Presin Termodinmica

Nada en Biologa tiene sentido a menos que se entienda en trminos de Evolucin

T. Dobzhansky

Teoras actuales de la Evolucin MolecularTeora Neutral (Kimura) La gran mayora de cambios evolutivos a nivel molecular son originados neutralmente al asar y fijados por deriva gentica sin someterse a una seleccin natural.

Teora Seleccionista Los cambios evolutivos a nivel molecular son seleccionados para mantener o mejorar el grado de adaptacin.

Teora Mutacionalista Los cambios evolutivos a nivel molecular obedecen a presiones mutacionales las cuales pueden variar entre y dentro de especies.

Una manera simple de entender la evolucin

DATOS: Alineamiento de secuencias de genesCmo podemos transformar esta informacin a un contexto histrico?

Los objetos vivientes estn compuestos por molculas inertes

Albert Lehninger

El problema es:

Cmo estas molculas confieren la admirable combinacin de caractersticas que denominamos vida???

Cmo es que un organismo vivo aparece ser ms que la suma de sus partes inanimadas???

La Fsica procura entender y reducir la Biologa en leyes fundamentales

Pero este es un problema muy complicado !

Las leyes Fsicas podrn afectar la dinmica del proceso de replicacin del ADN?

Hiptesis de la Presin TermodinmicaADN+ADN polimerasa tratados como un sistema macrocannico Uso de la distribucin de Boltzmann para calcular la probabilidad de incorporacin de un nucletido particular durante la replicacin del ADN

Durante el proceso de reparacin del ADN tambien estara actuando la presin termodinmica

Energa Electrosttica de una vecindad de 5 pb 1 2 3 4 5 ......... 1st strand position 5 A - G - T - C - A 3 3 T - C - X - G - T 5 10 9 8 7 6 ......... 2nd strand position X : any of the 4 nucleotides

E 12 = 1 [ p1 . p2 -- 3 ( r.p1 )( r.p2 ) ] 4pKeo r 3 r 5

_919146432.unknown

MA = -134.1 x 10-24 C m2 /s EAA = -0,56642 x 10-4 eV EAA= -0,481 x 10-3 eV

Energa Magnetosttica entre nucletidos

A T -0.34 eVG C -0.43 eVEnerga de Puente de Hidrgeno entre Pares de Watson-Crick

Interacciones tipo Puente de Hidrgeno en missmatches

(eV) (Kcal/mol) A-T = -0.34 -7.84 G-C = -0.44 -10.14 C-A = 0.21 4.84 T-G = 0.13(I) -0.40(II) 2.99(I) -9.22(II) ** T-C = -0.14 -3.22 A-G = -0.27 -6.22 * T-T = 0.02(I) -0.16(II) 0.46(I) -3.68(II) ** C-C = -0.01(I) -0.14(II) -0.23(I) -3.22(II) ** A-A = 0.23 5.30 * G-G = 0.54 12.45 *

(*) These energies correspond to free purine-purine pairs, however when they are embedded in a double helix, these base pairs strongly disturb the double helix configuration and become energetically forbidden(**) Cases (I) y (II) correspond to the two configurations that can adopt these bases. The cases (II) are forbidden in a double helix configuration, whereas cases (I) are not.

Energa tipo Puente de Hidrgeno entre pares de bases cannicas y no-cannicas en vaco (k=1)

Energas que afectan la estabilidad de la vecindad de nucletidosEnergas de enlaceEnlace covalenteEnerga angular de enlaceEnerga de ngulo dihedro

Energas de no-enlaceElectrostticaVan der WaalsPuentes de Hidrgeno Efecto hidrofbicoMagnetostticas

Simular un proceso de replicaciones consecutivas por muchas generaciones en donde se modelan mutaciones puntuales

Clases de ADNADN funcional codanteADN funcional no-codante (promotores, enhancers, intrones, genes ribosomales, etc.)ADN no-funcional (basura)

Criterios de viabilidad para las mutaciones puntuales

(Presin de seleccin nula)Cladognesis (especiacin)ADN funcional codante: conserv. Fam. Aminoac.ADN funcional no-codante: ???ADN no-funcional: Ninguna restriccinAnagnesis (envejecimiento)ADN funcional codante: conserv. Aminoac.ADN funcional no-codante: ???ADN no-funcional: Ninguna restriccin

Variacian del %GC en el tiempoRestriccin: Conservacin del AA y de la familia de AA

Cladognesis a partir de anagnesis

Efecto de la Temperatura sobre la variacin del contenido de GC P(X = M ) -----------> 1 , when T increases. P(X = N )

Variacin del %GC en cada posicin de codnRestriccin: Conservacin de familia de AA

Energa de los tripletes de AND en funcin de la vecindad

Sheet1

XC T GY

( eV )

C-8.47E-03C

C-5.90E-03T

C-8.43E-03G

C-7.11E-03A

T-6.46E-03C

T-3.89E-03T

T-6.42E-03G

T-5.11E-03A

G-7.24E-03C

G-4.67E-03T

G-7.20E-03G

G-5.88E-03A

A-5.42E-03C

A-2.85E-03T

A-5.37E-03G

A-4.06E-03A

Sheet2

Sheet3

Evidencias para contrastar la Hiptesis de la Presin Termodinmica

EVOLUCIN IN VITRO POR INTERMEDIO DE PCR

Incremento de GC en el experimento de Briones

Chart1

0.4883616831

0.4892569382

0.4910313901

0.4914798206

0.4932735426

especies

%GC

T1

Hoja1

ACGTTotalP(A)P(C)P(G)P(T)%GC

56050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

56050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

55550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

55550459257922300.24887892380.22600896860.2654708520.25964125560.4914798206

55050459658022300.24663677130.22600896860.2672645740.26008968610.4932735426

&A

Pgina &P

Hoja1

&A

Page &P

especies

%GC

T1

Hoja2

ACGTtotalP(A)P(C)P(G)P(T)%GC

56050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

56050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

55550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

55550459257922300.24887892380.22600896860.2654708520.25964125560.4914798206

55350559357922300.24798206280.22645739910.26591928250.25964125560.4923766816

&A

Pgina &P

Hoja2

&A

Page &P

especie

%GC

T2

Hoja3

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.155550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

4.255450259358122300.24843049330.22511210760.26591928250.26053811660.4910313901

T 355550259258122300.24887892380.22511210760.2654708520.26053811660.4905829596

&A

Pgina &P

Hoja3

&A

Page &P

especies

%GC

T3

Hoja4

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.155550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

4.255450259358122300.24843049330.22511210760.26591928250.26053811660.4910313901

T455150359658022300.24708520180.22556053810.2672645740.26008968610.4928251121

&A

Pgina &P

Hoja4

&A

Page &P

especies

%GC

T4

Hoja5

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.355350259657822290.24809331540.22521310.26738447730.25930910720.4925975774

T555250259757822290.24764468370.22521310.2678331090.25930910720.4930462091

&A

Pgina &P

Hoja5

&A

Page &P

especies

%GC

T5

Hoja6

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.355350259657822290.24809331540.22521310.26738447730.25930910720.4925975774

