Safe 2020 and Maxisafe 2020 BSC - Thermo Fisher Scientific.pdfValid: 01.2009 / 50118069 Operating Instructions Safety Cabinets Safe 2020 Maxisafe 2020

Valid: 01.2009 / 50118069

Operating InstructionsSafety Cabinets

Safe 2020Maxisafe 2020

Operating Instructions Safety Cabinet Safe 2020 / Maxisafe 2020

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© 2009 Thermo Fisher Scientific Inc. All rights reserved.These operating instructions are protected by copyright. Rights resulting the-reof, particularly reprint, photomechanical or digital postprocessing or reproduction,even in part, are only allowed with the written consent of Thermo Electron LED GmbH.This regulation does not apply to reproductions for in-plant use.

TrademarksAll trademarks mentioned in the operating instructions are the exclusive propertyof the respective manufacturers.

Thermo Electron LED GmbHRobert-Bosch-Straße 1D - 63505 LangenselboldGermany


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Contents

1. General notes ......................................................................................... 61.1 General safety instructions ............................................................. 61.2 Warranty ......................................................................................... 71.3 Explanation of symbols................................................................... 7

1.3.1 Symbols used in the operating instructions ............................. 71.3.2 Symbols on the device ............................................................. 8

1.4 Use of the device ............................................................................ 81.4.1 Correct use ............................................................................... 81.4.2 Incorrect use: ............................................................................ 9

1.5 Standards and safety regulations ................................................... 9

2. Delivery ................................................................................................. 102.1 Standard components ................................................................... 102.2 Acceptance inspection .................................................................. 102.3 Transport lock and device packaging ........................................... 10

3. Installation ............................................................................................ 113.1 Ambient conditions ....................................................................... 113.2 Room ventilation ........................................................................... 113.3 Correct location............................................................................. 123.4 Installation in line .......................................................................... 123.5 Transport ....................................................................................... 13

4. Unit description .................................................................................... 144.1 Overall view .................................................................................. 144.2 Safety system ............................................................................... 174.3 Filter system ................................................................................. 184.4 Controls and display ..................................................................... 204.5 Sample chamber access .............................................................. 214.6 Device interfaces .......................................................................... 224.7 Sample chamber illumination ....................................................... 234.8 UV lamp unit ................................................................................. 244.9 Working area................................................................................. 24

5. Start-up .................................................................................................. 255.1 Initial operation ............................................................................. 255.2 Installing unit and accessories ..................................................... 255.3 Levelling the cabinet ..................................................................... 275.4 Power supply connection ............................................................. 285.5 RS 232 interface connection ........................................................ 305.6 UV connection .............................................................................. 305.7 Installation test .............................................................................. 31

6. Handling and control ........................................................................... 326.1 Display .......................................................................................... 32

6.1.1 Functions of the display components ..................................... 326.1.2 Display during the calibration routine ..................................... 336.1.3 Display in OFF mode .............................................................. 336.1.4 Display in work mode ............................................................. 336.1.5 Power interruption .................................................................. 336.1.6 Display and functions after a power failure ............................ 346.1.7 Failure messages ................................................................... 34

6.2 Description of the operating modes ............................................. 356.3 Operation system ......................................................................... 37

6.3.1 Basic functions ....................................................................... 376.3.2 Moving the front window to the working position ................... 396.3.3 Silencing the audible alarm signal ......................................... 396.3.4 Switching the illumination on and off ...................................... 40


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Contents

6.3.5 Activating and deactivating the internal power supply ........... 406.3.6 Displaying the UV disinfection time........................................ 406.3.7 Activating and deactivating the potential-free contact

(optional) ................................................................................. 406.3.8 Switching the cabinet to OFF mode ....................................... 416.3.9 Setting the time....................................................................... 416.3.10 Displaying the downflow velocity ........................................... 426.3.11 Displaying the operating hours of the HEPA filters ................ 436.3.12 Displaying the Performance Factor ........................................ 436.3.13 Setting and activating the timer .............................................. 446.3.14 Deactivating the timer ............................................................. 456.3.15 Setting the UV disinfection time ............................................. 466.3.16 Starting the UV disinfection .................................................... 466.3.17 Cancelling the UV disinfection ............................................... 476.3.18 Activating the stop watch ....................................................... 47

6.4 Pilot switch .................................................................................... 486.4.1 Moving the front window......................................................... 486.4.2 Switching the device to OFF mode ........................................ 49

7. Operation .............................................................................................. 507.1 Hygiene preparations for the sample chamber ............................ 507.2 Preparing the sample chamber .................................................... 507.3 Response to failure messages ..................................................... 507.4 Work rules ..................................................................................... 51

8. Shut-down............................................................................................. 528.1 Interrupting an operation .............................................................. 528.2 Shutting the unit down .................................................................. 52

9. Cleaning and decontamination .......................................................... 539.1 Decontamination procedure ......................................................... 539.2 Wipe/spray disinfection................................................................. 539.3 UV disinfection after a wipe/spray disinfection ............................ 54

9.3.1 UV disinfection using the integral UV lamps .......................... 549.3.2 UV disinfection using a mobile UV device ............................. 559.3.3 Changing the UV disinfection time ............................................ 55

9.4 Sterilization with formaldehyde ..................................................... 569.5 Cleaning the exterior surfaces...................................................... 569.6 Cleaning the floorpan ................................................................... 56

10. Maintenance ......................................................................................... 5710.1 Inspection...................................................................................... 5710.2 Service .......................................................................................... 57

10.2.1 UV lamps ................................................................................ 5710.2.2 Sample chamber illumination ................................................. 5810.2.3 Replacing the front window seal ............................................ 58

10.3 Retrofitting and repairs ................................................................ 59

11. Disposal ................................................................................................ 6011.1 Disposal procedure ...................................................................... 60

12. Technical data ...................................................................................... 62

13. Device log ............................................................................................. 69

14. Certificate of decontamination ........................................................... 70


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Figures

Fig. 1 Device locations in a room ...........................................................12Fig. 2 Lift points ......................................................................................13Fig. 3-a Overall view / model Safe 2020 ...................................................15Fig. 3-b Overall view / model Maxisafe 2020 ............................................16Fig. 4-a Filter system with downflow filter and exhaust

air filter / model Safe 2020 ...........................................................18Fig. 4-b Filter system with primary filter, downflow filter and exhaust

air filter / model Maxisafe 2020 ....................................................19Fig. 5 Controls and indicators ................................................................20Fig. 6-a Access through front cover ..........................................................21Fig. 6-b Access through front window .......................................................21Fig. 7-a Supply interfaces ..........................................................................22Fig. 7-b Sample chamber illumination .......................................................23Fig. 8 UV lamp unit .................................................................................24Fig. 9 Working area on the workplate, armrests ....................................24Fig. 10-a Stand installation / model Safe 2020 ...........................................25Fig. 10-b Stand installation / model Maxisafe 2020 ....................................26Fig. 10-c Drain valve installation .................................................................27Fig. 11 RS 232 interface connection ........................................................30Fig. 12 UV connection ..............................................................................30Fig. 13 Indicator and operating panel ......................................................33Fig. 14 Indicator and operating panel ......................................................37Fig. 15 Basic functions of the pilot switch ................................................48Fig. 16 Sitting posture ..............................................................................52Fig. 17 Front window seal replacement ...................................................58


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1. General notes

The following are the addresses of the international ThermoSales Organisations.

1.1 General safety instructions

These safety instructions describe the safety features of the Safe 2020 andMaxisafe 2020 series.The safety cabinet has been manufactured in keeping with the latesttechnological developments and has been tested before delivery for its correctfunction. It may, however, present potential hazards if it is not used accordingto the intended purpose or outside of operating parameters. Therefore, thefollowing procedures must always be observed:• The safety cabinet must be operated only by trained and authorized personnel.• For any operation of this unit, the operator must prepare clear and concise

written instructions in the language of the operating and cleaning personnelbased on these operating instructions, applicable safety data sheets, planthygiene guidelines, and technical regulations, in particular:• which decontamination measures are to be applied for the cabinet and

accessories,• which protective measures apply while specific agents are used,• which measures are to be taken in the case of an accident.

• Repairs to the device must be carried out only by trained and authorized expertpersonnel.

• The contents of the operating instructions are subject to change withoutfurther notice.

• Concerning translations into foreign languages, the German version of theseoperating instructions is binding lease.

• Keep these operating instructions close to the unit so that safety instructionsand important information are always accessible.

• Should you encounter problems that are not detailed adequately in theseoperating instructions, please contact Thermo Electron LED GmbHimmediately for your own safety.

Postal address GermanyThermo Electron LED GmbHRobert-Bosch-Straße 1D - 63505 Langenselbold

Enquiries from Germany:PhoneSales 0800 1 536376Service 0800 1 112110FaxSales/Service 0800 1 [email protected]

International enquiriesTel. + 49 (0) 6184 / 90-6940Fax + 49 (0) 6184 / [email protected]


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1. General notes

1.2 Warranty

Thermo Electron LED GmbH warrant the operational safety and functions of thesafety cabinet only under the condition that:• the device is operated and serviced exclusively in accordance with its intended

purpose and as described in these operating instructions,• the device is not modified,• only original spare parts and accessories that have been approved by Thermo

Electron LED GmbH are used,• inspections and maintenance are performed at the specified intervals,• an installation test is performed prior to the initial operation of the device and

that a repeat test is performed on the occasion of all inspections and repairs.The warranty is valid from the date of delivery of the device to the operator.

