Rashtreeya Sikshana Samithi Trust · 16MBT241 Pharmaceutical Biotechnology. 16MBT242 Agricultural Biotechnology. Elective ... 5 16MBT35 Internship / Industrial Training BT 0 0 3 0

Rashtreeya Sikshana Samithi Trust

R V College of Engineering(Autonomous Institution affiliated to VTU, Belagavi)

Master of Technology (M. Tech.)

Biotechnology

Scheme and Syllabus

2016

Department of Biotechnology M. Tech – Master of Biotechnology

R.V. College of Engineering, Bengaluru – 59(Autonomous Institution affiliated to VTU, Belagavi )

Department of Biotechnology

Vision: A premier department in Biotechnology Education, Research and Innovation with a focus on sustainable technologies

for the benefit of society and environment.

Mission: Create state-of-the-art infrastructure for research and training in Biotechnology. Develop graduates who are ethical and socially concerned. Promoting collaboration with academia, industries and research organizations at National and International level. Contribute to socioeconomic development through sustainable and inclusive technologies.

Program Educational Objectives (PEO)

M. Tech. in Biotechnology Graduates will be able to

PEO1. Have the ability to solve Biotechnology and Engineering problems, communication skills and team work that prepare them fora successful career in biotechnology and allied industries

PEO2. Function at a technically competent level in formulating and solving problems in biotechnology and develop an outlook forhigher education and lifelong learning.

PEO3. Gain the ability to comprehend, analyze, design and develop biological processes and products, exhibit professionalism,ethical attitude to become an entrepreneur

Program Outcomes (PO)

M. Tech. in Biotechnology Graduates will be able to:

PO 1. Scholarship of Knowledge: Use knowledge of principles and applications of Biotechnology.

Scheme and Syllabi – 2016 Admission Batch Page 2 of 47


PO 2. Critical Thinking: Design and formulate the solutions for various biotechnological challenges. PO 3. Problem Solving: Develop methodologies to solve the problems in the fields of health & pharma, bioprocess, and food &

Agriculture. PO 4. Research Skill: Acquire hands on skills sufficient to conduct research with minimal supervision. PO 5. Usage of modern tools: Apply advanced tools and techniques to the challenges of biotechnological sector. PO 6. Collaborative and Multidisciplinary work: Collaborate with the confluence of various domains of Biotech from academic,

industry and research institutes of national or international repute.PO 7. Project Management and Finance: Design and develop projects related to biotechnological and allied branches keeping

performance and cost constraints into consideration. PO 8. Communication: Inculcate inter and intra personal skills for effective communication.PO 9. Life-long Learning: Appreciate the breadth and depth of knowledge in biotech and the necessity for lifelong learning.

PO 10. Ethical Practices and Social Responsibility: Apply bio-engineering solutions to societal and ethical needs with focus onsustainability.

PO 11. Independent and Reflective Learning: Contemplate on real-time problems and develop independent interpretations.

Program Specific Outcomes (PSO)

M. Tech. in Biotechnology graduates will be able to:

PSO 1. Design practical and efficient solutions for production of biological compounds and improved biological systems byemploying modern Engineering tools and techniques.

PSO 2. Utilize professional skills to conduct research or industrial production in the areas of Health, Pharma, Food andAgriculture.

PSO 3. Provide eco-friendly solutions to Biotechnological problem within economic, environmental, societal and ethical constraints.



R. V. College of Engineering, Bengaluru – 59.(An Autonomous Institution affiliated to VTU, Belagavi)


M. Tech. in Biotechnology

FIRST SEMESTERSl.No

CourseCode

Course Title BoS CREDIT ALLOCATION TotalCreditsLecture

L

Tutorial

T

Practical

P

ExperientialLearning

S1 16MEM11P Project Management IEM 3 1 0 0 42 16MBT12 Advanced Biostatistics. BT 4 0 0 0 43 16MBT13 Transgenic Technology (Theory

and Practice)BT 4 0 1 0 5

4 16MBT14 Computational Biology. BT 4 0 0 1 55 16MBT15X Elective-1 BT 4 0 0 0 46 16MBT16 Professional Practice BT 0 0 2 0 2

Total 19 1 3 1 24Number of contact hours 19 2 6 4 31

Elective -116MBT151 Genomics, Proteomics and Microarray. 16MBT152 Enzyme Technology.



SECOND SEMESTERSl.No

CourseCode


L

Tutorial

T

Practical

P


S1 16MEM21R Research Methods. IEM 3 1 0 0 42 16MBT22 Upstream Processing (Theory

and Practice)BT 4 0 1 0 5

3 16MBT23X Elective-2 BT 4 0 0 0 44 16MBT24X Elective-3 BT 4 0 0 0 45 16MBT25X Elective-4 BT 4 0 0 0 46 16MBT26 Minor Project BT 0 0 5 0 5


Elective -216MBT231 Immunotechnology. 16MBT232 Nanobiotechnology.

Elective – 316MBT241 Pharmaceutical Biotechnology. 16MBT242 Agricultural Biotechnology.

Elective – 416MBT251 Recombinant DNA Products 16MBT252 Bioreactor Engineering



R. V. College of Engineering, Bengaluru – 59.(An Autonomous Institution affiliated to VTU, Belagavi)


M. Tech. Biotechnology

THIRD SEMESTERSl.No

CourseCode


L

Tutorial

T

Practical

P


S1 16MBT31 Downstream Processing &

Product RecoveryBT 4 0 1 0 5

2 16MBT32X Elective-5 BT 4 0 0 0 43 16MBT33X Elective-6 BT 4 0 0 0 44 16MBT34X Elective-7 BT 4 0 0 0 45 16MBT35 Internship / Industrial Training BT 0 0 3 0 36 16MBT36 Technical Seminar BT 0 0 2 0 2


Elective -516MBT321 Stem Cell and Tissue Engineering. 16MBT322 Food process technology.

Elective – 616MBT331 Human Diseases and Diagnostics. 16MBT332 Bioprocess Optimization, Modelling & Simulation.

Elective-716MBT341 Regulatory Affairs & Biobusiness. 16MBT342 Biodevices.



FOURTH SEMESTERSl.No

CourseCode


L

Tutorial

T

Practical

P


S1 16MBT41 Major Project BT 0 0 26 0 262 16MBT42 Seminar BT 0 0 2 0 2

Total 0 0 28 0 28



FIRST SEMESTER

ADVANCED BIOSTATISTICS

Course Code : 16MBT12 CIE Marks : 100

Hrs/Week : L:T:P:S:: 4:0:0:0 SEE Marks : 100

Credits : 4 SEE Duration : 3 HrsCourse Learning Objectives (CLO):1. Understand the fundamentals of statistics for biologists.2. Address the relevance of probability and statistics to advanced engineering problems in

biotechnology.3. Apply more advanced techniques to analyze data together with modeling of complex systems. 4. Design the biological experiments and its analysis based on the real data.5. Comprehended with statistical tools like SAS, R Analytics, IBM-SPSS and Minitab.

Unit – I 08Hrs

INTRODUCTION TO BIOSTATISTICS: Introduction to statistics, Measurement of data scale andCentral tendency, Data handling and statistical variables. Characteristics of biological data,Elementary theory of statistical errors. Measures of spread: range, percentile, variance and standarddeviation, Continuous random variables-normal and exponential distribution, discrete randomvariables-Bernoulli. Multivariate data, logarithmic transformations. Interpretation of biological dataand Significance of statistics to biological problems.

Unit – II 10HrsINFERENTIAL STATISTICS AND DISTRIBUTION: Basics of study design, Random blockdesign, stratified design; cohort studies, case-control studies, and odd ratio. Principles of statisticalinference: Parameter estimation, hypothesis testing. Statistical inference on categorical variables;categorical data and Single- and Double-blind experiments; Point and interval estimates; Samplingdistributions: Bivariate distribution-conditional and marginal distribution-Discrete distribution-Binomial, Poisson, geometric distribution-Continuous distribution, Normal, exponential and negativeexponential, gamma distributions-simple problems-properties.

Unit – III 10HrsSTATISTICAL TESTING: Null and alternative hypotheses, decision criteria, critical values, type Iand type II errors, Meaning of statistical significance; Power of a test; One sample hypothesis testing:Normally distributed data: z, t and chi-square tests; F-tests. Binomial proportion testing. Independentand dependent sample comparison, Wilcoxon Signed Rank Test, Wilcoxon-Mann-Whitney Test,Kruskal-Wallis test and Analysis of variance: One-way and Two-way Tables.