T655150459857422270.24741805120.22631342610.26852267620.25774584640.4948361024

&A

Pgina &P

Hoja6

0.4883616831

0.4892569382

0.4901345291

0.4925975774

0.4948361024

&A

Page &P

especies

%GC

T6

Hoja7

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.455650259557722300.24932735430.22511210760.26681614350.25874439460.4919282511

T755450659657322290.24854194710.22700762670.26738447730.25706594890.4943921041

&A

Pgina &P

Hoja7

0.4883616831

0.4892569382

0.4901345291

0.4919282511

0.4943921041

&A

Page &P

especies

%GC

T7

Hoja8

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.455650259557722300.24932735430.22511210760.26681614350.25874439460.4919282511

T855750159657622300.24977578480.22466367710.2672645740.25829596410.4919282511

&A

Pgina &P

Hoja8

0.4883616831

0.4892569382

0.4901345291

0.4919282511

0.4919282511

&A

Page &P

especies

%GC

T8

Hoja9

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.555550059358322310.24876736890.22411474680.26580008960.26131779470.4899148364

T955849959158222300.25022421520.22376681610.26502242150.26098654710.4887892377

&A

Pgina &P

Hoja9

0.4883616831

0.4892569382

0.4901433692

0.4899148364

0.4887892377

&A

Page &P

especies

%GC

T8

Hoja10

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.555550059358322310.24876736890.22411474680.26580008960.26131779470.4899148364

T1055250059658322310.24742268040.22411474680.26714477810.26131779470.4912595249

&A

Pgina &P

Hoja10

0.4883616831

0.4892569382

0.4901433692

0.4899148364

0.4912595249

&A

Page &P

%GC

T10

Hoja11

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.655950259257822310.25056028690.22501120570.26535186020.25907664720.4903630659

T1155950359257622300.25067264570.22556053810.2654708520.25829596410.4910313901

&A

Pgina &P

Hoja11

0.4883616831

0.4892569382

0.4901433692

0.4903630659

0.4910313901

&A

Page &P

%GC

T11

Hoja12

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.655950259257822310.25056028690.22501120570.26535186020.25907664720.4903630659

T1255949959357922300.25067264570.22376681610.26591928250.25964125560.4896860987

&A

Pgina &P

Hoja12

0.4883616831

0.4892569382

0.4901433692

0.4903630659

0.4896860987

&A

Page &P

especies

%GC

T12

Hoja13

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1356350758757722340.25201432410.22694717990.26275738590.25828111010.4897045658

&A

Pgina &P

Hoja13

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4897045658

&A

Page &P

especies

%GC

t13

Hoja14

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1455950659057722320.25044802870.2267025090.26433691760.25851254480.4910394265

&A

Pgina &P

Hoja14

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4910394265

&A

Page &P

%GC

especies

%GC

T14

Hoja15

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1556350458558122330.25212718320.22570532920.26197939990.26018808780.4876847291

&A

Pgina &P

Hoja15

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4876847291

&A

Page &P

especies

%GC

T15

Hoja16

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1656450258558222330.25257501120.22480967310.26197939990.26063591580.486789073

&A

Pgina &P

Hoja16

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.486789073

&A

Page &P

especies

%GC

T16

Chart2

0.4883616831

0.4892569382

0.4910313901

0.4914798206

0.4923766816

especie

%GC

T2

Hoja1

ACGTTotalP(A)P(C)P(G)P(T)%GC

56050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

56050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

55550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

55550459257922300.24887892380.22600896860.2654708520.25964125560.4914798206

55050459658022300.24663677130.22600896860.2672645740.26008968610.4932735426

&A

Pgina &P

Hoja1

&A

Page &P

especies

%GC

T1

Hoja2

ACGTtotalP(A)P(C)P(G)P(T)%GC

56050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

56050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

55550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

55550459257922300.24887892380.22600896860.2654708520.25964125560.4914798206

55350559357922300.24798206280.22645739910.26591928250.25964125560.4923766816

&A

Pgina &P

Hoja2

&A

Page &P

especie

%GC

T2

Hoja3

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.155550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

4.255450259358122300.24843049330.22511210760.26591928250.26053811660.4910313901

T 355550259258122300.24887892380.22511210760.2654708520.26053811660.4905829596

&A

Pgina &P

Hoja3

&A

Page &P

especies

%GC

T3

Hoja4

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.155550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

4.255450259358122300.24843049330.22511210760.26591928250.26053811660.4910313901

T455150359658022300.24708520180.22556053810.2672645740.26008968610.4928251121

&A

Pgina &P

Hoja4

&A

Page &P

especies

%GC

T4

Hoja5

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.355350259657822290.24809331540.22521310.26738447730.25930910720.4925975774

T555250259757822290.24764468370.22521310.2678331090.25930910720.4930462091

&A

Pgina &P

Hoja5

&A

Page &P

especies

%GC

T5

Hoja6

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.355350259657822290.24809331540.22521310.26738447730.25930910720.4925975774

T655150459857422270.24741805120.22631342610.26852267620.25774584640.4948361024

&A

Pgina &P

Hoja6

0.4883616831

0.4892569382

0.4901345291

0.4925975774

0.4948361024

&A

Page &P

especies

%GC

T6

Hoja7

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.455650259557722300.24932735430.22511210760.26681614350.25874439460.4919282511

T755450659657322290.24854194710.22700762670.26738447730.25706594890.4943921041

&A

Pgina &P

Hoja7

0.4883616831

0.4892569382

0.4901345291

0.4919282511

0.4943921041

&A

Page &P

especies

%GC

T7

Hoja8

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.455650259557722300.24932735430.22511210760.26681614350.25874439460.4919282511

T855750159657622300.24977578480.22466367710.2672645740.25829596410.4919282511

&A

Pgina &P

Hoja8

0.4883616831

0.4892569382

0.4901345291

0.4919282511

0.4919282511

&A

Page &P

especies

%GC

T8

Hoja9

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.555550059358322310.24876736890.22411474680.26580008960.26131779470.4899148364

T955849959158222300.25022421520.22376681610.26502242150.26098654710.4887892377

&A

Pgina &P

Hoja9

0.4883616831

0.4892569382

0.4901433692

0.4899148364

0.4887892377

&A

Page &P

especies

%GC

T8

Hoja10

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.555550059358322310.24876736890.22411474680.26580008960.26131779470.4899148364

T1055250059658322310.24742268040.22411474680.26714477810.26131779470.4912595249

&A

Pgina &P

Hoja10

0.4883616831

0.4892569382

0.4901433692

0.4899148364

0.4912595249

&A

Page &P

%GC

T10

Hoja11

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.655950259257822310.25056028690.22501120570.26535186020.25907664720.4903630659

T1155950359257622300.25067264570.22556053810.2654708520.25829596410.4910313901

&A

Pgina &P

Hoja11

0.4883616831

0.4892569382

0.4901433692

0.4903630659

0.4910313901

&A

Page &P

%GC

T11

Hoja12

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.655950259257822310.25056028690.22501120570.26535186020.25907664720.4903630659

T1255949959357922300.25067264570.22376681610.26591928250.25964125560.4896860987

&A

Pgina &P

Hoja12

0.4883616831

0.4892569382

0.4901433692

0.4903630659

0.4896860987

&A

Page &P

especies

%GC

T12

Hoja13

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1356350758757722340.25201432410.22694717990.26275738590.25828111010.4897045658

