Pump 11 Elite & Pump 11 Pico Plus Elite cfile.yizimg.com/528182/20171129-172413520.pdfHarvard Apparatus Pump 11 Elite & Pump 11 Pico Plus Elite Series Publication 5420-002 Rev B w

Harvard Apparatus Pump 11 Elite& Pump 11 Pico Plus Elite SeriesPublication 5420-002 Rev B

www.harvardapparatus.comPump 11 Elite &

Pump 11 Pico Plus Elite

USER’S MANUAL

phone 800.272.2775email [email protected]

2Table of Contents

SUBJECT PAGE NO.

Table of Contents

General Information ................................................................................................6

Safety Information..................................................................................................6

Warranty and Repair Information ..........................................................................7

Manual Description ....................................................................................7

Warranty......................................................................................................7

Repair Facilities and Parts ..........................................................................7

Serial Numbers............................................................................................7

Calibrations..................................................................................................7

Specifications ........................................................................................................8

Product Overview....................................................................................................9

Pump Models ......................................................................................................10

Pump 11 Elite Series Touch Screen Button Reference........................................11

Selecting an Item from a Menu List ....................................................................12

Messages ............................................................................................................12

Entering Values ....................................................................................................12

Operating Instructions..........................................................................................13

Power Connections ..............................................................................................13

Syringe Loading....................................................................................................14

Configuring the Pump 11 Elite Series..................................................................15

Method Selection ................................................................................................15

Syringe Selection..................................................................................................16

Flow Rate Selection ............................................................................................17

Target Volume/Time Selection ..............................................................................18

Previewing the Quick Start ..................................................................................19

Configuring the Pump’s General Parameters ......................................................19

Setting the Audible Alarms ..................................................................................20

For Near End of Run Selection ..................................................................21

For a Custom ............................................................................................21

Locking the Touch Screen ........................................................................22

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phone 508.893.8999 fax 508.429.5732 e-mail [email protected] www.harvardapparatus.com Pump 11 Elite Series Syringe Pump User’s Manual • Publication 5420-002 Rev B

3Table of Contents

Setting the Password ................................................................................22

Turning the Touch Screen Lock on ............................................................22

Turning the Touch Screen Lock off............................................................22

Setting the Force Limits ............................................................................23

Setting Display Backlight ..........................................................................24

Setting the Pump Address ........................................................................25

Setting the Baud Rate ..............................................................................25

Power-Up Running ....................................................................................26

Setting and Viewing Pump Information ....................................................26

Tilt ..............................................................................................................27

Operating the Pump 11 Elite Series ....................................................................28

Running the Pump................................................................................................28

Custom Method Workflow ..................................................................................29

Configuring Custom Methods ..............................................................................31

Configuring a New Custom Method ....................................................................31

Configuring Custom Methods ..............................................................................32

Setting a Delay ....................................................................................................33

Creating a New Custom Method ........................................................................34

To Create a New User-Defined Method ....................................................34

Modifying an Existing/User-Defined Method ......................................................36

Changing the Order of Steps in a Method ................................................36

Deleting a Step from a Method ................................................................36

Defining Method Step Parameters ......................................................................36

Pump Operation Profiles ......................................................................................37

Constant Rate Profile ................................................................................38

Ramp Profile..............................................................................................39

Gradient Profile..........................................................................................40

Autofill Cycling Profile ..............................................................................42

Incorporating Advanced Options Into a Method ..................................................43

Delay ........................................................................................................44

Repeat Step ..............................................................................................44

Stop ..........................................................................................................44

SUBJECT PAGE NO.

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4Table of Contents

I/O Event Triggers ................................................................................................45

I/O Trigger (OUT) ......................................................................................45

I/O Event (IN) ............................................................................................46

Managing Pump 11 Elite Series Methods............................................................47

Saving a Copy of a Method ......................................................................47

Renaming a Method..................................................................................47

Appending a Method ................................................................................47

Deleting a Method ....................................................................................47

Method Import and Export ..................................................................................48

Exporting a Method to an External Computer ..........................................49

Importing a Method from an External Computer ......................................49

External Pump Control ........................................................................................50

Setting up HyperTerminal ..........................................................................50

Pump Chain Commands ......................................................................................51

Using the Pump 11 Elite Series Pump Chain Commands ........................51

Error Messages ........................................................................................52

Command Errors ......................................................................................52

Argument Errors........................................................................................52

System Commands ..................................................................................52

Run Commands ........................................................................................54

Rate Commands........................................................................................54

Volume Commands ..................................................................................55

Time Commands ......................................................................................56

Digital I/O Commands ..............................................................................57

Internal Commands ..................................................................................57

Serial Command Notes ............................................................................57

SUBJECT PAGE NO.

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5Table of Contents

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Appendices ............................................................................................................58

Appendix A: Syringe Volume/Diameter Reference Table ....................................58

Appendix B: Min/Max Flow Rates........................................................................59

Appendix C: External Connections ......................................................................60

User I/O Connector Specifications ............................................................60

Utilizing USB Interface ..............................................................................61

RS-485 ......................................................................................................63

Legacy RJ-11 Option ................................................................................64

Appendix D: Maintenance ....................................................................................65

Maintenance..............................................................................................65

Upgrading Pump 11 Elite Series Software ................................................66

Troubleshooting ........................................................................................67

Appendix E: Ordering Information........................................................................68

SUBJECT PAGE NO.

6Table of Contents

General Information

SAFETY INFORMATION

Please read the following safety precautions to ensure proper use of your syringe pump. If the equipment is used in amanner not specified, the protection provided by the equipment may be impaired.

To Prevent Hazard or Injury:USE PROPER POWER SUPPLYThe pump is supplied with an approved power supply and line cord. To maintain the safety integrity of the device, useonly the following power supplies:

Cui Inc. Globtek Inc.Model: 3A – 242DB24 Model: GT–41062–T3Output: 24V – 1.0A Output: 24V – 0.75AInput: 100–240V~50–60 Hz 0.8A Input: 100–240V~50–60 Hz 0.6A

USE PROPER LINE CORDUse only the specified line cord for this product and make sure line cord is certified for country of use. The operatingvoltage range for the Pump 11 Elite Series is 12-30 VDC. The universal power supply operating voltage range is100-240 VAC, 50/60 Hz.

GROUND THE PRODUCTThis product is grounded through the grounding conductor of the power cord. To avoid electric shock, the groundingconductor must be connected to earth ground. Before making any connections to the input or output terminals of theproduct, ensure that the product is properly grounded.

MAKE PROPER CONNECTIONSMake sure all connections are made properly and securely. Any signal wire connections to the unit must be no longer than 3meters.

OBSERVE ALL TERMINAL RATINGSReview the operating manual to learn the ratings on all connections.

AVOID EXPOSED CIRCUITRYDo not touch any electronic circuitry inside of the product.

AVOID PINCH HAZARDA pinch hazard may exist between the pusher block and end blocks. Avoid placing fingers between these points whilethe pump is running.

DO NOT OPERATE WITH SUSPECTED FAILURESIf damage is suspected on or to the product do not operate the product. Contact qualified service personnel to performinspection.

ORIENT THE EQUIPMENT PROPERLYDo not orient the equipment so that it is difficult to operate the disconnection device.

PLACE PRODUCT IN PROPER ENVIRONMENTReview the operating manual for guidelines for proper operating environments.

OBSERVE ALL WARNING LABELS ON PRODUCTRead all labels on product to ensure proper usage.

ProtectiveGround Terminal

CAUTIONRefer to Manual!

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7General Information


WARRANTY AND REPAIR INFORMATION

Manual DescriptionThis manual is designed to provide all operational and Method information required to operate and maintain thePump 11 Elite Syringe Pumps. The functions and features are described in the Technical Specifications section.

WarrantyHarvard Apparatus warranties this instrument for a period of two years from date of purchase. At its option,Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or materials.This warranty does not extend to damage resulting from misuse, neglect or abuse, normal wear and tear, oraccident. This warranty extends only to the original consumer purchaser.

IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIALDAMAGES. Some states do not allow the exclusion or limitation of incidental or consequential damages so theabove limitation or exclusion may not apply to you. THERE ARE NO IMPLIED WARRANTIES OFMERCHANTABILITY, OR FITNESS FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states do notallow this limitation on an implied warranty, so the above limitation may not apply to you.

If a defect arises within the warranty period, promptly contact Harvard Apparatus, 84 October Hill Road,Holliston, Massachusetts 01746 using out toll free number 1-800-272-2775 or outside the US call 1–508-893-8999. Email address is [email protected]. Goods will not be accepted for return unless an RMA(returned materials authorization) number has been issued by our customer service department. The customer isresponsible for shipping charges for non-warranty repairs. Please allow a reasonable period of time for completionof repairs or replacement. If the unit is replaced, the replacement unit is covered only for the remainder of theoriginal warranty period dating from the purchase of the original device.

This warranty gives you specific rights, and you may also have other rights which vary from state to state.

Repair Facilities and PartsHarvard Apparatus stocks replacement and repair parts. When ordering, please describe parts as completely aspossible, preferably using a part number obtained from our Technical Support department. If practical, enclose asample part or sketch. We offer a complete reconditioning service.

Serial NumbersAll inquiries concerning our product should refer to the serial number of the unit, located on the rear panel.

CalibrationsAll electrical apparatus are calibrated at rated voltage and frequency. While the flow and volume will stay calibrated,the peak pressure may vary. Harvard Apparatus recommends an annual calibration of the pump.

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! CAUTION: FOR RESEARCH USE ONLY. NOT FOR CLINICAL USE ONPATIENTS.

!CAUTION: REFER TO SAFETY INFORMATION AND SETTING UP THEHARVARD APPARATUS PUMP 11 ELITE SERIES BEFORE PLUGGING INTHE PUMP.

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General Information

Specifications Pump 11 Elite Pico Plus EliteAccuracy ± 0.5% ± 0.35%

Reproducibility ± 0.05% ± 0.05%

Syringes (Min./Max.) 0.5 µl / 60 ml (10 ml dual) 0.5 µl / 10 ml

Flow Rate:

Minimum (0.5 µl syringe) 1.28 pl/min 0.54 pl/min

Maximum (10 ml syringe) 25.99 ml/min 11.70 ml/min

Maximum (60 ml syringe) 88.28 ml/min N/A

Display 4.3" WQVGA TFT Color Display with Touch Screen 4.3" WQVGA TFT Color Display with Touch Screen

Non-Volatile Memory Stores all settings Stores all settings

Connectors:

RS-485 IEEE-1394, 6 pos IEEE-1394, 6 pos

USB Type B Type B

I/O & TTL 15 pin D-Sub Connector 15 pin D-Sub Connector

Footswitch mini phono jack mini phono jack

Linear Force (Max) 16 kg (35 lbs) @ 100% Force Selection 16 kg (35 lbs) @ 100% Force Selection

Drive Motor 0.9° Stepper Motor 0.9° Stepper Motor

Motor Drive Control Microprocessor with 1/16 microstepping Microprocessor with 1/16 microstepping

Number of Microstepsper one rev. of Lead Screw

15,360 20,480

Step Resolution 0.069 µm/µstep 0.031 µm/µstep

Step Rate:

Minimum 27.5 sec/µstep 27.5 sec/µstep

Maximum 26 µsec/µstep 26 µsec/µstep

Pusher Travel Rate:

Minimum 0.15 µm/min 0.02 µm/min

Maximum 159.00 mm/min 71.55 mm/min

Input Power 12-30 VDC 12-30 VDC

Input Power Connection 2.5mm ID x 5.5mm OD male plug 2.5mm ID x 5.5mm OD male plug

Power Supply 100-240 VAC, 50/60 Hz, 8 Watts Universal PowerSupply, Use Only a Harvard Apparatus ApprovedPower Supply and Line Cord

100-240 VAC, 50/60 Hz, 8 Watts Universal PowerSupply, Use Only a Harvard Apparatus ApprovedPower Supply and Line Cord

Dimensions 22.6 x 17.78 x 15 cm (9 x 7 x 6 in) 22.6 x 17.78 x 15 cm (9 x 7 x 6 in)

Weight 2.1 kg (4.6 lbs) 2.1 kg (4.6 lbs)

Atmospheric Specifications:

Operating Temperature 4°C to 40°C (40°F to 104°F) 4°C to 40°C (40°F to 104°F)

Storage Temperature -10°C to 70°C (14°F to 158°F) -10°C to 70°C (14°F to 158°F)

Humidity 20% to 80% RH, non condensing 20% to 80% RH, non condensing

Method of Operation Continuous Continuous

Classification Class I Class I

Pollution Degree 1 1

Installation Category II II

Supplier Name Harvard Apparatus Harvard Apparatus

Supplier Address 84 October Hill Rd., Holliston, MA 01746 84 October Hill Rd., Holliston, MA 01746

Supplier Phone Number (508) 893-8999 (508) 893-8999

Regulatory Certifications CE, ETL (UL, CSA), WEEE, EU RoHS & CB Scheme CE, ETL (UL, CSA), WEEE, EU RoHS & CB Scheme

Safety Declarations ANSI/UL 61010-1:2004 ED. 2 Rev. 2005 ; CAN/CSAC22.2 No. 61010-1:2004 Ed 2 ; IEC 61010-1:2001Ed 2 Corrigendum 1:2002, Corrigendum 2:2003 ;CENELEC EN 61010-1:2001 ; CB Scheme

ANSI/UL 61010-1:2004 ED. 2 Rev. 2005 ; CAN/CSAC22.2 No. 61010-1:2004 Ed 2 ; IEC 61010-1:2001Ed 2 Corrigendum 1:2002, Corrigendum 2:2003 ;CENELEC EN 61010-1:2001 ; CB Scheme

EMC Declaration CENELEC EN 61326-1:2006 CENELEC EN 61326-1:2006

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Product Overview


Product Overview

The Harvard Apparatus Pump 11 Elite Series is thenext generation of syringe pumps. The series offersunparalleled ease of use through the high resolutioncolor touch screen user interface. The full touch screeninterface enables the user to quickly createconfigurations and recall them for easy use. The TFTcolor display presents all the pump operatingparameters on an easy to view run screen. The touchscreen “lock” feature prevents accidental configurationchanges ensuring the Methods integrity is retained.

