TAZOTM

Seed Treatment for Turf

From: TerraMax, Inc.

a better way to grow

●Root Development● Drought Tolerance● Yield in mass• Top growth• Survivability

Looking for Better…

Better Start with TAZOTM

A naturally occurring soil bacteria,Azospirillulm produces plant responses that stimulate lateral branching and root development. Azospirillum also fixes atmospheric nitrogen and passes nitrogen directly to the plant. Supplementing fertility.

What is Azospirillum?

1. Improved drought tolerance from larger root mass2. Improved nutrient uptake from increased

root hairs3. Increased nutrient levels due to fixing of

atmospheric N―supplements applied N4. Quicker stand establishment

Benefits…

Researchers have known about Azospirillum for several years. However, early products weren’t stable enough to provide significant benefits.Google search will find 86,000+ references

Why Haven’t I Heard About Azospirillum?

Bacteria can be sensitive to environmental conditions like heat and moisture. Uninsulated from these elements, their viability may decline before they can impact the crop. Not so with TAZOTM!

In A Word – Survival!

Why is Stability Important?

TAZOTM uses an advanced stabilization technology that keeps more bacteria viable so consumers receive more benefit. Packaged TAZOTM remains viable for over one year. TAZOTMhas been shown to be viable on seed for one year.

How is TAZOTM Different?

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TAZOTM Shelf Life

TAZO Liquid Shelf LifeTemperature in Fahrenheit

0

1

2

3

4

5

6

7

8

Start 1 Wk 3 Wks 6 Wks 12Wks

26Wks

52Wks

CFU

s/m

l (lo

g ba

se 1

0)

708595

Essentially TAZO is applicable to all areas where grass is grown.Variety testing has been conducted on bluegrass, ryegrass – perennial and annual, fescue and bent.

Where Does TAZOTM Perform Best?

Treating grass seed with TAZOTM :

Dry: 1#/cwt TAZOTM Dry5 # packages

Liquid: 8 oz per cwt TAZOTM Liquid2.5 gallon container

TAZO pays in fewer call backs, more satisfied customers and quicker stand establishment

How Much TAZOTM?

Kentucky Blue GrassTAZOTM Vs Control 46 DAP

TAZOTM - DryBluegrass Root Volume

Product

TerraMax has developed a seed treatment product that can be used to deliver microorganisms. This is applied as a powder directly on the seed.

DescriptionVarious concentrations and product formulations were tried in this experiment. Kentucky Bluegrass seed was treated and planted in a soil mixture. The plants were raised under flourescent lights and watered together as needed. Root volumes were measured six weeks after planting

Treatment

Product: TAZO™ - Dry

Application rate: 1% by weight of seed

Kentucky Bluegrass Root Volume

11 +/- 4

21 +/- 8

46 +/- 2

25 +/- 6 23 +/- 7

0

10

20

30

40

50

60

AVG

Volu

me

in c

c

Control Mate AZ 2 Mate 2 AZ 22

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Athletic Field

The next slides represent work done by The next slides represent work done by Glen Glen RehbeinRehbein Companies at an athletic Companies at an athletic field installation for a school in field installation for a school in Minneapolis. Supervised by Mike KellyMinneapolis. Supervised by Mike Kelly

TAZOTM Treated vs. Non treated

Treated

Treated

Treated

Non-treated

Non-treated

TAZOTM Non Treated Non Treated vsvs TreatedTreated

Non Treated vs Treated

TAZOTM Non Treated Non Treated vsvs TreatedTreated

Non Treated vs Treated

TAZOTM Non-Treated vs.Treated

UntreatedBluegrass Ryegrass

14 DAPTreated

Bluegrass Ryegrass14 DAP

TAZOTM Non treated vs. Treated Root Volume

UntreatedBluegrass Ryegrass

14 DAP

TreatedBluegrass Ryegrass

14 DAP

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University Results - UNL

Root mass increase significantRoot mass increase significantTop growth improvedTop growth improvedBluegrass is the genusBluegrass is the genusOther sources confirm similar results on Other sources confirm similar results on other grassesother grassesRate dependent:Rate dependent:

Increase in rate decrease in root massIncrease in rate decrease in root mass

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Average Mass (g) with % Variance over control

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Benefits…..

Increase in root massIncrease in root massIncrease in top growthIncrease in top growthNitrogen fixationNitrogen fixationBetter stand establishmentBetter stand establishmentMore plant vigorMore plant vigor

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Market Opportunity

Sod productionSod productionOverseedingOverseedingSeed treatment for turfSeed treatment for turfHydro SeederHydro SeederBag seedBag seedBulk seedBulk seedRange and pasture grassesRange and pasture grasses

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TAZOTM LiquidSeed Treatment for Grasses and Turf

Contains two strains of Azospirillum Directions for Use:Azospirillum lipoferum 105 per ml Use at a rate of 8 ounces per cwt of seed. Azospirillum brasilense 105 per ml Spray uniformly on clean seed using a

seed treater.