&A

Pgina &P

Hoja13

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4897045658

&A

Page &P

especies

%GC

t13

Hoja14

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1455950659057722320.25044802870.2267025090.26433691760.25851254480.4910394265

&A

Pgina &P

Hoja14

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4910394265

&A

Page &P

%GC

especies

%GC

T14

Hoja15

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1556350458558122330.25212718320.22570532920.26197939990.26018808780.4876847291

&A

Pgina &P

Hoja15

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4876847291

&A

Page &P

especies

%GC

T15

Hoja16

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1656450258558222330.25257501120.22480967310.26197939990.26063591580.486789073

&A

Pgina &P

Hoja16

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.486789073

&A

Page &P

especies

%GC

T16

Chart3

0.4883616831

0.4892569382

0.4910313901

0.4910313901

0.4905829596

especies

%GC

T3

Hoja1

ACGTTotalP(A)P(C)P(G)P(T)%GC

56050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

56050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

55550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

55550459257922300.24887892380.22600896860.2654708520.25964125560.4914798206

55050459658022300.24663677130.22600896860.2672645740.26008968610.4932735426

&A

Pgina &P

Hoja1

&A

Page &P

especies

%GC

T1

Hoja2

ACGTtotalP(A)P(C)P(G)P(T)%GC

56050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

56050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

55550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

55550459257922300.24887892380.22600896860.2654708520.25964125560.4914798206

55350559357922300.24798206280.22645739910.26591928250.25964125560.4923766816

&A

Pgina &P

Hoja2

&A

Page &P

especie

%GC

T2

Hoja3

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.155550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

4.255450259358122300.24843049330.22511210760.26591928250.26053811660.4910313901

T 355550259258122300.24887892380.22511210760.2654708520.26053811660.4905829596

&A

Pgina &P

Hoja3

&A

Page &P

especies

%GC

T3

Hoja4

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.155550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

4.255450259358122300.24843049330.22511210760.26591928250.26053811660.4910313901

T455150359658022300.24708520180.22556053810.2672645740.26008968610.4928251121

&A

Pgina &P

Hoja4

&A

Page &P

especies

%GC

T4

Hoja5

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.355350259657822290.24809331540.22521310.26738447730.25930910720.4925975774

T555250259757822290.24764468370.22521310.2678331090.25930910720.4930462091

&A

Pgina &P

Hoja5

&A

Page &P

especies

%GC

T5

Hoja6

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.355350259657822290.24809331540.22521310.26738447730.25930910720.4925975774

T655150459857422270.24741805120.22631342610.26852267620.25774584640.4948361024

&A

Pgina &P

Hoja6

0.4883616831

0.4892569382

0.4901345291

0.4925975774

0.4948361024

&A

Page &P

especies

%GC

T6

Hoja7

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.455650259557722300.24932735430.22511210760.26681614350.25874439460.4919282511

T755450659657322290.24854194710.22700762670.26738447730.25706594890.4943921041

&A

Pgina &P

Hoja7

0.4883616831

0.4892569382

0.4901345291

0.4919282511

0.4943921041

&A

Page &P

especies

%GC

T7

Hoja8

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.455650259557722300.24932735430.22511210760.26681614350.25874439460.4919282511

T855750159657622300.24977578480.22466367710.2672645740.25829596410.4919282511

&A

Pgina &P

Hoja8

0.4883616831

0.4892569382

0.4901345291

0.4919282511

0.4919282511

&A

Page &P

especies

%GC

T8

Hoja9

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.555550059358322310.24876736890.22411474680.26580008960.26131779470.4899148364

T955849959158222300.25022421520.22376681610.26502242150.26098654710.4887892377

&A

Pgina &P

Hoja9

0.4883616831

0.4892569382

0.4901433692

0.4899148364

0.4887892377

&A

Page &P

especies

%GC

T8

Hoja10

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.555550059358322310.24876736890.22411474680.26580008960.26131779470.4899148364

T1055250059658322310.24742268040.22411474680.26714477810.26131779470.4912595249

&A

Pgina &P

Hoja10

0.4883616831

0.4892569382

0.4901433692

0.4899148364

0.4912595249

&A

Page &P

%GC

T10

Hoja11

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.655950259257822310.25056028690.22501120570.26535186020.25907664720.4903630659

T1155950359257622300.25067264570.22556053810.2654708520.25829596410.4910313901

&A

Pgina &P

Hoja11

0.4883616831

0.4892569382

0.4901433692

0.4903630659

0.4910313901

&A

Page &P

%GC

T11

Hoja12

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.655950259257822310.25056028690.22501120570.26535186020.25907664720.4903630659

T1255949959357922300.25067264570.22376681610.26591928250.25964125560.4896860987

&A

Pgina &P

Hoja12

0.4883616831

0.4892569382

0.4901433692

0.4903630659

0.4896860987

&A

Page &P

especies

%GC

T12

Hoja13

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1356350758757722340.25201432410.22694717990.26275738590.25828111010.4897045658

&A

Pgina &P

Hoja13

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4897045658

&A

Page &P

especies

%GC

t13

Hoja14

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1455950659057722320.25044802870.2267025090.26433691760.25851254480.4910394265

&A

Pgina &P

Hoja14

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4910394265

&A

Page &P

%GC

especies

%GC

T14

Hoja15

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1556350458558122330.25212718320.22570532920.26197939990.26018808780.4876847291

&A

Pgina &P

Hoja15

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4876847291

&A

Page &P

especies

%GC

T15

Hoja16

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1656450258558222330.25257501120.22480967310.26197939990.26063591580.486789073

&A

Pgina &P

Hoja16

0.4883616831

0.4892569382

0.4901433692

0.4883616831

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&A

Page &P

especies

%GC

T16

Chart4

0.4883616831

0.4892569382

0.4910313901

0.4910313901

0.4928251121

especies

%GC

T4

Hoja1

ACGTTotalP(A)P(C)P(G)P(T)%GC

56050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

56050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

55550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

55550459257922300.24887892380.22600896860.2654708520.25964125560.4914798206

55050459658022300.24663677130.22600896860.2672645740.26008968610.4932735426

&A

Pgina &P

Hoja1

&A

Page &P

especies

%GC

T1

Hoja2

ACGTtotalP(A)P(C)P(G)P(T)%GC

56050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

56050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

55550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

55550459257922300.24887892380.22600896860.2654708520.25964125560.4914798206

55350559357922300.24798206280.22645739910.26591928250.25964125560.4923766816

&A

Pgina &P

Hoja2

&A

Page &P

especie

%GC

T2

Hoja3

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.155550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

4.255450259358122300.24843049330.22511210760.26591928250.26053811660.4910313901

T 355550259258122300.24887892380.22511210760.2654708520.26053811660.4905829596

&A

Pgina &P

Hoja3

&A

Page &P

especies

%GC

T3

Hoja4

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.155550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

4.255450259358122300.24843049330.22511210760.26591928250.26053811660.4910313901

T455150359658022300.24708520180.22556053810.2672645740.26008968610.4928251121

&A

Pgina &P

Hoja4

&A

Page &P

especies

%GC

T4

Hoja5

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.355350259657822290.24809331540.22521310.26738447730.25930910720.4925975774