A green led light on the front panel makes it easy to seeif the pump is running. Once the pump starts to run, thedisplay shows the pumps is running and the LED turnson. This will make it easy to see the pump’s status.

The pumps are versatile and can be interconnectedthrough the RS-485 interface. Pump 11 Elite Seriesmodels can be mixed and matched in the daisy chainoffering maximum flexibility. Up to 99 pumps can belinked together through the RS-485 interface. Thisinterface is easy to use provided each pump is assignedits own unique pump address.

For limited laboratory space the Pump 11 Elite Seriescan be placed on its side to reduce the footprint by55%. The display can also be tilted to allow the user tooperate the pump vertically.

Notifications and error messages are displayed for theuser to acknowledge, eliminating any guesswork aboutproblems.

Flow performance is optimized with a small step anglestepping motor that drives a precision lead screw andpusher block. The syringe mechanism is easy to useand securely holds the syringes for smooth flowperformance.

Advanced microstepping techniques are employed tofurther reduce the step angle to eliminate flowpulsation. The accuracy is 0.5% (0.35% for Pico PlusElite). A wide dynamic flow range from 1.28 pl/min to88.28 ml/min (0.54 pl/min to 11.70 ml/min for the PicoPlus Elite) can be programmed into the pump. Flowrates are selectable with user selected engineeringunits from ml, µl, nl, pl, and hours, minutes andseconds.

Up to 16 kg (35 lbs) of linear force is available. This forceis user adjustable to ensure the right force is applied forthe various syringe sizes.

The spill dam is designed to prevent fluids fromingressing into the sealed display or electricalconnections.

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Product Overview

Easy external connections to a computer or othercontrol devices are made through USB interface. SimpleASCII commands make communication with the pumpeasy. For direct control of the pump, the user can usethe I/O interface (15pin Dsub). One trigger output and atrigger input in for external events such as a processparameter are available. The footswitch input will allowthe control of the pump through an external device. Theunit also has an output for run indication allowingconnection to a remote LED.

The Basic and Programmable 11 Elite Pumps areavailable with single and dual syringe options. Completeflexibility is offered with the ability to use ANYmanufacturers syringes ranging from 0.5 µl to 60 mlsingle syringe and 5 µl to 10 ml dual syringe.

The programmable models offer maximum flexibilityand capability for configuring and running differentMethods. Methods can be configured and stored in theunit, and recalled quickly with the touch of the screen.The Methods are identified with a 15 characteralphanumeric name for easy identification and can bestored on the computer and downloaded at futuredates. Extensive programming options allow the user toduplicate configurations, append one configuration toanother, rename Methods, delete Methods, exportMethods to a PC or export a Method to another pump.This feature can ensure consistency and accuracy ofmultiple pumps.

Preprogrammed profiles are selectable from ConstantRate, Ramp, Autofill and Gradient. These standardprofiles make configuration easy to set up. If morecomplexity is needed the user can select from advancedMethod programming options including:

• Time delays

• Repeating steps

• Stopping the pump

• Triggering the pumps TTL output

• Accepting an event input in such as a usertouch or motor stall

• Events can trigger the pump to withdraw orinfuse

The pump Information/settings screen shows thepump’s software version, maintenance interval andpump address. The pump’s alarm configurabilityincludes alarms for near end of run (user selectable),completion of run, power up, keypad clicks and stalldetection.

Pump ModelsInfusion Only –(single and dual syringe models): These two modelssupport infuse only Quick Start Methods withuser-defined flow rates and selectable target volume ortarget time values to control the total infusion time.

Infusion/Withdrawal Programmable –(single and dual syringe models): These two modelssupport infuse only, withdraw only, infuse/withdraw andwithdraw/infuse Quick Start Methods with user-definedflow rates and selectable target volume or target timevalues to control the total infusion time. These modelsalso support two user-defined Methods up to 50 stepseach. Two Methods can be stored.

Pico Plus Infusion/Withdrawal Programmable –(dual syringe only): Supports all four Quick StartMethods and two user-defined Methods up to 50steps each.


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Product Overview

PUMP 11 ELITE SERIES TOUCH SCREEN BUTTON REFERENCE

The following buttons are part of the Pump 11 Elite Series software:

Settings: Access the settings menu usedto adjust the following parameters:Audible Alarms, Pusher Block Force,Backlighting, Baud Rate, etc.

Fast Forward: Advances the Pusher Blockin the forward (infuse) direction.

Fast Reverse: Advances the Pusher Blockin the reverse (withdraw) direction.

Accept: Used to accept theparameters/settings on a screen andadvance to next screen in the menu.

Cancel: Used to cancel anychanges/entries on a screen and return tothe previous screen in the menu.

Back: Used to go back one screen.

Set Delay Parameters: Used to set thetime values for delayed operation. Thispermits the pump to start automaticallyafter a preset time.

Set Delay Parameters: Indicates theDelay Timer has been set. The pump willstart automatically after the preset delayexpires.

Run Preview: Access the MethodPreview screen that shows a summary ofsteps and operating parameters.

Run/Resume: Used to Run or Resume aMethod.

Re-Run: Clears counters/timers and runsthe selected Method from the beginning.

Stop: Used to stop running a Method.

Clear: Clears the counters/timers.

Minimum Value Allowed: Used to selectminimum value allowed.

Maximum Value Allowed: Used to selectmaximum value allowed.

Page Down: Used to page down in adisplay list.

Page Up: Used to page up in a displaylist.

Scroll Up: Used to scroll up in a displaylist.

Scroll Down: Used to scroll down in adisplay list.

Insert Step: Used to insert a new Step ina Method while editing/creating theMethod. The new Step is inserted afterthe Step that is currently selected.

Delete Step: Used to delete the selectedStep in a Method while editing/creatingthe Method. To confirm deletion, youmust select the Delete Step button asecond time.

Move Step Up: Moves the currentlyselected Step in a Method up oneposition in the Method Order (e.g. Step 3becomes Step 2).

Move Step Down: Moves the currentlyselected Step in a Method down oneposition in the Method Order (e.g. Step 2becomes Step 3).

Copy Step: Creates a duplicate (copy) ofthe currently selected Step in a Methodand inserts it as the last Step in theMethod being created/edited.

Touch Screen Unlocked: Indicates thatthe Pump is unlocked. Pressing thebutton twice locks the touch screen.

Touch Screen Locked: Indicates that thePump set-up screens are locked fromediting. Press the button and enterpassword to unlock the touch screen.

File Options: Access the File Optionsmenu that is used to manage the list ofMethods and to export Methodinformation to an externally linked pumpor to a connected computer.


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Product Overview

Selecting An Item From a Menu ListSelecting items is easy with the Pump 11 Elite Series.

Touch the item to select/highlight the item,then touch again to accept it or press

Canceling a SelectionCanceling selections is easy.

Press

MessagesThere are different types of messages. Error messagesare shown in yellow.

• Critical• Run--mode• Method• Settings

Notices, alarms, confirming, and verified messages areshow in green.

• Alarms• Confirm• Verified

Entering ValuesEntering new values is easy.

Use the keypad to enter the number

Backspace key

Decimal point

Cancel and go back tothe previous screen.

Accept the value and go backto the previous screen.


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Product Overview

OPERATING INSTRUCTIONS

POWER CONNECTIONS

1. Plug the power cord in. The Pump 11 Elite Series has a universal power input connector on the rear of theunit. The operating range for the Pump 11 Elite Series series is 12-30 Vdc.

2. Turn on the main power switch.

!CAUTION: DO NOT CONNECT TOFIREWIRE PORTS ON A COMPUTER.DAMAGE MAY OCCUR TO THEPUMP OR COMPUTER.

*I/O Connector

Foot Switch

Main Switch

UniversalPower Input

*RJ-11 Option

*Power Source -When powering apump in a chainthrough an RS-485cable, toggleswitch to RS-485.Only one pump canbe powered frommaster throughRS-485*RS-485 Connection

(Pump to Pump)

* Programmable pumps only

USB Serial Input(Type B)

Pusher Block

Guide Rod

Half NutButton

Lead Screw

User InterfaceScreen withProtective

Antiglare andEMI Shield

Retaining Brackets

Syringe Barrel Clamp

Syringe Holder

Bracket Clamp Knobs

Run LED


Best performance guaranteed when using24V power supply.

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Product Overview

5. Screw down the syringe clamp knob (6) untilthe syringe barrel clamp (10) is tight againstthe syringe barrel. Do not overtighten,especially when using glass syringes.

6. Secure the syringe flanges by pushing theretaining brackets (4,5) tightly against thesyringe flanges using the bracket clampingknobs (7,8). The syringe plunger should besecured by the pusher block retaining bracketand the flange of the syringe should besecured by the syringe holder block retainingbracket.

7. Tighten the mechanical stop collars (9) usingallen wrench (12) to prevent excess travel ofthe pusher block.

SYRINGE LOADING

The Pump 11 Elite Series can accommodate up to twosyringes. Depending on the mechanism, any type ofsyringe, glass, plastic or stainless steel can be used.The Pump 11 Elite can accommodate from 0.5 µl to 60ml single syringe and 0.5 µl to 10 ml dual syringe. Thesyringe barrel clamp should be flipped for syringessmaller than 30 ml. For syringes larger than 30 ml thesyringe clamp should be used as shown.

1. Press the release button (1) and move thepusher block (2) to the approximate length ofthe syringe.

2. Unscrew the bracket clamping knobs (7,8) toloosen the retaining brackets (4,5) on thepusher block (2) and the Syringe holder block(3).

3. Unscrew the syringe barrel clamp byunscrewing the syringe barrel clamp knob (6).

4. Place the syringe on the syringe holder block(3). Ensure the barrel flange is with in thesyringe holder’s block’s retaining bracket (5)and the plunger flange is in the pusher block’sretaining bracket.

*The Pico Plus Elite can accomodate from 0.5 µlto 10 ml.

!CAUTION: BE SURE MECHANICALSTOP COLLARS ARE POSITIONEDPROPERLY TO PREVENT THEPLUNGER FROM BOTTOMING OUTON GLASS SYRINGES.

> 30 cc syringes

< 30 cc syringes

2 4 5 3

6

7

1

9

10

8

11


The syringe barrel clamp (10) may need to beflipped depending on your syringe size. To doso, completely unscrew and remove thesyringe barrel clamp knob from the syringeblock. Then remove lock nut (11). Flip thebracket and reassemble.

15Configuring the Pump 11 Elite Series

A Method is easy to set up.

The Pump 11 Elite Series is easily configured.When the unit is powered up, the Quick StartScreen will be displayed.

Configuring the Pump 11 Elite Series

SelectFlow Rates

SelectTarget Volume

Fast Reverse Fast Forward

StartsPump &Jumps toRun Screen

PreviewMethodJumps toRun Screen

GeneralPumpParameterSettings

Method SelectionIn the Quick Start Screen select Method(available Methods depend on the pump model)

• Infuse Only• Withdraw Only• Infuse/Withdraw• Withdraw/Infuse• Custom Method

The Method Selection Screen will be displayed. Theavailable Methods will be displayed based on themodel of syringe pump.

Press the Method to select

Press and hold to activate

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SelectMethod

SelectSyringe

ManufacturerSize


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Syringe Selection

1. In the Quick Start Screen select thesyringe button.

2. Select the manufacturer type. Then theavailable syringe sizes will be displayed. Inthis example we have selected a BectonDickinson Syringe.

3. For a Custom Syringe use thisselection if syringe manufacturer isnot listed.

Custom Syringe Definition Display

!CAUTION: BE SURE TO LOWERTHE FORCE VALUE WHEN USINGGLASS SYRINGES.

