SelexION™ Technology for TripleTOF® Systems Site Planning Guide

SelexION™ Technology

for TripleTOF® SystemsSite Planning Guide

March 2015RUO-IDV-01-0720-D | D5041245 D

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Site Planning GuideSelexION™ TechnologyRUO-IDV-01-0720-D | D5041245 D2 of 24

Chapter 1 Introduction...............................................................................................................................4Customer Site Planner Responsibilities..............................................................................................................................4FSE Responsibilities............................................................................................................................................................5Technical Support..............................................................................................................................................................5

Chapter 2 Site Planning Checklist..............................................................................................................6Customer Information........................................................................................................................................................6Requirements.....................................................................................................................................................................6

Site Layout Requirements............................................................................................................................................6Electrical Requirements................................................................................................................................................7Gas Supply Requirements............................................................................................................................................8Ventilation and Waste-Collection Requirements..........................................................................................................9Environmental Requirements.......................................................................................................................................9Solutions and Equipment Requirements....................................................................................................................10Product Familiarization..............................................................................................................................................10

Comments........................................................................................................................................................................11Exceptions........................................................................................................................................................................11Signoff.............................................................................................................................................................................11

Appendix A Site Requirements................................................................................................................12Site Layout Requirements................................................................................................................................................12

Weights and Dimensions...........................................................................................................................................12Installation Site Clearances........................................................................................................................................12

Electrical Requirements....................................................................................................................................................12Mains Supply Connections.........................................................................................................................................13Mains Supply Fluctuations.........................................................................................................................................13Protective Earth Conductor........................................................................................................................................13Uninterruptible Power Supply or Power Conditioner..................................................................................................14System Electrical Specifications..................................................................................................................................14

Gas Supply Requirements................................................................................................................................................15Optional Gas Generators...........................................................................................................................................18

Ventilation and Waste Collection Requirements..............................................................................................................18Environmental Requirements...........................................................................................................................................20

BioSafety Requirements.............................................................................................................................................20Sound Pressure Level.................................................................................................................................................20Vibration....................................................................................................................................................................21

Customer Supplied Solutions and Equipment..................................................................................................................21

Appendix B Equipment Safety Categories..............................................................................................23

Revision History........................................................................................................................................24

SelexION™ TechnologySite Planning Guide3 of 24RUO-IDV-01-0720-D | D5041245 D

Contents

This guide is for the site planner, the individual responsible for preparing the facility for the installation of theSelexIONTM differential mobility separation technology device on the TripleTOF® 5600+ or 6600 system .

The information in this guide supplements the information contained in the Site Planning Guide for the massspectrometer. The laboratory must meet the conditions detailed in both the mass spectrometer Site PlanningGuide and the SelexIONTM technology Site Planning Guide before the Field Service Employee (FSE) can installthe SelexION technology.

Customer Site Planner ResponsibilitiesComplete the Site Planning Checklist on page 6, in consultation with facilities services personnel (gas,electrical, ventilation, and information technology [IT]), and return it to the FSE before the completion date. Referto Signoff on page 11.

Note: The FSE will follow up if the checklist is not received prior to the scheduled installation date.

• Verify that adequate space and the required shipping facilities are available. Refer to Site LayoutRequirements on page 12.

• Provide all required electrical receptacles. Refer to Electrical Requirements on page 12.

• Provide all required regulators, shut off valves, and gases. Refer to Gas Supply Requirements on page15.

• Provide and install all required vents and ventilation devices. Refer to Ventilation and Waste CollectionRequirements on page 18.

• Verify that the requirements for the operating environment are met. Refer to Environmental Requirementson page 20.

• Provide all required solutions and laboratory equipment, including all fittings, and sample tubing for the liquidchromatography (LC) equipment, unless purchased from AB SCIEX . Refer to Customer Supplied Solutionsand Equipment on page 21.

• When the shipment arrives, inspect the packaging for damage and then contact AB SCIEX Customer Serviceor the local FSE to schedule the installation.


1Introduction

FSE Responsibilities• Review the checklist and discuss outstanding issues with the site planner.

• Test and qualify the system to the specifications in the Installation Checklist and Data Log.

Technical SupportAB SCIEX and its representatives maintain a staff of fully-trained service and technical specialists located throughoutthe world. They can answer questions about the system or any technical issues that might arise. For moreinformation, visit the Web site at www.absciex.com.


