Complex, multi-step methods of protein sample preparation have inherent challenges of time, contamination and sample loss.

Preparing labelled samples over extended time periods with a complex method leads to variation in results.

This challenge is exacerbated with large numbers of samples and small starting amounts of input material.

PreOmics improved sample technology (iST) enables robust and reproducible sample preparation.

The iST method provides significant time savings as complex workflows previously requiring many different reagents taking up to 48 hours are replaced by a single kit containing all standardised reagents.

The iST kit allows protein sample preparation for mass spectrometry analysis, whether manually, semi or fully automated, in a 2-hour time window.

THE CHALLENGE

PREOMICS VS COMMON PROTOCOL

THE SOLUTION

SAMPLE PREPARATIONSIMPLIFIED

iST TECHNOLOGY

Welcome to the range of PreOmics sensitive, fast and easy to use sample preparation kits.

10 min at 95ºC

10 min at 95ºC

LysisDenaturation

Common protocol:

PreOmics protocol:

LysisDenaturation,

Reduction,Alkylation

Reduction Alkylation Precipitation Lys-Cdigestion

Lys-C &Trypsin

digestion

Trypsindigestion

Peptidepurification

Peptidepurification

MassSpectrometer

MassSpectrometer

Resultanalysis

Resultanalysis

45 min at 50ºC 20 min at RT 12h at -20ºC 4h at 37ºC 18h at 37ºC 2h at RT

1h at 37ºC 1h at RT

Fast, reliable, reproducible peptide clean–up for MS based proteomics

iST* = Fast, reliable, reproducible protein sample preparationiST-NHS** = Chemical labelling (iTRAQ and TMT*) and protein sample preparation

combined The PreON platform was built to address the demands of reproducible, robust and sensitive sample preparation. Free up your valuable time, standardize your results and increase your productivity.

Cell lines Fresh & FFPE tissues Body fluids (serum, plasma, CSF, urine) Bacteria, yeast & plant tissues

iST & iST-NHS KITS PHOENIX KIT

SAMPLE TYPESiST IMPROVED WORKFLOWS

AUTOMATION

excellent sensitivity for 1-100 µgreproducible, reliable & robust

proprietary peptide cleanup avoids MS downtimefast & versatile with various sample typesiS

T Ki

t

convenient labeling within the iST workflow

& labelled peptides in less than 4 hoursfrom starting material to clean

iST-

NH

S Ki

t

Sam

ple

Prep

arati

on

saves 50% labeling reagent

Lyse Digest PurifyReduce &Alkylate

Wash &Elute

LysC &Trypsin

1 32

10 min 60 min 60 min

95 °C 37 °C RT

Lyse Digest LabelReduce &Alkylate

Label &Quench

LysC &Trypsin

1 32

PurifyWash &

Elute

4

RT

60 min10 min 60 min 60 min

95°C 37°C RT

all advantages of iST Kit

highly efficient cleanup of peptides

exceptional peptide recoveryeasy-to-use, sensitive and fastPh

oeni

x Ki

t

Pepti

de

Clea

nup

removes salts, sugars, polymers, detergents, lipids, etc. Load Purify Elute

control pH & load EluteWash

1 32

5 min 30 min 10 min

RT RT RT

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Select a protocol or scan the kit

Automates your label-free and chemical labelling workflows

Eliminates manual processing steps (only 5 min hands-on time)

Generates standardized and highly reproducible results

Works seamlessly with ready-to-go PreOmics iST and iST-NHS kits

RELAX

REALIZE

Highly efficient workflow

Cleanest peptidesready to go!

Results in ready to use clean peptides45-minute processing timeSpin column format compatible with standard lab equipmentFrom 1-96 samples at a time; manual to fully automated options

Dual clean up step removes hydrophilic & hydrophobic contaminantsRemoval of >99.5% standard detergents, >85% Tween 20 and up to 90% peptide recoveryNo generation of unwanted TMT side reaction productsResults in ready to measure peptides & less Mass-Spectrom-etry instrument downtime

www.preomics.com | info@preomics.com

REALIZECleanest peptides ready to go!

No generation of unwanted TMT side reaction products**Compatible with standard lab equipment

Results in ready to measure peptides & less mass spectrometry equipment downtime

SMILE

RELAX

Proteomics quality results each and every time

Highly efficient workflow

High reproducibility: R2=0.9Working range: 1-100μgIncreased number of peptides and protein identifications

Streamlined method for isobaric labelling**

Manual to fully automated options

No need for peptide clean up prior to labelling**

1h* hands-on time (1,5h**) Single step incorporating lysis, reduction & alkylationAll-in-one solution, all reagents provided*

Dual clean up step removes hydrophilic & hydrophobic contaminants

* iTRAQ and TMT Labels not supplied