1.3 Explanation of symbols

1.3.1 Symbols used in the operating instructions

WARNING!

is used if non-observance may cause seriousor even lethal injuries.

CAUTION!

is used if non-observance may cause mediumto minor injuries or damage.

NOTE

is used for hints and useful information.

RECYCLING!

Valuable raw materials can be reused.


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1. General notes

1.3.2 Symbols on the device

Observe operating instructions (switchbox ceiling)

Biohazard (left device front)

Cytostatic agents (left device front)

Checked safety (right side of device)

T5A note (sample chamber fusing)

RS 232 interface (connection label)

Armrest installation(right side of light dome)

1.4 Use of the device

1.4.1 Correct use

Model Safe 2020:The safety cabinet is a laboratory device for installation and operation inmicrobiological and biotechnical laboratories of safety levels 1, 2, and 3. It hasbeen designed as a Class II microbiological safety cabinet, in accordance withEN 12469 / 2000.Depending on the hazard level of the agents involved, the operator mustprepare in writing appropriate decontamination procedures for the device andthe accessories used in the sample chamber.


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1. General notes

Model Maxisafe 2020:The safety cabinet also meets the requirements from DIN 12980 / 2005-06 andcan be used for the production of cytostatic agents.For cytostatic agent applications, use the lowered one-piece working plate only.

Prior to the initial operation of the cabinet, the operator must perform aninstallation test. The test result must be documented by a test report. Thecabinet must only be released for operation if it is in compliance with theoperating parameters specified by Thermo Electron LED GmbH.

After any changes to the installation conditions or after any modification to thetechnical system, a repeat test must be performed and the test result must bedocumented by a test report that shows that all operating parameters are incompliance with those specified by Thermo Electron LED GmbH.

1.4.2 Incorrect use:The safety cabinet must not be used in laboratories that do not comply with therequirements of safety levels 1, 2, or 3.The unit must not be operated as a Class II safety cabinet, if:• no repeat test is performed after changes to the installation conditions or after

modifications to the technical system,• the alarm system of the device has issued a failure message and thecause for the failure has not been repaired.

The alarm system must not be tampered with or disabled. If alarm systemcomponents heve been removed or disabled for service or repairs, the unit mustonly be released for operation if all alarm system components are functioningagain properly.

The filters installed in the device are not capable of separating gaseoussubstances. Therefore, do not work with or store substances in the device:• which in quantity or concentration are toxic,• if a reaction with other substances may result in hazardous toxic concentrations

or formation of toxic gases,• that may form combustible or explosive mixtures in combination with air.

1.5 Standards and safety regulations

The device complies with the safety requirements of the following standards andguidelines:

• IEC 61010-1 / EN 61010-1• EN 12469 / 2000• DIN 12980 / 2005-06 (model Maxisafe 2020 only)• Low Voltage Directive• EMC Directive


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2. Delivery

2.1 Standard components

Delivery for the safety cabinet includes the following:

Model Safe 2020:• safety cabinet,• drain valve.

Model Maxisafe 2020:• safety cabinet• armrests• stand

All models:• Device documentation:

— operating instructions,— factory test report.

Optional components and accessories are listed as separate items in thedelivery document.

2.2 Acceptance inspection

After the device has been delivered, immediately check the device:• for completeness,• for possible damage.

If the delivery is incomplete or if you detect any transport damage to thedevice, contact the forwarding agency and Thermo Electron LED GmbHimmediately.

2.3 Transport lock and device packaging

Do not transport the device over large distances without transport lock and originaldevice packaging.


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3. Installation

3.1 Ambient conditions

The operational safety and correct function of the unit depend on the locationwhere it is to be operated. The safety cabinet must be operated only atlocations that meet the ambient conditions listed below.

Location requirements:• The electrical system of the device has been designed for an operating height

of up to 2000 m above sea level.• The mains power supply outlets should be out of normal reach to prevent

accidental shut-off. Ideally, the outlets should be installed above the safetycabinet.

• The flooring of the location must be adequately strong and not flammable.• The room in which the device is installed must be of adequately height. For

units not connected to an exhaust system, the distance between the exhaustair opening and the room ceiling must be at least 200 mm (8 in).

• The location must be equipped with an appropriate ventilation system(see Section 3.2.).

• The temperature within the room must be between 15 °C and 40 °C(49 °F and 104 °F).

• The relative humidity in the vicinity of the device must not exceed 90 %.

NOTE - Ambient conditions

If ambient conditions vary from those descri-bed above, please contact Thermo ElectronLED GmbH for assistance in installing the de-vice.

NOTE - Temporary storage

If the device is stored only temporarily (up tofour weeks), the ambient temperature may bebetween -20 °C and +60 °C (-4 °F and +140 °F)at a relative air humidity of up to 90 %. For lon-ger storage periods, the location requirementsapply.

3.2 Room ventilation

The room ventilation should preferably be a ventilation system that complies withthe national requirements for the application.• The inlet air and exhaust air openings of the room ventilation must be located

so that drafts are prevented from impairing the function of the safetycabinet air system.


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Fig. 1Locations in a room

3. Installation

3.3 Correct location

Choose a draft-free location where the safety cabinet does notinterfere with the room traffic.Fig. 1: This figure shows preferred locations for safetycabinets and unsuiable locations, not in accordance with thesafety requirements.Unsuitable locations: The locations [1], [2], and [3] are notsuitable because they are exposed to drafts from windows anddoors.Location [5] is undesirable because it is in range of plant trafficand within the exhaust air range of a ventilation system [4].Preferred locations [6], [7], and [8] are correct because theyare in a draft-free section of the room and not exposed to planttraffic.

3.4 Installation in line

When several devices are to be installed in line, please observethe following:• Make sure that vibrations cannot be transferred between

adjacent units.• Exterior surfaces of the cabinets must always be accessible

for cleaning and disinfection.


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3. Installation

3.5 Transport

Model Safe 2020:Fig. 2: To prevent tilting, always transport the device usinga suitable carrier, even for a transport within a building, andseparate it from the stand.

CAUTION – Danger of tipping over!

For transport, lift the device only using the liftpoints shown in the illustration. Do not load thefloorpan with the weight of the device frame!

CAUTION – Contusion hazard!

When lifting the safety cabinet, never placefingers or hands under the base of the device!

Model Maxisafe 2020:If required, the device stand must be removed from the standto be reinstalled onto the stand for the initial start-up after thetransport (see Section 5.2).

Fig. 2Lift points


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4. Unit description

4.1 Overall view

Model Safe 2020:• Fig. 3a: Plenum assembly [5] with plenum for downflow blower [24] and

plenum for exhaust air blower [2]. The downflow filter and the exhaust air filterare installed directly to the relevant blower. The exhaust air is released intothe environment around the device through the opening [1].

• At the side of the plenum, the optional media supply lines [25] are routed intothe sample chamber [20].

• Electrical box [4] with power supply cable [3]. An RS 232 connection to a PCand two fuse holders are installed at the front of the electrical box.

• Front cover [6] with integral, electrically movable front window [7], operated by:• the pilot switch [26].The gas struts [22] secure the front cover in the open state.

• The light dome [23] with two tubes is part of the front cover assy.• Side panels [9] with two sealed access openings [19]. These can be

equipped with media valves [13].• Internal outlets [10] for power supply of accessories and adapter [11] for mobile

UV device.• Optional UV lamp unit [8] consisting of two UV lamps per side.• Optional stands [12], adjustable in height and with fixed height• Workplate segments [15] with optional arm rests [16]. A one-piece workplate

and special workplates are available options.• Lockable drain valve [17] for installation into floorpan.• Display [21] with alarm system indicators.• Test hoses for the supply unit [18] at the left side of the sample chamber and

for the exhaust unit [14] at the right side of the sample chamber.

NOTE – Test hoses

Do not remove the two test hoses for checkingdownflow and exhaust air.


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4. Unit description

Fig. 3aOverall view / Model Safe 2020


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4. Unit description

Model Maxisafe 2020:• Fig. 3b: Stand [5] with integral prefilter housing [6].• Filter inserts [2] for the prefilter housing. The number of supplied filter

inserts depends on the width of the device. To protect from liquid residues,the filter inserts are oriented slightly sloping from the device backpanel toform a drain duct to the floorpan [3].The filter plates [4] have tongues and grooves and are inserted in anoverlapping pattern from right to left.

• Membrane sleeve or opening for filling with aerosol for testing the filter of thedownflow unit [1] at the left side of the sample chamber and of the exhaustair unit [7] at the right side of the sample chamber.

Fig. 3bOverall view / Model Maxisafe 2020


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4. Unit description

4.2 Safety system

The safety system comprises a combination of protective and alarm systemsthat ensure maximum personal and material protection.

Safety systems:• Vacuum-sealed air system

A vacuum-sealed air system in combination with HEPA filters for downflowand exhaust air forms the basis of the safety system for personal andmaterial protection.

