2P409 Microwave dielectric relaxation analysis of the property of hyper mobile water formation of sulfonic groups of AMPS ○Norihiko Tanno1 1dept. of material processing,Tohoku Univ.

2P410 Mechanical property of poly(vinyl-alcohol)-chondroitin-4-sulfate gel having hyper-mobile water ○Shizuka Saito1，Takashi Miyazaki1，Makoto Suzuki2 1Dept. of materials processing, Tohoku Univ. 2Dept. of materials processing, Tohoku Univ. msuzuki@tohoku.material.ac.jp

2P411 Diffusion coefficients of proton in polymer gels with hyper mobile water ○Tsubasa Ogawa1，Takashi Miyazaki1，Makoto Suzuki1 1Dept. of Material processing, Tohoku Univ

2P412 Hydration structures inside cyclodextrins: 3D-RISM analyses ○Yutaka Maruyama1，Yasuhiro Ikuta1，Fumio Hirata1

1Dept. of Theoretical Studies, IMS 2P413 Theoretical Study on Thermodynamic Stability of Micelles

○Tatsuhiko Miyata1，Fumio Hirata1 1Department of Theoretical Studies, Institute for Molecular Science

2P414 The Integral Equation Studies on Interaction between Charged Colloidal Particles Kohei Kaneda1，○Ryo Akiyama1，Masahiro Kinoshita2 1Department of Chemistry, Graduate School of Science, Kyushu University 2International Innovation Center, Kyoto University

2P415 Probing for hydrophobic molecules in cavities and clefts of a protein by the 3D-RISM theory ○Ryusuke Hiraoka1，Takashi Imai1，Andriy Kovalenko2,3，Tomoyoshi Seto5，Fumio Hirata4 1Department of Bioscience and Bioinformatics, Rrtsumeikan University 2National Institute for Nanotechnology, National Research Council of Canada 3Department of Mechanical Engineering, University of Alberta 4Department of Theoretical Studies, Institute for Molecular Science, 5Department of Anesthesilogy, Shiga University of Medical Science

2P416 Role of Collapse of Hydrophobic Cluster Residues in Protein Folding ○Hiroki Matsumoto1，Ismail E. Rozi2，Yasutaka Seki1，Kunitsugu Soda1 1Dept. of Bioeng., Nagaoka Univ. of Technol. 2Eijkman Inst. Molec. Biol.

2P417 Predictiction of hydrogen-bonding sites between Protein and Ligand by Molecular Dynamics Simulation ○Katsumi Murata1，Naoya Nagata1，Isao Nakanishi1，Kazuo Kitaura1 1Graduate School of Pharmaceutical Sciences, Kyoto University

2P418 Hydrophilic and hydrophobic effects on the function of an allosteric protein in solution ○Antonio Tsuneshige1，Takehiko Haga2 1Hosei Univ., Dept. of Frontier Bioscience, School of Engineering 2Hosei Univ., Dept. of Materials Chem., School of Engineering

2P419 Calorimetric titration study of F-actin solution with potassium chloride ○Hideyuki Komatsu1，Ying Gan Xin2，Takashi Miyazaki2，Akinori Sarai1，Makoto Suzuki2 1Dept of Bioinformatics and Bioscience, Kyushu Inst. of Tech. 2Dept of Materials Processing, Graduate School of Engineering, Tohoku Univ.

2P420 Two-colored emission from an intermediate M420 of firefly luciferin in deoxygenated DMSO under some content rates of water and t-BuOK

○Rikuo Shibata1，Yasuhiko Yoshida1，Naohisa Wada2 1Department of Applied Chemistry, Faculty of Engineering, Toyo University 2Department of Life Science, Faculty of Life Science, Toyo University

2P421 Cellular States of Dried Polypedirum Vanderplanki as Studied by Temperature-Controlled Fourier Transform Infrared Spectroscopy

○Takao Furuki1，Minoru Sakurai1，Ken-ichi Akao2，Masahiko Watanabe3，Takahiro Kikawada3，Takashi Okuda3 1Center of Biological resources and Informatics, Tokyo Institute of Technology 2JASCO Corporation 3National Institute of Agrobiological Science

2P422 Sequence Context Dependent Flexibility of DNA Studied by Molecular Dynamics Simulation Satoshi Fujii1，○Hidetoshi Kono2,3，Shigenori Takenaka1，Nobuhiro Go2，Akinori Sarai1 1Dept. Bioscience and Bioinformatics,Kyushu Institute of Technology 2Computational Biology, Japan Atomic Energy Agency 3PRESTO, JST

2P423 Competition Between Protein Ligands and Cytoplasmic Inorganic Anions for the Metal Cation ○Carmay Lim1,2，Todor Dudev1 1Institute of Biomedical Sciences, Academia Sinica 2Chemistry Department, National Tsing Hua University

47. Origin and evolution of life 2P424 Prebiotic oligomerization of amino acids under the non-equilibrium hydrothermal environments

○Eiichi Imai1，Hajime Honda1 1Dept. of BioEngineering, Nagaoka Univ. of Tech.

2P425 Adaptation dynamics for high temperature and heat tolerant evolution of Escherichia coli ○Masato Okubo1，Akiko Kashiwagi2，Toshihiko Kishimoto1，Tetsuya Yomo2,3,4 1Dept. of Biomolecular Science, Faculty of Science, Toho Univ. 2Graduate School of Information Science and Technology, Osaka Univ. 3Graduate School of Frontier Bioscience, Osaka Univ. 4Kaneko Complex Systems Biology Project, ERATO, JST

2P426 Phenotypic Noise, Error Catastrophe, and Genetic Evolution ○Kunihiko Kaneko1,2 1Department of Basic Science, Universuty of Tokyo 2ERATO Complex Systems Biology

2P427 Simulating evolution and development in animal body plan ○Fujimoto Koichi1,2，Shuji Ishihara3，Kunihiko Kaneko1,2 1Graduate School of Arts and Sciences, Univ. of Tokyo, 2ERATO Complex Systems Biology 3National institute for Basic biology

2P428 Mind Time and Space as a Dynamical System ○Kazuto Sasai1，Yukio Gunji1,2 1Graduate school of Science and Technology, Kobe Univesity 2Department of Earth and Planetary Science, Faculty of Science, Kobe University

2P429 The game of mandates : network structure of knowledge and decision making ○Asaki Nishikawa1 1Graduate School of Science and Technology, and, Department of Earth and Planetary Science,Faculty of Science, Kobe University

2P430 Structural change of a dynamical system derived from mixture of two limits

○Moto Kamiura1，Yukio P. Gunji1,2 1Graduate School of Science and Technology, KOBE University 2Department of Earth and Planetary Sciences, Faculty of Science, KOBE University

2P431 Motion perception and Perceptual filling-in at the blind spot ○Shimpei Tatsumi1，Yukio P Gunji1 1Dept.of Sci&Tech, Kobe Univ.

48. Bioinformatics, genomics and proteomics (II) 2P432 A novel statistical prediction method for multiple protein-protein interaction interfaces

○Wataru Nemoto1,2,4，Hiroyuki Toh3 1 Grad School of Infor Sci, NAIST 2BIC, ICR, Kyoto Univ 3MiB, Kyushu Univ 4Biochemistry Department, University of Cambridge

2P433 Modeling the emergence of cliques in the protein interaction networks ○Takeshi Hase1，Yoshihito Niimura1，Soichi Ogishima1，Tuguchika Kaminuma1,2，Hiroshi Tanaka1 1Dept. of Bioinformatics, Tokyo Medical and Dental Univ. 2Chem-Bio Informatics Society

2P434 Emergence of protein-protein interfaces: Insight from amino acid substitutions. ○Hafumi Nishi1，Motonori Ota2 1Grad. Sch. of Biosci. & Biotech., Tokyo Inst. of Tech. 2GSIC, Tokyo Inst. of Tech.

