Investor Presentation

Pressure BioSciences, Inc.(OTCQB: PBIO)

Discovery Startswith Sample Preparation™

Investor PresentationMarch 2017

Richard T. SchumacherFounder, President, and CEO

Forward Looking Statements

This presentation may contain forward looking statements that reflect management’s current views and opinions as to the status of the Company’s products, technology and

other future events and operations. These statements are neither a promise nor guarantee, but involve risks and uncertainties that could cause actual results to differ

materially from those anticipated or indicated. Investors are cautioned that any forward looking statements should

be considered in light of such risks and uncertainties including, without limitation, those detailed in the

Company’s filings with theSecurities and Exchange Commission.

2

• 10 FT Staff; Near Boston, MA; Publicly Traded (OTCQB: PBIO)

• 15 Patents: Pressure Cycling Technology (“PCT”) Platform

• Develop, Mfg, & Sell PCT-based Instruments & Consumables

• Initial Focus in Sample Preparation for the Research Market

• ~ 275 PCT Systems Installed, 150+ Customers, 100+ Publications

• 30M Common Shares OS (75M CSE Fully Diluted)

• Revenue: FY2014 ($1.3M); FY2015 ($1.7M); Q1-Q3 2016 ($1.56M)

• 2016 Major Accomplishments: – Q3 2016: Record Product Revenue for Quarter and 9-month Period

– Closed $2M Line-of-Credit and $610K PIPE – both at favorable terms to shareholders – cleaned up BS (100% VRD Paid-off)

– Next Gen Barocycler 2320EXTREME Released; Multiple Units Sold for Cancer Moonshot Use

– Co-Marketing Agreement with SCIEX (WW analytical technologies leader) – a DHR Company

Company Overview (OTCQB: PBIO)

3

Management

• Mr. Richard T. Schumacher, President & CEOBoston Biomedica (CEO, Founder); Panacos Pharma (President, Co-founder);Trinity Biotech (Founding Group); CBR Labs (Gen’l Mgr) - Harvard Medical School

• Dr. Edmund Y. Ting, Senior VP of EngineeringAvure Technologies (CSO); Flow Int’l (CSO); Grumman Aerospace; MIT (Ph.D.)

• Dr. Alexander V. Lazarev, VP of R&D Proteome Systems; Genomic Solutions; ESA; University of Kazan (Ph.D.)

• Dr. Nathan P. Lawrence, VP of Sales & Marketing, Heads of ManufacturingBoston Biomedica; BD; Gene-Trak Systems; Yale University (Ph.D.)

Board• Jeffrey Peterson, MS Board Chairman• Kevin Pollack, Esq., MBA Audit Committee Chairman• Vito Mangiardi, MBA Compensation Committee Chairman• Mickey Urdea, Ph.D. SAB Chairman• Richard T. Schumacher CEO, Treasurer, Clerk

4

Experienced Senior Management & Board

Pressure Cycling Technology (PCT)Primary Sample Preparation for Biomolecular Analysis: Cell Lysis

5

Scientific research can be broken down to three key elements:sample preparation, analysis, & data reduction/interpretation

“Discovery Starts with Sample Preparation”

Sample Input Quality = Sample Result Quality

6

n QualitySample

Preparation

Quality Sample Results

• Analysis of DNA, RNA, Proteins, & Lipids (“biomolecules”) is Crucial to the Discovery of New Biomarkers – Quality of Results Depends on the Quality of Preparation

• Biological Sample Preparation is a Multi-Billion Market Comprised of an Estimated 500,000 Scientists in 80,000 Research Labs Worldwide

• Current Sample Preparation Methods are Mostly Mechanical and are Highly Inadequate: Bottleneck

• Proven Platform: ~ 275 PCT Systems Installed (~150 Sites)

