RNA Processing

RNA, ribonucleic acid

single strand polymers of ribonucleotides

have secondary and tertiary structure

Classification of RNATypes function

ribosomal RNA (rRNA)messenger RNA (mRNA)transfer RNA (tRNA)heterogeneous nuclear RNA(hnRNA)small nuclear RNA(snRNA)

small nucleolar RNA (snoRNA)small cytoplasmic RNAribozymemicroRNA (miRNA)

small interfering RNA (siRNA)non-coding RNA (ncRNA)

component of ribosometemplate of protein synthesistransfer amino acidpremature mRNA

participate the splicing and transfer of hnRNA, includes spliceosomal RNAprocess and modify rRNA

recognize the protein sorting signalcatalytic RNAusually endogenous, induce degradation of targeted mRNA usually exogenous, induce degradation of targeted mRNAall RNA other than mRNA, priorly functional RNA longer than 200nt

mRNA

Processing of mRNA Structure of mRNA

hnRNAm7Gppp

AAA….An

5’ 3’AUGGUG……………………………UAA5’-cap 5’ UTR coding region 3’ UTR 3’ poly(A) tail

triplet code or codon

mRNA(cont.)

The process of mRNA is on the base of hnRNP 5’-cap and 3’ poly(A) play a role in the translocation

of mRNA (from nuclear to cytoplasm), stability, control of protein translation.

Life Cycle of an mRNA

RNA interference and siRNA