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RM-8 Respirometry Flow Multiplexer
S A B L E E N A B L E S
Running experiments in parallel rather than in series greatly increases throughput and makes better use of time and equipment. The RM-8 respirometer multiplexer allows you to select from any one of seven respirometer sample streams with full baselining available at any time on the eighth channel.
Each stream consists of a pair or precision, fast-operating electrical valves. When activated, these valves connect the selected stream to a pair of common ports. The RM-8 can switch between streams and baselines manually or automatically, between or during recordings with appropriate
software, such as ExpeData. Manual channel selection, or user-programmed channel selection (up to 99 steps), or daisy-chainable serial (RS-232) channel selection can also
be selected. Multiple instruments can be merged to create almost any number of desired sample streams.
What happens to respirometer chambers that are not selected?
The RM-8 allows respirometer chambers that are not selected to be flushed continuously with a user-provided airstream to prevent hypercapnic
Automated gas sampling from multi-sample and multi-animal respirometry.
F EATURES
Selects from up to eight air streams for gas analysis
Multiplexes electrical signals
Perfect for multi-animal flow-through or stop-flow respirometry
Easy interface to almost any data acquisition system
Can be operated manually or in stand-alone program mode
C L A S S I C L I N E
or hypoxic conditions from developing between readings. Alternatively, the FlowBar-8 mass flow generator can be used to control air-flow independently through all chambers at once, and the RM-8 can then be used to sub-sample the air emerging from each chamber, allowing very fast switching from chamber to chamber. The RM-8 can deliberately prevent any gas flow while the chamber is not selected, thus transforming itself into a stop-flow multiplexer. The stop-flow configuration prevents cross-contamination between samples.
RM-8 Respirometry Flow Multiplexer
Sable Systems International3840 N. Commerce StreetNorth Las Vegas, NV 89032, USATELEPHONE:US: +1 800 330 0465 / + 1 702 269 4445EMAIL: [email protected]
Sable Systems Europe GmbHOstendstr. 25D-12459 Berlin, GermanyTELEPHONE: +49 30 5304 1002MOBILE: +49 176 2078 7008FAX: +49 30 5304 1003
12/2018
www.sablesys.com
Example of Stop-flow Multiplexing on n=8 chambers each containing n=10 Drosophila (Flow rates=100ml/min). Baselines are conducted every 10 minutes. Note that the integral of each peak is directly proportional to metabolic rates.
Example of Flow-through Multiplexing on n=4 laboratory mice (flow rates=2 LPM). Baselines are conducted every 2.5 minutes. Note that a dwell time of only 30 seconds is sufficient to determine metabolic rates.
Optional Configurations
NEW “Small volume” configuration includes 1/16” internal tubing to minimize dead space and maximize response times when low flow rates (<100ml/min) are desired or in some stop-flow configurations.
Optional MuxScan II – Electrical Multiplexer
By using this adapter, you can quickly and easily connect up to 8 activity detectors or other transducers (range: 0 to 5V) to your RM-8, and the adapter will select the appropriate detector automatically when its corresponding solenoid valve is energized. All connections are made via research-grade BNC connectors, except for the connection to
the multiplexer itself, which is accomplished via a DB-15 connector with attached cable (supplied). The multi-activity adapter also has provision for biasing resistive
detectors such as thermistors, light dependent resistors (handy for activity detection at moderate light levels) and phototransistors using either visible light or invisible infrared illumination.
The RM-8 can be configured for flow-through or stop-flow respirometry as demonstrated in the following graphs.
SPEC I F I CAT I ONS
RM-8CHANNELS Eight; two valves per channel
FLOW RATE RANGE 0 – 500 ml/min recommended; commonly used at 150 – 200 ml/min, push or pull mode
OPERATION Stop flow or flow-through compatible
AUTOMATIONCan switch between inputs unattended, in any sequence, for selectable durations, in single-shot or looping mode
OPERATING TEMPERATURE 5 – 45˚ C, relative humidity non-condensing
DISPLAY Digital 2 x 16 alphanumeric LCD
POWER REQUIREMENTS 12 – 15VDC; universal adapter supplied
RESPONSE TIME < 50 milliseconds for complete valve changeover
DIMENSIONS (W x D x H) 8 x 12 x 6 in. (20.3 x 30.5 x 15.2 cm)
WEIGHT 8 lbs. (3.6 kg)
TECHNOLOGY Electrically activated three-way solenoid valves
CONNECTIONS Nickel plated barb fittings for 1/8 in (3 mm) i.d. flexible or semi-rigid tubing
Optional MuxScan – Electrical MultiplexerANALOG INPUT CHANNELS 8 BNC
ANALOG INPUT RANGE 0 – 5V
ANALOG OUTPUT CHANNELS 1 BNC
OPERATING TEMPERATURE 5 – 45˚ C relative humidity non-condensing
POWER REQUIREMENTS 12 – 15 VDC; universal adapter supplied
DIMENSIONS (W x D x H) 5 x 3 x 2 in. (12.7 x 7.6 x 5.1 cm)
WEIGHT 1 lb (0.5 kg)
0
200
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800
1,000
1,200
1,400
1,600
0 1 2 3 4 5Time (min)
CO
(ppm
)2
O (%
)2
20.70
20.75
20.80
20.85
20.90
20.95
21.002
Baseline
3 4 5Chamber # Baseline Baseline
2 3 4 5Chamber #
0
50
100
150
200
250
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450
0 1 2 3 4 5 6 7 8 9 10Time (minutes)
CO
(ppm
)
-0.004
0.000
0.004
0.008
0.012
0.016
0.020
O Change (%
)2
2
2Baseline 3 4 5 6 7 8Chamber #