Press

Press

Press toenter acustomsyringe

Press to enter the insidediameter of the syringe

Press to enter thetotal syringe volume

Press to changethe units (µl, ml)


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2. Depending on the Method, the appropriateset rate screen will be displayed. To changethe units, press the units.

3. In the Units Selection Screen select the volumeand the time base.

Flow Rate Selection

1. In the Quick Start Screen enter the flowrate(s). In this example we want to enter75 ml/min.

Press

Enter the flow rate

Press tochange the units

Press to select the maximum flowrate for the syringe size selected

Press to select the minimumflow rate for the syringe sizeselected


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Target Volume/Time Selection

1. In the Quick Start Display enter the targetvolume or time. In this example we want avolume of 60 ml.

2. Depending on the Method either Target Volume(I/W or W/I) will appear or Target Volume orTarget Time Selection (I only or W only).

3. To change the units, press the units.

4. In the Units Selection Screen select thevolume units.

For time enter h:m:s. or press again to selectsecs. Time is accurate to ±0.01secs.Remember to enter the :

Target time is only allowed in infuse only orwithdraw only Methods.

Press to select themaximum volume for the

syringe size selected

Press

Press to change the units

Press to select target time

Press to select target volume


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Previewing the Quick StartIt is simple to view the Pump Quick Start on theRUN display.

Press to view Pump Quick Start

After you have verified the configuration.Press to run the displayed configuration.

Configuring the Pump’sGeneral ParametersThe pump has basic parameters that should beconfigured during initial start-up. Once these parametersare entered they probably won’t be changed.

To enter the settings screen press

Press to previewconfiguration


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Setting the Audible Alarms

In the Settings screen , press Audible Alarms.

Select the alarms, a check mark willindicate the alarms selected.

If these alarms are selected the pumpwill provide an audible alarm.

Accept the changes made by pressingand go back to the Settings screen.

Cancel the changes made by pressingand go back to the Settings screen.

Keypad Clicks – When thekeypad is touched the unitwill provide audible feedback

Stall – Motor stalls

Press

Near End of Run – When the volume dis-pensed is reached for the specified %

Mute All – No audible sounds

End of Run –Alarm whenthe Method is finished

Power Up – Alarmwhen pump is

power cycled whilerunning


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For Near End of Run Selection

Select the % volume dispensed. For example, ifyou select 80%, the pump will sound an alarm (3short beeps) when 80% of the volume isdispensed.



Press to select %volume dispensed

For a Custom %Use this selection if the % is not listed.

Press Set Other Value to enter a custom %

Enter the percentage.



Press to entera custom %



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Locking the Touch Screen

A touch screen lock function can be enabled toprevent accidental operation or interruption ofoperation. Unlocking the touch screen requires apassword, defined by the user.

Setting the Password

To set the Password go to the Settings screen.Press the Set Password button.

Enter your desired password, up to 8 digits,and press the “ACCEPT” button.

Turning the Touch Screen Lock On

Press the Lock button twice to lock the touchscreen.

Turning the Touch Screen Lock Off

Press the “LOCK” button to bring up the Enterthe Pump Password screen.

Enter your password and press the“ACCEPT” button.

PasswordLock Button

Set password


If you forget your password contactHarvard Apparatus Technical Support at508-893-8999.

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Setting the Force LimitsThe maximum linear force of the syringe pump is 35 lbsor 16 kgs. This force is sufficient to damage delicate,low volume syringes, as well as many standard syringesif pumping viscous fluids. The % of maximum forceapplied by the pusher block can be changed.

In the settings screen, press Force.

Set the Force Level.

Select the Force Level %

Accept the changes made by pressingand go back to the Set Force screen.

Cancel the changes made by pressingand go back to the Set Force screen.

For a Custom Force %Press Set Other Value to enter a custom force %

Enter the precentage

Accept the changes made by pressingand go back to the Set Force screen.

Cancel the changes made by pressingand go back to the Set Force screen.

A minimum value of approximately 20% isrequired to overcome friction forces in themechanism. You may have to set the forcelevel to a higher value depending on theback pressure/friction in your system.

Plastic Syringes≤20ml 50% BD plastic, Sherwood,

Airtite, Terumo>20ml 100%

Stainless Steel All 100% Harvard Apparatus

Glass/Glass≤20ml 20%

Cadence (Popper)>20ml 30%

Glass/Plastic

≤1000µl 30%

Hamilton, SGE≤5ml 50%

>5ml 100%

Suggested Force Level Settings for Common Syringes

Syringe Type/Material Capacity Force Setting Common Manufacturer(s)

Press here to entercustom force %

Press


The Pump 11 Elite can accomodate syringesranging from 0.5 µl to 60 ml single syringeand 0.5 µl to 10 ml dual syringe.

The Pico Plus Elite can accomodatesyringes ranging from 0.5 µl to 10 ml.

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Setting Display BacklightThe backlight can be adjusted.

In the Settings screen, select Backlight

Select the % Backlight. For example, if you select80%, the brightness of the display will be 80% ofthe maximum.



For a Custom ValueUse this selection if the % is not listed.

Press Set Other Value to enter a custom %

Enter the percentage.



Press

Press here for customdisplay brightness level


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Setting the Pump AddressUp to 100 pumps can be linked together. Each pumpcan be given a unique address.

In the Settings Screen press Pump Address.Valid entries are 00 to 99.

Enter the pump address.



Setting the Baud RateThe Pump 11 Elite Series have an RJ-11 option for serialcommunications.

To adjust the baud rate from 9600 to 115,200 in theSettings screen press Baud Rate

Select the Baud Rate.



Press

Press

Press here toselect Baud Rate


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Power-Up RunningIf Power-Up Running is selected the pump will powerup running when power resumes.

In the Settings screen,select Power-Up Running

Accept the changes made and go backto the previous screen before SettingsMethod was selected.

Cancel and go back to the previousscreen before Settings Method wasselected.

Setting and ViewingPump InformationThe information on the pumps parameters are displayedin the Pump Information Screen.

In the Settings screen, select Pump Information

The current information on the pump will be displayed.

• The Model type

• The Software Version

• The Maintenance Due

• The Current Pump Address

Press Maintenance Reset to reset the # of hoursfor maintenance. This will reset it to 100 hours.

Confirm the Maintenance Reset by pressing theMaintenance Reset again.



Press

Press

Press here to reset themaintenance counter


This feature is only valid in Infuse Only orWithdraw Only Methods. This feature willonly execute if the pump is running at thetime when power is lost, and was runningwith no target set.

Free RTOS v6.0.1 (www.freertos.com)

NOTE: Elapsed Timers and Total InfusedVolume will be reset when power resumes.Counters and timers information prior toloss of power are lost


TiltThe touch screen can be tilted for ease of use when thepump is placed on its side.

In the Settings screen:

Press to rotate the touch screencounter-clockwise.

Press to rotate the touch screen clockwise.

Press to return the touch screen to itsoriginal orientation.

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Operating the Pump 11 Elite Series

Operating the PumpThe Pump 11 Elite Series is easy to operate because allthe pump parameters are shown on the RUN Screen.Depending on the Method of the pump, differentparameters will be shown on the display.

The Pump 11 Elite Series Run Screen is shown below.

Indicates volumedelivered and direction

Methodsettings

Total elapsed time

Total remaining time

Status of currentpump operation

Indicates currentflow direction

Total volumedelivered

Press to go toSettings screen

Fast forward

Press to Lock/Unlockthe touch screen

Fast reverse

Press to Start/Stopthe pump

Press to go toQuick Start Screen

Running the Pump

Simply press the button to start the pump.

When the pump is running the buttonis shown.

Press the button to pause the Method.

Press the to resume running the pump.

Press the to clear the counters and timers.

The fast forward and fast reverse key are notactive when the pump is running.

Press


29Operating the Pump 11 Elite Series

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Select Method

Review Method Steps(Pump Operation Profiles)

Select Syringe Manufacturer & SizeOR

Create New Syringe Type

Enter Pump Operation ProfileParameters

Validate Commands andI/O Operations

Custom Method WorkflowThe following flow diagram describes the use of aCustom Method:

The software provides the user with a variety ofprompts and warnings to help guide the setup ofMethods. For example, if a pump Method incorporatessteps that exceed the capacity of the defined syringe,the system will prompt you that parameters are out-of-range. The system also prompts users if required valuesare not entered.

The pump will check the validity ofparameters entered for each Step in theMethod, but will not review the Method inits entirety to verify that it is logical and canbe fully executed


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STEP-BY-STEP OPERATION EXAMPLE: USING ACUSTOM METHOD

The following steps describe operation using a CustomMethod.

In the following description, we are assuming the use ofa Method that is already saved on the Pump 11 EliteSeries. Note that if you make any changes to theMethod’s operating parameters and choose Accept onthe page where the changes are made, the changesautomatically become part of the stored Method forfuture use.

1. From the Method Quick Start screen, theMethod you wish to use should be displayed inthe Method Select button. If the Method is notdisplayed, select the correct Method using theprocedure outlined in Running The Pump.

2. Choose the Syringe Select button to enter theSyringe Selection screen.

3. Follow the instructions in the Syringe Selectionsection of this Guide to complete the selectionof a syringe from the built-in syringe definitionlist, or to create a new syringe definition. Whenfinished, choose the Accept button to saveyour changes, or choose the Cancel button tocancel your changes, and return to the MethodQuick Start screen.

4. Review the Pump Control Profile Stepsdescribed in the Step Definition button. Notethat the software will display the first threeSteps in the Method in the main button area.If the Method uses more than three Steps, thelast Step in the Method will be displayed in thebox that is still part of the button beneath theStep Definition button.

5. To review the Method Steps in detail or toupdate/modify any of the stored Pump ControlProfile Parameters, choose the Step Definitionbutton to enter the Method Steps SummaryScreen. The name of the Method is listed at thetop of the screen, and the complete list ofSteps is listed in a series of buttons is on theleft of the screen.

6. To review details for any individual Step in theMethod, select the Step you wish to review,then press the Step button again to enter theStep Definition screen for that Step.

7. To edit the parameters for any Pump ControlProfile, follow the instructions supplied in theapplicable Pump Operation Profile descriptioncontained in the Setting Method Parameterssection later in this Guide.

8. To return to the Method Steps Summaryscreen when finished reviewing or editingthe Step parameter details, choose theAccept button to save your changes or choosethe Cancel button to cancel any changes youmade.

9. When you are finished reviewing the operatingdetails for all Steps, choose the Back button toreturn to the Method Main screen.

10. To preview the Method, choose the PreviewMethod button, or to run the Method, choosethe Method Run button.

Step Definition Screen showing the list of Stepscurrently defined for the Method

Press to RunMethod


Configuring Custom MethodsThe Pump 11 Elite Series Programmable Models offerthe flexibility of configuring custom Methods includingmultistep Methods. The Methods and steps can also betriggered to start and stop with events and external I/O,and user defined inputs.

To determine if your pump is programmable goto the pump information screen. The Model Typeshould say Programmable.

Press

Configuring a New Custom Method

To display the Method Selectionscreen press Method.

The Method Selection screen will be displayed

Select New Method

View Methods up a page

View the Methods down a page

Views up the Method

Views down the Method

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SelectMethod

Press to see the pumpinformation screen

Press

SelectMethod


Configuring Custom MethodsThe first thing to do on a custom Method is to name theMethod.

Enter any 15 character alphanumeric name.

Accept the new name

Go back to the Method Selection screen

Select the Syringe manufacturer and Size.

To define the steps for the custom Methodpress Step Definition

Below are some predefined steps including:

Constant Rate - a single continuous rate of flow

Ramped Rate - a linearly changing flow ratebetween a starting and ending flow rate

Autofill - continuous infuse/withdraw orwithdraw/infuse operation

Gradient - combined flow streams from multiplepumps into a common output stream

In addition to these you can repeat steps or set a delaywithin a Method as well as send signals to externaldevices or react to signals received from externaldevices. This package of pumping profiles andcommands provides users with an extremely broadarray of applications that can be easily configured withcomplex external programming.

Methods can be edited and saved under a new name,simply edited to fit another application. Steps can becopied, moved, inserted or deleted.

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Setting A DelayYou can set the Pump 11 Elite Series to run after adelay. This delayed run function allows you to set up allthe parameters for operation, then have the pump startunattended after a specified amount of time.

SETTING A DELAY FOR METHODOPERATION:

1. From the Quick Start or Method Main screen,choose the Set Delay Parameters button toenter the Set Delay.

2. Enter the desired Time using the keypad.

3. If the Units of your Time are not correct foryour application, select the Units button totoggle between sec (seconds) and hh:mm:ss(hours:minutes:seconds). To use hh:mm:ssoption, enter your time value in hours,minutes, and seconds, separating each valuewith a colon. You can enter a value up to99:99:99.