Unit – IV 10HrsSTATISTICAL CURVE FITTING: Correlation-Correlation coefficient, properties-problems onKarl Pearson and Spearman Rank correlation coefficient, Rank correlation-Regression equationsproblems-curve fitting by the method of least squares-fitting curves of the form ax+b,ax2 +bx+c,abxand axb - Bivariate correlation application to biological problems. Regression: simple linearregression; Least squares method; Multiple linear regression model, ANOVA table for multiple linearregression model, assessing model fit, polynomials and interactions. Optimization strategies with casestudies.

Unit – V 10Hrs



PREDICTIVE TOOLS IN STATISTICS: SAS® (Statistical Analysis System): overview of SASFoundation, SAS data sets, SAS functions, Data Management -sorting and grouping report data,Statistical Analysis-Algorithm and implementation using numerical methods with case studies.MINITAB: Introduction, Accessing Minitab, worksheet menu and session commands, data enteringand analyzing the simple formula for obtaining statistical inference. Application of computer foranalysis, interpretation and validation of statistical data.Expected Course Outcomes:COURSE OUTCOMESOn the completion of this course you will improve or gain capabilities in:1. Students are able to analyze and organize data graphically and numerically, interpret and conclude

the statistical problems.2. Students will improve your technical competence in designing research studies and analyzing data

pertaining to the biotechnological context. 3. Compare the utility of multivariate statistical methods in transcultural health research4. Students will able to analyze real-life datasets using statistical predictive techniques and tools.REFERENCE BOOKS: 1. Wayne W. Daniel , Chad L. Cross., “Biostatistics : Basic Concepts and Methodology for the

Health Sciences” Wiley India Pvt Ltd. 10th Edition, 2014. ISBN: 9788126551897.2. Shalabh Helge Toutenburg., “Statistical Analysis of Designed Experiments”, Wiley Publication,

Third Edition, 2010. ISBN-13: 978-14419114763. P.N Arora and P.K Malhan. “Biostatistics”, Himalaya Publishing House, 2013 ISBN: 81-8318-

691-2.4. T. Pullaiah, B. Ravindra Reddy, K. L. A. P Sarma., “Biostatistics”., Daya Publishing House,2013:

ISBN: 9788170359234.Scheme of Continuous Internal Evaluation (CIE):CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 markseach and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE(Theory) will be 100 marks.

Scheme of Semester End Examination (SEE):The question paper will have FIVE questions with internal choice from each unit. Each question willcarry 20 marks. Student will have to answer one question from each unit. The total marks for SEE(Theory) will be 100 marks.

Mapping of COs with PosPO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 H L H M M L L LCO2 H M H M M L LCO3 H H H M H H M L HCO4 H H H M H L L H

Mapping of COs with PSOs

CO1 CO2 CO3 CO4

PSO1 H H L MPSO2 L H H MPSO3 L L L L



Transgenic Technology




Hrs/Week : L:T:P:S 4:0:1:0 SEE Marks : 100

Credits : 5 SEE Duration : 3 hrsCourse Learning Objectives (CLO):Graduates shall be able to

1. Understand the mechanism of DNA replication, transcription, translation and gene regulation.2. Study the techniques of DNA and RNA isolations, and role of enzymes, vectors and hosts in

rDNA technology. 3. Acquire the various methods of genetic transformation of prokaryotes and eukaryotes. 4. Know various techniques involved in selection and screening of transformants. 5. Understand the importance of recombinant DNA technology for the production of use full

compounds and improved biological systems. Unit – I 10 Hrs

Central Dogma of Molecular Biology: Molecular structure of genes and chromosomes; Replication,transcription and translation in prokaryotes and eukaryotes. Gene regulation: Gene regulation andOperon concept, Constitutive, Inducible and Repressible systems; Operators and Regulatoryelements; Positive and negative regulation of operon; lac, trp, ara, his, and gal operons and theirregulation; Locus control regions. Structure and function of different types of RNA and mRNPs.RNAi technology: si RNA and miRNA mediated gene silencing, antisense technology. Genomeengineering using Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas systems.Regulation of Translation- global vs mRNA-specific. Translation inhibitors, Posttranslationalmodifications of proteins. Protein trafficking and transport.

Unit – II 10 HrsComponents: DNA and RNA: Isolation and purification of DNA (genomic and plasmid) and RNA.Manipulation of purified DNA, Chemical synthesis of DNA: Posporamidite method, use ofsynthesized oligonucleotides. Enzymes: Restriction endonucleases, ligases, polymerases andmodification enzymes; Vectors: plasmid, bacteriophage and other viral vectors, cosmids, Ti plasmid,Ri plasmids, yeast episomal plasmids (YEps), Yeast integrative plasmids (Yips), yeast replicativeplasmids, yeast artificial chromosome; mammalian and plant expression vectors. Host system:Microbes, plant and animal cells.

Unit – III 9 HrsGenetic Transformation: Introduction of DNA into living cells: preparation of competent cells,methods of genetic transformation: Heat shock and electroporation method for microbes, Chemical,physical and biological methods for plants, and animals. Introduction of phage DNA into bacterialcells, identification of recombinant phages, transformation of non-bacterial cells. Identification ofrecombinants in prokaryotes and eukaryotes. Generation of genomic and cDNA libraries. Obtain aclone of specific gene: Nucleic acid hybridization, colony and plaque hybridization probing,

Unit – IV 9 HrsScreening of Transgenics: Isolation and amplification of specific nucleic acid sequences, PCR, RTPCR and qRT PCR, DNA sequencing methods, strategies for genome sequencing. Methods foranalysis of gene expression at RNA and protein level, Southern, Northern and Western hybridization.large scale expression, such as micro array based techniques. Analysis of DNA polymorphism: RFLP,RAPD and AFLP techniques. Studying gene expression and function. In vitro mutagenesis anddeletion techniques, gene knock out in bacterial and eukaryotic organisms.

Unit – V 10 Hrs



The applications of transgenic technology: Molecular cloning of DNA or RNA fragments inbacterial and eukaryotic systems. Production of heterologous proteins from cloned genes in E.coli,Saccharomyces cerevisiae, Pichia pastoris, Baculovirus-insect cell and mammalian cells. Productionof recombinant pharmaceuticals: Insulin, human growth hormone, vaccines. Identification of genesresponsible for human diseases. Gene therapy, Production of biotic or abiotic resistant plants, highyielding plants, recombinant protein production in plants. Cloning livestock by nuclear transfer.Ethical issue with genetically modified organisms.

Unit – VI (Lab Component)1. Isolation and purification of genomic DNA from prokaryotic/ eukaryotic cells.2. Isolation and purification of plasmid DNA.3. Isolation and purification of total RNA. 4. Restriction digestion of DNA.5. Constructing recombinant DNA using gene of interest and vector.6. Preparation of competent cells of E.coli and genetic transformation of E.coli.7. Blue and white screening of transformants, confirmation of transformants using colony PCR. 8. Agrobacterium mediated genetic transformation of plants.9. Amplification of DNA fragments using PCR.10. Extraction of proteins from transformed prokaryotic cells.

Expected Course Outcomes:After going through this course the student will be able to:CO1: Define and explain mechanism of DNA replication, transcription, translation, gene regulation

and process of recombinant DNA technology.CO2: Apply techniques required for recombinant DNA technology in prokaryotes and eukaryotes. CO3: Analyze and evaluate the recombinant proteins and genetically modified organisms. CO4: Design a protocol for the production of heterologous proteins and genetically modified

organisms. Reference Books:

1. Green MR, Sambrook J, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press; 4th edition, 2012, ISBN-10: 1936113422.

2. Glick BR, Pasternak JJ, and Patten CL, Molecular Biotechnology – Principles and applicationsof recombinant DNA, ASM Press, 4th Edition. 2010. ISBN:978-1-55581-498-4.

