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Putative function # A.As Forward primer (5’→3’) Reverse primer (5’→3’)1 ABR76064 2,3-dihydroxybenzoate-2,3-dehydrogenase 756 TCTAGAatggcggcgctggattt ACTAGTCTCGAGttacgcccccagcgtcga2 ABR76070 putative short-chain alcohol dehydrogenase 744 TCTAGAatgcagatcgatttaacaggta GGATCCCTCGAGtcaggcgttcaatccgcc3 ABR76174 succinate dehydrogenase catalytic subunit 717 TCTAGAatgaaactcgagttttcaatttatc GGATCCGTCGACttacgcgctgcgctgca4 ABR76263 putative short-chain dehydrogenase/reductase SDR 750 TCTAGAatgttattgaaagataaagtcgcc GGATCCCTCGAGttagtgaattaacatgccgc5 ABR76673 putative 2-deoxy-D-gluconate 3-dehydrogenase 762 TCTAGAatggtactgaacgccttcga ACTAGTtcagcgcgccagccatc6 ABR76751 7-alpha-hydroxysteroid dehydrogenase 735 TCTAGAgtgacaggtggcagccg GGATCCCTCGAGtcagatgcgcattcccc7 ABR76954 short chain dehydrogenase 723 TCTAGAatggctgaacaacagccc GGATCCCTCGAGctagcgcagcgggcgg8 ABR77125 putataive short-chain dehydrogenase/reductase 735 TCTAGAatgaaacttgccagtaaaaccg GGATCCCTCGAGttacagcaccatgccgcc9 ABR77213 putative deoxygluconate dehydrogenase 750 TCTAGAatgaagatgctgagaggtaaaag GGATCCCTCGAGtcagcttctgactgccagtt
10 ABR77300 putative 1,2-dihydroxycyclohexa-3,5-diene carboxylate dehydrogenase 774 TCTAGAatgcgttttaccgataaagtcgt GGATCCCTCGAGttagccgagatcgcctccg11 ABR77436 short chain dehydrogenase 741 TCTAGAatgatcaccggctgttcatc GGATCCGTCGACtcagacaaggcgatgactgg12 ABR77485 short chain dehydrogenase 771 TCTAGAatgaacttacacggtaaaacgg GGATCCCTCGAGttactgcgccacccagc13 ABR77489 short chain dehydrogenase (Ard) 771 TCTAGAatgaaaaaagtcgcacttgttac GGATCCGTCGACttagttaaacaccatcccgcc14 ABR78386 putative short-chain dehydrogenase/reductase 795 TCTAGAatgaaaattgatttagcgggaaaa GGATCCCTCGAGtcagagaatatcatccaccacg15 ABR78441 sorbitol-6-phosphate 2-dehydrogenase 780 TCTAGAatgaatcaggttgctgttgtca GGATCCCTCGAGttaaaacatcacctggccgc16 ABR78663 2-deoxy-D-gluconate 3-dehydrogenase 762 TCTAGAatgattcttgatgcattctccc ACTAGTCTCGAGttagcgcgccagccag17 ABR78759 putative aspartate dehydrogenase 768 TCTAGAatgatgaaaaaggtcatgttaattg GGATCCCTCGAGttatgccagttcacggcagg18 ABR78760 putative short-chain dehydrogenase 795 TCTAGAatgaatgcacaaattgaaggg ACTAGTCTCGAGttacagatggcgacagaaacc19 ABR78766 putative short-chain dehydrogenase 750 TCTAGAatgaacggcctgctaaacgg ACTAGTCTCGAGttagttaaagacaaaaccgccg20 ABR80103 putativae NAD(P)H dehydrogenase (quinone 783 TCTAGAatgaaagtactgctgatttacgcc GGATCCCTCGAGttattcgctacgcaaatgca
Journal of Industrial Microbiology and Biotechnology
Identification and characterization of a short-chain acyl dehydrogenase from Klebsiella pneumoniae and its application for high level production of L-2,3-butanediol Jang Min Park Won-Kyung Hong Sung-Mok Lee Sun-Yeon Heo You Ree Jung In Yeong Kang Baek-Rock Oh Jeong-Woo Seo () Chul Ho Kim ()Biorefinery Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk 580-185, South KoreaTel: +82 63 570 5160; Fax: +82 63 570 5109; E-mail: [email protected] (J. W. Seo); Tel: +82 63 570 5110; Fax: +82 63 570 5109; E-mail: [email protected] (C. H. Kim)
Table. S1. Oligomers used to express deydrogenase gene of K. pneumoniae
Restriction enzyme sites: bold capital letter, XbaI; bold italic letters, SpeI; italic letters, XhoI; lined italic letters, BamHI; lined letters, SalI.