4. When the desired values are entered, choose theAccept button to save your Changes and returnto the Method Quick Start screen. When a TimeDelay value is active, the Time Delay Parametersbutton is displayed in Red on the screen to alertthe user that a delay has been set.

5. Press the Run button to activate the Delay.

Set Delay Time screen

Quick Start run screen with a Time Delay Set

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Method Main

Method Selection

New Method

Name Method

Syringe Select Screen

Program Steps Summary Screen

Choose Syringe Select

Choose Step Definition

Step Definition Screen

Add Step

Complete Parameter Entry

Steps Summary Screen

Yes

Add More Steps?

Choose Accept

Creating a New Custom MethodIf none of the Methods stored on the Pump 11 EliteSeries match your requirements, you can create a newMethod and store the Method for use. The followingflow diagram outlines the basic steps you will follow tocreate a new Method:

New User-Defined Method creation workflow

TO CREATE A NEW USER-DEFINED METHOD

1. From the Method Quick Start or Method Mainscreen, choose the Method Select button toenter the Method Selection screen.

2. Select New Method then choose Accept orpress New Method again.

3. On the Method Name screen, type the name ofyour Method using the onscreen keyboard.Choose the Symbols/Numbers button todisplay the list of available numbers andsymbols that can be included in the MethodName. To switch the keyboard back to lettersMethod, choose the Letters button (ABC).

New Method Name Entry screen showing letter keys.To view numbers/symbols, choose the use button

in the lower left corner


a. Constant Rateb. Rampc. Gradientd. Autofille. Repeat

4. When you have entered the desired name foryour Method, choose the Accept button to goto the Method Main screen. Your new Methodwill now be listed in the Method Select button.

5. Choose the Syringe Select button andcomplete the description of the syringe byfollowing the procedure outlined previously inthe Syringe Selection section of this Guide.

6. To begin creating Pump Control Profile Steps,choose the Step Definition button to enter theMethod Steps Summary screen.

7. To add a Step to your Method, choose the AddStep button to access the Method Steps Menuscreen. The available options include:

8. Choose the Method Step type that you want toadd to the Method. If you choose a pumpingprofile (e.g. Constant Rate, Ramp, etc.) you willbe taken to the Step Definition screen for theselected profile.

9. Complete the parameter entry applicable to theselected Pump Operation Profile, follow thedetailed instructions supplied in the PumpOperation Profiles (Existing and User-DefinedMethods) section later in this Guide.

10. When you have completed all parameterentries on the Step Definition screen, choosethe button to save your changesand return to the Method Steps Summaryscreen.

11. Repeat items 7-10 in this procedure to includeadditional Steps in the Method.

‘ACCEPT

12. When you are done adding all required Stepsto the Method, choose the button toreturn to the Method Main screen.

‘BACK’

TO CREATE A NEW USER-DEFINED METHOD (CONTINUED)

Method Steps Profile Menu screen

f. Delayg. I/O (in) Eventh. I/O (out) Triggeri. Stop

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If you do not complete the requiredparameter entry for a Step, subsequent Stepadditions may be ignored. You should enterall required parameters for a Step whencreating it to ensure that the applicationsoftware can assemble your Methodwithout errors.

When adding a Step to a Method, the Step isadded after the currently selected Step (i.e.the Step that is highlighted in magenta)


Modifying an Existing User-DefinedMethodOnce you have created a Method or have reviewed anExisting Method, you may have a need to modify thestructure of the Method (i.e. the types of Steps andCommands included, not just changes in the operatingparameters for a specific Step. The followingprocedures will help you to efficiently modify a storedMethod.

CHANGING THE ORDER OF STEPS IN A METHOD

1. From the Method Main screen, choose theStep Definition button to enter the MethodSteps Summary screen.

2. Highlight (select with a single touch) the Stepfor which you want to change the order.

3. Choose the Move Step Up or Move Step Downbutton, as required, to move the Step into theposition that you want it executed.

DELETING A STEP FROM A METHOD

1. From the Method Main screen, choosethe Step Definition button to enter the MethodSteps Summary screen.

2. Highlight (select with a single touch) the Stepthat you want to delete.

3. Choose the Delete Step button. The applicationwill display a warning message in the MessageArea asking you to confirm your deletionrequest.

4. To confirm the deletion, choose the DeleteStep button again.

Defining Method Step ParametersThis section of the User Guide describes each of thefunctions available for use in Pump 11 Elite SeriesExisting and User-Defined Methods. The information inthis section is designed to help you set the individualparameter values in each of the Method Step optionsand to properly use the Advanced Options commands.

The information that follows is organized into twosections:

• Pump Operation Profiles

• Advanced Options (Commands and I/O)

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PUMP OPERATION PROFILES

The Pump 11 Elite Series Existing Methods and all User-Defined Methods use Pump Operation Profiles as partof the Methods controlling the pump. These profiles arecombined with a syringe selection and any Commandsor Input/Output triggers to create a complete Method.The following sections describe each profile along withinstructions for setting up the profile. The followingPump Operation Profiles are available on the Pump 11Elite Series:

CONSTANT RATE

GRADIENT AUTOFILL

RAMP RATE

FlowRate

Time | Volume

FlowRate

Time# Cycles

Pump Operation Profiles available on the Pump 11 Elite Series

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Flow Rate

Withdraw

Infuse

Time | Volume

# Cyclesor TotalVolume

% ofFlowRate

Time


FlowRate

Time | Volume

STOP

Target Timeor TotalVolume Point

Constant Rate ProfileThe Constant Rate profile is used to run the pump at adefined flow rate and for a specific pumping mode(infuse or withdraw) up to a target volume or for adefined time period.

Constant Rate Pump Operation Profile

When creating or editing a Constant Rate profile, youwill define three primary operating parameters:

• The Flow Method defining the direction offlow: infuse or withdraw

• The Flow Rate to define the rate of uptake ordispensing

• The Target Volume or Run Time that determinehow long the pump will operate at the set rate

When creating or editing a Constant Rate profile, youcan set the rate in ml, µl, nl or pl units of volume usingsec, min or hr as the units of time. The systemautomatically calculates a minimum and maximum flowrate based on the syringe selected or custom-defined.The min/max values are made available as buttonselection options on the Set Infuse and Withdraw Ratescreens.

Constant Rate Profile Setup Screen

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TO SET UP/EDIT A CONSTANTRATE PROFILE:

1. From the Step Definition screen for a ConstantRate profile, choose the Set Mode button totoggle the Flow Method between Infuse andWithdraw operation.

2. Choose the Set Rate button to enter the SetRate screen.

3. Enter the flow rate value desired using thekeypad or select the Minimum Rate orMaximum Rate button.

4. If the Units of Rate are not correct for yourapplication, choose the Units button.

5. On the Units Selection screen, select the unitof measure (ml, µl, nl or pl) and the unit oftime(sec, min, hr) as appropriate, then choose

to confirm your selections.

6. When all parameters are correctly set on theSet Rate screen, choose to confirmyour settings and return to Constant RateDefinition screen.

7. Choose the Set Target Volume or Time buttonto enter the Set Volume or Time screen.

8. On the Set Volume or Time screen, select theVolume or Time button, as desired.

9. Enter the desired value using the keypad, orselect the Maximum Volume button.

10. If the Units of Volume/Time are not correct foryour application, choose the Units button tothe right of the parameter entry box. For theUnits of Volume, this choice takes you to theUnits Selection screen. For the Units of Timeoption, this choice will toggle the buttonbetween sec (seconds) and h:m:s(hours:minutes:seconds). To use the h:m:soption, enter your time value in hours, minutesand seconds, separating each value with acolon.

11. If you are changing the Units of Volume, on theUnits Selection screen, select the unit ofmeasure (ml, µl, nl or pl) as appropriate, thenchoose to confirm your selection.

12. When all parameters are correctly set on theSet Target Volume or Time screen, choose

to confirm your settings and returnto the Constant Rate Definition screen.

13. Choose again to return to the StepDefinition screen.

‘ACCEPT’

‘ACCEPT’

‘ACCEPT’

‘ACCEPT’

‘ACCEPT’


FlowRate

Time

STOP

Target Time

Ramp ProfileThe Ramp Rate profile is used to create a linearlyincreasing or decreasing flow rate for a single pumpingMethod (infuse or withdraw) over the course of adefined time period.

Ramp Rate Pump Operation Profile

When creating or editing a Ramp Rate profile, you willdefine three primary operating parameters:

• The Flow Method defining the direction offlow: infuse or withdrawl

• The Flow Rate at the beginning and end ofthe pumping cycle

• The Target Time that determines the totallength of time over which the ramp will becalculated

During a Ramp Rate profile, the Pump 11 Elite Seriesvaries the flow rate linearly between the starting rateand ending rate over the full time period entered. ARamp Rate profile can be applied to an increasing ordecreasing rate application.

When creating or editing a Ramp Rate profile, you canset the rate in ml, µl, nl or pl units of volume using secor h:m:s (hours:minutes:seconds) as the units of time.The system automatically calculates a minimum andmaximum flow rate based on the syringe selected orcustom-defined. The min/max values are made availableas button selection options on the Set Infuse andWithdraw Rate screens.

TO SET UP/EDIT A RAMP RATE PROFILE:

1. From the Step Definition screen for a RampRate profile, choose the Set Mode button totoggle the Flow Method between Infuse andWithdraw operation.

2. Choose the Set Start and End Rate button toenter the Set Start & End Rates screen.

3. Select the Set Start and End Rate button(it should be selected by default as indicatedby the active data entry box to the right of thebutton). Enter the flow rate value desired usingthe keypad or select the Minimum Rate orMaximum Rate button.


5. On the Units Selection screen, select the unitof measure (ml, µl, nl or pl) and the unit oftime (sec, min, hr) as appropriate, then choose

to confirm your selections.

6. Repeat Steps 3 to 5 for the End Rate byselecting the End Rate button and continuingdata entry.

7. When all parameters are correctly set on theSet Rate screen, choose to confirmyour settings and return to Ramp Definitionscreen.

8. Choose the Time button to enter the Set Timescreen.

9. Enter the desired Time value using the keypad.

10. If the Units of Time are not correct for yourapplication, select the Units button to togglebetween sec (seconds) and h:m:s(hours:minutes:seconds). To use the h:m:soption, enter your time value in hours, minutesand seconds, separating each value with acolon.

11. When all parameters are correctly set on theSet Target Time screen, choose Accept toconfirm your settings and return to the RampDefinition screen.

12. Choose Accept again to return to the StepDefinition screen.

‘ACCEPT’

‘ACCEPT’

Step Definition screen for a Ramp Rate profile

Set Start and End Rates screen

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Ramps ≤ 2 seconds from min to max ratesmay cause motor stalls.


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Gradient ProfileThe Gradient profile is used to combine multiple flowstreams from different pumps into a common outputstream. The flow profiles can be constant, rampedor stepped.

TO SET UP/EDIT A GRADIENT PROFILE:

1. From the Step Definition screen for Gradient,choose Set Combined Flow Rate to enter theSet Rate screen.

When creating or editing a Gradient profile you willdefine 4 primary operating parameters:

• The combined Flow Rate

• The Target Starting and Ending Percentagesfor each pump in the system.

• The Target Time that determines the totallength of time over which the Gradient profilewill be calculated

• The Number of Steps that will be created inthe profile

2. Enter the desired combined flow rate value forboth pumps using the keypad or select theMinimum Rate or Maximum Rate button.


4. On the Units Selection screen, select the unitof measure (ml, µl, nl, or pl) and the unit oftime (sec, min, hr) as appropriate, then chooseAccept to confirm your selections.

5. When all parameters are correctly set on theSet Rate screen, choose Accept to confirmyour settings and return to the Step Definitionscreen.

6. Choose the Set Percentage button for pump 00.

7. Set the Start and End Percentages for pump 00then choose Accept.

8. Choose the Address button, and then set theaddress of the second pump. Choose Accept toreturn to the Step Definition screen.

!CAUTION: ALL PUMPS MUST USETHE SAME SYRINGE SIZE ANDTYPE. IF THERE IS A SYRINGECHANGE AT MASTER THEN SAMECHANGE MUST OCCUR AT ALLSATELLITES.

% ofFlowRate

Time

See Appendix C: RS-485 for physicalgradient connections

Master address must be set to 00. Ensurethat the satellite’s address matches theaddress set in the gradient step.

Make all cable connections, then power-upthe satellite pump before powering-up themaster pump.


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9. The pump will calculate the remainingpercentages for the second pump.

10. Choose the Set Time and # Steps button. Enterthe total amount of time and the number ofsteps desired and then choose Accept.

Running a Gradient ProfileThe pump control button’s background color indicatesthe status of the satellite unit.

Blue Background= Connected / Motor Stopped

Red Background= Comm Error

Yellow Background= Motor Stall

Green Background= Motor Running

Pump Controlbuttonshowing thesatellite’saddress

For a Constant rate or Ramp profilechoose 0 steps. For a Steppedprofile select the number of stepsdesired.

