Project-wise Reports of Sectoral Monitoring Committee

1. Project Title:lntegrated NextGen approaches in health, disease and environmental toxicity (INDEPTH)

2. Project Details:

Nodal lab CSIR-Indian Institute of Toxicology Research (IITR), Lucknow

Participating Labs CSIR-CDRI, CSIR-CIMAP, CSIR-CLRI, CSIR-CCMB, CSIR-IGIB, CSIR-IICB, CSIR-IHBT, CSIR-NBRI, CSIR-NCL, CSIR-NIO, CSIR-NEERI, CSIR-NEIST, CSIR-NIIST

Nodal Officer Dr DevendraParmar

Taskforce Dr AlokDhawan, Director, CSIR-IITR, Lucknow Chairman Total Man Days 31,885

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Approved Cost Rs. 5375.32 lakhs

Fund received Rs. 3549.9881akhs

Fund utilized Rs. 2991.7151akhs

3. Periodic Key Recommendations of Task Force/RC/Monitoring Committee

S.No Key Recommendations of Action taken on Key Proposed Revised Task Force/RC/Monitoring Recommendations Deliverabl Deliverable Committee es (approved

by Task Force)

i Awareness about the perchlorate Organised the awareness camps N/A N/A contamination in Kerala be made with local panchayat and to public community health centres for the

benefit of people living in the affected location

ii. To survey more number of Informed the committee on ths N/A N/A community kitchens and create initiatives to include more number awareness among the kitchen of community kitchens and workers on the health issues. suggested remedial measures to

the authorities iii Results of the epigenetic studies Results shared and NEERI N/A N/A

be shared among the scientist visited IITR to share his participatinQ labs. findinQs.

iv Studies dealing with proteomic Studies were restricted to identify N/A N/A approaches should be restricted possible protein based biomarkers on toxicity mechanisms or for different toxicity mechanisms environment induced diseases and environment induced

neurodegenerative diseases

v Stressed on networking among Discussions were held among the N/A N/A the partners working on participating labs and microbes etc bioremediation of oil studge, were shared between the labs. chromium removal and

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perchlorate decontamination

vi Emphasis be placed on patents Discussed among the participants N/A N/A and technologies and wor'king on future leads

4. Project Achievements:

S. No.

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Deliverables p(omised at start ,.;, ''.;'

New ::aria .~ sensitive' methods for quantitative evaluation of environmental contaminants (pyrethroids, organophosp-hate pesticides, phenols, polycyclic aromatic hydro-carbons, PAHs, and their biotransformed products) that are released in the environment. Identification of potential sources/inventories of perchlorate at various regions in Kerala and correlation with thyroid related effects. Identification of molecular pathways and cellular signaling mechanisms for characterization of toxicity of environmental chemicals (such as organophosphate pesticides, pyrethroids, metals and metal mixtures) and food contaminants. Delineation of mechanism of action of toxic substances on embryogenesis, tumorogen-esis, neurogenesis and management of heavy metal induced metabolic disorders.

Genetic variation responsible for differences in susceptibi lity to metals and pesticides (organophosphates) using Drosophila.

Identification of proteins modulated in response to environmental toxicants (pesticides, metals) for understanding toxicity mechanisms.

Isolation and identification of hyperaccumulator plants for metals and bacteria for the degradation of persistant chlorinated chemicals and

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~liverables ·Achi~ved ..

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Developed and validated novel automated methods for quantitative evaluation of environmental chem.­icals, contaminants in different environmental matrices

Identified potential sources of perchlorate in various districts of Kerala, correlated with thyroid related effects and suggested preventive measures Identified molecular mechanisms involved in the toxicity of environmental chemicals (pesticides, metals etc)and food contaminants.

Identified mechanism of action of toxic substances on embryogenesis, tumorogenesis, neurogen-esis and management of heavy metal induced toxicity.

