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HALO® and Ascentis® Express
Advanced Materials Technology
Sigma-Aldrich Biotechnology
10 12 14 16 min
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See thePerformance Difference
1 Decreased secondary interactions with a more inert base material
2 Sharper peaks for greater resolution
Ranitidine
3 Increased sensitivity (S/N ratio) with taller peaks
Conditions for all columns:Columns: Kinetex® 2.6 µm C18 100 Å
HALO® 2.7 µm C18 90 ÅAscentis® Express 2.7 µm C18 90 Å
Dimensions: 100 x 4.6 mmMobile Phase: A: 40 mM Potassium phosphate, pH 7.1 / Acetonitrile (98:2)
B: 40 mM Potassium phosphate, pH 7.1 / Acetonitrile (78:22)
Flow Rate: 1.5 mL/minTemperature: 35 °C
Detection: UV @ 230 nmSample: Ranitidine amino alcohol hemifumarate1.
Ranitidine diamine hemifumarate (Related compound A)2. Ranitidine S-oxide (Related compound C)3. Ranitidine N-oxide4. Ranitidine complex nitroacetamide5. Ranitidine HCl6.
Gradient: Time (min) % B0 010 10015 100
Conditions for all columns:Columns: Kinetex® 2.6 µm XB-C18 100 Å
HALO® 2.7 µm C18 90 ÅAscentis® Express 2.7 µm C18 90 Å
Dimensions: 100 x 4.6 mmMobile Phase: A: 0.1 % Trifluoroacetic acid (TFA) in Water
B: 0.1 % Trifluoroacetic acid (TFA) in AcetonitrileGradient: Time (min) % B
0 33 323 65
Flow Rate: 1.5 mL/minTemperature: 40 °C
Detection: UV @ 214 nmSample: 1. Insulin
2. Sample Impurity3. Sample Impurity4. Sample Impurity
Insulin Impurity Profiling
HALO is a registered trademark of Advanced Materials Technology, Inc. Ascentis is a registered trademark of Sigma-Aldrich Biotechnology LP. All columns used for comparison were new and manufactured by Advanced Materials Technology, Inc. and Sigma-Aldrich Biotechnology LP, respectively. Phenomenex is in no way affiliated with the above companies. Dimensions and chromatographic conditions are the same for all columns compared unless otherwise noted. Comparative separations may not be representative of all applications.© 2011 Phenomenex, Inc. All rights reserved.
HALO®
Advanced Materials Technology
HALO®
Advanced Materials Technology
Ascentis® Express
Sigma-Aldrich Biotechnology
Ascentis® Express
Sigma-Aldrich Biotechnology
Kinetex®
Phenomenex®
Kinetex®
Phenomenex®
1538.3 mAU
3
657 mAU
3
553.7 mAU
3
2 Rs (peaks 3 & 4) = 4.41
2 Rs (peaks 3 & 4) = 1.83
2 Rs (peaks 3 & 4) = 1.76
0 2 4 6 min
0
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mAU
1 2 3 4 5
6
AP
P ID
196
72
AP
P ID
196
77
1 As = 1.05
0 2 4 6 min0
100
200
300
mAU
1 2 3 4 5
6
AP
P ID
196
74
AP
P ID
196
78
1 As = 1.29
0 2 4 6 min0
100
200
300
mAU
1 2 3 4 5
6
1 As = 1.16
AP
P ID
196
73
AP
P ID
196
79
1210
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14 16 min
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10 12 14 16 min
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1
USA Tel: 310-212-0555 Phenomenex | Canada | Tel: 800-543-3681 | Fax: 310-328-7768 | Email: [email protected] | Web: www.phenomenex.com Puerto Rico Tel: 800-541-4752
3
See the Particle Difference
Advanced particle morphology and uniformity
Kinetex® Sub-3 µm Particle HALO® Ascentis® Express2.6 µm Total Particle Size 2.7 µm 2.7 µm
0.35 µm Porous Shell 0.5 µm 0.5 µm1.9 µm Solid Core 1.7 µm 1.7 µm
1000/600 bar* Pressure Limit 600 bar 600 bar100 Å Pore Size 90 Å 90 Å
1.5 – 10** pH Range 2 – 9 2 – 9200 m2/g Effective Surface Area ~225 m2/g ~225 m2/g
Sub-2 µm Particle1.7 µm Total Particle Size - -0.23 µm Porous Shell - -1.25 µm Solid Core - -1000 bar Pressure Limit - -
100 Å Pore Size - -1.5 – 10** pH Range - -200 m2/g Effective Surface Area - -
AP
P ID
187
55 –
1875
7
Nearly monodispersed particle size distribution
Excellent batch-to-batch reproducibility
10
1
2
3
4
5
18755
2 3 4 5 min
18756 18757
Batch 68
Batch 73
Batch 75
Kinetex® 2.6 µm C18
Kinetex® 2.6 µm C18
Kinetex® 2.6 µm C18
3000
2000
Par
ticle
Cou
nt
1000
01 2 3
Particle Diameter (µm)4 5 6
Batch 68 Rs (2,3) 2.00
Batch 73 Rs (2,3) 2.13
Batch 75 Rs (2,3) 2.06
Kinetex® 2.6 µm C18 Core-Shell Traditional Fully Porous C18 1.7 µm HALO® 2.7 µm C18
vs.
