PowerPoint prepared by Mount Royal College Tissue: Part A … · 2019-11-04 · Skeletal Muscle: Attachments •Muscles attach: •Directly—epimysium of muscle is fused to the periosteum

PowerPoint® Lecture Slides prepared by Janice Meeking, Mount Royal College

C H A P T E R

Copyright © 2010 Pearson Education, Inc.

9 Muscles and Muscle Tissue: Part A


Warm Up 12/12/16

•Describe the major differences between cardiac, skeletal and smooth muscle in terms of appearance, control, function, and location!


Three Types of Muscle Tissue

1. Skeletal muscle tissue:• Attached to bones and skin

• Striated

• Voluntary (i.e., conscious control)

• Powerful

• Primary topic of this chapter



2. Cardiac muscle tissue:• Only in the heart

• Striated

• Involuntary

• More details in Chapter 18



3. Smooth muscle tissue:• In the walls of hollow organs, e.g., stomach,

urinary bladder, and airways

• Not striated

• Involuntary

• More details later in this chapter

Copyright © 2010 Pearson Education, Inc. Table 9.3


Special Characteristics of Muscle Tissue

•Excitability (responsiveness or irritability): ability to receive and respond to stimuli

•Contractility: ability to shorten when stimulated

•Extensibility: ability to be stretched

•Elasticity: ability to recoil to resting length


Muscle Functions

1. Movement of bones or fluids (e.g., blood)

2. Maintaining posture and body position

3. Stabilizing joints

4. Heat generation (especially skeletal muscle)


Skeletal Muscle

•Each muscle is served by one artery, one nerve, and one or more veins


Skeletal Muscle

•Connective tissue sheaths of skeletal muscle:• Epimysium: dense regular connective tissue

surrounding entire muscle • Perimysium: fibrous connective tissue surrounding

fascicles (groups of muscle fibers)• Endomysium: fine areolar connective tissue

surrounding each muscle fiber

Copyright © 2010 Pearson Education, Inc. Figure 9.1

Bone

Perimysium

Endomysium(between individualmuscle fibers)

Muscle fiber

Fascicle(wrapped by perimysium)

Epimysium

Tendon

Epimysium

Muscle fiberin middle ofa fascicleBlood

vessel

PerimysiumEndomysium

Fascicle(a

)

(b)


Skeletal Muscle: Attachments

•Muscles attach:• Directly—epimysium of muscle is fused to the

periosteum of bone or perichondrium of cartilage

• Indirectly—connective tissue wrappings extend beyond the muscle as a ropelike tendon or sheetlike aponeurosis

Copyright © 2010 Pearson Education, Inc. Table 9.1


Microscopic Anatomy of a Skeletal Muscle Fiber•Cylindrical cell 10 to 100 μm in diameter, up to 30 cm long

•Multiple peripheral nuclei

•Many mitochondria

•Glycosomes for glycogen storage, myoglobin for O2 storage

•Also contain myofibrils, sarcoplasmic reticulum, and T tubules


Myofibrils

•Densely packed, rodlike elements

• ~80% of cell volume

•Exhibit striations: perfectly aligned repeating series of dark A bands and light I bands


NucleusLight I band

Dark A band

Sarcolemma

Mitochondrion

(b) Diagram of part of a muscle fiber showing the myofibrils. Onemyofibril is extended afrom the cut end of the fiber.

Myofibril


Sarcomere

•Smallest contractile unit (functional unit) of a muscle fiber

•The region of a myofibril between two successive Z discs

•Composed of thick and thin myofilaments made of contractile proteins


Features of a Sarcomere

• Thick filaments: run the entire length of an A band• Thin filaments: run the length of the I band and partway into the A band• Z disc: coin-shaped sheet of proteins that anchors the thin filaments and connects myofibrils to one another•H zone: lighter midregion where filaments do not overlap •M line: line of protein myomesin that holds adjacent thick filaments together

Copyright © 2010 Pearson Education, Inc. Figure 9.2c, d

I band I bandA bandSarcomere

H zoneThin (actin)filament

Thick (myosin)filament

Z disc Z disc

M line

(c)

Small part of one myofibril enlarged to show the myofilamentsresponsible for the banding pattern. Each sarcomere extends fromone Z disc to the next.