When running a Gradient step, the satellitepump’s screen will display “Satellite”followed by its pump address.A stop buttonwill be active on the satellite when thepump is running.

When running a gradient step, ensurethat no unused pumps are included in thepump chain.


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Autofill Cycling ProfileThe Autofill Cycling Profile is an automatedInfuse/Withdraw or Withdraw/Infuse step that operatesat constant, but separately defined, flow rates for thewithdraw and infuse operations. The Autofill profile isdesigned to work in conjunction with an autofill valvethat controls the switching of flows from a reservoir forrefilling the syringe, to the target infusion subject.

Flow Rate

Withdraw

Infuse

Time | Volume

# Cyclesor TotalVolume

When creating or editing an Autofill Cycling profile, youwill define the following operating parameters:

• The Flow Mode defining the cycle:Infuse/Withdraw (INF/WD) or Withdraw/Infuse(WD/INF)

• The Flow Rates to be used duringInfuse/Withdraw operations

• The infusion volume to be delivered duringeach pump cycle

• The total volume to be delivered during allcycles or the total number of Infuse/Withdrawor Withdraw/Infuse cycles that you want thepump to execute. For example, if you want topump 100 ml using a 10 ml syringe, the pumpwill execute 10 Infuse/Withdraw orWithdraw/Infuse cycles, depending upon thechosen flow mode.

During an Autofill profile, the Pump 11 Elite operates ata constant pump rate during the infusion and withdrawalstages, although the rate can be different for eachdirection (i.e. infuse vs. withdraw).

When creating or editing an Autofill Cycling profile, youcan set the rate in ml, µl, nl or pl units of volume usingsec, min or hr as the units of time. The systemautomatically calculates a minimum and maximum flowrate based on the syringe selected or custom-defined.The min/max values are made available as buttonselection options on the Set Rate and Concentrationscreen.


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TO SET UP/EDIT AN AUTOFILL CYCLING PROFILE:

1. From the Step Definition screen for an AutofillCycle, choose the Set Mode button to togglebetween the Withdraw/Infuse (WD/INF) andInfuse/Withdraw (INF/WD) modes.Withdraw/Infuse begins by withdrawing intothe syringe, following by infusing into thetarget.

2. Choose the Set Rates button to enter the SetInfuse/Withdraw Rate screen.

3. Select the Infuse Rate (it should be selected bydefault as indicated by the active data entrybox to the right of the button). Enter thedesired rate using the keypad or select theMinimum Rate or Maximum Rate button.


5. On the Units Selection screen, select the unitof measure (ml, µl, nl or pl) and the unit oftime (sec, min, hr) as appropriate, then chooseAccept to confirm your selections.

6. Select the Withdraw Rate and enter the desiredrate using the keypad or select the MinimumRate or Maximum Rate button.

7. When all parameters are correctly set on theSet Infuse/Withdraw Rate screen, chooseAccept to confirm your settings and return toStep Definition screen.

8. Choose the Set Volume per Cycle button toenter the Set Volume per Cycle screen.

9. Enter the Volume using the keypad.

10. If the Units of Measure associated with Volumeare not correct for your application, choose theUnits button to enter the Units selectionscreen. Select the unit of measure button (ml,µl, nl, pl) appropriate for your application, thenchoose the Accept button to return to the SetTarget Volumes screen.

11. When all parameters on the Set Volume per

Cycle screen are correct, choose the Acceptbutton to return to the Step Definition screen.

12. Choose the Set Total Volume or # Cyclesbutton to enter the Set Total Volume or #Cycles screen.

13. Select either Total Volume or # Cycles to definehow the pump will control pumping activity.Total Volume will cycle the pump the correctnumber of times to deliver the total volumeaccording to the Volume per Cycle setting. Forexample, if you set a 10 ml Volume per Cycleand set 100 ml in Total Volume, the pump willcycle 10 times.

14. If you select Total Volume, enter the volumedesired using the keypad. If you select #Cycles, enter the total number ofInfuse/Withdraw or Withdraw/Infuse cyclesthat you wish to perform.

15. If the Units of Measure associated with Volumeare not correct for your application, choose theUnits button to enter the Units selectionscreen. Select the unit of measure button (ml,µl, nl, pl) appropriate for your application, thenchoose the Accept button to return to the SetTarget Volumes screen.

16. When all parameters on the Set Total Volumeor # Cycles screen are correct, choose theAccept button to return to the Step Definitionscreen.

Incorporating Advanced Options(Commands and I/O) Into A MethodPump 11 Elite Series Methods can incorporate a varietyof commands that expand the versatility of the systemby permitting convenient looping, chaining of events,sending signals to external devices, or acceptinginbound signals from external devices. These AdvancedOptions controls are described in this section of theGuide.

Using maximum rate may not be appropriateduring the Withdraw stage of an Autofillprofile, since air can be drawn into thesyringe, or a high vacuum pressure can beproduced depending on the syringe and/ortubing being used, or the fluid viscosity.


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TO INSERT A STEP REPEAT SEQUENCE IN AMETHOD:

1. From the Method Main screen, choose StepDefinition to enter the Step Definition screen.

2. Highlight (select once) the Method Step thatyou want the repeat step to follow.

3. Choose the button to access theMethod Steps Menu screen.

4. Choose Repeat to insert the Repeat Stepfunction into the Method.

5. Select the Step(s) you would like to repeatfrom by selecting the Step button twice orselecting once and then choosing Accept.

‘ADD STEP’

Advanced Options Repeat Step screen. Choosing Repeatbrings you to a listing of the available steps in the Method

StopThe Stop Command is used to instruct the pump to stopoperation. This Command can be used in conjunction with I/OEvents to control pump operation based on external events.

TO INSERT A STOP COMMAND IN A METHOD:

1. From the Method Main screen, chooseStep Definition to enter the Step Definitionscreen.

2. Highlight (select once) the Method Step that youwant the Stop Command to follow.


4. Choose Stop to insert the Stop Command intothe Method.

‘ADD STEP’

6. Enter the number of repeats using the keypadthen choose the Accept button to save yourchange and return to the Step Definition screen.

Advanced Options Set Delay Time screen

5. Enter the desired Time using the keypad.

6. If the Units of Time associated with Time are notcorrect for your application, select the Units buttonto toggle between sec (seconds) [0.2 secondsminimum allowable value] and h:m:s(hours:minutes:seconds). To use the h:m:s option,enter your time value in hours, minutes andseconds, separating each value with a colon. Youcan enter a value up to 99:99:99.

7. When your Time entry is correct, choose the Acceptbutton to insert the Delay Step in the Method andreturn to the Step Definition screen.

Repeat StepThe Repeat Command is used to repeat Steps in a procedurea defined number of times. To use this Command, you will tellthe application what Steps you want to Repeat and how manytimes you want them repeated. The Repeat Step commandcreates a “jump” back to a specific Step in a Method thatexecutes the defined Step plus all following Steps up to theRepeat command (i.e. it creates a Method loop).

When defining a Repeat jump in a Method, the Commandtargets the Step sequence position and not the function thatcurrently occupies that position. For example, if you create aRepeat jump to Step 4 in a Method (currently a Ramp PumpOperation Profile) and subsequently move a Constant Rate profileinto the Step 4 position, the Repeat jump will then connect to theConstant Rate operation and not to the Ramp operation.

DelayThe Delay Command is used to insert a time delay in aprocedure. You can set a time delay between 0.2seconds up to 99:99:99 (99 hours, 99 minutes, 99seconds).

TO INSERT A TIME DELAY IN A METHOD:


2. Highlight (select once) the Method Step thatyou want the time delay to follow.

3. Choose the Insert Step button to access theMethod Steps Menu screen.

4. Choose Delay to enter the Set Delay Time screen.

This command creates a loopback to thedesignated Step in a Method, executing theselected Step, plus all subsequent Steps upto the point of insertion of the Repeatcommand. For example, if the Repeatcommand is set to repeat Step 2 one time, thesystem will loop back to Step 2 and executeboth Steps 2 and 3 one additional time.


I/O EVENT TRIGGERS

The Pump 11 Elite Series pump permits easyconnection to external I/O devices via a variety ofinterfaces, including a built-in 15-pin D-sub connector.The Pump 11 Elite Series software incorporates simplecommands for incorporating I/O controls into your pumpMethods. General trigger signals are integrated in thesoftware. The following procedure describes the use ofthe Advanced Options I/O commands. Following theprocedure is a description of the selection menu optionsfor each of the I/O command types. Details for theelectrical connection controls and pin-out description forthe 15-pin User I/O Connector are provided in AppendixC of this manual.

The Pump 11 Elite Series manages trigger eventsthrough specific pins on the I/O Connector port on theback of the pump. The following details define pin logic:

• Trigger is communicated via Pin 4.

• Event signal are communicated via Pin 2.

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I/O (OUT) TriggerThe I/O (OUT) Trigger command is used to send asignal to an external device when the Step in theMethod is reached.

I/O (OUT) Trigger Advanced Options menu

TO INSERT AN I/O (OUT) TRIGGER COMMAND:


2. Highlight (select once) the Method Step thatyou want the I/O (OUT) Trigger to follow.


4. Choose the I/O (OUT) Trigger button to enterthe Trigger Step Definition Screen.

5. Set your Trigger settings by using thefollowing procedure:

a. To send a Trigger signal to an externaldevice via Pin 4, select the Trigger 1button. Then select whether the signalwill be High or Low by selecting theappropriate button (selection willhighlight in white).

6. Choose the Accept button to save yourchanges and return to the Step Definitionscreen.

‘ADD STEP’

The following option is available when setting an I/O(OUT) Trigger command:

• Trigger 1: Specifies output via the Trigger 1(Pin 4) port. When selected, you must alsospecify whether the signal to be sent is HighSignal or Low Signal.

– High Signal: Sets the output signal tologic high.

– Low Signal: Sets the output signal tologic low.

When using triggers, the first step in yourMethod should define the trigger startinglevel (High or Low). You may then use thetrigger command to change the level asdesired for your Method.


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I/O (IN) EventThe I/O (IN) Event command is used to have the pumppause and wait for a signal from an external device orfor a manual user input at the touch screen. Theseexternal signals are communicated via Pin 2 on the15-pin I/O Connector port.

The following options are available when setting an I/O(IN) Event command.

• Input High-to-Low: Sets the pump to wait fora logic low input signal falling edge.

• Input Low-to-High: Sets the pump to wait fora logic high input signal rising edge.

• User Press: Sets the pump to wait for manualinput from the user at the touch screen(screen touch).

TO INSERT AN I/O (IN) EVENT COMMAND:


2. Highlight (select once) the Method Step thatyou want the I/O (IN) Event to follow.


4. Choose the I/O (IN) Event button to enter theEvent Step Definition Screen.

5. Select either Input High, Input Low or UserPress, as appropriate.

6. Choose the Accept button to save yourchanges and return to the Step Definitionscreen.

‘ADD STEP’


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Saving A Copy Of A MethodFollow these instructions to save a copy of a Methodunder a new name:

1. From Quick Start screen, choose the MethodSelect button to enter the Method Selectionscreen.

2. From the list of available Methods, select(press once) the Method you want to save acopy of, then choose the File Options button toenter the File Options screen.

3. Choose Save As/Copy to access the MethodName Entry screen. The current Method Namewill be displayed in the text entry box.

4. On the Method Name screen, type the newname for your Method using the onscreenkeyboard. Choose the Symbols/Numbersbutton (.?123) to display the list of availablenumbers and symbols that can be included inthe Method Name. To switch the keyboardback to letters Method, choose the Lettersbutton (ABC). [15 characters maximum]

5. When finished entering the new MethodName, choose Accept to save your changesand return to the File Options screen.

6. Choose to return to the MethodSelection screen.

Renaming A MethodFollow these instructions to rename a previously savedMethod:

1. From the Quick Start screen, choose theMethod Select button to enter the Methodselection screen.

2. From the list of available Methods, select(press once) the Method you want to rename,then choose the File Options button to enterthe File Options screen.

‘BACK’

3. Choose Rename Method to access the MethodName Entry screen. The current Method Namewill be displayed in the text entry box.

4. On the Method Name screen, type the newname for your Method using the onscreenkeyboard. Choose the Symbols/Numbersbutton (.?123) to display the list of availablenumbers and symbols that can be included inthe Method Name. To switch the keyboardback to letters Method, choose the Lettersbutton (ABC). [15 characters maximum]

5. When finished entering the new MethodName, choose Accept to save your changesand return to the File Options screen.


Appending A MethodFollow these instructions to Append a previouslycreated Method into a new Method:

1. From the Method Main or Quick Start screen,choose the Method Select button to enter theMethod Selection screen.

2. From the list of available Methods, select(press once) the Method you want as the baseMethod.

3. Choose the File Options button to enter the FileOptions screen.

4. Choose Append to access the Method toAppend screen.

5. Choose (press twice) the Method you wouldlike to Append (attach) to your base Method.The pump will return you to the File Optionsscreen and display a confirmation that theMethod was copied.


