Pseudomonas IdentificationPseudomonas Identification

Done ByDone By : :

Omniya TayemOmniya Tayem

Dr: Adbelraouf ElmanamaDr: Adbelraouf Elmanama

Mohamad LaqanMohamad Laqan

Structure & PhysiologyStructure & Physiology

* *Gram _ Negative rodsGram _ Negative rods

Gram negative rodGram negative rod

Motile with polar flagellaMotile with polar flagella **

Motility positiveMotility positive

Obligate aerobeObligate aerobe

Oxidase _ positiveOxidase _ positive* *

Do not ferment Do not ferment Carbohydrates Carbohydrates

Resistant to multiple drugsResistant to multiple drugs

Majority of human infectionsMajority of human infections

_ _ P. aeruginosaP. aeruginosa

Common in the environmentCommon in the environment

. .WaterWater

. .AirAir

..SoilSoil

Very simple nutritional requirementsVery simple nutritional requirements

Many organic compound used asMany organic compound used as

C & N sources , but onlyC & N sources , but only

afew carbohydrates byafew carbohydrates by

oxidative metabolismoxidative metabolism

Some strains appear mucoid due toSome strains appear mucoid due to

Polysaccharide CapsulePolysaccharide Capsule

Some produce diffusible pigmentsSome produce diffusible pigments

PyocyaninPyocyanin _ _

nonfluorescent bluish pigmentnonfluorescent bluish pigment

PyoverdinPyoverdin _ _

Fluorescent greenish pigmentFluorescent greenish pigment

Pyorubin & PyomelaninPyorubin & Pyomelanin

Fluorescent PigmentsFluorescent Pigments

Pseudomonas . aeruginosaPseudomonas . aeruginosa

Pseudomonas aeruginosa colonies Pseudomonas aeruginosa colonies on Agaron Agar

Pseudomonas . aeruginosaPseudomonas . aeruginosa

Identification of P. AeruginosaIdentification of P. Aeruginosa

Usually based onUsually based on

colonial morphologycolonial morphology , ,

B- hemolysisB- hemolysis , ,

Oxidase positivityOxidase positivity , ,

The presence of characteristic pigmentsThe presence of characteristic pigments

& &Sweet odorSweet odor

& &Growth at 42cGrowth at 42c

Identification Of PseudomonasIdentification Of Pseudomonas

PigmentsPigments **Pyocyanin (blue _ green )Pyocyanin (blue _ green )

Fluorescein (green _yellow_fluorescent )Fluorescein (green _yellow_fluorescent )

Pyorubin (red _ brown )Pyorubin (red _ brown )

Culures have fruity smellCulures have fruity smell **

Biochemical reactionsBiochemical reactions* *

..Gelatine hydrolysisGelatine hydrolysis

..Starch hydrolysisStarch hydrolysis

..Carbohydrates useCarbohydrates use

Trehalose (O_F medium)Trehalose (O_F medium)

..Flourescent PigmentFlourescent Pigment

P. aeruginosaP. aeruginosa

Gelatin hydrolysis Gelatin hydrolysis +/ - +/ - ( ( variable result variable result ))

Starch hydrolysis Starch hydrolysis + / - + / - ( ( variable result variable result ))

Acid Aerobically from D_ TrehaloseAcid Aerobically from D_ Trehalose

) ) negativenegative ( (

Fluorescent pigment ( Fluorescent pigment ( positive positive ))

Non Fluorescent pigment ( Non Fluorescent pigment ( positive positive ))

P .fluorescensP .fluorescens

Gelatin hydrolysis ( Gelatin hydrolysis ( positive positive ))

Starch hydrolysis ( Starch hydrolysis ( negative negative ))

Acid aerobically from D _ Trehalose Acid aerobically from D _ Trehalose

( (positivepositive))

Fluorescent pigment ( Fluorescent pigment ( negative negative ))

Non fluorescent pigment (Non fluorescent pigment (positive positive ))

P. SaccharophilaP. Saccharophila

Gelatin hydrolysis (Gelatin hydrolysis (PP))

Starch hydrolysis (Starch hydrolysis (PP))

Acid aerobically from D_ Trehalose (Acid aerobically from D_ Trehalose (P P ))

Fluorescent pigment ( Fluorescent pigment ( N N ))

Non fluorescent pigment ( Non fluorescent pigment ( N N ))

P. PutidaP. Putida

Gelatine hydrolysis ( Gelatine hydrolysis ( negative negative ))

Starch hydrolysis (Starch hydrolysis (negative negative ))

Acid aerobically from D_ Trehalose (Acid aerobically from D_ Trehalose (N N ))

Fluorescent pigment ( Fluorescent pigment ( P P ))

Non fluorescent pigment ( Non fluorescent pigment ( P P ))

The EndThe End