Z disc Z discM lineSarcomere

Thin (actin)filament

Thick(myosin)filament

Elastic (titin)filaments

(d)

Enlargement of one sarcomere (sectioned lengthwise). Notice the myosin heads on the thick filaments.


Ultrastructure of Thick Filament

•Composed of the protein myosin• Myosin tails contain:

• 2 interwoven, heavy polypeptide chains

• Myosin heads contain:

• 2 smaller, light polypeptide chains that act as cross bridges during contraction

• Binding sites for actin of thin filaments

• Binding sites for ATP

• ATPase enzymes


Ultrastructure of Thin Filament

•Twisted double strand of fibrous protein F actin

•F actin consists of G (globular) actin subunits

•G actin bears active sites for myosin head attachment during contraction

•Tropomyosin and troponin: regulatory proteins bound to actin


Flexible hinge region

Tail

Tropomyosin

Troponin

Actin

Myosin head

ATP-bindingsite

Heads

Active sitesfor myosinattachment

Actinsubunits

Actin-binding sites

Thick filamentEach thick filament consists of manymyosin molecules whose heads protrude at opposite ends of the filament.

Thin filamentA thin filament consists of two strandsof actin subunits twisted into a helix plus two types of regulatory proteins(troponin and tropomyosin).

Thin filament

Thick filament

In the center of the sarcomere, the thickfilaments lack myosin heads. Myosin heads are present only in areas of myosin-actin overlap.

Longitudinal section of filamentswithin one sarcomere of a myofibril

Portion of a thick filament Portion of a thin

filament

Myosin molecule

Actin subunits


Sarcoplasmic Reticulum (SR)

•Network of smooth endoplasmic reticulum surrounding each myofibril

•Pairs of terminal cisternae form perpendicular cross channels

•Functions in the regulation of intracellular Ca2+ levels


T Tubules

•Continuous with the sarcolemma

•Penetrate the cell’s interior at each A band–I band junction

•Associate with the paired terminal cisternae to form triads that encircle each sarcomere


Myofibril

Myofibrils

Triad:

Tubules ofthe SR

Sarcolemma

Sarcolemma

Mitochondria

I band I bandA bandH zone

Z disc

Z disc

Part of a skeletalmuscle fiber (cell)

• T tubule• Terminalcisternaeof the SR (2)

M line


Triad Relationships

•T tubules conduct impulses deep into muscle fiber

• Integral proteins protrude into the intermembrane space from T tubule and SR cisternae membranes

•T tubule proteins: voltage sensors

•SR foot proteins: gated channels that regulate Ca2+ release from the SR cisternae



9A-2 Notes:


Warm Up! Explain the role and function of the T tubules and sarcoplasmic reticulum in relation to muscle contraction.


Contraction

•The generation of force

•Does not necessarily cause shortening of the fiber

•Shortening occurs when tension generated by cross bridges on the thin filaments exceeds forces opposing shortening


Sliding Filament Model of Contraction

• In the relaxed state, thin and thick filaments overlap only slightly

•During contraction, myosin heads bind to actin, detach, and bind again, to propel the thin filaments toward the M line

•As H zones shorten and disappear, sarcomeres shorten, muscle cells shorten, and the whole muscle shortens


I

Fully relaxed sarcomere of a muscle fiber

Fully contracted sarcomere of a muscle fiber

IAZ ZH

I IAZ Z

1

2


Requirements for Skeletal Muscle Contraction1. Activation: neural stimulation at a

neuromuscular junction

2. Excitation-contraction coupling: • Generation and propagation of an action

potential along the sarcolemma

• Final trigger: a brief rise in intracellular Ca2+ levels


Events at the Neuromuscular Junction

•Skeletal muscles are stimulated by somatic motor neurons •Axons of motor neurons travel from the central nervous system via nerves to skeletal muscles•Each axon forms several branches as it enters a muscle •Each axon ending forms a neuromuscular junction with a single muscle fiber


Figure 9.8

Nucleus

Actionpotential (AP)