• 100+ Publications Highlight Advantages of PCT Platform Over Other Methods

Value Proposition: PCT-based Biological Sample Preparation

7

Estimated Market

• Genomics (DNA/RNA) $7.1B in 2015 b

• Next Gen Sequencing (DNA) $2.7B in 2017c

• Mass Spectrometry (Proteins) $2.7B in 2011a,d

• Forensic Tools and Products (DNA) $17.7B in 2019 e

• Sample Prep for all OMICS: $8.4B in 2016 f

• 500,000 Scientists in 80,000 Biological Research Labs WW a

a EMMES Group b Decisive Bio-Insights c Markets & Markets

d TechNavio e Transparency Market Rsh f bcc Research

Biomarker/Forensics Market: Estimated Size

8

Selected Marquee Customers (150)

9

Barocycler™ NEP2320

PCT Sample Preparation System

Barocycler™ 2320 EXTREME

10

PULSE Tube

MicroTubes PCT-dependent Kits

Just Released ‼

PCT Platform: Enabling the Enablers

Eight Impact AreasWhere PBI’s Powerful and Enabling

PCT Platform is Currently Being Used…

1. Agriculture/Food Science 2. Environmental Epidemiology

3. Anti-Bioterror 4. Rational Drug Design

5. Biomarker Discovery 6. Fecal Lipidomic Profiling

7. Personalized Medicine 8. Improved Rape Kit Testing

11

Professor Wayne L. HubbellUCLA

• Distinguished Professor of Biochemistry• Member, National Academy of Sciences • Fellow, American Academy of Arts and Sciences

Molecular structure of a protein and the conformational changesthat control its function are key to better drug design and development

Conclusion

Response to Pressure May Have Profound Implications in Future Drug Design & Development

12

Rational Drug Design and Development

Professor Jennifer Van EykCedars-Sinai Medical Center

• Professor Of Medicine• Director, Basic Science Research,

the Barbara Streisand Women’s HC

• Over 1 billion archived formalin-fixed paraffin-embedded (FFPE) samples• Combination of heat and elevated pressure increased protein yield from

FFPE aorta samples, resulting in significantly more proteins identified

Conclusion

Results suggests that archival and often rare FFPE aortas and other tissues from patients with clinical histories that often span decades could potentially be invaluable if made available for PCT-enhanced

biomarker discovery and quantification 13

Biomarker Discovery

Non-invasive Fecal Lipidomic Profiling

Professor Bruce KristalHarvard Medical School

• Neuroscientist, Brigham and Women's Hospital, Boston• Associate Professor of Neurosurgery,• Molecular Biologist, Expert in Metabolomics

• Better analysis of fecal material will facilitate the understanding of gut (patho)physiology and its role in health and disease and will helpimprove care for individual patients, especially infants

• Pressure cycling differentially increased the yield of a number of lipids

ConclusionsNon-invasive fecal lipidomic profiling to define gut function

in health and disease could help physicians assessintestinal function, determine gut response to nutrition,

and recognize existing inflammation/pathology…especially valuable in infants and the elderly 14

Professor Bruce McCordFlorida Int’l University

• Professor of Analytical/Forensic Chemistry, • Over 75 peer reviewed papers and 11 book chapters

An estimated 400,000 untested rape kits in the USA(The Dark Side of Clearing America’s Rape Kit Back-log, Time Magazine 2013)

• Current studies focus on a novel technique to extract biological fluids fromcotton swabs with high recovery using the PCT platform

ConclusionShort extraction times, high yields, inexpensive reagents

and semi-automated platform make alkaline lysis-based PCTa promising candidate for extracting DNA from rape kits with the

potential additional benefit of reducing the current backlog 15

Improved Rape Kit Testing

Professor Ruedi Aebersold• Inst of Molec Sys Biology - ETH Zurich

• Professor of Systems Biology • Scientific Advisory Committee of numerous public and

private research organizations; member of severaleditorial boards in proteomics & genomics

Rapid mass spectrometric conversion of tissue biopsy samplesinto permanent quantitative digital proteome maps. Nature Medicine March 2015

