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Safety and efficacy of probiotics- A Microbiological Perspective
Shilpi SharmaDepartment of Biochemical Engineering &
BiotechnologyIndian Institute of Technology Delhi
[email protected](img from detoxplanet.webs.com)
World within us: Gulliver’s travel
• 3-5 pound of microorganisms
sitting in your gut right now! • Digestion• Production of vitamins• Ward off pathogens• Mucosal maturation• Stimulate Immune System
http://germzoo.blogspot.in/2013/01/fecal-transplants-to-treat-difficult.html
• Bacteria in our body outnumber
the number of cells by 10:1.
We are only 10% human!We are never alone!
Probiotics• Live microorganisms• ‘superbugs’• Often regulated as dietary supplements
Factors affecting efficacy of probiotics
• Strain• Dosage• Duration of treatment• Mode of administration• Health status of individual
http://foundingbumps.com/2014/09/17/you-are-what-you-eat/
Current safety parameters• Genetic stability of the probiotic over time, • Deleterious metabolic activities • Potential for pathogenicity or toxicogenicity • Immunological effects
Methodologies• In vitro toxicological assays• Animal models• Cancer cell lines
3R (Replacement, Reduction, Refinement)
Indigenous strains!!!
Questions to be addressed• How different is Indian gut microbiome?• How does the introduced bacteria interact
with resident population?• How transferable are its ‘superbug’ traits to
other non-desirable organisms in gut?• What is the correct dosage, with what
supplement, and for which age group?
http://www.popsci.com/science/article/2011-09/fyi-how-much-bacteria-do-people-carry-around
Way forward…• Characterization of Indian gut microbiome• Indigenous probiotic strains• Untransformed non-cancer cell lines• 3D gut models
Shetty et al 2013http://www.clker.com/clipart-intestinal-bacteria.html
Our contribution• Bio-prospecting for indigenous probiotic strains
with multiple health benefits• Chemostat model for establishment of gut
microbiome• Assessment by monitoring effects of
supplements on gut microbial community
Emulsifiers / Probiotics / Flavor enhancers
Thank you
3D functional cell model of gut
(by Milipore, Billerica, USA taken from Trapecar and Cencic 2012)