Forensics Magazine Webinar: May 8, 2014

Presenters:

Rapidly Detect,

Identify, and Monitor

Unknown Substances

- Anywhere

Alan Rein Thomas Szakas Agilent Technologies Agilent Technologies

For Forensic Use

At-Site Analysis Supports Forensic Investigations

•Aid in establishing probable cause, allowing seizure

of material for further analysis

•Reduce the audit trail and shorten the chain of evidence

•Enable real-time decisions about what to analyze and where to sample

•Identify the nature of a threat and establish perimeters for responders and civilians.

•Assess the progress of on-going decontamination operations and confirm

remediation of the incident site.

•Aid personnel involved in border control, corporate safety, mail room and post

office security

•Support the identification and seizure of counterfeit pharmaceuticals, illicit drugs

and related chemicals

•Analyze toxic industrial chemicals, chemical agents, white powders, and explosives

For Forensic Use

Agilent Mobile GC-MS and FTIR Analyzers – Powerful tools for at-site Forensic Analysis

•Rapid, reliable analysis of unknown compounds and materials is critical in forensic,

environmental, homeland security and military applications.

•Agilent provides key technologies that are optimized for at-site analysis and enable

sampling of solids, liquids, air, gels, foods, and even blood.

•Agilent's mobile GC/MS, and FTIR analytical instruments, enable lab quality results

in the field.

In the homeland security and military fields rapid detection and identification

of chemical warfare agents (CWAs) and toxic industrial chemicals (TICs) is

essential.

In forensic and investigative fields, the speed and accuracy of results are

critical. Test for poisons in a forensics investigation, analyze explosives and

their residues, check a crime scene for illicit drugs, precursor chemicals and

cutting agents.

For Forensic Use

Agilent At Site Analyzers for Forensic Analysis

5975T LTM GC/MSD 4500 Portable FTIR

4300 Handheld FTIR

For Forensic Use

June 11, 2014

Agilent 5975T GC/MSD & 4500/4300 FTIR

Agilent At Site Forensic Analyzers – Cost effective and

Well Suited to At-Scene and Field Lab Use

For Forensic Use

IR Spectroscopy Provides

Each structural characteristic of a molecule has a unique vibrational frequency that can

identify the functional group present. When all the different functional groups in a molecule are

simultaneously measured, a unique molecular "fingerprint" provides the chemical identity of a

sample.

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3950 3900 3850 3800 3750 3700 3650 3600 3550 3500 3450 3400 3350 3300 3250 3200 3150 3100 3050

0.22 0.20 0.18 0.16 0.14 0.12 0.10 0.08 0.06 0.04 0.02 0.00 -0.02 -0.04 -0.06 -0.08

Wavenumber

Ab

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Spectral Identification

of Unknowns

Quantitative analysis of

sample components

For Forensic Use

FTIR Analyzers for At-Site Forensic Analysis

4500a Portable FTIR

• 15 lbs (6.8 kg)

• detachable PDA computer

• Internal battery

• ATR sample interface

• For field use in non-hot zones

• Cost effective

For Forensic Use

4500a Series FTIR Sample Interface

ATR • Simple, easy to use

- <2mg sample placed on sensor,

- pressure device ensures contact,

- answer provided in <30 secs.

• Short path length ~2 µm- Library match, product identification

• Uses Diamond sensor- Chemical and scratch resistant

- Internal reflection

For Forensic Use

Guided User Interface

Leads analyst

through the

sampling process

at the scene.

For Forensic Use

FTIR Forensic Analyzer Software

• Method Driven

• Focused on Results

• Saves original data

• Synchronizes with PC

software

• Completely functional

software for Point of

Measurement Applications

• Quantitation

• Qualitative Library Search /

Identification

13,000 spectra from a comprehensive list of:

Bio-chemicals

Forensics

White powders

Hazardous and Toxic Chemicals

HPV chemicals

Food additives

Explosives

For Forensic Use

Identification of IIlicit Drugs

Agilent 4500a PDA computer controls all operations of the system.

After spectra of an unknown is acquired, a pre-loaded method

automatically searches an on-board forensic substance library and

displays both the identity of the unknown, the quality of the match as

well as the spectrum of the material

For Forensic Use

Identification of IIlicit Drugs

• Spectra of street drugs searched

against the Agilent Forensic Library.

• Search reveals:

spectrum of the unknown

sample

spectrum of best match

sample identity

numerical quality of the

match.

For Forensic Use

Identifying Components in Drug Mixture

• Spectral search result of a sample

suspected of containing cocaine.

The spectral search indicates a

mixture of cocaine,

acetophenetidine and p-

acetophenetidine. The quality

index is low due to the mixture of

the two main components.

• With residual search, the first

match is subtracted and the

residual spectrum is automatically

searched against the library.

• The residual spectrum matches

well with cocaine based (crack)

and the match quality is quite

good.