2P435 Exploring the minimal pathway maps ○Yusuke Azuma1，Motonori Ota1 1Tokyo Institute of Technology

2P436 Feature finding of cis regulatory elements by mutual entropy measurement ○Mutsuko Nishihama1，Wataru Matsubayashi1，Satoru Miyazaki1 1Department of Pharmaceutical Science, Tokyo University of Science

2P437 An improved thermodynamical model of hybridization on high-density oligonucleotide microarrays. ○Naoaki ONO1，Shingo SUZUKI2，Akiko KASHIWAGI2，Chikara FURUSAWA1,2，Tetsuya YOMO1,2,3 1ERATO, JST 2Dept. of Bioinfo. Eng., Osaka Univ. 3Grad. School of Frontier Biosciences, Osaka Univ.

2P438 Strategy to evaluate the effect of individual E. coli protein on the protein translation machinery ○Jiro Adachi1，Yasuaki Kazuta1，Tomoaki Matsuura2，Itaru Urabe2，Tetsuya Yomo1,3 1Dept. of Bioinformatics Engineering, Osaka Univ. 2Dept. of Biotechnology, Osaka Univ. 3ERATO, JST

2P439 Proteomic analysis of yeast ribosomal protein S10 mutant ○Youn Jin Park1，Yongbhum Song1，Won-Ki Huh1 1School of Biological Sciences, Research Center for Functional Cellulomics, and Institute of Microbiology, Seoul National University, Seoul 151-747, Korea

2P440 Identification of the proteins involved in production of magnetosome in Magnetospirillum magnetotacticum MS-1 ○Tsubasa Kuroki1，Watanabe Shingo1，Sawada Ken1，Iwasa Tatsuo1 1Dept. of Mat. Sci. and Eng., Muroran IT

2P441 Use of Proteomics and Cytomics to Analyze the Cell-cycle Regulation of Model Organisms ○Wei-Chen Liao1 1Department of Natural Science Education and Institute of Life Science

2P442 RefDIC: Construction of an open-access database of the quantitative transcriptome and proteome data of immune cells

○Atsushi Hijikata1，Hiroshi Kitamura1，Yayoi Kimura1，Ryo Yokoyama1，RCAI RefDIC supporting group1，Osamu Ohara1,2 1Research Center for Allergy and Immunology 2Kazusa DNA Research Institute

2P443 Renal cell carcinoma proteomics by agarose two-dimensional gel electrophoresis and LC-MS/MS ○Yoshihito Nii1，Hiroshi Okusa2，Kazumasa Matsumoto2，Masatsugu Iwamura2，Shiro Baba2，Masamichi Oh-Ishi1，Yoshio Kodera1,3，Tadakazu Maeda1 1Laboratory of Biomolecular Dynamics, Department of Physics, Kitasato University School of Science 2Department of Urology, Kitasato UniversitySchool of Medicine 3Clinical Proteomics Research Center, Chiba University Hospital.

2P444 Disease Peptidomics ○Yusuke Kawashima1，Yoshio Kodera1,2,3，Toshiyuki Fukutomi1，Hiroki Takahashi1，Tomoyuki Fukuno1，Takashi Matsui1，Sen-Ichi Furudate4，Akira Omori5，Takeshi Tomonaga3,6，Fumio Nomura3,6，Tadakazu Maeda1,2 1Laboratory of Biomolecular Dynamics, Department of Physics, School of Science, Kitasato University 2Center for Disease Proteomics, School of Science, Kitasato University 3Clinical Proteomics Research Center, Chiba University Hospital 4Department of Laboratory Animal Science, School of Medicine, Kitasato University 5Mitsubishi Kagaku Institute of Life Science (MITILS) 6Department of Molecular Diagnosis (F8), Graduate School of Medicine, Chiba University

2P445 High-molecular-mass proteome analysis of prostate cancer by agarose 2-DE and LC-MS/MS ○Masamichi Oh-Ishi1，Hiroshi Okusa2，Yoshihito Nii1，Kazumasa Matsumoto2，Masatsugu Iwamura2，Hidetoshi Kuruma3，Yoshio Kodera1，Tadakazu Maeda1，Shiro Baba2 1Department of Physics, Kitasato University School of Science 2Department of Urology, Kitasato University School of Medicine 3Department of Urology, The Jikei University

2P446 Proteomio analysis for urine samples ○Hiroshi Okusa1，Kazumasa Matsumoto1，Kazunari Yoshida1，Ryoujiro Kondo2，Kazuho Ashizawa2，Kazuteru Takafuji2，Oishi Masamichi2，Yoshio Kodera2，Tadakazu Maeda2，Shiro Baba1 1The Department of Urology, University of Kitazato 2The Department of Physics school of science,University of Kitazato

2P447 OdaibaDock: Protein-Protein docking system using a high-performance FFT algorithm on BlueGene/L. ○Tatsuya Yoshikawa1，Koki Tsukamoto1，Yuichiro Hourai1，Tadaaki Mashimo2，Yutaka Akiyama1 1Computational Biology Research Center, AIST 2Information and Mathematical Science Laboratory, Inc.

2P448 Improvement to a Protein-Protein Docking Algorithm by Introducing Layers in Molecular Expression Space ○Kazuya Sumikoshi1，Tohru Terada2，Shugo Nakamura1，Kentaro Shimizu1

1Dept. of Biotechnology, Grad. Sch. of Agricultural and Life Sciences, The Univ. of Tokyo 2Agricaltural Bioinformatics Research Unit, Grad. Sch. of Agricultural and Life Sciences, The Univ. of Tokyo

2P449 Improvement of the method for discriminating between biological and crystal-packing contacts in protein crystals. ○Yuko Tsuchiya1，Kengo Kinoshita1,2，Nobutoshi Ito3，Haruki Nakamura4 1HGC, IMS, Univ. of Tokyo 2SORST, JST 3Sch. Biomed Sci., Tokyo Med. & Dent. Univ. 4Inst. for Protein Res., Osaka Univ.

2P450 Correlation between sequence profiles and profiles derived from local structural classifications ○Kentaro Tomii1，Yoshito Sawada1，Shinya Honda1 1National Institute of Advanced Science and Technology (AIST)

2P451 Database analyses of protein structural changes induced by ligand binding ○Takayuki Amemiya1，Ryotaro Koike2，Yuki Imamura1，Sotaro Fuchigami1，Mitsunori Ikeguchi1，Akinori Kidera1 1Yokohama City Univ. 2Tokyo Inst. of Tech., BIRD JST

2P452 Membrane protein-ligand interactions: New insights from statistical analysis and molecular modeling studies. ○Xavier M. Suresh1，Michael M. Gromiha1，M. Suwa1 1Computational Biology Research Center (CBRC), AIST

2P453 Predictions of DNA-binding Proteins and Transcription Factor Targets in the Yeast Genome ○Antony Ngahu1，Takuya Ueno1，Shandar Ahmad2，Hidetoshi Kono3,4，Akinori Sarai1 1Dept. of Biosci. Bioinf., Kyushu Inst. of Tech. 2Dept. of Biosci., Jamia Millia Islamia Univ. 3Computational Biology, JAEA

4PRESTO, JST 2P454 Crystallization Experiment Support System in the Protein Research Group and RIKEN Structural Genomics/Proteomics Initiative (RSGI)

○Chie Takemoto1，Ryogo Akasaka1，Tomomi Uchikubo1，Aya Nishino1，Akiko Urushibata1，Machiko Yamaguchi1，T Terada1,2，F Konishi1，K Murayama1,4，Y Bessho1，M Shirouzu1，S Yokoyama1,2,3 1Genomic Sciences Center, RIKEN Yokohama Institute 2RIKEN Harima Institute at SPring8 3Graduate School of Science, University of Tokyo 4Tohoku University Biomedical Engineering Research Organization

2P455 Web service of the Protein Data Bank Japan (PDBj) ○Takashi Kosada1，Reiko Igarashi1,2，Yumiko Kengaku1,2，Yasuyo Morita1,2，Kanna Matsuura1,2，Masami Kusunoki1，Yukiko Shimizu1,2，Reiko Yamashita1,2，Haruki Nakamura1 1Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita-shi, Osaka 565-0871 2Japan Science and Technology Agency.

