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Presentation of Expression of Certain E nzymes in a Panel of Human Cancer C ell Lines. By Sara Soth. Background Information. Adenocarcinoma Cancer Cell Line. P450 Enzymes Antioxidative Enzymes Drug Metabolism (Enzymes) CYP1A1 and CYP1B1 MnSOD Cancer Cell Lines. - PowerPoint PPT Presentation
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Presentation of Expression of Certain Enzymes in a Panel of Human Cancer
Cell Lines
By Sara Soth
Background Information
P450 Enzymes Antioxidative Enzymes Drug Metabolism (Enzymes) CYP1A1 and CYP1B1 MnSOD Cancer Cell Lines
Adenocarcinoma Cancer Cell Line
Background Information Continued
P450 Enzymes Includes CYP1A1 and CYP1B1 Catalyze the oxidation of organic
substances.
Antioxidative Enzymes Lack of enzyme causes oxidative
stress, stress will then cause damage or kill cell.
Balance needed.
Background Information Continued
Drug Metabolism (Enzymes)
Equivalent of xenobiotic metabolism. Works with P450 enzymes. Affect on chemotherapy treatment
Background Information Continued
MnSOD Mitochondrial Superoxide Dismutase mRNA Protein
Background Information Continued
Cancer Cell Lines A2780 – Ovarian Cancer
• Carcinoma SKOV-3 – Ovarian Cancer
• Caucasian Adenocarcinoma LOVO and DLD-1– Colorectal Cancer
• Adenocarcinoma MCF-7 and MDA-MB-231– Breast Cancer
• Caucasian Adenocarcinoma HeLa – Cervical Cancer
• Epitheloid Carcinoma C33A – Cervical Cancer
• Carcinoma
Background Information Continued
Methodology RNA isolated by Chomczynski and Sacci
methods. Grown on cell plates Collected at 60% confluent Put in Tris-glycine gel with SDS-page. Transfer to Nitrocellulose Perform immunodetection with Rabbit
polyclonal anti-CYP1B1Ab and MnSOD Ab Densitometric quantification of band
amplification by ImageJ 1.46 software for imaging.
Resources http://www.ncbi.nlm.nih.gov/gene/1543 http://www.ncbi.nlm.nih.gov/gene/1545 http://ghr.nlm.nih.gov/gene/CYP1B1 http://
medical-dictionary.thefreedictionary.com/procarcinogen http://www.aafp.org/afp/2007/0801/p391.pdf http://www.merriam-webster.com/medical/immunostaining http://
cancerres.aacrjournals.org/content/57/14/3026.full.pdf http://
www.ncbi.nlm.nih.gov/pmc/articles/PMC2990475/pdf/nihms236168.pdf
http://www.ncbi.nlm.nih.gov/pubmed/20840865 http://www.oxidativestressresource.org/ http://www.ncbi.nlm.nih.gov/pubmed/2440339