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Your responsive CRO partner,
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MPI Research is the CRO that defines responsiveness, moving your drug development program
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GE HealthcareLife Sciences
For every success there’s a successor. It’s called
progress. So while we knew we had something
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substrate that operates with superior levels of
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its famous predecessor, making it an excellent
choice for CCD imagers.Welcome to ECL Prime.
Amersham and ECL are trademarks of GE Healthcare companies.
© 2010 General Electric Company – All rights reserved. First published November 2010.
GE Healthcare Bio-Sciences AB, Björkgatan 30, 751 84 Uppsala, Sweden.
Find out more about Amersham ECL Prime at
www.gelifesciences.com/eclprime
Comparison of Amersham ECL Prime and Amersham ECL Plus
detection of ß-catenin in NIH 3T3 whole cell lysates using different
dilutions of rabbit anti-ß-catenin and HRP-conjugated anti-rabbit IgG.
Thank you ECL™ Plus,you’ve been great.
Signal intensity sustained at higher antibody dilutions
Ab dilution Amersham ECL Prime Amersham ECL Plus
Primary Secondary 10 µg 156 ng 10 µg 156 ng
1:3000 1:300 000
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PathScan® Signaling Nodes
Multiplex IF Kitfrom Cell Signaling Technology
Immunofluorescent analysis of MCF7 (human breast adenocarcinoma) cells insulin-treated for 5 minutes, using PathScan® Signaling Nodes Multiplex IF Kit #8999.
PathScan® Signaling Nodes Multiplex IF Kit #8999 from
Cell Signaling Technology provides a novel multiplex assay to
simultaneously assess signaling through key pathway nodes
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#8999 Kit Targets Detection Dye Ex(max) (nm) Em(max) (nm)
Phospho-Akt (Ser473) Alexa Fluor® 555 555 565
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Phospho-S6 Ribosomal Protein (Ser235/236) Alexa Fluor® 647 650 665
TheA
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CLONING & MAPPINGDNA AMPLIFICATION
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Simplify DNA methylation analysis with MspJI
MspJI recognizes methylated and hydroxymethylated DNA and cleaves
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Sigma and Sigma-Aldrich are registered trademarks belonging to Sigma-Aldrich Co. and Sigma-Aldrich Biotechnology, LP. MISSION is a registered trademark of Sigma-AldrichBiotechnology LP and Sigma-Aldrich Co. SwitchGear Genomics is a trademark of SwitchGear Genomics. GoClone is a trademark of SwitchGear Genomics
Bioidentify.Introducing the MISSION®
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A $22.50 valu�—you�s free wh�n you
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Some of the Many Reasons to Attend SBS 2011:
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Analytical & QuantitativeLight MicroscopyMay 4 - May 13
Biology of the Inner EarAugust 7 - August 27
Biology of Parasitism:Modern ApproachesJune 17 - August 6
BioMedical Informatics1st Session: May 29 - June 42nd Session: September 18 -September 24
Embryology: Concepts& Techniques in ModernDevelopmental BiologyJune 4 - July 17
Frontiers in Reproduction:Molecular & Cellular Concepts& ApplicationsApril 30 - June 12
Frontiers in Stem Cells andRegenerationOctober 2 - October 8
Gene Regulatory Networksfor DevelopmentOctober 11 - October 22
Methods in ComputationalNeuroscienceJuly 31 - August 28
Microbial DiversityJune 11 - July 28
Molecular Biology of AgingJuly 24 - August 13
Molecular Mycology:Current Approaches to FungalPathogenesisAugust 3 - August 19
Neural Systems & BehaviorJune 4 - July 31
NeurobiologyJune 4 - July 31
NeuroinformaticsAugust 13 - August 28
Optical Microscopy & Imagingin the Biomedical SciencesOctober 11 - October 21
Physiology: Cell &Computational BiologyJune 11 - July 31
FOR MORE INFORMATION CONTACT:
Admissions Coordinator; [email protected];(508) 289-7401, MBL, 7 MBL Street,Woods Hole, MA 02543
www.mbl.edu/education
2011 Courses
The MBL is an Equal Opportunity/Affirmative Action Institution.
Substantial financialassistance is availablefor many of our courses!
Strategies and Techniques forAnalyzing Microbial PopulationStructuresAugust 3 - August 13
Summer Program inNeuroscience, Ethics, & Survival(SPINES)June 18 - July 16
Workshop on MolecularEvolutionJuly 24 - August 3
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1700 17 DECEMBER 2010 VOL 330 SCIENCE www.sciencemag.org/products
NEW PRODUCTS FOCUS: RNAi
LIFE SCIENCE TECHNOLOGIES AAAS/Science Business Office
Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations
are featured in this space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any
products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.
Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information.
TRANSFECTION REAGENTS Effective and nontoxic DNA and small interfering RNA (siRNA) de-
livery is essential for reliable scientifi c results. jetPRIME is a new
versatile and powerful DNA and siRNA transfection reagent for day-
to-day experiments. jetPRIME ensures high DNA transfection effi -
ciency and excellent gene silencing in a variety of adherent cells.
jetPRIME is ideal for DNA/siRNA cotransfection and is very gentle to
cells since it requires low amounts of nucleic acid and reagent during
transfection. The jetPRIME protocol is easy-to-follow and compatible
with the use of serum and antibiotics during transfection.