49. Ecology 2P456 The changing cell state of the transition from the predator-prey to symbiotic relationship between E.coli and D.discoideum

○Kumiko Kihara1，Shingo Suzuki2，Akiko Kashiwagi2，Itaru Urabe4，Tetsuya Yomo1,2,3 1Graduate School of Frontier Bioscience, Osaka University 2Graduate School of Information Science and Technology, Osaka University 3(Kaneko Complex Systems Biology Project,) ERATO, JST 4Graduate School of Engineering, Osaka University

50. Non-equilibrium and complex system 2P457 Hysteresis phenomena of discrete, reversible reaction networks

○Takeshi Sugawara1，Kunihiko Kaneko1 1The University of Tokyo

2P458 Multi-time-scale Dynamics of Networks of Threshold Elements ○Eiichiro Hoshino1，Kunihiko Kaneko1,2 1Dept. of Pure and Applied Sciences, Univ. of Tokyo 2ERATO Complex Systems Biology Project

2P459 Molecular Deficiency Induced Transitions in Catalytic Reaction Network ○Akinori Awazu1，Kunihiko Kaneko2 1Dept. of Physics, University of Tokyo 2Dept. of Pure and Applied Sciences, University of Tokyo

2P460 A new one-dimensional map which generates 1/f spectrum ○Kohei Nakajima1，Takashi Ikegami1 1Dept. of General Systems Sciences, The Graduate School of Arts and Sciences, Tokyo Univ.

2P461 Subjective time derived by dynamical interaction between A- and B-series ○Yukio-Pegio Gunji1，Taichi Haruna1，Daisuke Uragami1 1Dept. Earth & Planetary Scs. Kobe University

2P462 Robot control with amoeboid cells ○Soichiro Tsuda1，Yukio-Pegio Gunji1 1Kobe University

2P463 Lattice-Driven Cellular Automata: Computer Simulation Model of Local Semantics ○Daisuke Uragami1，Yukio-Pegio Gunji1 1Graduate School of Sience and Technology, Kobe University

2P464 The new route of 1/f spectrum in modified Bernoulli map ○Soya Shinkai1，Yoji Aizawa1 1Dept. of Appl. Phys., Waseda Univ.

2P465 State identification process and error propagation in a class of cellular automata ○Taichi Haruna1，Yukio-P. Gunji1 1Grad. School of Sci. and Tech., Kobe Univ.

2P466 A study of a reaction-diffusion-convection system by using cellular automaton model ○Mai Umeki1，Yasuhiro Suzuki1 1Graduate School of Information Science, Nagoya Univ.

2P467 Simulation of Morphogenesis using Pixel Cluster Automata ○Toshiya Nishi1，Tetsuya Matsuno1

1Dept. of electronics and information engineering, Ariake national college of technology 2P468 Phase-locked loop on circular distributed oscillators as a frequency decoder

○Nobuaki Nishiyama1,2 1Research Center for Higher Education, Kanazawa Univ. 2Graduate School of Natural Science and Technology, Kanazawa Univ.

2P469 Dependence of death mode condition on nonlinearity in coupled oscillator systems ○Hideaki Nemoto1，Yuki Kagawa1，Atsuko Takamatsu1 1Dept of Electrical Eng. and Bioscience, Waseda Univ.

2P470 Dynamical changing system parameter in one-dimensional map ○Q Zak1，Kohei Nakajima2，Soya Shinkai3，Kanata I-to4 1Quantum-Phase Electronics Center, School of Engineering, Tokyo Univ. 2Dept. of General Systems Sciences, Graduate School of Arts and Sciences, Tokyo Univ. 3Dept. of Applied Physics, Waseda Univ. 4JC Label

2P471 A liposome containing an autonomous DNA computing system inside ○Koh-ichiroh Shohda1，Daisuke Kiga1,3，Masahiro Takinoue1，Yoshiyuki Kageyama2，Tadashi Sugawara2，Akira Suyama1 1Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo 2Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo 3Department of computational intelligence and systems science, Tokyo Institute of Technology

2P472 Correlation, response, and violation of the fluctuation-dissipation relation in gene networks Yurie Okabe1，○Yu Yagi2，Masaki Sasai1 1Graduate School of Engineering, Nagoya University 2Graduate School of Information Science, Nagoya University

2P473 Phase dynamics of Brain Waves in Frequency Locking to Repetitive Flicker Stimuli ○Seiji Nishifuji1，Kentaro Fujisaki1，Shoto Tanaka1 1Yamaguchi University

2P474 Synthesis of a robust pace making network by oscillatory electrode assemblies ○Seiichiro Nakabayashi1，Nobuko Tanimura1，Toshikazu Yamashita2 1Department of Chemistry, Faculty of Science, Saitama University 2Department of Physiology, School of Medecine, Nihon University

2P475 Transitions between characteristic oscillation patterns in the plasmodium of Physarum polycephalum ○Takagi Seiji1，Ueda Tetsuo1 1Research Institute for Electronic Science, Hokkaido Univ.

2P476 Model of collective motion of motors in muscle oscillation ○Masako Ohtaki1，Shin'ichi Ishiwata1 1Department of Physics, School of Science and Engineering,

2P477 Coordination of cell growth and cell cycle progression in green algae ○Kazunori Matsumura1,2，Toshiki Yagi3，Kenji Yasuda2 1Dept. of Life Sci., Univ. Tokyo 2Dept. of Biomed. Info., Inst. Biomat. Bioeng., Tokyo Med. Dent. Univ. 3Dept. of Biol., Univ. Tokyo

2P478 Oscillatory feedback network constructed with an autonomous molecular computing system ○Masahiro Takinoue1,2，Daisuke Kiga3，Koh-ichiroh Shohda2，Akira Suyama2 1Dept. of Physics, Univ. of Tokyo 2Dept. of Life Sciences, Univ. of Tokyo 3Dept. of Computational Intelligence and System Science, Tokyo Institute of Technology

2P479 Heat-induced unidirectional movement of actin filament and its energy transformation ○Tomoaki Kawaguchi1，Hajime Honda1 1Dept. of BioEng., Nagaoka Univ. of Technol.

2P480 Relationship between Evolutionary Dynamics and Preservation of Molecular Structures in Metabolic Network ○Tomoharu Hogiri1，Naoaki Ono2，Chikara Furusawa1,2，Hiroshi Shimizu1 1Graduate School of Information Science and Technology, Osaka Univ. 2ERATO, JST

2P481 Characteristics of a Catalase-Hydrogen Peroxide Oscillation System and Its Application ○Satoshi Sasaki1 1School of Bionics, Tokyo Univ. Technol.