Introduction

http://www.absciex.com

Customer Information

Organization

Address

City

Country

Zip/Postal codeTelephone

FaxSite planner contactname

E-mail address

Requirements

Site Layout RequirementsRefer to Site Layout Requirements on page 12.

CompleteRequirement

The measured building clearances can accommodate the equipment and crate dimensions.

If the requirements cannot be met, then contact the FSE.


2Site Planning Checklist

Electrical RequirementsRefer to Electrical Requirements on page 12.

N/ACompleteRequirement

Installation of electrical supplies and fixtures complies with local regulations andsafety standards.

One branch circuit is provided for the SelexIONTM technology. The SelexIONtechnology can be connected to the same branch circuit as the acquisitioncomputer.

The AC mains supply voltage source does not fluctuate more than ±10% fromthe nominal voltage.

The mains supply includes a correctly installed protective earth conductor.

A qualified electrician has determined the appropriate AC mains supplyconfiguration based on the system electrical specifications found in SystemElectrical Specifications on page 14.

Electrical Requirements (International)

Refer to Electrical Requirements on page 12.


Electrical installations use locally approved standard connections and cables.

Electrical Requirements (North America)

Refer to Electrical Requirements on page 12.


The branch circuit for the SelexIONTM technology is 15 A, 200 VAC to 240 VAC(208 VAC typical), 50 Hz or 60 Hz. Receptacles for these branch circuits areequipped with CSA/NEMA 6-15R straight-blade receptacles.


Site Planning Checklist

Gas Supply RequirementsRefer to Gas Supply Requirements on page 15.


Installation of gas supplies and connections complies with local regulations andsafety standards.

Gas 1/Gas 2 is available:• Zero-grade air or an AB SCIEX-recommended gas generator

• Delivery pressure of 105 psi (7.25 bar) maximum with flows up to 26 L/min

Source exhaust gas is available:• Clean, dry, and oil-free air or UHP nitrogen (99.999%) or an

AB SCIEX-recommended gas generator

• Delivery pressure of 55 psi (3.79 bar) minimum with flows up to 25 L/min

Curtain GasTM supply is available:• UHP nitrogen (99.999%) or an AB SCIEX-recommended gas generator

• Delivery pressure of 55 psi (3.79 bar) to 60 psi (4.14 bar) with flows up to18 L/min

CAD gas is available:• Clean, dry, and oil-free air or UHP nitrogen (99.999%) or an

AB SCIEX-recommended gas generator

• Delivery pressure of 60 psi (4.14 bar) maximum with flows up to 1 L/min

DMS Resolution gas supply is available:• UHP nitrogen (99.999%) or an AB SCIEX-recommended gas generator

• Delivery pressure of 55 psi (3.79 bar) to 60 psi (4.14 bar) with flows up to3 L/min



Ventilation and Waste-Collection RequirementsRefer to Ventilation and Waste Collection Requirements on page 18.

CompleteRequirement

Installation of plumbing and ventilation fixtures complies with local biohazard regulationsand safety standards.

Ventilation of the laboratory environment in which the mass spectrometer will be used complieswith local regulations and the air exchange rate is appropriate for the work performed.A minimum of 10 air exchanges/hour is required.

A negative flow vent is provided with a total flow rate capacity of 283 L/min (10 cfm), asmeasured at the inlet to the laboratory ventilation system.

A smooth fitting is provided for the roughing pump, with an outside diameter (o.d.) of 3.2 cm(1.25 inches). The vent is within 1.5 m (60 inches) of the exhaust port on the roughing pumpand at least 1 m (40 inches) above the floor.

A fitting is provided for the source exhaust drain bottle with an o.d. of 2.5 cm (1.0 inch). Thevent is within 1.5 m (60 inches) of the source exhaust drain bottle.

Environmental RequirementsRefer to Environmental Requirements on page 20.


The size of the laboratory is at least 31.7 cubic meters (1120 cubic feet).

An ambient temperature of 18°C to 25°C (64°F to 77°F) is maintained.Over time, the temperature remains within a range of with the rate of the changein temperature not exceeding 2°C (3.6°F) per hour. Ambient temperaturefluctuations exceeding the limits might result in mass shifts in spectra.