Deleting A MethodFollow these instructions to delete a previously savedMethod:

1. From the Method Main or Quick Start screen,choose the Method Name button to enter theMethod Selection screen.

2. From the list of available Methods, select(press once) the Method you want to delete,then choose the File Options button to enterthe File Options screen.

3. Choose Delete Method. The application willdisplay a warning message in the MessageArea asking you to confirm your deletionrequest.

4. Choose Delete Method again to confirm thedeletion and return to the Method Selectionscreen.

‘BACK’

‘BACK’

MANAGING PUMP 11 ELITESERIES METHODS

All Existing and User-Defined Methods are stored in theMethod List for ongoing use. Over time, you may wantto manage previously created Methods to keep yourMethod List easy to navigate. In addition, the Pump 11Elite Series allows you to export Method information toan externally connected pump or to a computer that willbe used to control operation. This section of the Guidedescribes the file management facilities available on thePump 11 Elite Series.

The Method management proceduresoutlined in this section are not available forQuick Start Methods (Infuse only, Withdrawonly, I/W or W/I)


PUMP 11 ELITE SERIES METHODEXPORT AND IMPORT

The Pump 11 Elite Series pump permits the daisy chainingof multiple pumps, as well as integration with/control byan external computer through HyperTerminal. To facilitiateoperation in these Methods, the Pump 11 Elite Seriesapplication software includes a variety of commandsdesigned to simplify the export/import of Methodsbetween the pump and external devices. This sectionoutlines the use of these commands.

TRANSFER A METHOD BETWEEN PUMPS

Follow these instructions to transfer a Method betweentwo Pump 11 Elite Series pumps.

1. Connect two pumps together via a RS-485(firewire type) cable and set the address on thefirst pump to ‘00’ using the Set Pump Addresscommand in the Settings menu.

2. Set the address on the second pump tosomething other than ‘00’ using the Set PumpAddress command in the Settings menu.

3. From the Method Main screen, choose MethodSelect then choose the Method you would liketo export.

4. Choose the File Options button.

5. Choose the Pump button and enter the addressfor the pump you would like to receive theMethod.

6. Choose Export Method to Pump.

7. The pump will display a confirmation messagein the Message Area on the screen when theexport is complete. Note that long Methodsmay take a few seconds to export.

8. Before disconnecting the pumps, verify thatthe Method was successfully exported bychoosing Method Select from the MethodMain screen on the destination pump, thenbrowse the available Methods to verify that theexported Method is shown in the menu.

Method option screen

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Import will only work when the destinationpump has 0 or 1 Method stored.

Do not attempt to Export/Import Methodswhile the pump is running.

Do not change screens whileImporting/Exporting a Method.

For additional details on the physicalinterfaces used to connect the Pump 11 EliteSeries to external devices, refer to theExternal I/O Connections section in theAppendices.


EXPORTING A METHODTO AN EXTERNAL COMPUTER

Follow these instructions to export a Method to anexternal computer.

1. Connect the pump to the external computer viaUSB cable.

2. On the computer, open the HyperTerminalapplication and verify that the computer cancommunicate with the pump.

3. At the pump, from the Method Main screen,choose Method Select then choose the Methodyou would like to export.

4. Choose the button.

5. In HyperTerminal, choose Transfer CaptureText...

6. Enter the File Name that you want to savethe Method under, then choose Start.

7. Choose Export Method to PC.

8. The pump will display a confirmation messagein the Message Area on the screen when theexport is complete. Note that long Methodsmay take a few seconds to export.

9. At the destination computer, in HyperTerminal,when the Method is done transferring, themessage “mend” will appear onscreen.

10. In HyperTerminal, choose Transfer CaptureText... Stop. Your Method is now saved on theexternal computer.

‘FILE OPTION’

IMPORTING A METHOD FROM AN EXTERNALCOMPUTER

Follow these instructions to import a Method from anexternal computer.

1. Connect the pump to the external computer viaa USB cable and set the Address value to ‘00’using the Set Pump Address command in theSettings menu.

2. On the computer, open the HyperTerminalapplication and verify that the computer cancommunicate with the pump.

3. In HyperTerminal, click on the Properties iconsand choose the Settings tab on the pop-up boxthat is displayed.

4. Click on the ASCII Setup button.

5. In the Line Delay field, enter 500. This will set a500 millisecond delay after each line istransferred during the import process.

6. Click OK twice to return to the HyperTerminalMain Menu screen.

7. In HyperTerminal, choose Transfer Send TextFile...

8. Select the Method that you want to import tothe pump and click Open.

9. Before disconnecting, verify that the Methodwas transferred by choosing Method Selectfrom the Method Main screen at thedestination pump, then browse the Methodmenu to confirm that the new Method is nowsaved at the pump.

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EXTERNAL PUMP CONTROL

This section of the Pump 11 Elite Series manualdescribes the control of the pump using an externalcomputer device. If communicating via the USB Port,see Appendix C for one time installation instructions ofUSB Driver.

Setting Up HyperTerminalHyperTerminal is a Windows application designed tosupport the external control of devices such as thePump 11 Elite Series pump through a USB connection.The following instructions describe the configuration ofthe HyperTerminal application.

1. Select “Start – All Methods – Accessories –Communications – HyperTerminal”.

2. Enter a name for a New Connection(i.e Pump_11_Elite), then click OK.

4. Set up the Port Settings as shown below andclick OK.

3. Select the Virtual Comm Port from the“Connect using” drop-down list. Click OK. (Ifthe Virtual Comm Port is not known, useDevice Manager to find it. Instructions onsetting up the Virtual CommPort Driverare supplied in Appendix E.)

5. Verify the Settings are as shown below.

6. Choose ASCII Setup and select “Echo typedcharacters locally”then click OK to completethe setup.

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To determine the proper comport, in yourPCs “Systems Properties” screen, under the“Hardware” tab, select “Device Manger”.Expand the “ports (COM & LPT)” sectionand you should see “Syringe Pump”. Thecomport is listed next to this in parenthesis.

NOTE: To determine if your pump isconnected correctly, in Hyperterminal’smain window press ‘Enter’; a ‘:’ shouldappear. If not connected properly, closeHyperterminal, turn off the pump, unplugthen re-plug the USB cable, turn on thepump and re-open Hyperterminal.


PUMP CHAIN COMMANDS

The Pump Chain commands allow all pump controlinformation to be managed from an external computersource. These commands can also be used to control aseries of pumps (up to 100) from a single computerinterface.

Pump 11 Elite Series commands are communicated tothe pump via a USB port interface through a terminalMethod such as Harvard Apparatus’ Method Manageror HyperTerminal. In using the Pump Chain commands,you will need to assign each pump in the pump chain aunique address, using the Set Pump Address commandin the Settings menu. The address range is from 00 to99. This address value is used to identify which pump isto receive a command and which pump is responding.The first pump in the chain, the one connected to thecomputer, must use address 0 (zero). Configure eachpump with its assigned address and baud rate asdescribed in the Pump Settings section of this guide.

Using the Pump 11 Elite Series PumpChain CommandsThe following instructions will help you to utilize thePump Chain commands feature on the Pump 11 EliteSeries pump.

Commands may be abbreviated to the first four letters,i.e. address would be abbreviated addr. A space mustfollow the command if arguments are included.

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In the command list below, the following convention isused:

Required parameter

[ ] Optional parameter

| Separator between parameterchoices

# Numeric value without precedingzeros

### Numeric value with preceding zeros

#-# A range of values

<cr> Carriage return

<lf> Line feed

<sp> Space

[prefix] Pump address prefix in the format#: if the pump address is not zero

<prompt> Prompt (see below)

The following prompts are returned after a command isexecuted:

: The pump is idle

> The pump is infusing

< The pump is withdrawing

* The pump stalled

T* The target was reached

If the pump is in poll mode, an XON character is addedafter the prompt.

If the pump address is nonzero, the pump address isprefixed to the prompt without a colon.

In the following list of commands:

• The pump address is prefixed to everyresponse line followed by a colon.

• <lf>[##]<prompt> is the response unlessotherwise noted.

If the pump address is nonzero, the one or two-digitpump address precedes the command. For example, toset the infuse rate for pump 12, the command wouldlook like “12irat 3.2 u/m”.

Every line of the response from a pump with a nonzeroaddress is prefixed by the two digit pump address(prefixed with a 0 if necessary) followed by a colon. Nocolon is used to seperate the pump address from theprompt however.

NOTE: When daisy-chaining using theoptional RJ-11 connectors, all pumps mustuse the same baud rate. Values above 38400are recommended.

If the USB port is used on a pump with anonzero address, the pump address does notneed to be used.

NOTE: If you are using the optional RJ-11connections for daisy-chaining, theconnection from the computer must alsocome through the RJ-11 connectors.

NOTE: Once communication is established,if you manipulate the touch screen, youmust reestablish communications to the PC(<CR> will do this)

NOTE: System commands and start/stopcommands can be executed from Method orMethod configuration screens. However, forparameter commands (rate, dia, vol, etc) it isnecessary to execute from the Quick Startconfiguration screen.


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Error MessagesError messages are displayed if the entered commandcannot be executed for some reason. The error messagewill take up two lines with the first line being themessage type and the second line describing the erroritself. The second line may be up to 80 characters long.

Command ErrorsCommand errors are displayed when the command isunrecognized, entered in the wrong mode, or the stateof the pump keeps the command from executing (i.e.using the IRUN command if a limit switch is active).

The command error has the following format:

<lf>[##:]Command error:<cr>

<lf>[##:]<sp><sp><sp>error message<cr>

<lf>[##]<prompt>

Argument ErrorsArgument errors are displayed when a commandargument is unrecognized or out of range. The argumentin question will be displayed except in the case ofmissing arguments.

The argument error has the following format:

<lf>[##:]Argument error: [bad argument]<cr>

<lf>[##:]<sp><sp><sp>error message<cr>

<lf>[##]<prompt>

System commands

ADDRESSSets or displays the pump address. Valid range is 0 to99.

Command format:

address [0-99]

Query response:

<lf>[##:]Pump address is #<cr>

<lf>[##]<prompt>

Notes: Pumps with an address of 0 are masters, andpumps with an address between 1 and 99 are slaves.

BAUDSets or displays the baud rate. Valid baud rates are9600, 19200, 38400, 57600, 115200, 128000, 230400,256000, 460800, and 921600. Note that somecomputers may not be able to handle baud rates above115200.

Command format:

baud [9600 | 19200 | 38400 | 57600 | 115200 |128000 | 230400 | 256000 | 460800 | 921600]

Query response:

<lf>[##:]# baud<cr>

<lf>[##]<prompt>

CATALOGDisplays a catalog of Methods stored in the pump.

Command format:

cat

Query response:

<lf>[##:]Method name Size<cr>

<lf>[##:]--------------- ----<cr>

<lf>[##:]Method name #<cr> This line isrepeated for each file on the disk

<lf><lf>[##:]# file(s) using # steps<cr>

<lf>[##]<prompt>

Notes: The file size is the number of steps in theMethod plus one for the file information header.

The last line shows the number of files and the totalnumber of steps being used on the disk.

DELMETHODDeletes a Method stored in the pump.

Command format:

delMethod Method

DIMSets or displays the backlight level in percent. Validrange is 0 to 100. Note that specifying 0 will turn thebacklight off so that the screen will not be visible.

Command format:

dim [0-100]

Query response:

<lf>[##:]Backlight is set to #%<cr>

<lf>[##]<prompt>


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ECHOSets or displays the USB echo state. Valid states are onor off.

Command format:

echo [on|off]

Query response:

<lf>[##:]Echo is OFF<cr>

<lf>[##]<prompt>

or:

<lf>[##:]Echo is ON<cr>

<lf>[##]<prompt>

FORCESets or displays the infusion force level in percent. Validrange is 1 to 100.

Command format:

force [1-100]

Query response:

<lf>[##:]#%<cr>

<lf>[##]<prompt>

METRICSDisplays the pump metrics. The operation of thiscommand is covered in the 11 Elite Configurationdocument.

Command format:

metrics

POLLSets or displays the polling mode state. In polling mode,prompts are inhibited if they aren’t generated by a typedcommand. Valid states are on or off.

Command format:

poll [on|off]

Query response:

<lf>[##:]Polling mode is OFF<cr>

<lf>[##]<prompt>

or:

<lf>[##:]Polling mode is ON<cr>

<lf>[##]<prompt>

TIMESets or displays the date and time.

Command format:

time [mm/dd/yy] [hh:mm:ss]*

Query response:

<lf>[##:]##/##/## ##:##:## #M<cr>

<lf>[##]<prompt>

* 24 hour time format

VER

Displays the short version string.

