Prospect of endophytic fungi for

Jatropha curcas plantation on post

mining area

Utut Widyastuti, Hamim, Nampiah Sukarno, Sukma Gideon

Research Center for Bioresourches and Biotechnology –IPB; Unit for comercial services and industry (UJI) Department Biology-FMIPA IPB

Ecological damage on post tin mining activity

How are we save our soil to become more sustainable?

RevegetationJatropha curcas : can survive on marginal land;

Used for biofuel

Research Center for Bioresourches and Biotechnology –IPB; Unit for comercial services and industry (UJI) Department Biology-FMIPA IPB

Visi on 2010 (organic agriculture)

Biofretilizer:

Root mutualistic fungi

Biofertilizer from Outside country

Problem :Non-indigenous microbe

Indonesia

Rich on genetic diversity

Indigenous biofertilizer

Aspergillus sp

Control Aspergillus niger applicationwithout shaking

Aspergillus niger application with shaking

Enhanced germination of Jatropha curcasby Aspergillus niger

Treatment % Germinate

Dormant break down by heating

Aspergillus niger Filtrate 48,57

Control 30, 00

Non dormant break down

Aspergillus niger filtrate

31,43

Control 10,00

Enhanced germination of Jatropha curcasby Aspergillus niger

a. Control

A green house study

With lower concentrationof Phosfat

b. Aspergillus sp c. CMA

Kustiarini, 2003

How are Aspergillus sp effect for growing of Jatropha curcas on mining

activity soil ?

RevegetationJatropha curcas : can survive on marginal land;

Used for biofuel

Research Center for Bioresourches and Biotechnology –IPB; Unit for comercial services and industry (UJI) Department Biology-FMIPA IPB

Tin mining activity soils in P. Bangka-Belitung

Low pH < 4Heavy metal

Increased of growth jatropha curcas on tin mining activity soil with endophytic fungi

Control

5 month in tin mining area

5 month

1 month

Aspergillus niger treatment (250 g/hole)

Effect of endophytic fungi doses to growth Jatropha curcas on tin mining activity soil

25

26

27

28

29

30

31

32

33

34

35

1 2

Series1

Doses

500 g/hole 250 g/hole

Heigh

t (cm)

25

35

0

0.5

1

1.5

2

2.5

3

3.5

4

1 2

Series1

500 g/hole 250 g/hole

Num

ber of b

ranching

Doses

Performance of Jatropha curcas on tin mining area with endophytic fungi

10 month

Enhanced of growth Jatropha curcas in post tin mining area with combination of endophytic fungi, top soil and compost

25

26

27

28

29

30

31

32

33

34

35

1 2

Series1

0

0.5

1

1.5

2

2.5

3

3.5

4

4.5

5

1 2

Series1

Heigh

t (cm)

Num

ber of b

ranching

Aspergillus sp Aspergillus spCombination Combination

Enhanced of growth Jatropha curcaswith combination: endophytic fungi, top soil, compost

10 month

Conclusion

Treatment with Aspergillus niger as a biofertilizer provided nutriens to support plant growth

Endophytic fungi was needed for establishment ofJatropha curcas growth on post tin mining area.

Research Center for Bioresourches and Biotechnology –IPB; Unit for comercial services and industry (UJI) Department Biology-FMIPA IPB

Acknowledgement