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Removal of heavy metals and phosphates from wastewater by bioparticles . The 2 nd International Symposium “Vera Johanides ” BIOTECHNOLOGY IN CROATIA BY 2020. Lucija Nuskern , mag . oecol . Jasna Hrenović , PhD. Zagreb, 10 – 11 May , 2013. 1. Introduction. - PowerPoint PPT Presentation
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Removal of heavy metals and phosphates from
wastewater by bioparticles.
The 2nd International Symposium “Vera Johanides” BIOTECHNOLOGY IN CROATIA BY 2020
Zagreb, 10 – 11 May, 2013
Lucija Nuskern, mag. oecol.Jasna Hrenović, PhD
1. IntroductionNeed for wastewater treatment prior to
release into receiving environment◦Heavy metals – toxic activity, bioacumulation◦Phosphates – eutrophication
BIOPARTICLE – particle composed of inorganic carrier and biofilm of bacteria◦higher density of bacterial population,
bacteria protected from biotic and abiotic stresses, no bacterial wash-off better treatment efficiency
Aim of the studyDesign bioparticles able to:
1) tolerate exposure to high concentrations of Cu2+, Zn2+ and Ni2+
2) Simultaneously remove those heavy metals and phosphates (PO4
3-) from wastewater
2. Materials and methodsNatural zeolitic tuff (Vranjska Banja,
Srbija) – carrier material
Bacteria: Acinetobacter junii DSM 1531 ◦ P - accumulationPseudomonas aeruginosa DSM 15980◦ Heavy metal tolerance
Mineral composition (wt %): clinoptilolite (72.6), plagioclase (14.6), quartz (12.8)
Particle size: 0.063 – 0.121 mm
Bacterial immobilisationIncubation of zeolitic tuff with
pure bacterial culture of A. junii or P. aeruginosa in liquid medium, 24 h/30 °C with aeration
Wet bioparticles with immobilised A. junii
(AJBP)
Wet bioparticles with immobilised P.
aeruginosa
(PABP)
Experimental procedure1. Planctonic bacteria
2. Immobilised bacteria (bioparticles - BP)
1 mLA. junii or
P. aeruginosa
C1 – C5:1, 5, 10, 25 and 50 mg Cu2+/L1, 5, 10, 35 and 70 mg Zn2+/L1, 5, 10, 25 and 50 mg Ni2+/L
100 mL P -
medium+
1 g wet AJBP or PABP
100 mL P -
medium+
C1, C3 and C5:1, 10 and 50 mg Cu2+/L1, 10 and 70 mg Zn2+/L1, 10 and 50 mg Ni2+/L
Experimental procedure 3. Mixed AJBP and PABP in synthetic wastewater
4. Mixed AJBP and PABP in real wastewater
0.5 g wet AJBP and0.5 g wet
PABP
100 mL P -
medium+
0.5 g wet AJBP and0.5 g wet
PABP
100 mL efluent +
C1, C3 and C5:1, 10 and 50 mg Cu2+/L1, 10 and 70 mg Zn2+/L1, 10 and 50 mg Ni2+/L
C1, C3 and C5:1, 10 and 50 mg Cu2+/L1, 10 and 70 mg Zn2+/L1, 10 and 50 mg Ni2+/L
3. RESULTS - Heavy metal tolerance PLANCTONIC vs. IMMOBILISED bacteria
Control C1 C3 C50.02.04.06.08.0
10.0Planctonic Immobilised
c (Cu2+)/ mg dm-3
log
CFU
Control C1 C3 C50.02.04.06.08.0
10.0
c (Zn2+)/ mg dm-3
log
CFU
Control C1 C3 C50.02.04.06.08.0
10.0
c (Ni2+)/ mg dm-3
log
CFU
A. junii
Control C1 C3 C50.02.04.06.08.0
10.0Planctonic Immobilised
c (Cu2+)/ mg dm-3
log
CFU
P. aeruginosa
Control C1 C3 C50.02.04.06.08.0
10.0
c (Zn2+)/ mg dm-3
log
CFU
Control C1 C3 C50.02.04.06.08.0
10.0
c (Ni2+)/ mg dm-3
log
CFU
3. RESULTS - Heavy metal removal PLANCTONIC vs. IMMOBILISED
bacteria
Highest heavy metal removal was achieved with PABP: 84,1 % Cu2+, 100 % Zn2+ and 83,7 % Ni2+
C1 C3 C50.020.040.060.080.0
100.0Cu, PABP % Cu removed planctonic
% Cu removed immobilised
% C
u2+
rem
oval
C1 C3 C50.020.040.060.080.0
100.0Zn, PABP % Zn removed planctonic
% Z
n2+
rem
oval
C1 C3 C50.020.040.060.080.0
100.0Ni, PABP % Ni removed planctonic
% Ni removed immobilised%
Ni2
+ r
emov
al
3. RESULTSMixed AJBP and PABP in real wastewater
AJBP and PABP survived, multiplied and removed heavy metals and phosphates when in the same system
Control C1 C3 C50.0
20.040.060.080.0
100.0
Cu
P in P-medium P in efluentCu in P-medium Cu in efluent
% r
emov
ed
Control C1 C3 C50.0
20.040.060.080.0
100.0Zn
P in P-medium P in efluentZn in P-medium Zn in efluent
% r
emov
ed
Mixed AJBP and PABP removed heavy metals and phosphates from real wastewater more efficiently than from synthetic wastewater
4. CONCLUSION
Bioparticles composed of immobilised bacteria A. junii and P. aeruginosa on natural zeolitic tuff could be used to simultaneously remove heavy metals and phosphates from wastewater
THANK YOU FOR YOUR ATTENTION!