Manufactured by: TerraMax, Inc.7769 95th Street SouthCottage Grove, MN 55016

CAUTION:KEEP OUT OF REACH OF CHILDREN

The purchaser shall be solely responsible for determining the adequacy of this product for their individual and collective needs and application. TerraMax, Inc. makes no express warranties of any kind with respect to this product.

Net weight 21.25 pounds (9.44 kg)2.5 gallons (9.47 liters)

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TAZOTM DrySeed Treatment for Turf

Contains two strains of Azospirillum Directions for Use:Azospirillum lipoferum 104 per gram Use at a rate of 1 percent by weight of seedAzospirillum brasilense 104 per gram for treating turf. Avoid moisture when mixing.Inert carriers 99.6% by weight Mix well with seed to assure even coverage.

Plant as soon as possible after treatment. Avoid breathing dust.

Manufactured by: TerraMax, Inc.7769 95th Street SouthCottage Grove, MN 55016

CAUTION:KEEP OUT OF REACH OF CHILDREN

The purchaser shall be solely responsible for determining the adequacy of this product for their individual and collective needs and application. TerraMax, Inc. makes no express warranties of any kind with respect to this product.

Net weight 5 pound (2.27 kg.)Net weight 5 pound (2.27 kg.)

Where Can I Find TAZOTM?

Scott Dyer970-282-7232scottdyer@earthlink.net

TerraMax Inc7769 95th Street SouthCottage Grove, MN 55016dakterra2@aol.com651-458-4401

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TerraMax TAZOTM

Greenhouse Root Mass Study

Completed by Completed by RochRoch E E GaussoinGaussoin PhdPhdUniversity of Nebraska LincolnUniversity of Nebraska Lincoln

September 06September 06

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Terra Max Greenhouse StudyConducted by UNL

ObjectiveObjectiveTo Evaluate relative root growth rate and To Evaluate relative root growth rate and

total root mass of seed treated with total root mass of seed treated with TazoTazoConclusion:Conclusion:

Root mass increase statistically significantRoot mass increase statistically significantTop growth improvedTop growth improvedBluegrass in trialBluegrass in trialOther sources confirm similar results with Other sources confirm similar results with other grassesother grasses

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Initial Set UpAll 21 Boxes Close Up

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Why Cheesecloth?Cheesecloth added on top of the soil to keep Cheesecloth added on top of the soil to keep moist and prevent drying out. Itmoist and prevent drying out. It’’s also used to s also used to prevent loss of seed during watering. Cloth is prevent loss of seed during watering. Cloth is removed 1 month after planting.removed 1 month after planting.

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15 Days after planting

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30 Days after planting

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Root Tracing

Different colors used to Different colors used to indicate different tracing indicate different tracing days. This shows how days. This shows how much growth can occur much growth can occur between tracings and between tracings and which roots are new to which roots are new to each tracing date.each tracing date.The deepest root was The deepest root was marked by the date the marked by the date the root was traced.root was traced.

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SamplingTaking Sample with Cup Cutter Individual Sample

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Washing the Sample

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Partially Washed off Sample

After washing sample After washing sample with water, some soil with water, some soil still remains. The top still remains. The top growth remains intact growth remains intact and all roots are and all roots are meshed into each meshed into each other. The sample is other. The sample is taken to the lab to be taken to the lab to be further washed.further washed.

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Finished Cleaned Sample

Sample is washed in Sample is washed in lab using finer lab using finer methods and any methods and any remaining soil can be remaining soil can be picked up and picked up and cleaned off. Sample cleaned off. Sample is now ready to be is now ready to be separated.separated.

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Dry 1X Dry 2X Liquid 1X

Separated and Dried Samples

Liquid 2X Spray 1X Spray 2X Control

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Average Mass (g) of Dried Samples

Roots Rhizomes Top Growth

Dry 1X 0.44 0.02 0.48

Dry 2X 0.30 0.07 0.40

Liquid 1X 0.54 0.06 0.60

Liquid 2X 0.29 0.10 0.44

Spray 1X 0.42 0.08 0.35

Spray 2X 0.31 0.04 0.40

Control 0.26 0.08 0.31

Rhizome growth was not statistically different at LSD (0.10)

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Average Mass per TreatmentAverage Mass for Dried Samples

0.000.100.200.300.400.500.600.70

1 2 3 4 5 6 7

Treatment

Mas

s (g

) RootsRhizomesTop Growth