• Personal protectionAir aspired from the exterior along the entire working opening at a constanthigh velocity prevents that: • agents may leak through the working opening of the chamber.As the exterior air pressure around the unit exceeds the pressure of theinternal air system (vacuum sealing), it is ensured that: • agents cannot be released to the exterior in the case of a leak in the

cabinet housing.• Material protection

A steady airflow within the air system ensures that:• a constant downflow allows the HEPA filters to remove contaminants

so that the samples are always surrounded by ultrapure air,• harmful particles are not carried over through the sample chamber

(protection from cross-contamination).• HEPA filters

The downflow (i.e. the air circulating within the device) and the exhaust air(air that is released to the exterior) are cleaned by HEPA filters (HEPA = HighEfficiency Particulate Air Filter).For version Maxisafe 2020, a prefiltering system is used to increase the fil-ter efficiency and to protect the exhaust air and downflow systems.

• Safety lockoutTo protect from UV radiation, the optional UV disinfection routine can be runonly if the front opening is closed. During UV disinfection, the front openingsafety lockout is activated and prevents harmful UV radiation from beingemitted from the sample chamber.

Warning system:• Airflow monitoring

Airflow monitoring determines the velocity of the airflow in the sample chamberas well as the inflow velocity of the air aspired from the exterior through theworking opening. As soon as airflow velocities move above or below aspecified safety value, a signal is transmitted to the alarm system.

• Visual and audible alarm systemThe warning system constantly monitors the safety-relevant device functions:• Inflow velocity of the air aspired from the exterior,• downflow velocity,• working position of the front window.If the warning system detects changes to one of these device functions, itissues:• an audible and a visual alarm signal.


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4. Unit description

• Position monitoringThe position sensors monitor the position of the frontcover as well as the movement of the front window; it willindicate when the front window is in the working position.

• Performance FactorThe Performance Factor (PER) is a value that indicates thesafety state of the safety cabinet. This value is calculatedfrom data determined by the safety system and fromvalues captured empirically by service personnel duringsafety checks. This data is entered into a parameter list ofthe control software and interconnected. The result can beindicated by the display.

4.3 Filter system

Model Safe 2020:Fig. 4a: The filter system consists of two HEPA filters [2] and[5] for the circulating air and for the exhaust air and of a coarsefilter for the aspired air.

HEPA filters: Room air [10] is drawn into the samplechamber through the working opening. In the air duct, roomair and the downflow within the chamber [7] are then blendedto make up the blend air [9]. The blend air is then:• filtered proportionally by the downflow filter [5] and

supplied as ultrapure air [6] evenly into the sample chamberof the device,

• filtered by the exhaust air filter [2] and released asultrapure air [1] to the exterior of the device.

Inlet air protection: The air duct between the sample chamberand the device plenum has an inlet air protection [8] below theworking surface to prevent coarse particles from entering theplenum where they may impair blower [3] und [4] and filterfunctions.

Fig. 4aFilter system with downflowfilter and exhaust air filter /

model Safe 2020


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4. Unit description

Model Maxisafe 2020:Fig. 4b: The filter system consists of a set of HEPA primaryfilter [10] (the number of filters depends on the sample chamberwidth) and two HEPA filters [2] and [5] for downflow air andexhaust air.

HEPA primary filter:The compact size of the filter inserts allows filter replacementwith minimal contamination hazard. The use of the primary filterprotects the downflow and exhaust air filters considerably.The filter inserts used are HEPA filters of Class H14(according to DIN EN 1822). Alternatively, activated carbonfilters can be inserted into the housing to protect from smells.Room air [11] is drawn into the sample chamber through theworking opening. In the air duct above the prefilter, room airand the downflow within the chamber [7] are then blended tomake up the blend air [8]. The blend air is then:• aspirated into the prefilter [10], filtered and fed into the air

duct [9].

HEPA filters: From the air duct, the primary filtered air is:• filtered proportionally by the downflow filter [5] and

supplied as ultrapure air [6] evenly into the sample chamberof the device,

• filtered by the exhaust air filter [2] and released as ultrapureair [1] to the exterior of the device.

Fig. 4bFilter system with primary

filter, downflow filter andexhaust air filter / model

Maxisafe 2020


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4. Unit description

4.4 Controls and display

Fig. 5: The safety cabinet is equipped with two separate controlelements that operate independently of each other:• pilot switch [1].• operating panel [2],The status indicators of the display [2] indicate controloperations initiated with the control elements.

Pilot switch: Use to control all basic functions required for theoperation.

Operating panel: The operating panel [2] has a displaypanel for text or number value output as well as 12 LEDs forvisual indication of the current operating state of the device.All device functions can be controlled via the keys on theoperator panel.

Fig. 5Controls and indicators


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4. Unit description

4.5 Sample chamber access

The sample chamber of the device is accessible via twomodes:

Front cover:Fig. 6a: Manual opening of the front cover [2] allows accessto the complete sample chamber width with an opening heightC. It is generally needed for decontamination and introductionof larger accessories.

NOTE – Front cover lockout

The front cover is equipped with a safetylockout and can only be opened when the frontwindow is completely closed.

Front window:Fig. 6b: The electrically operated front window [1] is made ofmulti-layer safety glass and integral to the front cover frame.It can be raised to a maximum opening height B (480 mm). Toaccess the sample chamber during the work process, the frontwindow must stay in the work position with opening height A(200 mm).

CAUTION – Front window movement!

Do not attempt to move the front windowmanually as otherwise the motor drive may bedamaged.

Lowering the front window when the device is deenergized:The safety feature (optional) is backed up by a battery. Shoulda power failure occur, the pilot switch (see Section 6.4) can beused to lower the front window completely.

Fig. 6aAccess through front cover

Fig. 6bAccess through front window


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4. Unit description

4.6 Device interfaces

Fig. 7a: The standard equipment includes the outlets [10] for internal power supplyas well as the openings [8] on both sides for routing of cables and hoses. All othersupply connections are available as options.Power supply connection: The connection to the power supply system isachieved through a cable with grounding plug [2] at the rear of the electrical box.Contact connection: The front of the box has an RS 232 interface [3] for theconnection to a PC as well as two fuse holders for 5A miniature fuses:[4] for (L),[5] for (N).Internal power supply: There are two electrical outlets (5A) [10] and one UVdisinfection adapter (optional) [9] located in the side walls.Media valves: There are two sealed feed throughs [8] on each side. These maybe used for installation of media valves [7].Media supply lines: Additionally, media can be supplied into the sample chamberthrough three pipes. The inlets [1] are located on top of the housing, theoutlets [6] are placed at the sample chamber backpanel.

Fig. 7aSupply interfaces


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4. Unit description

Disinfection adapter (optional), Fig. 7a: The disinfectionadapter [9] is used to connect a mobile UV radiation device.The adapter is connected to the device control, the UVdisinfection routine with a mobile UV device may be controlledwith the remote control.

Media connections (optional): The media supply unitconsists of three pipes that are routed into the sample chamberthrough the top of the unit. The inlet connections [1] andoutlet connections [6] with thread (R 3/8"), e.g. for media valves[7], are preinstalled and equipped with a sealing plug.The media connections are universal-type connections. Twoequipotential bonding connections are installed at the top ofthe unit and at the stand.

Caution – Combustible gas!

If a gas burner is to be operated in the samplechamber, an appropriate shut-off device for thegas supply system (shut-off valve, solenoidvalve) must be installed.Use only laboratory safety burners in thesample chamber.

External systems: A failure detection systems or gas supplysolenoid valves may be connected to the safety cabinet control.The unit may also be connected to an external ventilationsystem.

4.7 Sample chamber illumination

Fig. 7b: The work space illumination [4] is equipped with twofluorescent tubes [3] that are installed behind the light dome[2].

Fig. 7bSample chamber illumination


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4. Unit description

4.8 UV lamp unit

Fig. 8: The UV lamp unit consists of two lamp housings [2]with two UV lamps each [1] that are integral to the side walls.Both lamp housings are protected by a stainless steel cover[3].By cross-radiation of the UV units, all surfaces will bedisinfected as the shadow zone is reduced.The operating time of the UV lamps is preset.

NOTE – Protection from UV radiation

As a protection from harmful UV radiation, theUV lamps can only be activated if the frontwindow is completely closed.

4.9 Working area

The standard equipment comprises the segmented workplate forthe models Safe 2020 and Maxisafe 2020. Special workpla-tes are available as optional accessories.The workplates or workplate segments are placed onto theframe above the sample chamber floorpan using twosubmerging wire straps as handles.

Fig. 9: The working area A for perfect material protectionextends over the entire width B and depth C of the workplate.The two arm rests [3] are positioned at a distanceD (20 cm) to each other centrically on the workplate [1] or onthe workplate segments. The armrests are installed to thesecond perforation line [2] of the workplate.

NOTE – Spilling liquid

(model Maxisafe 2020 only)

If larger quantities of liquid are spilled acciden-tally while working with the model Maxisafe2020, the segmented filters installed below theworking surface must be checked, and, if re-quired, the safe functioning of the device hasto be inspected by qualified service personnel.

