Immunostimulatory effect of Recombinant Antimicrobial Peptides supplemented feed on Megalobrama amblycephala against Aeromonas hydrophila

Requirements： 1. 300 M.amblycephala fish 400 – 500g weight

2. Aquarium:

a) To acclimatize healthy and diseased fish

3. Dissection set

4. cDNA synthesis kit

5. Antimicrobial peptide cecropin http://item.taobao.com/item.htm?

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6. SYBR Green

7. Trizol reagent

8. EDTA

9. Primers

10. SOD, Lysozyme activity and respiratory burst assay kit

Feed Ingradients:

1. Fish Meal

2. Squid Meal

3. Starch

4. Soybean meal

5. Mineral mixture

6. Vitamin mixture

7. Fish oil