Polyplus Transfection
For info: 508-315-9629 www.polyplus-transfection.com
siRNA SILENCING KITEnabling researchers to perform effi cient small interfering RNA (siR-
NA) silencing (gene knockdown) experiments in small animal mod-
els, Invivofectamine 2.0 Reagent and Ambion In Vivo siRNA provide
strong and sustained knockdown of in vivo protein expression in
an easy-to-use kit. These tools accelerate the use of RNA interfer-
ence to effi ciently study gene function in animal models. Targeted
gene silencing has been demonstrated to last for more than three
weeks with a single application of Invivofectamine 2.0 Reagent
and the positive control Ambion In Vivo siRNA. The new reagents
provide a powerful alternative to the conventional use of knockout
mouse models, which can take nearly 10 months to develop. Sold
as part of a 1 ml starter kit or a 5 ml kit, Invivofectamine 2.0 Reagent
and Ambion In Vivo siRNA are synthetic, contain no components
of viral origin, and show minimal to no toxicity after extensive
initial studies.
Life Technologies (Invitrogen)
For info: 800-955-6288 www.invitrogen.com
IN VIVO TRANSFECTION KITThe PEG-Liposome siRNA In Vivo Transfection Kit is designed for
in vivo transfection of negatively charged molecules—RNA, DNA,
and small proteins. Transfection is one of the major laboratory
methods used to introduce DNA and RNA molecules into cells and
tissues. This technique makes it possible to cross cellular barriers
and deliver a gene or a small interfering RNA (siRNA) into cells for
research or therapeutic purposes. The novel PEGylated liposome-
based siRNA delivery kit is optimized for directed RNA interference
induction by effi cient delivery of functional small RNA molecules
(siRNA, shRNA, miRNA) into tissues. The liposome component
provides in vivo siRNA protection due to effi cient siRNA-liposome
encapsulation. A remarkable feature of this reagent is reduced
innate immune response and low cytotoxicity due to biodegradable
PEG modifi cation.
Altogen Biosystems
For info: 800-658-7009 www.altogen.com/mirna.php
RNA ISOLATION When working with stored materials, researchers often need to
isolate RNA from samples that have been stored as formalin-fi xed
paraffi n-embedded (FFPE) tissue blocks. Although the tissue struc-
ture of FFPE samples will have been maintained for histological anal-
ysis, damage to the nucleic acid may have occurred through the fi xa-
tion, embedding, and storage processes impeding measurements
of gene expression levels. ExpressArt FFPE RNAready overcomes
the limitations associated with RNA degradation and interference
from RNA cross-linking in FFPE tissues. The FFPE RNAready kit ef-
fi ciently isolates and preserves mRNA using a specially developed
lysis solution and an innovative universal inhibitor of RNases that dis-
places tightly bound RNA from high-molecular-weight aggregates.
Subsequent treatment with a unique de-modifi cation reagent re-
verses formalin induced cross-links, resulting in total RNA optimized
for reverse transcription reactions and subsequent downstream ap-
plications. FFPE RNAready together with ExpressArt RNA amplifi -
cation TRinucleotide primer technology is now available to amplify
microgram quantities of high quality RNA from very small amounts
of degraded total RNA, with minimal loss of sequence.
AMSBIO
For info: +44-0-1235-828200 www.amsbio.com
siRNA PREMIXES
With FlexiTube siRNA Premix, small interfering RNA (siRNA) for your gene of interest and
highly effi cient transfection reagent are already premixed and provided in a single tube. Flexi-
Tube siRNA Premix includes a specialized buffer that increases the stability of siRNA-reagent
complexes. The ratio of reagent to siRNA in FlexiTube siRNA Premix is preoptimized to provide
the highest transfection effi ciency and gene knockdown. FlexiTube siRNA Premix is ready-to-
use for cell transfection. Simply add to cells and incubate. With FlexiTube siRNA Premix, RNA
interference experiments get off to a faster start, as there is no need to optimize siRNA-to-
reagent ratio. Tedious optimization experiments involving multiple transfections are minimized
or eliminated. Multiple transfections can be performed from a single FlexiTube siRNA Premix,
reducing variability, and enabling consistency across experiments and more reliable results.
Qiagen
For info: 800-426-8157 www.qiagen.com
MemberCentral.aaas.org
AAASMemberCentralINTRODUCING
AAASMemberCentral is a newwebsite focused on helping you— the scientists, engineers,
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On MemberCentral you can contribute to discussion groups or blogs, participate in a
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The exclusive new website for the AAAS member community.
Every lab needs one.
Are you that type? Always looking for ways to simplify and streamline lab
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FOR RESEARCH USE ONLY. Not intended for any animal or human therapeutic or diagnostic use.
©2010 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.
For those who require IVD-marked devices, the 3500 Dx and the 3500xL Dx Genetic Analyzers and system accessories meet the requirements of the In Vitro Diagnostics Medical Devices
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