Fig. 8 UV lamp unit

Fig. 9Working area on the workplate, armrests


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5. Start-up

5.1 Initial operation

Prior to initial operation, the safety cabinet must be subjectedto an installation test. Correct assembly and installationperformed by the operator are essential for good start-up.

Model Maxisafe 2020:Upon the initial connection to the power supply system, thedevice control of version Maxisafe 2020 starts an automatic ca-libration routine to determine the parameters for the safety sys-tem of the device.

NOTE – Calibration routine

The calibration routine is no substitite for theinstallation check performed by the servicepersonnel.

5.2 Installing unit and accessories

Model Safe 2020:Device without stand:• Place the device without stand onto a sufficiently stable

substructure so that the weight of the device frame doesnot rest upon the floorpan.

Device with stand:To assemble the (optional) stand and to install the device frameto the stand:1. Fig. 10a: Slide the two crossmembers [2] onto the retaining

angles [3] of the sidemembers [1]. Secure the crossmembersto the two sidemembers using the screws [4].

2. To attach the device frame [5] to the stand [7], install fourAllen screws [6] loosely into the corresponding threadedholes at the underside of the device.

3. Place the safety cabinet [1] onto the stand so that theAllen screws [6] pass through the corresponding holes [8]of the retaining tabs [10].

4. Push the device frame [5] in the grooves [9] of the retainingtabs [10] all the way to the stop.

5. Tighten the four Allen screws [6].

Fig. 10aStand installation model

Safe 2020


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5. Start-up

Model Maxisafe 2020:Fig. 10b: The stand [3] and the prefilter housing form anassembly. To facilitate the installation of the device frame [1],the stand is equipped with a lifting mechanism.The four columns [7] of the stand have threaded rods [6] ontowhich the device frame can be placed and lowered.1. Fig. 10b: Unscrew the threaded rods from their hex disks

[5] by approx. 10 cm and make sure their height is identi-cal on all four columns.

2. Place the safety cabinet onto the threaded rods. Check tosee whether each guide pin [4] is seated perfectly in itsreceptacle at the lower device frame.

3. Screw in each threaded rod at its column in short, evenintervals all the way to the stop of the hex disk.

NOTE – Leveling the device

To level the device, do not use the liftingmechanism but only the adjustable stands ofthe device.

4. Align the working area of the safety cabinet:Place a bubble level onto the workplate and rotate the stands[2] of the stand using a 24-mm wrench until the workplate isexactly horizontal in all directions. For the vertical alignmentof the device stands, proceed from left to right and from rearto front.

Fig. 10bStand installation model

Maxisafe 2020


27

5. Start-up

Drain valve (optional)Fig. 10c: The drain valve [2] is installed into the floorpanopening [1] at the floor at the left front area of the samplechamber.

NOTE – Installation

The optional drain valve must be installed onlyby authorized service personnel.

WARNING – Drain valve lock!

To prevent contaminated liquid from beingaccidentally drained from the floorpan, thedrain valve must be secured using aconventional padlock.

5.3 Levelling the cabinet

The cabinet should be levelled only after it has beenpositioned.1. Remove transport protection (foil) from the workplate or from

the workplate segments.2. Lift the workplate or the workplate segments by the wire

hooks and place it/them onto the front and rear rails in thesample chamber with the wide line of holes facing forward.

3. Device without stand: Place a bubble level onto theworkplate and align the substructure until the bubble levelindicates an exactly horizontal position in all directions.

4. Device with stand: Place a bubble level onto theworkplate and use the four levellers of the stand to effecta level state in all planes.When adjusting the device stand height, proceed from rightto left and from rear to front.

Fig. 10cDrain valve installation


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5. Start-up

5.4 Power supply connection

WARNING – High voltage!

Contact with current-carrying components maycause a lethal electric shock.Before connecting the device to the powersupply system, check plug and power supplycable for possible damage.Do not use damaged components to connectthe device to the power supply system!

Establishing the power supply connection:1. Before connecting the device to the power supply system, check to see if the

voltage of the outlet corresponds with the specifications on the nameplateof the device. If the ratings given for voltage (V) and maximum current (A)are not correct, the device must not be connected to the power supplysystem.

2. Connect the grounding plug of the device to a properly grounded and fusedoutlet.• The outlet must be fused separately using a fusible link T 16 A or using

a circuit breaker B 16.3. Make sure that the power supply line is not subjected to tensile or compressive

force.

Installation of the power supply connection:To protect against accidental switch-off, the outlets for the connection to the powersupply must be located outside the normal hand reaching range and must beaccessible only to auhorized personnel. Ideally, the outlets should be installedabove the safety cabinet.

Connecting the equipotential bonding:If the sample chamber is supplied with media (gas, water, etc.), the on-siteequipotential bonding must be connected to one of the premounted threadedbushings either at the top of the housing or at the stand.

Initialization routine:After the unit has been connected to the power supply system, the device controlruns through a start-up initialization routine and switches the functions to the OFFmode. The safety cabinet is now operational and can be operated using theoperating panel or the pilot switch

Presetting the alarm limits upon initial operation, Maxisafe 2020:

NOTE – Presetting the alarm linits

The alarm limits must be set only upon theinitial operation of the device.


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5. Start-up

After the initialization routine has been completed, the calibration routine is run(only Maxisafe 2020):

• The display shows "CAL".• The routine starts automatically when the device control is in the work mode

(sees Section 6.2) and runs for approx 30 minutes. If the airflow is disturbedduring the run time, the routine is cancelled and restarted automatically.

• At the end of the routine, the determined parameters for the alarm limits aresaved.

NOTE – Initial operation

According to applicable national standardsand regulations, the calibration routine is nosubstitute for a start-up performed by anauthorized service technician.

Setting the clock:After the initialization routine has been run, the clock should be set to theappropriate time zone (see Section 6, Operation).

NOTE – Power supply connection

The safety cabinet should remain connectedto the power supply system at all times toensure that settings for the individual unitconfiguration remain active in the memory. Ifthe power supply is interrupted for more than5 minutes, the time must be reset correctly.After the power supply connection has beenreestablished, the system switches to theoperating mode that had been active last.


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5. Start-up

5.5 RS 232 interface connection

The RS 232 interface has been designed for a cable connectionwith 9-pin connectors and a contact assignment of 1:1.

Connection of the device:1. Turn PC off.2. Fig. 11: Connect the connector of the serial interface

cable (not comprised in the scope of delivery) to thesocket [1] at the supply interface at the front of theelectrical box.

3. Connect the serial interface cable to an unassignedslot COM 1/COM 2 etc. at the PC.

4. Turn PC on.

Transfer protocol:The interface must be configured as follows:Baud: 9600Data bits: 8Parity: noneStop bit: 1Protocol: noneFIFO–puffer(extended modulation): enabled

Occupancy of conductors:Type of connector [X] : 9-Pin SUB-DPin 2: TxDPin 3: RxDPin 5: GND

5.6 UV connection

Fig. 12: UV disinfection adapter (optional) for an external UVdisinfection unit.Voltage: 230 VCurrent: max. 1,1 AConnectors: [1], [2], [3] and PE-sign

Fig. 11RS 232 interface connection

Fig. 12UV connection


31

5. Start-up

5.7 Installation test

Do not operate the device before the installation test has been completed.• The installation inspection of the device must be conducted in accordance

with EN 12469 and additionally according to DIN 12980 for Maxisafe 2020.The cabinet may be operated as a Class II microbiological safety cabinet,in accordance with EN 12469 / 2000, if the device functions or function patternslisted below were checked and if the test results are within the safety valuetolerances specified in Annex F:• Electrical safety test• Inflow velocity test• Downflow velocity test• Leakage test of HEPA filters• Airflow control test

• A repeat test must also be performed after repairs to the device or aftermajor changes (more than 5 cm) to the location of the device.

• The operator must prepare a test report or request a written test report fromthe authorized test service.

NOTE – Safety warranty

The operational safety of the device,particularly the personal and materialprotection, are guaranteed only if all safetyfunctions of the device have been tested andapproved.Thermo Electron LED GmbH will not warrantthe operational safety if the device is operatedwithout performance of the requiredinstallation test or if the installation test andrepeat test are not performed by adequatelytrained and authorized personnel!

NOTE – Device hygiene

The initial start-up with subsequent installationtest does not include any decontaminationmeasures. For operation in the work process,the sample chamber of the device and theaccessories required for the work processmust be disinfected and cleaned in accordancewith the hygiene guidelines set forth for theapplication.


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6. Handling and control

6.1 Display

Fig. 13: The display in the light dome is used as indicator and output instrumentfor:• status messages,• parameter input and output.