2P482 Self-organized mesoscopic-size patterns of microtubules driven by axonemal dynein c ○Kazuhiro Oiwa1，Dongyang Y. Li1,2，Rinna Nakamori1，Jia Lee1，Ferdinand Peper1，Hitoshi Sakakibara1 1Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology 2Dept. of Chemical and Materials Engineering, University of Alberta

2P483 The proposal of Locally Diagonal Mesh Transfer Effect in Vision ○Sohei Wakisaka1，Yukio P. Gunji1 1Grad School of science and Technology, Kobe Univ.

51. New methods and tools (II) 2P484 DNA analysis with TOF-SIMS

○Keigo Okada1，Junichi Kihara1，Satoka Aoyagi1 1Simane University

2P485 Development of an on-chip biomolecule parallel sorter using thermoreversible gelation polymer ○Hirokazu Sugino1，Yuki Nara2，Yoshitaka Shirasaki2，Takahiro Arakawa2，Syuichi Syoji2，Takashi Funatsu1 1Grad. Sch. of Pharmac. Sci., Univ. of Tokyo 2Grad. Sch. of Sci. and Eng., Waseda Univ.

2P486 Multiple cDNA quantification method based on DNA encoding technology ○Osamu Gotoh1，Kishoh Shiseki1，Atsushi Kameda1，Akira Suyama1 1Dept. of Life Sciences, Tokyo Univ.

2P487 Development of the SAAP simulation program for arbitrary amino-acid sequences ○Naoki Kimura1，Toshiya Minezaki2，Daisuke Yosida1，Michio Iwaoka1 1Department of Chemistry, School of Science, Tokai University 2School of Science, Tokai University

2P488 Evaluation of the SAAP simulation program by use of Met-enkephalin and C-peptide ○Ryuta Ooka1，Hironobu Hojo2，Yoshiaki Nakahara2，Naoki Kimura1，Norikazu Habiro1，Daisuke Yosida1，Michio Iwaoka1 1Department of Chemistry, School of Science, Tokai University 2Department of Applied Biochemistry, Tokai University

2P489 Sorting biomolecules in a 3D sheath flow microchannel using thermo reversible gelation polymer ○Yoshitaka Shirasaki2，Tokihiko Aoki2，Hirokazu Sugino1，Takahiro Arakawa2，Shuichi Shoji2，Takashi Funatsu1 1Grad. Sch. of pham. Sci., Tokyo Univ. 2Grad. Sch. of Sci. and Eng., Waseda Univ.

2P490 Photochemical DNA Manipulation via artifitial photoresponsive base ○Kenzo Fujimoto1，Yoshinaga Yoshimura1，Shinzi Ogasawara1，Masayuki Ogino1，Takehiro Ami1 1Japan Advanced Institute of Science and Technology

2P491 Development of a novel cell sorting system based on image recognition and microfabrication technology (I):System development.

○Akihiro Hattori1，Hideyuki Terazono1，Yoshie Kawase1,2，Kenji Yasuda1 1Institute of Biomaterials and Bioengineering Department of Biomedical Information, Tokyo Medical and Dental University, Tokyo 2ARIO TECHNO Co.Ltd., Amagasaki-city, Hyogo

2P492 Development of a novel cell sorting system based on image recognition and microfabrication technology (II): analysis based cell separation

○Yoshie Kawase1,2，Akihiro Hattori2，Hideyuki Terazono2，Kenji Yasuda2 1ARIO TECHNO Co.Ltd., Amagasaki-city, Hyogo 2Institute of Biomaterials and Bioengineering Department of Biomedical Information, Tokyo Medical and Dental University, Tokyo

2P493 Development of a novel cell sorting system based on image recognition and microfabrication technology (III). On-chip cell re-cultivation

○Hideyuki Terazono1，Akihiro Hattori1，Yoshie Kawase1,2，Kenji Yasuda1 1Institute of Biomaterials and Bioengineering Dept. of Biomedical Information, Tokyo Medical and Dental University, Tokyo 2ARIO TECHNO Co.Ltd., Amagasaki-city, Hyogo

2P494 Photosensitized thymine dimer splitting in DNA via carbazole nucleoside ○Yoshinaga Yoshimura1，Yoshiaki Kyoi1，Kenzo Fujimoto1 1Japan Advanced Institute of Science and Technology

2P495 Gel-phase NMR: a novel method for the protein NMR studies. ○Toshitatsu Kobayashi1，Masaki Mishima1，Toshiyuki Kohno2，Chojiro Kojima1 1Dept. of of Molecular Biology, Nara Institute of Science and Technology (NAIST) 2Mitsubishi Kagaku Institute of Life Sciences (MITILS)

2P496 Analysis of Surface Reaction of DNA Replication by SPR and its Application to Development of a High Speed DNA Sequencer

○Takahiro Kanemura1，Takeshi Iiduka1，Yuzuru Husimi1 1Dept of Functional Material Sci, Saitama Univ

2P497 Decision of SNP allele patterns by DNA computing technology ○Atsushi Kameda1，Yoko Sakai1，Kishoh Shiseki1，Takao Nakajima2，Akira Suyama1 1Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan 2NovusGene Inc., Olympus Corporation Utsugi Technical Research Institute, 2-3 Kuboyama-cho, Hachioji-shi, Tokyo 192-8512, Japan

2P498 Regulation of bioluminescence starting time by inoculated cell concentration ○Yuichi Sato1，Satoshi Sasaki2 1Grad, scool Bionics, Tokyo Univ of Technology 2Dept. of Bionics, Tokyo Univ of Technology

2P499 A novel approach for common line search based on sinogram-line features ○Ryoko Murayama1，Takuo Yasunaga1 1Depertment of Bioscience and Bioinformatics, Kyushu Institute of Technology

2P500 Acoustically induced electromagnetic radiation from biomedical tissues ○Kenji Ikushima1，Shunsuke Watanuki1，Susumu Komiyama1 1Dept. of Basic Science, Univ. of Tokyo

2P501 Molecular addressing enlarge the capacity of DNA molecular memory immobilized on a substrate ○Takahiko Endoh1，Masahiro Takinoue1,2，Akira Suyama1,2 1Dept. of Life Sci, Grad Sch of Arts & Sci, Univ. of Tokyo 2JST-CREST

2P502 Surface modification of diamond to prevent nonspecific protein adsorption toward the selective immobilization of target biomolecules

○Yosuke Amemiya1，Takatoshi Yamada2，Hiroshi Uetsuka2，Hideki Nakayama1，Shinichi Shikata2，Nobuo Shimamoto1 1Structural Biology Center, National Institute of Genetics 2Diamond Research Center, Advanced Industrial Science and Technology

2P503 Studies of dynamical transition of proteins with terahertz spectra ○Mariko Yamaguchi1，Kohji Yamamoto1，Masahiko Tani1，Masanori Hangyo1 1ILE, Osaka Univ.