T555250259757822290.24764468370.22521310.2678331090.25930910720.4930462091

&A

Pgina &P

Hoja5

&A

Page &P

especies

%GC

T5

Hoja6

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.355350259657822290.24809331540.22521310.26738447730.25930910720.4925975774

T655150459857422270.24741805120.22631342610.26852267620.25774584640.4948361024

&A

Pgina &P

Hoja6

0.4883616831

0.4892569382

0.4901345291

0.4925975774

0.4948361024

&A

Page &P

especies

%GC

T6

Hoja7

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.455650259557722300.24932735430.22511210760.26681614350.25874439460.4919282511

T755450659657322290.24854194710.22700762670.26738447730.25706594890.4943921041

&A

Pgina &P

Hoja7

0.4883616831

0.4892569382

0.4901345291

0.4919282511

0.4943921041

&A

Page &P

especies

%GC

T7

Hoja8

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.455650259557722300.24932735430.22511210760.26681614350.25874439460.4919282511

T855750159657622300.24977578480.22466367710.2672645740.25829596410.4919282511

&A

Pgina &P

Hoja8

0.4883616831

0.4892569382

0.4901345291

0.4919282511

0.4919282511

&A

Page &P

especies

%GC

T8

Hoja9

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.555550059358322310.24876736890.22411474680.26580008960.26131779470.4899148364

T955849959158222300.25022421520.22376681610.26502242150.26098654710.4887892377

&A

Pgina &P

Hoja9

0.4883616831

0.4892569382

0.4901433692

0.4899148364

0.4887892377

&A

Page &P

especies

%GC

T8

Hoja10

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.555550059358322310.24876736890.22411474680.26580008960.26131779470.4899148364

T1055250059658322310.24742268040.22411474680.26714477810.26131779470.4912595249

&A

Pgina &P

Hoja10

0.4883616831

0.4892569382

0.4901433692

0.4899148364

0.4912595249

&A

Page &P

%GC

T10

Hoja11

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.655950259257822310.25056028690.22501120570.26535186020.25907664720.4903630659

T1155950359257622300.25067264570.22556053810.2654708520.25829596410.4910313901

&A

Pgina &P

Hoja11

0.4883616831

0.4892569382

0.4901433692

0.4903630659

0.4910313901

&A

Page &P

%GC

T11

Hoja12

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.655950259257822310.25056028690.22501120570.26535186020.25907664720.4903630659

T1255949959357922300.25067264570.22376681610.26591928250.25964125560.4896860987

&A

Pgina &P

Hoja12

0.4883616831

0.4892569382

0.4901433692

0.4903630659

0.4896860987

&A

Page &P

especies

%GC

T12

Hoja13

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1356350758757722340.25201432410.22694717990.26275738590.25828111010.4897045658

&A

Pgina &P

Hoja13

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4897045658

&A

Page &P

especies

%GC

t13

Hoja14

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1455950659057722320.25044802870.2267025090.26433691760.25851254480.4910394265

&A

Pgina &P

Hoja14

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4910394265

&A

Page &P

%GC

especies

%GC

T14

Hoja15

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1556350458558122330.25212718320.22570532920.26197939990.26018808780.4876847291

&A

Pgina &P

Hoja15

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4876847291

&A

Page &P

especies

%GC

T15

Hoja16

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1656450258558222330.25257501120.22480967310.26197939990.26063591580.486789073

&A

Pgina &P

Hoja16

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.486789073

&A

Page &P

especies

%GC

T16

Chart5

0.4883616831

0.4892569382

0.4901345291

0.4925975774

0.4930462091

especies

%GC

T5

Hoja1

ACGTTotalP(A)P(C)P(G)P(T)%GC

56050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

56050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

55550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

55550459257922300.24887892380.22600896860.2654708520.25964125560.4914798206

55050459658022300.24663677130.22600896860.2672645740.26008968610.4932735426

&A

Pgina &P

Hoja1

&A

Page &P

especies

%GC

T1

Hoja2

ACGTtotalP(A)P(C)P(G)P(T)%GC

56050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

56050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

55550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

55550459257922300.24887892380.22600896860.2654708520.25964125560.4914798206

55350559357922300.24798206280.22645739910.26591928250.25964125560.4923766816

&A

Pgina &P

Hoja2

&A

Page &P

especie

%GC

T2

Hoja3

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.155550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

4.255450259358122300.24843049330.22511210760.26591928250.26053811660.4910313901

T 355550259258122300.24887892380.22511210760.2654708520.26053811660.4905829596

&A

Pgina &P

Hoja3

&A

Page &P

especies

%GC

T3

Hoja4

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.155550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

4.255450259358122300.24843049330.22511210760.26591928250.26053811660.4910313901

T455150359658022300.24708520180.22556053810.2672645740.26008968610.4928251121

&A

Pgina &P

Hoja4

&A

Page &P

especies

%GC

T4

Hoja5

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.355350259657822290.24809331540.22521310.26738447730.25930910720.4925975774

T555250259757822290.24764468370.22521310.2678331090.25930910720.4930462091

&A

Pgina &P

Hoja5

&A

Page &P

especies

%GC

T5

Hoja6

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.355350259657822290.24809331540.22521310.26738447730.25930910720.4925975774

T655150459857422270.24741805120.22631342610.26852267620.25774584640.4948361024

&A

Pgina &P

Hoja6

0.4883616831

0.4892569382

0.4901345291

0.4925975774

0.4948361024

&A

Page &P

especies

%GC

T6

Hoja7

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.455650259557722300.24932735430.22511210760.26681614350.25874439460.4919282511

T755450659657322290.24854194710.22700762670.26738447730.25706594890.4943921041

&A

Pgina &P

Hoja7

0.4883616831

0.4892569382

0.4901345291

0.4919282511

0.4943921041

&A

Page &P

especies

%GC

T7

Hoja8

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.455650259557722300.24932735430.22511210760.26681614350.25874439460.4919282511

T855750159657622300.24977578480.22466367710.2672645740.25829596410.4919282511

&A

Pgina &P

Hoja8

0.4883616831

0.4892569382

0.4901345291

0.4919282511

0.4919282511

&A

Page &P

especies

%GC

T8

Hoja9

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.555550059358322310.24876736890.22411474680.26580008960.26131779470.4899148364

T955849959158222300.25022421520.22376681610.26502242150.26098654710.4887892377

&A

Pgina &P

Hoja9

0.4883616831

0.4892569382

0.4901433692

0.4899148364

0.4887892377

&A

Page &P

especies

%GC

T8

Hoja10

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.555550059358322310.24876736890.22411474680.26580008960.26131779470.4899148364

T1055250059658322310.24742268040.22411474680.26714477810.26131779470.4912595249

&A

Pgina &P

Hoja10

0.4883616831

0.4892569382

0.4901433692

0.4899148364

0.4912595249

&A

Page &P

%GC

T10

Hoja11

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.655950259257822310.25056028690.22501120570.26535186020.25907664720.4903630659

T1155950359257622300.25067264570.22556053810.2654708520.25829596410.4910313901

&A

Pgina &P

Hoja11

0.4883616831

0.4892569382

0.4901433692

0.4903630659

0.4910313901

&A

Page &P

%GC

T11

Hoja12

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.655950259257822310.25056028690.22501120570.26535186020.25907664720.4903630659