3. Brown T.A. Gene Cloning and DNA Analysis – An Introduction, Wiley-Blackwell Science, 6 th

Edition, 2010, ISBN: 9781405181730.4. Lodish H, Berk A, Kaiser CA, Krieger M, Scott MP, Bretscher A, Ploegh H and Matsudaira P,

Molecular Cell Biology, Freeman, 8th Edition, 2016, ISBN-13: 978-1464183393.5. Pinkert CA, Transgenic Animal Technology: A Laboratory Handbook, Elsevier, 3rd edition,

2014, ISBN-13: 978-0124104907.


https://www.amazon.com/s/ref=dp_byline_sr_book_1?ie=UTF8&text=Carl+A.+Pinkert&search-alias=books&field-author=Carl+A.+Pinkert&sort=relevancerank

https://www.amazon.com/s/ref=dp_byline_sr_book_2?ie=UTF8&text=Joseph+Sambrook&search-alias=books&field-author=Joseph+Sambrook&sort=relevancerank

https://www.amazon.com/Michael-R.-Green/e/B00DWAGKLI/ref=dp_byline_cont_book_1


Scheme of Continuous Internal Evaluation (CIE):CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 markseach and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE(Theory) will be 100 marks.Scheme of Semester End Examination (SEE):The question paper will have FIVE questions with internal choice from each unit. Each question willcarry 20 marks. Student will have to answer one question from each unit. The total marks for SEE(Theory) will be 100 marks.Scheme of Continuous Internal Evaluation (CIE) for Lab:CIE for the practical courses will be based on the performance of the student in the laboratory, everyweek. The laboratory records will be evaluated for 40 marks. One test will be conducted for 10 marks.The total marks for CIE (Practical) will be for 50 marks.Scheme of Semester End Evaluation (SEE) for Lab:SEE for the practical courses will be based on conducting the experiments and proper results for 40marks and 10 marks for viva-voce. The total marks for SEE (Practical) will be 50 marks.Mapping of COs with POs and PSOs

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PSO1 PSO2 PSO3CO1 M M L L L - - L M - -CO2 H H M M M - - M M M -CO3 H H M M M - - M M M -CO4 H H H M H - - M M H -

Mapping of Cos with POs

PSO1 PSO2 PSO3CO1 L L LCO2 H M MCO3 H M LCO4 H H H



Computational Biology




1. Study domain specific Mathematical and Statistical modeling applications and study the role of computer science in lifesciences.

2. Acquire knowledge of Pattern, Motif and Signal recognition and processing3. Study role of Computational techniques in the field Physical, Cytogenetic and Linkage

mapping of gene/genome resources4. Understand the importance of Information Technology and Stochastic as well as Numerical

approaches to solve the problems related to High throughput Data in Cancer Research5. Explore practical applications of computational biology in the area of Immuno-informatics

Unit – I 10Hrs

Statistical approach to DNA and Protein sequence analysis: Introduction, scope and applicationsof Computational biology. Molecular Biology databases. Analysis of single DNA sequence: shotgunsequencing, DNA modeling, Scanning long repeats, Analysis of patterns and Counting of overlaps.Analysis of Multiple DNA or Protein sequences: Frequency comparisons of two sequences. Simpletests for significant similarity in an alignment. Alignment algorithms for two sequences: Gappedglobal comparisons and Dynamic programming algorithms, linear gap model for fitting one sequenceinto another and local alignment.

Unit – II 08Hrs

Patterns, Motifs and Signals: Pattern matching - Pattern matching with Consensus sequencesQuantitative & Probabilistic pattern matching. Structural domains and Motifs - Sequence blocks &Profiles, Protein sequence motifs, Protein structural motifs, Clustering and Functional analysis ofcoordinately regulated genes. Discovering transcriptional regulatory Signals, Ultra-conservation inthe Human Genome.

Unit – III 09Hrs

Restriction mapping, Map assembly and Sequencing – Algorithms for restriction mapping,shotgun sequencing, DNA sequencing, Human Genome Project. DNA Arrays, Sequence Comparison– sequence alignment, tuples, antichain. Finding signals in DNA - Gibbs sampling, Viterbi algorithm,Hidden Markov Models in Bioinformatics, Computational Proteomics (amino acid, C-terminal,directed acyclic graph), Problems -circular permutation, interval graph.

Unit – IV 10Hrs

Computational Biology and Cancer research: Mathematical modeling of tumorigenesis - Cellularautomaton, tumor, angiogenesis. One hit and two hit stochastic models - Tumor suppressor gene,Kolmogorov forward equation, and retinoblastoma. Microsatellite and chromosomal instability insporadic - APC gene, colorectal cancer, point mutation. Chromosome loss. Basic models of tumorinhibition and promotion - Metastatic, Angiogenic tumor cells, Angiogenesis inhibition. Mechanismsof tumor neovascularization - vasculogenesis, Cancer and Immune responses - Dendritic cellvaccination, Viruses as antitumor weapons - Tumor load, Viral replication and Oncolytic viruses.



Unit – V 10Hrs

Computational Immunology: Overview of immune system, Introduction to computationalimmunology Immunological databases – IMGT – IMGT-GENE-DB, – IMGT-HLA, Tools for theprediction binding affinity between peptide : TAP:MHC:TCR- MHC: Peptide Binding Prediction -SYFPEITHI, BIMAS, MHC PRED, - Future of computational modeling and prediction systems inclinical immunology -overview of models- models for HIV infection.

Expected Course Outcomes:After going through this course the student will be able to:CO1: Define and explain concepts of Sequence, Protein Structure Hierarchy, Physical and Virtual

mapping of Biological dataCO2: Apply Pattern recognition and Processing along with Pattern prediction techniques to solve the

problems in the area Proteomics, Genomics, and informatics Cancer biology as well asImmunology

CO3: Analyze High Throughput Data generated by sequencing/mapping/hybridization and evaluateusing Clustering and searching algorithms with case studies

CO4: Design and execute protocols to perform high throughput data analysis in the field ofProteomics, Genomics, and informatics Cancer biology as well as Immunology

Reference Books:

1. Hamid R Arabnia, Quoc Nam Tran. Emerging Trends in Computational Biology, Bioinformatics,and Systems Biology: Algorithms and Software Tools, Morgan Kaufmann, 2015, ISBN9780128026465

2. Röbbe Wünschiers. Computational Biology: Unix/Linux, Data Processing and Programming,Springer Science & Business Media, 2012, ISBN 9783642185526

3. Lukas K. Buehler, Hooman H. Rashidi. Bioinformatics Basics: Applications in BiologicalScience and Medicine, CRC Press, 2005, ISBN 9780849312830

4. Darren Flower, Jon Timmis. In Silico Immunology, Springer Link, 2007, ISBN: 978-0-387-39238-7

5. Dominik Wodarz, Natalia Komarova. Computational Biology of Cancer: Lecture Notes andMathematical Modeling, World Scientific, 2005, ISBN 9789814481878

6. Neil C.Jones and Pavel .A Pevzner. An introduction to Bioinformatics Algorithms(Computational Molecular Biology), MIT Press, 2004, ISBN 0-262-10106-8

Scheme of Continuous Internal Evaluation (CIE):CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 marks each and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE (Theory) will be 100 marks.Scheme of Semester End Examination (SEE):The question paper will have FIVE questions with internal choice from each unit. Each question will carry 20 marks. Student will have to answer one question from each unit. The total marks for SEE (Theory) will be 100 marks.Mapping of COs with POs and PSOs

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11CO1 H M M L L MCO2 M H H M H H L L LCO3 M H M L H M M MCO4 L M L H M M M M


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CO1 CO2 CO3 CO4

PSO1 L H M HPSO2 L H H MPSO3 L L L



Genomics, Proteomics and Microarray




1. Understand the organization of prokaryotic and eukaryotic genomes, databases and sequencing techniques

2. Acquire molecular insight into the tools and techniques used in genome analysis.3. Get an overview of the different protein purification and sequencing techniques4. Understand the techniques involved in the identification and characterization of all

the proteins synthesized in a cell or a tissue. 5. Understand the basic principles of DNA and Protein microarray

Unit – I 10 Hrs

Introduction to Genomics: Genome evolution and organization in prokaryotes and eukaryotes,Genome mapping: Genetic and physical mapping. Molecular markers and protein markers,Genome sequencing, basics, strategies and methodology. Comparative and Functional genomics;Model systems- Arabidopsis, Human, Drophila and E coli. Serial analysis of gene expression(SAGE) and targeting induced local lesions in genome (TILLING).Biological databases; Primaryand secondary for nucleic acid and proteins, structural databases, metabolic pathways andspecialized databases. Genome Wide Association Studies (GWAS)

Unit – II 10HrsTools for genomics ; Computational analysis of sequences- finding genes and regulatory regions;Gene annotation; Similarity searches; Pairwise and multiple alignments; Alignment statistics;Prediction of gene function using homology, context, structures. Expression sequence tags (ESTs), Microarrays technology- Principles and applications, FISH, transcriptome analysis and SNPsdetermination. Allele mining and single nucteotide polymorphisms (SNPs).Transcriptomics;Cancer Genomics, Epigenomics, Chemical Genomics; Metabolomics, Nutrigenomics,interactomics, Metagenomics. Personal Genomics; Social, Legal and Ethical Implications ofHuman Genome Research.