2P504 The first observation of THz time-domain reflection spectra of lysozyme crystals ○Hideki Ichida1，Ryosuke Nakamura2，Yoko Sugawara3，Hisako Urabe4，Yasuo Kanematsu1,2 1Venture Business Laboratory, Center for Advanced Science and Innovation, Osaka University 2CREST, Japan Science and Technology Agency 3School of Science, Kitasato University, 4Tokyo Kasei Gakuin University

2P505 Protein tertiary structure prediction based on contact number prediction ○Takashi Ishida1，Shugo Nakamura2，Kentaro Shimizu2，Kenta Nakai1，Kengo Kinoshita1 1Human Genome Center, Univ. of Tokyo 2Dept. of Biotechnology, Univ. of Tokyo

2P506 Search for protein structures: Combined use of the Langevin dynamics and the class-sequence assessment ○HIKMET CETIN1，TAKESHI N. SASAKI2，MASAKI SASAI3 1CETIN HIKMET 2TAKESHI N. SASAI 3SASAI MASAKI

2P507 Experimental Validation of the Statistical Thermodynamic Model for Prediction of the Behavior of a Hairpin-forming DNA Machine

○Ken Komiya1，Satsuki Yaegashi3，Masami Hagiya2,3，John A. Rose4 1Interdis. Grad. Sch. of Sci. and Engi., TITech. 2Grad. Sch. of Info. Sci. and Tech., The Univ. of Tokyo 3JST-CREST 4Inst. of Info. Commu. Tech., Ritsumeikan APU

2P508 Development of electron microscopic image classification method by integration of principal component analysis with cluster analysis

○Hirosho Sumikura1，Takuo Yasunaga2 1Department of Bioscience and Bioinfomatics,Faculity of Computer Science and Systems Engineering,Kyusyu Institute of Technology 2Japan Science and Technology Agency

52. Bio-imaging 2P509 Muluti-color molecular imaging of transcription factors

○Kazumi Shinkura1，Kumiko Sakata-Sogawa2，Michio Hiroshima2，Makio Tokunaga1,2,3 1Natl. Inst. of Genetics 2RCAI, RIKEN. 3Grad. Univ. for Advanced Studies

2P510 Permeability change of the nuclear envelope ○Satoshi Shimozono1，Atsushi Miyawaki1 1Cell Function Dynamics, Brain Science Institute, RIKEN

2P511 Dissociation of pre-mRNA from nuclear speckle domain is ATP dependent process ○Yo Ishihama1，Hisashi Tadakuma1，Tokio Tani2，Takashi Funatsu3,4 1Graduate School of Science and Engineering, Waseda Univ 2Dept. of Biological Science, Kumamoto Univ 3Graduate Shool of Pharmaceutical Sciences, Tokyo Univ 4CREST, JST

2P512 A genetically-encoded indicator for RNA in living cells ○Kiwamu Takemoto1，Takeharu Nagai1 1Lab. Nanosystems Phisiology, Research Institute for Electronic Science, Hokkaido University

2P513 Real Time Imaging of Specific Messenger RNA in a Living Cell Using Artificial Nucleic Acids ○Kohki Okabe1，Yoshie Harada2，Takashi Funatsu1 1Grad. Sch. of Pharmac. Sci., Univ. of Tokyo 2The Tokyo Met. Inst. of Med. Sci.

2P514 Three-dimensional imaging of microtubule sliding generated by molecular motors ○Kana Mizutani1，Kazuhiro Oiwa2，Hitoshi Sakakibara2，Tomoko Masaike1，Takayuki Nishizaka1,3 1Gakushuin Univ., Fac. Sci., Dept. Phys. 2Kansai Adv. Res. Ctr. 3PRESTO, JST

2P515 Single molecule FRET imaging of enzymatic reactions at high concentrations of fluorescently labeled ligands ○Mitsuhiro Sugawa1，So Nishikawa2，Atsuko H. Iwane1，Takako Mimuro2，Toshio Yanagida1,2 1Graduate School of Frontier Biosciences, Osaka University, Osaka 2Japan Science and Technology Agency, Osaka

2P516 FRET sensor for phosphatidic acid to detect Phospholipase D activity ○Akira Nagasaki1，Kimiko Inotume1，Taro Q.P. Uyeda1 1Research Institute for Cell Engineering, AIST

2P517 Simultaneous dual-excitation ratiometry with light emitting diodes and high-speed liquid crystal shutters ○Takashi Fukano1，Satoshi Shimozono1，Atsushi Miyawaki1 1Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN

2P518 Phamret: An efficient highlighter protein based on photoconversion-mediated FRET for cell biological studies ○Tomoki Matsuda1，Atsushi Miyawaki2，Takeharu Nagai1 1Research Inst. for Electronic Science, Hokkaido Univ. 2Brain Science Institute, RIKEN

2P519 Ca2+ imaging of single living cells with bioluminescence resonance energy transfer (BRET) ○Kenta Saito1，Takeharu Nagai1 1Research Institute for Electronic Science, Hokkaido Univ.

2P520 Optical imaging of calcium transients in the muscle of ascidian larva ○Masashi Nakagawa1，Satoshi Shimozono2，Hideaki Mizuno2，Atsushi Miyawaki2 1Grad. School of Life Sci., Univ. of Hyogo, 2Lab for Cell Function Dynamics ,Brain Sci. Institute, RIKEN

2P521 Mitochondrial magnesium imaging ○Yutaka Shindo1，Takeshi Kubota1，Hirokazu Komatsu2，Kohji Hotta1，Hiroto Ogawa4，Koji Suzuki2,3，Kotaro Oka1 1Ctr. Biosci. Info., Fac. Sci. Tech., Keio Univ. 2Dept. Appl. Chem., Fac. Sci. Tech,, Keio Univ. 3JST-CREST 4Dept. Biol., Saitama Med. Univ.

2P522 Analysis of intracelullar binding process of glucocorticoid receptor using fluorescence correlation spectroscopy ○Shintaro Mikuni1，Mamoru Tamura1，Masataka Kinjo1 1Laboratory of Supramolecular Biophysics, RIES, Hokkaido University

2P523 Molecular dynamics of membrane-binding protein in living cells analyzed by total internal reflection fluorescence correlation spectroscopy

○Yu Ohsugi1，Mamoru Tamura1，Masataka Kinjo1 1Research Institute for Electronic Science, Hokkaido Univ.

2P524 Dual-color fluorescence cross-correlation spectroscopy using Keima and a fluorescence protein ○Takako Kogure1，Satoshi Karasawa1,2，Toshio Araki1,2，Kenta Saito3，Masataka Kinjo3，Atsushi Miyawaki1 1Cell Function Dynamics, BSI, RIKEN 2Amalgaam co. Ltd., 3Research Institute for Electronics Science, Hokkaido Univ.

2P525 Development of a microscopic platform for single-cell analyses using picoliter microwell array ○Yasuhiro Sasuga1,2，Tomoyuki Iwasawa1，Kayoko Terada1,2，Hiroyuki Sorimachi1，Osamu Ohara3,4，Yoshie Harada1,2 1The Tokyo Metropolitan Inst. of Med. Sci 2CREST, JST 3Dept. of Human Gene Res., Kazusa DNA 4RCAI, RIKEN Yokohama

2P526 Characterization of Quantum dot for immunofluorecence in living and fixed cells ○Songhua Li1,2，Tomonobu Watanabe2，Hideo Higuchi2，Noriaki Ohuchi1 1Dept. of Surgical Oncology, Graduate School of Medicine, Tohoku Univ. 2Biomedical Engineering Research Organization, Tohoku Univ.

2P527 Automatical detection and identification of various clinicopathological types of colorectal carcinoma tissues Serban Georgescu1,2，Yu Chen1，Masatoshi Makuuchi2，Munehiro Nakata3，○Wei Tang2 1Dept. Quantum Engineering and Systems Science, the Graduate School of Engineering, the University of Tokyo , Tokyo, Japan 2H-B-P Surg. Div., Dept. Surgery, the Graduate School of Medicine, the University of Tokyo, Tokyo, Japan 3Dept. Applied Biochemistry, Tokai University, Kanagawa, Japan

2P528 Uncompressed image storage system based on a personal computer and its application to microscopy of living cells ○Hisashi Fujiwara1，Takashi Ishiwata1，Etsuko Suzaki2 1Faculty of Information Sciences, Hiroshima City Univ. 2Graduate School of Biomedical Sciences, Hiroshima Univ.