Demonstrated differential modulation of genes homologous between Drosophila and Caenorhabditise/egans in response to methyl parathion and provided insights to the genetic basis of susceptibility of organisms to environ­mental agents Using proteomic approaches, identified a set of proteins that could be used a marker for predicting toxicities induced by pesticides as well as possible mark·ers for neurodegenerative diseases. Identified hyper-accumulator plants as well as terrestrial and marine microbes that -could be effectively used for the deqradation of persistant chlorinated

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If not achieved, reasons thereof

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hydrocarbon hydro-

5. Outcomesand outputs from the projects:

a. Outcomes 1. Lead(s) identified: Number and names with TRL

S. No. Leads TRL Status

i An application software using Indian prediction equation for spirometer in India 3 ii Extraction kit for analyzing multiclass residue analysis in environmental 1

matrices iii Development of biocompatible UV sensor 3 iv Hybrid epitopes of legume allergens for immunotherapy 3 v Peripheral biomarkers for predictinq environment induced toxicity/ diseases 1 vi Mass spectrometry based targeted quantification of advanced glycated albumin 1

and hemoglobin vii A bioprocess for decontaminating perchlorate (rocket fuel) contaminated water 5

and soil viii In situ bioremediation of Cr (Ill) removal in the contaminated soil- Laboratory 5

testing of integrated system ix Crude oil hydrocarbon bioremediation and eco-toxicity assessment 3 X Low cost "Green technology" for cleanup of toxic pollutant from tannery 4

/sewage sludge xi Low grain arsenic rice variety for safe human consumption 9

2. Technology/Product developed: Number and nameswith TRL level of Development Low grain arsenic rice variety developed by CSIR-NBRI for safe human consumption (CN1794-2-NBRI).

3. Technology/Product transferred: Number and nameswith name of licensee(s) Low grain arsenic rice variety developed by CSIR-NBRI for safe human consumption (CN1794-2-NBRI) has been successfully released by West Bengal Government for cultivation.

4. Facility created: Number and names with uniqueness & utility: N/A

5. Sponsored projects obtained (with details): Sponsored projects upto the tune of Rs. 3,44,26,0821akhs were received.

6. External Cash Flow (ECF) generated:Rs. 190 lakhs

7. Societal impact created:The project has led to the development of green chemistry based assays and lead to the improved understanding of the cellular and molecular mechanism of action of pesticides, metals.

The study on perchlorate decontamination in districts of Kerala and subsequent follow up actions by the panchayat and Kerala State health Dept. helped the recovery of a large number of people (-90) including childrens (-15) suffering from hypothyroidism in the affected areas.

A cross sectional study to assess the health status and urinary PAH metabolite measurements in indoor kitchens has led to the initiation of preventive measures for the safe health of indoor kitchen workers (419).

The transgenic rice variety developed was found to accumulate less arsenic and hopefully this product will help millions who consume arsenic contaminated food and water.

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Likewise, the use of algae and bacterial species for the removal of organic wastes along with metal uptake will benefit not only the leather industries but also in the treatment of lands contaminated with Cr(lll) back into economic use.

The bioprocess for removing the perchlorate or polyaromatic hydrocarbons (PAHs) will help in large scale bioremediation of oil sludge contaminated coasts, marine water and perchlorate contaminated water.

b. Outputs: 1. Periodic Reports Submitted

All interim reports submitted as and when requested

2. Publications i. Total number: 152 ii. CumulativeiF (as per2015figures): 541.667 iii. Average IF: 3.56 iv. Average IF/Scientist: 5/762

3. Patents (or other forms of IP generated such as Copyrights, Designs etc.) i. Filed: 8 ii. Granted: Nil iii. Licensed: Nil

4. Human Resource generated i. PhD fellows (give PhDs completed & pursuing separately)

23 h.Ds completed and 24 pursuing. ii. Project fellows: 15 project fellows iii. Skills imparted: Trained 128 M.Tech/ M. Pharma/ M.Sc students

5. External collaborations established

Lead/Technology/Prod Salient TRL of the IP Status of Status uct (listed above in Features of the Lead/ Lead/ DPR/Comm-

of

outcomes) Lead/Technology/ Product Techno· Techno!· ercial Technical logy/ ogy/Pro- Reportof Lead/ Product duct Technology/

Product An application software Software based in Indian 2 N/A N/A using Indian prediction norms can be installed in equation for spirometer existing spirometer. in India Kit for multiclass residue Green chemistry based. 1 N/A N/A analysis M icroextraction based

Development of Low cost 4 N/A N/A biocompatible UV sensor Ready to use.