Kinetex® DP 90/10 = 1.12
HALO® DP 90/10 = 1.15
HALO is a registered trademark of Advanced Materials Technology, Inc. Ascentis is a registered trademark of Sigma-Aldrich Biotechnology LP. All columns used for comparison were new and manufactured by Advanced Materials Technology, Inc. and Sigma-Aldrich Biotechnology LP, respectively. Phenomenex is in no way affiliated with the above companies. Dimensions and chromatographic conditions are the same for all columns compared unless otherwise noted. Comparative separations may not be representative of all applications.
Phenomenex®
Advanced Materials Technology
HALO®Advanced Materials Technology
Kinetex®Phenomenex®
Phenomenex®
* 2.1 mm ID Kinetex columns are pressure stable up to 1000 bar.
** Columns are pH stable from 1.5 - 10 under isocratic conditions. Columns are pH stable from 2 - 8 under gradient conditions.
PRODUCT
DOES NOT
EXISTPRODUCT
DOES NOT
EXIST
Advanced Materials Technology Sigma-Aldrich Biotechnology
USA Tel: 310-212-0555 Phenomenex | Canada | Tel: 800-543-3681 | Fax: 310-328-7768 | Email: [email protected] | Web: www.phenomenex.com Puerto Rico Tel: 800-541-4752
4
Hydrophobicity:
Polarity:
H-Bonding:
Aromatic Selectivity:
Silanol Activity:
pH Range: 1.5 – 10*USP Classification: L7
Particle Sizes: 2.6 µm & 1.7 µmEffective Carbon Load: 8 %
Low High
C8
TMSTMS
This new phase brings the benefits of core-shell technology to USP L7 and other C8 column methods.
pH Range: 1.5 – 8USP Classification: L43
Particle Sizes: 2.6 µm & 1.7 µmEffective Carbon Load: 9 %
Hydrophobicity:
Polarity:
H-Bonding:
Aromatic Selectivity:
Silanol Activity:
Low High
PFP
TMS
F
F
F
F
F
TMS
This pentafluorophenyl phase incorpo-rates fluorine atoms on the periphery of the phenyl ring to provide unique aromatic and polar selectivity.
Kinetex PFPHydrophobicity:
Polarity:
H-Bonding:
Aromatic Selectivity:
Silanol Activity:
pH Range: 1.5 – 10*USP Classification: L1
Particle Sizes: 2.6 µm & 1.7 µmEffective Carbon Load: 10 %
Low High
XB-C18
Si TMSTMS
This new phase provides superior peak shape and separation of basic compounds.
This unbonded silica phase is used under hydrophilic interaction chromatography conditions to provide the ideal selectivity for the retention and separation of polar compounds.
Hydrophobicity:
Polarity:
H-Bonding:
Aromatic Selectivity:
Silanol Activity:
pH Range: 2.0 – 7.5USP Classification: L3
Particle Sizes: 2.6 µm & 1.7 µm
Low High
HILIC
OO
OHH20
H20H20 H20
H20
H20
H20
H20 H20
H20
H20H20
ACN ACN ACN ACN
ACN ACN ACN ACN
ACN ACN ACN ACN
ACN ACN ACN ACN
HO
HO
OHSi
SiSi
HO
OH
Kinetex HILIC
Hydrophobicity:
Polarity:
H-Bonding:
Aromatic Selectivity:
Silanol Activity:
pH Range: 1.5 – 10*USP Classification: L1
Particle Sizes: 2.6 µm & 1.7 µmEffective Carbon Load: 12 %
Low High
C18
TMSTMS
This phase offers the hydrophobic retention and methylene selectivity chromatographers expect from a C18 column.
Kinetex C18
See Amazing Resolution with Complementary Selectivities
* pH range under isocratic conditions. pH range is 2 – 8 under gradient conditions.