2P529 Tensional Responses of Single Living Cells to External Stretch Visualized by SPM ○Kazushi Tamura1，Takeomi Mizutani1，Hisashi Haga1，Kazushige Kawabata1 1Division of Biological Sciences, Graduate School of Science, Hokkaido University

2P530 Measurement of mechanical properties of proteins from extreme thermophile and mesophile with AFM ○Jun'ichi Wakayama1，Satoshi Akanuma2，Toshio Ohtani1，Shigeru Sugiyama1 1Nano-Biotechnology Laboratory, National Food Research Institute 2Department of Molecular Biology, Tokyo University of Pharmacy and Life Science

2P531 Observation of synthesizing peptide of ribosome by high-speed AFM ○Akira Ando1，Toshihiko Matsuura2，Daisuke Yamamoto1,3，Toshio Ando1,3 1dept. of physics, Kanazawa Univ. 2Lab. of Biotechnology, Hokkaido Univ. of Education 3JST/CREST

2P532 High-resolution dynamic imaging of membrane proteins by high-speed AFM ○Hayato Yamashita1，Takayuki Uchihashi1,3，Kislon Voïtchovsky2，Sonia Antoranz Contera2，Daisuke Yamamoto1,3，John F. Ryan2，Toshio Ando1,3 1Dept. of Physics, Kanazawa Univ. 2Bionanotechnology IRC, Dept. of Physics, Oxford Univ. 3JST/CREST

2P533 Improvement of high-speed AFM scanner ○Ryo Nakakita1，Takayuki Uchihashi1,2，Toshio Ando1,2 1Dept. of Physics, Kanazawa Univ. 2CREST/JST

2P534 Direct driving of the high-speed AFM cantilever by photo-thermal expansion toward vide-Rate imaging of Biomolecules

○Takayuki Uchihashi1,2，Hayato Yamashita1，Noriyuki Kodera1，Ryo Nakakita1，Toshio Ando1,2 1Department of Physics, Kanazawa University 2CREST-JST

2P535 Structural analysis of biological specimen frozen in cryogenic solvent by atomic force microscopy ○Tatsuya Mori1，Natsumi Yamakawa1, Toshiaki Gotoh1, Tsutomu Kouyama1 1Department of Physics, Graduate School of Science,Nagoya University

2P536 Surface potential measurement of DNA, protein, lipid membrane at real space imaging ○Eriko Mikamo1,2，Fumihiko Yamada2，Takuya Matsumoto2，Tomoji Kawai2 1Discovery Res. Inst., RIKEN 2ISIR, Osaka Univ.

2P537 Mesoscopic model of the transient actin-sliding myosin crossbridges as captured by quick-freeze deep-etch replica electron microscopy

○Yoshitaka Kimori1，Yosuke Oguchi2，Norio Baba2，Eisaku Katayama1 1Inst. of Medical Science, The Univ. of Tokyo 2Dept. of Electric Engineering, Kogakuin Univ.

2P538 3D TEM of whole cell, Multi Section Electron Tomography ○Kazuhiro Aoyama1，Tomoko Takagi2，Issei Mabuchi2，Masako Osumi3 1Application Laboratory, FEI Company Japan Ltd. 2Dept. of Life Sciences, Graduate School of Arts and Sciences, Tokyo Univ. 3NPO: Integrated Imaging Research Support,

2P539 Development of GUI-based tools for three dimensional electron tomography ○Tatsunori Goto1，Rina Nagai2，Junichi Takagi3，Kenji Iwasaki3，Takuo Yasunaga1 1Department of Bioscience and Bioinformatics, Graduate School of Computer Science and System Engineering, Kyushu Institute of Technology 2Membrane Dynamics Project, RIKEN Harima Institute 3Institute for Protein Research, Osaka University

2P540 Single Particle Analysis of Image Data Acquired by Zernike Phase Contrast Transmission Electron Microscope ○Radostin Danev1，Kuniaki Nagayama1 1Okazak Institute for Integrative Bioscience, National Institutes of Natural Sciences (NINS)

2P541 Image reconstruction from electron diffraction patterns ○Kota Kawahara1，Takuro Sone1，Kazutoshi Gohara1 1Dept. of Applied Physics, Grad. Sch. of Engineering, Hokkaido Univ.

2P542 Bio technology application of SEM and STEM -WET STEM- ○Miki Nakamura1，Hiroshi Fujitani1 1FEI Company Japan, Ltd. Application Laboratory

2P543 Projection X-ray microscopy of Daphnia with eggs ○Susumu Tsuruta1，Tamami Kawasaki2，Masanobu Sakata3，Hideo Namiki3，Hideyuki Yoshimura1 1Department of Physics, Meiji Univ. 2Advanced Research Institute for Science and Engineering, Waseda Univ. 3Department of Integrative Bioscience and Biomedical Engineering, Waseda Univ.

2P544 Micro-CT of skin lesions infected with sarcoptic mites Kenichi Yamamoto1，Toshiaki Higashikawa2，Kunio Shinohara3，Susumu Tsuruta1，Hiromi Suzuki1，○Hideyuki Yoshimura1 1Dept. of Physics, Meiji Univ. 2Higashikawa's Dermatology Office 3Japan Synchrotron Radiation Research Institute

2P545 Imaging and direct structural analysis of biomolecules on the mammalian tissue surface using tandem mass spectrometry

○Shuichi Shimma1,2，Yuki Sugiura3，Mitsutoshi Setou2 1JSPS Research Fellow DC (Department of Physiological Sciences, the Graduate University for Advanced Studies)

2National institute for physiological sciences 3Department of Bioscience and Biotechnology, Tokyo Institute of Technology 2P546 Structural Analysis of Curly and Straight Human Hair Fibers by Scanning Microbeam SAXS

○Takashi Itou1，Yoshio Kajiura1，Yuya Shinohara2，Yoshiyuki Amemiya2

1Kao Corporation 2The University of Tokyo 2P547 Single cell based analysis on the polarity of Escherichia coli cells

○Satoru Ayano1，Ippei Inoue2，Daisuke Shiomi3，Ikuro Kawagishi3，Kenji Yasuda4 1Dept. of Life Science, Graduate School of Arts & Scinece, Tokyo Univ. 2Fermentation & Biotechnology Laboratories, Ajinomoto Co. 3Div. of Biological Science, Graduate School of Science, Nagoya Univ. 4Inst. of Biomaterials & Bioengineering, Tokyo Medical & Dental Univ.

2P548 Single-cell-based-observation of macrophage's phagocytic responses and the alteration of its morphology by activation

○Hirofumi Shindoh1，Kazunori Matsumura1，Hiroshi Ishimoto2，Katsunori Yanagihara2，Shigeru Kohno2，Kenji Yasuda1 1The Unibersity of Tokyo 2Nagasaki University

53. Bioengineering 2P549 Millennium Green of Japanese Jewel beetle: Unexpected Variability of Color Caused by Structural Change of Organic Multilayer

○Eiki Adachi1 1Nihon l'oreal K. K. R&D center

2P550 Controlling nano-porous structures of a diatom shell. ○Kazuo Umemura1，Xing Liao2，Shigeki Mayama3，Mohammed Gad4 1Tokyo Institute of Technology 2Tsinghua University 3Tokyo Gakugei University 4Ain Shams University

2P551 The effect of mechanical stimulation on osteogenic differentiation and calcification of cultured mesenchymal stem cells

○Daisuke Itoh1，Akimasa Fukui1，Naoki Sasaki1 1Dept. of Biological Science, Hokkaido Univ.