T1255949959357922300.25067264570.22376681610.26591928250.25964125560.4896860987

&A

Pgina &P

Hoja12

0.4883616831

0.4892569382

0.4901433692

0.4903630659

0.4896860987

&A

Page &P

especies

%GC

T12

Hoja13

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1356350758757722340.25201432410.22694717990.26275738590.25828111010.4897045658

&A

Pgina &P

Hoja13

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4897045658

&A

Page &P

especies

%GC

t13

Hoja14

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1455950659057722320.25044802870.2267025090.26433691760.25851254480.4910394265

&A

Pgina &P

Hoja14

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4910394265

&A

Page &P

%GC

especies

%GC

T14

Hoja15

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1556350458558122330.25212718320.22570532920.26197939990.26018808780.4876847291

&A

Pgina &P

Hoja15

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4876847291

&A

Page &P

especies

%GC

T15

Hoja16

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1656450258558222330.25257501120.22480967310.26197939990.26063591580.486789073

&A

Pgina &P

Hoja16

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.486789073

&A

Page &P

especies

%GC

T16

Chart6

0.4883616831

0.4892569382

0.4901433692

0.4903630659

0.4910313901

%GC

T11

Hoja1

ACGTTotalP(A)P(C)P(G)P(T)%GC

56050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

56050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

55550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

55550459257922300.24887892380.22600896860.2654708520.25964125560.4914798206

55050459658022300.24663677130.22600896860.2672645740.26008968610.4932735426

&A

Pgina &P

Hoja1

&A

Page &P

especies

%GC

T1

Hoja2

ACGTtotalP(A)P(C)P(G)P(T)%GC

56050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

56050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

55550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

55550459257922300.24887892380.22600896860.2654708520.25964125560.4914798206

55350559357922300.24798206280.22645739910.26591928250.25964125560.4923766816

&A

Pgina &P

Hoja2

&A

Page &P

especie

%GC

T2

Hoja3

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.155550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

4.255450259358122300.24843049330.22511210760.26591928250.26053811660.4910313901

T 355550259258122300.24887892380.22511210760.2654708520.26053811660.4905829596

&A

Pgina &P

Hoja3

&A

Page &P

especies

%GC

T3

Hoja4

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.155550359258022300.24887892380.22556053810.2654708520.26008968610.4910313901

4.255450259358122300.24843049330.22511210760.26591928250.26053811660.4910313901

T455150359658022300.24708520180.22556053810.2672645740.26008968610.4928251121

&A

Pgina &P

Hoja4

&A

Page &P

especies

%GC

T4

Hoja5

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.355350259657822290.24809331540.22521310.26738447730.25930910720.4925975774

T555250259757822290.24764468370.22521310.2678331090.25930910720.4930462091

&A

Pgina &P

Hoja5

&A

Page &P

especies

%GC

T5

Hoja6

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.355350259657822290.24809331540.22521310.26738447730.25930910720.4925975774

T655150459857422270.24741805120.22631342610.26852267620.25774584640.4948361024

&A

Pgina &P

Hoja6

&A

Page &P

especies

%GC

T6

Hoja7

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.455650259557722300.24932735430.22511210760.26681614350.25874439460.4919282511

T755450659657322290.24854194710.22700762670.26738447730.25706594890.4943921041

&A

Pgina &P

Hoja7

&A

Page &P

especies

%GC

T7

Hoja8

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.156050359058122340.25067144140.22515666970.26410026860.26007162040.4892569382

3.255650159258122300.24932735430.22466367710.2654708520.26053811660.4901345291

4.455650259557722300.24932735430.22511210760.26681614350.25874439460.4919282511

T855750159657622300.24977578480.22466367710.2672645740.25829596410.4919282511

&A

Pgina &P

Hoja8

&A

Page &P

especies

%GC

T8

Hoja9

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.555550059358322310.24876736890.22411474680.26580008960.26131779470.4899148364

T955849959158222300.25022421520.22376681610.26502242150.26098654710.4887892377

&A

Pgina &P

Hoja9

&A

Page &P

especies

%GC

T8

Hoja10

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.555550059358322310.24876736890.22411474680.26580008960.26131779470.4899148364

T1055250059658322310.24742268040.22411474680.26714477810.26131779470.4912595249

&A

Pgina &P

Hoja10

&A

Page &P

%GC

T10

Hoja11

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.655950259257822310.25056028690.22501120570.26535186020.25907664720.4903630659

T1155950359257622300.25067264570.22556053810.2654708520.25829596410.4910313901

&A

Pgina &P

Hoja11

&A

Page &P

%GC

T11

Hoja12

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.655950259257822310.25056028690.22501120570.26535186020.25907664720.4903630659

T1255949959357922300.25067264570.22376681610.26591928250.25964125560.4896860987

&A

Pgina &P

Hoja12

0.4883616831

0.4892569382

0.4901433692

0.4903630659

0.4896860987

&A

Page &P

especies

%GC

T12

Hoja13

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1356350758757722340.25201432410.22694717990.26275738590.25828111010.4897045658

&A

Pgina &P

Hoja13

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4897045658

&A

Page &P

especies

%GC

t13

Hoja14

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1455950659057722320.25044802870.2267025090.26433691760.25851254480.4910394265

&A

Pgina &P

Hoja14

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4910394265

&A

Page &P

%GC

especies

%GC

T14

Hoja15

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1556350458558122330.25212718320.22570532920.26197939990.26018808780.4876847291

&A

Pgina &P

Hoja15

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.4876847291

&A

Page &P

especies

%GC

T15

Hoja16

ACGTtotalP(A)P(C)P(G)P(T)%GC

1.156050159058322340.25067144140.22426141450.26410026860.26096687560.4883616831

2.255850159258322340.24977618620.22426141450.26499552370.26096687560.4892569382

3.355850159358022320.250.22446236560.26568100360.25985663080.4901433692

4.756350358858022340.25201432410.22515666970.26320501340.25962399280.4883616831

T1656450258558222330.25257501120.22480967310.26197939990.26063591580.486789073

&A

Pgina &P

Hoja16

0.4883616831

0.4892569382

0.4901433692

0.4883616831

0.486789073

&A

Page &P

especies

%GC

T16

Evolucin in-vitro por PCR en fro aumentar el %GC ?Los seleccionistas sostienen que el incremento del %GC observado en el experimento de Briones se debera a que la taq polimerasa es una isoforma adaptada a condiciones de altas temperaturas y de manera natural prefiere acumular bases G y C

Variacin del %GC para cada posicin de codon reportado en Trypanosomatidos(Alonso et al,1992)

_977103701.doc

42

44

46

48

50

52

54

56

58

60

40

45

50

55

60

65

70

75

80

85

90

Crithidia

Leishmania

T.cruzi

T.brucei

1st

2nd

3rd

% GC total DNA

_977103911.doc

42

44

46

48

50

52

54

56

58

60

40

45

50

55

60

65

70

75

80

85

90

Crithidia

Leishmania

T.cruzi

T.brucei

1st

2nd

3rd

%GC

codon

position

% GC total DNA

_977103235.doc

42

44

46

48

50

52

54

56

58

60

40

45

50

55

60

65

70

75

80

85

90

Crithidia

Leishmania

T.cruzi

T.brucei

B 1ra

C 2da

D 3ra

Contenido GC en codn (%)