Note: Peripheral devices might have different operating environmentrequirements. Confirm the operating environment requirements with the vendorof each peripheral device that will be used with the system.

Relative humidity is 20% to 80%, non-condensing.

Air conditioning provides a minimum of 16 000 Btu/hr (mass spectrometer androughing pump only).



BioSafety Requirements

Refer to BioSafety Requirements on page 20.

CompleteRequirement

The site is not designated as BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4).

Solutions and Equipment RequirementsRefer to Customer Supplied Solutions and Equipment on page 21.

CompleteRequirement

All required solutions and bottles are available.

Product Familiarization


An account has been obtained on the AB SCIEX Learning Portal, and the onlinelearning modules have been completed.Go to www.training.absciex.com and click the link to request an account. Whenyou receive your account information, complete the online learning modules.

System documentation has been obtained and reviewed. Go towww.absciex.com/userguides.

(Optional) A qualified maintenance person (QMP) who is suitably aware of theelectrical and chemical risks associated with servicing laboratory equipment isavailable for review of the service procedures with the FSE.



http://www.training.absciex.com

www.absciex.com/userguides

Comments

Exceptions

Signoff

Completion date(yyyy-mm-dd)

Site planner contact name

I acknowledge that all of the installation requirements, as specified in this document, havebeen met.

Return date(yyyy-mm-dd)

FSE name

FSE e-mail



Site Layout RequirementsReturn to checklist.

Weights and Dimensions

WeightLengthWidthHeightEquipment

458 kg (1010 lbs)140.8 cm(56.5 inches)

82.5 cm (32.5 inches)135.9 cm(53.57 inches)

Mass spectrometer

13 kg (29 lbs)53 cm (21 inches)66 cm (26 inches)19 cm (8 inches)SelexIONTM technology

Installation Site Clearances

Clearance RequiredSide

1 m (39 inches) for service accessFront, left, and right

0.7 m (28 inches) for service accessBack

Electrical RequirementsReturn to checklist.

WARNING! Electrical Shock Hazard. Use only qualified personnel for the installationof all electrical supplies and fixtures, and make sure that all installations adhere tolocal regulations and safety standards.

WARNING! Electrical Shock Hazard. Do not connect the SelexIONTM technology tothe same branch circuit as either the mass spectrometer or the roughing pump.

The total combined system (mass spectrometer, roughing pump, and SelexIONTM technology) power consumptionis 6960 VA (50 Hz or 60 Hz) at 240 VAC.


ASite Requirements

Mains Supply Connections• Provide a branch circuit for the SelexIONTM technology. The SelexION technology can be connected to the

same branch circuit as the acquisition computer.

• For installations outside North America, use locally approved standard connections and cables.

• For installation in North America, use a 15 A branch circuit equipped with NEMA 6-15R straight blade receptacles(208 VAC or 240 VAC).

An external line transformer is not required.

Mains Supply FluctuationsReturn to checklist.

In areas where the mains supply is subject to voltage fluctuations exceeding ±10% of the nominal value (200 VACto 240 VAC), a power conditioner is required. High or low voltages can adversely affect the electronic componentsof the mass spectrometer. Refer to Uninterruptible Power Supply or Power Conditioner on page14.

Note: Peripheral devices might have different mains supply fluctuation limits. Confirm the mains supply fluctuationlimit with the vendor of each peripheral device that will be used with the mass spectrometer.

Protective Earth ConductorReturn to checklist.

WARNING! Electrical Shock Hazard. Do not intentionally interrupt the protectiveearth conductor. Any interruption of the protective earth conductor is likely to makethe installation dangerous.

The mains supply must include a correctly installed protective earth conductor that must be installed or checkedby a qualified electrician before connecting the mass spectrometer.


Site Requirements

Uninterruptible Power Supply or Power ConditionerReturn to checklist.

If the SelexIONTM technology is powered by a UPS or power conditioner, then it must meet the followingspecifications.

Table A-1 UPS and Power Conditioner Requirements

RequirementSpecification

200 VAC to 240 VAC True on line (double conversion)Output voltage

50 Hz or 60 HzFrequency

Pure sine-waveWaveform

3 × nominal currentMinimum peak current

< 3%Output voltage distortion

Circuit breakerOutput protection

8000 VAMinimum power requirement

System Electrical Specifications

ValueSpecification

200 VAC to 240 VACNominal input voltage

+/– 10% of nominalInput voltage fluctuation

50 Hz or 60 HzFrequency

5 AMaximum input current

1200 VAMaximum input power


Site Requirements

Gas Supply RequirementsReturn to checklist.

WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Use onlyqualified personnel for the installation of all gas supplies and connections,and make sure that all installations adhere to local regulations and safetystandards.

CAUTION: Potential System Damage. Regulate each supply separately at the massspectrometer to prevent over pressure damage to the mass spectrometer.

CAUTION: Potential System Contamination. Use compression fittings for gas line connections.Do not use liquid pipe sealant. If threaded fittings must be used, then Teflon tape may beused to seal the threads. Do not use soldered fittings unless the tubing is thoroughlycleaned afterwards.

The TripleTOF® system equipped with SelexIONTM technology requires specific gas flows. Use high-pressure liquidnitrogen dewars or AB SCIEX-recommended gas generators.


Site Requirements

Figure A-1 Recommended Gas Connections

TubingFlow Rate(max)

PressureDescriptionGasItem

1/4 inch (0.64 cm)26 L/minMaximum 105 psi(7.25 bar)

Zero grade airGas 1/Gas 21

1/4 inch (0.64 cm)25 L/min55 psi (3.79 bar) to105 psi (7.25 bar)

House air,zero-grade air, orN2

Source exhaust2

1/4 inch (0.64 cm)18 L/min60 psi (4.14 bar)N2Curtain GasTM

supply3

1/4 inch (0.64 cm)1 mL/min60 psi (4.14 bar)N2CAD gas4

1/8 inch (0.32 cm)3 L/min55 psi (3.79 bar) to60 psi (4.14 bar)

N2DMS Resolutiongas

5

Note: Gas pressures and flow rates must be measured at the inlet to the mass spectrometer.


Site Requirements

Note: Use UHP nitrogen (99.999%) or an AB SCIEX-recommended gas generator.

Note: Under some conditions, using air instead of nitrogen for source exhaust gas might improve sensitivityand signal-to-noise.

Note: When using the NanoSpray® ion source, do not use UHP N2 for Gas 1/Gas 2, as there is an increasedrisk of corona discharge, which can damage the emitter tip.

Note: For CAD gas and the Curtain Gas supply, the input fitting on the gas and vacuum bulkhead is 1/4-inchSwagelok, shown in Figure A-3. For Gas 1/Gas 2 and source exhaust gas, the input fitting is quick-connect,shown in Figure A-2.

Figure A-2 Quick-connect Fitting on the Gas and Vacuum Bulkhead

Figure A-3 Swagelok Connection and Tubing


Site Requirements

Optional Gas GeneratorsReturn to checklist.

Gas generators are available from AB SCIEX. Refer to the manufacturer’s documentation that comes with thegenerator for system specifications. Refer to the manufacturer's documentation for information on the numberand type of outlets required.

Ventilation and Waste Collection RequirementsReturn to checklist.

WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Make surethat the mass spectrometer is properly vented and that good general laboratoryventilation is provided. Adequate laboratory ventilation is required to controlsolvent and sample emissions and to provide for safe operation of the massspectrometer.

WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Use onlyqualified personnel for the installation of plumbing and ventilation fixtures,and make sure that all installations follow local bylaws and biohazardregulations.

CAUTION: Potential System Damage. Do not connect the source exhaust hose to the vent.The connection must be made by a qualified FSE.

• Provide a ventilation system with a total negative flow rate capacity of 283 L/min (10 cfm) at item 1 in FigureA-4 and a minimum of 10 air exchanges per hour.

• Provide a smooth fitting for the roughing pump, with an outside diameter (o.d.) of 3.2 cm (1.25 inches). Thevent must be within 1.5 m (60 inches) of the exhaust port on the roughing pump, and at least 1 m (40 inches)above the floor. Refer to Figure A-4.

• Provide a fitting for the source exhaust drain bottle with an o.d. of 2.5 cm (1.0 inch). The vent must be within1.5 m (60 inches) of the source exhaust drain bottle. Refer to Figure A-4.


Site Requirements

Figure A-4 Vent Connections

DescriptionItem

Vent1

Source exhaust hose: 2.5 cm (1 inch) inside diameter (i.d.)2

Roughing pump exhaust hose: 3.2 cm (1.25 inches) i.d.3

Source exhaust drain bottle. The bottle is shown outside of the bottle holder to illustrate tubingconnections. Make sure that the bottle is secured at all times to prevent spills.