2P552 Reconstruction of Biomechanical Component using Insect Tissue for a Quasi-Living Actuator ○Yoshitake Akiyama1，Kikuo Iwabuchi2，Yuji Furukawa1，Keisuke Morishima1 1Dept. of Mechanical Systems Engineering, Tokyo Univ. of Agriculture and Technology 2Dept. of Applied Biological Science, Tokyo Univ. of Agriculture and Technology

2P553 Fabrication of One-dimensional Array of Cage-shaped Protein Yumiko Mishima1，○Hirotoshi Furusho2，Naohiro Kameta3，Mitsutoshi Masuda3，Midori Yamane1，Toshimi Shimizu3，Ichiro Yamashita1,2,4 1JST/CREST 2Graduate School of Materials Science, Nara Institute of Science and Technology 3AIST/NARC

4ATRL/Panasonic 2P554 Elucidation of the Mechanism of CdSe Nano Particles Synthesis in the Apoferritin Cavity

○Takuya Morioka1，Kenji Iwahori2，Ichiro Yamashita1,2,3 1NAIST 2CREST, Japan Science and Technology Agency 3ATRL, Matsushita Electric Industrial Co., Ltd.

2P555 Size controlled synthesis of fluorescent CdS nano-particles in the apoferritin ○Kenji Iwahori1，Ichiro Yamashita1,2,3 1CREST, Japan Science and Technology Agency 2Nara Institute Science and Technology 3ATRL, Matsushita Electric Industrial Co. Ltd.

2P556 Simulating Kinesin-Based Molecular Shuttle Movements under External Forces ○Takahiro Nitta1，Henry Hess2，Motohisa Hirano1 1Dept. of Math. and Design Eng., Gifu Univ. 2Dept. of Material Sci. and Eng., Univ. Florida

2P557 Artificial three strand β-helix protein as a nano tube ○Makoto Terauchi1，Shuji Kanamaru1，Fumio Arisaka1 1Grad.sch.of Biosci & Biotech, Tokyo Institute of Technology

2P558 Fabrication of gold nanoparticles using the cage protein ferritin ○Yasuyuki Otsuka1，Toru Konishi1，Hidetaka Aoki1，Akira Yamazaki1，Keiko Yoshizawa2，Ichiro Yamashita3，Hideyuki Yoshimura1 1Dept. of Phys., Meiji Univ. 2JST, CREST 3Material Sci., NAIST

2P559 In Vitro Synthesis of Calcium Nanoparticles Using the Protein Cage of Apoferritin ○Hiroko Fukano1，Hideyuki Yoshimura1，Mamoru Aizawa2 1Department of Physics, Meiji University 2Department of Applied Chemistry, Meiji University

2P560 Fabrication of molybdenum nanoparticles using the globular protein cavity ○Takayuki Kaneko1，Hiroko Fukano1，Yasuyuki Otsuka1，Toru Konishi1，Hideyuki Yoshimura1 1Dept. of Phys., Meiji Univ.

2P561 Selective and Autonomous Transport of Cargo Beads Using DNA Labeled Microtubules in Molecular Communication ○Satoshi Hiyama1，Takeshi Inoue2，Tomohiro Shima2，Yuki Moritani1，Tatsuya Suda1,3，Kazuo Sutoh2 1NTT DoCoMo, Inc. 2Dept. of Life Sciences, the Univ. of Tokyo 3Univ. of California, Irvine

2P562 Propagation of Tagged Liposome Containers in Molecular Communication ○Yoshihiro Sasaki1，Kohei Maruo1，Masashi Otsuki1，Mineo Hashizume1，Jun-ichi Kikuchi1，Yuki Moritani2，Satoshi Hiyama2，Tatsuya Suda2,3 1Nara Inst. Sci. and Technol. 2NTT DoCoMo, Inc. 3Univ. of California, Irvine

2P563 Detection of mechanical force to unbind complexes between cytoskeletal proteins and the antibodies immobilized on a nanoneedle

○Chikashi Nakamura1,2，Shingo Mieda2，Toshiya Sato2，Takanori Kihara1,3，Jun Miyake1,2,3 1Res. Inst. Cell Eng., AIST 2Dept. Biotechnol., Tokyo Univ. Agric. & Technol. 3CNBI, Univ. Tokyo

2P564 A low invasive and high efficient gene delivery method into a single cell using nanoneedle and AFM ○SungWoong Han1,2，Takanori Kihara1,2，Yosuke Imai3，Hideki Kamiishi3，Sayaka Kaneko3，Noriko Kotobuki1，Ohgushi Hajime1，Teruyuki Nagamune2，Chikashi Nakamura1,3，Jun Miyake1,2,3 1Res. Inst. Cell Eng., AIST 2CNBI, Univ. Tokyo 3Dept. Biotechnol., Tokyo Univ. Agric. & Technol

2P565 Biotin-Containing Phospholipid Vesicle Layer Adsorbed on a gold surface for Surface Plasmon Resonance Biosensing Yoshiko Ishizuka-Katsura1，Tadato Ban2，Ken-ichi Morigaki2，Shigeru Aoyama1,3,4，○Tetsuichi Wazawa1,4,5 1Grad. Sch. of Front. Biosci., Osaka Univ 2AIST 3OMRON 4CREST, JST 5Materials Process., Dept of Engin., Tohoku Univ

2P566 Simulation of Cargo Loss during Kinesin-Based Molecular Shuttle Transportation ○Akihito Tanahashi1，Takahiro Nitta1，Henry Hess2，Motohisa Hirano1 1Dept. of Math. and Design Eng., Gifu Univ. 2Dept. of Materials Sci. and Eng., Univ. Florida

2P567 Development of the addressing technique of DNA for microelectronic wiring ○Md. Masudur Rahman1，Hachiro Yasuda1，Michikiko Nakano1，Yukako Morii1，Shinji Katsura2，Akira Mizuno1 1Dept. Ecological Engineering, Toyohashi University of Technology 2Dept. of Biological and Chemical Engineering, Gunma University

2P568 The fabrication of wires with alternating material composition by utilizing the complementarity of DNA ○Shin-ichi Tanaka1，Wolfgang Fritzsche2，Yasushi Sako3，Toshio Yanagida1 1Graduate school of Frontier Biosciences, Osaka University 2 Institute for Physical High Technology 3Cellular Informatics Laboratory, RIKEN

2P569 Structural analysis of a DNA tetrahedron by electron cryomicroscopy ○Takayuki Kato1，Russell Goodman2，Richard Berry2，Andrew Turberfild2，Keiichi Namba1,3 1Grad. Sch. of Front. Biosci., Osaka univ. 2Dept. of Physics, Oxford univ. 3Dyn. Nano Pro., ICORP, JST

2P570 Microwave Assisted Rolling Circle Amplification Takeo Yoshimura1，Kunitada Nishida1，○Shokichi Ohuchi1 1Dept. of Biosci. and Bioinfor., Kyushu Inst. of Technol.

