REVIEW I: TRANSMISSION GENETICS

exam 1: W.10.11 chapters 1 – 5

1. genetics & the organism2. patterns of inheritance3. chromosomal basis of inheritance4. eukaryotic chromosome mapping

by recombination5. genetics of bacteria & their viruses

basic concepts genetic material does what 3 things ? patterns of transmission + meiosis = ? what are genes & what are they doing ?

variation continuous & discontinuous P = G + E + G*E norms of reaction

CH01: GENETICS & THE ORGANISM

G1

G2

PHENOTYPE

G

ENVIRONMENT

E1

E2

E1

E2

G+E

E

E1

E2

E1

E2

G*E

NORMS OF REACTION

quantitative methods artificial selection

classical methods mutagenesis, selection screens map, function, molecular biology

complications expressivity, penetrance polygeny, pleiotropy

what is a good model organism ?

CH01: GENETICS & THE ORGANISM

xx0.05

0.10

0.15

0.20

0.25

0.30

0.35

FREQUENCY

0.00

PHENOTYPE

GENETIC COMPONENT

key terms on p. 24 no assigned questions

CH01: GENETICS & THE ORGANISM

basic concepts what evidence for the existence of genes ? single genes & discrete phenotypes what are Mendel’s laws ? how are sex-linked genes inherited ?

Mendelian analysis controlled breeding & terminology 1 gene: monohybrid & test cross ratios 2 genes: dihybrid & test cross ratios

CH02: PATTERNS OF INHERITANCE

Mendelian analysis… continued methods: Punnet, long & short trees formulae to predict outcomes hypothesis testing with 2 binomial expansion to predict outcomes Poisson distribution to predict outcomes

sex-linked inheritance pedigree analysis

rules & applications

CH02: PATTERNS OF INHERITANCE

key terms on p. 59 all questions beginning on p. 62

CH02: PATTERNS OF INHERITANCE

basic concepts how do we know genes are on chromosomes ? how are genes arranged on chromosomes ? are chromosomes only genes ? how is chromosome number maintained ? what are the chromosomal bases of Mendel’s laws?

chromosome theory of inheritance evidence supporting theory

chromosome structure & organization

CH03: CHROMOSOMAL BASIS OF INHERITANCE

mitosis & meiosis stages & chromosome movement differences & similarities of processes relationships to gene transmission fungal genetics introduced

CH03: CHROMOSOMAL BASIS OF INHERITANCE

CHROMOSOMES & INHERITANCE

key terms on p. 107 questions 1, 2, 5-10, 12, 16, 18-20, 22,

25-30, 32, 33, 40 beginning on p. 109

CH03: CHROMOSOMAL BASIS OF INHERITANCE

basic concepts genes mapped by recombination frequencies distances are additive one recombination event can interfere with another

linkage linked vs unlinked inheritance with 2 test for linkage

linkage maps 3 point test cross

CH04: CHROMOSOME MAPPING & RECOMBINATION

linkage maps (continued) accuracy in mapping by recombination mapping function based on Poisson disctribution single meioses in fungi

ordered analysis unordered analysis

CH04: CHROMOSOME MAPPING & RECOMBINATION

key terms on p. 137-8 questions 1-30 beginning on p. 141

CH04: CHROMOSOME MAPPING & RECOMBINATION

basic concepts how can bacterial genomes recombine ? how can viral genomes recombine ? how do bacterial & viral genomes interact ? how can we map genomes of these organisms ?

working with microorganisms growing them mutants

CH05: GENETICS OF BACTERIA & THEIR VIRUSES

conjugation in bacteria first evidence, F plasmids F-, F+ & Hfr strains interrupted mating experiments… gene order high resolution mapping F’ plasmids

transformation in bacteria co-transformation mapping

CH05: GENETICS OF BACTERIA & THEIR VIRUSES

phage genetics phage cross recombination in phage

transduction in bacteria life cycles generalized transduction, lytic cycle specialized transduction, lysogenic cycle co-transduction mapping

CH05: GENETICS OF BACTERIA & THEIR VIRUSES

key terms on p. 176-7 questions 1-10, 12-32

beginning on p. 179

CH05: GENETICS OF BACTERIA & THEIR VIRUSES