Unit – III 9HrsIntroduction and scope of proteomics, Protein separation techniques: Ion exchange, Size exclusionand affinity chromatographic techniques, Poly acrylamide gel electrophoresis, isoelectric focusing,two dimensional poly acrylamide gel electrophoresis, Mass spectrometry based techniques forprotein identification.

Unit – IV 10HrsProtein sequencing- Edman degradation, mass fingerprinting, protein synthesis and posttranslational modifications. Identification of phosphorylated proteins, characterization of multi-protein complexes, protein - protein interactions and quantitative proteomics- Characterization ofinteraction clusters using two-hybrid systems. Protein arrays-definition, applications- diagnostics,expression profiling, Functional proteomics, Protein structure analysis, Protein databases, Clinicaland biomedical applications of proteomics.



Unit – V 09HrsMicroarray techniques: Importance and applications of microarray techniques in biotechnology,Types – Single and multiple approaches. Challenges of microarray technology. Microarray Probepreparation, hybridization, Image processing, Transformation of expression ratio and datanormalization. Low and high level information Analysis – Data Preprocess of Chemicalcompounds Microarray, Biomolecular microarray - Protein and proteomics Microarray, DNAMicroarray, MicroRNA Population, Cellular and tissue microarray. Microarray Database for SerialAnalysis of Gene Expression, Gene Expression Omnibus of NCBI, Array Express of EMBL andAntibodies Arrays on Miniature Western Blots methods.Expected Course Outcomes:After going through this course the student will be able to:CO1: Understand the construction concepts of various genome maps and large scale sequencingCO2: Develop diagnostic tools for plant, animal and human diseasesCO3: Apply proteomics to solve complex biological problems regardless of types of organism. CO4: Understand the basic concepts of microarrays and analyze the differential gene expression.Reference Books:1. Sandor Suhai, Genomics and Proteomics: Functional and Computational aspects, Kluwer

academic publishers, 2007, ISBN: 97803064682302. Liebler,D.C. Introduction to Proteomics: Tools for the New Biology, Humana Press,

2002. ISBN-13: 978-08960399263 R.M. Twyman, Principles of Proteomics, garland Science/BIOS Scientific publishers, 2004,

ISBN-10: 1-85996-273-4.4 Steven Russell, Lisa A. Meadows and Roslin R. Russell. Microarray Technology in Practice:

2nd Edition, Academic Press, 2013. ISBN 13: 978-0-12-372516-5Scheme of Continuous Internal Evaluation (CIE):CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 markseach and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE(Theory) will be 100 marks.Scheme of Semester End Examination (SEE):The question paper will have FIVE questions with internal choice from each unit. Each questionwill carry 20 marks. Student will have to answer one question from each unit. The total marks forSEE (Theory) will be 100 marks.Mapping of COs with POs

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11CO1 H M M L L - - M M L LCO2 H H H H M - - M M L LCO3 H H H H M - - M M L LCO4 H M M L L - - M M L L

Mapping of COs with PSOsPSO1 PSO2 PSO3

CO1 L M L

CO2 M M M

CO3 M M M



CO4 L M L

ENZYME TECHNOLOGY



Credits : 4 SEE Duration : 3 hrsCourse Learning Objectives (CLO):Graduates shall be able to1) Get an overview of the basics of enzyme technology and its applications in different fields of

industry2) Learn about the different methods of characterization of enzymes3) Get an insight of different types of enzyme inhibitions and their industrial and clinical

applications4) Understand different techniques of enzyme immobilization and their industrial and

environmental applications5) Appreciate the industrial Applications of Enzyme Technology and explore case studies of

enzymes that were engineered for industrial and medical applications

Unit – I 8 Hrs

Introduction- Nomenclature and classification, Application of enzymes in process industries andhealth care. Enzymes as biocatalysts: advantages and disadvantages over chemical catalysts andcharacteristics, microbial production and purification of enzymes, Assay of enzyme activity andspecific activity, Coenzymes, Cofactors.

Unit – II 12HrsEnzyme-Ligand interaction, collision theory and transition state theory and role of entropy incatalysis, Methods for investigating the kinetics of Enzyme catalyzed reactions – Initial velocitystudies, End point, Steady state and Pre steady state Kinetics, Estimation of Michaelis-Mentenparameters, kinetics of single and multisubstrate enzymes catalyzed reactions. Allostericity andcooperativity. Effect of pH and temperature on enzyme activity. Numericals in enzyme kinetics.

Unit – III 10HrsEnzyme inhibition- irreversible and reversible competitive, noncompetitive, uncompetitive, mixed,inhibitions. Kinetic differentiation and graphical methods. Examples. Determination of inhibitorconstant, therapeutic, diagnostic and industrial applications of enzyme inhibitors. Numericals inenzyme inhibition.

Unit – IV 10Hrs



Techniques of enzyme immobilization; kinetics of immobilized enzymes, effect of solute, activity& kinetics of immobilized enzymes; applications of immobilized enzyme technology-: Enzymesensors for clinical analysis, therapeutic medicine, Environmental applications. Economicargument for immobilization. Case studies on therapeutic enzymes.

Unit – V 8HrsIndustrial Applications of Enzyme Technology: Textile industry, detergents, pulp and paper,leather, wood, animal feed, food and dairy industry - amylases, proteases, lipases, pectinases.Enzyme Engineering: Glucose isomerase, subtilisin, redesigned lactate dehydrogenase. Syntheticenzymes- peroxidase. Catalytic antibodies. Challenges in enzyme technology

Course Outcomes:1) Summarize current process involved in industrial enzyme production from protein

production to purification and formulation2) Understand the different types of enzyme inhibition and their physiological implications as

well as their medical and environmental applications3) Compare and contrast the historical uses of enzyme technology with current application in

diverse range of industries4) Research a contemporary application of enzyme technology and present the results with

well structured oral presentation

Reference Books:

1) Klaus Buchholz, Volker Kasche and Uwe Theo Bornscheuer. Biocatalysis and Enzyme Technology. 1st edn. Wiley-VCH, 2005.

2) Wolfgang Aehle. Enzymes in industry-production and applications. 3rd edn. Wiley-VCH, 2007.3) Chaplin M.F. and C. Bucke. Enzyme Technology. CUP. Cambridge. 1990.4) Price N. C. and L Stevens. Fundaments of Enzymology: 3rd edn. Oxford University Press. 2003.5) Bioprocess Engineering: Basic Concepts (2nd Edition), Shuler and Kargi, Prentice Hall

International.2002

Scheme of Continuous Internal Evaluation (CIE):

Scheme of Semester End Examination (SEE):

Scheme of Continuous Internal Evaluation (CIE) for Lab:

Scheme of Semester End Evaluation (SEE) for Lab:

Mapping of COs with POsPO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 H M M M L L -- -- L -- LCO2 M H M M L M -- -- L -- L



CO3 M H M M L M -- -- L -- LCO4 M H M M M M -- -- M -- L


CO1 H L LCO2 M L MCO3 L M MCO4 M H H



UPSTREAM PROCESSING

Course Code : 16MBT21 CIE Marks : 100+50

Hrs/Week : L:T:P:S :: 4:0:1:0 SEE Marks : 100+50

Credits : 5 SEE Duration : 3 HrsCourse Learning Objectives (CLO):Graduates shall be able to

1. Know the concept of preparation and production of industrial bio-products by using microbes,Plant and animal sources.

2. Describe the role of preparation and optimization of media in production of bio-products 3. Comprehend different process controllers and various parameters to be controlled during

Fermentation.4. Design the Bioreactors and its aspects for plant, animal and microbial growth. 5. Learn the cost estimation and market value of biological processes.