2P571 Kinetic Analysis of Regulatory Mechanism of Transcription-based DNA Memory Probe ○Miki Hirabayashi1，Hirotada Ohashi1，Tai Kubo2 1Dept. of Quantum Eng. and Systems Sci., Tokyo Univ. 2Neuroscience Res. Inst., AIST

2P572 Autonomous DNA computer for SNP analysis ○Daisuke Kiga1,2，Masahiro Takinoue1，Koh-ichiroh Shohda1，Akira Suyama1 1Dept of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo 2Dept of computational intelligence and systems science, Tokyo Institute of Technology

2P573 Emulsion based microreactor array in microchamber ○Hiroaki Kitagawa1，Wei Heong Tan1，Shoji Takeuchi1 1CIRMM, Institute of Industrial Science, The University of Tokyo

2P574 Bio-organic-inorganic ternary nanohybrids for DNA-barcode system ○Jae-Min OH1，Jung-Eun KIM1，Sang-Tae KIM1，Jin-Ho CHOY1 1Center for Intelligent Nano-Bio Materials(CINBM), Division of Nanoscience and Department of Chemistry, Ewha Womans University, Seoul 120-750, Korea

54. Biophysics and disease 2P575 Nonamer disruption simulation for model PrPSc using Go model of protein folding

○Hironori K. Nakamura1，Mitsunori Takano2，Kazuo Kuwata1 1CEID, Gifu Univ. 2Sch. of Sci. & Eng., Waseda Univ.

2P576 Metal ion binding to prion protein ○Yuji O. Kamatari1，Yoji Yoshimi2，Tomoharu Matsumoto1，Kota Kodama1，Kazuo Kuwata1 1Center for Emerging Infectious Diseases, Gifu Univ. 2Marubun Corporation

2P577 Quantitative analysis of protein mobility in diverse living cells based on multiple oligomeric GFP ○Changi Pack1，Masataka Pack1 1RIES, Hokkaido University

2P578 INFLUENZA VIRUS MOTION ON THE SUGAR-COATED SURFACE THAT MIMICS A CELL SURFACE ○Tatsuya Sakai1 1Dept. of Microbiol., Kawasaki Med. School

55. Drug design and delivery 2P579 Development of effective computer-aided drug design strategy by combining molecular docking and molecular dynamics simulation

○Noriaki Okimoto1，Atsushi Suenaga1，Noriyuki Futatsugi1，Ryoko Yanai1，Tetsu Narumi1，Yosuke Ohno1，Makoto Taiji1 1Genomic Sciences Center, RIKEN

2P580 Susceptibility of the hit ratio at in-silico drug screening to protein structures ○Katsumi Omagari1，Yoshifumi Fukunishi2，Satoru Kubota1，Haruki Nakamura2,3 1JBIRC, JBiC 2BIRC, AIST 3Inst. for Protein Res., Osaka Univ.

2P581 Fully Solvated Dynamical Docking Scheme for prediction of specific interactions of Protein, Ligand and Solvent Water Molecules

○Yohsuke Hagiwara1,2，Masaru Tateno1 1Center for Computational Sciences, University of Tsukuba 2Department of Bioscience and Biotechnology, Tokyo Institute of Technology

2P582 Calculation of binding free energy based on a variational principle for QSAR ○Yuichi Shine1，Takeshi Kikuchi1 1Dept. Biosci. and Bioinf., Col. Inf. Sci. and Eng., Ritsumeikan Univ.

2P583 On the Calculation Relative Binding Free Energy Between a Receptor and a Ligand based on a Free Energy Variational Principle

○Tatsunori Sakamoto1，Takeshi Kikuchi1

1Dept. Biosci. and Bioinf., Col. Inf. Sci. and Eng., Ritsumeikan Univ. 2P584 Bootstrap-based consensus scoring method for high-performance and robust drug screening by single scoring function

○Hiroaki Fukunishi1，Reiji Teramoto1，Jiro Shimada1 1Fundamental and Environmental Research Laboratories, NEC corporation

2P585 Toward Potent HDAC Inhibitor Design : Insights from Homology Modeling and Docking Simulation of HDAC and Its Inhibitors

○Yoshinori Hirashima1，Gururaj M. Shivashimpi1，Tamaki Kato1，Norikazu Nishino1，Satoko Maeda2，Tomonori G. Nishino2，Minoru Yoshida2 1Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology 2RIKEN

2P586 Development of HIV-1 RNaseH inhibitor by Computer-Assisted Drug Design ○Hideyoshi Fuji1，Junko Tatsumi1，Jun Komano2，Tyuji Hoshino1,3 1Grad. Sch. of Pharm. Chiba Univ. 2AIDS Res Ctr, NIID 3PRESTO, JST

2P587 Inhibitors and maturation mechanism of SARS main protease ○Chih-Jung Kuo1,2，Jim-Min Fang3，Chi-Huey Wong3，Andrew H.-J. Wang2，Po-Huang Liang2 1Taiwan International Graduate Program 2Institute of Biological Chemistry, Academia Sinica 3Genomics Research Center, Academia Sinica

2P588 Characterization of the complex for procaspase-3 with lactoferrin and caspase-3 with cathechin derivatives ○Hideaki Tsuge1，Eri Taniguchi1，Yasushi Sugimoto1，Nobuhiko Katunuma1 1Tokushima Bunri University

2P589 Structure and functional regulation of DNA complex formed with Cell-Signal responsive Gene Carrier ○Yuko T. Sato1,2，Kenji Kawamura1,2，Jun Oishi1,2，Takeshi Mori1,2,3,4，Takuro Niidome1,2,3,4，Yoshiki katayama1,2,3,4 1Department of Applied Chemistry, Faculty of Engineering, Kyushu University 2JST-CREST 3Center for Future Chemistry

421st COE 2P590 A novel mechanism for gene delivery to nucleus by non-viral vectors containing biosurfactant MEL-A

○Yoshikazu Inoh1，Yoshinobu Ueno2，Furuno Tadahide1，Hirashima Naohide2，Kitamoto Dai3，Nakanishi Mamoru1 1School of Pharmacy, Aichi Gakuin University 2Graduate School of Pharmaceutical Sciences, Nagoya City University

3National Institute of Advanced Industrial Science and Technology 2P591 Kinetics of membrane fusion between HVJ-envelope/DNA complexes and cells

○Nobuaki Yonekura1，Kana Yamamoto1，Takeshi Ohshio1，Masayuki Tadano1 1University of the Ryukyus

2P592 Maturation and Mechanism Based Inhibitor Design of SARS-CoV 3CL Protease: Implication in Antiviral 3C(L) Protease Drug Design

○Min-Feng Hsu1,2，Syaulan Yang3，Yao-Chen Teui1,4，Chih-Jung Kuo1，Kai-Ti Chang1,4，Shu-Jen Chen3，Hui-Lin Shr1,2，Tzu-Ping Ko1,2，Po-Huang Liang1,2,4，Ming-Chu Hsu3，Andrew H.-J. Wang1,2,4 1Institute of Biological Chemistry, Academia Sinica 2Core Facility for Protein X-ray Crystallography, Academia Sinica

3TaiGen Biotechnology 4Institute of Biochemical Sciences, National Taiwan University 2P593 Structures and mechanism of human glutaminyl cyclase, an enzyme possibly involved in Alzheimer's disease and osteoporosis

○Kai-Fa Huang1,2，Andrew H.-J. Wang1,2 1Inst. of Biol. Chem., Academia Sinica, Taipei 2Natl. Core Facility of High-Throughput Protein Crystallogr. Academia Sinica, Taipei

2P594 The antibiotic kasugamycin mimics mRNA nucleotides to destabilize tRNA binding and inhibit canonical translation initiation

○Tatsuya Kaminishi1，Frank Schluenzen2，Chie Takemoto1，Daniel N. Wilson2，Joerg M. Harms2，Kyoko Hanawa-Suetsugu1，Witold Szaflarski2，Masahito Kawazoe1，Knud H. Nierhaus2，Paola Fucini2，Shigeyuki Yokoyama1,3,4 1RIKEN Genomic Sciences Center 2Max Planck Institute for Molecular Genetics 3RIKEN Harima Institute at SPring-8 4Dept Biophys Biochem, Grad Sch Sci, Univ Tokyo