Command format:

ver

Query response:

<lf>[##:] 11 Elite #.#.#<cr>

<lf>[##]<prompt>

VERSIONDisplays the full version string.

Command format:

version

Query response:

<lf>[##:]Firmware: v#.#.#<cr>

<lf>[##:]RTOS: FreeRTOS v#.#.#(http://www.freertos.org)<cr>

<lf>[##:]Pump address: #<cr>

<lf>[##]<prompt>


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Run commands

IRUNRuns the pump in the infuse direction. Quick Start modeonly.

Command format:

irun

rrun

Runs the pump in the opposite direction. Quick Startmode only.

Command format:

rrun

RUNSimulates a key press of the run button located in thelower right corner of the screen.

Command format:

run

STOP / STPStops the pump.

Command format:

stop

stp

WRUNRuns the pump in the withdraw direction. Quick Startmode only.

Command format:

wrun

Rate commands

CRATEDisplays the current rate that the motor is running at. Avalid response is returned only in dynamic situations(while the pump is running). Quick Start mode only.

Command format:

crate

Query response:

<lf>[##:]Infusing at # xl/xxx<cr>

<lf>[##]<prompt>

or:

<lf>[##:]Withdrawing at # xl/xxx<cr>

<lf>[##]<prompt>

DIAMETERSets or displays the syringe diameter in mm. Quick Startmode only.

Command format:

diameter [syringe diameter]

Query response:

<lf>[##:]#.#### mm<cr>

<lf>[##]<prompt>

IRAMPSets or displays the target infusion rate while ramping.Quick Start mode only.

Command format:

iramp [start rate start units end rate end unitsramp time in seconds]

Query response:

<lf>[##:]Ramp not set up.<cr>

<lf>[##]<prompt>

or:

<lf>[##:]Starting rate: # xl/xxx<cr>

<lf>[##:]Ending rate: # xl/xxx<cr>

<lf>[##:]Ramp time: # seconds<cr>

<lf>[##]<prompt>

IRATESets or displays the infusion rate. Quick Start modeonly.

Command format:

irate [rate rate units] [max | min]

Query response:

<lf>[##:]# xl/xxx<cr>

<lf>[##]<prompt>


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WRAMPSets or displays the target withdraw rate while ramping.Quick Start mode only.

Command format:

wramp [start rate end rate rate units ramptime in seconds]

Query response:

<lf>[##:]Ramp not set up.<cr>

<lf>[##]<prompt>

or:

<lf>[##:]Starting rate: # xl/xxx<cr>

<lf>[##:]Ending rate: # xl/xxx<cr>

<lf>[##:]Ramp time: # seconds<cr>

<lf>[##]<prompt>

WRATESets or displays the withdraw rate. Quick Start modeonly.

Command format:

wrate [rate rate units] [max | min]

Query response:

<lf>[##:]# xl/xxx<cr>

<lf>[##]<prompt>

Volume commands

CIVOLUMEClears the infused volume. Quick Start mode only.

Command format:

civolume

CTVOLUMEClears the target volume. Quick Start mode only.

Command format:

ctvolume

CVOLUMEClears both the infused and withdrawn volumes. QuickStart mode only.

Command format:

cvolume

CWVOLUMEClears the withdrawn volume. Quick Start mode only.

Command format:

cwvolume

IVOLUMEDisplays the infused volume. Quick Start mode only.

Command format:

ivolume

Query response:

<lf>[##:]# xl<cr>

<lf>[##]<prompt>

SVOLUMESets or displays syringe volume. Quick Start mode only.

Command format:

svolume

TVOLUMESets or displays the target volume. Quick Start modeonly.

Command format:

tvolume [target volume volume units]

Query response:

<lf>[##:]Target volume not set<cr>

<lf>[##]<prompt>

or:

<lf>[##:] # xl/xxx<cr>

<lf>[##]<prompt>

WVOLUMEDisplays the withdrawn volume. Quick Start mode only.

Command format:

wvolume

Query response:

<lf>[##:]# xl<cr>

<lf>[##]<prompt>

For commands requiring rate units, use thefollowing format: m, u, n, p/h, m, s. ex: m/m =milliliter/minutes ex: n/s = nanoliter/second


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Time commands

CITIMEClears the infused time. Quick Start mode only.

Command format:

citime

CTIMEClears both the infused and withdrawn times. QuickStart mode only.

Command format:

ctime

CTTIMEClears the target time. Quick Start mode only.

Command format:

cttime

CWTIMEClears the withdrawn time. Quick Start mode only.

Command format:

cwtime

ITIMEDisplays the infused time. Quick Start mode only.

Command format:

itime

Query response:

<lf>[##:]# seconds<cr>

<lf>[##]<prompt>

or:

<lf>[##:]##:##:##<cr>

<lf>[##]<prompt>

TTIMESets or displays the target time. Quick Start mode only.

Command format:

ttime [target time]

Query response:

<lf>[##:]Target time not set<cr>

<lf>[##]<prompt>

or:


<lf>[##]<prompt>

or:

<lf>[##:]##:##:##<cr>

<lf>[##]<prompt>

WTIMEDisplays the withdrawn time. Quick Start mode only.

Command format:

wtime

Query response:


<lf>[##]<prompt>

or:

<lf>[##:]##:##:##<cr>

<lf>[##]<prompt>

Digital I/O commands

INPUTReads and displays the trigger input port status.

Command format:

input

Query response:

<lf>[##:]The trigger input is low.<cr>

<lf>[##]<prompt>

or:

<lf>[##:]The trigger input is high.<cr>

<lf>[##]<prompt>


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OUTPUTSets the level on the output port.

Command format:

output 1 high | low

Internal commands

STATUSDisplays the raw status for use with a controllingcomputer.

Command format:

status

Serial Command NotesThe output is in three integer fields and one flag field, allseparated by spaces and terminated by a carriagereturn/linefeed pair. The first integer is the current ratein femtoliters per second. The second integer is theinfuse time in clock cycles. One clock cycle is1/56,000,000 second. The time has a granularity of 1millisecond. The third integer is the infused volume infemtoliters. All three values are for the current direction.

The flag field consists of six flags.

Flag one is the motor direction and will be “I” if thepump is infusing and “w” if the pump is withdrawing. Ifthe letter is lower case, the pump motor is idle. If uppercase, the pump motor is running.

Flag two is the limit switch status. If the infuse limitswitch was hit, “I” is displayed. If the withdraw limitswitch was hit, “W” is displayed. If no limit switch washit or the pump does not have limit switches, “.” isdisplayed.

Flag three is the stall status and will be “S” if the pumphas stalled. Otherwise it will be “.”.

Flag four is the trigger input state and will be “T” if highand “.” if low.

Flag five is the direction port state and will be “I” forinfuse and “W” for withdraw.

Flag six is the target reached status. It will be “T” if thetarget time or volume was reached and “.” if not.

The Pump 11 Elite does not contain limitswitches, so “.” will be displayed.

58Appendices

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Appendices

APPENDIX A: SYRINGE VOLUME/DIAMETER REFERENCE TABLE

Harvard ApparatusStainless Steel

Size Diameter

2.5 ml 4.851 mm8 9.525

20 19.1350 28.6

Air–TiteHSW Norm-JectSize Diameter

1. ml 4.69 mm2.5 9.655 12.45

10 15.920 20.0530 22.950 29.2

Becton Dickinson‘Plasti-pak’

Size Diameter

1. ml 4.699 mm3 8.5855 11.989

10 14.42720 19.0530 21.5950 26.59460 26.594

TerumoSize Diameter

1. ml 4.70 mm3 8.95 mm5 13

10 15.820 20.1530 23.160 29.1

SGE ScientificGlass

EngineeringSize Diameter

5. µl 0.343 mm10 0.48525 0.72850 1.03

100 1.457250 2.303500 3.257

1. ml 4.606 mm2.5 7.2845 10.301

10 14.56725 2350 27.5

HamiltonGlass - All TypesSize Diameter

0.5 µl 0.103 mm1 0.1462 0.2065 0.343

10 0.48525 0.72950 1.03

100 1.457250 2.304500 3.256

1. ml 4.608 mm1.25 5.1512.5 7.2855 10.3

10 14.56725 23.03350 32.573

Cadence Science,Inc.

Formerly Popper &Sons MICRO-MATE

Glass

Size Diameter

0.25 ml 3.47 mm0.5 3.621 4.822 8.913 8.915 11.71

10 14.6520 19.5630 22.750 28.02

Becton DickinsonGlass-All Types

Size Diameter

0.5 ml 4.64 mm1 4.642.5 8.665 11.86

10 14.3420 19.1330 22.750 28.6

Sherwood–Mono-

jectPlastic

Size Diameter

1. ml 4.674 mm3 8.8656 12.600

12 15.62120 20.14235 23.57160 26.568

HoshiSize Diameter

1. ml 6.50 mm2 9.103 10.005 12.60

10 15.1020 20.4530 22.5050 25.60

NatsumeSize Diameter

0.25 ml 2.5 mm0.50 2.81 4.02 6.23 7.45 9.2

TopSize Diameter

1. ml 6.40 mm2.5 9.305 13.10

10 15.320 21.030 23.050 29.0

NIPROSize Diameter

1. ml short 6.6 mm1. ml long 4.72.5 ml 9.05 13.0

10 15.820 20.130 23.250 29.1

Suggested Force Level Settings for Common SyringesSyringe Type/Material Capacity Force Setting Common Manufacturer(s)Plastic Syringes ≤5ml 50% BD plastic, Sherwood, Airtite, Terumo

>5ml 100%

Stainless Steel All 100% Harvard Apparatus

Glass/Glass ≤20ml 30% Cadence (Popper)

>20ml 50%

Glass/Plastic ≤250µl 30% Hamilton, SGE

≤5ml 50%

>5ml 100%

The Pump 11 Elite can accommodatesyringes ranging from 0.5 µl to 60 ml singlesyringe and 0.5 µl to 10 ml dual syringe.

NOTE: The Pico Plus Elite can accommodatesyringes ranging from 0.5 µl to 10 ml.

59Appendices

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Nominal Minimum/Maximum Flow Rates for Various Syringes.(Actual Limits will vary depending on syringe manufacturer)

Syringe Size Syringe ID MinimumRate Units Maximum

Rate Units

0.5 µl 0.103 mm 1.26 pl/min 1.325 µl/min

1 µl 0.146 mm 2.52 pl/min 2.65 µl/min

2 µl 0.206 mm 5.1 pl/min 5.299 µl/min

5 µl 0.343 mm 14.1 pl/min 14.69 µl/min

10 µl 0.485 mm 28.26 pl/min 29.38 µl/min

25 µl 0.729 mm 63.9 pl/min 66.37 µl/min

50 µl 1.030 mm 127.6 pl/min 132.5 µl/min

100 µl 1.457 mm 255.2 pl/min 265.1 µl/min

250 µl 2.304 mm 638.3 nl/min 662.9 µl/min

500 µl 3.256 mm 1.275 nl/min 1.324 ml/min

1000 µl 4.608 mm 2.553 nl/min 2.652 ml/min

1 ml 5.151 mm 3.191 nl/min 3.313 ml/min

3 ml 8.585 mm 8.863 nl/min 9.204 ml/min

5 ml 11.99 mm 17.29 nl/min 17.95 ml/min

10 ml 14.43 mm 25.03 nl/min 25.99 ml/min

20 ml 19.05 mm 43.64 nl/min 45.32 ml/min

30 ml 21.59 mm 56.05 nl/min 58.21 ml/min

50 ml 26.59 mm 85.05 nl/min 88.32 ml/min

60 ml 26.59 mm 85.05 nl/min 88.32 ml/min

APPENDIX B: PUMP 11 ELITE MIN/MAX FLOW RATES

60Appendices

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Nominal Minimum/Maximum Flow Rates for Various Syringes.(Actual Limits will vary depending on syringe manufacturer)

Syringe Size Syringe ID MinimumRate Units Maximum

Rate Units

0.5 µl 0.103 mm 0.54 pl/min 596.2 nl/min

1 µl 0.1457 mm 1.14 pl/min 1.193 µl/min

2 µl 0.206 mm 2.28 pl/min 2.385 µl/min

5 µl 0.343 mm 6.36 pl/min 6.612 µl/min

10 µl 0.485 mm 12.72 pl/min 13.22 µl/min

25 µl 0.729 mm 28.74 pl/min 29.87 µl/min

50 µl 1.030 mm 57.36 pl/min 59.62 µl/min

100 µl 1.457 mm 114.8 pl/min 119.3 µl/min

250 µl 2.304 mm 287.2 nl/min 298.3 µl/min

500 µl 3.256 mm 573.7 nl/min 595.8 µl/min

1000 µl 4.608 mm 1.149 nl/min 1.193 ml/min

1 ml 4.699 mm 1.195 nl/min 1.241 ml/min

3 ml 8.585 mm 3.988 nl/min 4.142 ml/min

5 ml 11.989 mm 7.778 nl/min 8.078 ml/min

10 ml 14.430 mm 11.26 nl/min 11.7 ml/min

PICO PLUS ELITE MIN/MAX FLOW RATES

61Appendices

APPENDIX C: EXTERNAL CONNECTIONS

User I/O Connector Specifications

Pin Assignments

1. Direction control input

Rising edge sets pump to infuse

Falling edge sets pump to refill

2. Trigger Input

Event trigger – falling edge triggers a Methodevent

3. Footswitch Input

Momentary switch closure to ground or TTLLogic Low; falling edge toggles between states(run/stop)

4. Trigger 1 Output

TTL Logic Output-Default = Low Control thruMethod or serial comm

9-13. Signal Return / Ground

14. Run Indicator Voltage

+5V through a 470 ohm resistor(connect to LED Anode)

15. Run Indicator Output

TTL Logic Output, active low(low = run) (connect to LED Cathode)

Electrical Specifications

Inputs: VIH ≥ 2V IIH ≤ 20µAVIL ≤ 0.4V IIL ≤ 0.5mA

Outputs: VOH ≥ 3.8V IOH ≤ 6mAVOL ≤ 0.4V IOL ≤ 6mA

All Inputs are pulled high to +5 Vdc through a 10kresistor All Outputs are at TTL Logic Levels

User I/O Connector Specifications

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62Appendices

Utilizing the USB Interface

12

3 4

Pin # Signal

1 +5V

2 - Data

3 + Data

4 GND

Mating Face

USB VIRTUAL COMMPORT DRIVER INSTALLATION

When you connect the Pump 11 Elite Series pump to acomputer via USB for the first time, Windows will seekto install a driver for communication. The followingsection details the installation of the Virtual CommPortDriver supplied with the Pump 11 Elite Series Pump.

