1

Name: ___________________________

CHEMISTRY 333 HOMEWORK # 2

DUE FRIDAY OCTOBER 23, 2009 (NO LATER THAN 5 PM!) SHOW ALL OF YOUR WORK AND HAND IN ALL GRAPHS

(35 points) 1. (20 points) The kinetics of an enzyme were analyzed in both the absence and presence of Inhibitor A and Inhibitor B. Given the following data, calculate or construct the following for A and B on separate graphs.

a) Plot the data as a Michaelis-Menten saturation curve. b) Estimate the Km and Vmax from these curves both in the presence and

absence of inhibitors. c) Plot the data in the Lineweaver-Burk format

*Make sure to label the both the inhibitor line and the no inhibitor line d) Mathematically determine the Km and Vmax. e) What types of inhibitors are A and B? How can you tell? f) On each graph draw a line that would indicate an increase in the

concentration of inhibitor. *Make sure to turn in all your plots, corresponding equations and calculations.

V (mmol/min) [S] (mM) No Inhibitor B (0.1mM)

.2 5.0 2.0

.4 7.5 3.0

.8 10.0 4.0 1.0 10.7 4.3 2.0 12.5 5.0 4.0 13.6 5.5

2. (15 points) Draw the following disaccharides in Haworth projections. Label the anomeric carbon with a * and circle any reducing ends.

a) galactose β(1,4) mannose b) glucose β,α(1,1) galactose

c) fructose α(2,5) ribose

V (mmol/min) [S] (mM) No Inhibitor A (5mM)

.2 5.0 3.0

.4 7.5 5.0

.8 10.0 7.5 1.0 10.7 8.3 2.0 12.5 10.7 4.0 13.6 12.5

V (mmoVmin

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V (mmoUmin)

lsl (mM) No Inhibitor A (5mM) 1/ ts l LIV (no I) Llv (+A)0.2 5 a

J 5 o.2 0.3333333330.4 7.5 5 2.5 0.133333333 0.20.8 l0 7.5 L.25 0.1 0.1333333331 t0.7 8.3 1 0.0934579440. 12048 L9282 12.5 10.7 0.5 0.08 0.0934579444 13.6 12.s 0.25 o.o735294L2 0.08

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V (mmoUmin)

lSl (mM) No Inhibitor B (0.1mM)

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V (mmoVmin)

tSl (mM) No Inhibitor B (0.1mM) 1/ rs l LIV (no I) Llv (+B)0.2 5 2 5 0.2 0.50.4 7.5 a

J 2.5 0.1333333330.3333333330.8 l0 4 t .25 0.1 0.25t r0.7 4.3 1 0.093457944 0.232558142 12.5 5 0.5 0.08 0.24 13.6 5.5 0.25 0.0735294L2 0.181818182

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