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!"#$%&'#()*+,--%.%*/!0-#"***!***/!0-#"*+#()*!"#$%&'#()***!***()"/1--*"1(#,"1-*#"'(#(!(%** 2*
Real-time Sweat Analysis: Concept and Development of an Autonomous Wearable
Micro-fluidic Platform
Vincenzo F. Curto
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OUTLINE
• Sweat, why is it Important?
• Introduction: Wearable Sensors
• Fabrication and Characterisation of the Devices
• Results
• Conclusions
3*
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Sweat is naturally generated during exercise.
Monitoring its contents provides very rich information about the physiological condition of the individual.
SWEAT, WHY IS IT IMPORTANT?
*Common hereditary disease which affects the entire body, causing progressive disability and often early death.
Sweat analysis: identify pathological disorders " Cystic fibrosis* " Information on dehydration " Changes in the concentration of biomolecules and ions " Hyponatremia (low sodium concentration)
Rehydration and re-mineralisation
Improve performance and general health
4*
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LIFESHIRT® !"#$%&''($)i'*+)),-./01)#203)
PHYSIOLOGICAL & CHEMICAL SENSORS
# Breath rate, # Heart rate, # Posture # Skin temperature # …
Medtronic Diabetes’ Guardian®
Lactate Scout®
5*
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SWEAT ANALYSIS
PharmChek Sweat Patch
Macroduct sweat collection system
S. M. Shirreffs and R. J. Maughan, J Appl Physiol 82: 336-341, 1997
Whole body sweat collection techniques
6*
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WHAT DO WE NEED????
DEVICE: WEARABLE
ROBUST
FLEXIBLE / ADAPTABLE
REUSABLE/ DISPOSABLE (CHEAP)
IMMEDIATE FEEDBACK
REAL-TIME SWEAT ANALYSIS
DETECTION:
NOT INVASIVE
WIRELESS
FREEDOM FROM ELECTRONIC NOISE
MINIATURIZATION
FLEXIBILITY IN INTERROGATION APPROACHES (human eyes, LED-sensors, cameras, spectrometers, …)
7*
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BIOTEX)4)Biosensing Textile for Health Managment
S. Coyle, IEEE Transactions on Information Technology In Biomedicine, VOL. 14, No. 2, MARCH 2010
Sodium Sensor
Conductivity Sensor
Temperature Sensor
www.biotex-eu.com
55 mm
40 mm
8*
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DEVELOPMENT OF THE CONCEPT
9*
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OPTICAL DETECTION
Yellow LED 590 nm
:*
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smLED
photodiode
# Microcontroller # Battery Pack
antenna
DETECTORS Wireless detector
smLED
photodiode
radio receiver
# Microcontroller # Battery Pack # Transmitter
Wired detector smLED photodiode
Microcontroller
Portable detector
2;*
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MICRO-DEVICE: Fabrication
Main Characteristics:
- pH dye bromocresol purple
- Absorbent = passive pump
absorbent
22*
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Loading capacity of the absorbent
pKa determination of Bromocresol purple in the chip
Absorbent
MICRO-DEVICE: Characterisation
23*
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Arduino Micro-controller
PERFORMANCE
24*
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Stability of the signal after 50 min. exercise. LED alignment is not perturbed with athlete movement
LED-PHOTODIODE STABILITY
Reference Measured
pH
Sweat reaches the Sensing area
Activated pH 4 solution
25*
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Main Characteristics:
- pH dye bromocresol purple
- Flow = controlled by the thread
- Absorbant= passive pump
absorbent thread
Inlet
Channel
Sensing area
Absorbant
26*
MICRO-DEVICE: Fabrication
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Acidic pH = 1 [H+]=0.1 M
Basic pH = 12 [OH-]=0.01M
27*
MICRO-DEVICE:)Characterisation
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3 4 1 2 Flow
Microchip loading absorbant capacity (n=5)
68.7±6 µl
LIFE-TIME: ~ 63 MINUTES
MICRO-DEVICE: Characterisation
28*
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ON BODY TRIAL
Resting time (easy cycling)
pH electrode Measured
Warm up
29*
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• The fabrication, characterisation and the performance of a wearable micro-fluidic system based on a textile fabric for monitoring in real time mode the pH of the sweat generated during an exercise period has been presented.
• Minituarisation of the detector system
• Durability and accuracy of the device during athlete performance.
• Immediate feedback regarding sweat composition, pH, to individuals during sport activities.
• Run on body trials using the fully Autonomous Cordless platform by Tyndall.
• Establishment of a correlation between pH of the sweat and other physiological parameters, such as pH and lactate of blood and sweat electrolytes.
2:*
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Dr. Shirley Coyle Dr. Fernando Benito Lopez
Ms. Sarah Hughes
Prof. Niall Moyna
Prof. Dermot Diamond
07/CE/I1147
Dr. Robert Byrne
Mr. Javier Torres Eng. Philip Angove
Research Career Start Programme 2010
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QUESTIONS?