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RainbowElectrophoresis
TECHNIQUE USED TO IDENTIFY INDIVIDUALS BY THEIR DNA PROFILES
DNA Fingerprinting
The Importance of DNA Fingerprinting
PaternityTests
MedicalDiagnosis
Crime SceneInvestigation& Forensics
ForensicAnthropology
Learning Objectives
At the end of this lesson, you will be able to…
(1)Come up with ideas of how DNA fingerprinting can be applied in different areas
(2)Perform a gel electrophoresis experiment
(3)Define PCR and DNA gel electrophoresis
(4)Predict the results of the gel electrophoresis experiment according to different hypotheses
(5)Determine the answer to a case study problem by analyzing gel electrophoresis results
What are…DNA
Genes
Sequence
Polymorphic DNA Regions
Some regions of the human chromosome are polymorphic or dimorphic
e.g. Dimorphic TPA (Tissue Plasminogen Activator) gene: Some TPA alleles possess Alu elements.
Polymerase Chain Reaction
PCR (Polymerase Chain Reaction): an enzymatic amplification process
Videohttp://www.sumanasinc.com/webcontent/
animations/content/pcr.html
Gel Electrophoresis
Gel Electrophoresis:process in which molecules (e.g. DNA) are separated according to size and electrical charge by applying an electric current to the gel matrix
Videohttp://www.sumanasinc.com/webcontent/
animations/content/gelelectrophoresis.html
RainbowElectrophoresis
Questions?
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