Unit – I 10Hrs

Introduction to upstream process technology: Typical fermentation flowsheets for plant, animaland microbial processes. Advance culturing techniques for plant and animal cells. Growth kinetics ofmicrobial, plant and animal cells in Bioreactor. Specialized animal cell culturing techniques forproduction of immune based compounds. Different modes of operating the upstream processes inbatch, fed batch and continuous operations. Production of industrial important compounds frommicrobes, plant and animal-metabolites, antibodies, vaccines, enzymes, rDNA proteins andbiotransformation molecules.

Unit – II 08Hrs

Media and sterilization: Stoichiometric growth equation for various biomass and simple products –ethanol. Raw material selection and media formulations with examples. Media optimization usingstatistical methods-Plackett Burman, Central Composite Design and Response Surface and risingridge methods. Sterilization: Batch, continuous (Direct and indirect) and filter sterilization for plantand animal media.

Unit – III 10Hrs

Instrumentation in Bioreactors: Microbial bioreactors-fully automated and sterializable fermenters.Instruments required to monitor the various process parameters Raw material and media formulationfor fermentation process: Fermentation media- Measurement of temperature, pressure, pH, DissolvedOxygen, foam, product activity, substrate concentration and critical components and agitation control.Measurement of flow rate of liquid and gases; online estimation of process parameters by SCADAand case study on SCADA.

Unit – IV 10Hrs

Design of a Bioreactor: Functions, construction materials, and maintenance of aseptic conditions of abioreactor. Types of fermenters: Membrane, animal bioreactors, plant bioreactors, fluidized bed,Immobilized, packed bed bioreactors and disposable bio reactors. Design of jacketed vessel for batchand continuous processes. Calculation of thermodynamic aspects for SS416, SAE40 grade andborosilicate fermenters. Scale up and scale down methods for fermentation, good manufacturing



practices, Estimation of OTR by static and dynamic method. Computer applications in fermentationtechnology.

Unit – V 10Hrs

Upstream economics and effluent treatment: Unit operations involved in upstream processing ofvarious compounds generated from fermentation industry. Cost estimation and development ofbalance sheet for various upstream processes. Market value for biotechnological compounds. Effluenttreatment: characteristics of the effluent generated from upstream processes. Physical, chemical andbiological methods of treating the effluents. Case study: Very high value and low volume compoundwith examples for cost estimation.

Expected Course Outcomes:After going through this course the student will be able to:CO1: Remember and understand the processes for cultivating the cultures, for production of various bio-products. CO2. Implement the techniques for Upstream Processing and its parameters optimization CO3. Analyze the scale up techniques, process economics and effluents management.CO4. Design of bioreactors involved in upstream processing.

PRACTICALS1. Cultivation of yeast in fermenter and to produce the ethanol. 2. Production and extraction of secondary metabolites from callus culture.3. Isolation of primary cell lines and its maintenance. 4. Scaling-up production of wine from microbes. 5. Production of antibiotics from bacterial and fungal species, its inhibition studies.6. Estimation of metabolic respiration rate of yeast. 7. Bio-synthesis of Pectinase and its purification from microbes. 8. Production of proteases from microbes and estimation of its activity.9. Production and estimation of citric acid by microbes.10. Production of lactic acid and its quantitative estimation using bacterial culture.

Reference Books:

1. Brian McNeil and Linda Harvey, “Practical Fermentation Technology”, Wiley publishers, 2008.ISBN: 0470725281

2. Peter F. Stanbury, Allan Whitaker, Stephen J Hall, “Principles of Fermentation Technology”, 2ndedition pergamon press. ISBN: 0080946437.

3. Pauline M. Doran, “Bioprocess engineering principles”, 2d edition, academic press, 2012. ISBN:978-0-12-220851-5.

4. Michael Butler, “Cell Culture and Upstream Processing”, 1st edition, Taylor & Francis, 2013.ISBN: 978-0415399692.


http://as.wiley.com/WileyCDA/Section/id-302477.html?query=Linda+Harvey

http://as.wiley.com/WileyCDA/Section/id-302477.html?query=Brian+McNeil


Scheme of Continuous Internal Evaluation (CIE):

CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 marks each and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE (Theory) will be 100 marks.Scheme of Semester End Examination (SEE):

The question paper will have FIVE questions with internal choice from each unit. Each question will carry 20 marks. Student will have to answer one question from each unit. The total marks for SEE (Theory) will be 100 marks.Scheme of Continuous Internal Evaluation (CIE) for Lab:CIE for the practical courses will be based on the performance of the student in the laboratory, everyweek. The laboratory records will be evaluated for 40 marks. One test will be conducted for 10 marks.The total marks for CIE (Practical) will be for 50 marks.Scheme of Semester End Evaluation (SEE) for Lab:SEE for the practical courses will be based on conducting the experiments and proper results for 40marks and 10 marks for viva-voce. The total marks for SEE (Practical) will be 50 marks.Mapping of COs with POs

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11CO1 H H M M M H M L H L LCO2 L H H M H L H L M L LCO3 L L M M L H H L H L HCO4 H H H M L L M L M L L


CO1 CO2 CO3 CO4PSO1 L M H HPSO2 L M H HPSO3 L L H L



IMMUNOTECHNOLOGY



Credits : 4 SEE Duration : 3 HrsCourse Learning Objectives (CLO):Graduates shall be able to

1. Understand the mechanism of immune response and reactions2. Utilize various components and assets required for immune reaction3. Comprehend structure of immunoglobulin and antibody4. Apply various techniques for understanding intricacies of immunology5. Figure out various tools and mechanism for graft rejection reactions

Unit – I 9 Hrs

Introduction to Immune System, organs, cells and molecules involved in innate and adaptiveimmunity.Hematopoiesis and its regulation, role of cytokines, chemokines and leukotrienes,phagocytosis and microbicidal mechanisms. Immediate hypersensitivity: role of eosinophils, and mastcells. Genetic bases of immune response – Heterogenecity; Immune modulators;

Unit – II 10 HrsReceptors of innate immunity: Toll-like receptors, opsonization, Immunochemistry of Antigens -Immunogenicity, Antigenicity, haptens, super antigens , Toxins-Toxiods, Hapten carrier system. B andT cell epitopes, T cell receptors, Activation of T cells, APC-T cell interaction, Differentiation andactivation of B cells, BCR and editing, Major Histocompatibility Complex: genetic organization ofH2 and HLA complexes. Class I and class II MHC molecules, MHC restriction. Antigen processingand presentation pathways

Unit – III 9 HrsAntibody structure and function: Classification of immunoglobulins, immunoglobulin domains,concept of variability, isotypes, allotypes and idiotypic markers. Antigen-antibody interactions,Immunoglobulin genes, VJ/VDJ rearrangements and genetic mechanisms responsible for antibodydiversity,Class switching, soluble forms of immunoglobulin, The complement system: classical andalternative pathways,

Unit – IV 9 HrsHybridoma, monoclonal antibodies, and antibody engineering, Immunological Techniques antibodygeneration, detection of molecules using ELISA, RIA, Western blot, immunoprecipitation,flowcytometry, immunofluorescence microscopy, Insitu localization techniques such as FISH,GISH,Monoclonal antibodies and their role in cancer.

Unit – V 10 HrsApplication of immunological principles- Transplantation immunology- immunologic basis of graftrejection, clinical manifestations, immunosuppressive therapy, Immunostimulants, Vaccines: types,recombinant vaccines and clinical applications, Tumor immunology and autoimmunity, Expected Course Outcomes:After going through this course the student will be able to:CO1: Apprehend the concepts of immunity and immune reactions.CO2: Analyze the various types of immune responsesCO3: Apply the knowledge of immunology to identify various immunological reactions and

interactions



CO4: Evaluate the significance and applications of various immunological techniques.Reference Books:

1. Ashim K. Chakravarthy. Immunology and Immunotechnology Oxford University Press. 2006.

2. T. Kindt, R. Goldsby, B. A. Osborne, Kuby Immunology, W. H. Freeman, 6th edition, 2006.

3. Tizard. Immunology – an Introduction, Thomson. 2004.

4. Roitt, I. Essential Immunology by Blackwell Scientific Publications, Oxford. 2001

5. Benjamini E. and Leskowitz S. Immunology: A short course, Wiley Liss, NY. 2003.Scheme of Continuous Internal Evaluation (CIE):CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 markseach and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE(Theory) will be 100 marks.Scheme of Semester End Examination (SEE):The question paper will have FIVE questions with internal choice from each unit. Eachquestion will carry 20 marks. Student will have to answer one question from each unit. Thetotal marks for SEE (Theory) will be 100 marks.Mapping of COs with Pos

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 H H M L H M M M L M LCO2 H H M M M H H H L H LCO3 H M M M M H H H L H LCO4 H H H M H H H H L H M


PSO 1 PSO 2 PSO 3CO 1 H M LCO 2 M M LCO 3 H H HCO 4 H H H



NANOBIOTECHNOLOGY



Credits : 4 SEE Duration : 3 HrsCourse Learning Objectives:

Graduates shall be able to1. Understand the fundamentals of nanomaterials.2. Describe methods for their synthesis, characterization and their applications 3. Characterize & differentiate various biosensors & explain their functioning under different

conditions. 4. To have awareness about the nanosensors used in diagnostic and therapeutic use.5. To design a concept for a nanoscale product and their applications in medical field.