1. On connection of the pump to the computervia USB, the Found New Hardware Wizard willlaunch and ask how you wish to locate thedriver. Choose “No, not this time” and clickNext.

2. Choose “Install from a list or specific location(Advanced)” and click Next.

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63Appendices

3. Browse for the proper USB driver installationfolder in the USB folder and then click next. Itcan be found on the CD supplied with thePump 11 Elite Series Pump.

USB Driver: WindowsXP or Vista

USB Driver: Windows2000

4. If a warning message regarding WindowsLogo testing is displayed, choose ContinueAnyway to proceed with the installation.

5. The Virtual Commport software will beinstalled. When the software installation iscomplete, click Finish to close the wizard.

USB VIRTUAL COMMPORT DRIVER INSTALLATION (CONTINUED)

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64Appendices

RS-485

1 2

3 4

5 6

Pin # RS-485 In Signal RS-485 Out Signal Notes

1 PWR IN PWR OUT (+30V)*

2 GND GND

3 RS-485 A TIED TO PIN 4**

4 RS-485 B TIED TO PIN 3**

5 TIED TO PIN 6** RS-485 A

6 TIED TO PIN 5** RS-485 B

Mating Face

* Protected byresettable fuse

**100 Ω, ½ w toGND termination

RS-485 In/Out - IEEE-1394 Sockets

Daisy-Chaining via RS-485

P.C. PUMPADDR Ø

USBUSB

RS-485OUT

PUMPADDR 1

RS-485OUT

RS-485IN

PUMPADDR n

RS-485IN

RS-485

IEEE 1394Cable

RS-485• • •

IEEE 1394Cable

! CAUTION: DO NOT CONNECT TO FIREWIRE PORTS ON A PC.DAMAGE MAY OCCUR TO PUMP AND/OR PC.

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MASTER PUMPADDR Ø

RS-485OUT

PUMPADDR X

RS-485OUT

RS-485IN

RS-485

IEEE 1394Cable

Gradient Connections via RS-485

When daisy-chaining with RS-485, atminimum, every fourth pump must be selfpowered (i.e., another stand-alone unit).This can be affected by Force Selection.

When applying power to pumps in a daisy-chain, apply power to the last group andwork backwards.

When removing power, be sure to rememberthat power removed from a stand-alone unitwill also cause power to be removed fromall RS-485 powered units deriving powerfrom it.

Power may be removed from any group ofpumps, except the first group, withoutaffecting communications “down-stream’.

65Appendices

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Daisy-Chaining with Legacy RJ-11 Option

Pump

Transmit

Port #2(out)

Receive

Ground

Ground

Transmit

Receive

Port #1(in)

Legacy RJ-11 Option

Pumps with Legacy RJ-11 Option

P.C. PUMPADDR Ø

RJ-11IN

RJ-11OUT

PUMPADDR 1

RJ-11OUT

RJ-11IN

PUMPADDR n

RJ-11IN

RS-232

D-Sub 9 F toTelephone

Cable Cat #70-2022

RS-232

Non-StandardTelephoneCableCat #

55-7760or 72-2478

RS-232• • •

Non-StandardTelephone

Cable Cat #55-7760

or 72-2478

Pumps 1...n also have RJ-11 telephone jacks.

66Appendices

APPENDIX D: MAINTENANCE

MaintenancePump 11 Elite Series pumps require no specialmaintenance other than keeping them clean by avoidingaccidental spills of pumped material. Refer to thechemical compatibility chart below for specific chemicalresistance information.

The two guide rods and the lead screw should besparingly lubricated periodically; every 100 hrs the pumpwill remind you. The guide rods and the lead screw

should be lubricated with Super Lube Synthetic Greaseprovided with the pump.

To clean the exterior surfaces, use a lint-free cloth toremove loose dust. Use care to avoid scratching theclear display window. For more efficient cleaning, use asoft cloth dampened [not soaked] with water, anaqueous solution of 75% isopropyl alcohol, or a milddetergent.

A SatisfatoryB Fair, Minor EffectsC Poor, Moderate EffectsD Severe Effects- Not tested

The chemical resistance data provided is forreference only. The data is based on themanufacturer’s recommendations and is not aguarantee of chemical compatibility for your testconditions. Wipe any spills immediately.

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Acetonitrile 99.5% A AMethyl alcohol 99.8% A -

Hydrochloric Acid 36.5% A ABleach 100% A A

Windex® 100% A AIsopropyl Alcohol 99.5% A D

Dish Soap - A AMotor Oil 100% A A

Simple Green 100% A AGasoline 100% A D

Ethylene Glycol(Antifreeze) 100% A A

Mineral Oil 100% A AAmmonia 27.0% A A

Magnalube 100% A ASuperlube 100% A AAcetone 99.5% A D

Soda (Coca-Cola)® 100% A ASulfuric Acid 95.0% D D

Dimethyl Sulfoxide 99.9% A CSodium Hydroxide 50% A A

Nitric Acid 68.0% D DChloroform 100% D D

Chemical Compatibility Chart

Chemical Concentration Overlay Cover Overlay Adhesive

67Appendices

Upgrading Pump 11 Elite SeriesSoftware

1. Upload the latest software version to yourdesktop (format is filename.dfu).

2. Connect the pump to PC using a USB cable.

3. On the Settings screen, press the UpgradeSoftware button twice to enter “Boot LoaderMode”. The pumps screen will be blank andthe light should be flashing.

4. Disconnect then reconnect the USB cable.

5. From the CD provided with the pump, open thePump 11 Elite Series Flash UpgradeApplication.

6. Check “Verify after download” then clickbrowse. Browse to the file previously saved onyour desktop and click open.

7. Click upgrade. The previous software will firstbe erased, the new software will be loaded,and finally the software will be verified.

8. Disconnect the USB cable and power cyclethe pump.

To exit boot loader modewithout upgradingthe software, power cycle the pump.

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First time users will need to install thePump 11 Elite Series USB driver as well asthe bootloader driver file (Pump 11 EliteSeries-Bootloader.inf). Reference “USBVirtual Compact Driver Installation”.

68Appendices

TROUBLESHOOTING

1. Infusion AccuracyTo ensure infusion accuracy always use new syringe(s)and measure syringe bore diameter and enter actualdimensions in millimeters (mm) using the CustomSyringe entry option. Additionally, make sure that theguide rods and lead screw are properly lubricated.

Pump 11 Elite Series DisplayError Messages

2. Motor StalledThis indicates that the pusher block travel has beenimpeded. This may be caused by the syringe plungerhitting bottom, a kink in the tubing (occlusion), syringeplunger binding, incorrect positioning of mechanical stopcollars or any situation requiring more force to the headof the syringe than the pump is capable of delivering. Torestart the pump, remove the cause of stalling and/orincrease the force setting, then press RUN. The pumpwill continue the interrupted procedure where itstopped.

3. Out of RangeA value was entered or encountered in a pump Methodthat was beyond the pump’s limits.

4. Power Failure NotificationIf power to the pump is interrupted during operation, analarm will sound (if alarm is activated) to alert the userto the interruption. When power is restored, anonscreen message is displayed to indicate theinterruption.

5. Overload ProtectionThe Pump 11 Elite incorporates resettable fuses foroverload protection. In the event of an overload, powerto the pump may be disconnected. Try to determine andremove the source of the overload, wait a few minutes,and then re-apply power to the pump.

The Pump 11 Elite Series Pump will notify you of a power failurewith a message when power is restored

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It is recommended that you save yourMethods periodically by uploading them toa PC.

69Appendices

APPENDIX E: ORDERING INFORMATION

Standard VersionDescription Single Syringe Dual Syringe

Pump 11 Elite Infusion Only 70-4500 70-4501

Pump 11 Elite Infusion/Withdrawal Programmable 70-4504 70-4505

Pump 11 Pico Plus Elite Infusion/Withdrawal Programmable — 70-4506

OptionsDescription Catalog Number

RS-232 RJ-11 Connectors Option 70-3030(Only available with Programmable Models)

*If needed, must be ordered at the same time as the Elite pump

AccessoriesDescription Catalog Number

RS-485 Cable for Pump-to-Pump Communication, 0.5 m (2 ft) 70-4000

RS-485 Cable for Pump-to-Pump Communication, 1 m (3 ft) 70-4021



USB Cable for PC-to-Pump Communication, 2 m (7 ft) 70-4002

USB Cable for PC-to-Pump Communication, 5 m (16 ft) 70-4003

Cable Assy, Daisy-chain, Legacy RS-232 RJ-11, 0.5 m (2 ft) 55-7760

Cable Assy, Daisy-chain, Legacy RS-232 RJ-11, 2 m (7 ft) 72-2478

Line Cord, US (115 v) 5113-001

Adapter, Digital I/O 70-4005

Adapter, D-sub 15 to Term. Blk 70-4006

Footswitch (w/ Phono Plug) 70-2215

Adapter for 25ml, 50ml Hamilton GasTight™ syringes 55-8000

Hex Key, 3/32 5012-005

Lubricant, SuperLube, 1cc 70-4013

For additional items such as Syringes, Tubing, Syringe Heaters, Spill Sensors, Temperature Controllers, Nano-FluidicAccessories, and much more please visit our website www.harvardapparatus.com or call (800) 272-2775.

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Declaration of ConformityManufacturer: Harvard Apparatus, Inc.

84 October Hill RoadHolliston, Massachusetts 01746-1388, U.S.A.Phone: (508) 893-8999

We herewith declare that the following product:

Product Name: Pump 11 Elite Syringe Pump SeriesModel No.: Pump 11 Elite - Catalog # 70-45xx

To which this declaration relates, is in conformity with the applicable EC Directives, harmonizedstandards, and other normative requirements:

Application ofCouncil Directive(s): 2006/95/EC Low Voltage Directive

2004/108/EC Electromagnetic Compatibility Directive2002/95/EC RoHS Directive

Standard(s) to whichconformity is declared:

Safety: EN 61010-1:2001 (2nd Edition)Emissions/Immunity: EN 61326:2006/05/01

EN 61000-4-2:1995 + A1:1998+A2:2001EN 61000-4-3:2002EN 61000-4-4:2004EN 61000-4-5:1995+A1:2001EN 61000-4-6:2007EN 61000-4-8:1993+A1:2001EN 61000-4-11:2004EN 61000-3-2:2000EN 61000-3-3:1995+A1:2001CISPR11:2003+A1:2004, Group 1, Class BFCC Part 15 Class B

EMC and Safety compliance were evaluated by Intertek/ETL Semko

Reference test reportfile numbers: 100071222 BOX-002, -004a, -007

I, the undersigned, hereby declare that the equipment specified above conforms to the above Directive(s) and Standard(s).

Place: United States of AmericaDate: April 22, 2010

Beth Bauman

VP Engineering / Operations

(Signature)

(Full Name)

(Position)

Documents

Pump 11 Elite & Pump 11 Pico Plus Elite cfile.yizimg.com/528182/20171129-172413520.pdfHarvard Apparatus Pump 11 Elite & Pump 11 Pico Plus Elite Series Publication 5420-002 Rev B w