STUDIES ON THE GROWTH OF FISH FUNGUS IN DIFFERENT CULTURE MEDIA
AND THEIR CHEMICAL CONTROLPRACHEE RAJPUT, REKHA CHAUHAN*Department
of Zoology & Applied AquacultureBarkatullah University, Bhopal
(462026)[email protected] INTRODUCTION Ornamental fishes suffer
from various diseases, most of them caused by different fungi which
grow on their bodies. Fungal infection in fish was first reported
during the mid 18th century (Arderon, 1748) and the credit of
chemical control of fish mycoses goes to Scale (1928) who
demonstrated the application of Murcurochrome in controlling fungal
infection in fishes. OBJECTIVE The research is done to measure the
comparative growths of fish fungi (causing infections in (Carrasius
auratus) in different culture media and their chemical control
using Formalin.MATERIALS AND METHODS A total number of 12 gold
fishes (Carrasius auratus) were collected from different pet shops
of Bhopal in the period of three months, September 2014 to November
2014. All the fishes were showed external symptoms of fungal growth
on body in form of cottony tufts and in some fishes parts of body
was found covered with lesions and inflammation caused due to
fungal attack. All the cultures were prepared on different agar
media and incubated in B.O.D incubator. Cultures were prepared on:-
S.P.S Agar Medium Potato Dextrose Agar (PDA) Corn Meal Agar (CMA)
Innoculation was done in Laminar flow in sterilized conditions.
Growth of colony was observed in 3-4 days. For full growth of
colony plates were kept for 8-10 days for incubation. These
petridishes were incubated at 28.0C to 30.0C temperature. Four
different concentrations of Formalin were used; these were 5mlL,
10mlL , 20mlL and 40mlL . Percentage inhibition = (T-C) x 100/T
Where, C = colony diameter (mm) of the control T = colony diameter
(mm) of the test plate.
CONCLUSIONColonial radial growth and sporulation were optimal on
S.P.S followed by CMA and PDA. Fusarium showed highest resistant
towards Formalin unlike other fungi. In the present investigation,
the following results were found out:Penicillium was accidentally
found which can only be grown on S.P.S medium; it didnt grow on any
other medium.In vitro studies of Formalin against the growth of
fungi showed that the used chemical can inhibit the growth of
fungal genera isolated but only in considerable high
concentrations.In conclusion, isolated fungi from diseased and
apparently healthy fish C. auratus, are considered normal mycoflora
but these are responsible to produce diseases under particular
conditions. Thus, these need more attention from the health point
of view of ornamental fish.
Graph: 1 showing percentage wise isolation of different fungus
from gold fishes (Carrassius auratus).
Fig: 2 [A, B, C and D] showing percent inhibition of growth of
Aspergillus fungus in 5mlL, 10ml/L , 20ml/L and 40ml/L
concentrations. [A]BCD
[A] [B]Fig: 3 (A and B) showing percent inhibition of growth of
Fusarium fungus in 5mlL and 10ml/Lrespectively. [C] [D]Fig: 3(Cand
D) ) showing percent inhibition of growth of Fusarium fungus in
20ml/L and 40ml/L respectively.
[A] [B]Fig: 4 (A and B) ) showing percent inhibition of growth
of Cladosporium fungus in 5mlL and 10ml/L respectively in CMA
medium.[C] [D]Fig: 4(C and D) ) showing percent inhibition of
growth of Cladosporium fungus in 20mlL and 40ml/L respectively in
CMA medium.
[A] [B]Fig: 5 (A and B) ) showing percent inhibition of growth
of Penicillium fungus in 5mlL and 10ml/L respectively in S.P.S
medium.[C] [D]Fig: 5 (A and B) showing percent inhibition of growth
of Penicillium fungus in 20mlL and 40ml/L respectively in S.P.S
medium.
PERCENT INHIBITIONPERCENT INHIBITIONMEAN DIAMETER OF
FUNGIConcentration of formalinMean diameter of fungal
growth0mlL950mm5mlL361mm10mlL228mm20mlL0mm40mlL0mmConcentration of
formalinMean diameter of fungal
growth0mlL950mm5mlL950mm10mlL950mm20mlL893mm40mlL807.5mmConcentration
of formalinMean diameter of fungal
growth0mlL950mm5mlL931mm10mlL760mm20mlL323mm40mlL0mmConcentration
of formalinMean diameter of fungal
growth0mlL20mm5mlL20mm10mlL12mm20mlL9mm40mlL5mmASPERGILLUS
FUSARIUMCLADOSPORIUM PENICILLIUM
Fig: 1 [A] Gold Fish (Carrassius auratus ) showing Aspergillus
infection.
Fig: 1 [B] Gold fish (Carrassius auratus ) was found infected
with Fusarium.
Fig: 1 [C] Cladosporium affecting the ventral surface of Gold
fish (Carrassius auratus).8