6.1.1 Functions of the display components[1] Display segment for numbers and text

Value displaysThe LEDs 2-5 illuminate when the corresponding value is called up:[2] Display time (yellow LED), usually the active standard display[3] Display downflow velocity (yellow LED)[4] Operating hours after last filter replacement (yellow LED)[5] Display Performance Factor (yellow LED)

Keys[6] Potential-free contact for solenoid[7] Set time[8] Move window up[9] Switch internal outlet on/off (the LED [17] indicates that voltage is applied)[10] Move window down[11] Switch UV disinfection on/off (the yellow LED indicates that the UVdisinfection routine has been activated; if the optional UV lamp is not installed,the function of this key is disabled).[12] Switch device on/off (switch-off only in stand-by mode, see Chapter 6.3)[13] Toggle display: Time, downflow velocity, operating hours, performance factor[14] Switch work space illumination on/off

Function displaysThe LEDs 15 - 18 illuminate only when the corresponding value is called up:[15] Ventilation reduced (yellow LED)[16] Potential-free contact activated (yellow LED)[17] Internal power supply activated (yellow LED)[18] UV disinfection routine activated (yellow LED)

Status displaysThe LEDs 19/20 and 21/22 show the operating condition of the device aseither/or conditions:[19] Front window is not in working position (red LED)[20] Front window is in working position (green LED)[21] Airflow is not steady (red LED)[22] Airflow is steady (green LED)


33


Fig. 13Indicator and operating panel


6.1.2 Display during the calibration routineThe routine runs for approx 30 minutes. During this time, the display showsalternatingly cal and the descending time value. If the calibration routinecannot be started due to faults, the display shows cal permanently.

6.1.3 Display in OFF modeIn the OFF mode, the display shows the current time.For the initial start-up of the device, the clock must be set to the correct time zoneand to the corresponding time output (CET mode or AM/PM mode)(see Section 6.3.9).

6.1.4 Display in work modeIn the work mode, the display shows the values of the device data that had beenshown last (see Section 6.3.9.ff):• Time (hours and minutes)• Downflow velocity• Operating hours after last filter replacement• Performance Factor

6.1.5 Power interruptionIf the master PCB of the device detects a power failure or a failure of thepower supply, a warning is issued.

Note – Power failure warning

In case of a power failure, the display goes offafter 10 seconds, then an audible alarm signalsounds for about 30 seconds.After this warning, the device is no longeroperative.


34


The battery operation safety feature (optional) allows the complete lowering ofthe front window after a power failure by pressing the pilot switch.

6.1.6 Display and functions after a power failureAfter the power supply has been reestablished after a power failure, the displayshows the values and functions that were last shown prior to the failure.The functions that had been selected last will be continued where they had beeninterrupted.

6.1.7 Failure messagesFailure messages are shown on the display as text/number combinations withthe codes ER 1 to ER 5. If one of these codes appears on the display, contactTechnical Service immediately.

Error code Fault causeER 1 Pressure sensor 1 / supplyER 2 Pressure sensor 2 / exhaustER 4 BUS errorER 5 RAM error


35


6.2 Description of the operating modes

The following operating modes exist for the device:• Calibration mode (after initial start-up,

version Maxisafe 2020)• OFF mode• Work mode• Window open mode• Standby mode• UV mode

OFF mode: The device is at "idle". Utilized for charging the sample chamberor for cleaning and disinfection, the front cover can be opened when the frontwindow is completely closed.• The air system blowers are switched off.• The sample chamber illumination is available.• The internal power supply within the sample chamber is available:

• If the internal power supply is activated, the yellow status indicatorINTERNAL POWER SUPPLY ACTIVATED is illuminated.

• The display shows the time. If the timer has been activated, the displayalternately shows the current time and the preset switching time for thetimer.

• The contact for the external device connection is not available.

Work mode: Ensures personal and material protection. In this operating mode,the work process is run within the sample chamber. The device is in the workmode when the front window has been moved into the working position and theairflow is steady.• The front window is in the working position:

• The green status indicator FRONT WINDOW IS IN WORKING POSITION isilluminated.

• No audible alarm signal.• The air system blowers are switched on to ensure steady airflow:

• The green status indicator AIRFLOW STEADY is illuminated.• The sample chamber illumination is available.• The power supply for the sample chamber outlets is available:

• If the internal power supply is ON, the yellow status indicator INTERNALPOWER SUPPLY ACTIVATED is illuminated.

• The monitor-contact for the external device connection is available:• If the contact is activated, the yellow status indicator POTENTIAL-FREE

CONTACT ACTIVATED is illuminated.• The display can show values for: Operating hours, downflow velocity, and

exhaust airflow velocity.

Window open mode: Designates the condition when the window is open andlocated outside the working position. To install and remove accessories, the frontwindow can be opened or moved upwards.• The airflow system blowers are switched on:

• The red status indicator AIRFLOW NOT STEADY Is illuminated.• The front window is not in the working position:

• The red status indicator FRONT WINDOW NOT IN WORKING POSITION isilluminated.

• The work space illumination is operational.


36


• The internal power supply is operational:• If the internal power supply is switched on, the status indicator

INTERNAL POWER SUPPLY ACTIVATED illuminates.

Standby mode: For an interruption of the work process, the front window canbe lowered and the sample chamber sealed aerosol-tight. The air systemoutput has been reduced to match the lower air requirement.• The front window is closed:

• The air system operates at reduced output.• The red status indicator FRONT WINDOW IS NOT IN WORKING POSITION is

illuminated.• The yellow status indicator AIRFLOW REDUCED is illuminated.

• The sample chamber illumination is available.• The internal power supply in the sample chamber is available:

• If the internal power supply is ON, the yellow status indicator INTERNALPOWER SUPPLY ACTIVATED is illuminated.

UV mode: For running the UV disinfection routine, the front window is completelylowered to protect against UV radiation. The routine cannot be run until the frontwindow is in the "closed" position.• The UV disinfection routine is activated:

• The yellow status indicator UV DISINFECTION ROUTINE ACTIVATED isilluminated until the preset time for the routine has elapsed. Then, theUV lamps are switched off automatically, and the status indicator isswitched off.

• The sample chamber illumination is available.• The internal power supply in the sample chamber is not available.• The integral UV lamp (optional) is available.• The power supply for the UV disinfection adapter (optional) is available.


37


6.3 Operation system

The device control software automatically determines the temporary operatingstate of the safety cabinet and automatically disables those functions of theoperating panel that are in contradiction to the safety requirements of the switchingstate.

6.3.1 Basic functionsFig.14: The easy operator guidance ensures that all basic functions can becontrolled with only a few operating steps and that the sfety cabinet can be shiftedinto the working mode.

Switching the device into work mode:

• Keep the key depressed until the ready signal sounds

Moving the front window up:

• Press the key for approx. 1 second

When the front window reaches the working position, the movementstops automatically. When the movement starts above the workingposition, the front window stops at the maximal opening position.

Stopping the upward movement:

• Press, then release the key

This function can be selected with any key except

the key.

Fig14Indicator and operating panel


38


Lowering the front window:

• Keep the key depressed

Stopping the downward movement:

• Release the key

Switching the device to OFF mode:


Availability of functions in the different operating modes: x = available

Key

OFF mode Work mode Standby mode

UV mode

X X X X

X X

X X

X X X

X X X X

X X X

X X X X

X X

X

Mode


39


6.3.2 Moving the front window to the working position

1. Raising or lowering the front window:

• Press, then release the key for approx. 1 second

• The red status indicator on the display is illuminated

• The audible alarm signal is on.

2. When the front window reaches the working position, the movement isautomatically stopped.

• The green status indicator on the display is illuminated

• The audible alarm signal is off if the airflow is steady.

3. If the movement starts above the working position, the front window must firstbe lowered below the working position and then be raised again. To lowerthe front window:

• Keep the key depressed

4. To stop the downward movement:

• Release the key

6.3.3 Silencing the audible alarm signalWhen the front window is moved out of the working position or when thepressure sensors detect a safety-relevant change of the airflow velocities, thecorresponding visual and audible alarm signals are issued. The alarm remainsactive until the front window has been moved to the correct working position oruntil the airflow velocity corresponds with the preset values.

CAUTION – Unsafe working!

When the alarm signals are activated, safeworking is no longer ensured.

NOTE – Switching the alarm signals off

If the alarm signals fail to be switched offautomatically, move the front window to theuppermost or lowest position and contact theTechnical Service.


40


6.3.4 Switching the illumination on and offIn each operating mode, the sample chamber illumination can be switched onor off.

• To switch the illumination on or off:

Press, then release the key

6.3.5 Activating and deactivating the internal power supplyAll outlets in the sample chamber can be activated (power supply on) ordeactivated (power supply off) simultaneously.

1. To activate the power supply:


The yellow status indicator is illuminated.

2. To deactivate the power supply:


The yellow status indicator goes off.

6.3.6 Displaying the UV disinfection timeThis value refers to the operating hours of the set run time of the UVdisinfection or of the power supply for the UV disinfection adapter (optional).This display function is only available when the front window is not closed.The device must be switched to work mode.

1. To display the value:


The time value is output in segments of 30 minutes.

2. To deactivate the value display:

The value display is deactivated automatically after 2-3 seconds.

6.3.7 Activating and deactivating the potential-free contact (optional)An external solenoid valve or alarm system that is connected to the safetycabinet control system can generally be enabled only if the device is operatedin the secure work mode. Enable or disable an external system:

1. To activate the contact:


The yellow status display is illuminated.


41


2. To deactivate the contact:


The yellow status display goes off.

NOTE – LED for potential-free contact

If this button is pressed on the operating panel,the LED also illuminates if no external alarmsystem is connected to the device.