Unit – I 8 Hrs

Fundamentals of Nanoscience and Engineering: History, Types of nanomaterials: Fullerenes,Nanoshells, Quntum dots, Dendrimers, Nanofibers, Approaches of Fabrication: Top-Down andBottom-up methods of nanofabrication and Nanosynthesis. Nanolithography: hard and softlithography. Characterization of nanomaterials using spectroscopic (UV-Vis, FTIR and Raman) andmicroscopic methods (SEM, TEM, STM and AFM).

Unit – II 10 HrsNanobiomaterials: Biosynthesis of Nanoparticles, Microbial Nanoparticle productionBiomineralization, Magnetosomes, Nanoscale magnetic iron minerals in bacteria, virus & fungi. DNAbased Nano structures. Protein based Nano structures. Introduction-Biocompatibility – anti bacterialactivity – principles involved – Applications. Biomaterial nanocircuitry; Protein based nanocircuitry;Neurons for network formation. DNA nanostructures for mechanics and computing and DNA basedcomputation; DNA based nanomechanical devices. Function and application of DNA basednanostructures. Bionanomaterials in Nature: Lotus leaf as a model self cleansing system. Geckofoot as a case study for biological generation of adhesive forces. Diatoms as an example for siliconbiomineralization. Mussel inspired nanofiber for tissue engineering. Biomechanical strengthproperties of Spider silk.

Unit – III 9 HrsMicro & Nano Electromechanical systems and Microfluidics: BioMEMS/BioNEMS: Types oftransducers: mechanical, electrical, electronic, magnetic and chemical transducers. Nano sensors:Types: Electronic nose and electronic tongue, magnetic nanosensors. mechanical nanosensors:Cantilever Nanosensors, Microfludics: Laminar flow, Hagen-Peouiselle equation, basic fluidideas, Special considerations of flow in small channels, micro mixing, microvalves & micropumps,Body on a chip and lab on a chip.

Unit – IV 10 HrsNanosensors: Nanofabricated devices to separate and interrogate DNA, Interrogation of immune andneuronal cell activities through micro- and nanotechnology based tools and devices. Types ofNanosensors and their applications. Electromagnetic nanosensors: Electronic nose and electronictongue, Magnetic nanosensors. Mechanical nanosensors: Cantilever Nanosensors, NanoBiosensors:NanoBiosensors in modern medicine.

Unit – V 10 Hrs



Medical Nano biotechnology in Diagnostics, therapeutics, drug delivery, Nano Surgery and TissueEngineering . Drug Delivery Applications, Bioavailability, Sustained and targeted release. Benefits ofNano drug delivery system. Use of Microneedles and nanoparticles for targeted and highly controlleddrug delivery. Nano robots in drug delivery and cleaning system. Design of nanoparticles for oraldelivery of peptide drugs. Nanotoxicity assessment: In-vitro laboratory tests on the interaction ofnanoparticles with cells.Expected Course Outcome: After going through this course the student will be able to:CO1: Understand and apply the knowledge of nanomaterials and nanobiomaterials to enable health

sector advancements. CO2. Interpret and apply the techniques of manufacturing and characterization processes. CO3. Apply the knowledge for various applications in Biomedical field.CO4. Design devices and systems for various biological applications

Reference Books:

1. Gabor L. H., Dutta J., Tibbals H. F., Rao A., Introduction to Nanosciences, 2008, CRC press,ISBN- 1420048058

2. Murty B.S., Shankar P., Raj,B., Rath, B.B. and Murday, J. Textbook of Nanosciences andNanotechnology, 2013, Springer, Co-publication with University Press (India) Pvt. Ltd. VCH,XII. ISBN- 978-3-642-28030-6.

3. Niemeyer and C.A. Mirkin, Nanobiotechnology: Concepts, Applications and perspectives,Wiley –VCH, 2 edition, 2013.ISBN -978-3-527-30658-9.

4. Vinod kumar Khanna, Nanosensors: 2013, Physical, Chemical and Biological, CRC press,ISBN 9781439827123

5. Sandra J. Rosenthal, David W. Wright, NanoBiotechnology Protocols, Springer (1st edition,2005, ISBN-10 1588292762), 2nd edition, Humana Press, 2013. ISBN- 13 978-158829276

Scheme of Continuous Internal Evaluation (CIE):CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 markseach and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE(Theory) will be 100 marks.Scheme of Semester End Examination (SEE):The question paper will have FIVE questions with internal choice from each unit. Each question willcarry 20 marks. Student will have to answer one question from each unit. The total marks for SEE(Theory) will be 100 marks.Mapping of COs with POs and PSOs

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PSO1 PSO2 PSO3CO1 H M M M L H - - H H H H L M

CO2 M H H M H M M - M H M H - M

CO3 H H H M H M M - M H H H - M

CO4 H H H M H H M - M M M H - M


CO1 H L MCO2 H - MCO3 H - MCO4 H - M



Pharmaceutical Biotechnology



Credits : 04 SEE Duration : 100Course Learning Objectives (CLO):Graduates shall be able to

1. To enable students to develop an appreciation and understanding to the pharmaceutical research and development.

2. Determine parameters related to stability and formulation of biotechnology products 3. Discuss drug dose relationship and drug mechanism of reactions in the human being. 4. Understand and evaluate the different pharmaceutical products and their effects on mankind.5. Evaluate various pharmaceutical products and their applications through appraisal of both benefits of

mankind and ethical issues.Unit – I 09 Hrs

INTRODUCTION: Configuration and conformation of drug molecules, rational drug design,various approaches in drug discovery, drug targets and pharmacophores. Physical properties of drugs- physical form, polymorphism, particle size, shape, density, wetting, dielectric constant, solubility,dissolution, organoleptic property and their effect on formulation. DRUGS & COSMETIC ACT.cGMP concepts – Development, Manufacturing Record, Analytical & process Validation, Equipment& utilitiy Qualification and Calibration, Personnel procedures; Regulatory bodies & requirements -Indian FDA, WHO GMP,U.S FDA. Schedule-Y. Pre-clinical study requirements, clinical trial phases,Types of trials and Bioethics, Bioavailability and Bio equivalance studies. PHARMACOPOEIA.

Unit – II 08 HrsMolecular Modeling in Drug Discovery: Drug discovery process, Lipinski “rule of 5”, Partitioncoefficient, Hammet contant, Hansch analysis. Role of Bioinformatics in drug design. Targetidentification and validation, lead optimization and validation, Structure and ligand based drugdesign, Modeling of target-small molecule interactions, Molecular Simulations, Protein modeling.Structure Activity Relationship - QSARs and QSPRs, QSAR Methodology, Various Descriptors usedin QSARs: Electronic

Unit – III 10 HrsDrug Pharmacokinetics and Pharmacodynamics: Principles of basic and clinicalpharmacokinetics and pharmacodynamics. Physiology of the absorbing membranes. Mechanisms ofdrug absorption - passive and active transport - Fick’s first law - affect of membrane permeability onoral absorption. Factors affecting bioavailability-Physiological, Adverse drug reactions. Druginteractions, Bioassay of drugs and biological standardization of immunoagents.Routes: Oral, Sublingual, Buccal, Parenteral, Topical, Rectal and Inhalation. The pharmacokineticimplications of various routes of administration- Advantages and Disadvantage of various routes ofadministration.