Search After Spectral

Subtraction of Best Match

from Drug Sample

Red – Spectrum of Drug Sample

Blue - Spectrum of Best Match

For Forensic Use

FTIR Analyzers for At-Site Forensic Analysis

4300 Handheld FTIR

• 4.8 lbs (2.2 kg)

• Integrated computer

• “Hot swap” internal

batteries

• Selectable sample

interfaces

• For field use in non-hot

zones

ATR - for solids, liquids, pastes and gels. Diffuse Reflectance - for scattering samples

For Forensic Use

Spectra of Motorcycle and Truck Components

Pillion case

Truck Paint

Pillion Case with

residual Truck Paint

For Forensic Use

How do you take sample characterization to the next level?

FTIR + GCMS

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For Forensic Use

Gas Chromatography - Mass Spectrometry GCMS

1. What is GC/MS and what does it tell us?

2. Advances in technology to ‘bring it to the field’

3. Threat Detection by flexible sampling techniques

4. Relevent data

5975T GC/MSD

Mobile, Portable,

Transportable….

For Forensic Use

5975T：Mobile Lab Process Savings

Ship sample Sample

Prep Test

Data Processing

Fixed Lab

Sample Prep

Test Data

Process

Mobile Lab

For Forensic Use

5975T:1st Agilent Lab Performance Transportable GC/MS

5975C LTM MSD

ChemStation

For Forensic Use

A Fast and Simple alternative probe for analyzing solid, liquid, slurry and

vapor samples in the field.

TSP: Fast and Simple Sample Prep

For Forensic Use

1. Probe

2. TSP Adapter

3. Inlet liner

4. TSP sample

vial

How does the TSP work?

Steps:

1.Place a small amount of liquid or solid in

the sample vial

2.Load vial into probe

3.Insert probe into inlet via TSP adapter

4.Re move probe and discard vial

For Forensic Use

Capillary-Trap Sampler(CTS) for Vapor Sampling

A fast and simple to use gas sampler for trace level target

compounds (ppb~ppm) sampling in field.

Gas

pump Trap

head

Trap

columns

For Forensic Use

How does the CTS Works?

1. Install trap column into CTS head

2. Pump air through trap columns 0.5~2Min to trap target compounds

• Up to 6 unique samples may be collected with one sampler

3. Remove the columns to TSP vial

4. Run analysis by TSP

For Forensic Use

Ultra-fast fire accelerants analysis of fire debris with 5975T+CTS+TSP

• Ultra fast in sampling with CTS: 1min

• Ultra fast in 5975T+TSP analysis: 5min

• Ultra fast in data handling: 1min

For Forensic Use

TIC Contrast: Save Time for Gasoline Confirmation

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5975T GCMS:

5min

Typical Lab

GCMS:40min

For Forensic Use

Page 26

Gasoline sample Running time :

5min

For Forensic Use

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Banana oil Running time :

5min

For Forensic Use

Page 28

Running time :

5.5min

Banana oil+ gasoline

For Forensic Use

Extended Applications with 5975T Method

Page 29

Aviation kerosene

Diesel

For Forensic Use

Rapid Pesticide Residue Identification with 5975T + TSP

Simplified QuEChERS method w/o Clean-up

TSP: the RSD% of all the compounds is from 2.3 ~7.8% with 1ul injection

Running time <5 Min

For Forensic Use

RT Compounds

1.8765 Barbital

2.3049 Amobarbital

2.4384 Secobarbital

3.1265 Cocaine

3.2050 1-Piperidinepropanol, .alpha.-cyclopentyl-.alpha.-phenyl-

3.2407 Promethazine

3.2788 SKF525

3.3232 Oxazepam

3.4365 Lorazepam

TIC of Drugs

Standard

3.4782 Diazepam

3.563 Chlorpromazine

3.5645 Chlorprothixene

3.5950 Chlordiazepoxide

3.9862 Papaverine

4.024 Clozapine

4.0243 Clonazepam

4.0971 Estazolam

Rapid Drug Identification by 5975T+TSP

For Forensic Use

Rapid Explosives Identification by 5975T + TSP

Soil matrix,SQCI-011;

1,2,3:2-Nitrotoluene,3-nitrotoluene,4-

nitrotoluene; 4: PETN; 5:1,3-Dinitrobenzene;

6: 2,4-dinitrotoluene; 7: 2,6-dinitrobenzene;

8,9,10: TNT mixture; 11: RDX; 12 3,4,5-TNT;

13: 2-Amino-4,6-dinitrotoluene;

14 :4-amino-2,6-dinitrotoluene

1

2

3

4

5 67

8

9 10

11

12

13 14

Running time< 5Min

No sample preparation needed, just measure sample powder

Fast

Temperature

Program

method

For Forensic Use

So, how does all this technology fit together?

June 11, 2014

Confidentiality Label

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For Forensic Use

Real World Example – Phthalates in Toys

June 11, 2014

Confidentiality Label

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For Forensic Use

Be First, Be Right, Be Agilent

For Forensic Use