% GC total DNA

Usaje de Codones en kinetoplastida leucine

Chart4

5.3

7.8

15.3

25.5

23.6

22.3

2.3

4

10.8

22.3

16.6

44

0.7

2.5

4.8

12.1

18.5

61.3

0.6

1.9

3.8

5.7

22.3

65.6

T.brucei T.cruzi Leishmania Critidia

Hoja1

CritidiaLeishmaniaT. CruziT. Brucei

PheTTT8.3155245.3

TTC91.6854854.7

LeuTTA0.60.72.35.3

TTG3.84.810.815.3

CTT5.712.122.325.5

CTC22.318.516.623.6

CTA1.92.547.8

CTG65.661.34422.3

IleATT21.121.444.244.1

ATC78.876.947.742.5

ATA01.6813.3

MetATG100100100100

ValGTT6.49.231.529.5

GTC16.52016.616.9

GTA0.74.23.713.1

GTG76.366.648.240.3

SerTCT4.86.39.817.1

TCC19.418.916.621.8

TCA2.95.38.714.4

TCG35.933.418.813

AGT1.95.41915

AGC34.928.727.118.6

ProCCT6.215.510.521.5

CCC13.415.841.830.5

CCA9.39.414.227.2

CCG71.159.233.520.7

ThrACT7.47.618.820

ACC23.419.617.525

ACA3.75.112.925.5

ACG65.467.650.829.5

AlaGCT15.815.525.427.5

GCC26.725.228.826.1

GCA7.18.621.324.5

GCG50.350.724.421.8

TyrTAT5.77.814.835.1

TAC94.292.28564.9

stopTAA2516.612.546.2

TAG5066.643.730.8

TGA2516.643.723

HisCAT3.38.91536.2

CAC96.691.18563.8

GlnCAA6.64.51837.3

CAG93.395.48262.6

AsnAAT1.87.43738.2

AAC98.292.66361.7

LysAAA1.77.22031.7

AAG98.392.88068.2

AspGAT10.720.72748.4

GAC89.379.37351.6

GluGAA3.77.83439.7

GAG96.392.26660.2

CysTGT3.71326.640.8

TGC96.38773.359.1

TrpTGG100100100100

ArgCGT1613.617.726.3

CCC5858.24333

CGA4.948.711.5

CGG14.815.513.513.4

AGA2.51.25.45.7

AGG3.77.411.610.1

GlyGGT16.315.626.842.2

GGC65.266.243.527.8

GGA0.73.514.517

GGG17.714.615.212.9

Hoja2

CGT26.3

CCC33

CGA11.5

CGG13.4

AGA5.7

AGG10.1

CGT17.7

CCC43

CGA8.7

CGG13.5

AGA5.4

AGG11.6

CGT13.6

CCC58.2

CGA4

CGG15.5

AGA1.2

AGG7.4

CGT16

CCC58

CGA4.9

CGG14.8TAT35.1

AGA2.5TAC64.9

AGG3.7

TyrTAT5.77.814.835.1TAT14.8

TAC94.292.28564.9TAC85

TAT7.8

TAC92.2

TAT5.7

TAC94.2

ACT20

ACC25

ThrACT7.47.618.820ACA25.5

ACC23.419.617.525ACG29.5

ACA3.75.112.925.5

ACG65.467.650.829.5ACT18.8

ACC17.5

ACA12.9

ACG50.8

ACT7.6

ACC19.6

ACA5.1

ACG67.6

ACT7.4

ACC23.4

ACA3.7

ACG65.4

GCT27.5

GCA24.5

GCC26.1

AlaGCT15.815.525.427.5GCG21.8

GCC26.725.228.826.1

GCA7.18.621.324.5GCT25.4

GCG50.350.724.421.8GCA21.3

GCC28.8

GCG24.4

GCT15.5

GCA8.6

GCC25.2

GCG50.7

GCT15.8

GCA7.1

GCC26.7

GCG50.3

GGT42.2

GGA17

GlyGGT16.315.626.842.2GGC27.8

GGA0.73.514.517GGG12.9

GGC65.266.243.527.8

GGG17.714.615.212.9GGT26.8

GGA14.5

GGC43.5

GGG15.2

GGT15.6

GGA3.5

GGC66.2

GGG14.6

GGT16.3

GGA0.7

GGC65.2

GGG17.7

GTT29.5

GTA13.1

ValGTT6.49.231.529.5GTC16.9

GTA0.74.23.713.1GTG40.3

GTC16.52016.616.9

GTG76.366.648.240.3GTT31.5

GTA3.7

GTC16.6

GTG48.2

GTT9.2

GTA4.2

GTC20

GTG66.6

GTT6.4

GTA0.7

GTC16.5

GTG76.3

CCT21.5

CCA27.2

ProCCT6.215.510.521.5CCC30.5

CCA9.39.414.227.2CCG20.7

CCC13.415.841.830.5

CCG71.159.233.520.7CCT10.5

CCA14.2

CCC41.8

CCG33.5

CCT15.5

CCA9.4

CCC15.8

CCG59.2

CCT6.2

CCA9.3

CCC13.4

CCG71.1

ATT44.1

ATC42.5

ATA13.3

IleATT21.121.444.244.1ATT44.2

ATC78.876.947.742.5ATC47.7

ATA01.6813.3ATA8

ATT21.4

ATC76.9

ATA1.6

ATT21.1

ATC78.8

ATA0

TTA5.3

CTA7.8

TTG15.3

LeuTTA0.60.72.35.3CTT25.5

CTA1.92.547.8CTC23.6

TTG3.84.810.815.3CTG22.3

CTT5.712.122.325.5

CTC22.318.516.623.6TTA2.3

CTG65.661.34422.3CTA4

TTG10.8

CTT22.3

CTC16.6

CTG44

TTA0.7

CTA2.5

TTG4.8

CTT12.1

CTC18.5

CTG61.3

TTA0.6

CTA1.9

TTG3.8

CTT5.7

CTC22.3

CTG65.6

SerAGT1.95.41915AGT15

TCA2.95.38.714.4TCA14.4

TCT4.86.39.817.1TCT17.1

TCC19.418.916.621.8TCC21.8

AGC34.928.727.118.6AGC18.6

TCG35.933.418.813TCG13

AGT19

TCA8.7

TCT9.8

TCC16.6

AGC27.1

TCG18.8

AGT5.4

TCA5.3

TCT6.3

TCC18.9

AGC28.7

TCG33.4

AGT1.9

TCA2.9

TCT4.8

TCC19.4

AGC34.9

TCG35.9

Hoja2

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

T.brucei T.cruzi Leishmania Critidia

Tyrosina

Hoja3

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

T.brucei T.cruzi Leishmania Critidia

Arginina

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

T.brucei T.cruzi Leishmania Critidia

Treonina

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

T.brucei T.cruzi Leishmania Critidia

Alanina

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

T.brucei T.cruzi Leishmania Critidia

Glicinia

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

T.brucei T.cruzi Leishmania Critidia

Valina

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

T.brucei T.cruzi Leishmania Critidia

Prolina

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

T.brucei T.cruzi Leishmania Critidia

Isoleucina

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

T.brucei T.cruzi Leishmania Critidia

Leucina

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

T.brucei T.cruzi Leishmania Critidia

Serina

Filogenia del linaje de kinetoplastida (Maslov & Simpson) 0 Euglena 1 T.borelli or Bodo 2 T.brucei 3 T.cruzi or T. sp 4 Blastocrithidia 5 Phytomona or Herpetomona 6 Leishmania or Endotripanosoma 7 Crithidia or Leptomona