4

Source exhaust drain tubing: 1.6 cm (0.625 inch) i.d.5

Roughing pump vacuum inlet hose6


Site Requirements

Environmental RequirementsReturn to checklist.

• Minimum room size of 31.7 cubic meters (1120 cubic feet)

• An ambient temperature of 18°C to 25°C (64°F to 77°F)Over time, the temperature must remain within a range of 2°C (3.6°F) from the temperature at the time of lastcalibration, with the rate of the change in temperature not exceeding 2°C (3.6°F) per hour. Ambient temperaturefluctuations exceeding the limits might result in mass shifts in spectra.

• Relative humidity from 20% to 80%, non-condensing

• Cooling and venting for the mass spectrometer and roughing pump: 16 000 Btu/hr

Note: Operation of the mass spectrometer at altitudes higher than 2000 m (6400 feet) above sea level mightimpact mass spectrometer operation.

BioSafety RequirementsReturn to checklist.

The site must not be designated BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4). AB SCIEX does not install,service, or repair AB SCIEX mass spectrometers in areas designated BSL-3 or BSL-4.

Sound Pressure LevelReturn to checklist.

ValueSound Pressure

69 dBAAverage sound pressure level for the mass spectrometer, roughing pump, and SelexIONTM

technology

Note: Sound levels are for the 5600+ or 6600 system without the optional enclosure.

A sound pressure level of 85 dBA above a reference sound pressure of 20 μPa is at present regarded by manyauthorities as the threshold at which a hazard may be caused. Special means, such as the use of protective earpieces, can make a higher sound pressure level non-hazardous to the operator.

AB SCIEX recommends that the sound pressure level be measured or calculated by the user both at the operator'sposition in normal use and at whatever point 1 m from the enclosure of the equipment has the highest soundpressure level.


Site Requirements

VibrationReturn to checklist.

• The packaged mass spectrometer was tested to confirm that the effects of transportation do not affect massspectrometer performance upon arrival at the customer destination.

• The mass spectrometer is designed to operate in any laboratory environment that accommodates precisionanalytical instrumentation.

Customer Supplied Solutions and EquipmentReturn to checklist.

WARNING! Toxic Chemical Hazard. Refer to chemical product Safety Data Sheetsand follow all safety guidelines when handling, storing, and disposing of chemicals.

In addition to the standard solutions required for the mass spectrometer, you must provide the following solutionsfor use with the SelexIONTM technology.

• MS-grade acetonitrile

• MS-grade methanol

• MS-grade acetone (2 L)

• MS-grade isopropanol (2 L)

Table A-2 Modifier Consumption @ 20 psi Curtain GasTM Flow Rate (µL per minute)

ConcentrationModifier

10%3.0%1.5%

962.558267.928131.924Methanol

1250.425348.056171.378Acetonitrile

1821.856507.115249.696Isopropanol

1746.182486.051239.324Acetone


Site Requirements

Table A-3 Modifier Consumption @ 20 psi Curtain Gas Flow Rate (mL per hour)


10%3.0%1.5%

57.75316.0767.915Methanol

75.02520.88310.283Acetonitrile

109.31130.42714.982Isopropanol

104.77129.16314.359Acetone

Table A-4 Modifier Consumption @ 20 psi Curtain Gas Flow Rate (mL per 24 hours)


10%3.0%1.5%

1386.083385.817189.971Methanol

1800.611501.201246.784Acetonitrile

2623.473730.245359.562Isopropanol

2514.502699.913344.627Acetone


Site Requirements

CategoryDescription

Pollution Degree 2Equipment pollution degree

Overvoltage Category IIMains supply transient overvoltage

Note: Environments with a Pollution Degree 2 rating include laboratories and sales and commercial areas.

For more information, refer to the International Electrotechnical Commission standards IEC 61010-1 and IEC 60364.


BEquipment Safety Categories

DateReason for ChangeRevision

July 2014First release of document.A

October 2014Corrected the relative humidity range.B

January 2015Updated for TripleTOF® 6600.C

March 2015Added environmental and ventilation requirements.D


Revision History

Documents

SelexION™ Technology for TripleTOF® Systems Site Planning Guide