Even more than efficiency, selectivity is the most important parameter for obtaining increased resolution.
Tivadar Farkas, Ph.D., Senior Managing Scientist
NEW! Kinetex® XB-C18
NEW! Kinetex C8
USA Tel: 310-212-0555 Phenomenex | Canada | Tel: 800-543-3681 | Fax: 310-328-7768 | Email: [email protected] | Web: www.phenomenex.com Puerto Rico Tel: 800-541-4752
2010 R&D Award Recipient
HALO is a registered trademark of Advanced Materials Technology, Inc. Ascentis is a registered trademark of Sigma-Aldrich Biotechnology LP. All columns used for comparison were new and manufactured by Advanced Materials Technology, Inc. and Sigma-Aldrich Biotechnology LP, respectively. Phenomenex is in no way affiliated with the above companies. Dimensions and chromatographic conditions are the same for all columns compared unless otherwise noted. Comparative separations may not be representative of all applications. 5
See What Other Scientists are Saying
1 Increased sensitivity, S/N ratio
2 Narrower peak widths
3 Less tailing
Fig. 7. Comparison between the peak shapes of insulin (A) and lysozyme (B) recorded on the Kinetex and Halo columns.
Reprinted from Journal of Chromatography A, Volume 1217, Issue 10, with permission from Elsevier. “Performance of columns packed with the new shell particles, Kinetex-C18,” page 1598, copyright 2010. By Fabrice Gritti, Irene Leonardis, David Shock, Paul Stevenson, Andrew Shalliker, and Georges Guiochon.
0 min
0
1 2 3 4
20
60
90(B)
Ab
s [m
AU
] 205
nm
From the Journal of Chromatography A
0 min
0
1 2 3 4
20
40
60(A)
Ab
s [m
AU
] 205
nm
CH3CN/H20/TFA, 31/69/0.1, v/v/v 295 K, 1.2 mL/min
CH3CN/H20/TFA, 35/69/0.1, v/v/v
295 K, 0.9 mL/min
1
3
2
Recent publications in the Journal of Chromatography A compare Kinetex® core-shell technology columns to other popular UHPLC technologies.
Articles
1. Performance of columns packed with the new shell particles, Kinetex-C18 Journal of Chromatography A, Volume 1217, Issue 10, 5 March 2010, Pages 1589-1603. By Fabrice Gritti, Irene Leonardis, David Shock, Paul Stevenson, Andrew Shalliker, and Georges Guiochon.
2. Performance of columns packed with the new shell Kinetex-C18 particles in gradient elution chromatography Journal of Chromatography A, Volume 1217, Issue 10, 5 March 2010, Pages 1604-1615. By Fabrice Gritti and Georges Guiochon.
3. Comparative study of new shell type (Kinetex), sub-2 μm fully porous and monolith stationary phases, focusing on mass transfer resistance Journal of Chromatography A, Volume 1217, 4 June 2010, Pages 3642-3653. By Erzsébet Oláh, Szabolcs Fekete, Jenö Fekete, and Katalin Ganzler.
Access these and additional journal articles by visiting www.phenomenex.com/info/journalarticles
Never had such a low reduced HETP value
been achieved in column manufacturing
technology. F. Gritti et al. / J. Chromatogr. A 1217 (2010) 1589-1603
HALO™
Advanced Materials Technology
HALO™
Advanced Materials Technology
Kinetex®Phenomenex®
KinetexPhenomenex®
USA Tel: 310-212-0555 Phenomenex | Canada | Tel: 800-543-3681 | Fax: 310-328-7768 | Email: [email protected] | Web: www.phenomenex.com Puerto Rico Tel: 800-541-4752
6
Ordering Information
AQ0-8503
AQ0-8530
KrudKatcher Ultra • Extends Kinetex and UHPLC column lifetimes
• Easy to use
• Pressure rated to 20,000 psi (1,375 bar)
• Extremely low dead volume minimizes sample peak dispersion
The KrudKatcher Ultra filter body houses an integrated 0.5 μm 316 stainless steel depth filter that efficiently removes microparticulates from the flow stream without contributing to system backpressure or dead volume (<0.2 μL).*KrudKatcher Ultra requires 5/16 in. wrench. Wrench not provided.