Reconstruccin de los ancestros extintos (nodos), utilizando el gen GPDH

/----------------------------------------------- T.brucei/-------14| \------------------------------ T.cruzi|| /-------- Leptomonas| /-----9| | \-------- Crithidia| /------------1013 | \---------------- L.mexinana| /-----------11| | \------------------ Herpetomonas+-------------------12| \------------------- Phytomonas|\--------------------------------------------- T.borreli

Chart7

56.3323201621

60.5092091008

63.880126183

65.5605832076

%GC

%GC Total

Trypanosomatidae: HSP70

GC total

TRYPANOSOMATIDOS

1. GENES NUCLEARES CODANTES

Cdigo%GC TOTAL%GC1%GC2%GC3%GC3*TOTALTOTAL GC3*# GENESHOSPEDERO

T. borreliTbo49.694155.924540.282052.898845.0355506725384Pez-leechDigentico

T. bruceiTbr50.698357.405440.701353.950646.633617113846614HumanoDigentico

T. cruziTcr58.394460.397342.052572.410371.612312693653113HumanoDigentico

Phytomonas sp.Phy57.077059.650443.044468.536064.9551411923375PlantaDigentico

Herpetomonas sp.Her63.241657.064242.752389.908386.092716359062InsectoMonogentico

Leishmania sp.Lei61.937459.884342.738284.198481.7657440622245536HumanoDigentico

Leptomonas sp.Lep61.832857.103940.965487.429286.174914295744312InsectoMonogentico

C.fasciculataCfa63.735160.118442.131788.912686.470720262953116InsectoMonogentico

0

NOTA: En estos cuadros no se incluye a Herpetomonas ya que tiene slo

dos secuencias analizadas. Se podra mencionar que se analizo en

discusin de resultados y que an as el modelo estadisticamente se ajusta.

PLASMODIUM

1. GENES NUCLEARES CODANTESHOSPEDEROHOSPEDERO

Cdigo%GC%GC1%GC2%GC3%GC3*TOTALTOTAL GC3*# GENESINTERMEDIARIODEFINITIVO

P. falciparumPfa28.326739.330527.897618.222214.8981440681266631HumanoMosquito

P. reichenowiPre28.815141.445230.577714.418711.728720871636913Chimpance

P. gallinaceumPga31.663744.539734.927815.523513.7885664822416Mosquito

P. malariePma33.221644.321936.277618.967113.4616417815605HumanoMosquito

P. bergheiPbe31.662841.874535.057118.056714.348224423871516RoedoresMosquito

P. fragilePfr38.469347.383740.717227.159920.5047492619026

P. knowlesiPkn41.197047.748637.179538.655134.278823191867314

P. cynomolgiPcy41.108048.020837.109037.178032.004414440541810

P. vivaxPvi42.874147.681935.662445.278041.827822713853515HumanoMosquito

GC total

%GC

Kinetoplatida: %GC Total

Trypas_Homologos

%GC1

%GC2

%GC

Kinetoplastida: %GC1 & &GC2

Plas_Homlogos

%GC3

%GC3*

%GC

Kinetoplastida: %GC3 & %GC3*

%GC

%GC

Plasmodium: %GC Total

%GC1

%GC2

%GC

Plasmodium: %GC1 & %GC2

%GC3

%GC3*

%GC

Plasmodium: %GC3 & %GC3*

Trypanosomatidos

GENES NUCLEARES

HSP70CdigoTOTAL nucl.%GC%GC1%GC2%GC3%GC3*TOTAL GC3*

T. bruceiTbr197456.332360.242840.667767.830058.89211029

T. cruziTcr184660.509259.481438.087583.306380.7818921

L. majorLma190263.880158.914740.503993.217190.8759822

C. fasciculataCfa198965.560661.689340.874894.117689.53491032

HISTONE H1CdigoTOTAL nucl.%GC%GC1%GC2%GC3%GC3*TOTAL GC3*

T. bruceiTbr25554.509847.058857.647158.823544.4444135

T. cruziTcr29153.608248.453646.391865.979452.0000150

L. majorLma33661.904851.785759.821474.107156.0606198

C. fasciculataCfa45373.509963.576265.562991.390787.7358318

G3PDHCdigoTOTAL nucl.%GC%GC1%GC2%GC3%GC3*TOTAL GC3*

T. borreliTbo108949.035854.545541.046851.515244.4444567

T. bruceiTbr108056.481556.824543.175569.916462.3116597

T. cruziTcr107759.888657.660243.454078.551575.6345591

Phytomonas sp.Phy108359.187454.016641.551281.994576.4706561

Herpetomonas sp.Her108361.311253.739640.720289.473783.8542576

Leishmania sp.Lei108361.403555.401742.382386.426682.8125576

Leptomonas sp.Lep108364.542956.509741.828395.290993.4010591

C. fasciculataCfa108365.374055.955742.659397.506996.4467591

%GC

%GC Total

Trypanosomatidae: HSP70

%GC1

%GC2

%GC

Trypanosomatidae: HSP70

%GC3

%GC3*

%GC

Trypanosomatidae: HSP70

%GC

%GC Total

Trypanosomatidae: Histona H1

%GC1

%GC2

%GC

Trypanosomatidae: Histana H1

%GC3

%GC3*

%GC

Trypanosomatidae: Histona H1

%GC

%GC Total

Trypanosomatidae: G3PDH

%GC1

%GC2

%GC

Trypanosomatidae: G3PDH

%GC3

%GC3*

%GC

Trypanosomatidae: G3PDH

Plasmodium1/8/02

GENES NUCLEARES

CG10CdigoTOTAL%GC%GC1%GC2%GC12%GC3%GC3*TOTAL GC3*

P. falciparumPfa127228.380532.783030.896231.839621.462317.6923390

P.bergheiPbe127527.764729.176530.823530.000023.294120.6107393

P. knowlesiPkn127339.748638.679230.660434.669850.000054.1935465

P. vivaxPvi127245.283039.150931.603835.377465.094369.8718468

Chloroquine resistance transporter, para falciparum es putativo pero de muy alta homologa.