No skill required for use.

Hybrid epitopes of Use of hybrid epitopes of 3 N/A N/A legume allergens for legumes for treatment of immunotherapy allerginicity, asthma

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Peripheral biomarkers for predicting pesticide induced genotoxicity, neurodegeneration

Mass spectrometry based targeted quantification of advanced glycated albumin and hemoglobin (CMV-Hb and CEV-Hb) A bioprocess for decontaminating perchlorate (rocket fuel) contaminated matrices (water, soil, etc.)

In situ bioremediation of chromium in the contaminated soil

Crude oil sludge bioremediation and eco­toxicity assessment

Elisa/ array based assay for 1 predicting genotoxicity

Prediction of diabetic 1 complications Prediction of prediabeticcondit- ions and diabetic nephropathy

Works using specific, TRL5 perchlorate reducing bacterial cocktail. Treat wide conc-entration range of perchlorate and high strength effluents (high salt, wide pH) containing perchlorate Remediation of soil is very fast (hours) Capital ahd operational costs are minimal Use of Cationic dispersant 5 immo-bi lized matrix for seq­uestering Cr(lll) from contaminated soil Safe treatment of oil sludge 3 waste. Acceptable process for safe environment

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6. Sectoral Monitoring Committee Recommendations on LeadfTechnology/Product (listed above): For each Lead/Technology/Product provide details separately:

Criteria SMC Recommendations Name of Leadrrechnology /Product • Bioremediation of perchlorate,

chromium, crude oil sludge, sewage water.

• Prediction equation software for indoor air pollution.

• Kits for monitoring multiclass residues, rapid and cost effective analytical technologies amenable for regulatory compliances.

• Low arsenic containing rice has been developed and released for cultivation

- in West Bengal. Is the Lead/ Technology/Product worth taking Yes up further?

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What is the further R&D efforts that need to be • Further developmental efforts should be put by CSIRICSIR laboratories? carried out to take the leads in

bioremediation, software and kits for monitoring to the next stage.

• Efforts should be made to popularize cultivation of low arsenic containing rice variety.

Global benchmarking of the All of the products developed are globally Lead/Technology/Product specifically wrt competitive and have commercialization specifications and cost potential. Is the Lead/Technology/Product worthy of Yes commissioning a DPR at this stage? What are the likely resources and time Financial support would be required for 3 duration required for taking forward the years identified Lead/ Technology/Product to the desired TRL? Potential stake holders who may be ISRO, Pollution Control Boards, appropriate to partner technically as well as Panchayats, Oil companies, Tanneries, financially Industries can partner technically as well

as financially. Suggestion for plausible road map towards • Interaction with industries, pollution further development of control boards for modalities, time lines lead/technology/product for achieving desired for bioremediation studies. TRL • Interaction with companies for

developing kits for chemical induced toxicity/ diseases.

GO/NO GO further development GO Other suggestions, if any, related to benefits/usage/ commercialization

7. Identified "lessons to be learnt", especially from shortcomings/failures:

8. Comments on financial progress on the project • fund availability and utilization of fund in the

project:

The financial progress is satisfactory and investment made is justified.

9. Sectoral Monitoring Committee Recommendations on Facility creation or other outcomes:

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10. Grading of Project Execution: Outstanding/ExcellenWery Good/Good/Satisfactory/Unsatisfactory

Very Good

Dr. S. Srikanth (Member)

Prof.,J.J). Singh (Member)

Prof. D. Narasimha Rao (Member)

/ dpi-~ Dr 1 Dharmalingam

(Member)

1,)/

Or. Ramesh Hariharan (Member)

~~ (Member)

~ Director, CSIR-IMTECH (TF Chairman • Member)

Prof. Seyed E. Hasnain (Member)

Dr. K. Sumathy (Member)

Director, CSIR-CCMB (TF Chairman • Member)

~~lki~~~-Director, CSIR-IICB (TF Chairman • Member)

Direc r, CSIR-IITR (TF Ch irman • Member)

~-~;!G, Padmanaban

(Chairman)

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