6.3.8 Switching the cabinet to OFF modeThe unit can be switched to OFF mode from any other operating mode:


6.3.9 Setting the timeThe current time of the time zone in which the unit operates must be set at thestart-up of the safety cabinet. Two different display modes can be selected:• CET mode (24:00 hours)• AM/PM mode (12:00 hours)When the time is to be set, the device must be in the OFF mode.

1. Set the time:

Keep the key depressed until the two-digit hour disply flashes.

The minute display shows either A, P or no value (CET time display).The time zone is set at the same time as the hour value: First, setthe time zone (sequence: CET, A, P), then set the exact hour value.

2. While increasing or decreasing the hour value in increments, set thetime zone:

Press, then release the or the key

3. Scroll through values:

Keep the or the key depressed

If the keys are depressed for approx. 2 or 3 seconds, a higher scrollspeed is selected.

4. Store the hour and time zone setting:

Press the key

The function switches to minute display (flashing).


42


5. Set the minutes:


6. Scroll through values:


If the keys are depressed for approx. 2 or 3 seconds, a higher scrollspeed is selected.

7. Store the minute value:


The display shows the time.

NOTE – Calling up device data

The following data can be called up insuccession:• Time (hours/minutes)• Downflow velocity• Operating hours after last filter

replacement• Performance Faktor

To call up values in succession:

• Press, then release the key for eachvalue

The following three sections contain detailedinformation about displaying values.

6.3.10 Displaying the downflow velocityThe sensor system of the device continuously monitors the downflow velocityof the airstream in the sample chamber. The currently determined value (m/s)can only be called up in the work mode.

• To display the velocity value:

Press the key repeatedly until the yellow E LED illuminates.


43


6.3.11 Displaying the operating hours of the HEPA filtersThe filter total operating hours after the last filter replacement can bedisplayed. Upon each filter replacement, the hours are reset to zero.

1. To display the total operating hours:

Press the key repeatedly, until the yellow status indicator

on the display is illuminated.

2. The value indicates the full hours.

6.3.12 Displaying the Performance FactorThe Performance Factor (PER) is a value that indicates the safety state of thesafety cabinet.This value is calculated from data determined by the cabinet safety system andfrom values captured empirically by service personnel during safety checks. Thisdata is entered into a parameter list of the control software and interconnected.The result can be indicated by the display.

1. To call up the PER:

Press the key repeatedly until the yellow status indicator

on the display is illuminated.

The PER is displayed as an integer.

2. To evaluate the PER:

Number in the 100 to 60 range: The safety cabinet is operationallysafe. Personal and material protection is ensured.

Number in the 59 to 30 range: The safety cabinet is still operationallysafe. Personal and material protection is ensured.The safety systemshould be checked.

Number in the 29 to 0 range: The safety of the device may beimpaired. The failure causes must only be repaired by authorizedservice personnel. Contact Technical Service.

NOTE – Calling up value

The value should only be called up, when theairflows have stabilized itself after a lead timeof approx. 20 min.


44


6.3.13 Setting and activating the timerThe timer function allows you to switch the safety cabinet from the OFF modeto the work mode at a predetermined time. The timer can only be set when thedevice is in the OFF mode.

1. To set the switching time:

Keep the key depressed until the ready signal sounds

The display flashes the two-digit hour display.

2. Increase or decrease the hour value in increments:


3. Scroll through the value display:

Keep the or the depressed

If the keys are depressed for approx. 2 or 3 seconds, the higherscroll speed is selected.

4. Store the hour setting:


The function switches to minute display (flashing).

5. Set the minute value:





7. Store the minute value and activate the timer:


The status display flashes. After a moment, the display showsthe current time again. When the value is stored, the timer isautomatically activated.

If the setting is not stored, the switching time will be reset to the original valueafter approx. 15 seconds.


45


NOTE – Activating the timer

If the timer is to be activated without a priorchange of the time values, the procedureabove is performed and the existing timevalues are confirmed:

Press, then release the key for eachvalue.

After the minute value has been confirmed, thetimer has been activated.

NOTE – Timer function

The timer can not be used as a start routinethat calls up its function automatically.It must be reactivated separately for eachdeferred device start.

6.3.14 Deactivating the timerIf the device was started at a preset time, the timer function is also deactivatedautomatically. The deferred start can therefore be cancelled only while thedevice is still in the OFF mode.

• To deactivate the timer function for starting the device:


The safety cabinet changes to the work mode.

The status indicator illuminates continuously, and the displayshows the current time.


46


6.3.15 Setting the UV disinfection timeDepending on the equipment option of the cabinet, this setting is used to:• determine the disinfection time of the optional UV lamps in the side walls or• set the time for the power supply of a mobile UV device at the UV adapter.Factory setting is one hour. The time can be set within a range between 0 and24 hours in increments of 30 minutes each. The unit must be in the work mode(the front window must not be closed). For each following start of the UVdisinfection, the routine is run with this preset time value.

1. Select the function:


The display flashes the run time that had been selected last.

2. Set or change the disinfection time. To increase the value inincrements:


3. Scroll through the value display in increments of 30 minutes:


4. Store the setting:


If the setting is not stored, the disinfection time will be reset to the originalvalue after approx. 15 seconds.

6.3.16 Starting the UV disinfectionThe UV disinfection can only be started if the front window is completely lowered(standby mode). Depending on the equipment of the unit version, this functionis used to:

• switch on the optional UV lamps in the side walls or

• activate the power supply for the outlets of the mobile UV device.

• Start the routine:


The display alternately shows the Text UV and the remainingdisinfection time in hours and minutes.

The yellow status indicator is illuminated.

After the disinfection time has elapsed, the current time is displayed.


47


6.3.17 Cancelling the UV disinfectionWhile the UV disinfection routine is run, it can be interrupted at any time.

1. Cancelling the routine:


The status indicator goes off.

2. The display shows the current time.

6.3.18 Activating the stop watchThe stop watch function starts a countdown for a preset time (max. 99 min and59 s) and issues an audible signal when the set time has elapsed. The signalcannot sound if some other device function has already caused an alarm. Thefunction can only be avtivated if the device is in the normal working mode.

1. Select the stop watch function:


2. Set the minutes (0 - 99) in increments:





4. Store the minute setting:


The function switches to second display (flashing).

5. Set the second value (0 - 59):





7. Store the setting and start the stop watch:



48


8. Indication of the function:

The predefined time value counts to zero.

6.4 Pilot switch

Fig. 17: The basic functions required for the operation of thedevice can be controlled with the pilot switch:• switch the device on,• Raising and lowering the front window,• switching the device to OFF mode.

6.4.1 Moving the front windowThe movement of the front window is controlled by depressingthe pilot switch control element with the corresponding arrowsymbol.1. To raise the window, press the control element [1]. When

the front window reaches the working position, themovement stops automatically. If the movement starts abovethe working position, the front window stops at themaximum opening position.

2. To stop the upward movement, release the control element.3. To lower the window, keep the control element [2]

depressed.4. To stop the downward movement, release the control

element.

NOTE – Switching functions (on/off)

If the device is started with the pilot switch, thechamber illumination is switched on.If the device is switched off with the pilotswitch, the chamber illumination is alsoswitched off.

5. If the front window is not in the working position,• the red status indicator FRONT WINDOW IS NOT IN

WORKING POSITION is illuminated.• The audible signal is silenced when the front window

is completely closed.

6. When the front window reaches the working position afterbeing moved upwards, the movement stops automatically:• The green status indicator FRONT WINDOW IS IN

WORKING POSITION is illuminated.• The audible alarm signal is switched off.

Fig. 15Basic functions of the

pilot switch


49


6.4.2 Switching the device to OFF mode1. Move the front window to the upper and lower end positions.2. Release the control element.3. Keep the previously actuated control element depressed until the ready

signal sounds.

NOTE – Switch-off function

If the device is switched to the OFF mode withthe pilot switch, the chamber illumination isswitched off. The device-internal powersupply remains in the last functional state thatit had been switched to.


50

7. Operation

7.1 Hygiene preparations for the sample chamber

The sample chamber surfaces and the accessories required for the workprocess must be disinfected and cleaned in accordance with the hygieneguidelines set forth for the application.

7.2 Preparing the sample chamber

Installing the accessories:1. Lower the front window completely.2. Open the front cover or move the front window into the maximum opening

position.3. Position the accessories in the working area of the workplate.4. Close the front cover.5. Move the front window to its working position and wait until the airflow has

stabilized.

CAUTION – Operational safety!

The personal and material protection is ensuredonly if the airflow system of the device isworking properly.If the alarm system issues failure messageswhen the front window is in the workingposition, stop all applications that may releaseharmful aerosols!

NOTE – Application of cystostatic agents

For the application of cystostatic agents, thelowered single-part working plate must beused. Application of cystostatic agents.

6. Then place samples into the chamber.7. To interrupt the work process or for extensive experiment cycles without

manual interference, switch the device to standby mode. When the workingopening is completely closed, the safety cabinet is sealed aerosol-tight.