CDC2CdigoTOTAL%GC%GC1%GC2%GC12%GC3%GC3*TOTAL GC3*

P. falciparumPfa86433.333345.833332.291739.062521.875018.0952315

P.bergheiPbe86433.796345.486133.333339.409722.569425.0000300

P. knowlesiPkn86443.981550.000033.680641.840348.263956.2500336

P. vivaxPvi86444.791749.652832.638941.145852.083360.9091330

CDC2 protein kinase

CPCdigoTOTAL%GC%GC1%GC2%GC12%GC3%GC3*TOTAL GC3*

P. falciparumPfa170723.960234.446422.144128.295315.29009.5238378

P. reichenowiPre99324.068536.858024.773430.815710.57404.8780246

P. gallinaceumPga57026.315837.368429.473733.421112.10538.1633147

P. malariePma51931.984642.196534.682138.439319.075116.9492177

P. bergheiPbe98729.280639.209729.483334.346519.148916.3043276

P. fragilePfr99036.767741.515230.303035.909138.484838.0000300

P. cynomolgiPcy99636.747040.361431.626535.994038.253039.4231312

P. vivaxPvi174941.566644.425431.560937.993148.713651.2953579

%GC

%GC Total

Plasmodium: CG10

%GC1

%GC2

%GC

Plasmodium: CG10

%GC3

%GC3*

%GC

Plasmodium: CG10

%GC

%GC Total

Plasmodium: CDC2

%GC1

%GC2

%GC

Plasmodium: CDC2

%GC3

%GC3*

%GC

Plasmodium: CDC2

%GC

%GC Total

Plasmodium: Cistein Protease

%GC1

%GC2

%GC

Plasmodium: Cistein Protease

%GC3

%GC3*

%GC

Plasmodium: Cistein Protease

Chart8

60.242840.6677

59.481438.0875

58.914740.5039

61.689340.8748

%GC1

%GC2

%GC

Trypanosomatidae: HSP70

GC total

TRYPANOSOMATIDOS

1. GENES NUCLEARES CODANTES

Cdigo%GC TOTAL%GC1%GC2%GC3%GC3*TOTALTOTAL GC3*# GENESHOSPEDERO

T. borreliTbo49.694155.924540.282052.898845.0355506725384Pez-leechDigentico

T. bruceiTbr50.698357.405440.701353.950646.633617113846614HumanoDigentico

T. cruziTcr58.394460.397342.052572.410371.612312693653113HumanoDigentico

Phytomonas sp.Phy57.077059.650443.044468.536064.9551411923375PlantaDigentico

Herpetomonas sp.Her63.241657.064242.752389.908386.092716359062InsectoMonogentico

Leishmania sp.Lei61.937459.884342.738284.198481.7657440622245536HumanoDigentico

Leptomonas sp.Lep61.832857.103940.965487.429286.174914295744312InsectoMonogentico

C.fasciculataCfa63.735160.118442.131788.912686.470720262953116InsectoMonogentico

0

NOTA: En estos cuadros no se incluye a Herpetomonas ya que tiene slo

dos secuencias analizadas. Se podra mencionar que se analizo en

discusin de resultados y que an as el modelo estadisticamente se ajusta.

PLASMODIUM

1. GENES NUCLEARES CODANTESHOSPEDEROHOSPEDERO

Cdigo%GC%GC1%GC2%GC3%GC3*TOTALTOTAL GC3*# GENESINTERMEDIARIODEFINITIVO

P. falciparumPfa28.326739.330527.897618.222214.8981440681266631HumanoMosquito

P. reichenowiPre28.815141.445230.577714.418711.728720871636913Chimpance

P. gallinaceumPga31.663744.539734.927815.523513.7885664822416Mosquito

P. malariePma33.221644.321936.277618.967113.4616417815605HumanoMosquito

P. bergheiPbe31.662841.874535.057118.056714.348224423871516RoedoresMosquito

P. fragilePfr38.469347.383740.717227.159920.5047492619026

P. knowlesiPkn41.197047.748637.179538.655134.278823191867314

P. cynomolgiPcy41.108048.020837.109037.178032.004414440541810

P. vivaxPvi42.874147.681935.662445.278041.827822713853515HumanoMosquito

GC total

%GC

Kinetoplatida: %GC Total

Trypas_Homologos

%GC1

%GC2

%GC

Kinetoplastida: %GC1 & &GC2

Plas_Homlogos

%GC3

%GC3*

%GC

Kinetoplastida: %GC3 & %GC3*

%GC

%GC

Plasmodium: %GC Total

%GC1

%GC2

%GC

Plasmodium: %GC1 & %GC2

%GC3

%GC3*

%GC

Plasmodium: %GC3 & %GC3*

Trypanosomatidos

GENES NUCLEARES

HSP70CdigoTOTAL nucl.%GC%GC1%GC2%GC3%GC3*TOTAL GC3*

T. bruceiTbr197456.332360.242840.667767.830058.89211029

T. cruziTcr184660.509259.481438.087583.306380.7818921

L. majorLma190263.880158.914740.503993.217190.8759822

C. fasciculataCfa198965.560661.689340.874894.117689.53491032

HISTONE H1CdigoTOTAL nucl.%GC%GC1%GC2%GC3%GC3*TOTAL GC3*

T. bruceiTbr25554.509847.058857.647158.823544.4444135

T. cruziTcr29153.608248.453646.391865.979452.0000150

L. majorLma33661.904851.785759.821474.107156.0606198

C. fasciculataCfa45373.509963.576265.562991.390787.7358318

G3PDHCdigoTOTAL nucl.%GC%GC1%GC2%GC3%GC3*TOTAL GC3*

T. borreliTbo108949.035854.545541.046851.515244.4444567

T. bruceiTbr108056.481556.824543.175569.916462.3116597

T. cruziTcr107759.888657.660243.454078.551575.6345591

Phytomonas sp.Phy108359.187454.016641.551281.994576.4706561

Herpetomonas sp.Her108361.311253.739640.720289.473783.8542576

Leishmania sp.Lei108361.403555.401742.382386.426682.8125576

Leptomonas sp.Lep108364.542956.509741.828395.290993.4010591

C. fasciculataCfa108365.374055.955742.659397.506996.4467591

%GC

%GC Total

Trypanosomatidae: HSP70

%GC1

%GC2

%GC

Trypanosomatidae: HSP70

%GC3

%GC3*

%GC

Trypanosomatidae: HSP70

%GC

%GC Total

Trypanosomatidae: Histona H1

%GC1

%GC2

%GC

Trypanosomatidae: Histana H1

%GC3

%GC3*

%GC

Trypanosomatidae: Histona H1

%GC

%GC Total

Trypanosomatidae: G3PDH

%GC1

%GC2

%GC

Trypanosomatidae: G3PDH

%GC3

%GC3*

%GC

Trypanosomatidae: G3PDH

Plasmodium1/8/02

GENES NUCLEARES

CG10CdigoTOTAL%GC%GC1%GC2%GC12%GC3%GC3*TOTAL GC3*

P. falciparumPfa127228.380532.783030.896231.839621.462317.6923390

P.bergheiPbe127527.764729.176530.823530.000023.294120.6107393

P. knowlesiPkn127339.748638.679230.660434.669850.000054.1935465

P. vivaxPvi127245.283039.150931.603835.377465.094369.8718468

Chloroquine resistance transporter, para falciparum es putativo pero de muy alta homologa.

CDC2CdigoTOTAL%GC%GC1%GC2%GC12%GC3%GC3*TOTAL GC3*

P. falciparumPfa86433.333345.833332.291739.062521.875018.0952315

P.bergheiPbe86433.796345.486133.333339.409722.569425.0000300

P. knowlesiPkn86443.981550.000033.680641.840348.263956.2500336

P. vivaxPvi86444.791749.652832.638941.145852.083360.9091330

CDC2 protein kinase

CPCdigoTOTAL%GC%GC1%GC2%GC12%GC3%GC3*TOTAL GC3*

P. falciparumPfa170723.960234.446422.1