USA Tel: 310-212-0555 Phenomenex | Canada | Tel: 800-543-3681 | Fax: 310-328-7768 | Email: [email protected] | Web: www.phenomenex.com Puerto Rico Tel: 800-541-4752
Kinetex 2.6 μm Minibore Columns (mm)KrudKatcher Ultra In-Line Filter*
30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 4.6 mm ID$ 579 $ 639 $ 699 $ 749 $ 189/3pk
XB-C18 00A-4496-AN 00B-4496-AN 00D-4496-AN 00F-4496-AN AF0-8497
C18 00A-4462-AN 00B-4462-AN 00D-4462-AN 00F-4462-AN AF0-8497
C8 00A-4497-AN 00B-4497-AN 00D-4497-AN 00F-4497-AN AF0-8497
PFP 00A-4477-AN 00B-4477-AN 00D-4477-AN 00F-4477-AN AF0-8497
HILIC –– 00B-4461-AN 00D-4461-AN 00F-4461-AN AF0-8497
Kinetex 1.7 μm Minibore Columns (mm)KrudKatcher Ultra In-Line Filter*
50 x 2.1 100 x 2.1 150 x 2.1 4.6 mm ID
$ 799 $ 829 $ 869 $ 189/3pk
XB-C18 00B-4498-AN 00D-4498-AN –– AF0-8497
C18 00B-4475-AN 00D-4475-AN 00F-4475-AN AF0-8497
C8 00B-4499-AN 00D-4499-AN –– AF0-8497
PFP 00B-4476-AN 00D-4476-AN 00F-4476-AN AF0-8497
HILIC 00B-4474-AN –– –– AF0-8497
Kinetex 2.6 μm MidBore™ Columns (mm)KrudKatcher Ultra In-Line Filter*
30 x 3.0 50 x 3.0 75 x 3.0 100 x 3.0 150 x 3.0 4.6 mm ID$ 579 $ 639 $ 679 $ 699 $ 749 $ 189/3pk
XB-C18 –– 00B-4496-Y0 –– 00D-4496-Y0 –– AF0-8497
C18 00A-4462-Y0 00B-4462-Y0 00C-4462-Y0 00D-4462-Y0 00F-4462-Y0 AF0-8497
C8 –– 00B-4497-Y0 –– 00D-4497-Y0 –– AF0-8497
PFP 00A-4477-Y0 00B-4477-Y0 00C-4477-Y0 00D-4477-Y0 00F-4477-Y0 AF0-8497
HILIC –– –– –– –– 00F-4461-Y0 AF0-8497
Kinetex 2.6 μm Analytical Columns (mm)KrudKatcher™ Ultra In-Line Filter*
30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 4.6 mm ID$ 579 $ 639 $ 679 $ 699 $ 749 $ 189/3pk
XB-C18 –– 00B-4496-E0 00C-4496-E0 00D-4496-E0 00F-4496-E0 AF0-8497
C18 00A-4462-E0 00B-4462-E0 00C-4462-E0 00D-4462-E0 00F-4462-E0 AF0-8497
C8 –– 00B-4497-E0 00C-4497-E0 00D-4497-E0 00F-4497-E0 AF0-8497
PFP 00A-4477-E0 00B-4477-E0 00C-4477-E0 00D-4477-E0 00F-4477-E0 AF0-8497
HILIC –– 00B-4461-E0 00C-4461-E0 00D-4461-E0 00F-4461-E0 AF0-8497
UHPLC / HPLC Sure-Lok™ High Pressure PEEK™ Male Nut FittingsPart No. Description Unit PriceAQ0-8503 Sure-Lok High Pressure PEEK 1-Pc Nut 10-32,
for 1/16 in. Tubing, 12,000 psi (827 bar) 10/pk $ 175
AQ0-8530 Sure-Lok Fitting Tightening Tool, Aluminum ea 26
t: f:
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All other countries: Corporate Office USA (310) 212-0555 (310) 328-7768 [email protected]
www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at [email protected]
Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.
Trademarks Kinetex and Phenomenex are registered trademarks of Phenomenex in the United States, European Union, and other jurisdictions. MidBore, KrudKatcher, and Sure-Lok are trademarks of Phenomenex, Inc. HALO is a registered trade-mark of Advanced Materials Technology, Inc. Ascentis is a registered trademark of Sigma-Aldrich Biotechnology LP. PEEK is a trademark of Victrex plc.
Disclaimer Phenomenex, Inc. is in no way affiliated with Advanced Materials Technology, Inc. or Sigma-Aldrich Biotechnology LP. Comparative separations may not be representative of all applications.
© 2011 Phenomenex, Inc. All rights reserved.
If you are not completely satIsfIed wIth KInetex core-shell columns, send In your comparatIve data to a sImIlar product wIthIn 45 days and Keep the column for free.
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