FORENSIC MEDICINE AND MEDICOLEGAL PAPER

PRESIPITINE TEST

Clilical Internship Forensic Medicine and MedicolegalMedical Faculty Diponegoro University / RSUP Dr.

KARIADI SEMARANGNOVEMBER 7th- DECEMBER 3rd, 2011

CHAPTER ONE

In the most physical criminal case such as murder, persecution, rape, et cetera, may be found blood, semen,

saliva, urine, hair and other body tissues in the crime scene

In the most physical criminal case such as murder, persecution, rape, et cetera, may be found blood, semen,

saliva, urine, hair and other body tissues in the crime scene

can be very useful to reveal the crime scientifically

can be very useful to reveal the crime scientifically

Among the various body fluids, blood is the

most important because it is a biological fluid with

more specific potential characteristic for certain

human groups

Among the various body fluids, blood is the

most important because it is a biological fluid with

more specific potential characteristic for certain

human groups

The main purpose of forensic blood test is actually to help identify the owner of the blood, by comparing the blood spots found at the crime scene or clothes from the victim or

the crime suspect

The main purpose of forensic blood test is actually to help identify the owner of the blood, by comparing the blood spots found at the crime scene or clothes from the victim or

the crime suspect

In this case, the investigator must obtain clarity on the three main

things, namely:

Benzidine or luminol test Precipitine test Agglutination test

Based on the description above, the problem identification is …

Whether there is precipitate ring on human blood that reacted

with rabbit serum?

Research Purpose

Research Benefit

CHAPTER TWO

BLOOD

Blood is the fluid that found in all living things (except plants) that serves to send a high level of substances and oxygen to the body tissues, carrying chemical products of

metabolism, as well as the body's defense against viruses or bacteria.

BLOOD COMPOSITION

Antibodies

Antibodies or immunoglobulin (Ig) is a class of proteins

that formed plasma cells (B cell proliferation) due to contact with antigen.

Antibodies bind to specific antigens that create them.

Forensic Laboratory Examination

PREPARATION :Patches attached to

an object can be scraped off and

then soaked in physiological solution,or directly soaked with physiological sa

line solution when attach

ed to clothing

Presipitine test

CHAPTER THREE

Theoretical FrameworkAnimal(Rabbit)

Treated group(injected with human blood )

Control group

Presipitine Test

Hypothesis

There is precipitate ring on human blood that is given anti-human serum from

rabbit

CHAPTER FOUR

CHAPTER FIVE

Human Blood Identification

Materials :Human and animal (rabbit) blood 3cc and 1cc syringe, and 27 gauge needleAlcohol cotton 70%Test tubeCentrifugeNaCl

Method :Presipitin Test (specific test

to define to determine species)

Expected Result :Precipitate ring is formed

between anti-human serum and the extract, the ring is

muddy

Research Procedure

Forming anti-human serum

CHAPTER SIX

Serum

Kelinci

Hari ke-

1 3 5 7 9 11

1 - - - + + -

2 - - - + + -

3 - - - + + -

4 - - - + + -

5 - - - - - -

6 - - - - - -

CHAPTER SEVEN

SUMMARY

• Blood forensic laboratory examination can be a valuable information or certain

criminal case in the court• All blood sample in this research showas

that precipitate ring is formed in day 7

• Blood forensic laboratory examination can be a valuable information or certain

criminal case in the court• All blood sample in this research showas

that precipitate ring is formed in day 7

SUGGESTION

• Put more attention to confusing variable of the research in order to

get more accurate result • Put more detail literature about physiology condition of the rabbit

THANK YOU