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Purple Non Sulfur Bacteria Hopkins Microbiology Course Ann Lesnefsky 7-15-2011

Purple Non Sulfur Bacteria

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Purple Non Sulfur Bacteria. Hopkins Microbiology Course Ann Lesnefsky 7-15-2011. Metabolism of PNS Bacteria. Organic acid electron donor Photosynthesis for energy generation Bacterial chlorophyll b Absorbs light in the infrared range - PowerPoint PPT Presentation

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Page 1: Purple Non Sulfur Bacteria

Purple Non Sulfur Bacteria

Hopkins Microbiology CourseAnn Lesnefsky 7-15-2011

Page 2: Purple Non Sulfur Bacteria

Metabolism of PNS Bacteria

• Organic acid electron donor• Photosynthesis for energy

generation– Bacterial chlorophyll b– Absorbs light in the infrared

range• Currently PNS bacteria are

being studied for H2 production

Page 3: Purple Non Sulfur Bacteria

Course Enrichment Method for PNS

• 6-18-2011– Plated samples from the slough on succinate and

ethanol plates, incubated anaerobically with light– Course’s media and Holly’s media

• 6-29-2011– Restreak single colonies for isolation

Page 4: Purple Non Sulfur Bacteria

TA Enrichment Method for PNS

• TA’s enriched for PNS by filtering– Filter both water above mat and

slurry of mat material– Place filter face down on plate– Flip and all allow PNS bacteria to

grow on filter

Page 5: Purple Non Sulfur Bacteria

Carbon Source

Enrichment Method

Succinate (# of colonies before restreak)

Ethanol (# of colonies before restreak)

No filter Course

43 6

Holly

0 0

Filter Course

Many

Some

Holly

Few None

Page 6: Purple Non Sulfur Bacteria

Microscopy of PNS• Colonies isolated from 5 different plates– 4 plates from strawberry– 1 from Kerby Park

• All looked like stubby rods

Page 7: Purple Non Sulfur Bacteria

Recommendations

• Next year use the filter method.

Page 8: Purple Non Sulfur Bacteria

Questions?