Myelinated axonof motor neuron

Axon terminal ofneuromuscular junction

Sarcolemma ofthe muscle fiber

Ca2

+

Ca2+

Axon terminalof motor neuron

Synaptic vesicle containing AChMitochondrionSynapticcleft

Fusing synaptic vesicles

1 Action potential arrives ataxon terminal of motor neuron.

2 Voltage-gated Ca2+ channels open and Ca2+ enters the axon terminal.

Figure 9.8


Neuromuscular Junction

•Situated midway along the length of a muscle fiber

•Axon terminal and muscle fiber are separated by a gel-filled space called the synaptic cleft

•Synaptic vesicles of axon terminal contain the neurotransmitter acetylcholine (ACh)

•Junctional folds of the sarcolemma contain ACh receptors


Events at the Neuromuscular Junction

•Nerve impulse arrives at axon terminal

•ACh is released and binds with receptors on the sarcolemma

•Electrical events lead to the generation of an action potential

PLAY A&P Flix™: Events at the Neuromuscular Junction


Nucleus

Actionpotential (AP)

Myelinated axonof motor neuronAxon terminal of

neuromuscular junction

Sarcolemma ofthe muscle fiber

Ca2+ Ca2+


Synaptic vesiclecontaining AChMitochondrionSynapticcleft

Junctionalfolds ofsarcolemma

Fusing synaptic vesicles

ACh

Sarcoplasm ofmuscle fiber

Postsynaptic membraneion channel opens;ions pass.

Na+ K+

Ach–

Na+

K+

Degraded ACh

Acetyl-cholinesterase

Postsynaptic membraneion channel closed;ions cannot pass.

1 Action potential arrives ataxon terminal of motor neuron.

2 Voltage-gated Ca2+ channels open and Ca2+ enters the axon terminal.

3 Ca2+ entry causes some synaptic vesicles to release their contents (acetylcholine)by exocytosis.4 Acetylcholine, a

neurotransmitter, diffuses across the synaptic cleft and binds to receptors in the sarcolemma.

5 ACh binding opens ionchannels that allow simultaneous passage of Na+ into the musclefiber and K+ out of the muscle fiber.

6 ACh effects are terminated by its enzymatic breakdown in the synaptic cleft by acetylcholinesterase.


Destruction of Acetylcholine

•ACh effects are quickly terminated by the enzyme acetylcholinesterase

•Prevents continued muscle fiber contraction in the absence of additional stimulation


Events in Generation of an Action Potential

1. Local depolarization (end plate potential):• ACh binding opens chemically (ligand) gated

ion channels

• Simultaneous diffusion of Na+ (inward) and K+ (outward)

• More Na+ diffuses, so the interior of the sarcolemma becomes less negative

• Local depolarization – end plate potential



2. Generation and propagation of an action potential:• End plate potential spreads to adjacent

membrane areas• Voltage-gated Na+ channels open• Na+ influx decreases the membrane voltage

toward a critical threshold• If threshold is reached, an action potential is

generated



•Local depolarization wave continues to spread, changing the permeability of the sarcolemma

•Voltage-regulated Na+ channels open in the adjacent patch, causing it to depolarize to threshold



3. Repolarization:• Na+ channels close and voltage-gated K+

channels open• K+ efflux rapidly restores the resting polarity• Fiber cannot be stimulated and is in a

refractory period until repolarization is complete

• Ionic conditions of the resting state are restored by the Na+-K+ pump


Na+

Na+

Open Na+

Channel

Closed Na+

Channel

Closed K+

Channel

Open K+

Channel

Action potential++++++

++++++

Axon terminal

Synapticcleft

ACh

ACh

Sarcoplasm of muscle fiber

K+

2 Generation and propagation ofthe action potential (AP)

3 Repolarization

1 Local depolarization: generation of the end plate potential on the sarcolemma

K+

K+

Na+

K+

Na+

Wave of dep

olar

izat

io

n

Copyright © 2010 Pearson Education, Inc. Figure 9.9, step 1

Na+

Na+

Open Na+

Channel

Closed K+

Channel

K+

Na+

K+ Action potential

+++++++++++

+

Axon terminal

Synaptic

cleft

ACh

ACh


K+

1 Local depolarization: generation of the end plate potential on the sarcolemma1

Wave ofde

pola

rizat

ion


Na+

Na+

Open Na+

Channel

Closed K+

Channel

K+

Na+

K+ Action potential

+++++++++++

+

Axon terminal

Synaptic

cleft

ACh

ACh


K+

Generation and propagation of the action potential (AP)


