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Project leader: Daniela GoltsmanProject leader: Daniela Goltsman
Jose Villanueva, Victoria Partida,Jose Villanueva, Victoria Partida,
Katherine CantareroKatherine Cantarero
Microbial DiversityMicrobial Diversity
Table Of Contents. . .Table Of Contents. . .
• Introduction
• Inoculation
• Isolation
• FISH
• Results
• Conclusion
BackgroundBackground
• Why are microorganisms important?
• How are microorganisms studied today?– Aseptic technique – Dyes
• What was the goal of our experiment? – To study microbial diversity around our
environment
Sampling SitesSampling Sites
• Our samples:– Jose’s bathroom– UC Berkeley’s Strawberry Creek – Foothill Rec. Room Keyboard– Katherine’s Dorm
• Why did we choose these samples?
What is inoculation?What is inoculation?
• The introduction of microorganisms into any media.
• For us this process took about three days to complete.
• We incubated at 37 00 C
IsolationIsolation
• Taking one colony or “population” of one type of microorganism and making those populations reproduce and grow.
What is FISH?What is FISH?
• FISH: Fluorescent In Situ Hybridization.
• This is a powerful technique that can be used to evaluate and analyze the presence of organisms in their community, their phylogeny, morphology, and number by targeting the 16S rRNA.
FISH probesFISH probes
• What is a probe?– Short sequence of DNA that hybridizes
(binds) to a segment of the 16S rRNA. – Each probe carries a different color dye.
• Our dyes:– 4 different probes 3 different colors– Blue- Gama proteobacteria & Eukaryotic cells– Green- Universal bacteria– Red- Beta proteobacteria
Sample 1 LAPTOP. Green: bacteria, red: Sample 1 LAPTOP. Green: bacteria, red: beta-proteobacteriabeta-proteobacteria.
Sample 2: STRAWBERRY CREEK Green: universal Sample 2: STRAWBERRY CREEK Green: universal bacteria, red: beta-proteobacteria, soft blue: gama-bacteria, red: beta-proteobacteria, soft blue: gama-
proteobacteria, bright blue: eukaryotes.proteobacteria, bright blue: eukaryotes.
Sample 3:KATHERINE’S ROOM Red: Sample 3:KATHERINE’S ROOM Red: beta-proteobacteria, blue: eukaryotes.beta-proteobacteria, blue: eukaryotes.
Sample 4. Green: universal bacteria, Sample 4. Green: universal bacteria, blue: eukaryotesblue: eukaryotes
ConclusionConclusion
In conclusion, we learned that:
– Microorganisms are EVERYWHEREEVERYWHERE!– There are many kinds of bacteria that take on
many shapes and sizes.– Keyboards and bathrooms are the most
diverse–