Unit – IV 09.HrsIntroduction to Vaccinology Classification, active immunization, vaccines technology, perspectivevaccines, means of passive immunization, antibodies in therapy, antibody engineering, monoclonalantibodies, immunoconjugates - specific drug targeting, immunotoxins. Immuno-Therapeutics: Overview of drug discovery and development of immuno-drugs. Cytokinesclassification, pathways of activation, Therapeutic use of cytokines. Immunomodulators



classification, thymic hormones and synthetic immunostimulators. Compliment pathwaysdiagnostics, ELISA, Flow cytometry, ELISPOT, immnuno radiology, Basic immunotoxicology -Principles of testing of immunomodulating and immunotoxicological properties of drugs andXenobiotics

Unit – V 12 HrsDrug Pharmacology: Chemical transmission and drug action in the CNS. Diuretics, Drugs alteringthe pH of urine, excretion of organic molecules. Molecular Cardiology: Congenital Heart Disease,Inherited Cardiomyopathies, Coronary Atherosclerosis, Derived Nitric Oxide and Control of VascularTone, Hypertension, Cardiac Arrhythmias, Cardiovascular Gene Therapy. PULMONOLOGY:Asthma, Pulmonary Emphysema. Lung Cancer: The Role of Tumor Suppressor Genes – Strategiesfor controlling the diseases.Drugs acting on GIT : Antacids and anti-ulcer drugs, Laxatives and Anti-diarrhoeal drugs, Appetitestimulants and suppressants, Emetics and anti-emetics. Thyroid hormones and anti-thyroid drugs ACTH and corticosteroids, Androgens and anabolicsteroids, oral contraceptives. Treatment of poisoning, Heavy metals and heavy metal antagonists,Acute, Sub acute and Chronic toxicityExpected Course Outcomes:After going through this course the student will be able to:CO1: Discuss the multidisciplinary nature of biotechnology and apply specific knowledge to

pharmaceutical research and development. CO2. Evaluate current developments in pharmaceutical biotechnology and their applications through

appraisal of both philosophical and ethical issues.CO3 Apply knowledge/theory to new situations e.g. the formulation of hypotheses and experimental

design.CO4. Describe approved biotech products, e.g., indications, advantages, disease impact, & product

limits, & status of pipeline products, e.g., development issuesReference Books:

1 Pharmaceutical Biotechnology: Fundamentals and Applications by Daan J. A. Crommelin, RobertD. Sindelar, Bernd Meibohm. Springer Science & Business Media, 2013. ISBN: 1461464862, 9781461464860

2 Pharmaceutical Biotechnology Volume 655 of Advances in Experimental Medicine and Biology by Carlos A. Guzmán, Giora Z. Feuerstein, Springer Science & Business Media, 2010. ISBN: 1441911324, 9781441911322

3 Pharmaceutical Biotechnology: Drug Discovery and Clinical Applications by Oliver Kayser, Heribert Warzecha, John Wiley & Sons, 2012, ISBN: 352765125X, 9783527651252

4 Goodman and Gilman's Manual of Pharmacology and Therapeutics by Laurence L. Brunton, Randa Hilal-Dandan. McGraw Hill Professional, 2013. ISBN: 007176917X, 9780071769174



Scheme of Continuous Internal Evaluation (CIE):CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 markseach and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE(Theory) will be 100 marks.Scheme of Semester End Examination (SEE):The question paper will have FIVE questions with internal choice from each unit. Each question willcarry 20 marks. Student will have to answer one question from each unit. The total marks for SEE(Theory) will be 100 marks.Mapping of COs with POs and PSOs

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11CO1 M H H H H L L - M H MCO2 M M H L H L M L M H LCO3 H H M H M M M - M M HCO4 H H L L M M L H L M -

Mapping of CO with PSOPO1 PO2 PO3

CO1 H H HCO2 M H HCO3 H H MCO4 M M M



Agricultural BiotechnologyCourse Code : 16MBT242 CIE Marks : 100

Hrs/Week : L:T:P:S 4/0/0/0 SEE Marks : 100

Credits : 4 SEE Duration : 3 hrsCourse Learning Objectives (CLO):Graduates shall be able to1 Obtain a solid foundation in principles and fundamentals of plant and animal cell cultures and itsapplication.2. Understand the various breeding techniques for crop improvement. 3. Emphasize on potential applications of genetically engineered crops4. Understand various modes and mechanism for livestock improvement and ethical issues concern.5. Get an overview of the various applications of agri-biotechnology

Unit – I 9 HrsConcepts and scope of Agricultural biotechnology. Tissue culture in crop improvement,Micropropagation. Meristem culture and production of virus-free plants. Haploids in plant breeding;Anther and microspore culture. Embryo and ovary culture. Somatic hybridization; Protoplastisolation and fusion, cybrids. Somaclonal variation. Synthetic seeds. Cryopreservation, Productionof secondary metabolites, Elicitation with various biotic and abiotic elicitors.

Unit – II 10 HrsClassical and molecular plant breeding: Breeding methods for self and cross pollinated crops.Conventional methods for crop improvement (Heterosis breeding, Mutation breeding, ploidybreeding). Self incompatibility and male sterility in crop breeding for crop improvement. MolecularBreeding: – Molecular tagging of genes/traits. Marker-assisted selection of qualitative andquantitative traits, Screening and validation; Trait related markers and characterization of genesinvolved, Gene pyramiding, Transcript mapping techniques.

Unit – III 10 HrsGenetic engineering for crop improvement: Manipulation of Photosynthesis, Nitrogen fixation,Nutrient uptake efficiency. Molecular mechanisms of biotic stress resistance (Insects, fungi,bacteria, viruses, weeds) and abiotic stress tolerance (drought and salt) plants. Genetic engineeringfor quality improvement of Protein, lipids, carbohydrates, vitamins & mineral nutrients, Concept ofmap-based cloning and their application in transgenics.

Unit – IV 10 HrsAnimal Biotechnology: Fundamentals of animal cell culture. Classical and Molecular breeding inanimals, Marker assisted selection. Animal cloning; Transgenic animals, cloning of animals,Overview of Embryo Transfer in Farm Animals; Somatic Cell Nuclear Transfer and Other AssistedReproductive Technologies. Basic principles for the production of transgenic fish, poultry breeds.Biosafety: Introduction to Biological Safety cabinets. Biosafety guidelines and Regulatoryframeworks in India, GMOs & LMOs; Roles of Institutional Biosafety Committee, RCGM, GEACetc. for GMO applications in agriculture.

Unit – V 9 Hrs



Commercialization of agricultural biotechnology: Green food production, Green house technologyand protected cultivation: Types of Green house, Various component of green house, Design, criteriaand calculation. Green house irrigation system, Pytotrons: Hydroponics and aeroponics. OrganicFarming: Concept of Integrated nutrient management and Integrated pest management, molecularfarming in animals and plants.Expected Course Outcomes:After going through this course students will be able to:CO1:Remember and explain various fundamentals of Agricultural Biotechnology with reference to breeding techniques and regulatory frameworksCO2: Apply the knowledge of modern tools to analyze the improvement of agricultural practices and livestocks CO3: Evaluate and analyze various parameters of transgenics for crop and livestock improvementCO4: Create paraphernalia for better usage and production of agri based productsReference Books:1. S S Purohit, Agricultural Biotechnology, Agribios India, 2nd ed. 2003, digitalized 2011,

ISBN:81-7754-156-0.2. Stuart J. Smyth, Peter W.B. Phillips and David Castle, Handbook on Agriculture,

Biotechnology and Development, Edward Elgar Publications, 1st ed,2015 ISBN: 9781783471355.

3. Adrian Slater, Nigel Scott and Mark Fowler, Plant Biotechnology-The genetic manipulation ofplants, Oxford university press, 2nd ed, 2010, ISBN-13:9780199282616.

4. Maarten J. Chrispeels and David E. Sadava , Plants, Genes, And Crop Biotechnology, Jones andBartlett Publishers, 2nd ed. 2003, ISBN-13: 978-0763715861.

Scheme of Continuous Internal Evaluation (CIE):CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 markseach and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE(Theory) will be 100 marks.

Scheme of Semester End Examination (SEE):The question paper will have FIVE questions with internal choice from each unit. Each question willcarry 20 marks. Student will have to answer one question from each unit. The total marks for SEE(Theory) will be 100 marks.