2

1

Wave ofde

pola

rizat

ion


Na+

Closed Na+

Channel

Open K+

Channel

K+

Repolarization3


Na+

Na+

Open Na+

Channel

Closed K+

Channel

Action potential++++++

++++++

Axon terminal

Synaptic

cleft

ACh

ACh


K+

2 Generation and propagation ofthe action potential (AP)

3 Repolarization


K+

K+

Na+

K+

Na+

Wave of dep

olar

izat

io

n

Closed Na+

Channel

Open K+

Channel


Na+ channelsclose, K+ channelsopen

K+ channelsclose

Repolarizationdue to K+ exit

Threshold

Na+

channelsopen

Depolarizationdue to Na+ entry


Excitation-Contraction (E-C) Coupling

•Sequence of events by which transmission of an AP along the sarcolemma leads to sliding of the myofilaments

•Latent period:• Time when E-C coupling events occur

• Time between AP initiation and the beginning of contraction


Events of Excitation-Contraction (E-C) Coupling •AP is propagated along sarcolemma to T tubules

•Voltage-sensitive proteins stimulate Ca2+ release from SR • Ca2+ is necessary for contraction



Muscle fiberTriad

One sarcomere

Synaptic cleft

Setting the stage

Sarcolemma

Action potentialis generated

Terminal cisterna of SR ACh

Ca2+


Action potential is propagated alongthe sarcolemma and down the T tubules.

Steps in E-C Coupling:

Troponin

Tropomyosinblocking active sites

Myosin

Actin

Active sites exposed and ready for myosin binding

Ca2+

Terminal cisterna of SR

Voltage-sensitivetubule protein

T tubule

Ca2+

releasechannel

Myosincross bridge

Ca2+

Sarcolemma

Calcium ions are released.

Calcium binds to troponin andremoves the blocking action oftropomyosin.

Contraction begins

The aftermath

1

2

3

4


Steps inE-C Coupling:



T tubule

Ca2+

releasechannel

Ca2+

Sarcolemma

Action potential ispropagated along thesarcolemma and downthe T tubules.

1


Steps inE-C Coupling:



T tubule

Ca2+

releasechannel

Ca2+

Sarcolemma

Action potential ispropagated along thesarcolemma and downthe T tubules.

Calciumions arereleased.

1

2


Troponin

Tropomyosinblocking active sitesMyosin

Actin

Ca2+

The aftermath


Troponin


Actin


Ca2+

Calcium binds totroponin and removesthe blocking action of tropomyosin.

The aftermath

3


Troponin


Actin


Ca2+

Myosincross bridge

Calcium binds totroponin and removesthe blocking action oftropomyosin.

Contraction begins

The aftermath

3

4


Action potential is propagated alongthe sarcolemma and down the T tubules.

Steps in E-C Coupling:

Troponin

Tropomyosinblocking active sites

Myosin

Actin


Ca2+



T tubule

Ca2+

releasechannel

Myosincross bridge

Ca2+

Sarcolemma

Calcium ions are released.

Calcium binds to troponin andremoves the blocking action oftropomyosin.

Contraction begins

The aftermath

1

2

3

4


Role of Calcium (Ca2+) in Contraction

•At low intracellular Ca2+ concentration:• Tropomyosin blocks the active sites on actin

• Myosin heads cannot attach to actin

• Muscle fiber relaxes


Role of Calcium (Ca2+) in Contraction

•At higher intracellular Ca2+ concentrations:• Ca2+ binds to troponin

• Troponin changes shape and moves tropomyosin away from active sites

• Events of the cross bridge cycle occur

• When nervous stimulation ceases, Ca2+ is pumped back into the SR and contraction ends


Cross Bridge Cycle

•Continues as long as the Ca2+ signal and adequate ATP are present

•Cross bridge formation—high-energy myosin head attaches to thin filament

•Working (power) stroke—myosin head pivots and pulls thin filament toward M line


Cross Bridge Cycle

•Cross bridge detachment—ATP attaches to myosin head and the cross bridge detaches

• “Cocking” of the myosin head—energy from hydrolysis of ATP cocks the myosin head into the high-energy state


1

Actin

Cross bridge formation.

Cocking of myosin head.

The power (working) stroke.

Cross bridge detachment.