7.3 Response to failure messages

Failure messages are displayed in form of text/number combinations with a codebetween ER 1 and ER 5 (see chapter 6.1.6). If one of these codes is displayed,contact the Technical Service immediately.To isolate the cause of the failure, the operating personnel must perform onlythe following tests and measures:• Check to see if the exhaust air opening on top of the cabinet is blocked.• Ensure that the on-site exhaust air system is activated.• Close doors and windows in the laboratory to prevent drafts.• Switch off devices in the vicinity of the safety cabinet that cause air

turbulence or emit excessive heat.• Open flames in the sample chamber may impair airflow conditions.


51

7. Operation

7.4 Work rules

The observance of work rules ensures a minimum of operational safety whenhandling the safety cabinet.

Before starting an operation:• Take off jewelry.• Put on required personal protective gear, e.g. hand, face, or body protection.• Clean and disinfect sample chamber surfaces at regular intervals.

During operationan:• Place samples only with in the defined work area of the workplate.• Do not place unnecessery items into the sample chamber.• Use only disinfected and cleaned accessories for the work process.• Do not cause air turbulence, by quick hand, arm or body movement in the

sample chamber or in front of the work opening.• Do not place accessories into the sample chamber that cause air turbulence

or emit excessive heat.• Do not block air circulation at the ventilation slots of the workplate.

Sitting posture during work:Fig. 16: To prevent risks to health, a height-adjustable workingchair with an adjustable seat back should be used duringextended work periods at the safety cabinet.A When the forearm rests on the armrest, is should be in an

almost horizontal position.B When the thigh is in a horizontal position, the angle between

thigh and lower leg should exceed 90°.

To ensure a compensation between floor and sitting height, aFootrest (optional accessory) should be used.

After finishing an operation:• Remove samples from the sample chamber and store them

properly.• Clean and disinfect the sample chamber surfaces,

including the workplate and the floorpan. Clean anddisinfect all accessories.

Fig. 16Sitting posture


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8. Shut-down

8.1 Interrupting an operation

To interrupt a work process, the device is switched to the OFF mode.1. Remove all samples from the safety cabinet and store them properly.2. Remove accessories from the sample chamber and clean and disinfect them.3. Clean and disinfect the sample chamber surfaces, the workplate, and the

floorpan.4. Switch the device to OFF mode by keeping the ON key depressed until the

indicators go off (a dot in the right display segment remains as a voltage signalindicator).

8.2 Shutting the unit down

If the unit is not to be used or stored for an extended period of time, it must becompletely decontaminated.

CAUTION – Decontamination measures!

To shut the device down, the sample chambermust be disinfected completely and theplenum, including the filters, must be sterilizedusing formaldehyde.

1. After the device has been decontaminated, close the front window completely.2. Disconnect the device from the power supply system.


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9. Cleaning and decontamination

9.1 Decontamination procedure

Several procedures can be applied for decontaminating the safety cabinet. Whichprocedure is selected, depends on:• the potential risk imminent in the agents,• the degree of purity required by an experiment or by a work process.

Possible decontamination procedures:Wipe/spray disinfection: is the standard disinfection procedure for cabinetsused for microbiological experiments.

UV disinfection: is particularly suited as an intensifying additional disinfectionafter a wipe/spray disinfection.

Sterilization with steam: can be used for treating the removable stainless steelcomponents. Examples of autoclavable components are the bezels of the UVlamps, the workplate or workplate segments and the armrests.

Disinfection with formaldehyde: can be performed if a sterile scmplechamber is required for the work process. This sterilization procedure ismandatory:• when filters are replaced,• when the device is shut down,• when the device is discarded.

9.2 Wipe/spray disinfection

The wipe/spray disinfection is performed in three stages:• Predisinfection,• cleaning,• final disinfection

Recommended disinfectants:

NOTE – Compatibility

Chloride-containing disinfectants may damagesome surfaces. Therefore, use only disinfectantsthat do not contain chloride!Disinfectants with an alcohol content of morethan 70 % may cause embrittlement of plasticcomponents after extended esposure. Use onlydisinfectants with a low alcohol content. Whenusing a disinfectant with an alcohol contentof more than 70 %, the release limit of 200 gwithin 2 hours must not be exceeded.Also suited are disinfectants based onquaternary ammonium compounds.


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Predisinfection:1. Remove all samples from the sample chamber and store them properly.2. Remove accessories from the safety cabinet and disinfect them using the

disinfection procedure recommended by the manufacturer.3. The workplate and stainless steel components can be removed from the

sample chamber and disinfected separately.4. For predisinfection, spray disinfectant on all sample chamber surfaces or wipe

the surfaces using disinfectant.5. Do not remove the optional UV lamps from the sockets; wipe them

thoroughly using a damp cloth.6. Switch the device to work mode, move front window to the working

position.7. Allow disinfectant to react as recommended by manufacturer, then operate the

safety cabinet for at least 15 to 20 minutes in the work mode so that releasedaerosols can be absorbed by the filters.

Cleaning:1. Wipe the surfaces clean using a clean cloth and plenty of clear water.2. Remove dirt residues and deposits thoroughly.3. Remove the cleaning liquid from the floorpan and wipe all sample chamber

surfaces dry.

Final disinfection:1. Again, spray disinfectant on all sample chamber surfaces or wipe the

surfaces clean with disinfectant.2. Allow disinfectant to react as recommended by manufacturer.

9.3 UV disinfection after a wipe/spray disinfection

A UV disinfection can be performed either by using the optional integral UV lampsor by using a mobile UV device. The run time of the routine can be preset atthe operating panel.

9.3.1 UV disinfection using the integral UV lampsTo start the UV disinfection routine:

1. Remove the stainless steel covers from the lamp mountings in theside walls.

2. Close the front window completely, the air system operates in thereduced mode (yellow LED illuminated).

3. Start UV disinfection using the key at the operating panel:

Keep the key depressed until the display alternatingly shows

UV and the remaining disinfection time.

To interrupt / cancel the UV disinfection routine:

Keep the key depressed until UV is switched off (the display

shows the operating hours) or open the front window.


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9.3.2 UV disinfection using a mobile UV deviceTo control the disinfection routine of a mobile UV device with the safetycabinet software, ensure that the connecting plug of the UV device iscompatible with the UV disinfection adapter of the safety cabinet.

To start the UV disinfection routine:1. Position the mobile UV device centered on the work area and

connect to the UV disinfection adapter.

2. Close the front window completely, the air system operates in thereduced mode (yellow LED illuminated).

3. Start UV disinfection using the key at the operating panel:

Keep the key depressed until the display alternatingly shows

UV and the remaining disinfection time.

To interrupt / cancel the UV disinfection routine:

Keep the key depressed until UV is switched off (the display shows

the operating hours) or open the front window.

9.3.3 Changing the UV disinfection timeThe UV disinfection time has been preset but can be changed as required:

1. Switch the safety cabinet on and move the front window to theworking position.

2. Keep the key depressed until the preset time is displayed.

3. To extend the time: Press the key . Each pressing of the keyextends the time by 30 minutes.

4. To reduce the time: Press the key (INTERNAL WORK SPACE ILLUMI

NATION). Each pressing of the key reduces the time by 30 minutes.

5. To save the value: Press the key . The display indicates theoperating hours again.


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9.4 Sterilization with formaldehyde

Procedure:For sterilization, formaldehyde is evaporated in the tightly sealed sample chamber.The quantity of the formaldehyde used depends on the sample chamber volumeof the cabinet version to be disinfected (see Technical Data).Per cubic meter of sample chamber volume, at least 5 g formaldehyde must beevaporated with 20 ml water (corresponds with 25 ml of a 20 % formaldehydesolution). The formaldehyde evaporates immediately when its boiling point isreached. The required reaction time is at least 6 hours.After the required reaction time, the formaldehyde should be neutralized byevaporating a 25 % ammonium solution (10 ml per cubic meter of samplechamber volume).Ambient conditions and accessories:The temperature at the cabinet location should be approx 21 °C, the relativehumidity should be between 60 and 85 %. To evaporate the solution, a heatingdevice with a container is required.

NOTE – Disinfection procedure

A sterilization with formaldehyde must beperformed in accordance with the specificationsof NSF 49/1992, Annex G.As this procedure has considerable risks, itmust only be performed by specially trainedand authorized service personnel!

9.5 Cleaning the exterior surfaces

Wipe the exterior surfaces of the device clean using a solution of tepid waterand commercial dishwasher solution. Then, wipe exterior surfaces dry using asoft, clean cloth.

9.6 Cleaning the floorpan

Clean the floorpan using a solution of tepid water and commercial dishwasher.

1. Remove the working plates from the sample chamber.2. Remove dirt residues and deposits thoroughly.3. Wipe the floorpan clean using a clean cloth and plenty of clear water.4. Remove the cleaning liquid from the floorpan and wipe the floorpan surfaces

thoroughly clean.

NOTE – Material residues

After cleaning, make sure that all cleaningmaterials have been removed completely fromthe floorpan.

5. Reinstall the working plates.


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10. Maintenance

10.1 Inspection

PER is the value that indicates the safety state of the safety cabinet bymonitoring various device parameters.• If PER shows a value below 60, the device should be inspected.• Independent of the PER value, the safety cabinet must be inspected on an

annual basis.