Mapping of COs with POsPO1 PO

2PO3

PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 H H M L H L M - M M LCO2 H H M M M L H - H H LCO3 M M M M M L H - M H LCO4 H H H M H L H - M H H


CO1 L M H

CO2 H H HCO3 H M H



CO4 H H H

Recombinant DNA products




1. Understand the principle and techniques of therapeutic protein production in microbes.2. Study the techniques involved in production of vaccines in microbes. 3. Know the importance of recombinant DNA technology for production of important products in

microbial system. 4. Acquire advanced knowledge on molecular farming in plants. 5. Understand the techniques required for animal cell manipulation for production of important products.

Unit – I 10 Hrs

Therapeutic agents in Microbes: Protein therapeutics: Pharmaceuticals (Human interferons, humangrowth hormone, Tumdour Necrosis factor Alpha), Enzymes (Alginate Lyase, PhenylalanineAmmonia Lyase), HIV inhibitor, Recombinant antibodies (Human monoclonal antibodies, anticancerantibodies). Nucleic acids as therapeutic agents: Antisense RNA/Oligonucleotides, Ribozymes,Aptamers, Interfering RNAs.

Unit – II 9 Hrs

Vaccines in microbes: Vaccines: Subunit Vaccines (Herpes Simplex Virus, Foot-and-Mouth Disease,Cholera, SARs, Staphylococcus aureus, Human Papillomavirus), Peptide vaccines (Foot-and-MouthDisease), DNA vaccines, Attenuated Vaccines (Cholera, Salmonella species), Vector vaccines(Vaccinia virus, Mycobacterium tuberculosis)

Unit – III 9 HrsCommercial products in microbes: Restriction endonucleases, Lipase, Amino acids (L-Glutamicacid), Antibiotics (Undecylprodigiosin, 7-aminodeacetoxycephalospopranic acid), Biopolymers(Xanthan gum, Melanin, Polyhydroxyalkanoates, Hyaluronic acid), Microbial insecticides (Cryproteins, Chitinase), Isopropanol, Cellulase, Recombinant spider silk protein.

Unit – IV 10 HrsHeterologous proteins in Plants: Amino acids (methionine), Lipids (omega-3 and omega-6 fattyacids), Vitamins (Vitamin E, β-Carotene), Iron, Starch (α-amylase and glucose isomerase in potato),Flower pigmentation, Antibodies (IgG antibodies), Edible Vaccines (Cholera),

Unit – V 9 HrsHeterologous proteins in Animals: Production of biopharmaceuticals from Milk [Cystic fibrosistransmembrane regulator (CFTR)], Immunotherapeutic Potential of Antibodies Produced in ChickenEggs, Increased muscle mass in Transgenic mice, High levels of omega-6-fatty acids, improvinggrowth rate (Fish),



Expected Course Outcomes:

After going through this course the student will be able to:CO1: Explain the techniques involved in heterologous protein production in microbes, plant and

animal cells.CO2: Apply techniques required for recombinant DNA technology for production of compounds in

prokaryotes and eukaryotes. CO3: Compare and contrast between therapeutic proteins, vaccines between recombinant product

developed from microbes, plants and animals.CO4: Develop methodology for production of heterologous proteins in microbes, plants and animals. Reference Books:

1. Glick BR, Pasternak JJ, and Patten CL, Molecular Biotechnology – Principles and applicationsof recombinant DNA, ASM Press, 4th Edition. 2010. ISBN:978-1-55581-498-4.

2. Brown T.A. Gene Cloning and DNA Analysis – An Introduction, Wiley-Blackwell Science, 6th

Edition, 2010, ISBN: 9781405181730.3. Baltz RH, Demain AL, Davies JE (Edis), Manual of Industrial Microbiology and Biotechnology,

ASM Press; 3 edition, 2010 ISBN-13: 978-1555815127.

4. Clark DP, Biotechnology, Academic Cell, 2 edition, 2015, ISBN-13: 978-0123850157.5. Wagner R, Hauser H, Animal Cell Biotechnology, De Gruyter,1st edition, 2014, ISBN-13: 978-

3110278866. Scheme of Continuous Internal Evaluation (CIE):CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 markseach and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE(Theory) will be 100 marks.Scheme of Semester End Examination (SEE):The question paper will have FIVE questions with internal choice from each unit. Each question willcarry 20 marks. Student will have to answer one question from each unit. The total marks for SEE(Theory) will be 100 marks.Mapping of COs with POs

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 L L L L L L M MCO2 H M M M M L M MCO3 H M M M M L M MCO$ H M M M M L M M

Mapping of Cos with POsPSO1 PSO2 PSO3

CO1 L L LCO2 H M MCO3 L L LCO4 H H H


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https://www.amazon.com/s/ref=dp_byline_sr_book_1?ie=UTF8&text=Roland+Wagner&search-alias=books&field-author=Roland+Wagner&sort=relevancerank

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BIOREACTOR ENGINEERING



Credits : 4 SEE Duration : 3 hrsCourse Learning Objectives (CLO):Graduates shall be able to1. Learn the basics of rate law and analysis of rate data of homogeneous reactions 2. Understand about the ideal reactors and their function3. Understand the basics of nonideal flow and residence time distribution function4. Understand the microbial growth kinetics5. Learn about internal and external mass transfer effects and methods of minimizing the

resistances for a heterogeneous systemUnit – I 8 Hrs

Homogeneous reactions: Definition of reaction rate, temperature dependency from Arrhenius law,integral and differential method of analysis of rate equation, general reaction kinetics for biologicalsystems: zero, first and second order irreversible reactions (final integral rate equation, noderivation), numericals.

Unit – II 12HrsIdeal reactor operation:Batch reactor operation with Michaelis-Menten kinetics, cell culture inbatch reactor, fed batch operation of mixed reactor, Mixed reactor operation with Michaelis-Menten kinetics,Continuous cell culture: Optimum dilution rate and critical dilution rate in IdealChemostat, chemostat with immobilized cells, chemostat cascade, Plug flow reactor withMichaelis-Menten kinetics, comparison between major modes of reactor operation, numericals.

Unit – III 10HrsNon Ideal Flow: Interpretation of RTD curve: C, E and F curves, step and impulse input responsefor the non ideal reactors. Exit age distribution of fluid in reactors, RTD’s for CSTR and PFR,calculation of conversion for first order reaction. Tank in series model.

Unit – IV 8HrsGrowth Kinetics of Microorganisms: Transient growth kinetics, Substrate limited growth,Models with growth inhibitors, Logistic equation, Filamentous cell growth model. Growthassociated and non growth associated product formation kinetics, Leudeking – Piret models,substrate and product inhibition on cell growth and product formation

Unit – V 10HrsHeterogeneous system: Interaction between mass transfer and reaction, concentration gradientsand reaction rates in solid catalysts, steady state shell balance, thiele modulus and effectivenessfactor, concentration profiles for spherical geometry and flat geometries, internal and external masstransfer effects, minimizing internal and external mass transfer effects

Course Outcomes:

1) Understand the basic concept of application of rate law to homogeneous system2) Apply the design equation of ideal reactors to problems of single reactions



3) Understand various microbial growth and inhibition models and their applications

4) Evaluate the rate equation for catalytic reactions and determine the parameters of rate expression.

REFERENCE BOOKS:1) P.M. Doran; Bioprocess Engineering Principles; Academic Press; 2nded; 2012;

ISBN:9780122208512) M.Shuler and F. Kargi; Bioprocess Engineering; Prentice Hall; 2nd ed; 2002;

ISBN:01308190853) Octave Levenspiel, Chemical Reaction Engineering, John Wiley and Sons, 3rd ed. 19994) H.S Fogler; Elements of Chemical Reaction Engineering; Prentice Hall; 4th ed; 2006;

ISBN:01304739445) T.Panda, Bioreactors: analysis and design, Tata McGraw Hill education Pvt Ltd, New

Delhi, 1st ed. 2011

Scheme of Continuous Internal Evaluation (CIE):CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 marks each and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE(Theory) will be 100 marks.Scheme of Semester End Examination (SEE):The question paper will have FIVE questions with internal choice from each unit. Each question will carry 20 marks. Student will have to answer one question from each unit. The total marks for SEE (Theory) will be 100 marks.Scheme of Continuous Internal Evaluation (CIE) for Lab:

Scheme of Semester End Evaluation (SEE) for Lab:

Mapping of COs with POsPO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 H M M M L L -- -- L -- LCO2 M H H M L L -- -- L -- LCO3 H L M L L L -- -- L -- LCO4 H M M M L L -- -- L -- L


CO1 M M LCO2 M H LCO3 M M LCO4 M M L


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