UTMB at Galveston 17 th Annual Forum on Aging Poster Session November 12, 2013 37 Basic Science Research-Student PROPHYLACTIC TREATMENT WITH MONOPHOSPHORYL LIPID-A (MPLA) - A TOLL-LIKE RECEPTOR-4 (TLR-4) AGONIST – IMPROVES RESISTANCE TO PSEUDOMONAS INFECTION AND MORTALITY IN YOUNG AND AGED MICE FOLLOWING BURN INJURY. Ashwin Varma 1 , C. Romero 2,3 , Y Wang 2 and M Romezi 3 1 Clear Lake High School; 2 Department of Anesthesiology; 3 School of Medicine, UTMB, Galveston, TX. OBJECTIVE: To determine if young and aged mice receiving MPLA-prophylaxis following large- surface area flame burns will increase resistance to the wound infection. METHODS: C57BL/6 wild-type mice [2-month old (young) and 23-month old (aged); female] received ≥40% surface area burn under anesthesia. On days 02 & 04 post-burn, mice received injection of MPLA (1µg/0.2 cc; ip) or saline (0.2 cc). On day 05, all mice were challenged with lethal inoculum of Pseudomonas aeruginosa (1 x 108 cfu/0.2 cc). Five days post-infection, blood, lungs, spleen and skin wound biopsies were harvested to determine the bacterial load in vivo. Additional control and MPLA-primed young and aged mice were observed for mortality during the next twelve days. RESULTS: Five days post-infection, MPLA-treated mice, both young and aged, had significantly fewer bacteria in their blood, lung and burn wound biopsies. Mice receiving MPLA-prophylaxis showed significantly decreased mortality than control mice. CONCLUSIONS: MPLA-prophylaxis after initial large-burn insult significantly improved clearance of Pseudomonas aeruginosa and reduced mortality compared to saline-treated young and aged mice.

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Published Abstract at the 2013 Sealy Conference for Aging Research

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UTMB at Galveston 17th Annual Forum on Aging Poster Session November 12, 2013

37 Basic Science Research-Student

PROPHYLACTIC TREATMENT WITH MONOPHOSPHORYL LIPID-A (MPLA) - A TOLL-LIKE RECEPTOR-4 (TLR-4) AGONIST – IMPROVES RESISTANCE TO PSEUDOMONAS INFECTION AND MORTALITY IN YOUNG AND AGED MICE FOLLOWING BURN INJURY.

Ashwin Varma1, C. Romero2,3, Y Wang2 and M Romezi3

1Clear Lake High School; 2Department of Anesthesiology; 3School of Medicine, UTMB, Galveston, TX.

OBJECTIVE: To determine if young and aged mice receiving MPLA-prophylaxis following large-surface area flame burns will increase resistance to the wound infection.

METHODS: C57BL/6 wild-type mice [2-month old (young) and 23-month old (aged); female] received ≥40% surface area burn under anesthesia. On days 02 & 04 post-burn, mice received injection of MPLA (1µg/0.2 cc; ip) or saline (0.2 cc). On day 05, all mice were challenged with lethal inoculum of Pseudomonas aeruginosa (1 x 108 cfu/0.2 cc). Five days post-infection, blood, lungs, spleen and skin wound biopsies were harvested to determine the bacterial load in vivo. Additional control and MPLA-primed young and aged mice were observed for mortality during the next twelve days.

RESULTS: Five days post-infection, MPLA-treated mice, both young and aged, had significantly fewer bacteria in their blood, lung and burn wound biopsies. Mice receiving MPLA-prophylaxis showed significantly decreased mortality than control mice.

CONCLUSIONS: MPLA-prophylaxis after initial large-burn insult significantly improved clearance of Pseudomonas aeruginosa and reduced mortality compared to saline-treated young and aged mice.