Ca2+

Myosincross bridge

Thick filament

Thin filament

ADP

Myosin

Pi

ATPhydrolysi

s

ATP

ATP

24

3

ADP

Pi

ADPPi


Actin


Ca2

+

Myosincross bridge Thick

filament

Thin filament

ADP

Myosin

Pi

1



ADPPi

2



ATP

3



ATPhydrolysi

s

ADPPi

4


1

Actin





Ca2+

Myosincross bridge

Thick filament

Thin filament

ADP

Myosin

Pi

ATPhydrolysi

s

ATP

ATP

24

3

ADP

Pi

ADPPi


Steps in E-C Coupling - Storytelling!

1. Fill in the blanks to the steps of the E-C “story” that I wrote out for you!

2. Define the terms in the word bank provided.

3. Retell the story by creating your own captions on the pictures provided. Be sure to label all * structures as well!


http://www.youtube.com/watch?v=hr1M4SaF1D4


To instigate the process… at the Neuromuscular Junction.

Neurotransmitters are released from

the axon terminal. As they diffuse across

the synaptic cleft, they attach to

Acetylcholine (ACh) receptors on the

sarcolemma.


Step 1

Net entry of Na+ initiates an Action

Potential which is propagated along the

sarcolemma and down the T Tubules.


Step 2

The action potential in the T tubule

activates voltage-sensitive receptors

which in turn trigger Ca2+ release from

the terminal cisternae of the

Sarcoplasmic Reticulum in the cytosol.


Step 3

Calcium ions bind to troponin; this

changes shape, removing the blocking

action of tropomyosin → therefore

leave the actin active sites exposed.


Step 4

Contraction! (The Cross Bridge

Cycle***) Myosin heads alternately

attach to actin and detach, pulling the

Actin filaments toward the center of the

sarcomere. Powered by ATP hydrolysis.


Step 4 broken down… 4.1 ***

Myosin head attached to the actin

myofilament, forming the cross bridge.


4.2*** Inorganic Phosphate (Pi) generated in the

previous contraction cycle is released,

initiating the power (working) stroke. The

myosin head pivots and bends as it pulls on

the actin filament, sliding it toward the

M-Line. Then ADP is released.


4.3***

A new ATP attached to the myosin

head, the link between myosin and actin

weakens, and the cross bridge

detaches.


4.4***

As ATP split into ADP and Pi, the

myosin head is energized (cocked into

the high-energy conformation) Ready to

start over!


Step 5

Removal of Ca2+ by active transport into

the sarcoplasmic reticulum (SR) after the

action potential ends.


Step 6

Tropomyosin blockage is restored,

blocking the myosin binding sites on

actin. Contraction ends and the muscle

fiber relaxes!!


Warm Up 1/9/17

→ Through what process is ATP made?!

• With oxygen =

• Without oxygen =

→ (Where does this process occur?)


What are the 6 Steps of EC Coupling?!

Remember that step 4 is our dance! (the cross bridge cycle!) - broken down into 4.1, 2, 3 and 4!


mitochondria

Synaptic vesicle

Acetylcholine (ACh)

Axon term

inal

Sodium rushes into the cell, sending an action potential down the length of the cell membrane (sarcolemma) and into the cell via T Tubules.

1

ACh Receptor

Sarcolemma


T Tubule

Sarcoplasmic Reticulum (SR) Voltage gated

receptor

Calcium 2 T tubules stimulate calcium release from the SR


3Calcium binds to troponin, unlocking the tropomyosin gate and exposing the myosin binding sites

Troponin

Tropomyosin

Myosin binding site

Actin

Myosin

Myosinhead


4Contraction begins! Actin is pulled towards the center of the sarcomere by myosin. (Also known as the Cross Bridge Cycle)

Let’s practice our dance!


Sarcoplasmic Reticulum (SR)

Calcium

5Calcium is removed from the cytosol and stored back in the Sarcoplasmic Reticulum for future use!


6Contraction ends! Tropomyosin gate and troponin lock are restored to their original positions, blocking the myosin sites on actin.

Documents

PowerPoint prepared by Mount Royal College Tissue: Part A … · 2019-11-04 · Skeletal Muscle: Attachments •Muscles attach: •Directly—epimysium of muscle is fused to the periosteum