The annual inspection comprises the following checks:• Electrical safety in accordance with national regulations.• Functional test of the device.• Checking all components for possible damage.• Checking the filter state.

NOTE – Perforated plate

The Perforated plate at the blanket of the samplechamber serves for the protection of thedownflow filter and prevents refluxing.While scanning the filter surface for leak testthe perforated plate must be built-in.

• Checking the airflow conditions.• Repeat test in accordance with NSF 49/1992, Annex F.

10.2 Service

Filter replacement:As the filter replacement is an interference with the safety system of the device,filters must only be replaced by Thermo Electron LED GmbH or by adequatelytrained and authorized service personnel.

NOTE – Repeat test

Before filters are replaced the disinfection withformaldehyde is mandatory.After the filter replacement, a repeat test mustbe performed in accordance with EN 12469 /2000.

10.2.1 UV lampsThe UV lamps should be replaced after 1500 operating hours:1. Remove cover from lamp housing.2. Rotate the lamp in the socket so that the lamp contacts can be removed from

the groove in the socket.3. Insert the new lamp into the socket and rotate until the contacts engage.4. Replace cover.


58

10. Maintenance

10.2.2 Sample chamber illuminationThe tubes are installed in the front cover bezel.1. Remove the bezel retaining screws and the bezel.2. The luminescent tubes are retained by rotatable sockets. Rotate the tube

carefully to the removal position and remove it from the socket.3. Insert the replacement tube and rotate it to the working position.4. Reinstall the bezel and secure it with the screws.

10.2.3 Replacing the front window sealFig. 17: When the front window seal shows visible sign of wear, the seal mustbe replaced.The sealing is slipped onto the housing frame and secured by an additionalretaining strip at the lower edge of the sample chamber opening.

1. Lower the front window completely and open the front cover.2. Remove the retaining screws [3] and remove the retaining strip [2].3. Remove the seal [1] from the housing frame retaining lip.4. Slip the groove of the new seal onto the housing frame retaining lip and push

it on so that the seal fits evenly along the entire sealing surface.5. Secure the retaining strip with the screws.

Fig. 17Front window seal replacement


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10. Maintenance

10.3 Retrofitting and repairs

External communication systems, e.g. failure report systems or components forsupplying media such as gas solenoid valves can be retrofitted and integratedinto the device control.

NOTE – Retrofitting and repairs

All retrofitting and repair work are interferenceswith the safety system of the unit. Particularlymodifications to the filter system and resultingchanges of the airflow may impair personal andmaterial protection. Such work must be carriedout only by authorized service personnel.


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11. Disposal

11.1 Disposal procedure

Discarded cabinets or unit components contain reusable materials. Allcomponents with the exception of the HEPA filters can be disposed of after havingbeen thoroughly cleaned and disinfected. The HEPA filters must be disposedof in accordance with the applicable national and state regulations for specialsolid waste.

CAUTION – Contamination hazard!

As the device can be used for processing andtreating infectious substances, it may becontaminated.Prior to disposal, the complete device withfilters must be decontaminated by performinga formaldehyde sterilization!

Recyclable materials!

Component Material

Printed circuit boardsEnclosed electrical components coated with various plastics, mounted on epoxy resin-bound boards.

Plastic components, general Note material labelingExterior housing Steel, paintedDevice backpanel Stainless steel/steel, paintedFront cover seal EMPPFront window, side windows Multi-layer safety glassOperating panel and indicator foil

Polyethylene, Polyester

Workplates Stainless steelUV bezels Stainless steelArmrests Stainless steelAccu (safety feature: Lower Front Window)

Nickel/metal hydrid


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11. Disposal

WEEE Compliance:

This product is required to comply with the European Union‘s Waste Electrical& Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with thefollowing symbol:

Thermo Fisher Scientific has contracted with one or more recycling/disposalcompanies in each EU Member State, and this product should be disposedof or recycled through them.Further information on Thermo Fisher Scientific‘s compliance with theseDirectives, the recyclers in your country, and information on Thermoproducts wich may assist the detection of substances subject to the RoHSDirective are available at www.thermo.com/WEEERoHS.


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12. Technical data


63

12. Technical data


64

12. Technical data

Safe 2020 / Maxisafe 2020 0.9 1.2 1.5 1.8

Width mm 1000 1300 1600 1900Depth mmHeight without stand mm

Width mm 900 1200 1500 1800Depth mmHeight mm

Opening in working position mmMax. opening mm

Working area heightFixed stand mmHeight-adjustable stand mm

Stand heightFixed stand mmHeight-adjustable stand mm

Unit height with standFixed rack mmHeight-adjustable stand mm

Side wall feed throughsDiameter mmDistance lower edge mm

Bushing 1 mmBushing 2 mm

Diameter inches R 3/8 R 3/8 R 3/8 R 3/8

Connection 1 mmConnection 2 mmConnection 3 mm

Connection 4 mmConnection 5 mmConnection 6 mm

Outer distance right570470370

Outer distance left370470570

Optional media connections in chamber backpanel

630

Dimensions

Exterior dimensions

Interior dimensions

800 / 8701580

780

Front window~200

750

780

750 - 950

680

Distance from backpanel

250

680 - 880

130

160

2260 - 2460

23

2260


65

12. Technical data

0.9 1.2 1.5 1.8

Rated voltage VBlower voltage V

Power consumption A 7.3 7.3 8.7 8.7Leakage current IEC1010,

mA

Motherboard fusing AOutlet fusing AOn-site fusing A

Power input W 1700 1700 2000 2000

Protection classProtection typeOvervoltage category(IEC 1010, EN 61010)Contamination degree (IEC 1010, EN 61010)

Connecting lines

0.9 1.2 1.5 1.8Pressure media valves bar max. 6

II

2

Connecting linesCable (4.9 m)

IIP 20

PressureSafe 2020 / Maxisafe 2020

2 x T 5 ACircuit breaker B16 / Fuse T 16 A

Power

Protection

48 V / DC

Current

< 3.5

2 x T 16 A

Electrical dataSafe 2020 / Maxisafe 2020Voltage

1/N/PE AC, 230 V - 50/60 Hz


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12. Technical data

0.9 1.2 1.5 1.8

Unit volume m 3 1.1 1.4 1.7 2.1Floorpan l 30 40 50 60

Cabinet kg 170 200 230 280Stand AFS 1 kg 26 26 27 27Stand AFS 2 kg 30 30 31 31Electrically operated stand kg 30 30 31 31

kg

kg 50 75 75 75

0.9 1.2 1.5 1.8

Unit volume m 3 1.1 1.4 1.7 2.1Floorpan l 4 5 6 7.5

Cabinet kg 200 240 280 330Stand AFS 2 kg 30 30 31 31

kg

kg 50 75 75 75

Volume, weights, and loads

Maxisafe 2020

Volume

Max. load on overall working area

Weights

Safe 2020

LoadsMax. load per working area module

Volume

Weights

Loads

Max. load on overall working area

Max. load per working area module

25

25


67

12. Technical data

0.9 1.2 1.5 1.8

Inflow m/sDownflow m/s

Overall volume flow m³/h 910 1220 1520 1820Downflow volume flow m³/h 620 830 1040 1240Exhaust air volume flow m³/h 290 390 480 580

0.9 1.2 1.5 1.8TypeMaterialSeparability in MPPS %

Width mm 915 1220 1525 1830Depth mmHeight mm

Width mm 457 915Depth mm 457 457Height mm 117 117

0.9 1.2 1.5 1.8TypeMaterialSeparability in MPPS %

Width mmDepth mmHeight mm

Separability at 0.3 µm particle size

% 99.999

610457

Separability at 0.3 µm particle size

99.999%

99.995

457

117

Downflow

Abluft

93

99.995

Airflow system

Glas fiber fleece

Air volume

HEPA (H 14 according to DIN EN 1822)

Filters

Airflow velocities

Safe 2020 / Maxisafe 2020

0.45


0.36

575

Primary filter

86.5257

Maxisafe 2020HEPA (H 14 according to DIN EN 1822)

Glas fiber fleece


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12. Technical data

0.9 1.2 1.5 1.8

Max. ambient temperature during operation

°C

Min. ambient temperature during operation

°C

Max. humidity / operation % r.H.Max. gumidity / storage % r.H.

Room temperature 25 °C W 160/200 200/240 270/310 340/420

56 56 58 58

< 2Above room temperature with window closed

dB(A)

Ambient conditions

9590

Humidity

40

10


Noise level

Temperature

Heat dissipation to environment

°K < 2 < 2 < 2

Ergonomics

The noise level was determined in accordance with EN ISO3744. The sound pressure level was measured at a distance of 1 m in front of the working opening. The measurement uncertainty is within a range of ± 2 dB.

Room temperature rise


69

13. Device log

Part number:Serial number: Service number:Location: Operator's note:

Date Signature

Device type:

Work carried out Notes


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Internet: http://www.thermo.com

Documents

Safe 2020 and Maxisafe 2020 BSC - Thermo Fisher Scientific.pdfValid: 01.2009 / 50118069 Operating Instructions Safety Cabinets Safe 2020 Maxisafe 2020