Conclusion

Authors expect wide applications of PCT-SWATHin personalized medicine, and believe that PCT-SWATH may

ultimately achieve clinical adoption16

Personalized Medicine: Analysis of Tissue Biopsies

Weild & Co. HoldingsCEO, Co-Founder

• Former Vice-Chairman of NASDAQ

• “Father of the JOBS Act” (Forbes Mag)

• Former Head of Global Equity Transactions,Prudential Securities

• Former President, PrudentialSecurities.com

• Former Head, Capital Markets Group of Grant Thornton LLP

Weild & Co. has been Retained to Consult & AdvisePBI Management & Board of Directors

Mr. David Weild IV

17

• Q1: Close of Over-Subscribed $5M PIPE at $6.3M

• Q1: 100% (almost $3M) of all Variable Rate Debt Eliminated

• Q1: Co-Marketing Partner Announced (Jan): SCIEX! • Global leader in life sciences analytical technologies, incl. Mass Spec • Wholly-owned subsidiary of the Danaher Corporation (NYSE: DHR)• PBI and SCIEX will Co-Promote PCT-SWATH

• Q2: Next Generation Barocycler 2320EXTREME Released –Multiple Units in the NCI’s Cancer Moonshot Initiative (CMRI, etc.)

• Q3: Record Products Revenue for Q3 and YTD

• Q4: Close of $2M Line-of-Credit and $610K ($2.5M PIPE)

• Q4: Cancer Moonshot Initiative Signed into Law – PBI Reasserts Continuing Focus on this Opportunity

Key Achievements: 2016

18

Children’s Medical Research Institute (Australia)

19

SCIEX Announces Alliance with CMRI on Feb 3, 2016

CMRI Designated Official Collaborator – US Cancer Moonshot –by VP Joe Biden on July 16, 2016

• CMRI will test 70,000 tumor samples by PCT-SWATH

• CMRI has purchased 4 SCIEX MSs, 3 PBI Barocyclers & PCT Consumables

• First of what could be many centers WW for “industrialized proteomics”

Dr. Phil Robinson, Co-Head of CMRI, said: “sample size has In the past limited the use of mass spec-based proteomics

in clinical researchbut the use of PCT will now allow researchers to analyze

tissue samples as small as ones provided by needle biopsies”.

Initial Commercial Application: Mass Spectrometry (“First Killer App”)

20

The Following Information is for Discussion Purposes Only – Not Guidance

Biomarker Discovery (Enhanced Mass Spectrometry Sample Preparation)

We Believe:• there are approximately 16,000 MS labs worldwide • most MS protein analyses require extraction then digestion of proteins prior to processing• PCT offers significantly better protein extraction and digestion than current methods• In scientific research, quality counts even if it is more expensive

We Further Believe:

• an average client would require one Barocycler 2320EXTREME ($30,000) and use ~$12,000/year in consumables

• if PBI achieved 20% (minimum) market share over five years• 3,200 customers x $30,000/instrument (net) = $96M total, or $19.2M/yr • 3,200 customers x $12,000/yr in consumables (net) = $38.4M/yr (recurring revenue)

• Exclusive, 2-Year, WW, Co-Marketing Agreement with Global Leader SCIEX

• 100% of Floorless Debt Eliminated; $2M Line Closed; $610K PIPE Close

• 2017 M$S Expansion: 4+ Additions in Sales & Marketing

• Company Expects Continued Strong Revenue Growth

• Company Considering Up-list to NASDAQ in Early 2017

• Next Generation Barocycler 2320EXTREME Completed & Released (June 2016)

• Barocycler 2320EXT Units to be Used in “Cancer Moonshot” initiative

• Company Expects Collaborations with Key Opinion Leaders to Continue, Grow

• Company Expects Revenue Growth to be Driven by:• New Micro-Pestle Consumable• Just Released Next Generation Barocycler 2320EXTREME• New Focus on Barozyme HT48 & Barocycler HUB Instruments in 2017• SCIEX Co-Marketing Agreement• Additions to the Sales & Marketing Team in 2017

Short-term Growth Drivers

21